FOXO1 cDNA ORF clone, Sus scrofa(pig)

Gene
Clones
gRNAs

Summary

Gene Symbol	FOXO1
Entrez Gene ID	397077
Full Name	forkhead box O1
Synonyms	FOXO1A
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 11 Map Location: 11\|11

mRNA and Protein(s)

mRNA	Protein	Name
XM_021065269.1	XP_020920928.1	forkhead box protein O1
NM_214014.2	NP_999179.2	forkhead box protein O1
NM_214014.3	NP_999179.3	forkhead box protein O1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

PDGFR? Regulated by miR-34a and FoxO1 Promotes Adipogenesis in Porcine Intramuscular Preadipocytes through Erk Signaling Pathway.
Sun YM, Qin J, Liu SG, Cai R, Chen XC, Wang XM, Pang WJ
International journal of molecular sciences18(11)(2017 Nov)

Effects of MicroRNA-27a on Myogenin Expression and Akt/FoxO1 Signal Pathway during Porcine Myoblast Differentiation.
Zhang S, Chen X, Huang Z, Chen D, Yu B, He J, Zheng P, Yu J, Luo J, Luo Y, Chen H
Animal biotechnology1-7(2017 Aug)

FOXO1-suppressed miR-424 regulates the proliferation and osteogenic differentiation of MSCs by targeting FGF2 under oxidative stress.
Li L, Qi Q, Luo J, Huang S, Ling Z, Gao M, Zhou Z, Stiehler M, Zou X
Scientific reports742331(2017 Feb)

Glycine enhances muscle protein mass associated with maintaining Akt-mTOR-FOXO1 signaling and suppressing TLR4 and NOD2 signaling in piglets challenged with LPS.
Liu Y, Wang X, Wu H, Chen S, Zhu H, Zhang J, Hou Y, Hu CA, Zhang G
American journal of physiology. Regulatory, integrative and comparative physiology311(2)R365-73(2016 Aug)

Let-7g induces granulosa cell apoptosis by targeting MAP3K1 in the porcine ovary.
Cao R, Wu W, Zhou X, Liu K, Li B, Huang X, Zhang Y, Liu H
The international journal of biochemistry & cell biology68148-57(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results indicate glycine enhances muscle protein mass under an inflammatory condition. The beneficial roles of glycine on the muscle are closely associated with maintaining Akt-mTOR-FOXO1 signaling and suppressing the activation of TLR4 and/or NOD2 signaling pathways.
Title: Glycine enhances muscle protein mass associated with maintaining Akt-mTOR-FOXO1 signaling and suppressing TLR4 and NOD2 signaling in piglets challenged with LPS.

let-7g induces porcine granulosa cells apoptosis by inhibiting the MAP3K1 gene, which promotes FoxO1 expression and dephosphorylation with nuclear accumulation.
Title: Let-7g induces granulosa cell apoptosis by targeting MAP3K1 in the porcine ovary.

Results show that FoxO1 likely regulates MyHC I negatively and MyHC IIx and MyHC IIb positively and may play a pivotal role in the determination of muscle fiber type.
Title: Correlation of forkhead box transcription factor O1 and myosin heavy chain isoforms in porcine skeletal muscle.

Data suggest forkhead box protein O1 (FoxO1) involvement in the regulation of TNF-related apoptosis-inducing ligand TRAIL and Fas ligand FasL expression during follicular atresia.
Title: Changes in the expression of Fox O1 and death ligand genes during follicular atresia in porcine ovary.

inhibition of FoxO1 expression level caused by miR-15a/b over-expression had a positive effect on adipogenesis. Thus, we conclude that miR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1
Title: MiR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1.

The following FOXO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FOXO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe00606	NM_214014.2 Latest version!	Sus scrofa forkhead box O1 (FOXO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OSe232551	NM_214014.3 Latest version!	Sus scrofa forkhead box O1 (FOXO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OSe232551	XM_021065269.1 Latest version!	Sus scrofa forkhead box O1 (FOXO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FOXO1 ( NM_214014.2 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999179.2 Sus scrofa forkhead box O1 (FOXO1), mRNA.

