pde5a cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	pde5a
Entrez Gene ID	101062977
Full Name	phosphodiesterase 5A
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003966740.2	XP_003966789.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
XM_029840309.1	XP_029696169.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
XM_029840312.1	XP_029696172.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X5
XM_029840310.1	XP_029696170.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
XM_029840311.1	XP_029696171.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X4
XM_011606477.1	XP_011604779.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
XM_003966740.3	XP_003966789.1	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2

Pathways from BioSystems
Gene Ontology

The following pde5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pde5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo05705	XM_003966740.2 Latest version!	Takifugu rubripes phosphodiesterase 5A, cGMP-specific (pde5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OTo20016	XM_011606477.1 Latest version!	Takifugu rubripes phosphodiesterase 5A, cGMP-specific (pde5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OTo71195	XM_029840309.1 Latest version!	Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OTo71196	XM_003966740.3 Latest version!	Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OTo71197	XM_029840310.1 Latest version!	Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OTo71198	XM_029840311.1 Latest version!	Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OTo71199	XM_029840312.1 Latest version!	Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pde5a ( XM_003966740.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003966789.1 Takifugu rubripes phosphodiesterase 5A, cGMP-specific (pde5a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo05705
Clone ID Related Accession (Same CDS sequence)	XM_003966740.2
Accession Version	XM_003966740.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071000.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 16, 2015 this sequence version replaced XM_003966740.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACT GTTGCTACCC GGAAAGCGAG AGGATGGAAG CGTGGCTGGA CGACCACTTG 
GAGTTCACGC GCTCCTATTT TATCAGGAAG GCGTCAAGGG AGATGGTCAA CGCCTGGTTC 
GCTGAGCGAG TCCACTCCAT CCATCCTTCG GCTCCTAAAG AGAATCCTCC TCAGCATCCC 
AGGACCCAGC AGTCCTCTGC AGACTCTGCT GTCTCCTCAG CCTCCGCCGT CCCCCCGCCC 
ACCATCACCC TGCCGGACTA CTGCTGCCCC TCTCCAGTCA CAGCCCCCAG CCCCGTCCCC 
GGCACCCCGA CTCGTAAAAT CTCAGCTTCG GAATTTGACC GCCCGCTCAG ACCCATTGTG 
GTCAAAGACG CCGAGGGTTC TTTGACGTTC CTAAGCGACG CCGACCGCGA GACTGTCCCC 
GTGCGGGCTG CAGCGATGGG CGAGGGCGGA GGAGTCCACG TCGGGGGTTG CGACGAGGCA 
GGTAGTTGGT GCGGGCGCGA TCGCTGCGCG AGGCTGCTGG AGCTGGTGAA GGAAGTGTCG 
AGTCATCTGG ATGTGACAGC ACTCTGCCAC AAGATCTTCC TCCACATCAA CGAGCTGATC 
GCTGCAGACC GCTACTCGCT CTTCCTGGTC GGCGAGGACA GCTCCAATCG AAAGTTTTTG 
GTCAGCCGCC TGTTTGATGT GGCCGAGGGA TCCACGGTGG AGGAGTCCTC CAGTAGCTGC 
ATCCGGCTGG AGTGGAACAA AGGCATTGTG GGACATGTAG CTGCCACAGG ACAGCCGCTC 
AACATCAAGA ACGCTTACGA GGACTCCCGC TTTAACGCCG AGGTGGACCT GATCACGGGT 
TACAAAACCC ACAGCATCCT GTGTCTGCCA ATCAAGAACC ACCGAGACGA GGTGGTCGGA 
GTAGCTCAGG CCATCAATAA AAAAAGTGGA GATGACGCTG CCTTCACTGA CCAGGACGAA 
AAGGATTTCT CTGCCTATTT GGCCTTTTCC GGCATCGTCC TCCACAACGC TCAGCTCTAC 
GAGACGTCCC AGCTGGAAAA CAGACGTAAC CAGGTGCTGT TGGACCTCGC CAGCCTCATC 
TTCGAGGAGC AGCAGTGCCT GAAGGTTTTA CTCCGGAAGT TTGCGGGAAC CATCTTGTCC 
TTCATGCAGG CCCAGGCGTG CACGGTCTTC ATCGCCGACG AGGACTCTAT GAACTCCTTC 
TCCAGTGTCT TTCACATGGA ATACGAGGAG TTGGGGGAAG TCCTGGATGT CCCAAAAAGG 
GACTGTGATG TCAGCCAGAT CAACTACATG TATGCTCAGT ATGTCAAGAA CACCATGCAG 
ACGCTCAACA TTGCAGATGT GACCAAGGAC CAGCGCTTCC CCTGGACAGG GGAGAATCCA 
GATCACACCA ATGACCAGAT CAAGAGTTTG CTCTGCACTC CCATCAGGAA CGGAAAGAAG 
GACAAAGTGA TCGGTGTGTG TCAGCTGGTG AACAAGATGG ACGAAGCCTC GGACAGTGTG 
AAGGCTTTCA ACAGGAACGA TGAGCAATTC CTGGAGGCTT TTGCCATCTT CTGCGGCCTC 
GGCATCCAGA ACACACAGAT GTACGAGACG GTGGAGAGAG CCATGGCCAA ACAGGAAGTC 
ACCCTGGAGG TCTTGTCATA TCACGCCACT GCCTCCGAGG AAGAATCGCG GGAACTCCAG 
GTAACCGTGG TCGCCACGGT GCCGTCCGCT CAGTCACTGC GGCTTCTGGA CTTCAGCTTT 
AGCGACTTCG AGTTGTCCGA CACGGAAACC ACGCTGGCCA CCATCCGCAT GTTCGTCGAC 
CTCAACCTGG TCCAGAACTT CCAGATGAAG TACATGAATT TATGTCGATG GGTTCTGAGT 
GTGAAGAAGA ACTACAGGAA GAGCGTGGCG TACCACAACT GGAGACACGC CTTCAACACG 
GCTCAGAGCA TGTTTGCTCT CCTCAAGTCT GGACGCTTGC AGAACAACCT GAACGACCTG 
GAGGTCTTAG CTCTGATGAT TGCCACTCTG AGCCACGACC TGGACCACAG AGGAGTCAAC 
AACTCCTACA TACAGAGGAG TGACCACCCA TTAGCCCAGC TCTACTGCCA CTCCACCATG 
GAGCATCACC ACTTCGACCA CTGCCTCATG ATCCTCAACA GCCCTGGAAA CCAGATCCTG 
AGCAGCCTGT CCCTGGACGA GTACAAATCC ACTCTGAAAA TGATCGAGAG GGCTATTCTG 
GCCACGGACC TGGCCCTGTA CATGAAGCGG CGTGGGGAGT TCTTTGAGCT CACCAAGAAC 
AGTCAGTTTG TCTGGGAAGA TGAGCAGCAC AGAGACTTAC TGAGGTCAAT GCTGATGACT 
GCATGTGACA TCTCAGCCAT CACCAAACCC TGGCCCGTGC AACAAAGGAT TGCAGAACTG 
GTGGCGACCG AGTTCTTCGA GCAGGGAGAT AAGGAAAGAC GCGAGCTGAA CATTGAACCC 
AGCGACCTGA TGAATCGAGA GAAAAAAGAC AAGATCCCCA GCATGCAGGT GTCCTTCATT 
GACGCCATCT GTACCCAGTT ATACGAGACA TTGGCCGGCA TGTCGGAGTA CTGCTCCCCA 
CTTTTGGAGG GCTGTCAGAA AAATCGACAG CAGTGGAAGC ATTTGGCCGA AGAGTGCGAG 
AAAGGATTGG TCAACGGCTT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003966789.1
CDS	97..2763
Translation	MEDCCYPESERMEAWLDDHLEFTRSYFIRKASREMVNAWFAERVHSIHPSAPKENPPQHPRTQQSSADSAVSSASAVPPPTITLPDYCCPSPVTAPSPVPGTPTRKISASEFDRPLRPIVVKDAEGSLTFLSDADRETVPVRAAAMGEGGGVHVGGCDEAGSWCGRDRCARLLELVKEVSSHLDVTALCHKIFLHINELIAADRYSLFLVGEDSSNRKFLVSRLFDVAEGSTVEESSSSCIRLEWNKGIVGHVAATGQPLNIKNAYEDSRFNAEVDLITGYKTHSILCLPIKNHRDEVVGVAQAINKKSGDDAAFTDQDEKDFSAYLAFSGIVLHNAQLYETSQLENRRNQVLLDLASLIFEEQQCLKVLLRKFAGTILSFMQAQACTVFIADEDSMNSFSSVFHMEYEELGEVLDVPKRDCDVSQINYMYAQYVKNTMQTLNIADVTKDQRFPWTGENPDHTNDQIKSLLCTPIRNGKKDKVIGVCQLVNKMDEASDSVKAFNRNDEQFLEAFAIFCGLGIQNTQMYETVERAMAKQEVTLEVLSYHATASEEESRELQVTVVATVPSAQSLRLLDFSFSDFELSDTETTLATIRMFVDLNLVQNFQMKYMNLCRWVLSVKKNYRKSVAYHNWRHAFNTAQSMFALLKSGRLQNNLNDLEVLALMIATLSHDLDHRGVNNSYIQRSDHPLAQLYCHSTMEHHHFDHCLMILNSPGNQILSSLSLDEYKSTLKMIERAILATDLALYMKRRGEFFELTKNSQFVWEDEQHRDLLRSMLMTACDISAITKPWPVQQRIAELVATEFFEQGDKERRELNIEPSDLMNREKKDKIPSMQVSFIDAICTQLYETLAGMSEYCSPLLEGCQKNRQQWKHLAEECEKGLVNGLV

Target ORF information:

RefSeq Version	XM_003966740.2
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphodiesterase 5A, cGMP-specific (pde5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003966740.2

ORF Insert Sequence:

ATGGAGGACT GTTGCTACCC GGAAAGCGAG AGGATGGAAG CGTGGCTGGA CGACCACTTG 
GAGTTCACGC GCTCCTATTT TATCAGGAAG GCGTCAAGGG AGATGGTCAA CGCCTGGTTC 
GCTGAGCGAG TCCACTCCAT CCATCCTTCG GCTCCTAAAG AGAATCCTCC TCAGCATCCC 
AGGACCCAGC AGTCCTCTGC AGACTCTGCT GTCTCCTCAG CCTCCGCCGT CCCCCCGCCC 
ACCATCACCC TGCCGGACTA CTGCTGCCCC TCTCCAGTCA CAGCCCCCAG CCCCGTCCCC 
GGCACCCCGA CTCGTAAAAT CTCAGCTTCG GAATTTGACC GCCCGCTCAG ACCCATTGTG 
GTCAAAGACG CCGAGGGTTC TTTGACGTTC CTAAGCGACG CCGACCGCGA GACTGTCCCC 
GTGCGGGCTG CAGCGATGGG CGAGGGCGGA GGAGTCCACG TCGGGGGTTG CGACGAGGCA 
GGTAGTTGGT GCGGGCGCGA TCGCTGCGCG AGGCTGCTGG AGCTGGTGAA GGAAGTGTCG 
AGTCATCTGG ATGTGACAGC ACTCTGCCAC AAGATCTTCC TCCACATCAA CGAGCTGATC 
GCTGCAGACC GCTACTCGCT CTTCCTGGTC GGCGAGGACA GCTCCAATCG AAAGTTTTTG 
GTCAGCCGCC TGTTTGATGT GGCCGAGGGA TCCACGGTGG AGGAGTCCTC CAGTAGCTGC 
ATCCGGCTGG AGTGGAACAA AGGCATTGTG GGACATGTAG CTGCCACAGG ACAGCCGCTC 
AACATCAAGA ACGCTTACGA GGACTCCCGC TTTAACGCCG AGGTGGACCT GATCACGGGT 
TACAAAACCC ACAGCATCCT GTGTCTGCCA ATCAAGAACC ACCGAGACGA GGTGGTCGGA 
GTAGCTCAGG CCATCAATAA AAAAAGTGGA GATGACGCTG CCTTCACTGA CCAGGACGAA 
AAGGATTTCT CTGCCTATTT GGCCTTTTCC GGCATCGTCC TCCACAACGC TCAGCTCTAC 
GAGACGTCCC AGCTGGAAAA CAGACGTAAC CAGGTGCTGT TGGACCTCGC CAGCCTCATC 
TTCGAGGAGC AGCAGTGCCT GAAGGTTTTA CTCCGGAAGT TTGCGGGAAC CATCTTGTCC 
TTCATGCAGG CCCAGGCGTG CACGGTCTTC ATCGCCGACG AGGACTCTAT GAACTCCTTC 
TCCAGTGTCT TTCACATGGA ATACGAGGAG TTGGGGGAAG TCCTGGATGT CCCAAAAAGG 
GACTGTGATG TCAGCCAGAT CAACTACATG TATGCTCAGT ATGTCAAGAA CACCATGCAG 
ACGCTCAACA TTGCAGATGT GACCAAGGAC CAGCGCTTCC CCTGGACAGG GGAGAATCCA 
GATCACACCA ATGACCAGAT CAAGAGTTTG CTCTGCACTC CCATCAGGAA CGGAAAGAAG 
GACAAAGTGA TCGGTGTGTG TCAGCTGGTG AACAAGATGG ACGAAGCCTC GGACAGTGTG 
AAGGCTTTCA ACAGGAACGA TGAGCAATTC CTGGAGGCTT TTGCCATCTT CTGCGGCCTC 
GGCATCCAGA ACACACAGAT GTACGAGACG GTGGAGAGAG CCATGGCCAA ACAGGAAGTC 
ACCCTGGAGG TCTTGTCATA TCACGCCACT GCCTCCGAGG AAGAATCGCG GGAACTCCAG 
GTAACCGTGG TCGCCACGGT GCCGTCCGCT CAGTCACTGC GGCTTCTGGA CTTCAGCTTT 
AGCGACTTCG AGTTGTCCGA CACGGAAACC ACGCTGGCCA CCATCCGCAT GTTCGTCGAC 
CTCAACCTGG TCCAGAACTT CCAGATGAAG TACATGAATT TATGTCGATG GGTTCTGAGT 
GTGAAGAAGA ACTACAGGAA GAGCGTGGCG TACCACAACT GGAGACACGC CTTCAACACG 
GCTCAGAGCA TGTTTGCTCT CCTCAAGTCT GGACGCTTGC AGAACAACCT GAACGACCTG 
GAGGTCTTAG CTCTGATGAT TGCCACTCTG AGCCACGACC TGGACCACAG AGGAGTCAAC 
AACTCCTACA TACAGAGGAG TGACCACCCA TTAGCCCAGC TCTACTGCCA CTCCACCATG 
GAGCATCACC ACTTCGACCA CTGCCTCATG ATCCTCAACA GCCCTGGAAA CCAGATCCTG 
AGCAGCCTGT CCCTGGACGA GTACAAATCC ACTCTGAAAA TGATCGAGAG GGCTATTCTG 
GCCACGGACC TGGCCCTGTA CATGAAGCGG CGTGGGGAGT TCTTTGAGCT CACCAAGAAC 
AGTCAGTTTG TCTGGGAAGA TGAGCAGCAC AGAGACTTAC TGAGGTCAAT GCTGATGACT 
GCATGTGACA TCTCAGCCAT CACCAAACCC TGGCCCGTGC AACAAAGGAT TGCAGAACTG 
GTGGCGACCG AGTTCTTCGA GCAGGGAGAT AAGGAAAGAC GCGAGCTGAA CATTGAACCC 
AGCGACCTGA TGAATCGAGA GAAAAAAGAC AAGATCCCCA GCATGCAGGT GTCCTTCATT 
GACGCCATCT GTACCCAGTT ATACGAGACA TTGGCCGGCA TGTCGGAGTA CTGCTCCCCA 
CTTTTGGAGG GCTGTCAGAA AAATCGACAG CAGTGGAAGC ATTTGGCCGA AGAGTGCGAG 
AAAGGATTGG TCAACGGCTT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde5a ( XM_011606477.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011604779.1 Takifugu rubripes phosphodiesterase 5A, cGMP-specific (pde5a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo20016
Clone ID Related Accession (Same CDS sequence)	XM_011606477.1
Accession Version	XM_011606477.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071000.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACT GTTGCTACCC GGAAAGCGAG AGGATGGAAG CGTGGCTGGA CGACCACTTG 
GAGTTCACGC GCTCCTATTT TATCAGGAAG GCGTCAAGGG AGATGGTCAA CGCCTGGTTC 
GCTGAGCGAG TCCACTCCAT CCATCCTTCG GCTCCTAAAG AGAATCCTCC TCAGCATCCC 
AGGACCCAGC AGTCCTCTGC AGACTCTGCT GTCTCCTCAG CCTCCGCCGT CCCCCCGCCC 
ACCATCACCC TGCCGGACTA CTGCTGCCCC TCTCCAGTCA CAGCCCCCAG CCCCGTCCCC 
GGCACCCCGA CTCGTAAAAT CTCAGCTTCG GAATTTGACC GCCCGCTCAG ACCCATTGTG 
GTCAAAGACG CCGAGGGTTC TTTGACGTTC CTAAGCGACG CCGACCGCGA GACTGTCCCC 
GTGCGGGCTG CAGCGATGGG CGAGGGCGGA GGAGTCCACG TCGGGGGTTG CGACGAGGCA 
GGTAGTTGGT GCGGGCGCGA TCGCTGCGCG AGGCTGCTGG AGCTGGTGAA GGAAGTGTCG 
AGTCATCTGG ATGTGACAGC ACTCTGCCAC AAGATCTTCC TCCACATCAA CGAGCTGATC 
GCTGCAGACC GCTACTCGCT CTTCCTGGTC GGCGAGGACA GCTCCAATCG AAAGTTTTTG 
GTCAGCCGCC TGTTTGATGT GGCCGAGGGA TCCACGGTGG AGGAGTCCTC CAGTAGCTGC 
ATCCGGCTGG AGTGGAACAA AGGCATTGTG GGACATGTAG CTGCCACAGG ACAGCCGCTC 
AACATCAAGA ACGCTTACGA GGACTCCCGC TTTAACGCCG AGGTGGACCT GATCACGGGT 
TACAAAACCC ACAGCATCCT GTGTCTGCCA ATCAAGAACC ACCGAGACGA GGTGGTCGGA 
GTAGCTCAGG CCATCAATAA AAAAAGTGGA GATGACGCTG CCTTCACTGA CCAGGACGAA 
AAGGATTTCT CTGCCTATTT GGCCTTTTCC GGCATCGTCC TCCACAACGC TCAGCTCTAC 
GAGACGTCCC AGCTGGAAAA CAGACGTAAC CAGGTGCTGT TGGACCTCGC CAGCCTCATC 
TTCGAGGAGC AGCAGTGCCT GAAGGTTTTA CTCCGGAAGT TTGCGGGAAC CATCTTGTCC 
TTCATGCAGG CCCAGGCGTG CACGGTCTTC ATCGCCGACG AGGACTCTAT GAACTCCTTC 
TCCAGTGTCT TTCACATGGA ATACGAGGAG TTGGGGGAAG TCCTGGATGT CCCAAAAAGG 
GACTGTGATG TCAGCCAGAT CAACTACATG TATGCTCAGT ATGTCAAGAA CACCATGCAG 
ACGCTCAACA TTGCAGATGT GACCAAGGAC CAGCGCTTCC CCTGGACAGG GGAGAATCCA 
GATCACACCA ATGACCAGAT CAAGAGTTTG CTCTGCACTC CCATCAGGAA CGGAAAGAAG 
GACAAAGTGA TCGGTGTGTG TCAGCTGGTG AACAAGATGG ACGAAGCCTC GGACAGTGTG 
AAGGCTTTCA ACAGGAACGA TGAGCAATTC CTGGAGGCTT TTGCCATCTT CTGCGGCCTC 
GGCATCCAGA ACACACAGAT GTACGAGACG GTGGAGAGAG CCATGGCCAA ACAGGAAGTC 
ACCCTGGAGG TCTTGTCATA TCACGCCACT GCCTCCGAGG AAGAATCGCG GGAACTCCAG 
GTCGCCACGG TGCCGTCCGC TCAGTCACTG CGGCTTCTGG ACTTCAGCTT TAGCGACTTC 
GAGTTGTCCG ACACGGAAAC CACGCTGGCC ACCATCCGCA TGTTCGTCGA CCTCAACCTG 
GTCCAGAACT TCCAGATGAA GTACATGAAT TTATGTCGAT GGGTTCTGAG TGTGAAGAAG 
AACTACAGGA AGAGCGTGGC GTACCACAAC TGGAGACACG CCTTCAACAC GGCTCAGAGC 
ATGTTTGCTC TCCTCAAGTC TGGACGCTTG CAGAACAACC TGAACGACCT GGAGGTCTTA 
GCTCTGATGA TTGCCACTCT GAGCCACGAC CTGGACCACA GAGGAGTCAA CAACTCCTAC 
ATACAGAGGA GTGACCACCC ATTAGCCCAG CTCTACTGCC ACTCCACCAT GGAGCATCAC 
CACTTCGACC ACTGCCTCAT GATCCTCAAC AGCCCTGGAA ACCAGATCCT GAGCAGCCTG 
TCCCTGGACG AGTACAAATC CACTCTGAAA ATGATCGAGA GGGCTATTCT GGCCACGGAC 
CTGGCCCTGT ACATGAAGCG GCGTGGGGAG TTCTTTGAGC TCACCAAGAA CAGTCAGTTT 
GTCTGGGAAG ATGAGCAGCA CAGAGACTTA CTGAGGTCAA TGCTGATGAC TGCATGTGAC 
ATCTCAGCCA TCACCAAACC CTGGCCCGTG CAACAAAGGA TTGCAGAACT GGTGGCGACC 
GAGTTCTTCG AGCAGGGAGA TAAGGAAAGA CGCGAGCTGA ACATTGAACC CAGCGACCTG 
ATGAATCGAG AGAAAAAAGA CAAGATCCCC AGCATGCAGG TGTCCTTCAT TGACGCCATC 
TGTACCCAGT TATACGAGAC ATTGGCCGGC ATGTCGGAGT ACTGCTCCCC ACTTTTGGAG 
GGCTGTCAGA AAAATCGACA GCAGTGGAAG CATTTGGCCG AAGAGTGCGA GAAAGGATTG 
GTCAACGGCT TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011604779.1
CDS	97..2754
Translation	MEDCCYPESERMEAWLDDHLEFTRSYFIRKASREMVNAWFAERVHSIHPSAPKENPPQHPRTQQSSADSAVSSASAVPPPTITLPDYCCPSPVTAPSPVPGTPTRKISASEFDRPLRPIVVKDAEGSLTFLSDADRETVPVRAAAMGEGGGVHVGGCDEAGSWCGRDRCARLLELVKEVSSHLDVTALCHKIFLHINELIAADRYSLFLVGEDSSNRKFLVSRLFDVAEGSTVEESSSSCIRLEWNKGIVGHVAATGQPLNIKNAYEDSRFNAEVDLITGYKTHSILCLPIKNHRDEVVGVAQAINKKSGDDAAFTDQDEKDFSAYLAFSGIVLHNAQLYETSQLENRRNQVLLDLASLIFEEQQCLKVLLRKFAGTILSFMQAQACTVFIADEDSMNSFSSVFHMEYEELGEVLDVPKRDCDVSQINYMYAQYVKNTMQTLNIADVTKDQRFPWTGENPDHTNDQIKSLLCTPIRNGKKDKVIGVCQLVNKMDEASDSVKAFNRNDEQFLEAFAIFCGLGIQNTQMYETVERAMAKQEVTLEVLSYHATASEEESRELQVATVPSAQSLRLLDFSFSDFELSDTETTLATIRMFVDLNLVQNFQMKYMNLCRWVLSVKKNYRKSVAYHNWRHAFNTAQSMFALLKSGRLQNNLNDLEVLALMIATLSHDLDHRGVNNSYIQRSDHPLAQLYCHSTMEHHHFDHCLMILNSPGNQILSSLSLDEYKSTLKMIERAILATDLALYMKRRGEFFELTKNSQFVWEDEQHRDLLRSMLMTACDISAITKPWPVQQRIAELVATEFFEQGDKERRELNIEPSDLMNREKKDKIPSMQVSFIDAICTQLYETLAGMSEYCSPLLEGCQKNRQQWKHLAEECEKGLVNGLV

Target ORF information:

RefSeq Version	XM_011606477.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes phosphodiesterase 5A, cGMP-specific (pde5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011606477.1

ORF Insert Sequence:

ATGGAGGACT GTTGCTACCC GGAAAGCGAG AGGATGGAAG CGTGGCTGGA CGACCACTTG 
GAGTTCACGC GCTCCTATTT TATCAGGAAG GCGTCAAGGG AGATGGTCAA CGCCTGGTTC 
GCTGAGCGAG TCCACTCCAT CCATCCTTCG GCTCCTAAAG AGAATCCTCC TCAGCATCCC 
AGGACCCAGC AGTCCTCTGC AGACTCTGCT GTCTCCTCAG CCTCCGCCGT CCCCCCGCCC 
ACCATCACCC TGCCGGACTA CTGCTGCCCC TCTCCAGTCA CAGCCCCCAG CCCCGTCCCC 
GGCACCCCGA CTCGTAAAAT CTCAGCTTCG GAATTTGACC GCCCGCTCAG ACCCATTGTG 
GTCAAAGACG CCGAGGGTTC TTTGACGTTC CTAAGCGACG CCGACCGCGA GACTGTCCCC 
GTGCGGGCTG CAGCGATGGG CGAGGGCGGA GGAGTCCACG TCGGGGGTTG CGACGAGGCA 
GGTAGTTGGT GCGGGCGCGA TCGCTGCGCG AGGCTGCTGG AGCTGGTGAA GGAAGTGTCG 
AGTCATCTGG ATGTGACAGC ACTCTGCCAC AAGATCTTCC TCCACATCAA CGAGCTGATC 
GCTGCAGACC GCTACTCGCT CTTCCTGGTC GGCGAGGACA GCTCCAATCG AAAGTTTTTG 
GTCAGCCGCC TGTTTGATGT GGCCGAGGGA TCCACGGTGG AGGAGTCCTC CAGTAGCTGC 
ATCCGGCTGG AGTGGAACAA AGGCATTGTG GGACATGTAG CTGCCACAGG ACAGCCGCTC 
AACATCAAGA ACGCTTACGA GGACTCCCGC TTTAACGCCG AGGTGGACCT GATCACGGGT 
TACAAAACCC ACAGCATCCT GTGTCTGCCA ATCAAGAACC ACCGAGACGA GGTGGTCGGA 
GTAGCTCAGG CCATCAATAA AAAAAGTGGA GATGACGCTG CCTTCACTGA CCAGGACGAA 
AAGGATTTCT CTGCCTATTT GGCCTTTTCC GGCATCGTCC TCCACAACGC TCAGCTCTAC 
GAGACGTCCC AGCTGGAAAA CAGACGTAAC CAGGTGCTGT TGGACCTCGC CAGCCTCATC 
TTCGAGGAGC AGCAGTGCCT GAAGGTTTTA CTCCGGAAGT TTGCGGGAAC CATCTTGTCC 
TTCATGCAGG CCCAGGCGTG CACGGTCTTC ATCGCCGACG AGGACTCTAT GAACTCCTTC 
TCCAGTGTCT TTCACATGGA ATACGAGGAG TTGGGGGAAG TCCTGGATGT CCCAAAAAGG 
GACTGTGATG TCAGCCAGAT CAACTACATG TATGCTCAGT ATGTCAAGAA CACCATGCAG 
ACGCTCAACA TTGCAGATGT GACCAAGGAC CAGCGCTTCC CCTGGACAGG GGAGAATCCA 
GATCACACCA ATGACCAGAT CAAGAGTTTG CTCTGCACTC CCATCAGGAA CGGAAAGAAG 
GACAAAGTGA TCGGTGTGTG TCAGCTGGTG AACAAGATGG ACGAAGCCTC GGACAGTGTG 
AAGGCTTTCA ACAGGAACGA TGAGCAATTC CTGGAGGCTT TTGCCATCTT CTGCGGCCTC 
GGCATCCAGA ACACACAGAT GTACGAGACG GTGGAGAGAG CCATGGCCAA ACAGGAAGTC 
ACCCTGGAGG TCTTGTCATA TCACGCCACT GCCTCCGAGG AAGAATCGCG GGAACTCCAG 
GTCGCCACGG TGCCGTCCGC TCAGTCACTG CGGCTTCTGG ACTTCAGCTT TAGCGACTTC 
GAGTTGTCCG ACACGGAAAC CACGCTGGCC ACCATCCGCA TGTTCGTCGA CCTCAACCTG 
GTCCAGAACT TCCAGATGAA GTACATGAAT TTATGTCGAT GGGTTCTGAG TGTGAAGAAG 
AACTACAGGA AGAGCGTGGC GTACCACAAC TGGAGACACG CCTTCAACAC GGCTCAGAGC 
ATGTTTGCTC TCCTCAAGTC TGGACGCTTG CAGAACAACC TGAACGACCT GGAGGTCTTA 
GCTCTGATGA TTGCCACTCT GAGCCACGAC CTGGACCACA GAGGAGTCAA CAACTCCTAC 
ATACAGAGGA GTGACCACCC ATTAGCCCAG CTCTACTGCC ACTCCACCAT GGAGCATCAC 
CACTTCGACC ACTGCCTCAT GATCCTCAAC AGCCCTGGAA ACCAGATCCT GAGCAGCCTG 
TCCCTGGACG AGTACAAATC CACTCTGAAA ATGATCGAGA GGGCTATTCT GGCCACGGAC 
CTGGCCCTGT ACATGAAGCG GCGTGGGGAG TTCTTTGAGC TCACCAAGAA CAGTCAGTTT 
GTCTGGGAAG ATGAGCAGCA CAGAGACTTA CTGAGGTCAA TGCTGATGAC TGCATGTGAC 
ATCTCAGCCA TCACCAAACC CTGGCCCGTG CAACAAAGGA TTGCAGAACT GGTGGCGACC 
GAGTTCTTCG AGCAGGGAGA TAAGGAAAGA CGCGAGCTGA ACATTGAACC CAGCGACCTG 
ATGAATCGAG AGAAAAAAGA CAAGATCCCC AGCATGCAGG TGTCCTTCAT TGACGCCATC 
TGTACCCAGT TATACGAGAC ATTGGCCGGC ATGTCGGAGT ACTGCTCCCC ACTTTTGGAG 
GGCTGTCAGA AAAATCGACA GCAGTGGAAG CATTTGGCCG AAGAGTGCGA GAAAGGATTG 
GTCAACGGCT TGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde5a ( XM_029840309.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696169.1 Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X1, mRNA.

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CloneID	OTo71195
Clone ID Related Accession (Same CDS sequence)	XM_029840309.1
Accession Version	XM_029840309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2673bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACCC AACTGACTCA TTATCTCCCC GAGACACAAA AACAGCCACT ACAGAAGATG 
GACGACGGGT GTTTCATGTC CAACCAGCAC CGTCCGGTCC AGAGGGAGAT GGTCAACGCC 
TGGTTCGCTG AGCGAGTCCA CTCCATCCAT CCATCGGCTC CTAAAGAGAA TCCTCCTCAG 
CATCCCAGGA CCCAGCAGTC CTCTGCAGAC TCTGCTGTCT CCTCAGCCTC CGCCGTCCCC 
CCGCCCACCA TCACCCTGCC GGACTACTGC TGCCCCTCTC CAGTCACAGC CCCCAGCCCC 
GTCCCCGGCA CCCCGACTCG TAAAATCTCA GCTTCGGAAT TTGACCGCCC GCTCAGACCC 
ATTGTGGTCA AAGACGCCGA GGGTTCTTTG ACGTTCCTAA GCGACGCCGA CCGCGAGACT 
GTCCCCGTGC GGGCTGCAGC GATGGGCGAG GGCGGAGGAG TCCACGTCGG GGGTTGCGAC 
GAGGCAGGTA GTTGGTGCGG GCGCGATCGC TGCGCGAGGC TGCTGGAGCT GGTGAAGGAA 
GTGTCGAGTC ATCTGGATGT GACAGCACTC TGCCACAAGA TCTTCCTCCA CATCAACGAG 
CTGATCGCTG CAGACCGCTA CTCGCTCTTC CTGGTCGGCG AGGACAGCTC CAATCGAAAG 
TTTTTGGTCA GCCGCCTGTT TGATGTGGCC GAGGGATCCA CGGTGGAGGA GTCCTCCAGT 
AGCTGCATCC GGCTGGAGTG GAACAAAGGC ATTGTGGGAC ATGTAGCTGC CACAGGACAG 
CCGCTCAACA TCAAGAACGC TTACGAGGAC TCCCGCTTTA ACGCCGAGGT GGACCTGATC 
ACGGGTTACA AAACCCACAG CATCCTGTGT CTGCCAATCA AGAACCACCG AGACGAGGTG 
GTCGGAGTAG CTCAGGCCAT CAATAAAAAA AGTGGAGATG ACGCTGCCTT CACTGACCAG 
GACGAAAAGG ATTTCTCCGC CTATTTGGCC TTTTCCGGCA TCGTCCTCCA CAACGCTCAG 
CTCTACGAGA CGTCCCAGCT GGAAAACAGA CGTAACCAGG TGCTGTTGGA CCTCGCCAGC 
CTCATCTTCG AGGAGCAGCA GTGCCTGAAG GTTTTACTCC GGAAGTTTGC GGGAACCATC 
TTGTCCTTCA TGCAGGCCCA GGCGTGCACG GTCTTCATCG CCGACGAGGA CTCTATGAAC 
TCCTTCTCCA GTGTCTTTCA CATGGAATAC GAGGAGTTGG GGGAAGTCCT GGATGTCCCA 
AAAAGGGACT GTGATGTCAG CCAGATCAAC TACATGTATG CTCAGTATGT CAAGAACACC 
ATGCAGACGC TCAACATTGC AGACGTGACC AAGGACCAGC GCTTCCCCTG GACAGGGGAG 
AATCCAGATC ACACCAATGA CCAGATCAAG AGTTTGCTCT GCACTCCCAT CAGGAACGGA 
AAGAAGGACA AAGTGATCGG TGTGTGTCAG CTGGTGAACA AGATGGACGA AGCCTCGGAC 
AGTGTGAAGG CTTTCAACAG GAACGATGAG CAATTCCTGG AGGCTTTTGC CATCTTCTGC 
GGCCTCGGCA TCCAGAACAC ACAGATGTAC GAGACGGTGG AGAGAGCCAT GGCCAAACAG 
GAAGTCACCC TGGAGGTCTT GTCATATCAC GCCACTGCCT CCGAGGAAGA ATCGCGGGAA 
CTCCAGGTAA CCGTGGTCGC CACGGTGCCG TCCGCTCAGT CACTGCGGCT TCTGGACTTC 
AGCTTTAGCG ACTTCGAGTT GTCCGACACG GAAACCACGC TGGCCACCAT CCGCATGTTC 
GTCGACCTCA ACCTGGTCCA GAACTTCCAG ATGAAGTACA TGAATTTATG TCGATGGGTT 
CTGAGTGTGA AGAAGAACTA CAGGAAGAGC GTGGCGTACC ACAACTGGAG ACACGCCTTC 
AACACGGCTC AGAGCATGTT TGCTCTCCTC AAGTCTGGAC GCTTGCAGAA CAACCTGAAC 
GACCTGGAGG TCTTAGCTCT GATGATTGCC ACTCTGAGCC ACGACCTGGA CCACAGAGGA 
GTCAACAACT CCTACATACA GAGGAGTGAC CACCCATTAG CCCAGCTCTA CTGCCACTCC 
ACCATGGAGC ATCACCACTT CGACCACTGC CTCATGATCC TCAACAGCCC TGGAAACCAG 
ATCCTGAGCA GCCTGTCCCT GGACGAGTAC AAATCCACTC TGAAAATGAT CGAGAGGGCT 
ATTCTGGCCA CGGACCTGGC CCTGTACATG AAGCGGCGTG GGGAGTTCTT TGAGCTCACC 
AAGAACAGTC AGTTTGTCTG GGAAGATGAG CAGCACAGAG ACTTACTGAG GTCAATGCTG 
ATGACTGCAT GTGACATCTC AGCCATCACC AAACCCTGGC CCGTGCAACA AAGGATTGCA 
GAACTGGTGG CGACCGAGTT CTTCGAGCAG GGAGATAAGG AAAGACGCGA GCTGAACATT 
GAACCCAGCG ACCTGATGAA TCGAGAGAAA AAAGACAAGA TCCCCAGCAT GCAGGTGTCC 
TTCATTGACG CCATCTGTAC CCAGTTATAC GAGACATTGG CCGGCATGTC GGAGTACTGC 
TCCCCACTTT TGGAGGGCTG TCAGAAAAAT CGACAGCAGT GGAAGCATTT GGCCGAAGAG 
TGCGAGAAAG GATTGGTCAA CGGCTTGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696169.1
CDS	182..2854
Translation	MTTQLTHYLPETQKQPLQKMDDGCFMSNQHRPVQREMVNAWFAERVHSIHPSAPKENPPQHPRTQQSSADSAVSSASAVPPPTITLPDYCCPSPVTAPSPVPGTPTRKISASEFDRPLRPIVVKDAEGSLTFLSDADRETVPVRAAAMGEGGGVHVGGCDEAGSWCGRDRCARLLELVKEVSSHLDVTALCHKIFLHINELIAADRYSLFLVGEDSSNRKFLVSRLFDVAEGSTVEESSSSCIRLEWNKGIVGHVAATGQPLNIKNAYEDSRFNAEVDLITGYKTHSILCLPIKNHRDEVVGVAQAINKKSGDDAAFTDQDEKDFSAYLAFSGIVLHNAQLYETSQLENRRNQVLLDLASLIFEEQQCLKVLLRKFAGTILSFMQAQACTVFIADEDSMNSFSSVFHMEYEELGEVLDVPKRDCDVSQINYMYAQYVKNTMQTLNIADVTKDQRFPWTGENPDHTNDQIKSLLCTPIRNGKKDKVIGVCQLVNKMDEASDSVKAFNRNDEQFLEAFAIFCGLGIQNTQMYETVERAMAKQEVTLEVLSYHATASEEESRELQVTVVATVPSAQSLRLLDFSFSDFELSDTETTLATIRMFVDLNLVQNFQMKYMNLCRWVLSVKKNYRKSVAYHNWRHAFNTAQSMFALLKSGRLQNNLNDLEVLALMIATLSHDLDHRGVNNSYIQRSDHPLAQLYCHSTMEHHHFDHCLMILNSPGNQILSSLSLDEYKSTLKMIERAILATDLALYMKRRGEFFELTKNSQFVWEDEQHRDLLRSMLMTACDISAITKPWPVQQRIAELVATEFFEQGDKERRELNIEPSDLMNREKKDKIPSMQVSFIDAICTQLYETLAGMSEYCSPLLEGCQKNRQQWKHLAEECEKGLVNGLV

