man2a2 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene
Clones
gRNAs

Summary

Gene Symbol	man2a2
Entrez Gene ID	101061792
Full Name	mannosidase alpha class 2A member 2
Gene Type	protein-coding
Organism	Takifugu rubripes(torafugu)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011607291.1	XP_011605593.1	alpha-mannosidase 2x
XM_011607294.1	XP_011605596.1	alpha-mannosidase 2x
XM_011607293.2	XP_011605595.1	alpha-mannosidase 2x isoform X1
XM_029842068.1	XP_029697928.1	alpha-mannosidase 2x isoform X2
XM_011607292.1	XP_011605594.1	alpha-mannosidase 2x
XM_011607293.1	XP_011605595.1	alpha-mannosidase 2x
XM_011607291.2	XP_011605593.1	alpha-mannosidase 2x isoform X1
XM_011607292.2	XP_011605594.1	alpha-mannosidase 2x isoform X1

Pathways from BioSystems
Gene Ontology

The following man2a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the man2a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OTo57809	XM_011607293.2 Latest version!	Takifugu rubripes mannosidase alpha class 2A member 2 (man2a2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo57809	XM_011607291.2 Latest version!	Takifugu rubripes mannosidase alpha class 2A member 2 (man2a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo57809	XM_011607292.2 Latest version!	Takifugu rubripes mannosidase alpha class 2A member 2 (man2a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo57810	XM_029842068.1 Latest version!	Takifugu rubripes mannosidase alpha class 2A member 2 (man2a2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OTo19125	XM_011607293.1 Latest version!	Takifugu rubripes mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OTo19125	XM_011607292.1 Latest version!	Takifugu rubripes mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OTo19125	XM_011607291.1 Latest version!	Takifugu rubripes mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OTo19125	XM_011607294.1 Latest version!	Takifugu rubripes mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

man2a2 ( XM_011607293.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605595.1 Takifugu rubripes mannosidase alpha class 2A member 2 (man2a2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OTo57809
Clone ID Related Accession (Same CDS sequence)	XM_011607293.2 , XM_011607291.2 , XM_011607292.2
Accession Version	XM_011607293.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042293.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011607293.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTAGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACAC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTTCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605595.1
CDS	139..3597
Translation	MKLRKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQSGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVMELTDTGAVSPSGHLPFRSANGSWVLPFDGRPTFLALKPQDCQFTAGSHGQADVQMLDVYSLLKFDNPDGGVWKQGFDITYEPDEWDREPLQVFVVPHSHNDPGWIKTFDKYFMEQTQHILNNMVVKLLEDPRRKFIWSEISFFAKWWETADTYKQEAARKLILGGQLEIVTGGWVMTDEANVHYFAMLDQLIEGHQWLERNLGVTPRSAWAVDPFGHSATMPYMLKRSNLTSMLIQRVHYSIKKHFASTRSLEFMWRQGWDTGSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPKTVVEANVAERANLLLDQYRKKSKLYRSKVLLVPLGDDFRYDKALEWDQQYTNYQKLFDYMNSHPELHVQAQFGTLSDYFNAVYKAHGVAQGSRPADYPVLSGDFFAYADREDHYWTGYFTSRPFYKSMDRVIESHLRGAEILYSLAIANARHAGMEGRYPISDYGLLVDARRSVGLFQHHDAITGTAKENVVIDYGNKLLRSLIGLKRVIINAAHFLVMKNKEFYRFYQTEPFLETDDRRATQDSLPQRTLIELDPAGPRYLVVFNPIEQERLCTVTVLVNTVKVRVLTEDGQTLPVQLSAQWSSASQMSAEVFEATFMVRLPPLGLAVFHLYESPDSPMTLRSETLLRLSSRGVAARAVDPLPVRSQQSDPQTFYISSQSLTLGFSGTTGLLESIKRKDDSQEVKVQMQFMMYGTRPSKDKSGAYLFLPDGKAKPYNQKEPPVVRVVEGPLFSEVVAYYQHFQQSIRIYNVPGVDGFSVDVATAVDIRDQNNKELSMRLVTNIQSGDLFYTDLNGFQIQPRRHHLKLPLQANFYPMPSQAYIQDSNYRLTMHTAQALGVSSLESGQLEVILDRRLMQDDNRGLGQGLKDNKKTINRFRLLLERRSMSNKMMDSTSSSFPSILSHMTSSFLNLDVLALPVIPKRRGVPPLQTFAPLKSILPCDFRLVNLRSIQTQQDPQSPSPYTALILHRLPLDCGLETPNLGFNCTTTQGQLTVSRLFKNLDLQLLQPMSLTLMYSSPPLANESTVTLDPMEITTFKLKLR