Price & Availability↑

CloneID	OSe00606
Clone ID Related Accession (Same CDS sequence)	NM_214014.2
Accession Version	NM_214014.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-29
Organism	Sus scrofa(pig)
Product	forkhead box protein O1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF453379.1. On Apr 15, 2007 this sequence version replaced NM_214014.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF453379.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1876824, SAMEA1876826 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGAGG CGCCACAGGT GGTGGAGATC GACCCGGACT TCGAGCCGCT GCCCCGGCCG 
CGCTCGTGCA CCTGGCCGCT GCCCAGGCCG GAGTTTAGCC AGTCAAACTC GGCCACCTCC 
AGCCCGGCGC CGTCGGGCGG CGCAGCTGCC AACCCCGACG CTGCGGCGGG CCTGCCCTCG 
GCCTCGGCTG CCGCTGTCAA CGCCGACTTC ATGAGCAACC TGAGCTTGCT GGAGGAGAGC 
GAGGACTTCC CGCAGGCGCC CGGCTCCGTG GCGGCGGCGG CGGCGGCGGC AGCCGCGGTG 
GCGGCGGCGG CCGCCGCTGC TGCCACCGGG GGGCTGTGCG GGGACTTCCA GGGCCCGGAG 
GCGGGCTGCC TGCACCCAGC GCCACCGCAG CAGCCCCCGC CGCCCGGGCC GCTGTCGCAG 
CACCCGCCCG TGCCCCCCGC CGCCGCTGGG TCGCTTGCGG GGCAGCCGCG CAAGAGCAGC 
TCGTCCCGCC GCAACGCGTG GGGCAACCTG TCCTACGCCG ACCTCATCAC CAAGGCCATC 
GAGAGCTCGG CGGAGAAGCG GCTCACGCTC TCGCAGATCT ACGAGTGGAT GGTCAAGAGC 
GTGCCCTACT TCAAGGATAA GGGTGACAGC AACAGCTCGG CGGGCTGGAA GAATTCAATT 
CGTCATAATC TGTCCCTACA CAGCAAGTTC ATTCGTGTGC AGAATGAAGG AACTGGAAAA 
AGTTCCTGGT GGATGCTCAA CCCAGAGGGT GGCAAGAGTG GGAAATCTCC CAGGAGAAGA 
GCCGCCTCCA TGGACAACAA CAGTAAATTT GCCAAGAGCC GAGGCCGGGC TGCCAAGAAA 
AAAGCATCAC TCCAGTCTGG CCAGGAGGGT GCAGGGGACA GCCCGGGATC TCAGTTTTCG 
AAGTGGCCCG CCAGCCCTGG CTCTCACAGC AACGATGACT TTGATAACTG GAGTACATTT 
CGCCCACGGA CTAGCTCCAA TGCTAGTACT ATTAGTGGGA GGCTCTCTCC CATCATGACT 
GAGCAGGATG ACCTTGGAAA CGGGGATGTG CATTCTATGG TGTACCCACC CTCGGCGGCA 
AAGATGGCTT CTACTCTGCC CAGTCTGTCT GAGATAAGCA ATCCTGAGAA CATGGAGAAC 
CTTTTGGATA ATCTCAACCT TCTCTCATCA CCAACGTCAT TAACTGTTTC CACCCAGTCT 
TCACCAGGCA CCATCATGCA GCAGACGCCA TGCTACTCAT TTGCGCCACC AAACACCAGT 
CTGAATTCAC CCAGCCCCAA CTACCAAAAA TACACGTATG GCCAATCCAG CATGAGCCCT 
TTGCCCCAGA TGCCTATGCA AACACTTCAG GACAGCAAAT CGAGTTACGG AGGCATGGCC 
CAGTATAACT GTGCAGCGGG ACTCTTGAAG GAGTTACTTA CTTCCGACTC TCCTCCCCAT 
AATGACATTA TGACGCCAGT CGATCCGGGG GTGGCCCAAC CCAACAGCCG AGTCCTTGGC 
CAGAATGTGC TGATGGGCCC TAGTTCGGTC ATGCCAGCCT ACGGCGGCCA GGCCTCTCAT 
AACAAAATGA TGAATCCCAG CTCCCACAGC CACCCTGGAC ACGCTCAGTC CACGTCTGCC 
GTCAATGGGC GTGCCTTGCC CCACGCGGTG AACACCATGC CCCACGCCTC GGGGATGAAC 
CGCCTGACCC AGGAGAAGAC CGCTTTACAA GTGCCTCTGC CCCACCCCAT GCAGATGAAC 
GCCCTGGGGG GCTACTCCCC CGCCAGCACC TGCAACGGGT ATGGCAGGAT GGGCCTTCTC 
CACCAGGAGA AGCTCCCGAG TGACTTGGAT GGCATGTTCA TTGAGCGCTT AGACTGTGAC 
ATGGAGTCCA TCATTCGGAA CGACCTCATG GATGGAGATA CATTGGATTT TAACTTTGAC 
AATGTGTTGC CCAACCAAAG CTTCCCACAC AGTGTCAAGA CAACGACACA TAGCTGGGTG 
TCAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999179.2
CDS	206..2194
Translation	MAEAPQVVEIDPDFEPLPRPRSCTWPLPRPEFSQSNSATSSPAPSGGAAANPDAAAGLPSASAAAVNADFMSNLSLLEESEDFPQAPGSVAAAAAAAAAVAAAAAAAATGGLCGDFQGPEAGCLHPAPPQQPPPPGPLSQHPPVPPAAAGSLAGQPRKSSSSRRNAWGNLSYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAASMDNNSKFAKSRGRAAKKKASLQSGQEGAGDSPGSQFSKWPASPGSHSNDDFDNWSTFRPRTSSNASTISGRLSPIMTEQDDLGNGDVHSMVYPPSAAKMASTLPSLSEISNPENMENLLDNLNLLSSPTSLTVSTQSSPGTIMQQTPCYSFAPPNTSLNSPSPNYQKYTYGQSSMSPLPQMPMQTLQDSKSSYGGMAQYNCAAGLLKELLTSDSPPHNDIMTPVDPGVAQPNSRVLGQNVLMGPSSVMPAYGGQASHNKMMNPSSHSHPGHAQSTSAVNGRALPHAVNTMPHASGMNRLTQEKTALQVPLPHPMQMNALGGYSPASTCNGYGRMGLLHQEKLPSDLDGMFIERLDCDMESIIRNDLMDGDTLDFNFDNVLPNQSFPHSVKTTTHSWVSG