Target ORF information:

RefSeq Version	XM_029840309.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840309.1

ORF Insert Sequence:

ATGACAACCC AACTGACTCA TTATCTCCCC GAGACACAAA AACAGCCACT ACAGAAGATG 
GACGACGGGT GTTTCATGTC CAACCAGCAC CGTCCGGTCC AGAGGGAGAT GGTCAACGCC 
TGGTTCGCTG AGCGAGTCCA CTCCATCCAT CCATCGGCTC CTAAAGAGAA TCCTCCTCAG 
CATCCCAGGA CCCAGCAGTC CTCTGCAGAC TCTGCTGTCT CCTCAGCCTC CGCCGTCCCC 
CCGCCCACCA TCACCCTGCC GGACTACTGC TGCCCCTCTC CAGTCACAGC CCCCAGCCCC 
GTCCCCGGCA CCCCGACTCG TAAAATCTCA GCTTCGGAAT TTGACCGCCC GCTCAGACCC 
ATTGTGGTCA AAGACGCCGA GGGTTCTTTG ACGTTCCTAA GCGACGCCGA CCGCGAGACT 
GTCCCCGTGC GGGCTGCAGC GATGGGCGAG GGCGGAGGAG TCCACGTCGG GGGTTGCGAC 
GAGGCAGGTA GTTGGTGCGG GCGCGATCGC TGCGCGAGGC TGCTGGAGCT GGTGAAGGAA 
GTGTCGAGTC ATCTGGATGT GACAGCACTC TGCCACAAGA TCTTCCTCCA CATCAACGAG 
CTGATCGCTG CAGACCGCTA CTCGCTCTTC CTGGTCGGCG AGGACAGCTC CAATCGAAAG 
TTTTTGGTCA GCCGCCTGTT TGATGTGGCC GAGGGATCCA CGGTGGAGGA GTCCTCCAGT 
AGCTGCATCC GGCTGGAGTG GAACAAAGGC ATTGTGGGAC ATGTAGCTGC CACAGGACAG 
CCGCTCAACA TCAAGAACGC TTACGAGGAC TCCCGCTTTA ACGCCGAGGT GGACCTGATC 
ACGGGTTACA AAACCCACAG CATCCTGTGT CTGCCAATCA AGAACCACCG AGACGAGGTG 
GTCGGAGTAG CTCAGGCCAT CAATAAAAAA AGTGGAGATG ACGCTGCCTT CACTGACCAG 
GACGAAAAGG ATTTCTCCGC CTATTTGGCC TTTTCCGGCA TCGTCCTCCA CAACGCTCAG 
CTCTACGAGA CGTCCCAGCT GGAAAACAGA CGTAACCAGG TGCTGTTGGA CCTCGCCAGC 
CTCATCTTCG AGGAGCAGCA GTGCCTGAAG GTTTTACTCC GGAAGTTTGC GGGAACCATC 
TTGTCCTTCA TGCAGGCCCA GGCGTGCACG GTCTTCATCG CCGACGAGGA CTCTATGAAC 
TCCTTCTCCA GTGTCTTTCA CATGGAATAC GAGGAGTTGG GGGAAGTCCT GGATGTCCCA 
AAAAGGGACT GTGATGTCAG CCAGATCAAC TACATGTATG CTCAGTATGT CAAGAACACC 
ATGCAGACGC TCAACATTGC AGACGTGACC AAGGACCAGC GCTTCCCCTG GACAGGGGAG 
AATCCAGATC ACACCAATGA CCAGATCAAG AGTTTGCTCT GCACTCCCAT CAGGAACGGA 
AAGAAGGACA AAGTGATCGG TGTGTGTCAG CTGGTGAACA AGATGGACGA AGCCTCGGAC 
AGTGTGAAGG CTTTCAACAG GAACGATGAG CAATTCCTGG AGGCTTTTGC CATCTTCTGC 
GGCCTCGGCA TCCAGAACAC ACAGATGTAC GAGACGGTGG AGAGAGCCAT GGCCAAACAG 
GAAGTCACCC TGGAGGTCTT GTCATATCAC GCCACTGCCT CCGAGGAAGA ATCGCGGGAA 
CTCCAGGTAA CCGTGGTCGC CACGGTGCCG TCCGCTCAGT CACTGCGGCT TCTGGACTTC 
AGCTTTAGCG ACTTCGAGTT GTCCGACACG GAAACCACGC TGGCCACCAT CCGCATGTTC 
GTCGACCTCA ACCTGGTCCA GAACTTCCAG ATGAAGTACA TGAATTTATG TCGATGGGTT 
CTGAGTGTGA AGAAGAACTA CAGGAAGAGC GTGGCGTACC ACAACTGGAG ACACGCCTTC 
AACACGGCTC AGAGCATGTT TGCTCTCCTC AAGTCTGGAC GCTTGCAGAA CAACCTGAAC 
GACCTGGAGG TCTTAGCTCT GATGATTGCC ACTCTGAGCC ACGACCTGGA CCACAGAGGA 
GTCAACAACT CCTACATACA GAGGAGTGAC CACCCATTAG CCCAGCTCTA CTGCCACTCC 
ACCATGGAGC ATCACCACTT CGACCACTGC CTCATGATCC TCAACAGCCC TGGAAACCAG 
ATCCTGAGCA GCCTGTCCCT GGACGAGTAC AAATCCACTC TGAAAATGAT CGAGAGGGCT 
ATTCTGGCCA CGGACCTGGC CCTGTACATG AAGCGGCGTG GGGAGTTCTT TGAGCTCACC 
AAGAACAGTC AGTTTGTCTG GGAAGATGAG CAGCACAGAG ACTTACTGAG GTCAATGCTG 
ATGACTGCAT GTGACATCTC AGCCATCACC AAACCCTGGC CCGTGCAACA AAGGATTGCA 
GAACTGGTGG CGACCGAGTT CTTCGAGCAG GGAGATAAGG AAAGACGCGA GCTGAACATT 
GAACCCAGCG ACCTGATGAA TCGAGAGAAA AAAGACAAGA TCCCCAGCAT GCAGGTGTCC 
TTCATTGACG CCATCTGTAC CCAGTTATAC GAGACATTGG CCGGCATGTC GGAGTACTGC 
TCCCCACTTT TGGAGGGCTG TCAGAAAAAT CGACAGCAGT GGAAGCATTT GGCCGAAGAG 
TGCGAGAAAG GATTGGTCAA CGGCTTGGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde5a ( XM_003966740.3 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_003966789.1 Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo71196
Clone ID Related Accession (Same CDS sequence)	XM_003966740.3
Accession Version	XM_003966740.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_003966740.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACT GTTGCTACCC GGAAAGCGAG AGGATGGAAG CGTGGCTGGA CGACCACTTG 
GAGTTCACGC GCTCCTATTT TATCAGGAAG GCGTCAAGGG AGATGGTCAA CGCCTGGTTC 
GCTGAGCGAG TCCACTCCAT CCATCCATCG GCTCCTAAAG AGAATCCTCC TCAGCATCCC 
AGGACCCAGC AGTCCTCTGC AGACTCTGCT GTCTCCTCAG CCTCCGCCGT CCCCCCGCCC 
ACCATCACCC TGCCGGACTA CTGCTGCCCC TCTCCAGTCA CAGCCCCCAG CCCCGTCCCC 
GGCACCCCGA CTCGTAAAAT CTCAGCTTCG GAATTTGACC GCCCGCTCAG ACCCATTGTG 
GTCAAAGACG CCGAGGGTTC TTTGACGTTC CTAAGCGACG CCGACCGCGA GACTGTCCCC 
GTGCGGGCTG CAGCGATGGG CGAGGGCGGA GGAGTCCACG TCGGGGGTTG CGACGAGGCA 
GGTAGTTGGT GCGGGCGCGA TCGCTGCGCG AGGCTGCTGG AGCTGGTGAA GGAAGTGTCG 
AGTCATCTGG ATGTGACAGC ACTCTGCCAC AAGATCTTCC TCCACATCAA CGAGCTGATC 
GCTGCAGACC GCTACTCGCT CTTCCTGGTC GGCGAGGACA GCTCCAATCG AAAGTTTTTG 
GTCAGCCGCC TGTTTGATGT GGCCGAGGGA TCCACGGTGG AGGAGTCCTC CAGTAGCTGC 
ATCCGGCTGG AGTGGAACAA AGGCATTGTG GGACATGTAG CTGCCACAGG ACAGCCGCTC 
AACATCAAGA ACGCTTACGA GGACTCCCGC TTTAACGCCG AGGTGGACCT GATCACGGGT 
TACAAAACCC ACAGCATCCT GTGTCTGCCA ATCAAGAACC ACCGAGACGA GGTGGTCGGA 
GTAGCTCAGG CCATCAATAA AAAAAGTGGA GATGACGCTG CCTTCACTGA CCAGGACGAA 
AAGGATTTCT CCGCCTATTT GGCCTTTTCC GGCATCGTCC TCCACAACGC TCAGCTCTAC 
GAGACGTCCC AGCTGGAAAA CAGACGTAAC CAGGTGCTGT TGGACCTCGC CAGCCTCATC 
TTCGAGGAGC AGCAGTGCCT GAAGGTTTTA CTCCGGAAGT TTGCGGGAAC CATCTTGTCC 
TTCATGCAGG CCCAGGCGTG CACGGTCTTC ATCGCCGACG AGGACTCTAT GAACTCCTTC 
TCCAGTGTCT TTCACATGGA ATACGAGGAG TTGGGGGAAG TCCTGGATGT CCCAAAAAGG 
GACTGTGATG TCAGCCAGAT CAACTACATG TATGCTCAGT ATGTCAAGAA CACCATGCAG 
ACGCTCAACA TTGCAGACGT GACCAAGGAC CAGCGCTTCC CCTGGACAGG GGAGAATCCA 
GATCACACCA ATGACCAGAT CAAGAGTTTG CTCTGCACTC CCATCAGGAA CGGAAAGAAG 
GACAAAGTGA TCGGTGTGTG TCAGCTGGTG AACAAGATGG ACGAAGCCTC GGACAGTGTG 
AAGGCTTTCA ACAGGAACGA TGAGCAATTC CTGGAGGCTT TTGCCATCTT CTGCGGCCTC 
GGCATCCAGA ACACACAGAT GTACGAGACG GTGGAGAGAG CCATGGCCAA ACAGGAAGTC 
ACCCTGGAGG TCTTGTCATA TCACGCCACT GCCTCCGAGG AAGAATCGCG GGAACTCCAG 
GTAACCGTGG TCGCCACGGT GCCGTCCGCT CAGTCACTGC GGCTTCTGGA CTTCAGCTTT 
AGCGACTTCG AGTTGTCCGA CACGGAAACC ACGCTGGCCA CCATCCGCAT GTTCGTCGAC 
CTCAACCTGG TCCAGAACTT CCAGATGAAG TACATGAATT TATGTCGATG GGTTCTGAGT 
GTGAAGAAGA ACTACAGGAA GAGCGTGGCG TACCACAACT GGAGACACGC CTTCAACACG 
GCTCAGAGCA TGTTTGCTCT CCTCAAGTCT GGACGCTTGC AGAACAACCT GAACGACCTG 
GAGGTCTTAG CTCTGATGAT TGCCACTCTG AGCCACGACC TGGACCACAG AGGAGTCAAC 
AACTCCTACA TACAGAGGAG TGACCACCCA TTAGCCCAGC TCTACTGCCA CTCCACCATG 
GAGCATCACC ACTTCGACCA CTGCCTCATG ATCCTCAACA GCCCTGGAAA CCAGATCCTG 
AGCAGCCTGT CCCTGGACGA GTACAAATCC ACTCTGAAAA TGATCGAGAG GGCTATTCTG 
GCCACGGACC TGGCCCTGTA CATGAAGCGG CGTGGGGAGT TCTTTGAGCT CACCAAGAAC 
AGTCAGTTTG TCTGGGAAGA TGAGCAGCAC AGAGACTTAC TGAGGTCAAT GCTGATGACT 
GCATGTGACA TCTCAGCCAT CACCAAACCC TGGCCCGTGC AACAAAGGAT TGCAGAACTG 
GTGGCGACCG AGTTCTTCGA GCAGGGAGAT AAGGAAAGAC GCGAGCTGAA CATTGAACCC 
AGCGACCTGA TGAATCGAGA GAAAAAAGAC AAGATCCCCA GCATGCAGGT GTCCTTCATT 
GACGCCATCT GTACCCAGTT ATACGAGACA TTGGCCGGCA TGTCGGAGTA CTGCTCCCCA 
CTTTTGGAGG GCTGTCAGAA AAATCGACAG CAGTGGAAGC ATTTGGCCGA AGAGTGCGAG 
AAAGGATTGG TCAACGGCTT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003966789.1
CDS	1529..4195
Translation	MEDCCYPESERMEAWLDDHLEFTRSYFIRKASREMVNAWFAERVHSIHPSAPKENPPQHPRTQQSSADSAVSSASAVPPPTITLPDYCCPSPVTAPSPVPGTPTRKISASEFDRPLRPIVVKDAEGSLTFLSDADRETVPVRAAAMGEGGGVHVGGCDEAGSWCGRDRCARLLELVKEVSSHLDVTALCHKIFLHINELIAADRYSLFLVGEDSSNRKFLVSRLFDVAEGSTVEESSSSCIRLEWNKGIVGHVAATGQPLNIKNAYEDSRFNAEVDLITGYKTHSILCLPIKNHRDEVVGVAQAINKKSGDDAAFTDQDEKDFSAYLAFSGIVLHNAQLYETSQLENRRNQVLLDLASLIFEEQQCLKVLLRKFAGTILSFMQAQACTVFIADEDSMNSFSSVFHMEYEELGEVLDVPKRDCDVSQINYMYAQYVKNTMQTLNIADVTKDQRFPWTGENPDHTNDQIKSLLCTPIRNGKKDKVIGVCQLVNKMDEASDSVKAFNRNDEQFLEAFAIFCGLGIQNTQMYETVERAMAKQEVTLEVLSYHATASEEESRELQVTVVATVPSAQSLRLLDFSFSDFELSDTETTLATIRMFVDLNLVQNFQMKYMNLCRWVLSVKKNYRKSVAYHNWRHAFNTAQSMFALLKSGRLQNNLNDLEVLALMIATLSHDLDHRGVNNSYIQRSDHPLAQLYCHSTMEHHHFDHCLMILNSPGNQILSSLSLDEYKSTLKMIERAILATDLALYMKRRGEFFELTKNSQFVWEDEQHRDLLRSMLMTACDISAITKPWPVQQRIAELVATEFFEQGDKERRELNIEPSDLMNREKKDKIPSMQVSFIDAICTQLYETLAGMSEYCSPLLEGCQKNRQQWKHLAEECEKGLVNGLV