Target ORF information:

RefSeq Version	XM_011607293.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes mannosidase alpha class 2A member 2 (man2a2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607293.2

ORF Insert Sequence:

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTAGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACAC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTTCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_011607291.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605593.1 Takifugu rubripes mannosidase alpha class 2A member 2 (man2a2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OTo57809
Clone ID Related Accession (Same CDS sequence)	XM_011607293.2 , XM_011607291.2 , XM_011607292.2
Accession Version	XM_011607291.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042293.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011607291.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTAGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACAC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTTCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605593.1
CDS	838..4296
Translation	MKLRKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQSGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVMELTDTGAVSPSGHLPFRSANGSWVLPFDGRPTFLALKPQDCQFTAGSHGQADVQMLDVYSLLKFDNPDGGVWKQGFDITYEPDEWDREPLQVFVVPHSHNDPGWIKTFDKYFMEQTQHILNNMVVKLLEDPRRKFIWSEISFFAKWWETADTYKQEAARKLILGGQLEIVTGGWVMTDEANVHYFAMLDQLIEGHQWLERNLGVTPRSAWAVDPFGHSATMPYMLKRSNLTSMLIQRVHYSIKKHFASTRSLEFMWRQGWDTGSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPKTVVEANVAERANLLLDQYRKKSKLYRSKVLLVPLGDDFRYDKALEWDQQYTNYQKLFDYMNSHPELHVQAQFGTLSDYFNAVYKAHGVAQGSRPADYPVLSGDFFAYADREDHYWTGYFTSRPFYKSMDRVIESHLRGAEILYSLAIANARHAGMEGRYPISDYGLLVDARRSVGLFQHHDAITGTAKENVVIDYGNKLLRSLIGLKRVIINAAHFLVMKNKEFYRFYQTEPFLETDDRRATQDSLPQRTLIELDPAGPRYLVVFNPIEQERLCTVTVLVNTVKVRVLTEDGQTLPVQLSAQWSSASQMSAEVFEATFMVRLPPLGLAVFHLYESPDSPMTLRSETLLRLSSRGVAARAVDPLPVRSQQSDPQTFYISSQSLTLGFSGTTGLLESIKRKDDSQEVKVQMQFMMYGTRPSKDKSGAYLFLPDGKAKPYNQKEPPVVRVVEGPLFSEVVAYYQHFQQSIRIYNVPGVDGFSVDVATAVDIRDQNNKELSMRLVTNIQSGDLFYTDLNGFQIQPRRHHLKLPLQANFYPMPSQAYIQDSNYRLTMHTAQALGVSSLESGQLEVILDRRLMQDDNRGLGQGLKDNKKTINRFRLLLERRSMSNKMMDSTSSSFPSILSHMTSSFLNLDVLALPVIPKRRGVPPLQTFAPLKSILPCDFRLVNLRSIQTQQDPQSPSPYTALILHRLPLDCGLETPNLGFNCTTTQGQLTVSRLFKNLDLQLLQPMSLTLMYSSPPLANESTVTLDPMEITTFKLKLR

Target ORF information:

RefSeq Version	XM_011607291.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes mannosidase alpha class 2A member 2 (man2a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607291.2

ORF Insert Sequence:

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTAGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACAC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTTCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_011607292.2 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605594.1 Takifugu rubripes mannosidase alpha class 2A member 2 (man2a2), transcript variant X2, mRNA.