Target ORF information:

RefSeq Version	NM_214014.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa forkhead box O1 (FOXO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214014.2

ORF Insert Sequence:

ATGGCCGAGG CGCCACAGGT GGTGGAGATC GACCCGGACT TCGAGCCGCT GCCCCGGCCG 
CGCTCGTGCA CCTGGCCGCT GCCCAGGCCG GAGTTTAGCC AGTCAAACTC GGCCACCTCC 
AGCCCGGCGC CGTCGGGCGG CGCAGCTGCC AACCCCGACG CTGCGGCGGG CCTGCCCTCG 
GCCTCGGCTG CCGCTGTCAA CGCCGACTTC ATGAGCAACC TGAGCTTGCT GGAGGAGAGC 
GAGGACTTCC CGCAGGCGCC CGGCTCCGTG GCGGCGGCGG CGGCGGCGGC AGCCGCGGTG 
GCGGCGGCGG CCGCCGCTGC TGCCACCGGG GGGCTGTGCG GGGACTTCCA GGGCCCGGAG 
GCGGGCTGCC TGCACCCAGC GCCACCGCAG CAGCCCCCGC CGCCCGGGCC GCTGTCGCAG 
CACCCGCCCG TGCCCCCCGC CGCCGCTGGG TCGCTTGCGG GGCAGCCGCG CAAGAGCAGC 
TCGTCCCGCC GCAACGCGTG GGGCAACCTG TCCTACGCCG ACCTCATCAC CAAGGCCATC 
GAGAGCTCGG CGGAGAAGCG GCTCACGCTC TCGCAGATCT ACGAGTGGAT GGTCAAGAGC 
GTGCCCTACT TCAAGGATAA GGGTGACAGC AACAGCTCGG CGGGCTGGAA GAATTCAATT 
CGTCATAATC TGTCCCTACA CAGCAAGTTC ATTCGTGTGC AGAATGAAGG AACTGGAAAA 
AGTTCCTGGT GGATGCTCAA CCCAGAGGGT GGCAAGAGTG GGAAATCTCC CAGGAGAAGA 
GCCGCCTCCA TGGACAACAA CAGTAAATTT GCCAAGAGCC GAGGCCGGGC TGCCAAGAAA 
AAAGCATCAC TCCAGTCTGG CCAGGAGGGT GCAGGGGACA GCCCGGGATC TCAGTTTTCG 
AAGTGGCCCG CCAGCCCTGG CTCTCACAGC AACGATGACT TTGATAACTG GAGTACATTT 
CGCCCACGGA CTAGCTCCAA TGCTAGTACT ATTAGTGGGA GGCTCTCTCC CATCATGACT 
GAGCAGGATG ACCTTGGAAA CGGGGATGTG CATTCTATGG TGTACCCACC CTCGGCGGCA 
AAGATGGCTT CTACTCTGCC CAGTCTGTCT GAGATAAGCA ATCCTGAGAA CATGGAGAAC 
CTTTTGGATA ATCTCAACCT TCTCTCATCA CCAACGTCAT TAACTGTTTC CACCCAGTCT 
TCACCAGGCA CCATCATGCA GCAGACGCCA TGCTACTCAT TTGCGCCACC AAACACCAGT 
CTGAATTCAC CCAGCCCCAA CTACCAAAAA TACACGTATG GCCAATCCAG CATGAGCCCT 
TTGCCCCAGA TGCCTATGCA AACACTTCAG GACAGCAAAT CGAGTTACGG AGGCATGGCC 
CAGTATAACT GTGCAGCGGG ACTCTTGAAG GAGTTACTTA CTTCCGACTC TCCTCCCCAT 
AATGACATTA TGACGCCAGT CGATCCGGGG GTGGCCCAAC CCAACAGCCG AGTCCTTGGC 
CAGAATGTGC TGATGGGCCC TAGTTCGGTC ATGCCAGCCT ACGGCGGCCA GGCCTCTCAT 
AACAAAATGA TGAATCCCAG CTCCCACAGC CACCCTGGAC ACGCTCAGTC CACGTCTGCC 
GTCAATGGGC GTGCCTTGCC CCACGCGGTG AACACCATGC CCCACGCCTC GGGGATGAAC 
CGCCTGACCC AGGAGAAGAC CGCTTTACAA GTGCCTCTGC CCCACCCCAT GCAGATGAAC 
GCCCTGGGGG GCTACTCCCC CGCCAGCACC TGCAACGGGT ATGGCAGGAT GGGCCTTCTC 
CACCAGGAGA AGCTCCCGAG TGACTTGGAT GGCATGTTCA TTGAGCGCTT AGACTGTGAC 
ATGGAGTCCA TCATTCGGAA CGACCTCATG GATGGAGATA CATTGGATTT TAACTTTGAC 
AATGTGTTGC CCAACCAAAG CTTCCCACAC AGTGTCAAGA CAACGACACA TAGCTGGGTG 
TCAGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOXO1 ( NM_214014.3 ) cDNA ORF clone, Sus scrofa(pig) -> NP_999179.3 Sus scrofa forkhead box O1 (FOXO1), mRNA.