Target ORF information:

RefSeq Version	XM_003966740.3
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003966740.3

ORF Insert Sequence:

ATGGAGGACT GTTGCTACCC GGAAAGCGAG AGGATGGAAG CGTGGCTGGA CGACCACTTG 
GAGTTCACGC GCTCCTATTT TATCAGGAAG GCGTCAAGGG AGATGGTCAA CGCCTGGTTC 
GCTGAGCGAG TCCACTCCAT CCATCCATCG GCTCCTAAAG AGAATCCTCC TCAGCATCCC 
AGGACCCAGC AGTCCTCTGC AGACTCTGCT GTCTCCTCAG CCTCCGCCGT CCCCCCGCCC 
ACCATCACCC TGCCGGACTA CTGCTGCCCC TCTCCAGTCA CAGCCCCCAG CCCCGTCCCC 
GGCACCCCGA CTCGTAAAAT CTCAGCTTCG GAATTTGACC GCCCGCTCAG ACCCATTGTG 
GTCAAAGACG CCGAGGGTTC TTTGACGTTC CTAAGCGACG CCGACCGCGA GACTGTCCCC 
GTGCGGGCTG CAGCGATGGG CGAGGGCGGA GGAGTCCACG TCGGGGGTTG CGACGAGGCA 
GGTAGTTGGT GCGGGCGCGA TCGCTGCGCG AGGCTGCTGG AGCTGGTGAA GGAAGTGTCG 
AGTCATCTGG ATGTGACAGC ACTCTGCCAC AAGATCTTCC TCCACATCAA CGAGCTGATC 
GCTGCAGACC GCTACTCGCT CTTCCTGGTC GGCGAGGACA GCTCCAATCG AAAGTTTTTG 
GTCAGCCGCC TGTTTGATGT GGCCGAGGGA TCCACGGTGG AGGAGTCCTC CAGTAGCTGC 
ATCCGGCTGG AGTGGAACAA AGGCATTGTG GGACATGTAG CTGCCACAGG ACAGCCGCTC 
AACATCAAGA ACGCTTACGA GGACTCCCGC TTTAACGCCG AGGTGGACCT GATCACGGGT 
TACAAAACCC ACAGCATCCT GTGTCTGCCA ATCAAGAACC ACCGAGACGA GGTGGTCGGA 
GTAGCTCAGG CCATCAATAA AAAAAGTGGA GATGACGCTG CCTTCACTGA CCAGGACGAA 
AAGGATTTCT CCGCCTATTT GGCCTTTTCC GGCATCGTCC TCCACAACGC TCAGCTCTAC 
GAGACGTCCC AGCTGGAAAA CAGACGTAAC CAGGTGCTGT TGGACCTCGC CAGCCTCATC 
TTCGAGGAGC AGCAGTGCCT GAAGGTTTTA CTCCGGAAGT TTGCGGGAAC CATCTTGTCC 
TTCATGCAGG CCCAGGCGTG CACGGTCTTC ATCGCCGACG AGGACTCTAT GAACTCCTTC 
TCCAGTGTCT TTCACATGGA ATACGAGGAG TTGGGGGAAG TCCTGGATGT CCCAAAAAGG 
GACTGTGATG TCAGCCAGAT CAACTACATG TATGCTCAGT ATGTCAAGAA CACCATGCAG 
ACGCTCAACA TTGCAGACGT GACCAAGGAC CAGCGCTTCC CCTGGACAGG GGAGAATCCA 
GATCACACCA ATGACCAGAT CAAGAGTTTG CTCTGCACTC CCATCAGGAA CGGAAAGAAG 
GACAAAGTGA TCGGTGTGTG TCAGCTGGTG AACAAGATGG ACGAAGCCTC GGACAGTGTG 
AAGGCTTTCA ACAGGAACGA TGAGCAATTC CTGGAGGCTT TTGCCATCTT CTGCGGCCTC 
GGCATCCAGA ACACACAGAT GTACGAGACG GTGGAGAGAG CCATGGCCAA ACAGGAAGTC 
ACCCTGGAGG TCTTGTCATA TCACGCCACT GCCTCCGAGG AAGAATCGCG GGAACTCCAG 
GTAACCGTGG TCGCCACGGT GCCGTCCGCT CAGTCACTGC GGCTTCTGGA CTTCAGCTTT 
AGCGACTTCG AGTTGTCCGA CACGGAAACC ACGCTGGCCA CCATCCGCAT GTTCGTCGAC 
CTCAACCTGG TCCAGAACTT CCAGATGAAG TACATGAATT TATGTCGATG GGTTCTGAGT 
GTGAAGAAGA ACTACAGGAA GAGCGTGGCG TACCACAACT GGAGACACGC CTTCAACACG 
GCTCAGAGCA TGTTTGCTCT CCTCAAGTCT GGACGCTTGC AGAACAACCT GAACGACCTG 
GAGGTCTTAG CTCTGATGAT TGCCACTCTG AGCCACGACC TGGACCACAG AGGAGTCAAC 
AACTCCTACA TACAGAGGAG TGACCACCCA TTAGCCCAGC TCTACTGCCA CTCCACCATG 
GAGCATCACC ACTTCGACCA CTGCCTCATG ATCCTCAACA GCCCTGGAAA CCAGATCCTG 
AGCAGCCTGT CCCTGGACGA GTACAAATCC ACTCTGAAAA TGATCGAGAG GGCTATTCTG 
GCCACGGACC TGGCCCTGTA CATGAAGCGG CGTGGGGAGT TCTTTGAGCT CACCAAGAAC 
AGTCAGTTTG TCTGGGAAGA TGAGCAGCAC AGAGACTTAC TGAGGTCAAT GCTGATGACT 
GCATGTGACA TCTCAGCCAT CACCAAACCC TGGCCCGTGC AACAAAGGAT TGCAGAACTG 
GTGGCGACCG AGTTCTTCGA GCAGGGAGAT AAGGAAAGAC GCGAGCTGAA CATTGAACCC 
AGCGACCTGA TGAATCGAGA GAAAAAAGAC AAGATCCCCA GCATGCAGGT GTCCTTCATT 
GACGCCATCT GTACCCAGTT ATACGAGACA TTGGCCGGCA TGTCGGAGTA CTGCTCCCCA 
CTTTTGGAGG GCTGTCAGAA AAATCGACAG CAGTGGAAGC ATTTGGCCGA AGAGTGCGAG 
AAAGGATTGG TCAACGGCTT GGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde5a ( XM_029840310.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696170.1 Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo71197
Clone ID Related Accession (Same CDS sequence)	XM_029840310.1
Accession Version	XM_029840310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACCC AACTGACTCA TTATCTCCCC GAGACACAAA AACAGCCACT ACAGAAGATG 
GACGACGGGT GTTTCATGTC CAACCAGCAC CGTCCGGTCC AGAGGGAGAT GGTCAACGCC 
TGGTTCGCTG AGCGAGTCCA CTCCATCCAT CCATCGGCTC CTAAAGAGAA TCCTCCTCAG 
CATCCCAGGA CCCAGCAGTC CTCTGCAGAC TCTGCTGTCT CCTCAGCCTC CGCCGTCCCC 
CCGCCCACCA TCACCCTGCC GGACTACTGC TGCCCCTCTC CAGTCACAGC CCCCAGCCCC 
GTCCCCGGCA CCCCGACTCG TAAAATCTCA GCTTCGGAAT TTGACCGCCC GCTCAGACCC 
ATTGTGGTCA AAGACGCCGA GGGTTCTTTG ACGTTCCTAA GCGACGCCGA CCGCGAGACT 
GTCCCCGTGC GGGCTGCAGC GATGGGCGAG GGCGGAGGAG TCCACGTCGG GGGTTGCGAC 
GAGGCAGGTA GTTGGTGCGG GCGCGATCGC TGCGCGAGGC TGCTGGAGCT GGTGAAGGAA 
GTGTCGAGTC ATCTGGATGT GACAGCACTC TGCCACAAGA TCTTCCTCCA CATCAACGAG 
CTGATCGCTG CAGACCGCTA CTCGCTCTTC CTGGTCGGCG AGGACAGCTC CAATCGAAAG 
TTTTTGGTCA GCCGCCTGTT TGATGTGGCC GAGGGATCCA CGGTGGAGGA GTCCTCCAGT 
AGCTGCATCC GGCTGGAGTG GAACAAAGGC ATTGTGGGAC ATGTAGCTGC CACAGGACAG 
CCGCTCAACA TCAAGAACGC TTACGAGGAC TCCCGCTTTA ACGCCGAGGT GGACCTGATC 
ACGGGTTACA AAACCCACAG CATCCTGTGT CTGCCAATCA AGAACCACCG AGACGAGGTG 
GTCGGAGTAG CTCAGGCCAT CAATAAAAAA AGTGGAGATG ACGCTGCCTT CACTGACCAG 
GACGAAAAGG ATTTCTCCGC CTATTTGGCC TTTTCCGGCA TCGTCCTCCA CAACGCTCAG 
CTCTACGAGA CGTCCCAGCT GGAAAACAGA CGTAACCAGG TGCTGTTGGA CCTCGCCAGC 
CTCATCTTCG AGGAGCAGCA GTGCCTGAAG GTTTTACTCC GGAAGTTTGC GGGAACCATC 
TTGTCCTTCA TGCAGGCCCA GGCGTGCACG GTCTTCATCG CCGACGAGGA CTCTATGAAC 
TCCTTCTCCA GTGTCTTTCA CATGGAATAC GAGGAGTTGG GGGAAGTCCT GGATGTCCCA 
AAAAGGGACT GTGATGTCAG CCAGATCAAC TACATGTATG CTCAGTATGT CAAGAACACC 
ATGCAGACGC TCAACATTGC AGACGTGACC AAGGACCAGC GCTTCCCCTG GACAGGGGAG 
AATCCAGATC ACACCAATGA CCAGATCAAG AGTTTGCTCT GCACTCCCAT CAGGAACGGA 
AAGAAGGACA AAGTGATCGG TGTGTGTCAG CTGGTGAACA AGATGGACGA AGCCTCGGAC 
AGTGTGAAGG CTTTCAACAG GAACGATGAG CAATTCCTGG AGGCTTTTGC CATCTTCTGC 
GGCCTCGGCA TCCAGAACAC ACAGATGTAC GAGACGGTGG AGAGAGCCAT GGCCAAACAG 
GAAGTCACCC TGGAGGTCTT GTCATATCAC GCCACTGCCT CCGAGGAAGA ATCGCGGGAA 
CTCCAGGTCG CCACGGTGCC GTCCGCTCAG TCACTGCGGC TTCTGGACTT CAGCTTTAGC 
GACTTCGAGT TGTCCGACAC GGAAACCACG CTGGCCACCA TCCGCATGTT CGTCGACCTC 
AACCTGGTCC AGAACTTCCA GATGAAGTAC ATGAATTTAT GTCGATGGGT TCTGAGTGTG 
AAGAAGAACT ACAGGAAGAG CGTGGCGTAC CACAACTGGA GACACGCCTT CAACACGGCT 
CAGAGCATGT TTGCTCTCCT CAAGTCTGGA CGCTTGCAGA ACAACCTGAA CGACCTGGAG 
GTCTTAGCTC TGATGATTGC CACTCTGAGC CACGACCTGG ACCACAGAGG AGTCAACAAC 
TCCTACATAC AGAGGAGTGA CCACCCATTA GCCCAGCTCT ACTGCCACTC CACCATGGAG 
CATCACCACT TCGACCACTG CCTCATGATC CTCAACAGCC CTGGAAACCA GATCCTGAGC 
AGCCTGTCCC TGGACGAGTA CAAATCCACT CTGAAAATGA TCGAGAGGGC TATTCTGGCC 
ACGGACCTGG CCCTGTACAT GAAGCGGCGT GGGGAGTTCT TTGAGCTCAC CAAGAACAGT 
CAGTTTGTCT GGGAAGATGA GCAGCACAGA GACTTACTGA GGTCAATGCT GATGACTGCA 
TGTGACATCT CAGCCATCAC CAAACCCTGG CCCGTGCAAC AAAGGATTGC AGAACTGGTG 
GCGACCGAGT TCTTCGAGCA GGGAGATAAG GAAAGACGCG AGCTGAACAT TGAACCCAGC 
GACCTGATGA ATCGAGAGAA AAAAGACAAG ATCCCCAGCA TGCAGGTGTC CTTCATTGAC 
GCCATCTGTA CCCAGTTATA CGAGACATTG GCCGGCATGT CGGAGTACTG CTCCCCACTT 
TTGGAGGGCT GTCAGAAAAA TCGACAGCAG TGGAAGCATT TGGCCGAAGA GTGCGAGAAA 
GGATTGGTCA ACGGCTTGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696170.1
CDS	182..2845
Translation	MTTQLTHYLPETQKQPLQKMDDGCFMSNQHRPVQREMVNAWFAERVHSIHPSAPKENPPQHPRTQQSSADSAVSSASAVPPPTITLPDYCCPSPVTAPSPVPGTPTRKISASEFDRPLRPIVVKDAEGSLTFLSDADRETVPVRAAAMGEGGGVHVGGCDEAGSWCGRDRCARLLELVKEVSSHLDVTALCHKIFLHINELIAADRYSLFLVGEDSSNRKFLVSRLFDVAEGSTVEESSSSCIRLEWNKGIVGHVAATGQPLNIKNAYEDSRFNAEVDLITGYKTHSILCLPIKNHRDEVVGVAQAINKKSGDDAAFTDQDEKDFSAYLAFSGIVLHNAQLYETSQLENRRNQVLLDLASLIFEEQQCLKVLLRKFAGTILSFMQAQACTVFIADEDSMNSFSSVFHMEYEELGEVLDVPKRDCDVSQINYMYAQYVKNTMQTLNIADVTKDQRFPWTGENPDHTNDQIKSLLCTPIRNGKKDKVIGVCQLVNKMDEASDSVKAFNRNDEQFLEAFAIFCGLGIQNTQMYETVERAMAKQEVTLEVLSYHATASEEESRELQVATVPSAQSLRLLDFSFSDFELSDTETTLATIRMFVDLNLVQNFQMKYMNLCRWVLSVKKNYRKSVAYHNWRHAFNTAQSMFALLKSGRLQNNLNDLEVLALMIATLSHDLDHRGVNNSYIQRSDHPLAQLYCHSTMEHHHFDHCLMILNSPGNQILSSLSLDEYKSTLKMIERAILATDLALYMKRRGEFFELTKNSQFVWEDEQHRDLLRSMLMTACDISAITKPWPVQQRIAELVATEFFEQGDKERRELNIEPSDLMNREKKDKIPSMQVSFIDAICTQLYETLAGMSEYCSPLLEGCQKNRQQWKHLAEECEKGLVNGLV