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CloneID	OTo57809
Clone ID Related Accession (Same CDS sequence)	XM_011607293.2 , XM_011607291.2 , XM_011607292.2
Accession Version	XM_011607292.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	alpha-mannosidase 2x isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042293.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 5, 2019 this sequence version replaced XM_011607292.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTAGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACAC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTTCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605594.1
CDS	714..4172
Translation	MKLRKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQSGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVMELTDTGAVSPSGHLPFRSANGSWVLPFDGRPTFLALKPQDCQFTAGSHGQADVQMLDVYSLLKFDNPDGGVWKQGFDITYEPDEWDREPLQVFVVPHSHNDPGWIKTFDKYFMEQTQHILNNMVVKLLEDPRRKFIWSEISFFAKWWETADTYKQEAARKLILGGQLEIVTGGWVMTDEANVHYFAMLDQLIEGHQWLERNLGVTPRSAWAVDPFGHSATMPYMLKRSNLTSMLIQRVHYSIKKHFASTRSLEFMWRQGWDTGSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPKTVVEANVAERANLLLDQYRKKSKLYRSKVLLVPLGDDFRYDKALEWDQQYTNYQKLFDYMNSHPELHVQAQFGTLSDYFNAVYKAHGVAQGSRPADYPVLSGDFFAYADREDHYWTGYFTSRPFYKSMDRVIESHLRGAEILYSLAIANARHAGMEGRYPISDYGLLVDARRSVGLFQHHDAITGTAKENVVIDYGNKLLRSLIGLKRVIINAAHFLVMKNKEFYRFYQTEPFLETDDRRATQDSLPQRTLIELDPAGPRYLVVFNPIEQERLCTVTVLVNTVKVRVLTEDGQTLPVQLSAQWSSASQMSAEVFEATFMVRLPPLGLAVFHLYESPDSPMTLRSETLLRLSSRGVAARAVDPLPVRSQQSDPQTFYISSQSLTLGFSGTTGLLESIKRKDDSQEVKVQMQFMMYGTRPSKDKSGAYLFLPDGKAKPYNQKEPPVVRVVEGPLFSEVVAYYQHFQQSIRIYNVPGVDGFSVDVATAVDIRDQNNKELSMRLVTNIQSGDLFYTDLNGFQIQPRRHHLKLPLQANFYPMPSQAYIQDSNYRLTMHTAQALGVSSLESGQLEVILDRRLMQDDNRGLGQGLKDNKKTINRFRLLLERRSMSNKMMDSTSSSFPSILSHMTSSFLNLDVLALPVIPKRRGVPPLQTFAPLKSILPCDFRLVNLRSIQTQQDPQSPSPYTALILHRLPLDCGLETPNLGFNCTTTQGQLTVSRLFKNLDLQLLQPMSLTLMYSSPPLANESTVTLDPMEITTFKLKLR

Target ORF information:

RefSeq Version	XM_011607292.2
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes mannosidase alpha class 2A member 2 (man2a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607292.2

ORF Insert Sequence:

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTAGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACAC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTTCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_029842068.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_029697928.1 Takifugu rubripes mannosidase alpha class 2A member 2 (man2a2), transcript variant X4, mRNA.

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CloneID	OTo57810
Clone ID Related Accession (Same CDS sequence)	XM_029842068.1
Accession Version	XM_029842068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Takifugu rubripes(torafugu)
Product	alpha-mannosidase 2x isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_042293.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Takifugu rubripes Annotation Release 103 Annotation Version :: 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTAGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACAC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTTCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
GACAGCACAT CGTCCAGCTT CCCCTCCATT CTCAGTCACA TGACCAGCTC CTTCCTGAAC 
CTAGACGTCT TGGCGCTGCC TGTAATTCCC AAAAGACGCG GCGTCCCTCC TCTGCAGACC 
TTCGCCCCTC TCAAGTCCAT CCTGCCCTGT GACTTTCGCT TGGTCAACCT GCGCAGCATC 
CAGACCCAGC AGGACCCCCA ATCTCCATCT CCGTACACAG CCTTGATTCT TCACCGTCTG 
CCGTTGGATT GTGGCCTGGA GACTCCGAAC CTGGGCTTTA ACTGCACTAC CACACAGGGG 
CAGCTAACTG TATCGAGACT GTTCAAGAAT CTGGACCTGC AGCTCCTCCA GCCCATGTCC 
CTGACTCTGA TGTACTCCAG CCCACCTCTG GCCAATGAAT CCACCGTCAC CCTGGATCCC 
ATGGAGATCA CCACCTTCAA GCTCAAACTA CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_029697928.1
CDS	838..4293
Translation	MKLRKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQSGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVMELTDTGAVSPSGHLPFRSANGSWVLPFDGRPTFLALKPQDCQFTAGSHGQADVQMLDVYSLLKFDNPDGGVWKQGFDITYEPDEWDREPLQVFVVPHSHNDPGWIKTFDKYFMEQTQHILNNMVVKLLEDPRRKFIWSEISFFAKWWETADTYKQEAARKLILGGQLEIVTGGWVMTDEANVHYFAMLDQLIEGHQWLERNLGVTPRSAWAVDPFGHSATMPYMLKRSNLTSMLIQRVHYSIKKHFASTRSLEFMWRQGWDTGSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPKTVVEANVAERANLLLDQYRKKSKLYRSKVLLVPLGDDFRYDKALEWDQQYTNYQKLFDYMNSHPELHVQAQFGTLSDYFNAVYKAHGVAQGSRPADYPVLSGDFFAYADREDHYWTGYFTSRPFYKSMDRVIESHLRGAEILYSLAIANARHAGMEGRYPISDYGLLVDARRSVGLFQHHDAITGTAKENVVIDYGNKLLRSLIGLKRVIINAAHFLVMKNKEFYRFYQTEPFLETDDRRATQDSLPQRTLIELDPAGPRYLVVFNPIEQERLCTVTVLVNTVKVRVLTEDGQTLPVQLSAQWSSASQMSAEVFEATFMVRLPPLGLAVFHLYESPDSPMTLRSETLLRLSSRGVAARAVDPLPVRSQQSDPQTFYISSQSLTLGFSGTTGLLESIKRKDDSQEVKVQMQFMMYGTRPSKDKSGAYLFLPDGKAKPYNQKEPPVVRVVEGPLFSEVVAYYQHFQQSIRIYNVPGVDGFSVDVATAVDIRDQNNKELSMRLVTNIQSGDLFYTDLNGFQIQPRRHHLKLPLQANFYPMPSQAYIQDSNYRLTMHTAQALGVSSLESGQLEVILDRRLMQDDNRGLGQGLKDNKKTINRFRLLLERRSMSNKMDSTSSSFPSILSHMTSSFLNLDVLALPVIPKRRGVPPLQTFAPLKSILPCDFRLVNLRSIQTQQDPQSPSPYTALILHRLPLDCGLETPNLGFNCTTTQGQLTVSRLFKNLDLQLLQPMSLTLMYSSPPLANESTVTLDPMEITTFKLKLR