Price & Availability↑

CloneID	OSe232551
Clone ID Related Accession (Same CDS sequence)	XM_021065269.1 , NM_214014.3
Accession Version	NM_214014.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Sus scrofa(pig)
Product	forkhead box protein O1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX920024.2, AY094061.1, AK234453.1 and AEMK02000075.1. On or before Mar 18, 2018 this sequence version replaced XM_021065269.1, NM_214014.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: EF453379.1, AY094061.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN04484959 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGAAG CGCCCCAGGT GGTGGAGATC GACCCGGACT TCGAGCCGCT GCCCCGGCCG 
CGCTCGTGCA CCTGGCCGCT GCCCAGGCCG GAGTTTAGCC AGTCTAACTC GGCCACCTCC 
AGCCCGGCGC CGTCGGGTGG CCCAGCCGCG AACCCCGACG CTGCCGCGGG CCTGCCCTCG 
GCCTCGGCGG CCGCTGTCAA CGCCGACTTC ATGAGCAACC TGAGCCTGCT GGAGGAGAGC 
GGGGACTTCC AGCAGGCGCC CGGCTCCGTG GCGGCGGCGG CGGCGGCGGC AGCCGCGGTG 
GCGGCGGCGG CCGCCGCTGC TGCCACCGGG GGGTTGTGCG GGGACTTCCA GGGCCCAGAG 
GCGGGCTGCC TGCACCCGGC GCCGCCGCAG CAGCCCCCGC CGCCCGGGCC GCTGTCGCAG 
CACCCGCCTG TGCCCCCCGC CGCCGCCGGG TCGCTTGCCG GGCAGCCGCG CAAGAGCAGC 
TCGTCCCGCC GCAACGCGTG GGGCAACCTG TCCTACGCCG ACCTCATCAC CAAGGCCATC 
GAGAGCTCGG CAGAGAAGCG GCTCACGCTA TCGCAGATCT ACGAGTGGAT GGTCAAGAGC 
GTGCCCTACT TCAAGGATAA GGGCGACAGC AACAGCTCGG CAGGCTGGAA GAATTCAATT 
CGTCATAATC TGTCCCTACA CAGCAAGTTC ATTCGCGTGC AGAATGAAGG AACTGGAAAA 
AGTTCCTGGT GGATGCTCAA CCCAGAGGGA GGCAAGAGTG GGAAATCCCC CAGGAGAAGA 
GCCGCCTCCA TGGACAACAA CAGTAAATTT GCCAAGAGCC GAGGCCGGGC TGCCAAGAAA 
AAAGCATCCC TCCAGTCTGG CCAGGAGGGT GCCGGGGACA GCCCGGGATC TCAGTTTTCG 
AAGTGGCCCG CCAGCCCTGG CTCTCACAGC AACGATGACT TTGATAACTG GAGTACATTT 
CGGCCGCGCA CTAGCTCGAA TGCTAGTACT ATTAGTGGGA GGCTCTCTCC CATCATGACT 
GAGCAGGATG ACCTTGGAGA CGGGGATGTG CATTCTATGG TGTACCCACC CTCGGCGGCA 
AAGATGGCTT CTACTCTGCC CAGTCTGTCT GAGATAAGCA ATCCTGAGAA CATGGAGAAC 
CTTTTGGATA ATCTCAACCT TCTCTCATCA CCAACGTCAT TAACTGTTTC AACCCAGTCT 
TCACCAGGCA CCATCATGCA GCAGACGCCA TGCTACTCAT TTGCGCCACC AAACACCAGT 
CTGAATTCAC CCAGCCCCAA CTACCAAAAA TACACGTATG GCCAATCCAG CATGAGCCCT 
TTGCCCCAGA TGCCTATGCA AACACTTCAG GACAGCAAAT CGAGTTACGG AGGCATGGCC 
CAGTATAACT GTGCAGCGGG ACTCTTGAAG GAGTTACTTA CTTCCGACTC TCCTCCCCAT 
AATGACATTA TGACGCCAGT CGATCCGGGG GTGGCCCAAC CCAACAGCCG AGTCCTTGGC 
CAGAATGTGC TGATGGGCCC TAGTTCGGTC ATGCCAGCCT ACGGCGGCCA GGCCTCTCAT 
AACAAAATGA TGAATCCCAG CTCCCACAGC CACCCTGGAC ACGCTCAGTC CACGTCTGCC 
GTCAATGGGC GTGCCTTGCC CCACGCGGTG AACACCATGC CCCACGCCTC GGGGATGAAC 
CGCCTGGCCC AGGCGAAGAC CGCTTTACAA GTGCCTCTGC CCCACCCCAT GCAGATGAAC 
GCCCTGGGGG GCTACTCCCC CGCCAGCACC TGCAACGGCT ATGGCAGGAT GGGCCTTCTC 
CACCAGGAGA AGCTCCCGAG TGACTTGGAT GGCATGTTCA TTGAGCGCTT GGACTGTGAC 
ATGGAGTCCA TCATCCGGAA CGACCTCATG GATGGAGACA CTTTGGATTT TAACTTTGAC 
AATGTGTTGC CCAACCAAAG CTTCCCACAC AGTGTCAAGA CGACGACACA TAGCTGGGTG 
TCAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_999179.3
CDS	441..2429
Translation	MAEAPQVVEIDPDFEPLPRPRSCTWPLPRPEFSQSNSATSSPAPSGGPAANPDAAAGLPSASAAAVNADFMSNLSLLEESGDFQQAPGSVAAAAAAAAAVAAAAAAAATGGLCGDFQGPEAGCLHPAPPQQPPPPGPLSQHPPVPPAAAGSLAGQPRKSSSSRRNAWGNLSYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAASMDNNSKFAKSRGRAAKKKASLQSGQEGAGDSPGSQFSKWPASPGSHSNDDFDNWSTFRPRTSSNASTISGRLSPIMTEQDDLGDGDVHSMVYPPSAAKMASTLPSLSEISNPENMENLLDNLNLLSSPTSLTVSTQSSPGTIMQQTPCYSFAPPNTSLNSPSPNYQKYTYGQSSMSPLPQMPMQTLQDSKSSYGGMAQYNCAAGLLKELLTSDSPPHNDIMTPVDPGVAQPNSRVLGQNVLMGPSSVMPAYGGQASHNKMMNPSSHSHPGHAQSTSAVNGRALPHAVNTMPHASGMNRLAQAKTALQVPLPHPMQMNALGGYSPASTCNGYGRMGLLHQEKLPSDLDGMFIERLDCDMESIIRNDLMDGDTLDFNFDNVLPNQSFPHSVKTTTHSWVSG