Target ORF information:

RefSeq Version	XM_029840310.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840310.1

ORF Insert Sequence:

ATGACAACCC AACTGACTCA TTATCTCCCC GAGACACAAA AACAGCCACT ACAGAAGATG 
GACGACGGGT GTTTCATGTC CAACCAGCAC CGTCCGGTCC AGAGGGAGAT GGTCAACGCC 
TGGTTCGCTG AGCGAGTCCA CTCCATCCAT CCATCGGCTC CTAAAGAGAA TCCTCCTCAG 
CATCCCAGGA CCCAGCAGTC CTCTGCAGAC TCTGCTGTCT CCTCAGCCTC CGCCGTCCCC 
CCGCCCACCA TCACCCTGCC GGACTACTGC TGCCCCTCTC CAGTCACAGC CCCCAGCCCC 
GTCCCCGGCA CCCCGACTCG TAAAATCTCA GCTTCGGAAT TTGACCGCCC GCTCAGACCC 
ATTGTGGTCA AAGACGCCGA GGGTTCTTTG ACGTTCCTAA GCGACGCCGA CCGCGAGACT 
GTCCCCGTGC GGGCTGCAGC GATGGGCGAG GGCGGAGGAG TCCACGTCGG GGGTTGCGAC 
GAGGCAGGTA GTTGGTGCGG GCGCGATCGC TGCGCGAGGC TGCTGGAGCT GGTGAAGGAA 
GTGTCGAGTC ATCTGGATGT GACAGCACTC TGCCACAAGA TCTTCCTCCA CATCAACGAG 
CTGATCGCTG CAGACCGCTA CTCGCTCTTC CTGGTCGGCG AGGACAGCTC CAATCGAAAG 
TTTTTGGTCA GCCGCCTGTT TGATGTGGCC GAGGGATCCA CGGTGGAGGA GTCCTCCAGT 
AGCTGCATCC GGCTGGAGTG GAACAAAGGC ATTGTGGGAC ATGTAGCTGC CACAGGACAG 
CCGCTCAACA TCAAGAACGC TTACGAGGAC TCCCGCTTTA ACGCCGAGGT GGACCTGATC 
ACGGGTTACA AAACCCACAG CATCCTGTGT CTGCCAATCA AGAACCACCG AGACGAGGTG 
GTCGGAGTAG CTCAGGCCAT CAATAAAAAA AGTGGAGATG ACGCTGCCTT CACTGACCAG 
GACGAAAAGG ATTTCTCCGC CTATTTGGCC TTTTCCGGCA TCGTCCTCCA CAACGCTCAG 
CTCTACGAGA CGTCCCAGCT GGAAAACAGA CGTAACCAGG TGCTGTTGGA CCTCGCCAGC 
CTCATCTTCG AGGAGCAGCA GTGCCTGAAG GTTTTACTCC GGAAGTTTGC GGGAACCATC 
TTGTCCTTCA TGCAGGCCCA GGCGTGCACG GTCTTCATCG CCGACGAGGA CTCTATGAAC 
TCCTTCTCCA GTGTCTTTCA CATGGAATAC GAGGAGTTGG GGGAAGTCCT GGATGTCCCA 
AAAAGGGACT GTGATGTCAG CCAGATCAAC TACATGTATG CTCAGTATGT CAAGAACACC 
ATGCAGACGC TCAACATTGC AGACGTGACC AAGGACCAGC GCTTCCCCTG GACAGGGGAG 
AATCCAGATC ACACCAATGA CCAGATCAAG AGTTTGCTCT GCACTCCCAT CAGGAACGGA 
AAGAAGGACA AAGTGATCGG TGTGTGTCAG CTGGTGAACA AGATGGACGA AGCCTCGGAC 
AGTGTGAAGG CTTTCAACAG GAACGATGAG CAATTCCTGG AGGCTTTTGC CATCTTCTGC 
GGCCTCGGCA TCCAGAACAC ACAGATGTAC GAGACGGTGG AGAGAGCCAT GGCCAAACAG 
GAAGTCACCC TGGAGGTCTT GTCATATCAC GCCACTGCCT CCGAGGAAGA ATCGCGGGAA 
CTCCAGGTCG CCACGGTGCC GTCCGCTCAG TCACTGCGGC TTCTGGACTT CAGCTTTAGC 
GACTTCGAGT TGTCCGACAC GGAAACCACG CTGGCCACCA TCCGCATGTT CGTCGACCTC 
AACCTGGTCC AGAACTTCCA GATGAAGTAC ATGAATTTAT GTCGATGGGT TCTGAGTGTG 
AAGAAGAACT ACAGGAAGAG CGTGGCGTAC CACAACTGGA GACACGCCTT CAACACGGCT 
CAGAGCATGT TTGCTCTCCT CAAGTCTGGA CGCTTGCAGA ACAACCTGAA CGACCTGGAG 
GTCTTAGCTC TGATGATTGC CACTCTGAGC CACGACCTGG ACCACAGAGG AGTCAACAAC 
TCCTACATAC AGAGGAGTGA CCACCCATTA GCCCAGCTCT ACTGCCACTC CACCATGGAG 
CATCACCACT TCGACCACTG CCTCATGATC CTCAACAGCC CTGGAAACCA GATCCTGAGC 
AGCCTGTCCC TGGACGAGTA CAAATCCACT CTGAAAATGA TCGAGAGGGC TATTCTGGCC 
ACGGACCTGG CCCTGTACAT GAAGCGGCGT GGGGAGTTCT TTGAGCTCAC CAAGAACAGT 
CAGTTTGTCT GGGAAGATGA GCAGCACAGA GACTTACTGA GGTCAATGCT GATGACTGCA 
TGTGACATCT CAGCCATCAC CAAACCCTGG CCCGTGCAAC AAAGGATTGC AGAACTGGTG 
GCGACCGAGT TCTTCGAGCA GGGAGATAAG GAAAGACGCG AGCTGAACAT TGAACCCAGC 
GACCTGATGA ATCGAGAGAA AAAAGACAAG ATCCCCAGCA TGCAGGTGTC CTTCATTGAC 
GCCATCTGTA CCCAGTTATA CGAGACATTG GCCGGCATGT CGGAGTACTG CTCCCCACTT 
TTGGAGGGCT GTCAGAAAAA TCGACAGCAG TGGAAGCATT TGGCCGAAGA GTGCGAGAAA 
GGATTGGTCA ACGGCTTGGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde5a ( XM_029840311.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696171.1 Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo71198
Clone ID Related Accession (Same CDS sequence)	XM_029840311.1
Accession Version	XM_029840311.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2610bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAC TGAATTTGAA ATCGGCAGCT GGAGCCGACA TGGAGATGGT CAACGCCTGG 
TTCGCTGAGC GAGTCCACTC CATCCATCCA TCGGCTCCTA AAGAGAATCC TCCTCAGCAT 
CCCAGGACCC AGCAGTCCTC TGCAGACTCT GCTGTCTCCT CAGCCTCCGC CGTCCCCCCG 
CCCACCATCA CCCTGCCGGA CTACTGCTGC CCCTCTCCAG TCACAGCCCC CAGCCCCGTC 
CCCGGCACCC CGACTCGTAA AATCTCAGCT TCGGAATTTG ACCGCCCGCT CAGACCCATT 
GTGGTCAAAG ACGCCGAGGG TTCTTTGACG TTCCTAAGCG ACGCCGACCG CGAGACTGTC 
CCCGTGCGGG CTGCAGCGAT GGGCGAGGGC GGAGGAGTCC ACGTCGGGGG TTGCGACGAG 
GCAGGTAGTT GGTGCGGGCG CGATCGCTGC GCGAGGCTGC TGGAGCTGGT GAAGGAAGTG 
TCGAGTCATC TGGATGTGAC AGCACTCTGC CACAAGATCT TCCTCCACAT CAACGAGCTG 
ATCGCTGCAG ACCGCTACTC GCTCTTCCTG GTCGGCGAGG ACAGCTCCAA TCGAAAGTTT 
TTGGTCAGCC GCCTGTTTGA TGTGGCCGAG GGATCCACGG TGGAGGAGTC CTCCAGTAGC 
TGCATCCGGC TGGAGTGGAA CAAAGGCATT GTGGGACATG TAGCTGCCAC AGGACAGCCG 
CTCAACATCA AGAACGCTTA CGAGGACTCC CGCTTTAACG CCGAGGTGGA CCTGATCACG 
GGTTACAAAA CCCACAGCAT CCTGTGTCTG CCAATCAAGA ACCACCGAGA CGAGGTGGTC 
GGAGTAGCTC AGGCCATCAA TAAAAAAAGT GGAGATGACG CTGCCTTCAC TGACCAGGAC 
GAAAAGGATT TCTCCGCCTA TTTGGCCTTT TCCGGCATCG TCCTCCACAA CGCTCAGCTC 
TACGAGACGT CCCAGCTGGA AAACAGACGT AACCAGGTGC TGTTGGACCT CGCCAGCCTC 
ATCTTCGAGG AGCAGCAGTG CCTGAAGGTT TTACTCCGGA AGTTTGCGGG AACCATCTTG 
TCCTTCATGC AGGCCCAGGC GTGCACGGTC TTCATCGCCG ACGAGGACTC TATGAACTCC 
TTCTCCAGTG TCTTTCACAT GGAATACGAG GAGTTGGGGG AAGTCCTGGA TGTCCCAAAA 
AGGGACTGTG ATGTCAGCCA GATCAACTAC ATGTATGCTC AGTATGTCAA GAACACCATG 
CAGACGCTCA ACATTGCAGA CGTGACCAAG GACCAGCGCT TCCCCTGGAC AGGGGAGAAT 
CCAGATCACA CCAATGACCA GATCAAGAGT TTGCTCTGCA CTCCCATCAG GAACGGAAAG 
AAGGACAAAG TGATCGGTGT GTGTCAGCTG GTGAACAAGA TGGACGAAGC CTCGGACAGT 
GTGAAGGCTT TCAACAGGAA CGATGAGCAA TTCCTGGAGG CTTTTGCCAT CTTCTGCGGC 
CTCGGCATCC AGAACACACA GATGTACGAG ACGGTGGAGA GAGCCATGGC CAAACAGGAA 
GTCACCCTGG AGGTCTTGTC ATATCACGCC ACTGCCTCCG AGGAAGAATC GCGGGAACTC 
CAGGTAACCG TGGTCGCCAC GGTGCCGTCC GCTCAGTCAC TGCGGCTTCT GGACTTCAGC 
TTTAGCGACT TCGAGTTGTC CGACACGGAA ACCACGCTGG CCACCATCCG CATGTTCGTC 
GACCTCAACC TGGTCCAGAA CTTCCAGATG AAGTACATGA ATTTATGTCG ATGGGTTCTG 
AGTGTGAAGA AGAACTACAG GAAGAGCGTG GCGTACCACA ACTGGAGACA CGCCTTCAAC 
ACGGCTCAGA GCATGTTTGC TCTCCTCAAG TCTGGACGCT TGCAGAACAA CCTGAACGAC 
CTGGAGGTCT TAGCTCTGAT GATTGCCACT CTGAGCCACG ACCTGGACCA CAGAGGAGTC 
AACAACTCCT ACATACAGAG GAGTGACCAC CCATTAGCCC AGCTCTACTG CCACTCCACC 
ATGGAGCATC ACCACTTCGA CCACTGCCTC ATGATCCTCA ACAGCCCTGG AAACCAGATC 
CTGAGCAGCC TGTCCCTGGA CGAGTACAAA TCCACTCTGA AAATGATCGA GAGGGCTATT 
CTGGCCACGG ACCTGGCCCT GTACATGAAG CGGCGTGGGG AGTTCTTTGA GCTCACCAAG 
AACAGTCAGT TTGTCTGGGA AGATGAGCAG CACAGAGACT TACTGAGGTC AATGCTGATG 
ACTGCATGTG ACATCTCAGC CATCACCAAA CCCTGGCCCG TGCAACAAAG GATTGCAGAA 
CTGGTGGCGA CCGAGTTCTT CGAGCAGGGA GATAAGGAAA GACGCGAGCT GAACATTGAA 
CCCAGCGACC TGATGAATCG AGAGAAAAAA GACAAGATCC CCAGCATGCA GGTGTCCTTC 
ATTGACGCCA TCTGTACCCA GTTATACGAG ACATTGGCCG GCATGTCGGA GTACTGCTCC 
CCACTTTTGG AGGGCTGTCA GAAAAATCGA CAGCAGTGGA AGCATTTGGC CGAAGAGTGC 
GAGAAAGGAT TGGTCAACGG CTTGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696171.1
CDS	524..3133
Translation	MKSLNLKSAAGADMEMVNAWFAERVHSIHPSAPKENPPQHPRTQQSSADSAVSSASAVPPPTITLPDYCCPSPVTAPSPVPGTPTRKISASEFDRPLRPIVVKDAEGSLTFLSDADRETVPVRAAAMGEGGGVHVGGCDEAGSWCGRDRCARLLELVKEVSSHLDVTALCHKIFLHINELIAADRYSLFLVGEDSSNRKFLVSRLFDVAEGSTVEESSSSCIRLEWNKGIVGHVAATGQPLNIKNAYEDSRFNAEVDLITGYKTHSILCLPIKNHRDEVVGVAQAINKKSGDDAAFTDQDEKDFSAYLAFSGIVLHNAQLYETSQLENRRNQVLLDLASLIFEEQQCLKVLLRKFAGTILSFMQAQACTVFIADEDSMNSFSSVFHMEYEELGEVLDVPKRDCDVSQINYMYAQYVKNTMQTLNIADVTKDQRFPWTGENPDHTNDQIKSLLCTPIRNGKKDKVIGVCQLVNKMDEASDSVKAFNRNDEQFLEAFAIFCGLGIQNTQMYETVERAMAKQEVTLEVLSYHATASEEESRELQVTVVATVPSAQSLRLLDFSFSDFELSDTETTLATIRMFVDLNLVQNFQMKYMNLCRWVLSVKKNYRKSVAYHNWRHAFNTAQSMFALLKSGRLQNNLNDLEVLALMIATLSHDLDHRGVNNSYIQRSDHPLAQLYCHSTMEHHHFDHCLMILNSPGNQILSSLSLDEYKSTLKMIERAILATDLALYMKRRGEFFELTKNSQFVWEDEQHRDLLRSMLMTACDISAITKPWPVQQRIAELVATEFFEQGDKERRELNIEPSDLMNREKKDKIPSMQVSFIDAICTQLYETLAGMSEYCSPLLEGCQKNRQQWKHLAEECEKGLVNGLV