Target ORF information:

RefSeq Version	XM_029842068.1
Organism	Takifugu rubripes(torafugu)
Definition	Takifugu rubripes mannosidase alpha class 2A member 2 (man2a2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_029842068.1

ORF Insert Sequence:

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTAGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACAC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTTCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
GACAGCACAT CGTCCAGCTT CCCCTCCATT CTCAGTCACA TGACCAGCTC CTTCCTGAAC 
CTAGACGTCT TGGCGCTGCC TGTAATTCCC AAAAGACGCG GCGTCCCTCC TCTGCAGACC 
TTCGCCCCTC TCAAGTCCAT CCTGCCCTGT GACTTTCGCT TGGTCAACCT GCGCAGCATC 
CAGACCCAGC AGGACCCCCA ATCTCCATCT CCGTACACAG CCTTGATTCT TCACCGTCTG 
CCGTTGGATT GTGGCCTGGA GACTCCGAAC CTGGGCTTTA ACTGCACTAC CACACAGGGG 
CAGCTAACTG TATCGAGACT GTTCAAGAAT CTGGACCTGC AGCTCCTCCA GCCCATGTCC 
CTGACTCTGA TGTACTCCAG CCCACCTCTG GCCAATGAAT CCACCGTCAC CCTGGATCCC 
ATGGAGATCA CCACCTTCAA GCTCAAACTA CGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_011607291.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605593.1 Takifugu rubripes mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X1, mRNA.

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CloneID	OTo19125
Clone ID Related Accession (Same CDS sequence)	XM_011607291.1 , XM_011607294.1 , XM_011607292.1 , XM_011607293.1
Accession Version	XM_011607291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	alpha-mannosidase 2x
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071015.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTGGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACTC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTGCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605593.1
CDS	841..4299
Translation	MKLRKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQSGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVMELTDTGAVSPSGHLPFRSANGSWVLPFDGRPTFLALKPQDCQFTAGSHGQADVQMLDVYSLLKFDNPDGGVWKQGFDITYEPDEWDREPLQVFVVPHSHNDPGWIKTFDKYFMEQTQHILNNMVVKLLEDPRRKFIWSEISFFAKWWETADTYKQEAARKLILGGQLEIVTGGWVMTDEANVHYFAMLDQLIEGHQWLERNLGVTPRSAWAVDPFGHSATMPYMLKRSNLTSMLIQRVHYSIKKHFASTRSLEFMWRQGWDTGSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPKTVVEANVAERANLLLDQYRKKSKLYRSKVLLVPLGDDFRYDKALEWDQQYTNYQKLFDYMNSHPELHVQAQFGTLSDYFNAVYKAHGVAQGSRPADYPVLSGDFFAYADREDHYWTGYFTSRPFYKSMDRVIESHLRGAEILYSLAIANARHAGMEGRYPISDYGLLVDARRSVGLFQHHDAITGTAKENVVIDYGNKLLRSLIGLKRVIINAAHFLVMKNKEFYRFYQTEPFLETDDRRATQDSLPQRTLIELDPAGPRYLVVFNPIEQERLCTVTVLVNTVKVRVLTEDGQTLPVQLSAQWSSASQMSAEVFEATFMVRLPPLGLAVFHLYESPDSPMTLRSETLLRLSSRGVAARAVDPLPVRSQQSDPQTFYISSQSLTLGFSGTTGLLESIKRKDDSQEVKVQMQFMMYGTRPSKDKSGAYLFLPDGKAKPYNQKEPPVVRVVEGPLFSEVVAYYQHFQQSIRIYNVPGVDGFSVDVATAVDIRDQNNKELSMRLVTNIQSGDLFYTDLNGFQIQPRRHHLKLPLQANFYPMPSQAYIQDSNYRLTMHTAQALGVSSLESGQLEVILDRRLMQDDNRGLGQGLKDNKKTINRFRLLLERRSMSNKMMDSTSSSFPSILSHMTSSFLNLDVLALPVIPKRRGVPPLQTFAPLKSILPCDFRLVNLRSIQTQQDPQSPSPYTALILHRLPLDCGLETPNLGFNCTTTQGQLTVSRLFKNLDLQLLQPMSLTLMYSSPPLANESTVTLDPMEITTFKLKLR