Target ORF information:

RefSeq Version	NM_214014.3
Organism	Sus scrofa(pig)
Definition	Sus scrofa forkhead box O1 (FOXO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_214014.3

ORF Insert Sequence:

ATGGCCGAAG CGCCCCAGGT GGTGGAGATC GACCCGGACT TCGAGCCGCT GCCCCGGCCG 
CGCTCGTGCA CCTGGCCGCT GCCCAGGCCG GAGTTTAGCC AGTCTAACTC GGCCACCTCC 
AGCCCGGCGC CGTCGGGTGG CCCAGCCGCG AACCCCGACG CTGCCGCGGG CCTGCCCTCG 
GCCTCGGCGG CCGCTGTCAA CGCCGACTTC ATGAGCAACC TGAGCCTGCT GGAGGAGAGC 
GGGGACTTCC AGCAGGCGCC CGGCTCCGTG GCGGCGGCGG CGGCGGCGGC AGCCGCGGTG 
GCGGCGGCGG CCGCCGCTGC TGCCACCGGG GGGTTGTGCG GGGACTTCCA GGGCCCAGAG 
GCGGGCTGCC TGCACCCGGC GCCGCCGCAG CAGCCCCCGC CGCCCGGGCC GCTGTCGCAG 
CACCCGCCTG TGCCCCCCGC CGCCGCCGGG TCGCTTGCCG GGCAGCCGCG CAAGAGCAGC 
TCGTCCCGCC GCAACGCGTG GGGCAACCTG TCCTACGCCG ACCTCATCAC CAAGGCCATC 
GAGAGCTCGG CAGAGAAGCG GCTCACGCTA TCGCAGATCT ACGAGTGGAT GGTCAAGAGC 
GTGCCCTACT TCAAGGATAA GGGCGACAGC AACAGCTCGG CAGGCTGGAA GAATTCAATT 
CGTCATAATC TGTCCCTACA CAGCAAGTTC ATTCGCGTGC AGAATGAAGG AACTGGAAAA 
AGTTCCTGGT GGATGCTCAA CCCAGAGGGA GGCAAGAGTG GGAAATCCCC CAGGAGAAGA 
GCCGCCTCCA TGGACAACAA CAGTAAATTT GCCAAGAGCC GAGGCCGGGC TGCCAAGAAA 
AAAGCATCCC TCCAGTCTGG CCAGGAGGGT GCCGGGGACA GCCCGGGATC TCAGTTTTCG 
AAGTGGCCCG CCAGCCCTGG CTCTCACAGC AACGATGACT TTGATAACTG GAGTACATTT 
CGGCCGCGCA CTAGCTCGAA TGCTAGTACT ATTAGTGGGA GGCTCTCTCC CATCATGACT 
GAGCAGGATG ACCTTGGAGA CGGGGATGTG CATTCTATGG TGTACCCACC CTCGGCGGCA 
AAGATGGCTT CTACTCTGCC CAGTCTGTCT GAGATAAGCA ATCCTGAGAA CATGGAGAAC 
CTTTTGGATA ATCTCAACCT TCTCTCATCA CCAACGTCAT TAACTGTTTC AACCCAGTCT 
TCACCAGGCA CCATCATGCA GCAGACGCCA TGCTACTCAT TTGCGCCACC AAACACCAGT 
CTGAATTCAC CCAGCCCCAA CTACCAAAAA TACACGTATG GCCAATCCAG CATGAGCCCT 
TTGCCCCAGA TGCCTATGCA AACACTTCAG GACAGCAAAT CGAGTTACGG AGGCATGGCC 
CAGTATAACT GTGCAGCGGG ACTCTTGAAG GAGTTACTTA CTTCCGACTC TCCTCCCCAT 
AATGACATTA TGACGCCAGT CGATCCGGGG GTGGCCCAAC CCAACAGCCG AGTCCTTGGC 
CAGAATGTGC TGATGGGCCC TAGTTCGGTC ATGCCAGCCT ACGGCGGCCA GGCCTCTCAT 
AACAAAATGA TGAATCCCAG CTCCCACAGC CACCCTGGAC ACGCTCAGTC CACGTCTGCC 
GTCAATGGGC GTGCCTTGCC CCACGCGGTG AACACCATGC CCCACGCCTC GGGGATGAAC 
CGCCTGGCCC AGGCGAAGAC CGCTTTACAA GTGCCTCTGC CCCACCCCAT GCAGATGAAC 
GCCCTGGGGG GCTACTCCCC CGCCAGCACC TGCAACGGCT ATGGCAGGAT GGGCCTTCTC 
CACCAGGAGA AGCTCCCGAG TGACTTGGAT GGCATGTTCA TTGAGCGCTT GGACTGTGAC 
ATGGAGTCCA TCATCCGGAA CGACCTCATG GATGGAGACA CTTTGGATTT TAACTTTGAC 
AATGTGTTGC CCAACCAAAG CTTCCCACAC AGTGTCAAGA CGACGACACA TAGCTGGGTG 
TCAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FOXO1 ( XM_021065269.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020920928.1 Sus scrofa forkhead box O1 (FOXO1), mRNA.

Price & Availability↑

CloneID	OSe232551
Clone ID Related Accession (Same CDS sequence)	XM_021065269.1 , NM_214014.3
Accession Version	XM_021065269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	forkhead box protein O1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010453.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAAG CGCCCCAGGT GGTGGAGATC GACCCGGACT TCGAGCCGCT GCCCCGGCCG 
CGCTCGTGCA CCTGGCCGCT GCCCAGGCCG GAGTTTAGCC AGTCTAACTC GGCCACCTCC 
AGCCCGGCGC CGTCGGGTGG CCCAGCCGCG AACCCCGACG CTGCCGCGGG CCTGCCCTCG 
GCCTCGGCGG CCGCTGTCAA CGCCGACTTC ATGAGCAACC TGAGCCTGCT GGAGGAGAGC 
GGGGACTTCC AGCAGGCGCC CGGCTCCGTG GCGGCGGCGG CGGCGGCGGC AGCCGCGGTG 
GCGGCGGCGG CCGCCGCTGC TGCCACCGGG GGGTTGTGCG GGGACTTCCA GGGCCCAGAG 
GCGGGCTGCC TGCACCCGGC GCCGCCGCAG CAGCCCCCGC CGCCCGGGCC GCTGTCGCAG 
CACCCGCCTG TGCCCCCCGC CGCCGCCGGG TCGCTTGCCG GGCAGCCGCG CAAGAGCAGC 
TCGTCCCGCC GCAACGCGTG GGGCAACCTG TCCTACGCCG ACCTCATCAC CAAGGCCATC 
GAGAGCTCGG CAGAGAAGCG GCTCACGCTA TCGCAGATCT ACGAGTGGAT GGTCAAGAGC 
GTGCCCTACT TCAAGGATAA GGGCGACAGC AACAGCTCGG CAGGCTGGAA GAATTCAATT 
CGTCATAATC TGTCCCTACA CAGCAAGTTC ATTCGCGTGC AGAATGAAGG AACTGGAAAA 
AGTTCCTGGT GGATGCTCAA CCCAGAGGGA GGCAAGAGTG GGAAATCCCC CAGGAGAAGA 
GCCGCCTCCA TGGACAACAA CAGTAAATTT GCCAAGAGCC GAGGCCGGGC TGCCAAGAAA 
AAAGCATCCC TCCAGTCTGG CCAGGAGGGT GCCGGGGACA GCCCGGGATC TCAGTTTTCG 
AAGTGGCCCG CCAGCCCTGG CTCTCACAGC AACGATGACT TTGATAACTG GAGTACATTT 
CGGCCGCGCA CTAGCTCGAA TGCTAGTACT ATTAGTGGGA GGCTCTCTCC CATCATGACT 
GAGCAGGATG ACCTTGGAGA CGGGGATGTG CATTCTATGG TGTACCCACC CTCGGCGGCA 
AAGATGGCTT CTACTCTGCC CAGTCTGTCT GAGATAAGCA ATCCTGAGAA CATGGAGAAC 
CTTTTGGATA ATCTCAACCT TCTCTCATCA CCAACGTCAT TAACTGTTTC AACCCAGTCT 
TCACCAGGCA CCATCATGCA GCAGACGCCA TGCTACTCAT TTGCGCCACC AAACACCAGT 
CTGAATTCAC CCAGCCCCAA CTACCAAAAA TACACGTATG GCCAATCCAG CATGAGCCCT 
TTGCCCCAGA TGCCTATGCA AACACTTCAG GACAGCAAAT CGAGTTACGG AGGCATGGCC 
CAGTATAACT GTGCAGCGGG ACTCTTGAAG GAGTTACTTA CTTCCGACTC TCCTCCCCAT 
AATGACATTA TGACGCCAGT CGATCCGGGG GTGGCCCAAC CCAACAGCCG AGTCCTTGGC 
CAGAATGTGC TGATGGGCCC TAGTTCGGTC ATGCCAGCCT ACGGCGGCCA GGCCTCTCAT 
AACAAAATGA TGAATCCCAG CTCCCACAGC CACCCTGGAC ACGCTCAGTC CACGTCTGCC 
GTCAATGGGC GTGCCTTGCC CCACGCGGTG AACACCATGC CCCACGCCTC GGGGATGAAC 
CGCCTGGCCC AGGCGAAGAC CGCTTTACAA GTGCCTCTGC CCCACCCCAT GCAGATGAAC 
GCCCTGGGGG GCTACTCCCC CGCCAGCACC TGCAACGGCT ATGGCAGGAT GGGCCTTCTC 
CACCAGGAGA AGCTCCCGAG TGACTTGGAT GGCATGTTCA TTGAGCGCTT GGACTGTGAC 
ATGGAGTCCA TCATCCGGAA CGACCTCATG GATGGAGACA CTTTGGATTT TAACTTTGAC 
AATGTGTTGC CCAACCAAAG CTTCCCACAC AGTGTCAAGA CGACGACACA TAGCTGGGTG 
TCAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020920928.1
CDS	27..2015
Translation	MAEAPQVVEIDPDFEPLPRPRSCTWPLPRPEFSQSNSATSSPAPSGGPAANPDAAAGLPSASAAAVNADFMSNLSLLEESGDFQQAPGSVAAAAAAAAAVAAAAAAAATGGLCGDFQGPEAGCLHPAPPQQPPPPGPLSQHPPVPPAAAGSLAGQPRKSSSSRRNAWGNLSYADLITKAIESSAEKRLTLSQIYEWMVKSVPYFKDKGDSNSSAGWKNSIRHNLSLHSKFIRVQNEGTGKSSWWMLNPEGGKSGKSPRRRAASMDNNSKFAKSRGRAAKKKASLQSGQEGAGDSPGSQFSKWPASPGSHSNDDFDNWSTFRPRTSSNASTISGRLSPIMTEQDDLGDGDVHSMVYPPSAAKMASTLPSLSEISNPENMENLLDNLNLLSSPTSLTVSTQSSPGTIMQQTPCYSFAPPNTSLNSPSPNYQKYTYGQSSMSPLPQMPMQTLQDSKSSYGGMAQYNCAAGLLKELLTSDSPPHNDIMTPVDPGVAQPNSRVLGQNVLMGPSSVMPAYGGQASHNKMMNPSSHSHPGHAQSTSAVNGRALPHAVNTMPHASGMNRLAQAKTALQVPLPHPMQMNALGGYSPASTCNGYGRMGLLHQEKLPSDLDGMFIERLDCDMESIIRNDLMDGDTLDFNFDNVLPNQSFPHSVKTTTHSWVSG

Target ORF information:

RefSeq Version	XM_021065269.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa forkhead box O1 (FOXO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021065269.1

ORF Insert Sequence:

ATGGCCGAAG CGCCCCAGGT GGTGGAGATC GACCCGGACT TCGAGCCGCT GCCCCGGCCG 
CGCTCGTGCA CCTGGCCGCT GCCCAGGCCG GAGTTTAGCC AGTCTAACTC GGCCACCTCC 
AGCCCGGCGC CGTCGGGTGG CCCAGCCGCG AACCCCGACG CTGCCGCGGG CCTGCCCTCG 
GCCTCGGCGG CCGCTGTCAA CGCCGACTTC ATGAGCAACC TGAGCCTGCT GGAGGAGAGC 
GGGGACTTCC AGCAGGCGCC CGGCTCCGTG GCGGCGGCGG CGGCGGCGGC AGCCGCGGTG 
GCGGCGGCGG CCGCCGCTGC TGCCACCGGG GGGTTGTGCG GGGACTTCCA GGGCCCAGAG 
GCGGGCTGCC TGCACCCGGC GCCGCCGCAG CAGCCCCCGC CGCCCGGGCC GCTGTCGCAG 
CACCCGCCTG TGCCCCCCGC CGCCGCCGGG TCGCTTGCCG GGCAGCCGCG CAAGAGCAGC 
TCGTCCCGCC GCAACGCGTG GGGCAACCTG TCCTACGCCG ACCTCATCAC CAAGGCCATC 
GAGAGCTCGG CAGAGAAGCG GCTCACGCTA TCGCAGATCT ACGAGTGGAT GGTCAAGAGC 
GTGCCCTACT TCAAGGATAA GGGCGACAGC AACAGCTCGG CAGGCTGGAA GAATTCAATT 
CGTCATAATC TGTCCCTACA CAGCAAGTTC ATTCGCGTGC AGAATGAAGG AACTGGAAAA 
AGTTCCTGGT GGATGCTCAA CCCAGAGGGA GGCAAGAGTG GGAAATCCCC CAGGAGAAGA 
GCCGCCTCCA TGGACAACAA CAGTAAATTT GCCAAGAGCC GAGGCCGGGC TGCCAAGAAA 
AAAGCATCCC TCCAGTCTGG CCAGGAGGGT GCCGGGGACA GCCCGGGATC TCAGTTTTCG 
AAGTGGCCCG CCAGCCCTGG CTCTCACAGC AACGATGACT TTGATAACTG GAGTACATTT 
CGGCCGCGCA CTAGCTCGAA TGCTAGTACT ATTAGTGGGA GGCTCTCTCC CATCATGACT 
GAGCAGGATG ACCTTGGAGA CGGGGATGTG CATTCTATGG TGTACCCACC CTCGGCGGCA 
AAGATGGCTT CTACTCTGCC CAGTCTGTCT GAGATAAGCA ATCCTGAGAA CATGGAGAAC 
CTTTTGGATA ATCTCAACCT TCTCTCATCA CCAACGTCAT TAACTGTTTC AACCCAGTCT 
TCACCAGGCA CCATCATGCA GCAGACGCCA TGCTACTCAT TTGCGCCACC AAACACCAGT 
CTGAATTCAC CCAGCCCCAA CTACCAAAAA TACACGTATG GCCAATCCAG CATGAGCCCT 
TTGCCCCAGA TGCCTATGCA AACACTTCAG GACAGCAAAT CGAGTTACGG AGGCATGGCC 
CAGTATAACT GTGCAGCGGG ACTCTTGAAG GAGTTACTTA CTTCCGACTC TCCTCCCCAT 
AATGACATTA TGACGCCAGT CGATCCGGGG GTGGCCCAAC CCAACAGCCG AGTCCTTGGC 
CAGAATGTGC TGATGGGCCC TAGTTCGGTC ATGCCAGCCT ACGGCGGCCA GGCCTCTCAT 
AACAAAATGA TGAATCCCAG CTCCCACAGC CACCCTGGAC ACGCTCAGTC CACGTCTGCC 
GTCAATGGGC GTGCCTTGCC CCACGCGGTG AACACCATGC CCCACGCCTC GGGGATGAAC 
CGCCTGGCCC AGGCGAAGAC CGCTTTACAA GTGCCTCTGC CCCACCCCAT GCAGATGAAC 
GCCCTGGGGG GCTACTCCCC CGCCAGCACC TGCAACGGCT ATGGCAGGAT GGGCCTTCTC 
CACCAGGAGA AGCTCCCGAG TGACTTGGAT GGCATGTTCA TTGAGCGCTT GGACTGTGAC 
ATGGAGTCCA TCATCCGGAA CGACCTCATG GATGGAGACA CTTTGGATTT TAACTTTGAC 
AATGTGTTGC CCAACCAAAG CTTCCCACAC AGTGTCAAGA CGACGACACA TAGCTGGGTG 
TCAGGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PDGFR? Regulated by miR-34a and FoxO1 Promotes Adipogenesis in Porcine Intramuscular Preadipocytes through Erk Signaling Pathway.
International journal of molecular sciences18(11)(2017 Nov)
Sun YM,Qin J,Liu SG,Cai R,Chen XC,Wang XM,Pang WJ

Effects of MicroRNA-27a on Myogenin Expression and Akt/FoxO1 Signal Pathway during Porcine Myoblast Differentiation.
Animal biotechnology1-7(2017 Aug)
Zhang S,Chen X,Huang Z,Chen D,Yu B,He J,Zheng P,Yu J,Luo J,Luo Y,Chen H

FOXO1-suppressed miR-424 regulates the proliferation and osteogenic differentiation of MSCs by targeting FGF2 under oxidative stress.
Scientific reports742331(2017 Feb)
Li L,Qi Q,Luo J,Huang S,Ling Z,Gao M,Zhou Z,Stiehler M,Zou X

Glycine enhances muscle protein mass associated with maintaining Akt-mTOR-FOXO1 signaling and suppressing TLR4 and NOD2 signaling in piglets challenged with LPS.
American journal of physiology. Regulatory, integrative and comparative physiology311(2)R365-73(2016 Aug)
Liu Y,Wang X,Wu H,Chen S,Zhu H,Zhang J,Hou Y,Hu CA,Zhang G

Let-7g induces granulosa cell apoptosis by targeting MAP3K1 in the porcine ovary.
The international journal of biochemistry & cell biology68148-57(2015 Nov)
Cao R,Wu W,Zhou X,Liu K,Li B,Huang X,Zhang Y,Liu H

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FOXO1 cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

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