Target ORF information:

RefSeq Version	XM_029840311.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840311.1

ORF Insert Sequence:

ATGAAGTCAC TGAATTTGAA ATCGGCAGCT GGAGCCGACA TGGAGATGGT CAACGCCTGG 
TTCGCTGAGC GAGTCCACTC CATCCATCCA TCGGCTCCTA AAGAGAATCC TCCTCAGCAT 
CCCAGGACCC AGCAGTCCTC TGCAGACTCT GCTGTCTCCT CAGCCTCCGC CGTCCCCCCG 
CCCACCATCA CCCTGCCGGA CTACTGCTGC CCCTCTCCAG TCACAGCCCC CAGCCCCGTC 
CCCGGCACCC CGACTCGTAA AATCTCAGCT TCGGAATTTG ACCGCCCGCT CAGACCCATT 
GTGGTCAAAG ACGCCGAGGG TTCTTTGACG TTCCTAAGCG ACGCCGACCG CGAGACTGTC 
CCCGTGCGGG CTGCAGCGAT GGGCGAGGGC GGAGGAGTCC ACGTCGGGGG TTGCGACGAG 
GCAGGTAGTT GGTGCGGGCG CGATCGCTGC GCGAGGCTGC TGGAGCTGGT GAAGGAAGTG 
TCGAGTCATC TGGATGTGAC AGCACTCTGC CACAAGATCT TCCTCCACAT CAACGAGCTG 
ATCGCTGCAG ACCGCTACTC GCTCTTCCTG GTCGGCGAGG ACAGCTCCAA TCGAAAGTTT 
TTGGTCAGCC GCCTGTTTGA TGTGGCCGAG GGATCCACGG TGGAGGAGTC CTCCAGTAGC 
TGCATCCGGC TGGAGTGGAA CAAAGGCATT GTGGGACATG TAGCTGCCAC AGGACAGCCG 
CTCAACATCA AGAACGCTTA CGAGGACTCC CGCTTTAACG CCGAGGTGGA CCTGATCACG 
GGTTACAAAA CCCACAGCAT CCTGTGTCTG CCAATCAAGA ACCACCGAGA CGAGGTGGTC 
GGAGTAGCTC AGGCCATCAA TAAAAAAAGT GGAGATGACG CTGCCTTCAC TGACCAGGAC 
GAAAAGGATT TCTCCGCCTA TTTGGCCTTT TCCGGCATCG TCCTCCACAA CGCTCAGCTC 
TACGAGACGT CCCAGCTGGA AAACAGACGT AACCAGGTGC TGTTGGACCT CGCCAGCCTC 
ATCTTCGAGG AGCAGCAGTG CCTGAAGGTT TTACTCCGGA AGTTTGCGGG AACCATCTTG 
TCCTTCATGC AGGCCCAGGC GTGCACGGTC TTCATCGCCG ACGAGGACTC TATGAACTCC 
TTCTCCAGTG TCTTTCACAT GGAATACGAG GAGTTGGGGG AAGTCCTGGA TGTCCCAAAA 
AGGGACTGTG ATGTCAGCCA GATCAACTAC ATGTATGCTC AGTATGTCAA GAACACCATG 
CAGACGCTCA ACATTGCAGA CGTGACCAAG GACCAGCGCT TCCCCTGGAC AGGGGAGAAT 
CCAGATCACA CCAATGACCA GATCAAGAGT TTGCTCTGCA CTCCCATCAG GAACGGAAAG 
AAGGACAAAG TGATCGGTGT GTGTCAGCTG GTGAACAAGA TGGACGAAGC CTCGGACAGT 
GTGAAGGCTT TCAACAGGAA CGATGAGCAA TTCCTGGAGG CTTTTGCCAT CTTCTGCGGC 
CTCGGCATCC AGAACACACA GATGTACGAG ACGGTGGAGA GAGCCATGGC CAAACAGGAA 
GTCACCCTGG AGGTCTTGTC ATATCACGCC ACTGCCTCCG AGGAAGAATC GCGGGAACTC 
CAGGTAACCG TGGTCGCCAC GGTGCCGTCC GCTCAGTCAC TGCGGCTTCT GGACTTCAGC 
TTTAGCGACT TCGAGTTGTC CGACACGGAA ACCACGCTGG CCACCATCCG CATGTTCGTC 
GACCTCAACC TGGTCCAGAA CTTCCAGATG AAGTACATGA ATTTATGTCG ATGGGTTCTG 
AGTGTGAAGA AGAACTACAG GAAGAGCGTG GCGTACCACA ACTGGAGACA CGCCTTCAAC 
ACGGCTCAGA GCATGTTTGC TCTCCTCAAG TCTGGACGCT TGCAGAACAA CCTGAACGAC 
CTGGAGGTCT TAGCTCTGAT GATTGCCACT CTGAGCCACG ACCTGGACCA CAGAGGAGTC 
AACAACTCCT ACATACAGAG GAGTGACCAC CCATTAGCCC AGCTCTACTG CCACTCCACC 
ATGGAGCATC ACCACTTCGA CCACTGCCTC ATGATCCTCA ACAGCCCTGG AAACCAGATC 
CTGAGCAGCC TGTCCCTGGA CGAGTACAAA TCCACTCTGA AAATGATCGA GAGGGCTATT 
CTGGCCACGG ACCTGGCCCT GTACATGAAG CGGCGTGGGG AGTTCTTTGA GCTCACCAAG 
AACAGTCAGT TTGTCTGGGA AGATGAGCAG CACAGAGACT TACTGAGGTC AATGCTGATG 
ACTGCATGTG ACATCTCAGC CATCACCAAA CCCTGGCCCG TGCAACAAAG GATTGCAGAA 
CTGGTGGCGA CCGAGTTCTT CGAGCAGGGA GATAAGGAAA GACGCGAGCT GAACATTGAA 
CCCAGCGACC TGATGAATCG AGAGAAAAAA GACAAGATCC CCAGCATGCA GGTGTCCTTC 
ATTGACGCCA TCTGTACCCA GTTATACGAG ACATTGGCCG GCATGTCGGA GTACTGCTCC 
CCACTTTTGG AGGGCTGTCA GAAAAATCGA CAGCAGTGGA AGCATTTGGC CGAAGAGTGC 
GAGAAAGGAT TGGTCAACGG CTTGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde5a ( XM_029840312.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029696172.1 Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X5, mRNA.