Target ORF information:

RefSeq Version	XM_011607291.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607291.1

ORF Insert Sequence:

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTGGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACTC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTGCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_011607294.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605596.1 Takifugu rubripes mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OTo19125
Clone ID Related Accession (Same CDS sequence)	XM_011607291.1 , XM_011607294.1 , XM_011607292.1 , XM_011607293.1
Accession Version	XM_011607294.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	alpha-mannosidase 2x
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071015.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTGGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACTC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTGCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605596.1
CDS	47..3505
Translation	MKLRKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQSGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVMELTDTGAVSPSGHLPFRSANGSWVLPFDGRPTFLALKPQDCQFTAGSHGQADVQMLDVYSLLKFDNPDGGVWKQGFDITYEPDEWDREPLQVFVVPHSHNDPGWIKTFDKYFMEQTQHILNNMVVKLLEDPRRKFIWSEISFFAKWWETADTYKQEAARKLILGGQLEIVTGGWVMTDEANVHYFAMLDQLIEGHQWLERNLGVTPRSAWAVDPFGHSATMPYMLKRSNLTSMLIQRVHYSIKKHFASTRSLEFMWRQGWDTGSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPKTVVEANVAERANLLLDQYRKKSKLYRSKVLLVPLGDDFRYDKALEWDQQYTNYQKLFDYMNSHPELHVQAQFGTLSDYFNAVYKAHGVAQGSRPADYPVLSGDFFAYADREDHYWTGYFTSRPFYKSMDRVIESHLRGAEILYSLAIANARHAGMEGRYPISDYGLLVDARRSVGLFQHHDAITGTAKENVVIDYGNKLLRSLIGLKRVIINAAHFLVMKNKEFYRFYQTEPFLETDDRRATQDSLPQRTLIELDPAGPRYLVVFNPIEQERLCTVTVLVNTVKVRVLTEDGQTLPVQLSAQWSSASQMSAEVFEATFMVRLPPLGLAVFHLYESPDSPMTLRSETLLRLSSRGVAARAVDPLPVRSQQSDPQTFYISSQSLTLGFSGTTGLLESIKRKDDSQEVKVQMQFMMYGTRPSKDKSGAYLFLPDGKAKPYNQKEPPVVRVVEGPLFSEVVAYYQHFQQSIRIYNVPGVDGFSVDVATAVDIRDQNNKELSMRLVTNIQSGDLFYTDLNGFQIQPRRHHLKLPLQANFYPMPSQAYIQDSNYRLTMHTAQALGVSSLESGQLEVILDRRLMQDDNRGLGQGLKDNKKTINRFRLLLERRSMSNKMMDSTSSSFPSILSHMTSSFLNLDVLALPVIPKRRGVPPLQTFAPLKSILPCDFRLVNLRSIQTQQDPQSPSPYTALILHRLPLDCGLETPNLGFNCTTTQGQLTVSRLFKNLDLQLLQPMSLTLMYSSPPLANESTVTLDPMEITTFKLKLR

Target ORF information:

RefSeq Version	XM_011607294.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607294.1

ORF Insert Sequence:

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTGGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACTC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTGCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_011607292.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605594.1 Takifugu rubripes mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OTo19125
Clone ID Related Accession (Same CDS sequence)	XM_011607291.1 , XM_011607294.1 , XM_011607292.1 , XM_011607293.1
Accession Version	XM_011607292.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	alpha-mannosidase 2x
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071015.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTGGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACTC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTGCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605594.1
CDS	760..4218
Translation	MKLRKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQSGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVMELTDTGAVSPSGHLPFRSANGSWVLPFDGRPTFLALKPQDCQFTAGSHGQADVQMLDVYSLLKFDNPDGGVWKQGFDITYEPDEWDREPLQVFVVPHSHNDPGWIKTFDKYFMEQTQHILNNMVVKLLEDPRRKFIWSEISFFAKWWETADTYKQEAARKLILGGQLEIVTGGWVMTDEANVHYFAMLDQLIEGHQWLERNLGVTPRSAWAVDPFGHSATMPYMLKRSNLTSMLIQRVHYSIKKHFASTRSLEFMWRQGWDTGSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPKTVVEANVAERANLLLDQYRKKSKLYRSKVLLVPLGDDFRYDKALEWDQQYTNYQKLFDYMNSHPELHVQAQFGTLSDYFNAVYKAHGVAQGSRPADYPVLSGDFFAYADREDHYWTGYFTSRPFYKSMDRVIESHLRGAEILYSLAIANARHAGMEGRYPISDYGLLVDARRSVGLFQHHDAITGTAKENVVIDYGNKLLRSLIGLKRVIINAAHFLVMKNKEFYRFYQTEPFLETDDRRATQDSLPQRTLIELDPAGPRYLVVFNPIEQERLCTVTVLVNTVKVRVLTEDGQTLPVQLSAQWSSASQMSAEVFEATFMVRLPPLGLAVFHLYESPDSPMTLRSETLLRLSSRGVAARAVDPLPVRSQQSDPQTFYISSQSLTLGFSGTTGLLESIKRKDDSQEVKVQMQFMMYGTRPSKDKSGAYLFLPDGKAKPYNQKEPPVVRVVEGPLFSEVVAYYQHFQQSIRIYNVPGVDGFSVDVATAVDIRDQNNKELSMRLVTNIQSGDLFYTDLNGFQIQPRRHHLKLPLQANFYPMPSQAYIQDSNYRLTMHTAQALGVSSLESGQLEVILDRRLMQDDNRGLGQGLKDNKKTINRFRLLLERRSMSNKMMDSTSSSFPSILSHMTSSFLNLDVLALPVIPKRRGVPPLQTFAPLKSILPCDFRLVNLRSIQTQQDPQSPSPYTALILHRLPLDCGLETPNLGFNCTTTQGQLTVSRLFKNLDLQLLQPMSLTLMYSSPPLANESTVTLDPMEITTFKLKLR

Target ORF information:

RefSeq Version	XM_011607292.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607292.1

ORF Insert Sequence:

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTGGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACTC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTGCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

man2a2 ( XM_011607293.1 ) cDNA ORF clone, Takifugu rubripes(torafugu) -> XP_011605595.1 Takifugu rubripes mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X3, mRNA.

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CloneID	OTo19125
Clone ID Related Accession (Same CDS sequence)	XM_011607291.1 , XM_011607294.1 , XM_011607292.1 , XM_011607293.1
Accession Version	XM_011607293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-15
Organism	Takifugu rubripes(Fugu rubripes)
Product	alpha-mannosidase 2x
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004071015.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Takifugu rubripes Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTGGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACTC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTGCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011605595.1
CDS	172..3630
Translation	MKLRKQVTVCGGAIFCVAVFSLYLMLDRVQHDPARRQSGGNFPRSQISVLQNRIEQLEQLLEENHQIISHIKDSVMELTDTGAVSPSGHLPFRSANGSWVLPFDGRPTFLALKPQDCQFTAGSHGQADVQMLDVYSLLKFDNPDGGVWKQGFDITYEPDEWDREPLQVFVVPHSHNDPGWIKTFDKYFMEQTQHILNNMVVKLLEDPRRKFIWSEISFFAKWWETADTYKQEAARKLILGGQLEIVTGGWVMTDEANVHYFAMLDQLIEGHQWLERNLGVTPRSAWAVDPFGHSATMPYMLKRSNLTSMLIQRVHYSIKKHFASTRSLEFMWRQGWDTGSSTDIFCHMMPFYSYDVPHTCGPDPKICCQFDFKRLPGGRINCPWKVPPKTVVEANVAERANLLLDQYRKKSKLYRSKVLLVPLGDDFRYDKALEWDQQYTNYQKLFDYMNSHPELHVQAQFGTLSDYFNAVYKAHGVAQGSRPADYPVLSGDFFAYADREDHYWTGYFTSRPFYKSMDRVIESHLRGAEILYSLAIANARHAGMEGRYPISDYGLLVDARRSVGLFQHHDAITGTAKENVVIDYGNKLLRSLIGLKRVIINAAHFLVMKNKEFYRFYQTEPFLETDDRRATQDSLPQRTLIELDPAGPRYLVVFNPIEQERLCTVTVLVNTVKVRVLTEDGQTLPVQLSAQWSSASQMSAEVFEATFMVRLPPLGLAVFHLYESPDSPMTLRSETLLRLSSRGVAARAVDPLPVRSQQSDPQTFYISSQSLTLGFSGTTGLLESIKRKDDSQEVKVQMQFMMYGTRPSKDKSGAYLFLPDGKAKPYNQKEPPVVRVVEGPLFSEVVAYYQHFQQSIRIYNVPGVDGFSVDVATAVDIRDQNNKELSMRLVTNIQSGDLFYTDLNGFQIQPRRHHLKLPLQANFYPMPSQAYIQDSNYRLTMHTAQALGVSSLESGQLEVILDRRLMQDDNRGLGQGLKDNKKTINRFRLLLERRSMSNKMMDSTSSSFPSILSHMTSSFLNLDVLALPVIPKRRGVPPLQTFAPLKSILPCDFRLVNLRSIQTQQDPQSPSPYTALILHRLPLDCGLETPNLGFNCTTTQGQLTVSRLFKNLDLQLLQPMSLTLMYSSPPLANESTVTLDPMEITTFKLKLR

Target ORF information:

RefSeq Version	XM_011607293.1
Organism	Takifugu rubripes(Fugu rubripes)
Definition	Takifugu rubripes mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011607293.1

ORF Insert Sequence:

ATGAAGCTGA GGAAACAGGT GACGGTGTGT GGAGGGGCAA TATTCTGTGT GGCTGTGTTC 
TCACTCTATC TGATGTTGGA TCGAGTCCAG CATGACCCTG CACGGAGACA GAGTGGTGGA 
AACTTTCCCC GGAGCCAAAT CTCAGTTCTG CAAAATCGAA TAGAGCAGCT GGAGCAGCTT 
CTGGAGGAAA ATCACCAGAT CATCAGCCAC ATAAAGGACT CTGTGATGGA GCTCACAGAC 
ACAGGAGCTG TGTCTCCCAG TGGCCACCTG CCGTTCAGAA GTGCCAATGG TTCCTGGGTC 
TTGCCCTTTG ACGGCCGGCC CACTTTCCTT GCTCTGAAGC CCCAGGATTG TCAGTTTACT 
GCAGGCAGCC ATGGACAAGC TGATGTCCAG ATGCTGGATG TGTACTCTCT CCTCAAGTTT 
GATAACCCCG ACGGTGGTGT GTGGAAGCAG GGCTTTGACA TAACGTATGA ACCCGATGAG 
TGGGACAGAG AACCTCTGCA AGTGTTTGTG GTTCCACACT CCCACAATGA TCCAGGCTGG 
ATCAAGACTT TTGACAAGTA TTTCATGGAG CAGACGCAGC ACATTTTAAA CAACATGGTG 
GTGAAGCTGC TTGAGGATCC ACGCAGGAAG TTTATCTGGT CCGAAATTTC ATTCTTCGCC 
AAGTGGTGGG AAACTGCAGA TACGTACAAG CAGGAGGCGG CACGCAAACT CATCCTTGGA 
GGACAGCTAG AGATTGTAAC AGGAGGTTGG GTGATGACTG ATGAAGCCAA TGTTCACTAC 
TTTGCCATGT TAGACCAGCT CATTGAAGGA CATCAATGGC TAGAAAGAAA CCTGGGTGTA 
ACCCCTCGCA GTGCCTGGGC AGTCGACCCT TTTGGTCACA GCGCTACTAT GCCCTATATG 
CTGAAGAGGT CCAACCTGAC CAGCATGCTA ATCCAGAGGG TTCACTACTC TATCAAAAAG 
CACTTTGCCT CCACCCGTAG CTTGGAGTTC ATGTGGAGGC AGGGCTGGGA CACGGGATCG 
AGCACAGACA TATTCTGCCA CATGATGCCG TTCTACAGCT ACGACGTGCC TCACACGTGC 
GGCCCCGACC CCAAGATCTG CTGCCAGTTT GATTTCAAGA GGTTGCCGGG TGGTCGAATC 
AACTGTCCCT GGAAGGTGCC CCCGAAAACT GTAGTGGAGG CAAATGTGGC AGAGAGGGCT 
AATCTGCTGC TGGACCAGTA CCGCAAAAAG TCCAAACTGT ACCGGAGCAA GGTCCTCCTG 
GTCCCCCTGG GAGATGACTT CCGGTATGAC AAGGCCCTGG AGTGGGATCA GCAATACACC 
AACTACCAGA AGCTCTTTGA CTACATGAAT TCTCATCCAG AGCTACATGT ACAAGCCCAG 
TTTGGCACTC TCTCAGACTA TTTCAACGCC GTGTACAAAG CACACGGCGT GGCCCAGGGA 
TCCAGGCCCG CTGACTACCC AGTTCTGAGT GGAGATTTCT TCGCCTATGC AGATCGTGAG 
GACCACTACT GGACGGGTTA TTTCACCTCC CGGCCCTTTT ACAAGAGCAT GGACCGTGTG 
ATCGAGTCAC ATCTCAGGGG GGCTGAGATC CTCTACAGCT TGGCAATTGC AAATGCTCGC 
CACGCTGGGA TGGAAGGACG CTACCCGATA TCAGATTATG GTCTGTTGGT AGATGCCAGA 
CGGTCTGTTG GCCTGTTCCA GCATCACGAT GCCATAACTG GCACAGCAAA GGAGAATGTT 
GTCATCGACT ACGGTAACAA ATTGCTGCGC TCCCTCATCG GGCTGAAAAG AGTAATCATC 
AATGCTGCTC ATTTCCTGGT GATGAAGAAC AAAGAGTTTT ATCGCTTTTA CCAAACGGAG 
CCCTTCCTGG AGACGGATGA CAGACGCGCT ACCCAAGACT CTCTGCCTCA GCGCACTCTA 
ATTGAACTGG ACCCAGCAGG GCCGCGATAC CTGGTTGTGT TCAACCCCAT TGAGCAGGAG 
CGTCTCTGCA CGGTAACGGT CCTGGTGAAC ACGGTGAAGG TGCGAGTGCT TACGGAGGAT 
GGACAGACTC TTCCTGTACA GCTGAGCGCT CAGTGGAGCT CTGCTAGTCA GATGAGCGCT 
GAGGTGTTTG AGGCAACATT CATGGTTCGT CTGCCCCCTC TGGGTCTGGC CGTCTTCCAC 
CTCTATGAAT CTCCGGACTC GCCGATGACT CTCCGCTCCG AGACCCTGCT CAGGCTGTCG 
AGTCGAGGAG TAGCAGCTCG AGCTGTGGAC CCGCTTCCTG TCCGCTCGCA GCAGTCTGAC 
CCTCAGACCT TCTACATCAG CAGTCAGTCT CTCACTCTGG GCTTCTCTGG AACTACCGGC 
CTACTGGAGA GCATCAAGCG GAAGGACGAC TCCCAGGAAG TGAAGGTTCA GATGCAGTTC 
ATGATGTACG GCACTCGTCC CTCTAAAGAC AAAAGTGGAG CTTACCTTTT CCTGCCTGAT 
GGAAAAGCAA AGCCCTACAA TCAGAAAGAG CCCCCTGTGG TACGCGTGGT GGAAGGGCCT 
CTCTTCTCTG AGGTGGTGGC CTACTATCAG CATTTCCAGC AGTCCATTCG CATATACAAT 
GTGCCAGGGG TGGATGGATT TTCCGTAGAC GTCGCCACCG CCGTGGACAT CAGAGATCAA 
AACAATAAGG AGCTGTCGAT GCGTCTGGTG ACGAACATCC AGAGTGGAGA CCTCTTCTAC 
ACCGATCTGA ATGGTTTCCA GATACAGCCC CGCCGGCACC ACCTGAAGCT CCCCCTGCAG 
GCCAACTTCT ACCCCATGCC CAGTCAGGCC TACATCCAGG ACAGCAACTA CCGCCTCACG 
ATGCACACGG CTCAGGCTCT GGGAGTCAGC AGCCTGGAGA GCGGCCAGCT GGAAGTGATC 
CTGGACCGGC GGCTGATGCA GGATGACAAT CGCGGGCTGG GTCAGGGCCT GAAGGACAAC 
AAGAAGACAA TCAACCGCTT CCGCCTGCTG CTGGAAAGGA GATCGATGAG CAATAAGATG 
ATGGACAGCA CATCGTCCAG CTTCCCCTCC ATTCTCAGTC ACATGACCAG CTCCTTCCTG 
AACCTAGACG TCTTGGCGCT GCCTGTAATT CCCAAAAGAC GCGGCGTCCC TCCTCTGCAG 
ACCTTCGCCC CTCTCAAGTC CATCCTGCCC TGTGACTTTC GCTTGGTCAA CCTGCGCAGC 
ATCCAGACCC AGCAGGACCC CCAATCTCCA TCTCCGTACA CAGCCTTGAT TCTTCACCGT 
CTGCCGTTGG ATTGTGGCCT GGAGACTCCG AACCTGGGCT TTAACTGCAC TACCACACAG 
GGGCAGCTAA CTGTATCGAG ACTGTTCAAG AATCTGGACC TGCAGCTCCT CCAGCCCATG 
TCCCTGACTC TGATGTACTC CAGCCCACCT CTGGCCAATG AATCCACCGT CACCCTGGAT 
CCCATGGAGA TCACCACCTT CAAGCTCAAA CTACGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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man2a2 cDNA ORF clone, Takifugu rubripes(torafugu)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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FAX

オンラインフォーム