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CloneID	OTo71199
Clone ID Related Accession (Same CDS sequence)	XM_029840312.1
Accession Version	XM_029840312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	cGMP-specific 3',5'-cyclic phosphodiesterase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042292.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAACG CCTGGTTCGC TGAGCGAGTC CACTCCATCC ATCCATCGGC TCCTAAAGAG 
AATCCTCCTC AGCATCCCAG GACCCAGCAG TCCTCTGCAG ACTCTGCTGT CTCCTCAGCC 
TCCGCCGTCC CCCCGCCCAC CATCACCCTG CCGGACTACT GCTGCCCCTC TCCAGTCACA 
GCCCCCAGCC CCGTCCCCGG CACCCCGACT CGTAAAATCT CAGCTTCGGA ATTTGACCGC 
CCGCTCAGAC CCATTGTGGT CAAAGACGCC GAGGGTTCTT TGACGTTCCT AAGCGACGCC 
GACCGCGAGA CTGTCCCCGT GCGGGCTGCA GCGATGGGCG AGGGCGGAGG AGTCCACGTC 
GGGGGTTGCG ACGAGGCAGG TAGTTGGTGC GGGCGCGATC GCTGCGCGAG GCTGCTGGAG 
CTGGTGAAGG AAGTGTCGAG TCATCTGGAT GTGACAGCAC TCTGCCACAA GATCTTCCTC 
CACATCAACG AGCTGATCGC TGCAGACCGC TACTCGCTCT TCCTGGTCGG CGAGGACAGC 
TCCAATCGAA AGTTTTTGGT CAGCCGCCTG TTTGATGTGG CCGAGGGATC CACGGTGGAG 
GAGTCCTCCA GTAGCTGCAT CCGGCTGGAG TGGAACAAAG GCATTGTGGG ACATGTAGCT 
GCCACAGGAC AGCCGCTCAA CATCAAGAAC GCTTACGAGG ACTCCCGCTT TAACGCCGAG 
GTGGACCTGA TCACGGGTTA CAAAACCCAC AGCATCCTGT GTCTGCCAAT CAAGAACCAC 
CGAGACGAGG TGGTCGGAGT AGCTCAGGCC ATCAATAAAA AAAGTGGAGA TGACGCTGCC 
TTCACTGACC AGGACGAAAA GGATTTCTCC GCCTATTTGG CCTTTTCCGG CATCGTCCTC 
CACAACGCTC AGCTCTACGA GACGTCCCAG CTGGAAAACA GACGTAACCA GGTGCTGTTG 
GACCTCGCCA GCCTCATCTT CGAGGAGCAG CAGTGCCTGA AGGTTTTACT CCGGAAGTTT 
GCGGGAACCA TCTTGTCCTT CATGCAGGCC CAGGCGTGCA CGGTCTTCAT CGCCGACGAG 
GACTCTATGA ACTCCTTCTC CAGTGTCTTT CACATGGAAT ACGAGGAGTT GGGGGAAGTC 
CTGGATGTCC CAAAAAGGGA CTGTGATGTC AGCCAGATCA ACTACATGTA TGCTCAGTAT 
GTCAAGAACA CCATGCAGAC GCTCAACATT GCAGACGTGA CCAAGGACCA GCGCTTCCCC 
TGGACAGGGG AGAATCCAGA TCACACCAAT GACCAGATCA AGAGTTTGCT CTGCACTCCC 
ATCAGGAACG GAAAGAAGGA CAAAGTGATC GGTGTGTGTC AGCTGGTGAA CAAGATGGAC 
GAAGCCTCGG ACAGTGTGAA GGCTTTCAAC AGGAACGATG AGCAATTCCT GGAGGCTTTT 
GCCATCTTCT GCGGCCTCGG CATCCAGAAC ACACAGATGT ACGAGACGGT GGAGAGAGCC 
ATGGCCAAAC AGGAAGTCAC CCTGGAGGTC TTGTCATATC ACGCCACTGC CTCCGAGGAA 
GAATCGCGGG AACTCCAGGT AACCGTGGTC GCCACGGTGC CGTCCGCTCA GTCACTGCGG 
CTTCTGGACT TCAGCTTTAG CGACTTCGAG TTGTCCGACA CGGAAACCAC GCTGGCCACC 
ATCCGCATGT TCGTCGACCT CAACCTGGTC CAGAACTTCC AGATGAAGTA CATGAATTTA 
TGTCGATGGG TTCTGAGTGT GAAGAAGAAC TACAGGAAGA GCGTGGCGTA CCACAACTGG 
AGACACGCCT TCAACACGGC TCAGAGCATG TTTGCTCTCC TCAAGTCTGG ACGCTTGCAG 
AACAACCTGA ACGACCTGGA GGTCTTAGCT CTGATGATTG CCACTCTGAG CCACGACCTG 
GACCACAGAG GAGTCAACAA CTCCTACATA CAGAGGAGTG ACCACCCATT AGCCCAGCTC 
TACTGCCACT CCACCATGGA GCATCACCAC TTCGACCACT GCCTCATGAT CCTCAACAGC 
CCTGGAAACC AGATCCTGAG CAGCCTGTCC CTGGACGAGT ACAAATCCAC TCTGAAAATG 
ATCGAGAGGG CTATTCTGGC CACGGACCTG GCCCTGTACA TGAAGCGGCG TGGGGAGTTC 
TTTGAGCTCA CCAAGAACAG TCAGTTTGTC TGGGAAGATG AGCAGCACAG AGACTTACTG 
AGGTCAATGC TGATGACTGC ATGTGACATC TCAGCCATCA CCAAACCCTG GCCCGTGCAA 
CAAAGGATTG CAGAACTGGT GGCGACCGAG TTCTTCGAGC AGGGAGATAA GGAAAGACGC 
GAGCTGAACA TTGAACCCAG CGACCTGATG AATCGAGAGA AAAAAGACAA GATCCCCAGC 
ATGCAGGTGT CCTTCATTGA CGCCATCTGT ACCCAGTTAT ACGAGACATT GGCCGGCATG 
TCGGAGTACT GCTCCCCACT TTTGGAGGGC TGTCAGAAAA ATCGACAGCA GTGGAAGCAT 
TTGGCCGAAG AGTGCGAGAA AGGATTGGTC AACGGCTTGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029696172.1
CDS	189..2753
Translation	MVNAWFAERVHSIHPSAPKENPPQHPRTQQSSADSAVSSASAVPPPTITLPDYCCPSPVTAPSPVPGTPTRKISASEFDRPLRPIVVKDAEGSLTFLSDADRETVPVRAAAMGEGGGVHVGGCDEAGSWCGRDRCARLLELVKEVSSHLDVTALCHKIFLHINELIAADRYSLFLVGEDSSNRKFLVSRLFDVAEGSTVEESSSSCIRLEWNKGIVGHVAATGQPLNIKNAYEDSRFNAEVDLITGYKTHSILCLPIKNHRDEVVGVAQAINKKSGDDAAFTDQDEKDFSAYLAFSGIVLHNAQLYETSQLENRRNQVLLDLASLIFEEQQCLKVLLRKFAGTILSFMQAQACTVFIADEDSMNSFSSVFHMEYEELGEVLDVPKRDCDVSQINYMYAQYVKNTMQTLNIADVTKDQRFPWTGENPDHTNDQIKSLLCTPIRNGKKDKVIGVCQLVNKMDEASDSVKAFNRNDEQFLEAFAIFCGLGIQNTQMYETVERAMAKQEVTLEVLSYHATASEEESRELQVTVVATVPSAQSLRLLDFSFSDFELSDTETTLATIRMFVDLNLVQNFQMKYMNLCRWVLSVKKNYRKSVAYHNWRHAFNTAQSMFALLKSGRLQNNLNDLEVLALMIATLSHDLDHRGVNNSYIQRSDHPLAQLYCHSTMEHHHFDHCLMILNSPGNQILSSLSLDEYKSTLKMIERAILATDLALYMKRRGEFFELTKNSQFVWEDEQHRDLLRSMLMTACDISAITKPWPVQQRIAELVATEFFEQGDKERRELNIEPSDLMNREKKDKIPSMQVSFIDAICTQLYETLAGMSEYCSPLLEGCQKNRQQWKHLAEECEKGLVNGLV

Target ORF information:

RefSeq Version	XM_029840312.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes phosphodiesterase 5A (pde5a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029840312.1

ORF Insert Sequence:

ATGGTCAACG CCTGGTTCGC TGAGCGAGTC CACTCCATCC ATCCATCGGC TCCTAAAGAG 
AATCCTCCTC AGCATCCCAG GACCCAGCAG TCCTCTGCAG ACTCTGCTGT CTCCTCAGCC 
TCCGCCGTCC CCCCGCCCAC CATCACCCTG CCGGACTACT GCTGCCCCTC TCCAGTCACA 
GCCCCCAGCC CCGTCCCCGG CACCCCGACT CGTAAAATCT CAGCTTCGGA ATTTGACCGC 
CCGCTCAGAC CCATTGTGGT CAAAGACGCC GAGGGTTCTT TGACGTTCCT AAGCGACGCC 
GACCGCGAGA CTGTCCCCGT GCGGGCTGCA GCGATGGGCG AGGGCGGAGG AGTCCACGTC 
GGGGGTTGCG ACGAGGCAGG TAGTTGGTGC GGGCGCGATC GCTGCGCGAG GCTGCTGGAG 
CTGGTGAAGG AAGTGTCGAG TCATCTGGAT GTGACAGCAC TCTGCCACAA GATCTTCCTC 
CACATCAACG AGCTGATCGC TGCAGACCGC TACTCGCTCT TCCTGGTCGG CGAGGACAGC 
TCCAATCGAA AGTTTTTGGT CAGCCGCCTG TTTGATGTGG CCGAGGGATC CACGGTGGAG 
GAGTCCTCCA GTAGCTGCAT CCGGCTGGAG TGGAACAAAG GCATTGTGGG ACATGTAGCT 
GCCACAGGAC AGCCGCTCAA CATCAAGAAC GCTTACGAGG ACTCCCGCTT TAACGCCGAG 
GTGGACCTGA TCACGGGTTA CAAAACCCAC AGCATCCTGT GTCTGCCAAT CAAGAACCAC 
CGAGACGAGG TGGTCGGAGT AGCTCAGGCC ATCAATAAAA AAAGTGGAGA TGACGCTGCC 
TTCACTGACC AGGACGAAAA GGATTTCTCC GCCTATTTGG CCTTTTCCGG CATCGTCCTC 
CACAACGCTC AGCTCTACGA GACGTCCCAG CTGGAAAACA GACGTAACCA GGTGCTGTTG 
GACCTCGCCA GCCTCATCTT CGAGGAGCAG CAGTGCCTGA AGGTTTTACT CCGGAAGTTT 
GCGGGAACCA TCTTGTCCTT CATGCAGGCC CAGGCGTGCA CGGTCTTCAT CGCCGACGAG 
GACTCTATGA ACTCCTTCTC CAGTGTCTTT CACATGGAAT ACGAGGAGTT GGGGGAAGTC 
CTGGATGTCC CAAAAAGGGA CTGTGATGTC AGCCAGATCA ACTACATGTA TGCTCAGTAT 
GTCAAGAACA CCATGCAGAC GCTCAACATT GCAGACGTGA CCAAGGACCA GCGCTTCCCC 
TGGACAGGGG AGAATCCAGA TCACACCAAT GACCAGATCA AGAGTTTGCT CTGCACTCCC 
ATCAGGAACG GAAAGAAGGA CAAAGTGATC GGTGTGTGTC AGCTGGTGAA CAAGATGGAC 
GAAGCCTCGG ACAGTGTGAA GGCTTTCAAC AGGAACGATG AGCAATTCCT GGAGGCTTTT 
GCCATCTTCT GCGGCCTCGG CATCCAGAAC ACACAGATGT ACGAGACGGT GGAGAGAGCC 
ATGGCCAAAC AGGAAGTCAC CCTGGAGGTC TTGTCATATC ACGCCACTGC CTCCGAGGAA 
GAATCGCGGG AACTCCAGGT AACCGTGGTC GCCACGGTGC CGTCCGCTCA GTCACTGCGG 
CTTCTGGACT TCAGCTTTAG CGACTTCGAG TTGTCCGACA CGGAAACCAC GCTGGCCACC 
ATCCGCATGT TCGTCGACCT CAACCTGGTC CAGAACTTCC AGATGAAGTA CATGAATTTA 
TGTCGATGGG TTCTGAGTGT GAAGAAGAAC TACAGGAAGA GCGTGGCGTA CCACAACTGG 
AGACACGCCT TCAACACGGC TCAGAGCATG TTTGCTCTCC TCAAGTCTGG ACGCTTGCAG 
AACAACCTGA ACGACCTGGA GGTCTTAGCT CTGATGATTG CCACTCTGAG CCACGACCTG 
GACCACAGAG GAGTCAACAA CTCCTACATA CAGAGGAGTG ACCACCCATT AGCCCAGCTC 
TACTGCCACT CCACCATGGA GCATCACCAC TTCGACCACT GCCTCATGAT CCTCAACAGC 
CCTGGAAACC AGATCCTGAG CAGCCTGTCC CTGGACGAGT ACAAATCCAC TCTGAAAATG 
ATCGAGAGGG CTATTCTGGC CACGGACCTG GCCCTGTACA TGAAGCGGCG TGGGGAGTTC 
TTTGAGCTCA CCAAGAACAG TCAGTTTGTC TGGGAAGATG AGCAGCACAG AGACTTACTG 
AGGTCAATGC TGATGACTGC ATGTGACATC TCAGCCATCA CCAAACCCTG GCCCGTGCAA 
CAAAGGATTG CAGAACTGGT GGCGACCGAG TTCTTCGAGC AGGGAGATAA GGAAAGACGC 
GAGCTGAACA TTGAACCCAG CGACCTGATG AATCGAGAGA AAAAAGACAA GATCCCCAGC 
ATGCAGGTGT CCTTCATTGA CGCCATCTGT ACCCAGTTAT ACGAGACATT GGCCGGCATG 
TCGGAGTACT GCTCCCCACT TTTGGAGGGC TGTCAGAAAA ATCGACAGCA GTGGAAGCAT 
TTGGCCGAAG AGTGCGAGAA AGGATTGGTC AACGGCTTGG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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pde5a cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム