CEP250 gene cDNA ORF clone, Sus scrofa(pig)

mRNA	Protein	Name
XM_001929408.4	XP_001929443.1	centrosome-associated protein CEP250 isoform X1
XM_013985306.2	XP_013840760.1	centrosome-associated protein CEP250 isoform X1
XM_013985309.2	XP_013840763.1	centrosome-associated protein CEP250 isoform X3
XM_021078259.1	XP_020933918.1	centrosome-associated protein CEP250 isoform X6
XM_021078260.1	XP_020933919.1	centrosome-associated protein CEP250 isoform X7
XM_021078262.1	XP_020933921.1	centrosome-associated protein CEP250 isoform X9
XM_001929408.3	XP_001929443.1	centrosome-associated protein CEP250 isoform X1
XM_013985307.1	XP_013840761.1	centrosome-associated protein CEP250 isoform X1
XM_013985310.1	XP_013840764.1	centrosome-associated protein CEP250 isoform X3
XM_013985314.1	XP_013840768.1	centrosome-associated protein CEP250 isoform X6
XM_013985309.1	XP_013840763.1	centrosome-associated protein CEP250 isoform X2
XM_013985312.1	XP_013840766.1	centrosome-associated protein CEP250 isoform X4
XM_021078256.1	XP_020933915.1	centrosome-associated protein CEP250 isoform X2
XM_021078257.1	XP_020933916.1	centrosome-associated protein CEP250 isoform X4
XM_021078261.1	XP_020933920.1	centrosome-associated protein CEP250 isoform X8
XM_013985308.1	XP_013840762.1	centrosome-associated protein CEP250 isoform X1
XM_013985313.1	XP_013840767.1	centrosome-associated protein CEP250 isoform X5
XM_013985306.1	XP_013840760.1	centrosome-associated protein CEP250 isoform X1
XM_013985307.2	XP_013840761.1	centrosome-associated protein CEP250 isoform X1
XM_021078258.1	XP_020933917.1	centrosome-associated protein CEP250 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OSe241636	XM_021078262.1 Latest version!	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OSe241632	XM_021078256.1 Latest version!	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe241633	XM_021078258.1 Latest version!	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSe241634	XM_021078259.1 Latest version!	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSe241635	XM_021078261.1 Latest version!	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSe24918	XM_013985306.2 Latest version!	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe24918	XM_001929408.4 Latest version!	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe24918	XM_013985307.2 Latest version!	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe24918	XM_013985308.1 Latest version!	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe44857	XM_013985309.2 Latest version!	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe44858	XM_013985310.1 Latest version!	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe44859	XM_021078257.1 Latest version!	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe44859	XM_013985312.1 Latest version!	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe44860	XM_013985313.1 Latest version!	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSe44861	XM_021078260.1 Latest version!	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OSe44861	XM_013985314.1 Latest version!	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OSe24918	XM_001929408.3	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe44857	XM_013985309.1	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe24918	XM_013985306.1	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OSe24918	XM_013985307.1	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
Hide Old Accession Versions >>

CEP250 ( XM_021078262.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020933921.1 Sus scrofa centrosomal protein 250 (CEP250), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OSe241636
Clone ID Related Accession (Same CDS sequence)	XM_021078262.1
Accession Version	XM_021078262.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	centrosome-associated protein CEP250 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010459.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG CAGAGCAGGC AAGAAGCGCT TTGCAGGTGG GCCTGGCAGA GGTGGAGAGG 
AGCAGGGAAG CGCTGTGGGA AAAGAACACT TACCTGGAGG CTCAGCTGCA AAAAGCAGAG 
GCGGCCAGGG CTGAGCTGCA GGCAGATCTC AGGGGCGTCC AGGAAGAGAA GGAAGAAATT 
CAAGAGAAAC TAAGCGAGGC ACATCACCAG CAGGAGGCAG TCTCAGTTCA GATGGAGCAG 
CTGCGTCAGG AGGCAAAGCG GCAGGAAGAG GTGCTCGCCC GGGAAGTCCA GGAGAAGGAG 
GCTCTGGTGC GGGAAAAGGC GGCTCTTGAA GTGCGGCTGC AGGCTGTGGA GCGGGACCGG 
CAGGACCTCT CTGAACAACT GCTGGGGCTC AGCTCGGCCA AGGAGCTTCT GGAGAACAGT 
CTGTTTGAGG CCCAGCAACA GAATTCTCTG ATAGAAGTCA CCAAGGGGCA GCTGGAGGTC 
CAGATTCAAA CTATCACTCA AGCCAAGGAG GTAATCCAAG GGGAAGTGAG GTGCCTACAG 
CTGGAACTGG ATACTGAACG GAGCCGGGCA GAACAGGAGC GGGATGCTGC AACCAAACAG 
CTGGCCCAGG CTGAGCAAGA GGGGCAGACC GCCCTGCAGC AGCAGAAGTC AGCCCATGAG 
GAGGAAGTGA AGGGGCTCCA GGAAAAATGG GAGAAAGAGC GTGCCTGGCA CCAGCAGGAA 
CTGGATAAGG CTCTGAAGAG CCTTGAGAGG GAGAAAACGG AGCTGGAAAT GAGGCTGAGG 
GAGCAGCAGG CAGAAACAGA GGCCATGCGG ACGCAGAGGG AAGAAGAACG GGCTGAGGCT 
GAGAGCACCC TGCGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAATGTC CCTCCTGGAG 
ACACTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTGGA GCGGCTGAGG 
CAGGACATGA AGGTTCAGAA GTTAAAGGAG CAGGAGACCA CTGGGATGCT GCAGACCCAG 
CTCCGGGAAG CTCAGAAGGA GCTGGAACAG GCAGCCCAGC AGCACAGAGA CAGCCTTGCT 
GCCCTCCAAG AAGAAGGCCG TGCCCTGCTG CAGGATAAGA TAGACCTGCA CAAGCAGGTG 
GAGGACTTGA AGTCTCAGCT GGTTTCCAAG GAAGACTCCC AAAGGCTGGT AGACCAGGAA 
GTTCAGGAGA AGCTGAGAGA GGCCCAGGAA TGTAGCCGAA TTCAGAAGGA GCTGGAGAAA 
GAAAAGGCCA GCCTGACTCT GTTGCTGGTG GAAAAGGAAC AGAGACTCCT TGGTTTACAA 
GAGGCCGACT CCATTCGACA ACAAGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGGCC 
CAGGCAGGGC AGAAAGAGCT CAGCGCCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGCA 
AAGGAGACTG ACTTTCTGGC CCAGGAAGCG CGGCTGCTGG AAGAGCTGGA GGCATCTCAA 
GTAACAGAGC AGCAGCTACG AGCTTCCTTG TGGGCCCAGG AAGCCAAGGC CGCACAACTA 
CAGCTGCGAC TGCGCAGCAC AGAGAGCCAG CTGGAGGTGC TGCTGGCAGA GCAGCAGCCT 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTTTGTGCTG TCCTGCAGCA GGCCCTGGGG 
TCTGTTTGTG AGAGCAGGCC TGAGCTGAGT GGCGGGGGAG ACTCTGCTCC CTCCCTCTGG 
GGCCCTGAGC CAGACCAGAA TGGAGCTAGG ACCCTTTTCA AGAGAGGGCC CCTCCTGACA 
GCTCTCTCGG CTGAGGCGGT GGCCTCTGCC CTTCACAAGC TTAACCAAGA CCTGTGGAAG 
ACTCAGCAGG CCCGGGATGA TCTGAGGGAT CAGGCCCAGA AGCTGGGACA ACGTCTTACT 
GAAACAGAGG CCGAGAAGAG CCAGCTCCAC ACAGAGTTGC AGGCTCTGCA GAGGCAGCTC 
TCCCAGAACC AGGAAGAGAG ATCCAAGTGG GAAGGAAAAC AGACCTCCTT AGAATCTGAG 
CTGGTGGAAC TGCATGAAAC TGTGACATCC TTACAGAGTC GCCTACGGCA AGCAGAGCTG 
CAGGGAATAG AAGCCCAGAA TGAGCAAGAG TTACTGCAGG CAGCCAAGGA GAACCTGACA 
GCCCAGGTGG AACACCTCCA AGCAGCTGTT GCAGAAGCCA GGGCTCAGGC AAGCGCTGCC 
GAGGTCCTGG AGGAAGACCT CAAAACTGCA CGCTCAGCCC TGAAACTGAA AAGCAAGGAG 
GTGGAGAGTG AGCGTGAGCG AGCCCAGGCC CTACAGGAGC AGGGCGAGCT GAAGGTGGCC 
CAAGGGAAAG CTTTGCAGGA GAATTTGGCT CGCCTGACTC AGACCCTATC TGAGAGAGAA 
GGGGAGGTGG AAACTTTGCA GGGAAAAATC CAGGAGCTGG AGAAGCAACG GGAAATGCAA 
AAGGCTGCTT TGGAGGTGCT GTCTTTGGAC CTCAAAAAGA GGAACCAAGA GGTGGATCTG 
CAGCAAGAAC AGATTCAGGA GCTGGAGGAG TGCAGATCGG TTTTAGAGCA TCTCCCCATG 
GCAGTCCAGG AGCGAGAGCA GAAGCTGACT CTGCAGAGGG AGCAGATCAA GGAGCTCGAG 
AAGGACCGAG AGACACAGAG GAACATCTTG GAGCATCAGC TTCTGGAACT TGAGAAGAAG 
GCCCAAGTGA TTGAGTCCCA GAAAGGACAG ATTCAGGATC TGAAGAAGCA GCTGGTTACT 
CTGGAATGCC TGGCCCTGGA ACTAGAGGAA AACCATCACA AAATAGAGTG CCAGCAAAAG 
ACAATTGAGG AGCTGGAGGG CCAGAGGGAA ATGCAGAGGG CAGCTCTGAC CCATCTGACA 
CTGGACCTGG AGGAAAGGAG CCAGGAGCTG CAGGTACAGG GCAGCCAGAT CCGGGAGCTA 
GAGAGTCACA ACAGCCTCTT GGCAAGAGAG CTCCAGGAGA AGGACCAGGA GATGACGTCC 
CAGCAAGACC AGACAGAGGA ACTGCAGAGG CAGAAGGAGC ATTTGACACA GGACCTCAAG 
AGGCGGGAAC AGGAGATAGT GCTGCTGAAG GAGAGGATTC AGGTCTTGGA AGATCAGAGA 
ACCCTACAGA CCAAGATCCT AGAGGAGGAC CTGGAGCAGA TCAAAATGTC CTTGAGGGAG 
CGAGGCCGGG ACCTGGCCTC GCAGAGGCAG CTGATGCAGG AGCGGGCAGA GGAAGGGAAG 
GGCCAGAGTA AAGCACAGCG CGGGAGCCTG GAGCATATGA AGCTGATCCT GCGTGATAAG 
GAGAAGGAGG TGGAATGCCA GCAGGAGCAT ATCCAGGAAC TGCAGGAGCA CAAGGCCCAG 
CTGGAGCAGC AGCTCCAGGG CTTACACAGG AAGATGGGGG AGACCAGCCT TCTCCTGACC 
CAGCGGGAGC AGGAGATAGT GGCTCTGCAG CGGCACCTGC AGGAAGCCAG GGAACAGGGA 
GAGCTGAAAG AGCAGTCACT TCAAGGTCAG CTGGAAGAGG CCCAGAGGGC GCTGGCCCGA 
AGGGACCAGG AGCTCAAGGC CCTGCAGCAG CAGCAGGCCC AAGGCCAGGA GGAAAATGTG 
ATGGAAAAGG CGGGCTTACT GCAGAGAGAG CTGGAGCAGG CCCACGTGAC ACTGAAGGAG 
CGCCAGGCGG AGCTTGAGGA TTACAAGGAG CATGTGAGAA GGCTCCAGGA AGAGCTGGCA 
GTGGAGGGGC AGCGGGTACA GGCCCTGGAG GAGGTGTTGA GTGACCTAAG GGCTGAATCT 
CGGGAACAAG AGAAGGCCCT GCTGGCCCTC CAGCAGCAGT GTGCCGAGCA GGCCCAGGAG 
CACGAGGCAG AGGCCAGGGC CCTGCAGGAC AGCTGGCTGC AGGCAGAGGC TATGCTCAAG 
GAACGGGACC AGGAACTGGA GGCCCTGCGG GCAGAGGGCC TGTCTTCCCA GCATCGGGAG 
GAGGCTGCCT GTGGCCAGGT GGAGGCTCTG CAGGAGGCCC TCAGCAAGGC TCAGGCTGAC 
CTGCAGGAGA AAGAGCAGCA TCTCCTTGGG CAGGTGGAAC TGAGCCGCAG CCTAGAGGCC 
AGCACGGCCA CGTTGCAGGC TGCCCTGGAC TCCTGCCAGG CACAGTCCCG GCAGCTGGAG 
GAGGCCCTGA GGAAGCGGGA AGGTGAGGTC CAGGTCCGGG ACCTCCGATA CCAGGAGGCC 
ATGCAGCAGC TCCAGCAGGC ACTTACCCAG AGGGATGAAG AGCTGAGACA ACAGAGGAAA 
CAGGGGCAGC TCCTGGAGAA GTCTCTGGCC CAGAGGGGCC AAGAGGATGC AACCCAAGAG 
AAGCAAGAAG CAAAGCACGA GAGAGAAGAG GAAGAGATAA GGAGCCTTCG TGAGAGTCTG 
CAGGAGCTAC AGCTGACTCT AGCACAAAAG GAAGAGGAGA TCCTGGAGCT GAGGGAGGCC 
CAGCAGAGGA AGAGTCTGGA GGACTTGTCT CACAAAGGCT GCCCAGGGAA GGAGCCGGCT 
ACAACATTTG ATTCCTTAGG GCCCAGGCTG CAGCGGGAAC TGGAGCGACT ACAGGCAGCA 
CTGAAGCAGA CAGAAGCCAG GGAGATCGAG TGGAGGGAGA AGGCCCAGGA TCTGGCGCTG 
TCCCTGGCCC AGAGCAAGGC CAGTGTCAGC GATCTGCAGG AGGTAGCCCT GTTCCTGCAA 
GCATCTGTTC TGGAGCGGGA CTCAGAACAG CAGAGGCTGC AGGATGAGTT GGAGCTCACC 
AAACTCACTC TGGAGAAAGA GCGACTCCAC AGCCCAGGCC CGAGCAGCAG AGCAGAACGG 
GGGCCTAGAG GAGAGCCAGG TGTACAACTG GGAGAGGTCT CGGGAGTGAA GACTGAGCCT 
AGTCCTGAGA TGGAGGAGAG GCAGCTCTGG GAACAGAGGC TTGAGCACCT GCAGCGAGCG 
GTGGCTCTCT TGGAGGTTGA CCGAAGCCAG CTGCAACAAC ATAACGCCCA GCTGCGGACC 
ACCCTGGAGC AGGTGGAGCG AGAGCGGAGG AAACTGAAGA GGGGTTCCAT GCGTGCATTG 
CGGGCAGGGG ACCTGGAGAG CGGTGAAGCC ACGGCACCAT CACCCATGCA ACAGGATGGA 
AGAGGAAGGC AGAAGGGCTG CTCAGATGCC AAGCACATGG TTGAGCTGCA AAAAGAGGTG 
GCCCTCCTGC GAGCCCAGCT GGCCTTGGAG CGGAAGCAGA AGCAGGACTA CATTGCTCGC 
TCAGTTCAGA CGAGCCGTGA GCTGGCGGAC CTGCACCACG GCCTCTCTCA CTCCCTTCTC 
GCTGTAGCCC AAGCCCCCGA GGCCACCGTC TTAGAGGCCG AGACCCGCAA GCTGGACGAG 
TCTCTGACTC AAAGCCTGAC ATCCCCCGGG CCAGCACTGC TGTGCCCCAG CTCCAGTGCT 
GCTCAGACTG CCTCCAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020933921.1
CDS	176..5596
Translation	MEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_021078262.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078262.1

ORF Insert Sequence:

ATGGAGGCAG CAGAGCAGGC AAGAAGCGCT TTGCAGGTGG GCCTGGCAGA GGTGGAGAGG 
AGCAGGGAAG CGCTGTGGGA AAAGAACACT TACCTGGAGG CTCAGCTGCA AAAAGCAGAG 
GCGGCCAGGG CTGAGCTGCA GGCAGATCTC AGGGGCGTCC AGGAAGAGAA GGAAGAAATT 
CAAGAGAAAC TAAGCGAGGC ACATCACCAG CAGGAGGCAG TCTCAGTTCA GATGGAGCAG 
CTGCGTCAGG AGGCAAAGCG GCAGGAAGAG GTGCTCGCCC GGGAAGTCCA GGAGAAGGAG 
GCTCTGGTGC GGGAAAAGGC GGCTCTTGAA GTGCGGCTGC AGGCTGTGGA GCGGGACCGG 
CAGGACCTCT CTGAACAACT GCTGGGGCTC AGCTCGGCCA AGGAGCTTCT GGAGAACAGT 
CTGTTTGAGG CCCAGCAACA GAATTCTCTG ATAGAAGTCA CCAAGGGGCA GCTGGAGGTC 
CAGATTCAAA CTATCACTCA AGCCAAGGAG GTAATCCAAG GGGAAGTGAG GTGCCTACAG 
CTGGAACTGG ATACTGAACG GAGCCGGGCA GAACAGGAGC GGGATGCTGC AACCAAACAG 
CTGGCCCAGG CTGAGCAAGA GGGGCAGACC GCCCTGCAGC AGCAGAAGTC AGCCCATGAG 
GAGGAAGTGA AGGGGCTCCA GGAAAAATGG GAGAAAGAGC GTGCCTGGCA CCAGCAGGAA 
CTGGATAAGG CTCTGAAGAG CCTTGAGAGG GAGAAAACGG AGCTGGAAAT GAGGCTGAGG 
GAGCAGCAGG CAGAAACAGA GGCCATGCGG ACGCAGAGGG AAGAAGAACG GGCTGAGGCT 
GAGAGCACCC TGCGCCAGAT GCAGCTCGAA ACAGAGAAGG AGAGAATGTC CCTCCTGGAG 
ACACTGCTGC AGACCCAGAA GGAGCTGGCA GATGCCAGCC AACAACTGGA GCGGCTGAGG 
CAGGACATGA AGGTTCAGAA GTTAAAGGAG CAGGAGACCA CTGGGATGCT GCAGACCCAG 
CTCCGGGAAG CTCAGAAGGA GCTGGAACAG GCAGCCCAGC AGCACAGAGA CAGCCTTGCT 
GCCCTCCAAG AAGAAGGCCG TGCCCTGCTG CAGGATAAGA TAGACCTGCA CAAGCAGGTG 
GAGGACTTGA AGTCTCAGCT GGTTTCCAAG GAAGACTCCC AAAGGCTGGT AGACCAGGAA 
GTTCAGGAGA AGCTGAGAGA GGCCCAGGAA TGTAGCCGAA TTCAGAAGGA GCTGGAGAAA 
GAAAAGGCCA GCCTGACTCT GTTGCTGGTG GAAAAGGAAC AGAGACTCCT TGGTTTACAA 
GAGGCCGACT CCATTCGACA ACAAGAGCTG AGCTCCCTGC GCCAGGACAT GCAGGAGGCC 
CAGGCAGGGC AGAAAGAGCT CAGCGCCCAG GTGGAATTAC TGAAGCAGGA GGTGAAGGCA 
AAGGAGACTG ACTTTCTGGC CCAGGAAGCG CGGCTGCTGG AAGAGCTGGA GGCATCTCAA 
GTAACAGAGC AGCAGCTACG AGCTTCCTTG TGGGCCCAGG AAGCCAAGGC CGCACAACTA 
CAGCTGCGAC TGCGCAGCAC AGAGAGCCAG CTGGAGGTGC TGCTGGCAGA GCAGCAGCCT 
GGGCACCAGG CCCAGGCCCA GCTGGCCAGC CTTTGTGCTG TCCTGCAGCA GGCCCTGGGG 
TCTGTTTGTG AGAGCAGGCC TGAGCTGAGT GGCGGGGGAG ACTCTGCTCC CTCCCTCTGG 
GGCCCTGAGC CAGACCAGAA TGGAGCTAGG ACCCTTTTCA AGAGAGGGCC CCTCCTGACA 
GCTCTCTCGG CTGAGGCGGT GGCCTCTGCC CTTCACAAGC TTAACCAAGA CCTGTGGAAG 
ACTCAGCAGG CCCGGGATGA TCTGAGGGAT CAGGCCCAGA AGCTGGGACA ACGTCTTACT 
GAAACAGAGG CCGAGAAGAG CCAGCTCCAC ACAGAGTTGC AGGCTCTGCA GAGGCAGCTC 
TCCCAGAACC AGGAAGAGAG ATCCAAGTGG GAAGGAAAAC AGACCTCCTT AGAATCTGAG 
CTGGTGGAAC TGCATGAAAC TGTGACATCC TTACAGAGTC GCCTACGGCA AGCAGAGCTG 
CAGGGAATAG AAGCCCAGAA TGAGCAAGAG TTACTGCAGG CAGCCAAGGA GAACCTGACA 
GCCCAGGTGG AACACCTCCA AGCAGCTGTT GCAGAAGCCA GGGCTCAGGC AAGCGCTGCC 
GAGGTCCTGG AGGAAGACCT CAAAACTGCA CGCTCAGCCC TGAAACTGAA AAGCAAGGAG 
GTGGAGAGTG AGCGTGAGCG AGCCCAGGCC CTACAGGAGC AGGGCGAGCT GAAGGTGGCC 
CAAGGGAAAG CTTTGCAGGA GAATTTGGCT CGCCTGACTC AGACCCTATC TGAGAGAGAA 
GGGGAGGTGG AAACTTTGCA GGGAAAAATC CAGGAGCTGG AGAAGCAACG GGAAATGCAA 
AAGGCTGCTT TGGAGGTGCT GTCTTTGGAC CTCAAAAAGA GGAACCAAGA GGTGGATCTG 
CAGCAAGAAC AGATTCAGGA GCTGGAGGAG TGCAGATCGG TTTTAGAGCA TCTCCCCATG 
GCAGTCCAGG AGCGAGAGCA GAAGCTGACT CTGCAGAGGG AGCAGATCAA GGAGCTCGAG 
AAGGACCGAG AGACACAGAG GAACATCTTG GAGCATCAGC TTCTGGAACT TGAGAAGAAG 
GCCCAAGTGA TTGAGTCCCA GAAAGGACAG ATTCAGGATC TGAAGAAGCA GCTGGTTACT 
CTGGAATGCC TGGCCCTGGA ACTAGAGGAA AACCATCACA AAATAGAGTG CCAGCAAAAG 
ACAATTGAGG AGCTGGAGGG CCAGAGGGAA ATGCAGAGGG CAGCTCTGAC CCATCTGACA 
CTGGACCTGG AGGAAAGGAG CCAGGAGCTG CAGGTACAGG GCAGCCAGAT CCGGGAGCTA 
GAGAGTCACA ACAGCCTCTT GGCAAGAGAG CTCCAGGAGA AGGACCAGGA GATGACGTCC 
CAGCAAGACC AGACAGAGGA ACTGCAGAGG CAGAAGGAGC ATTTGACACA GGACCTCAAG 
AGGCGGGAAC AGGAGATAGT GCTGCTGAAG GAGAGGATTC AGGTCTTGGA AGATCAGAGA 
ACCCTACAGA CCAAGATCCT AGAGGAGGAC CTGGAGCAGA TCAAAATGTC CTTGAGGGAG 
CGAGGCCGGG ACCTGGCCTC GCAGAGGCAG CTGATGCAGG AGCGGGCAGA GGAAGGGAAG 
GGCCAGAGTA AAGCACAGCG CGGGAGCCTG GAGCATATGA AGCTGATCCT GCGTGATAAG 
GAGAAGGAGG TGGAATGCCA GCAGGAGCAT ATCCAGGAAC TGCAGGAGCA CAAGGCCCAG 
CTGGAGCAGC AGCTCCAGGG CTTACACAGG AAGATGGGGG AGACCAGCCT TCTCCTGACC 
CAGCGGGAGC AGGAGATAGT GGCTCTGCAG CGGCACCTGC AGGAAGCCAG GGAACAGGGA 
GAGCTGAAAG AGCAGTCACT TCAAGGTCAG CTGGAAGAGG CCCAGAGGGC GCTGGCCCGA 
AGGGACCAGG AGCTCAAGGC CCTGCAGCAG CAGCAGGCCC AAGGCCAGGA GGAAAATGTG 
ATGGAAAAGG CGGGCTTACT GCAGAGAGAG CTGGAGCAGG CCCACGTGAC ACTGAAGGAG 
CGCCAGGCGG AGCTTGAGGA TTACAAGGAG CATGTGAGAA GGCTCCAGGA AGAGCTGGCA 
GTGGAGGGGC AGCGGGTACA GGCCCTGGAG GAGGTGTTGA GTGACCTAAG GGCTGAATCT 
CGGGAACAAG AGAAGGCCCT GCTGGCCCTC CAGCAGCAGT GTGCCGAGCA GGCCCAGGAG 
CACGAGGCAG AGGCCAGGGC CCTGCAGGAC AGCTGGCTGC AGGCAGAGGC TATGCTCAAG 
GAACGGGACC AGGAACTGGA GGCCCTGCGG GCAGAGGGCC TGTCTTCCCA GCATCGGGAG 
GAGGCTGCCT GTGGCCAGGT GGAGGCTCTG CAGGAGGCCC TCAGCAAGGC TCAGGCTGAC 
CTGCAGGAGA AAGAGCAGCA TCTCCTTGGG CAGGTGGAAC TGAGCCGCAG CCTAGAGGCC 
AGCACGGCCA CGTTGCAGGC TGCCCTGGAC TCCTGCCAGG CACAGTCCCG GCAGCTGGAG 
GAGGCCCTGA GGAAGCGGGA AGGTGAGGTC CAGGTCCGGG ACCTCCGATA CCAGGAGGCC 
ATGCAGCAGC TCCAGCAGGC ACTTACCCAG AGGGATGAAG AGCTGAGACA ACAGAGGAAA 
CAGGGGCAGC TCCTGGAGAA GTCTCTGGCC CAGAGGGGCC AAGAGGATGC AACCCAAGAG 
AAGCAAGAAG CAAAGCACGA GAGAGAAGAG GAAGAGATAA GGAGCCTTCG TGAGAGTCTG 
CAGGAGCTAC AGCTGACTCT AGCACAAAAG GAAGAGGAGA TCCTGGAGCT GAGGGAGGCC 
CAGCAGAGGA AGAGTCTGGA GGACTTGTCT CACAAAGGCT GCCCAGGGAA GGAGCCGGCT 
ACAACATTTG ATTCCTTAGG GCCCAGGCTG CAGCGGGAAC TGGAGCGACT ACAGGCAGCA 
CTGAAGCAGA CAGAAGCCAG GGAGATCGAG TGGAGGGAGA AGGCCCAGGA TCTGGCGCTG 
TCCCTGGCCC AGAGCAAGGC CAGTGTCAGC GATCTGCAGG AGGTAGCCCT GTTCCTGCAA 
GCATCTGTTC TGGAGCGGGA CTCAGAACAG CAGAGGCTGC AGGATGAGTT GGAGCTCACC 
AAACTCACTC TGGAGAAAGA GCGACTCCAC AGCCCAGGCC CGAGCAGCAG AGCAGAACGG 
GGGCCTAGAG GAGAGCCAGG TGTACAACTG GGAGAGGTCT CGGGAGTGAA GACTGAGCCT 
AGTCCTGAGA TGGAGGAGAG GCAGCTCTGG GAACAGAGGC TTGAGCACCT GCAGCGAGCG 
GTGGCTCTCT TGGAGGTTGA CCGAAGCCAG CTGCAACAAC ATAACGCCCA GCTGCGGACC 
ACCCTGGAGC AGGTGGAGCG AGAGCGGAGG AAACTGAAGA GGGGTTCCAT GCGTGCATTG 
CGGGCAGGGG ACCTGGAGAG CGGTGAAGCC ACGGCACCAT CACCCATGCA ACAGGATGGA 
AGAGGAAGGC AGAAGGGCTG CTCAGATGCC AAGCACATGG TTGAGCTGCA AAAAGAGGTG 
GCCCTCCTGC GAGCCCAGCT GGCCTTGGAG CGGAAGCAGA AGCAGGACTA CATTGCTCGC 
TCAGTTCAGA CGAGCCGTGA GCTGGCGGAC CTGCACCACG GCCTCTCTCA CTCCCTTCTC 
GCTGTAGCCC AAGCCCCCGA GGCCACCGTC TTAGAGGCCG AGACCCGCAA GCTGGACGAG 
TCTCTGACTC AAAGCCTGAC ATCCCCCGGG CCAGCACTGC TGTGCCCCAG CTCCAGTGCT 
GCTCAGACTG CCTCCAGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_021078256.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020933915.1 Sus scrofa centrosomal protein 250 (CEP250), transcript variant X4, mRNA.

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CloneID	OSe241632
Clone ID Related Accession (Same CDS sequence)	XM_021078256.1
Accession Version	XM_021078256.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	centrosome-associated protein CEP250 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010459.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATT GTGAAGAGGC CTGCACAAAC 
CTTGTTCCAA TAGGAATGGC ATATGTCTTC CACAGGATGG AAGAGGAAGG CAGAAGGGCT 
GCTCAGATGC CAAGCACATG GTTGAGCTGC AAAAAGAGGT GGCCCTCCTG CGAGCCCAGC 
TGGCCTTGGA GCGGAAGCAG AAGCAGGACT ACATTGCTCG CTCAGTTCAG ACGAGCCGTG 
AGCTGGCGGA CCTGCACCAC GGCCTCTCTC ACTCCCTTCT CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020933915.1
CDS	1175..8422
Translation	METGRPRLNNMKPQPLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQATLVRKLQAKVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDCEEACTNLVPIGMAYVFHRMEEEGRRAAQMPSTWLSCKKRWPSCEPSWPWSGSRSRTTLLAQFRRAVSWRTCTTASLTPFSL

Target ORF information:

RefSeq Version	XM_021078256.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078256.1

ORF Insert Sequence:

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATT GTGAAGAGGC CTGCACAAAC 
CTTGTTCCAA TAGGAATGGC ATATGTCTTC CACAGGATGG AAGAGGAAGG CAGAAGGGCT 
GCTCAGATGC CAAGCACATG GTTGAGCTGC AAAAAGAGGT GGCCCTCCTG CGAGCCCAGC 
TGGCCTTGGA GCGGAAGCAG AAGCAGGACT ACATTGCTCG CTCAGTTCAG ACGAGCCGTG 
AGCTGGCGGA CCTGCACCAC GGCCTCTCTC ACTCCCTTCT CGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_021078258.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020933917.1 Sus scrofa centrosomal protein 250 (CEP250), transcript variant X7, mRNA.

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CloneID	OSe241633
Clone ID Related Accession (Same CDS sequence)	XM_021078258.1
Accession Version	XM_021078258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	centrosome-associated protein CEP250 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010459.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAG CTGACGTGGT GAATAAAGCG CTTCGGGAAG ATGTGGAAAA ATTGACAGTA 
GACTGGAGCC AGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CATGGTCGCC TTCTCAGTCT ATGGCGGGAG 
GTGGTGACCT TCCGACGTCA CTTTCTGGAA ATGAAGTCAG CCACTGACAG AGACCTGATG 
GAGCTCAAGG CTGAGCACGT GAAGCTCTCT GGGTCCCTGT TGACCTGTTG TCTGCGCTTG 
ACTGTGGGAG CACCACAGGC CCGGGAATCC ACTGGATCTG GGAGGCTGAA TGGGAGTGAA 
CCATCCCAGC TGATGCTGCT ACTGTCTAAG ACCCAGGAGC TGGAGAAGGA AGCCCATAAA 
AGGAGCCAGG AGTTAATACA GCTTAAGAGT CAAGGGGATC TGGAGAAGGC TGAGCTTCAG 
GATCGGGTGA CCGAGCTTTC TGCTCTGCTG ACCCAGTCTC AGAAGCAGAA TGAAGATTAT 
GAAAAGATGG TAAAGGCTCT GAGAGAGACA ATGAAGATCC TGGAGGCAAA TCATGCAGAG 
TTAATGGAAC ATGAGGCATC TCTTAGTAGG AATGCCCAAG AGGAGAGGCT GTCTTTACAG 
CAAGTGATCA AGGATATAAC CCAGGCCATG GTAGAAGAAG GGGACCATAT GGCCCAAGGC 
TCTGGTCACG AGCACTCCTT GGACTTGGAT CCTAGTGGCT TCTTCTCCCA GTTTGATTCC 
CAGCAGCCAG ACAAGGCCCT CACTCTGGTG CGTTCTGTGT TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAAGCAGCA GCTTTCAGGC TGTCAGGAAG CTGTGAGCTC CTTGCAGCAG 
CAGCATCATC AGTGGGAAGA AGAGGGTGAG GCCTTGAGAC AGCGGCTGCA GAAGCTCACC 
GGGGAGCGGG ACACCCTGGC AGGGCAGACT GTGGGCCTGC AGGGGGAGGT GGACTCTCTG 
AGCAGGGAGC GAGAGCTCCT GCAAAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCCTGGCG ACTACGGAGG ACAAACATGG AGCTTCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAGCAGCAG GAGGAGCTGC ACCTGGCTGT CCGAGAACGG 
GAGCGCCTTC AGGAGATGCT GGCGAGCCTG GAAGCCAAAC AGTCAGAATC TCTCAGTGAA 
CTGATCACCC TTCGGGAAGC CCTGGAGTCT AGTCGCCTAG AAGGGGAGTT GCTGAGGCAA 
GAGCAAACAG AAGTGACTGC TGTGCTAGCA AAGGCAGAAC AGTCTATTGT GGAGCTGTCA 
AGTTCTGAGA ACAGCCTGAA GGCTGAGGTA GCTGATCTTC GGGCTGCAGC TGTCAAGCTC 
AGTGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGC AGAACCAGTC TGTGTGCAGC AGGATGGAGG CAGCAGAGCA GGCAAGAAGC 
GCTTTGCAGG TGGGCCTGGC AGAGGTGGAG AGGAGCAGGG AAGCGCTGTG GGAAAAGAAC 
ACTTACCTGG AGGCTCAGCT GCAAAAAGCA GAGGCGGCCA GGGCTGAGCT GCAGGCAGAT 
CTCAGGGGCG TCCAGGAAGA GAAGGAAGAA ATTCAAGAGA AACTAAGCGA GGCACATCAC 
CAGCAGGAGG CAGTCTCAGT TCAGATGGAG CAGCTGCGTC AGGAGGCAAA GCGGCAGGAA 
GAGGTGCTCG CCCGGGAAGT CCAGGAGAAG GAGGCTCTGG TGCGGGAAAA GGCGGCTCTT 
GAAGTGCGGC TGCAGGCTGT GGAGCGGGAC CGGCAGGACC TCTCTGAACA ACTGCTGGGG 
CTCAGCTCGG CCAAGGAGCT TCTGGAGAAC AGTCTGTTTG AGGCCCAGCA ACAGAATTCT 
CTGATAGAAG TCACCAAGGG GCAGCTGGAG GTCCAGATTC AAACTATCAC TCAAGCCAAG 
GAGGTAATCC AAGGGGAAGT GAGGTGCCTA CAGCTGGAAC TGGATACTGA ACGGAGCCGG 
GCAGAACAGG AGCGGGATGC TGCAACCAAA CAGCTGGCCC AGGCTGAGCA AGAGGGGCAG 
ACCGCCCTGC AGCAGCAGAA GTCAGCCCAT GAGGAGGAAG TGAAGGGGCT CCAGGAAAAA 
TGGGAGAAAG AGCGTGCCTG GCACCAGCAG GAACTGGATA AGGCTCTGAA GAGCCTTGAG 
AGGGAGAAAA CGGAGCTGGA AATGAGGCTG AGGGAGCAGC AGGCAGAAAC AGAGGCCATG 
CGGACGCAGA GGGAAGAAGA ACGGGCTGAG GCTGAGAGCA CCCTGCGCCA GATGCAGCTC 
GAAACAGAGA AGGAGAGAAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGATGCCA GCCAACAACT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GAAGTTAAAG 
GAGCAGGAGA CCACTGGGAT GCTGCAGACC CAGCTCCGGG AAGCTCAGAA GGAGCTGGAA 
CAGGCAGCCC AGCAGCACAG AGACAGCCTT GCTGCCCTCC AAGAAGAAGG CCGTGCCCTG 
CTGCAGGATA AGATAGACCT GCACAAGCAG GTGGAGGACT TGAAGTCTCA GCTGGTTTCC 
AAGGAAGACT CCCAAAGGCT GGTAGACCAG GAAGTTCAGG AGAAGCTGAG AGAGGCCCAG 
GAATGTAGCC GAATTCAGAA GGAGCTGGAG AAAGAAAAGG CCAGCCTGAC TCTGTTGCTG 
GTGGAAAAGG AACAGAGACT CCTTGGTTTA CAAGAGGCCG ACTCCATTCG ACAACAAGAG 
CTGAGCTCCC TGCGCCAGGA CATGCAGGAG GCCCAGGCAG GGCAGAAAGA GCTCAGCGCC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GCAAAGGAGA CTGACTTTCT GGCCCAGGAA 
GCGCGGCTGC TGGAAGAGCT GGAGGCATCT CAAGTAACAG AGCAGCAGCT ACGAGCTTCC 
TTGTGGGCCC AGGAAGCCAA GGCCGCACAA CTACAGCTGC GACTGCGCAG CACAGAGAGC 
CAGCTGGAGG TGCTGCTGGC AGAGCAGCAG CCTGGGCACC AGGCCCAGGC CCAGCTGGCC 
AGCCTTTGTG CTGTCCTGCA GCAGGCCCTG GGGTCTGTTT GTGAGAGCAG GCCTGAGCTG 
AGTGGCGGGG GAGACTCTGC TCCCTCCCTC TGGGGCCCTG AGCCAGACCA GAATGGAGCT 
AGGACCCTTT TCAAGAGAGG GCCCCTCCTG ACAGCTCTCT CGGCTGAGGC GGTGGCCTCT 
GCCCTTCACA AGCTTAACCA AGACCTGTGG AAGACTCAGC AGGCCCGGGA TGATCTGAGG 
GATCAGGCCC AGAAGCTGGG ACAACGTCTT ACTGAAACAG AGGCCGAGAA GAGCCAGCTC 
CACACAGAGT TGCAGGCTCT GCAGAGGCAG CTCTCCCAGA ACCAGGAAGA GAGATCCAAG 
TGGGAAGGAA AACAGACCTC CTTAGAATCT GAGCTGGTGG AACTGCATGA AACTGTGACA 
TCCTTACAGA GTCGCCTACG GCAAGCAGAG CTGCAGGGAA TAGAAGCCCA GAATGAGCAA 
GAGTTACTGC AGGCAGCCAA GGAGAACCTG ACAGCCCAGG TGGAACACCT CCAAGCAGCT 
GTTGCAGAAG CCAGGGCTCA GGCAAGCGCT GCCGAGGTCC TGGAGGAAGA CCTCAAAACT 
GCACGCTCAG CCCTGAAACT GAAAAGCAAG GAGGTGGAGA GTGAGCGTGA GCGAGCCCAG 
GCCCTACAGG AGCAGGGCGA GCTGAAGGTG GCCCAAGGGA AAGCTTTGCA GGAGAATTTG 
GCTCGCCTGA CTCAGACCCT ATCTGAGAGA GAAGGGGAGG TGGAAACTTT GCAGGGAAAA 
ATCCAGGAGC TGGAGAAGCA ACGGGAAATG CAAAAGGCTG CTTTGGAGGT GCTGTCTTTG 
GACCTCAAAA AGAGGAACCA AGAGGTGGAT CTGCAGCAAG AACAGATTCA GGAGCTGGAG 
GAGTGCAGAT CGGTTTTAGA GCATCTCCCC ATGGCAGTCC AGGAGCGAGA GCAGAAGCTG 
ACTCTGCAGA GGGAGCAGAT CAAGGAGCTC GAGAAGGACC GAGAGACACA GAGGAACATC 
TTGGAGCATC AGCTTCTGGA ACTTGAGAAG AAGGCCCAAG TGATTGAGTC CCAGAAAGGA 
CAGATTCAGG ATCTGAAGAA GCAGCTGGTT ACTCTGGAAT GCCTGGCCCT GGAACTAGAG 
GAAAACCATC ACAAAATAGA GTGCCAGCAA AAGACAATTG AGGAGCTGGA GGGCCAGAGG 
GAAATGCAGA GGGCAGCTCT GACCCATCTG ACACTGGACC TGGAGGAAAG GAGCCAGGAG 
CTGCAGGTAC AGGGCAGCCA GATCCGGGAG CTAGAGAGTC ACAACAGCCT CTTGGCAAGA 
GAGCTCCAGG AGAAGGACCA GGAGATGACG TCCCAGCAAG ACCAGACAGA GGAACTGCAG 
AGGCAGAAGG AGCATTTGAC ACAGGACCTC AAGAGGCGGG AACAGGAGAT AGTGCTGCTG 
AAGGAGAGGA TTCAGGTCTT GGAAGATCAG AGAACCCTAC AGACCAAGAT CCTAGAGGAG 
GACCTGGAGC AGATCAAAAT GTCCTTGAGG GAGCGAGGCC GGGACCTGGC CTCGCAGAGG 
CAGCTGATGC AGGAGCGGGC AGAGGAAGGG AAGGGCCAGA GTAAAGCACA GCGCGGGAGC 
CTGGAGCATA TGAAGCTGAT CCTGCGTGAT AAGGAGAAGG AGGTGGAATG CCAGCAGGAG 
CATATCCAGG AACTGCAGGA GCACAAGGCC CAGCTGGAGC AGCAGCTCCA GGGCTTACAC 
AGGAAGATGG GGGAGACCAG CCTTCTCCTG ACCCAGCGGG AGCAGGAGAT AGTGGCTCTG 
CAGCGGCACC TGCAGGAAGC CAGGGAACAG GGAGAGCTGA AAGAGCAGTC ACTTCAAGGT 
CAGCTGGAAG AGGCCCAGAG GGCGCTGGCC CGAAGGGACC AGGAGCTCAA GGCCCTGCAG 
CAGCAGCAGG CCCAAGGCCA GGAGGAAAAT GTGATGGAAA AGGCGGGCTT ACTGCAGAGA 
GAGCTGGAGC AGGCCCACGT GACACTGAAG GAGCGCCAGG CGGAGCTTGA GGATTACAAG 
GAGCATGTGA GAAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCAGCGGGT ACAGGCCCTG 
GAGGAGGTGT TGAGTGACCT AAGGGCTGAA TCTCGGGAAC AAGAGAAGGC CCTGCTGGCC 
CTCCAGCAGC AGTGTGCCGA GCAGGCCCAG GAGCACGAGG CAGAGGCCAG GGCCCTGCAG 
GACAGCTGGC TGCAGGCAGA GGCTATGCTC AAGGAACGGG ACCAGGAACT GGAGGCCCTG 
CGGGCAGAGG GCCTGTCTTC CCAGCATCGG GAGGAGGCTG CCTGTGGCCA GGTGGAGGCT 
CTGCAGGAGG CCCTCAGCAA GGCTCAGGCT GACCTGCAGG AGAAAGAGCA GCATCTCCTT 
GGGCAGGTGG AACTGAGCCG CAGCCTAGAG GCCAGCACGG CCACGTTGCA GGCTGCCCTG 
GACTCCTGCC AGGCACAGTC CCGGCAGCTG GAGGAGGCCC TGAGGAAGCG GGAAGGTGAG 
GTCCAGGTCC GGGACCTCCG ATACCAGGAG GCCATGCAGC AGCTCCAGCA GGCACTTACC 
CAGAGGGATG AAGAGCTGAG ACAACAGAGG AAACAGGGGC AGCTCCTGGA GAAGTCTCTG 
GCCCAGAGGG GCCAAGAGGA TGCAACCCAA GAGAAGCAAG AAGCAAAGCA CGAGAGAGAA 
GAGGAAGAGA TAAGGAGCCT TCGTGAGAGT CTGCAGGAGC TACAGCTGAC TCTAGCACAA 
AAGGAAGAGG AGATCCTGGA GCTGAGGGAG GCCCAGCAGA GGAAGAGTCT GGAGGACTTG 
TCTCACAAAG GCTGCCCAGG GAAGGAGCCG GCTACAACAT TTGATTCCTT AGGGCCCAGG 
CTGCAGCGGG AACTGGAGCG ACTACAGGCA GCACTGAAGC AGACAGAAGC CAGGGAGATC 
GAGTGGAGGG AGAAGGCCCA GGATCTGGCG CTGTCCCTGG CCCAGAGCAA GGCCAGTGTC 
AGCGATCTGC AGGAGGTAGC CCTGTTCCTG CAAGCATCTG TTCTGGAGCG GGACTCAGAA 
CAGCAGAGGC TGCAGGATGA GTTGGAGCTC ACCAAACTCA CTCTGGAGAA AGAGCGACTC 
CACAGCCCAG GCCCGAGCAG CAGAGCAGAA CGGGGGCCTA GAGGAGAGCC AGGTGTACAA 
CTGGGAGAGG TCTCGGGAGT GAAGACTGAG CCTAGTCCTG AGATGGAGGA GAGGCAGCTC 
TGGGAACAGA GGCTTGAGCA CCTGCAGCGA GCGGTGGCTC TCTTGGAGGT TGACCGAAGC 
CAGCTGCAAC AACATAACGC CCAGCTGCGG ACCACCCTGG AGCAGGTGGA GCGAGAGCGG 
AGGAAACTGA AGAGGGGTTC CATGCGTGCA TTGCGGGCAG GGGACCTGGA GAGCGGTGAA 
GCCACGGCAC CATCACCCAT GCAACAGGAT GGAAGAGGAA GGCAGAAGGG CTGCTCAGAT 
GCCAAGCACA TGGTTGAGCT GCAAAAAGAG GTGGCCCTCC TGCGAGCCCA GCTGGCCTTG 
GAGCGGAAGC AGAAGCAGGA CTACATTGCT CGCTCAGTTC AGACGAGCCG TGAGCTGGCG 
GACCTGCACC ACGGCCTCTC TCACTCCCTT CTCGCTGTAG CCCAAGCCCC CGAGGCCACC 
GTCTTAGAGG CCGAGACCCG CAAGCTGGAC GAGTCTCTGA CTCAAAGCCT GACATCCCCC 
GGGCCAGCAC TGCTGTGCCC CAGCTCCAGT GCTGCTCAGA CTGCCTCCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020933917.1
CDS	183..7136
Translation	MEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_021078258.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078258.1

ORF Insert Sequence:

ATGGAAAAAG CTGACGTGGT GAATAAAGCG CTTCGGGAAG ATGTGGAAAA ATTGACAGTA 
GACTGGAGCC AGGCCCGGGA TGAGCTAATG AGGAAGGAGA GCCAGTGGCA GATGGAGCAG 
GAGTTCTTCA AGGGCTACCT GAAAGGGGAG CATGGTCGCC TTCTCAGTCT ATGGCGGGAG 
GTGGTGACCT TCCGACGTCA CTTTCTGGAA ATGAAGTCAG CCACTGACAG AGACCTGATG 
GAGCTCAAGG CTGAGCACGT GAAGCTCTCT GGGTCCCTGT TGACCTGTTG TCTGCGCTTG 
ACTGTGGGAG CACCACAGGC CCGGGAATCC ACTGGATCTG GGAGGCTGAA TGGGAGTGAA 
CCATCCCAGC TGATGCTGCT ACTGTCTAAG ACCCAGGAGC TGGAGAAGGA AGCCCATAAA 
AGGAGCCAGG AGTTAATACA GCTTAAGAGT CAAGGGGATC TGGAGAAGGC TGAGCTTCAG 
GATCGGGTGA CCGAGCTTTC TGCTCTGCTG ACCCAGTCTC AGAAGCAGAA TGAAGATTAT 
GAAAAGATGG TAAAGGCTCT GAGAGAGACA ATGAAGATCC TGGAGGCAAA TCATGCAGAG 
TTAATGGAAC ATGAGGCATC TCTTAGTAGG AATGCCCAAG AGGAGAGGCT GTCTTTACAG 
CAAGTGATCA AGGATATAAC CCAGGCCATG GTAGAAGAAG GGGACCATAT GGCCCAAGGC 
TCTGGTCACG AGCACTCCTT GGACTTGGAT CCTAGTGGCT TCTTCTCCCA GTTTGATTCC 
CAGCAGCCAG ACAAGGCCCT CACTCTGGTG CGTTCTGTGT TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAAGCAGCA GCTTTCAGGC TGTCAGGAAG CTGTGAGCTC CTTGCAGCAG 
CAGCATCATC AGTGGGAAGA AGAGGGTGAG GCCTTGAGAC AGCGGCTGCA GAAGCTCACC 
GGGGAGCGGG ACACCCTGGC AGGGCAGACT GTGGGCCTGC AGGGGGAGGT GGACTCTCTG 
AGCAGGGAGC GAGAGCTCCT GCAAAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCCTGGCG ACTACGGAGG ACAAACATGG AGCTTCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAGCAGCAG GAGGAGCTGC ACCTGGCTGT CCGAGAACGG 
GAGCGCCTTC AGGAGATGCT GGCGAGCCTG GAAGCCAAAC AGTCAGAATC TCTCAGTGAA 
CTGATCACCC TTCGGGAAGC CCTGGAGTCT AGTCGCCTAG AAGGGGAGTT GCTGAGGCAA 
GAGCAAACAG AAGTGACTGC TGTGCTAGCA AAGGCAGAAC AGTCTATTGT GGAGCTGTCA 
AGTTCTGAGA ACAGCCTGAA GGCTGAGGTA GCTGATCTTC GGGCTGCAGC TGTCAAGCTC 
AGTGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGC AGAACCAGTC TGTGTGCAGC AGGATGGAGG CAGCAGAGCA GGCAAGAAGC 
GCTTTGCAGG TGGGCCTGGC AGAGGTGGAG AGGAGCAGGG AAGCGCTGTG GGAAAAGAAC 
ACTTACCTGG AGGCTCAGCT GCAAAAAGCA GAGGCGGCCA GGGCTGAGCT GCAGGCAGAT 
CTCAGGGGCG TCCAGGAAGA GAAGGAAGAA ATTCAAGAGA AACTAAGCGA GGCACATCAC 
CAGCAGGAGG CAGTCTCAGT TCAGATGGAG CAGCTGCGTC AGGAGGCAAA GCGGCAGGAA 
GAGGTGCTCG CCCGGGAAGT CCAGGAGAAG GAGGCTCTGG TGCGGGAAAA GGCGGCTCTT 
GAAGTGCGGC TGCAGGCTGT GGAGCGGGAC CGGCAGGACC TCTCTGAACA ACTGCTGGGG 
CTCAGCTCGG CCAAGGAGCT TCTGGAGAAC AGTCTGTTTG AGGCCCAGCA ACAGAATTCT 
CTGATAGAAG TCACCAAGGG GCAGCTGGAG GTCCAGATTC AAACTATCAC TCAAGCCAAG 
GAGGTAATCC AAGGGGAAGT GAGGTGCCTA CAGCTGGAAC TGGATACTGA ACGGAGCCGG 
GCAGAACAGG AGCGGGATGC TGCAACCAAA CAGCTGGCCC AGGCTGAGCA AGAGGGGCAG 
ACCGCCCTGC AGCAGCAGAA GTCAGCCCAT GAGGAGGAAG TGAAGGGGCT CCAGGAAAAA 
TGGGAGAAAG AGCGTGCCTG GCACCAGCAG GAACTGGATA AGGCTCTGAA GAGCCTTGAG 
AGGGAGAAAA CGGAGCTGGA AATGAGGCTG AGGGAGCAGC AGGCAGAAAC AGAGGCCATG 
CGGACGCAGA GGGAAGAAGA ACGGGCTGAG GCTGAGAGCA CCCTGCGCCA GATGCAGCTC 
GAAACAGAGA AGGAGAGAAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGATGCCA GCCAACAACT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GAAGTTAAAG 
GAGCAGGAGA CCACTGGGAT GCTGCAGACC CAGCTCCGGG AAGCTCAGAA GGAGCTGGAA 
CAGGCAGCCC AGCAGCACAG AGACAGCCTT GCTGCCCTCC AAGAAGAAGG CCGTGCCCTG 
CTGCAGGATA AGATAGACCT GCACAAGCAG GTGGAGGACT TGAAGTCTCA GCTGGTTTCC 
AAGGAAGACT CCCAAAGGCT GGTAGACCAG GAAGTTCAGG AGAAGCTGAG AGAGGCCCAG 
GAATGTAGCC GAATTCAGAA GGAGCTGGAG AAAGAAAAGG CCAGCCTGAC TCTGTTGCTG 
GTGGAAAAGG AACAGAGACT CCTTGGTTTA CAAGAGGCCG ACTCCATTCG ACAACAAGAG 
CTGAGCTCCC TGCGCCAGGA CATGCAGGAG GCCCAGGCAG GGCAGAAAGA GCTCAGCGCC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GCAAAGGAGA CTGACTTTCT GGCCCAGGAA 
GCGCGGCTGC TGGAAGAGCT GGAGGCATCT CAAGTAACAG AGCAGCAGCT ACGAGCTTCC 
TTGTGGGCCC AGGAAGCCAA GGCCGCACAA CTACAGCTGC GACTGCGCAG CACAGAGAGC 
CAGCTGGAGG TGCTGCTGGC AGAGCAGCAG CCTGGGCACC AGGCCCAGGC CCAGCTGGCC 
AGCCTTTGTG CTGTCCTGCA GCAGGCCCTG GGGTCTGTTT GTGAGAGCAG GCCTGAGCTG 
AGTGGCGGGG GAGACTCTGC TCCCTCCCTC TGGGGCCCTG AGCCAGACCA GAATGGAGCT 
AGGACCCTTT TCAAGAGAGG GCCCCTCCTG ACAGCTCTCT CGGCTGAGGC GGTGGCCTCT 
GCCCTTCACA AGCTTAACCA AGACCTGTGG AAGACTCAGC AGGCCCGGGA TGATCTGAGG 
GATCAGGCCC AGAAGCTGGG ACAACGTCTT ACTGAAACAG AGGCCGAGAA GAGCCAGCTC 
CACACAGAGT TGCAGGCTCT GCAGAGGCAG CTCTCCCAGA ACCAGGAAGA GAGATCCAAG 
TGGGAAGGAA AACAGACCTC CTTAGAATCT GAGCTGGTGG AACTGCATGA AACTGTGACA 
TCCTTACAGA GTCGCCTACG GCAAGCAGAG CTGCAGGGAA TAGAAGCCCA GAATGAGCAA 
GAGTTACTGC AGGCAGCCAA GGAGAACCTG ACAGCCCAGG TGGAACACCT CCAAGCAGCT 
GTTGCAGAAG CCAGGGCTCA GGCAAGCGCT GCCGAGGTCC TGGAGGAAGA CCTCAAAACT 
GCACGCTCAG CCCTGAAACT GAAAAGCAAG GAGGTGGAGA GTGAGCGTGA GCGAGCCCAG 
GCCCTACAGG AGCAGGGCGA GCTGAAGGTG GCCCAAGGGA AAGCTTTGCA GGAGAATTTG 
GCTCGCCTGA CTCAGACCCT ATCTGAGAGA GAAGGGGAGG TGGAAACTTT GCAGGGAAAA 
ATCCAGGAGC TGGAGAAGCA ACGGGAAATG CAAAAGGCTG CTTTGGAGGT GCTGTCTTTG 
GACCTCAAAA AGAGGAACCA AGAGGTGGAT CTGCAGCAAG AACAGATTCA GGAGCTGGAG 
GAGTGCAGAT CGGTTTTAGA GCATCTCCCC ATGGCAGTCC AGGAGCGAGA GCAGAAGCTG 
ACTCTGCAGA GGGAGCAGAT CAAGGAGCTC GAGAAGGACC GAGAGACACA GAGGAACATC 
TTGGAGCATC AGCTTCTGGA ACTTGAGAAG AAGGCCCAAG TGATTGAGTC CCAGAAAGGA 
CAGATTCAGG ATCTGAAGAA GCAGCTGGTT ACTCTGGAAT GCCTGGCCCT GGAACTAGAG 
GAAAACCATC ACAAAATAGA GTGCCAGCAA AAGACAATTG AGGAGCTGGA GGGCCAGAGG 
GAAATGCAGA GGGCAGCTCT GACCCATCTG ACACTGGACC TGGAGGAAAG GAGCCAGGAG 
CTGCAGGTAC AGGGCAGCCA GATCCGGGAG CTAGAGAGTC ACAACAGCCT CTTGGCAAGA 
GAGCTCCAGG AGAAGGACCA GGAGATGACG TCCCAGCAAG ACCAGACAGA GGAACTGCAG 
AGGCAGAAGG AGCATTTGAC ACAGGACCTC AAGAGGCGGG AACAGGAGAT AGTGCTGCTG 
AAGGAGAGGA TTCAGGTCTT GGAAGATCAG AGAACCCTAC AGACCAAGAT CCTAGAGGAG 
GACCTGGAGC AGATCAAAAT GTCCTTGAGG GAGCGAGGCC GGGACCTGGC CTCGCAGAGG 
CAGCTGATGC AGGAGCGGGC AGAGGAAGGG AAGGGCCAGA GTAAAGCACA GCGCGGGAGC 
CTGGAGCATA TGAAGCTGAT CCTGCGTGAT AAGGAGAAGG AGGTGGAATG CCAGCAGGAG 
CATATCCAGG AACTGCAGGA GCACAAGGCC CAGCTGGAGC AGCAGCTCCA GGGCTTACAC 
AGGAAGATGG GGGAGACCAG CCTTCTCCTG ACCCAGCGGG AGCAGGAGAT AGTGGCTCTG 
CAGCGGCACC TGCAGGAAGC CAGGGAACAG GGAGAGCTGA AAGAGCAGTC ACTTCAAGGT 
CAGCTGGAAG AGGCCCAGAG GGCGCTGGCC CGAAGGGACC AGGAGCTCAA GGCCCTGCAG 
CAGCAGCAGG CCCAAGGCCA GGAGGAAAAT GTGATGGAAA AGGCGGGCTT ACTGCAGAGA 
GAGCTGGAGC AGGCCCACGT GACACTGAAG GAGCGCCAGG CGGAGCTTGA GGATTACAAG 
GAGCATGTGA GAAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCAGCGGGT ACAGGCCCTG 
GAGGAGGTGT TGAGTGACCT AAGGGCTGAA TCTCGGGAAC AAGAGAAGGC CCTGCTGGCC 
CTCCAGCAGC AGTGTGCCGA GCAGGCCCAG GAGCACGAGG CAGAGGCCAG GGCCCTGCAG 
GACAGCTGGC TGCAGGCAGA GGCTATGCTC AAGGAACGGG ACCAGGAACT GGAGGCCCTG 
CGGGCAGAGG GCCTGTCTTC CCAGCATCGG GAGGAGGCTG CCTGTGGCCA GGTGGAGGCT 
CTGCAGGAGG CCCTCAGCAA GGCTCAGGCT GACCTGCAGG AGAAAGAGCA GCATCTCCTT 
GGGCAGGTGG AACTGAGCCG CAGCCTAGAG GCCAGCACGG CCACGTTGCA GGCTGCCCTG 
GACTCCTGCC AGGCACAGTC CCGGCAGCTG GAGGAGGCCC TGAGGAAGCG GGAAGGTGAG 
GTCCAGGTCC GGGACCTCCG ATACCAGGAG GCCATGCAGC AGCTCCAGCA GGCACTTACC 
CAGAGGGATG AAGAGCTGAG ACAACAGAGG AAACAGGGGC AGCTCCTGGA GAAGTCTCTG 
GCCCAGAGGG GCCAAGAGGA TGCAACCCAA GAGAAGCAAG AAGCAAAGCA CGAGAGAGAA 
GAGGAAGAGA TAAGGAGCCT TCGTGAGAGT CTGCAGGAGC TACAGCTGAC TCTAGCACAA 
AAGGAAGAGG AGATCCTGGA GCTGAGGGAG GCCCAGCAGA GGAAGAGTCT GGAGGACTTG 
TCTCACAAAG GCTGCCCAGG GAAGGAGCCG GCTACAACAT TTGATTCCTT AGGGCCCAGG 
CTGCAGCGGG AACTGGAGCG ACTACAGGCA GCACTGAAGC AGACAGAAGC CAGGGAGATC 
GAGTGGAGGG AGAAGGCCCA GGATCTGGCG CTGTCCCTGG CCCAGAGCAA GGCCAGTGTC 
AGCGATCTGC AGGAGGTAGC CCTGTTCCTG CAAGCATCTG TTCTGGAGCG GGACTCAGAA 
CAGCAGAGGC TGCAGGATGA GTTGGAGCTC ACCAAACTCA CTCTGGAGAA AGAGCGACTC 
CACAGCCCAG GCCCGAGCAG CAGAGCAGAA CGGGGGCCTA GAGGAGAGCC AGGTGTACAA 
CTGGGAGAGG TCTCGGGAGT GAAGACTGAG CCTAGTCCTG AGATGGAGGA GAGGCAGCTC 
TGGGAACAGA GGCTTGAGCA CCTGCAGCGA GCGGTGGCTC TCTTGGAGGT TGACCGAAGC 
CAGCTGCAAC AACATAACGC CCAGCTGCGG ACCACCCTGG AGCAGGTGGA GCGAGAGCGG 
AGGAAACTGA AGAGGGGTTC CATGCGTGCA TTGCGGGCAG GGGACCTGGA GAGCGGTGAA 
GCCACGGCAC CATCACCCAT GCAACAGGAT GGAAGAGGAA GGCAGAAGGG CTGCTCAGAT 
GCCAAGCACA TGGTTGAGCT GCAAAAAGAG GTGGCCCTCC TGCGAGCCCA GCTGGCCTTG 
GAGCGGAAGC AGAAGCAGGA CTACATTGCT CGCTCAGTTC AGACGAGCCG TGAGCTGGCG 
GACCTGCACC ACGGCCTCTC TCACTCCCTT CTCGCTGTAG CCCAAGCCCC CGAGGCCACC 
GTCTTAGAGG CCGAGACCCG CAAGCTGGAC GAGTCTCTGA CTCAAAGCCT GACATCCCCC 
GGGCCAGCAC TGCTGTGCCC CAGCTCCAGT GCTGCTCAGA CTGCCTCCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_021078259.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020933918.1 Sus scrofa centrosomal protein 250 (CEP250), transcript variant X8, mRNA.

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CloneID	OSe241634
Clone ID Related Accession (Same CDS sequence)	XM_021078259.1
Accession Version	XM_021078259.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	centrosome-associated protein CEP250 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010459.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCAG CCACTGACAG AGACCTGATG GAGCTCAAGG CTGAGCACGT GAAGCTCTCT 
GGGTCCCTGT TGACCTGTTG TCTGCGCTTG ACTGTGGGAG CACCACAGGC CCGGGAATCC 
ACTGGATCTG GGAGGCTGAA TGGGAGTGAA CCATCCCAGC TGATGCTGCT ACTGTCTAAG 
ACCCAGGAGC TGGAGAAGGA AGCCCATAAA AGGAGCCAGG AGTTAATACA GCTTAAGAGT 
CAAGGGGATC TGGAGAAGGC TGAGCTTCAG GATCGGGTGA CCGAGCTTTC TGCTCTGCTG 
ACCCAGTCTC AGAAGCAGAA TGAAGATTAT GAAAAGATGG TAAAGGCTCT GAGAGAGACA 
ATGAAGATCC TGGAGGCAAA TCATGCAGAG TTAATGGAAC ATGAGGCATC TCTTAGTAGG 
AATGCCCAAG AGGAGAGGCT GTCTTTACAG CAAGTGATCA AGGATATAAC CCAGGCCATG 
GTAGAAGAAG GGGACCATAT GGCCCAAGGC TCTGGTCACG AGCACTCCTT GGACTTGGAT 
CCTAGTGGCT TCTTCTCCCA GTTTGATTCC CAGCAGCCAG ACAAGGCCCT CACTCTGGTG 
CGTTCTGTGT TGACTCAGAG ACGCCAGGCT GTGCAGGACC TAAAGCAGCA GCTTTCAGGC 
TGTCAGGAAG CTGTGAGCTC CTTGCAGCAG CAGCATCATC AGTGGGAAGA AGAGGGTGAG 
GCCTTGAGAC AGCGGCTGCA GAAGCTCACC GGGGAGCGGG ACACCCTGGC AGGGCAGACT 
GTGGGCCTGC AGGGGGAGGT GGACTCTCTG AGCAGGGAGC GAGAGCTCCT GCAAAAGACC 
AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG CTGGAGCAGG AGGCCTGGCG ACTACGGAGG 
ACAAACATGG AGCTTCAGCT GCAGGGCGAC TCTGCTCAGG GCGAGAAGGA GGAGCAGCAG 
GAGGAGCTGC ACCTGGCTGT CCGAGAACGG GAGCGCCTTC AGGAGATGCT GGCGAGCCTG 
GAAGCCAAAC AGTCAGAATC TCTCAGTGAA CTGATCACCC TTCGGGAAGC CCTGGAGTCT 
AGTCGCCTAG AAGGGGAGTT GCTGAGGCAA GAGCAAACAG AAGTGACTGC TGTGCTAGCA 
AAGGCAGAAC AGTCTATTGT GGAGCTGTCA AGTTCTGAGA ACAGCCTGAA GGCTGAGGTA 
GCTGATCTTC GGGCTGCAGC TGTCAAGCTC AGTGCCTTAA ATGAGGCTTT GGCCTTAGAT 
AAAGTTGGAC TGAACCAGCA GCTTCTCCAG TTAGAACAGC AGAACCAGTC TGTGTGCAGC 
AGGATGGAGG CAGCAGAGCA GGCAAGAAGC GCTTTGCAGG TGGGCCTGGC AGAGGTGGAG 
AGGAGCAGGG AAGCGCTGTG GGAAAAGAAC ACTTACCTGG AGGCTCAGCT GCAAAAAGCA 
GAGGCGGCCA GGGCTGAGCT GCAGGCAGAT CTCAGGGGCG TCCAGGAAGA GAAGGAAGAA 
ATTCAAGAGA AACTAAGCGA GGCACATCAC CAGCAGGAGG CAGTCTCAGT TCAGATGGAG 
CAGCTGCGTC AGGAGGCAAA GCGGCAGGAA GAGGTGCTCG CCCGGGAAGT CCAGGAGAAG 
GAGGCTCTGG TGCGGGAAAA GGCGGCTCTT GAAGTGCGGC TGCAGGCTGT GGAGCGGGAC 
CGGCAGGACC TCTCTGAACA ACTGCTGGGG CTCAGCTCGG CCAAGGAGCT TCTGGAGAAC 
AGTCTGTTTG AGGCCCAGCA ACAGAATTCT CTGATAGAAG TCACCAAGGG GCAGCTGGAG 
GTCCAGATTC AAACTATCAC TCAAGCCAAG GAGGTAATCC AAGGGGAAGT GAGGTGCCTA 
CAGCTGGAAC TGGATACTGA ACGGAGCCGG GCAGAACAGG AGCGGGATGC TGCAACCAAA 
CAGCTGGCCC AGGCTGAGCA AGAGGGGCAG ACCGCCCTGC AGCAGCAGAA GTCAGCCCAT 
GAGGAGGAAG TGAAGGGGCT CCAGGAAAAA TGGGAGAAAG AGCGTGCCTG GCACCAGCAG 
GAACTGGATA AGGCTCTGAA GAGCCTTGAG AGGGAGAAAA CGGAGCTGGA AATGAGGCTG 
AGGGAGCAGC AGGCAGAAAC AGAGGCCATG CGGACGCAGA GGGAAGAAGA ACGGGCTGAG 
GCTGAGAGCA CCCTGCGCCA GATGCAGCTC GAAACAGAGA AGGAGAGAAT GTCCCTCCTG 
GAGACACTGC TGCAGACCCA GAAGGAGCTG GCAGATGCCA GCCAACAACT GGAGCGGCTG 
AGGCAGGACA TGAAGGTTCA GAAGTTAAAG GAGCAGGAGA CCACTGGGAT GCTGCAGACC 
CAGCTCCGGG AAGCTCAGAA GGAGCTGGAA CAGGCAGCCC AGCAGCACAG AGACAGCCTT 
GCTGCCCTCC AAGAAGAAGG CCGTGCCCTG CTGCAGGATA AGATAGACCT GCACAAGCAG 
GTGGAGGACT TGAAGTCTCA GCTGGTTTCC AAGGAAGACT CCCAAAGGCT GGTAGACCAG 
GAAGTTCAGG AGAAGCTGAG AGAGGCCCAG GAATGTAGCC GAATTCAGAA GGAGCTGGAG 
AAAGAAAAGG CCAGCCTGAC TCTGTTGCTG GTGGAAAAGG AACAGAGACT CCTTGGTTTA 
CAAGAGGCCG ACTCCATTCG ACAACAAGAG CTGAGCTCCC TGCGCCAGGA CATGCAGGAG 
GCCCAGGCAG GGCAGAAAGA GCTCAGCGCC CAGGTGGAAT TACTGAAGCA GGAGGTGAAG 
GCAAAGGAGA CTGACTTTCT GGCCCAGGAA GCGCGGCTGC TGGAAGAGCT GGAGGCATCT 
CAAGTAACAG AGCAGCAGCT ACGAGCTTCC TTGTGGGCCC AGGAAGCCAA GGCCGCACAA 
CTACAGCTGC GACTGCGCAG CACAGAGAGC CAGCTGGAGG TGCTGCTGGC AGAGCAGCAG 
CCTGGGCACC AGGCCCAGGC CCAGCTGGCC AGCCTTTGTG CTGTCCTGCA GCAGGCCCTG 
GGGTCTGTTT GTGAGAGCAG GCCTGAGCTG AGTGGCGGGG GAGACTCTGC TCCCTCCCTC 
TGGGGCCCTG AGCCAGACCA GAATGGAGCT AGGACCCTTT TCAAGAGAGG GCCCCTCCTG 
ACAGCTCTCT CGGCTGAGGC GGTGGCCTCT GCCCTTCACA AGCTTAACCA AGACCTGTGG 
AAGACTCAGC AGGCCCGGGA TGATCTGAGG GATCAGGCCC AGAAGCTGGG ACAACGTCTT 
ACTGAAACAG AGGCCGAGAA GAGCCAGCTC CACACAGAGT TGCAGGCTCT GCAGAGGCAG 
CTCTCCCAGA ACCAGGAAGA GAGATCCAAG TGGGAAGGAA AACAGACCTC CTTAGAATCT 
GAGCTGGTGG AACTGCATGA AACTGTGACA TCCTTACAGA GTCGCCTACG GCAAGCAGAG 
CTGCAGGGAA TAGAAGCCCA GAATGAGCAA GAGTTACTGC AGGCAGCCAA GGAGAACCTG 
ACAGCCCAGG TGGAACACCT CCAAGCAGCT GTTGCAGAAG CCAGGGCTCA GGCAAGCGCT 
GCCGAGGTCC TGGAGGAAGA CCTCAAAACT GCACGCTCAG CCCTGAAACT GAAAAGCAAG 
GAGGTGGAGA GTGAGCGTGA GCGAGCCCAG GCCCTACAGG AGCAGGGCGA GCTGAAGGTG 
GCCCAAGGGA AAGCTTTGCA GGAGAATTTG GCTCGCCTGA CTCAGACCCT ATCTGAGAGA 
GAAGGGGAGG TGGAAACTTT GCAGGGAAAA ATCCAGGAGC TGGAGAAGCA ACGGGAAATG 
CAAAAGGCTG CTTTGGAGGT GCTGTCTTTG GACCTCAAAA AGAGGAACCA AGAGGTGGAT 
CTGCAGCAAG AACAGATTCA GGAGCTGGAG GAGTGCAGAT CGGTTTTAGA GCATCTCCCC 
ATGGCAGTCC AGGAGCGAGA GCAGAAGCTG ACTCTGCAGA GGGAGCAGAT CAAGGAGCTC 
GAGAAGGACC GAGAGACACA GAGGAACATC TTGGAGCATC AGCTTCTGGA ACTTGAGAAG 
AAGGCCCAAG TGATTGAGTC CCAGAAAGGA CAGATTCAGG ATCTGAAGAA GCAGCTGGTT 
ACTCTGGAAT GCCTGGCCCT GGAACTAGAG GAAAACCATC ACAAAATAGA GTGCCAGCAA 
AAGACAATTG AGGAGCTGGA GGGCCAGAGG GAAATGCAGA GGGCAGCTCT GACCCATCTG 
ACACTGGACC TGGAGGAAAG GAGCCAGGAG CTGCAGGTAC AGGGCAGCCA GATCCGGGAG 
CTAGAGAGTC ACAACAGCCT CTTGGCAAGA GAGCTCCAGG AGAAGGACCA GGAGATGACG 
TCCCAGCAAG ACCAGACAGA GGAACTGCAG AGGCAGAAGG AGCATTTGAC ACAGGACCTC 
AAGAGGCGGG AACAGGAGAT AGTGCTGCTG AAGGAGAGGA TTCAGGTCTT GGAAGATCAG 
AGAACCCTAC AGACCAAGAT CCTAGAGGAG GACCTGGAGC AGATCAAAAT GTCCTTGAGG 
GAGCGAGGCC GGGACCTGGC CTCGCAGAGG CAGCTGATGC AGGAGCGGGC AGAGGAAGGG 
AAGGGCCAGA GTAAAGCACA GCGCGGGAGC CTGGAGCATA TGAAGCTGAT CCTGCGTGAT 
AAGGAGAAGG AGGTGGAATG CCAGCAGGAG CATATCCAGG AACTGCAGGA GCACAAGGCC 
CAGCTGGAGC AGCAGCTCCA GGGCTTACAC AGGAAGATGG GGGAGACCAG CCTTCTCCTG 
ACCCAGCGGG AGCAGGAGAT AGTGGCTCTG CAGCGGCACC TGCAGGAAGC CAGGGAACAG 
GGAGAGCTGA AAGAGCAGTC ACTTCAAGGT CAGCTGGAAG AGGCCCAGAG GGCGCTGGCC 
CGAAGGGACC AGGAGCTCAA GGCCCTGCAG CAGCAGCAGG CCCAAGGCCA GGAGGAAAAT 
GTGATGGAAA AGGCGGGCTT ACTGCAGAGA GAGCTGGAGC AGGCCCACGT GACACTGAAG 
GAGCGCCAGG CGGAGCTTGA GGATTACAAG GAGCATGTGA GAAGGCTCCA GGAAGAGCTG 
GCAGTGGAGG GGCAGCGGGT ACAGGCCCTG GAGGAGGTGT TGAGTGACCT AAGGGCTGAA 
TCTCGGGAAC AAGAGAAGGC CCTGCTGGCC CTCCAGCAGC AGTGTGCCGA GCAGGCCCAG 
GAGCACGAGG CAGAGGCCAG GGCCCTGCAG GACAGCTGGC TGCAGGCAGA GGCTATGCTC 
AAGGAACGGG ACCAGGAACT GGAGGCCCTG CGGGCAGAGG GCCTGTCTTC CCAGCATCGG 
GAGGAGGCTG CCTGTGGCCA GGTGGAGGCT CTGCAGGAGG CCCTCAGCAA GGCTCAGGCT 
GACCTGCAGG AGAAAGAGCA GCATCTCCTT GGGCAGGTGG AACTGAGCCG CAGCCTAGAG 
GCCAGCACGG CCACGTTGCA GGCTGCCCTG GACTCCTGCC AGGCACAGTC CCGGCAGCTG 
GAGGAGGCCC TGAGGAAGCG GGAAGGTGAG GTCCAGGTCC GGGACCTCCG ATACCAGGAG 
GCCATGCAGC AGCTCCAGCA GGCACTTACC CAGAGGGATG AAGAGCTGAG ACAACAGAGG 
AAACAGGGGC AGCTCCTGGA GAAGTCTCTG GCCCAGAGGG GCCAAGAGGA TGCAACCCAA 
GAGAAGCAAG AAGCAAAGCA CGAGAGAGAA GAGGAAGAGA TAAGGAGCCT TCGTGAGAGT 
CTGCAGGAGC TACAGCTGAC TCTAGCACAA AAGGAAGAGG AGATCCTGGA GCTGAGGGAG 
GCCCAGCAGA GGAAGAGTCT GGAGGACTTG TCTCACAAAG GCTGCCCAGG GAAGGAGCCG 
GCTACAACAT TTGATTCCTT AGGGCCCAGG CTGCAGCGGG AACTGGAGCG ACTACAGGCA 
GCACTGAAGC AGACAGAAGC CAGGGAGATC GAGTGGAGGG AGAAGGCCCA GGATCTGGCG 
CTGTCCCTGG CCCAGAGCAA GGCCAGTGTC AGCGATCTGC AGGAGGTAGC CCTGTTCCTG 
CAAGCATCTG TTCTGGAGCG GGACTCAGAA CAGCAGAGGC TGCAGGATGA GTTGGAGCTC 
ACCAAACTCA CTCTGGAGAA AGAGCGACTC CACAGCCCAG GCCCGAGCAG CAGAGCAGAA 
CGGGGGCCTA GAGGAGAGCC AGGTGTACAA CTGGGAGAGG TCTCGGGAGT GAAGACTGAG 
CCTAGTCCTG AGATGGAGGA GAGGCAGCTC TGGGAACAGA GGCTTGAGCA CCTGCAGCGA 
GCGGTGGCTC TCTTGGAGGT TGACCGAAGC CAGCTGCAAC AACATAACGC CCAGCTGCGG 
ACCACCCTGG AGCAGGTGGA GCGAGAGCGG AGGAAACTGA AGAGGGGTTC CATGCGTGCA 
TTGCGGGCAG GGGACCTGGA GAGCGGTGAA GCCACGGCAC CATCACCCAT GCAACAGGAT 
GGAAGAGGAA GGCAGAAGGG CTGCTCAGAT GCCAAGCACA TGGTTGAGCT GCAAAAAGAG 
GTGGCCCTCC TGCGAGCCCA GCTGGCCTTG GAGCGGAAGC AGAAGCAGGA CTACATTGCT 
CGCTCAGTTC AGACGAGCCG TGAGCTGGCG GACCTGCACC ACGGCCTCTC TCACTCCCTT 
CTCGCTGTAG CCCAAGCCCC CGAGGCCACC GTCTTAGAGG CCGAGACCCG CAAGCTGGAC 
GAGTCTCTGA CTCAAAGCCT GACATCCCCC GGGCCAGCAC TGCTGTGCCC CAGCTCCAGT 
GCTGCTCAGA CTGCCTCCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020933918.1
CDS	1834..8577
Translation	MKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_021078259.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078259.1

ORF Insert Sequence:

ATGAAGTCAG CCACTGACAG AGACCTGATG GAGCTCAAGG CTGAGCACGT GAAGCTCTCT 
GGGTCCCTGT TGACCTGTTG TCTGCGCTTG ACTGTGGGAG CACCACAGGC CCGGGAATCC 
ACTGGATCTG GGAGGCTGAA TGGGAGTGAA CCATCCCAGC TGATGCTGCT ACTGTCTAAG 
ACCCAGGAGC TGGAGAAGGA AGCCCATAAA AGGAGCCAGG AGTTAATACA GCTTAAGAGT 
CAAGGGGATC TGGAGAAGGC TGAGCTTCAG GATCGGGTGA CCGAGCTTTC TGCTCTGCTG 
ACCCAGTCTC AGAAGCAGAA TGAAGATTAT GAAAAGATGG TAAAGGCTCT GAGAGAGACA 
ATGAAGATCC TGGAGGCAAA TCATGCAGAG TTAATGGAAC ATGAGGCATC TCTTAGTAGG 
AATGCCCAAG AGGAGAGGCT GTCTTTACAG CAAGTGATCA AGGATATAAC CCAGGCCATG 
GTAGAAGAAG GGGACCATAT GGCCCAAGGC TCTGGTCACG AGCACTCCTT GGACTTGGAT 
CCTAGTGGCT TCTTCTCCCA GTTTGATTCC CAGCAGCCAG ACAAGGCCCT CACTCTGGTG 
CGTTCTGTGT TGACTCAGAG ACGCCAGGCT GTGCAGGACC TAAAGCAGCA GCTTTCAGGC 
TGTCAGGAAG CTGTGAGCTC CTTGCAGCAG CAGCATCATC AGTGGGAAGA AGAGGGTGAG 
GCCTTGAGAC AGCGGCTGCA GAAGCTCACC GGGGAGCGGG ACACCCTGGC AGGGCAGACT 
GTGGGCCTGC AGGGGGAGGT GGACTCTCTG AGCAGGGAGC GAGAGCTCCT GCAAAAGACC 
AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG CTGGAGCAGG AGGCCTGGCG ACTACGGAGG 
ACAAACATGG AGCTTCAGCT GCAGGGCGAC TCTGCTCAGG GCGAGAAGGA GGAGCAGCAG 
GAGGAGCTGC ACCTGGCTGT CCGAGAACGG GAGCGCCTTC AGGAGATGCT GGCGAGCCTG 
GAAGCCAAAC AGTCAGAATC TCTCAGTGAA CTGATCACCC TTCGGGAAGC CCTGGAGTCT 
AGTCGCCTAG AAGGGGAGTT GCTGAGGCAA GAGCAAACAG AAGTGACTGC TGTGCTAGCA 
AAGGCAGAAC AGTCTATTGT GGAGCTGTCA AGTTCTGAGA ACAGCCTGAA GGCTGAGGTA 
GCTGATCTTC GGGCTGCAGC TGTCAAGCTC AGTGCCTTAA ATGAGGCTTT GGCCTTAGAT 
AAAGTTGGAC TGAACCAGCA GCTTCTCCAG TTAGAACAGC AGAACCAGTC TGTGTGCAGC 
AGGATGGAGG CAGCAGAGCA GGCAAGAAGC GCTTTGCAGG TGGGCCTGGC AGAGGTGGAG 
AGGAGCAGGG AAGCGCTGTG GGAAAAGAAC ACTTACCTGG AGGCTCAGCT GCAAAAAGCA 
GAGGCGGCCA GGGCTGAGCT GCAGGCAGAT CTCAGGGGCG TCCAGGAAGA GAAGGAAGAA 
ATTCAAGAGA AACTAAGCGA GGCACATCAC CAGCAGGAGG CAGTCTCAGT TCAGATGGAG 
CAGCTGCGTC AGGAGGCAAA GCGGCAGGAA GAGGTGCTCG CCCGGGAAGT CCAGGAGAAG 
GAGGCTCTGG TGCGGGAAAA GGCGGCTCTT GAAGTGCGGC TGCAGGCTGT GGAGCGGGAC 
CGGCAGGACC TCTCTGAACA ACTGCTGGGG CTCAGCTCGG CCAAGGAGCT TCTGGAGAAC 
AGTCTGTTTG AGGCCCAGCA ACAGAATTCT CTGATAGAAG TCACCAAGGG GCAGCTGGAG 
GTCCAGATTC AAACTATCAC TCAAGCCAAG GAGGTAATCC AAGGGGAAGT GAGGTGCCTA 
CAGCTGGAAC TGGATACTGA ACGGAGCCGG GCAGAACAGG AGCGGGATGC TGCAACCAAA 
CAGCTGGCCC AGGCTGAGCA AGAGGGGCAG ACCGCCCTGC AGCAGCAGAA GTCAGCCCAT 
GAGGAGGAAG TGAAGGGGCT CCAGGAAAAA TGGGAGAAAG AGCGTGCCTG GCACCAGCAG 
GAACTGGATA AGGCTCTGAA GAGCCTTGAG AGGGAGAAAA CGGAGCTGGA AATGAGGCTG 
AGGGAGCAGC AGGCAGAAAC AGAGGCCATG CGGACGCAGA GGGAAGAAGA ACGGGCTGAG 
GCTGAGAGCA CCCTGCGCCA GATGCAGCTC GAAACAGAGA AGGAGAGAAT GTCCCTCCTG 
GAGACACTGC TGCAGACCCA GAAGGAGCTG GCAGATGCCA GCCAACAACT GGAGCGGCTG 
AGGCAGGACA TGAAGGTTCA GAAGTTAAAG GAGCAGGAGA CCACTGGGAT GCTGCAGACC 
CAGCTCCGGG AAGCTCAGAA GGAGCTGGAA CAGGCAGCCC AGCAGCACAG AGACAGCCTT 
GCTGCCCTCC AAGAAGAAGG CCGTGCCCTG CTGCAGGATA AGATAGACCT GCACAAGCAG 
GTGGAGGACT TGAAGTCTCA GCTGGTTTCC AAGGAAGACT CCCAAAGGCT GGTAGACCAG 
GAAGTTCAGG AGAAGCTGAG AGAGGCCCAG GAATGTAGCC GAATTCAGAA GGAGCTGGAG 
AAAGAAAAGG CCAGCCTGAC TCTGTTGCTG GTGGAAAAGG AACAGAGACT CCTTGGTTTA 
CAAGAGGCCG ACTCCATTCG ACAACAAGAG CTGAGCTCCC TGCGCCAGGA CATGCAGGAG 
GCCCAGGCAG GGCAGAAAGA GCTCAGCGCC CAGGTGGAAT TACTGAAGCA GGAGGTGAAG 
GCAAAGGAGA CTGACTTTCT GGCCCAGGAA GCGCGGCTGC TGGAAGAGCT GGAGGCATCT 
CAAGTAACAG AGCAGCAGCT ACGAGCTTCC TTGTGGGCCC AGGAAGCCAA GGCCGCACAA 
CTACAGCTGC GACTGCGCAG CACAGAGAGC CAGCTGGAGG TGCTGCTGGC AGAGCAGCAG 
CCTGGGCACC AGGCCCAGGC CCAGCTGGCC AGCCTTTGTG CTGTCCTGCA GCAGGCCCTG 
GGGTCTGTTT GTGAGAGCAG GCCTGAGCTG AGTGGCGGGG GAGACTCTGC TCCCTCCCTC 
TGGGGCCCTG AGCCAGACCA GAATGGAGCT AGGACCCTTT TCAAGAGAGG GCCCCTCCTG 
ACAGCTCTCT CGGCTGAGGC GGTGGCCTCT GCCCTTCACA AGCTTAACCA AGACCTGTGG 
AAGACTCAGC AGGCCCGGGA TGATCTGAGG GATCAGGCCC AGAAGCTGGG ACAACGTCTT 
ACTGAAACAG AGGCCGAGAA GAGCCAGCTC CACACAGAGT TGCAGGCTCT GCAGAGGCAG 
CTCTCCCAGA ACCAGGAAGA GAGATCCAAG TGGGAAGGAA AACAGACCTC CTTAGAATCT 
GAGCTGGTGG AACTGCATGA AACTGTGACA TCCTTACAGA GTCGCCTACG GCAAGCAGAG 
CTGCAGGGAA TAGAAGCCCA GAATGAGCAA GAGTTACTGC AGGCAGCCAA GGAGAACCTG 
ACAGCCCAGG TGGAACACCT CCAAGCAGCT GTTGCAGAAG CCAGGGCTCA GGCAAGCGCT 
GCCGAGGTCC TGGAGGAAGA CCTCAAAACT GCACGCTCAG CCCTGAAACT GAAAAGCAAG 
GAGGTGGAGA GTGAGCGTGA GCGAGCCCAG GCCCTACAGG AGCAGGGCGA GCTGAAGGTG 
GCCCAAGGGA AAGCTTTGCA GGAGAATTTG GCTCGCCTGA CTCAGACCCT ATCTGAGAGA 
GAAGGGGAGG TGGAAACTTT GCAGGGAAAA ATCCAGGAGC TGGAGAAGCA ACGGGAAATG 
CAAAAGGCTG CTTTGGAGGT GCTGTCTTTG GACCTCAAAA AGAGGAACCA AGAGGTGGAT 
CTGCAGCAAG AACAGATTCA GGAGCTGGAG GAGTGCAGAT CGGTTTTAGA GCATCTCCCC 
ATGGCAGTCC AGGAGCGAGA GCAGAAGCTG ACTCTGCAGA GGGAGCAGAT CAAGGAGCTC 
GAGAAGGACC GAGAGACACA GAGGAACATC TTGGAGCATC AGCTTCTGGA ACTTGAGAAG 
AAGGCCCAAG TGATTGAGTC CCAGAAAGGA CAGATTCAGG ATCTGAAGAA GCAGCTGGTT 
ACTCTGGAAT GCCTGGCCCT GGAACTAGAG GAAAACCATC ACAAAATAGA GTGCCAGCAA 
AAGACAATTG AGGAGCTGGA GGGCCAGAGG GAAATGCAGA GGGCAGCTCT GACCCATCTG 
ACACTGGACC TGGAGGAAAG GAGCCAGGAG CTGCAGGTAC AGGGCAGCCA GATCCGGGAG 
CTAGAGAGTC ACAACAGCCT CTTGGCAAGA GAGCTCCAGG AGAAGGACCA GGAGATGACG 
TCCCAGCAAG ACCAGACAGA GGAACTGCAG AGGCAGAAGG AGCATTTGAC ACAGGACCTC 
AAGAGGCGGG AACAGGAGAT AGTGCTGCTG AAGGAGAGGA TTCAGGTCTT GGAAGATCAG 
AGAACCCTAC AGACCAAGAT CCTAGAGGAG GACCTGGAGC AGATCAAAAT GTCCTTGAGG 
GAGCGAGGCC GGGACCTGGC CTCGCAGAGG CAGCTGATGC AGGAGCGGGC AGAGGAAGGG 
AAGGGCCAGA GTAAAGCACA GCGCGGGAGC CTGGAGCATA TGAAGCTGAT CCTGCGTGAT 
AAGGAGAAGG AGGTGGAATG CCAGCAGGAG CATATCCAGG AACTGCAGGA GCACAAGGCC 
CAGCTGGAGC AGCAGCTCCA GGGCTTACAC AGGAAGATGG GGGAGACCAG CCTTCTCCTG 
ACCCAGCGGG AGCAGGAGAT AGTGGCTCTG CAGCGGCACC TGCAGGAAGC CAGGGAACAG 
GGAGAGCTGA AAGAGCAGTC ACTTCAAGGT CAGCTGGAAG AGGCCCAGAG GGCGCTGGCC 
CGAAGGGACC AGGAGCTCAA GGCCCTGCAG CAGCAGCAGG CCCAAGGCCA GGAGGAAAAT 
GTGATGGAAA AGGCGGGCTT ACTGCAGAGA GAGCTGGAGC AGGCCCACGT GACACTGAAG 
GAGCGCCAGG CGGAGCTTGA GGATTACAAG GAGCATGTGA GAAGGCTCCA GGAAGAGCTG 
GCAGTGGAGG GGCAGCGGGT ACAGGCCCTG GAGGAGGTGT TGAGTGACCT AAGGGCTGAA 
TCTCGGGAAC AAGAGAAGGC CCTGCTGGCC CTCCAGCAGC AGTGTGCCGA GCAGGCCCAG 
GAGCACGAGG CAGAGGCCAG GGCCCTGCAG GACAGCTGGC TGCAGGCAGA GGCTATGCTC 
AAGGAACGGG ACCAGGAACT GGAGGCCCTG CGGGCAGAGG GCCTGTCTTC CCAGCATCGG 
GAGGAGGCTG CCTGTGGCCA GGTGGAGGCT CTGCAGGAGG CCCTCAGCAA GGCTCAGGCT 
GACCTGCAGG AGAAAGAGCA GCATCTCCTT GGGCAGGTGG AACTGAGCCG CAGCCTAGAG 
GCCAGCACGG CCACGTTGCA GGCTGCCCTG GACTCCTGCC AGGCACAGTC CCGGCAGCTG 
GAGGAGGCCC TGAGGAAGCG GGAAGGTGAG GTCCAGGTCC GGGACCTCCG ATACCAGGAG 
GCCATGCAGC AGCTCCAGCA GGCACTTACC CAGAGGGATG AAGAGCTGAG ACAACAGAGG 
AAACAGGGGC AGCTCCTGGA GAAGTCTCTG GCCCAGAGGG GCCAAGAGGA TGCAACCCAA 
GAGAAGCAAG AAGCAAAGCA CGAGAGAGAA GAGGAAGAGA TAAGGAGCCT TCGTGAGAGT 
CTGCAGGAGC TACAGCTGAC TCTAGCACAA AAGGAAGAGG AGATCCTGGA GCTGAGGGAG 
GCCCAGCAGA GGAAGAGTCT GGAGGACTTG TCTCACAAAG GCTGCCCAGG GAAGGAGCCG 
GCTACAACAT TTGATTCCTT AGGGCCCAGG CTGCAGCGGG AACTGGAGCG ACTACAGGCA 
GCACTGAAGC AGACAGAAGC CAGGGAGATC GAGTGGAGGG AGAAGGCCCA GGATCTGGCG 
CTGTCCCTGG CCCAGAGCAA GGCCAGTGTC AGCGATCTGC AGGAGGTAGC CCTGTTCCTG 
CAAGCATCTG TTCTGGAGCG GGACTCAGAA CAGCAGAGGC TGCAGGATGA GTTGGAGCTC 
ACCAAACTCA CTCTGGAGAA AGAGCGACTC CACAGCCCAG GCCCGAGCAG CAGAGCAGAA 
CGGGGGCCTA GAGGAGAGCC AGGTGTACAA CTGGGAGAGG TCTCGGGAGT GAAGACTGAG 
CCTAGTCCTG AGATGGAGGA GAGGCAGCTC TGGGAACAGA GGCTTGAGCA CCTGCAGCGA 
GCGGTGGCTC TCTTGGAGGT TGACCGAAGC CAGCTGCAAC AACATAACGC CCAGCTGCGG 
ACCACCCTGG AGCAGGTGGA GCGAGAGCGG AGGAAACTGA AGAGGGGTTC CATGCGTGCA 
TTGCGGGCAG GGGACCTGGA GAGCGGTGAA GCCACGGCAC CATCACCCAT GCAACAGGAT 
GGAAGAGGAA GGCAGAAGGG CTGCTCAGAT GCCAAGCACA TGGTTGAGCT GCAAAAAGAG 
GTGGCCCTCC TGCGAGCCCA GCTGGCCTTG GAGCGGAAGC AGAAGCAGGA CTACATTGCT 
CGCTCAGTTC AGACGAGCCG TGAGCTGGCG GACCTGCACC ACGGCCTCTC TCACTCCCTT 
CTCGCTGTAG CCCAAGCCCC CGAGGCCACC GTCTTAGAGG CCGAGACCCG CAAGCTGGAC 
GAGTCTCTGA CTCAAAGCCT GACATCCCCC GGGCCAGCAC TGCTGTGCCC CAGCTCCAGT 
GCTGCTCAGA CTGCCTCCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_021078261.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020933920.1 Sus scrofa centrosomal protein 250 (CEP250), transcript variant X10, mRNA.

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CloneID	OSe241635
Clone ID Related Accession (Same CDS sequence)	XM_021078261.1
Accession Version	XM_021078261.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6474bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	centrosome-associated protein CEP250 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010459.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTGA CCGAGCTTTC TGCTCTGCTG ACCCAGTCTC AGAAGCAGAA TGAAGATTAT 
GAAAAGATGG TAAAGGCTCT GAGAGAGACA ATGAAGATCC TGGAGGCAAA TCATGCAGAG 
TTAATGGAAC ATGAGGCATC TCTTAGTAGG AATGCCCAAG AGGAGAGGCT GTCTTTACAG 
CAAGTGATCA AGGATATAAC CCAGGCCATG GTAGAAGAAG GGGACCATAT GGCCCAAGGC 
TCTGGTCACG AGCACTCCTT GGACTTGGAT CCTAGTGGCT TCTTCTCCCA GTTTGATTCC 
CAGCAGCCAG ACAAGGCCCT CACTCTGGTG CGTTCTGTGT TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAAGCAGCA GCTTTCAGGC TGTCAGGAAG CTGTGAGCTC CTTGCAGCAG 
CAGCATCATC AGTGGGAAGA AGAGGGTGAG GCCTTGAGAC AGCGGCTGCA GAAGCTCACC 
GGGGAGCGGG ACACCCTGGC AGGGCAGACT GTGGGCCTGC AGGGGGAGGT GGACTCTCTG 
AGCAGGGAGC GAGAGCTCCT GCAAAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCCTGGCG ACTACGGAGG ACAAACATGG AGCTTCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAGCAGCAG GAGGAGCTGC ACCTGGCTGT CCGAGAACGG 
GAGCGCCTTC AGGAGATGCT GGCGAGCCTG GAAGCCAAAC AGTCAGAATC TCTCAGTGAA 
CTGATCACCC TTCGGGAAGC CCTGGAGTCT AGTCGCCTAG AAGGGGAGTT GCTGAGGCAA 
GAGCAAACAG AAGTGACTGC TGTGCTAGCA AAGGCAGAAC AGTCTATTGT GGAGCTGTCA 
AGTTCTGAGA ACAGCCTGAA GGCTGAGGTA GCTGATCTTC GGGCTGCAGC TGTCAAGCTC 
AGTGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGC AGAACCAGTC TGTGTGCAGC AGGATGGAGG CAGCAGAGCA GGCAAGAAGC 
GCTTTGCAGG TGGGCCTGGC AGAGGTGGAG AGGAGCAGGG AAGCGCTGTG GGAAAAGAAC 
ACTTACCTGG AGGCTCAGCT GCAAAAAGCA GAGGCGGCCA GGGCTGAGCT GCAGGCAGAT 
CTCAGGGGCG TCCAGGAAGA GAAGGAAGAA ATTCAAGAGA AACTAAGCGA GGCACATCAC 
CAGCAGGAGG CAGTCTCAGT TCAGATGGAG CAGCTGCGTC AGGAGGCAAA GCGGCAGGAA 
GAGGTGCTCG CCCGGGAAGT CCAGGAGAAG GAGGCTCTGG TGCGGGAAAA GGCGGCTCTT 
GAAGTGCGGC TGCAGGCTGT GGAGCGGGAC CGGCAGGACC TCTCTGAACA ACTGCTGGGG 
CTCAGCTCGG CCAAGGAGCT TCTGGAGAAC AGTCTGTTTG AGGCCCAGCA ACAGAATTCT 
CTGATAGAAG TCACCAAGGG GCAGCTGGAG GTCCAGATTC AAACTATCAC TCAAGCCAAG 
GAGGTAATCC AAGGGGAAGT GAGGTGCCTA CAGCTGGAAC TGGATACTGA ACGGAGCCGG 
GCAGAACAGG AGCGGGATGC TGCAACCAAA CAGCTGGCCC AGGCTGAGCA AGAGGGGCAG 
ACCGCCCTGC AGCAGCAGAA GTCAGCCCAT GAGGAGGAAG TGAAGGGGCT CCAGGAAAAA 
TGGGAGAAAG AGCGTGCCTG GCACCAGCAG GAACTGGATA AGGCTCTGAA GAGCCTTGAG 
AGGGAGAAAA CGGAGCTGGA AATGAGGCTG AGGGAGCAGC AGGCAGAAAC AGAGGCCATG 
CGGACGCAGA GGGAAGAAGA ACGGGCTGAG GCTGAGAGCA CCCTGCGCCA GATGCAGCTC 
GAAACAGAGA AGGAGAGAAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGATGCCA GCCAACAACT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GAAGTTAAAG 
GAGCAGGAGA CCACTGGGAT GCTGCAGACC CAGCTCCGGG AAGCTCAGAA GGAGCTGGAA 
CAGGCAGCCC AGCAGCACAG AGACAGCCTT GCTGCCCTCC AAGAAGAAGG CCGTGCCCTG 
CTGCAGGATA AGATAGACCT GCACAAGCAG GTGGAGGACT TGAAGTCTCA GCTGGTTTCC 
AAGGAAGACT CCCAAAGGCT GGTAGACCAG GAAGTTCAGG AGAAGCTGAG AGAGGCCCAG 
GAATGTAGCC GAATTCAGAA GGAGCTGGAG AAAGAAAAGG CCAGCCTGAC TCTGTTGCTG 
GTGGAAAAGG AACAGAGACT CCTTGGTTTA CAAGAGGCCG ACTCCATTCG ACAACAAGAG 
CTGAGCTCCC TGCGCCAGGA CATGCAGGAG GCCCAGGCAG GGCAGAAAGA GCTCAGCGCC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GCAAAGGAGA CTGACTTTCT GGCCCAGGAA 
GCGCGGCTGC TGGAAGAGCT GGAGGCATCT CAAGTAACAG AGCAGCAGCT ACGAGCTTCC 
TTGTGGGCCC AGGAAGCCAA GGCCGCACAA CTACAGCTGC GACTGCGCAG CACAGAGAGC 
CAGCTGGAGG TGCTGCTGGC AGAGCAGCAG CCTGGGCACC AGGCCCAGGC CCAGCTGGCC 
AGCCTTTGTG CTGTCCTGCA GCAGGCCCTG GGGTCTGTTT GTGAGAGCAG GCCTGAGCTG 
AGTGGCGGGG GAGACTCTGC TCCCTCCCTC TGGGGCCCTG AGCCAGACCA GAATGGAGCT 
AGGACCCTTT TCAAGAGAGG GCCCCTCCTG ACAGCTCTCT CGGCTGAGGC GGTGGCCTCT 
GCCCTTCACA AGCTTAACCA AGACCTGTGG AAGACTCAGC AGGCCCGGGA TGATCTGAGG 
GATCAGGCCC AGAAGCTGGG ACAACGTCTT ACTGAAACAG AGGCCGAGAA GAGCCAGCTC 
CACACAGAGT TGCAGGCTCT GCAGAGGCAG CTCTCCCAGA ACCAGGAAGA GAGATCCAAG 
TGGGAAGGAA AACAGACCTC CTTAGAATCT GAGCTGGTGG AACTGCATGA AACTGTGACA 
TCCTTACAGA GTCGCCTACG GCAAGCAGAG CTGCAGGGAA TAGAAGCCCA GAATGAGCAA 
GAGTTACTGC AGGCAGCCAA GGAGAACCTG ACAGCCCAGG TGGAACACCT CCAAGCAGCT 
GTTGCAGAAG CCAGGGCTCA GGCAAGCGCT GCCGAGGTCC TGGAGGAAGA CCTCAAAACT 
GCACGCTCAG CCCTGAAACT GAAAAGCAAG GAGGTGGAGA GTGAGCGTGA GCGAGCCCAG 
GCCCTACAGG AGCAGGGCGA GCTGAAGGTG GCCCAAGGGA AAGCTTTGCA GGAGAATTTG 
GCTCGCCTGA CTCAGACCCT ATCTGAGAGA GAAGGGGAGG TGGAAACTTT GCAGGGAAAA 
ATCCAGGAGC TGGAGAAGCA ACGGGAAATG CAAAAGGCTG CTTTGGAGGT GCTGTCTTTG 
GACCTCAAAA AGAGGAACCA AGAGGTGGAT CTGCAGCAAG AACAGATTCA GGAGCTGGAG 
GAGTGCAGAT CGGTTTTAGA GCATCTCCCC ATGGCAGTCC AGGAGCGAGA GCAGAAGCTG 
ACTCTGCAGA GGGAGCAGAT CAAGGAGCTC GAGAAGGACC GAGAGACACA GAGGAACATC 
TTGGAGCATC AGCTTCTGGA ACTTGAGAAG AAGGCCCAAG TGATTGAGTC CCAGAAAGGA 
CAGATTCAGG ATCTGAAGAA GCAGCTGGTT ACTCTGGAAT GCCTGGCCCT GGAACTAGAG 
GAAAACCATC ACAAAATAGA GTGCCAGCAA AAGACAATTG AGGAGCTGGA GGGCCAGAGG 
GAAATGCAGA GGGCAGCTCT GACCCATCTG ACACTGGACC TGGAGGAAAG GAGCCAGGAG 
CTGCAGGTAC AGGGCAGCCA GATCCGGGAG CTAGAGAGTC ACAACAGCCT CTTGGCAAGA 
GAGCTCCAGG AGAAGGACCA GGAGATGACG TCCCAGCAAG ACCAGACAGA GGAACTGCAG 
AGGCAGAAGG AGCATTTGAC ACAGGACCTC AAGAGGCGGG AACAGGAGAT AGTGCTGCTG 
AAGGAGAGGA TTCAGGTCTT GGAAGATCAG AGAACCCTAC AGACCAAGAT CCTAGAGGAG 
GACCTGGAGC AGATCAAAAT GTCCTTGAGG GAGCGAGGCC GGGACCTGGC CTCGCAGAGG 
CAGCTGATGC AGGAGCGGGC AGAGGAAGGG AAGGGCCAGA GTAAAGCACA GCGCGGGAGC 
CTGGAGCATA TGAAGCTGAT CCTGCGTGAT AAGGAGAAGG AGGTGGAATG CCAGCAGGAG 
CATATCCAGG AACTGCAGGA GCACAAGGCC CAGCTGGAGC AGCAGCTCCA GGGCTTACAC 
AGGAAGATGG GGGAGACCAG CCTTCTCCTG ACCCAGCGGG AGCAGGAGAT AGTGGCTCTG 
CAGCGGCACC TGCAGGAAGC CAGGGAACAG GGAGAGCTGA AAGAGCAGTC ACTTCAAGGT 
CAGCTGGAAG AGGCCCAGAG GGCGCTGGCC CGAAGGGACC AGGAGCTCAA GGCCCTGCAG 
CAGCAGCAGG CCCAAGGCCA GGAGGAAAAT GTGATGGAAA AGGCGGGCTT ACTGCAGAGA 
GAGCTGGAGC AGGCCCACGT GACACTGAAG GAGCGCCAGG CGGAGCTTGA GGATTACAAG 
GAGCATGTGA GAAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCAGCGGGT ACAGGCCCTG 
GAGGAGGTGT TGAGTGACCT AAGGGCTGAA TCTCGGGAAC AAGAGAAGGC CCTGCTGGCC 
CTCCAGCAGC AGTGTGCCGA GCAGGCCCAG GAGCACGAGG CAGAGGCCAG GGCCCTGCAG 
GACAGCTGGC TGCAGGCAGA GGCTATGCTC AAGGAACGGG ACCAGGAACT GGAGGCCCTG 
CGGGCAGAGG GCCTGTCTTC CCAGCATCGG GAGGAGGCTG CCTGTGGCCA GGTGGAGGCT 
CTGCAGGAGG CCCTCAGCAA GGCTCAGGCT GACCTGCAGG AGAAAGAGCA GCATCTCCTT 
GGGCAGGTGG AACTGAGCCG CAGCCTAGAG GCCAGCACGG CCACGTTGCA GGCTGCCCTG 
GACTCCTGCC AGGCACAGTC CCGGCAGCTG GAGGAGGCCC TGAGGAAGCG GGAAGGTGAG 
GTCCAGGTCC GGGACCTCCG ATACCAGGAG GCCATGCAGC AGCTCCAGCA GGCACTTACC 
CAGAGGGATG AAGAGCTGAG ACAACAGAGG AAACAGGGGC AGCTCCTGGA GAAGTCTCTG 
GCCCAGAGGG GCCAAGAGGA TGCAACCCAA GAGAAGCAAG AAGCAAAGCA CGAGAGAGAA 
GAGGAAGAGA TAAGGAGCCT TCGTGAGAGT CTGCAGGAGC TACAGCTGAC TCTAGCACAA 
AAGGAAGAGG AGATCCTGGA GCTGAGGGAG GCCCAGCAGA GGAAGAGTCT GGAGGACTTG 
TCTCACAAAG GCTGCCCAGG GAAGGAGCCG GCTACAACAT TTGATTCCTT AGGGCCCAGG 
CTGCAGCGGG AACTGGAGCG ACTACAGGCA GCACTGAAGC AGACAGAAGC CAGGGAGATC 
GAGTGGAGGG AGAAGGCCCA GGATCTGGCG CTGTCCCTGG CCCAGAGCAA GGCCAGTGTC 
AGCGATCTGC AGGAGGTAGC CCTGTTCCTG CAAGCATCTG TTCTGGAGCG GGACTCAGAA 
CAGCAGAGGC TGCAGGATGA GTTGGAGCTC ACCAAACTCA CTCTGGAGAA AGAGCGACTC 
CACAGCCCAG GCCCGAGCAG CAGAGCAGAA CGGGGGCCTA GAGGAGAGCC AGGTGTACAA 
CTGGGAGAGG TCTCGGGAGT GAAGACTGAG CCTAGTCCTG AGATGGAGGA GAGGCAGCTC 
TGGGAACAGA GGCTTGAGCA CCTGCAGCGA GCGGTGGCTC TCTTGGAGGT TGACCGAAGC 
CAGCTGCAAC AACATAACGC CCAGCTGCGG ACCACCCTGG AGCAGGTGGA GCGAGAGCGG 
AGGAAACTGA AGAGGGGTTC CATGCGTGCA TTGCGGGCAG GGGACCTGGA GAGCGGTGAA 
GCCACGGCAC CATCACCCAT GCAACAGGAT GGAAGAGGAA GGCAGAAGGG CTGCTCAGAT 
GCCAAGCACA TGGTTGAGCT GCAAAAAGAG GTGGCCCTCC TGCGAGCCCA GCTGGCCTTG 
GAGCGGAAGC AGAAGCAGGA CTACATTGCT CGCTCAGTTC AGACGAGCCG TGAGCTGGCG 
GACCTGCACC ACGGCCTCTC TCACTCCCTT CTCGCTGTAG CCCAAGCCCC CGAGGCCACC 
GTCTTAGAGG CCGAGACCCG CAAGCTGGAC GAGTCTCTGA CTCAAAGCCT GACATCCCCC 
GGGCCAGCAC TGCTGTGCCC CAGCTCCAGT GCTGCTCAGA CTGCCTCCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020933920.1
CDS	91..6564
Translation	MWVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_021078261.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078261.1

ORF Insert Sequence:

ATGTGGGTGA CCGAGCTTTC TGCTCTGCTG ACCCAGTCTC AGAAGCAGAA TGAAGATTAT 
GAAAAGATGG TAAAGGCTCT GAGAGAGACA ATGAAGATCC TGGAGGCAAA TCATGCAGAG 
TTAATGGAAC ATGAGGCATC TCTTAGTAGG AATGCCCAAG AGGAGAGGCT GTCTTTACAG 
CAAGTGATCA AGGATATAAC CCAGGCCATG GTAGAAGAAG GGGACCATAT GGCCCAAGGC 
TCTGGTCACG AGCACTCCTT GGACTTGGAT CCTAGTGGCT TCTTCTCCCA GTTTGATTCC 
CAGCAGCCAG ACAAGGCCCT CACTCTGGTG CGTTCTGTGT TGACTCAGAG ACGCCAGGCT 
GTGCAGGACC TAAAGCAGCA GCTTTCAGGC TGTCAGGAAG CTGTGAGCTC CTTGCAGCAG 
CAGCATCATC AGTGGGAAGA AGAGGGTGAG GCCTTGAGAC AGCGGCTGCA GAAGCTCACC 
GGGGAGCGGG ACACCCTGGC AGGGCAGACT GTGGGCCTGC AGGGGGAGGT GGACTCTCTG 
AGCAGGGAGC GAGAGCTCCT GCAAAAGACC AGGGAGGAGC TGCAGCAGCA GCTGGAGGTG 
CTGGAGCAGG AGGCCTGGCG ACTACGGAGG ACAAACATGG AGCTTCAGCT GCAGGGCGAC 
TCTGCTCAGG GCGAGAAGGA GGAGCAGCAG GAGGAGCTGC ACCTGGCTGT CCGAGAACGG 
GAGCGCCTTC AGGAGATGCT GGCGAGCCTG GAAGCCAAAC AGTCAGAATC TCTCAGTGAA 
CTGATCACCC TTCGGGAAGC CCTGGAGTCT AGTCGCCTAG AAGGGGAGTT GCTGAGGCAA 
GAGCAAACAG AAGTGACTGC TGTGCTAGCA AAGGCAGAAC AGTCTATTGT GGAGCTGTCA 
AGTTCTGAGA ACAGCCTGAA GGCTGAGGTA GCTGATCTTC GGGCTGCAGC TGTCAAGCTC 
AGTGCCTTAA ATGAGGCTTT GGCCTTAGAT AAAGTTGGAC TGAACCAGCA GCTTCTCCAG 
TTAGAACAGC AGAACCAGTC TGTGTGCAGC AGGATGGAGG CAGCAGAGCA GGCAAGAAGC 
GCTTTGCAGG TGGGCCTGGC AGAGGTGGAG AGGAGCAGGG AAGCGCTGTG GGAAAAGAAC 
ACTTACCTGG AGGCTCAGCT GCAAAAAGCA GAGGCGGCCA GGGCTGAGCT GCAGGCAGAT 
CTCAGGGGCG TCCAGGAAGA GAAGGAAGAA ATTCAAGAGA AACTAAGCGA GGCACATCAC 
CAGCAGGAGG CAGTCTCAGT TCAGATGGAG CAGCTGCGTC AGGAGGCAAA GCGGCAGGAA 
GAGGTGCTCG CCCGGGAAGT CCAGGAGAAG GAGGCTCTGG TGCGGGAAAA GGCGGCTCTT 
GAAGTGCGGC TGCAGGCTGT GGAGCGGGAC CGGCAGGACC TCTCTGAACA ACTGCTGGGG 
CTCAGCTCGG CCAAGGAGCT TCTGGAGAAC AGTCTGTTTG AGGCCCAGCA ACAGAATTCT 
CTGATAGAAG TCACCAAGGG GCAGCTGGAG GTCCAGATTC AAACTATCAC TCAAGCCAAG 
GAGGTAATCC AAGGGGAAGT GAGGTGCCTA CAGCTGGAAC TGGATACTGA ACGGAGCCGG 
GCAGAACAGG AGCGGGATGC TGCAACCAAA CAGCTGGCCC AGGCTGAGCA AGAGGGGCAG 
ACCGCCCTGC AGCAGCAGAA GTCAGCCCAT GAGGAGGAAG TGAAGGGGCT CCAGGAAAAA 
TGGGAGAAAG AGCGTGCCTG GCACCAGCAG GAACTGGATA AGGCTCTGAA GAGCCTTGAG 
AGGGAGAAAA CGGAGCTGGA AATGAGGCTG AGGGAGCAGC AGGCAGAAAC AGAGGCCATG 
CGGACGCAGA GGGAAGAAGA ACGGGCTGAG GCTGAGAGCA CCCTGCGCCA GATGCAGCTC 
GAAACAGAGA AGGAGAGAAT GTCCCTCCTG GAGACACTGC TGCAGACCCA GAAGGAGCTG 
GCAGATGCCA GCCAACAACT GGAGCGGCTG AGGCAGGACA TGAAGGTTCA GAAGTTAAAG 
GAGCAGGAGA CCACTGGGAT GCTGCAGACC CAGCTCCGGG AAGCTCAGAA GGAGCTGGAA 
CAGGCAGCCC AGCAGCACAG AGACAGCCTT GCTGCCCTCC AAGAAGAAGG CCGTGCCCTG 
CTGCAGGATA AGATAGACCT GCACAAGCAG GTGGAGGACT TGAAGTCTCA GCTGGTTTCC 
AAGGAAGACT CCCAAAGGCT GGTAGACCAG GAAGTTCAGG AGAAGCTGAG AGAGGCCCAG 
GAATGTAGCC GAATTCAGAA GGAGCTGGAG AAAGAAAAGG CCAGCCTGAC TCTGTTGCTG 
GTGGAAAAGG AACAGAGACT CCTTGGTTTA CAAGAGGCCG ACTCCATTCG ACAACAAGAG 
CTGAGCTCCC TGCGCCAGGA CATGCAGGAG GCCCAGGCAG GGCAGAAAGA GCTCAGCGCC 
CAGGTGGAAT TACTGAAGCA GGAGGTGAAG GCAAAGGAGA CTGACTTTCT GGCCCAGGAA 
GCGCGGCTGC TGGAAGAGCT GGAGGCATCT CAAGTAACAG AGCAGCAGCT ACGAGCTTCC 
TTGTGGGCCC AGGAAGCCAA GGCCGCACAA CTACAGCTGC GACTGCGCAG CACAGAGAGC 
CAGCTGGAGG TGCTGCTGGC AGAGCAGCAG CCTGGGCACC AGGCCCAGGC CCAGCTGGCC 
AGCCTTTGTG CTGTCCTGCA GCAGGCCCTG GGGTCTGTTT GTGAGAGCAG GCCTGAGCTG 
AGTGGCGGGG GAGACTCTGC TCCCTCCCTC TGGGGCCCTG AGCCAGACCA GAATGGAGCT 
AGGACCCTTT TCAAGAGAGG GCCCCTCCTG ACAGCTCTCT CGGCTGAGGC GGTGGCCTCT 
GCCCTTCACA AGCTTAACCA AGACCTGTGG AAGACTCAGC AGGCCCGGGA TGATCTGAGG 
GATCAGGCCC AGAAGCTGGG ACAACGTCTT ACTGAAACAG AGGCCGAGAA GAGCCAGCTC 
CACACAGAGT TGCAGGCTCT GCAGAGGCAG CTCTCCCAGA ACCAGGAAGA GAGATCCAAG 
TGGGAAGGAA AACAGACCTC CTTAGAATCT GAGCTGGTGG AACTGCATGA AACTGTGACA 
TCCTTACAGA GTCGCCTACG GCAAGCAGAG CTGCAGGGAA TAGAAGCCCA GAATGAGCAA 
GAGTTACTGC AGGCAGCCAA GGAGAACCTG ACAGCCCAGG TGGAACACCT CCAAGCAGCT 
GTTGCAGAAG CCAGGGCTCA GGCAAGCGCT GCCGAGGTCC TGGAGGAAGA CCTCAAAACT 
GCACGCTCAG CCCTGAAACT GAAAAGCAAG GAGGTGGAGA GTGAGCGTGA GCGAGCCCAG 
GCCCTACAGG AGCAGGGCGA GCTGAAGGTG GCCCAAGGGA AAGCTTTGCA GGAGAATTTG 
GCTCGCCTGA CTCAGACCCT ATCTGAGAGA GAAGGGGAGG TGGAAACTTT GCAGGGAAAA 
ATCCAGGAGC TGGAGAAGCA ACGGGAAATG CAAAAGGCTG CTTTGGAGGT GCTGTCTTTG 
GACCTCAAAA AGAGGAACCA AGAGGTGGAT CTGCAGCAAG AACAGATTCA GGAGCTGGAG 
GAGTGCAGAT CGGTTTTAGA GCATCTCCCC ATGGCAGTCC AGGAGCGAGA GCAGAAGCTG 
ACTCTGCAGA GGGAGCAGAT CAAGGAGCTC GAGAAGGACC GAGAGACACA GAGGAACATC 
TTGGAGCATC AGCTTCTGGA ACTTGAGAAG AAGGCCCAAG TGATTGAGTC CCAGAAAGGA 
CAGATTCAGG ATCTGAAGAA GCAGCTGGTT ACTCTGGAAT GCCTGGCCCT GGAACTAGAG 
GAAAACCATC ACAAAATAGA GTGCCAGCAA AAGACAATTG AGGAGCTGGA GGGCCAGAGG 
GAAATGCAGA GGGCAGCTCT GACCCATCTG ACACTGGACC TGGAGGAAAG GAGCCAGGAG 
CTGCAGGTAC AGGGCAGCCA GATCCGGGAG CTAGAGAGTC ACAACAGCCT CTTGGCAAGA 
GAGCTCCAGG AGAAGGACCA GGAGATGACG TCCCAGCAAG ACCAGACAGA GGAACTGCAG 
AGGCAGAAGG AGCATTTGAC ACAGGACCTC AAGAGGCGGG AACAGGAGAT AGTGCTGCTG 
AAGGAGAGGA TTCAGGTCTT GGAAGATCAG AGAACCCTAC AGACCAAGAT CCTAGAGGAG 
GACCTGGAGC AGATCAAAAT GTCCTTGAGG GAGCGAGGCC GGGACCTGGC CTCGCAGAGG 
CAGCTGATGC AGGAGCGGGC AGAGGAAGGG AAGGGCCAGA GTAAAGCACA GCGCGGGAGC 
CTGGAGCATA TGAAGCTGAT CCTGCGTGAT AAGGAGAAGG AGGTGGAATG CCAGCAGGAG 
CATATCCAGG AACTGCAGGA GCACAAGGCC CAGCTGGAGC AGCAGCTCCA GGGCTTACAC 
AGGAAGATGG GGGAGACCAG CCTTCTCCTG ACCCAGCGGG AGCAGGAGAT AGTGGCTCTG 
CAGCGGCACC TGCAGGAAGC CAGGGAACAG GGAGAGCTGA AAGAGCAGTC ACTTCAAGGT 
CAGCTGGAAG AGGCCCAGAG GGCGCTGGCC CGAAGGGACC AGGAGCTCAA GGCCCTGCAG 
CAGCAGCAGG CCCAAGGCCA GGAGGAAAAT GTGATGGAAA AGGCGGGCTT ACTGCAGAGA 
GAGCTGGAGC AGGCCCACGT GACACTGAAG GAGCGCCAGG CGGAGCTTGA GGATTACAAG 
GAGCATGTGA GAAGGCTCCA GGAAGAGCTG GCAGTGGAGG GGCAGCGGGT ACAGGCCCTG 
GAGGAGGTGT TGAGTGACCT AAGGGCTGAA TCTCGGGAAC AAGAGAAGGC CCTGCTGGCC 
CTCCAGCAGC AGTGTGCCGA GCAGGCCCAG GAGCACGAGG CAGAGGCCAG GGCCCTGCAG 
GACAGCTGGC TGCAGGCAGA GGCTATGCTC AAGGAACGGG ACCAGGAACT GGAGGCCCTG 
CGGGCAGAGG GCCTGTCTTC CCAGCATCGG GAGGAGGCTG CCTGTGGCCA GGTGGAGGCT 
CTGCAGGAGG CCCTCAGCAA GGCTCAGGCT GACCTGCAGG AGAAAGAGCA GCATCTCCTT 
GGGCAGGTGG AACTGAGCCG CAGCCTAGAG GCCAGCACGG CCACGTTGCA GGCTGCCCTG 
GACTCCTGCC AGGCACAGTC CCGGCAGCTG GAGGAGGCCC TGAGGAAGCG GGAAGGTGAG 
GTCCAGGTCC GGGACCTCCG ATACCAGGAG GCCATGCAGC AGCTCCAGCA GGCACTTACC 
CAGAGGGATG AAGAGCTGAG ACAACAGAGG AAACAGGGGC AGCTCCTGGA GAAGTCTCTG 
GCCCAGAGGG GCCAAGAGGA TGCAACCCAA GAGAAGCAAG AAGCAAAGCA CGAGAGAGAA 
GAGGAAGAGA TAAGGAGCCT TCGTGAGAGT CTGCAGGAGC TACAGCTGAC TCTAGCACAA 
AAGGAAGAGG AGATCCTGGA GCTGAGGGAG GCCCAGCAGA GGAAGAGTCT GGAGGACTTG 
TCTCACAAAG GCTGCCCAGG GAAGGAGCCG GCTACAACAT TTGATTCCTT AGGGCCCAGG 
CTGCAGCGGG AACTGGAGCG ACTACAGGCA GCACTGAAGC AGACAGAAGC CAGGGAGATC 
GAGTGGAGGG AGAAGGCCCA GGATCTGGCG CTGTCCCTGG CCCAGAGCAA GGCCAGTGTC 
AGCGATCTGC AGGAGGTAGC CCTGTTCCTG CAAGCATCTG TTCTGGAGCG GGACTCAGAA 
CAGCAGAGGC TGCAGGATGA GTTGGAGCTC ACCAAACTCA CTCTGGAGAA AGAGCGACTC 
CACAGCCCAG GCCCGAGCAG CAGAGCAGAA CGGGGGCCTA GAGGAGAGCC AGGTGTACAA 
CTGGGAGAGG TCTCGGGAGT GAAGACTGAG CCTAGTCCTG AGATGGAGGA GAGGCAGCTC 
TGGGAACAGA GGCTTGAGCA CCTGCAGCGA GCGGTGGCTC TCTTGGAGGT TGACCGAAGC 
CAGCTGCAAC AACATAACGC CCAGCTGCGG ACCACCCTGG AGCAGGTGGA GCGAGAGCGG 
AGGAAACTGA AGAGGGGTTC CATGCGTGCA TTGCGGGCAG GGGACCTGGA GAGCGGTGAA 
GCCACGGCAC CATCACCCAT GCAACAGGAT GGAAGAGGAA GGCAGAAGGG CTGCTCAGAT 
GCCAAGCACA TGGTTGAGCT GCAAAAAGAG GTGGCCCTCC TGCGAGCCCA GCTGGCCTTG 
GAGCGGAAGC AGAAGCAGGA CTACATTGCT CGCTCAGTTC AGACGAGCCG TGAGCTGGCG 
GACCTGCACC ACGGCCTCTC TCACTCCCTT CTCGCTGTAG CCCAAGCCCC CGAGGCCACC 
GTCTTAGAGG CCGAGACCCG CAAGCTGGAC GAGTCTCTGA CTCAAAGCCT GACATCCCCC 
GGGCCAGCAC TGCTGTGCCC CAGCTCCAGT GCTGCTCAGA CTGCCTCCAG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_001929408.4 ) cDNA ORF clone, Sus scrofa(pig) -> XP_001929443.1 Sus scrofa centrosomal protein 250 (CEP250), transcript variant X1, mRNA.

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CloneID	OSe24918
Clone ID Related Accession (Same CDS sequence)	XM_001929408.4 , XM_013985306.2 , XM_001929408.3 , XM_013985307.1 , XM_013985308.1 , XM_013985306.1 , XM_013985307.2
Accession Version	XM_001929408.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010459.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_001929408.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001929443.1
CDS	1177..8499
Translation	METGRPRLNNMKPQPLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQATLVRKLQAKVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_001929408.4
Organism	Sus scrofa(pig)
Definition	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001929408.4

ORF Insert Sequence:

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_013985306.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013840760.1 Sus scrofa centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

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CloneID	OSe24918
Clone ID Related Accession (Same CDS sequence)	XM_001929408.4 , XM_013985306.2 , XM_001929408.3 , XM_013985307.1 , XM_013985308.1 , XM_013985306.1 , XM_013985307.2
Accession Version	XM_013985306.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010459.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013985306.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013840760.1
CDS	990..8312
Translation	METGRPRLNNMKPQPLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQATLVRKLQAKVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_013985306.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013985306.2

ORF Insert Sequence:

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_001929408.3 ) cDNA ORF clone, Sus scrofa(pig) -> XP_001929443.1 Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.

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CloneID	OSe24918
Clone ID Related Accession (Same CDS sequence)	XM_001929408.4 , XM_013985306.2 , XM_001929408.3 , XM_013985307.1 , XM_013985308.1 , XM_013985306.1 , XM_013985307.2
Accession Version	XM_001929408.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	centrosome-associated protein CEP250 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003612568.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 11, 2015 this sequence version replaced XM_001929408.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001929443.1
CDS	377..7699
Translation	METGRPRLNNMKPQPLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQATLVRKLQAKVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_001929408.3
Organism	Sus scrofa(Pig)
Definition	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001929408.3

ORF Insert Sequence:

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_013985307.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013840761.1 Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.

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CloneID	OSe24918
Clone ID Related Accession (Same CDS sequence)	XM_001929408.4 , XM_013985306.2 , XM_001929408.3 , XM_013985307.1 , XM_013985308.1 , XM_013985306.1 , XM_013985307.2
Accession Version	XM_013985307.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	centrosome-associated protein CEP250 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003612568.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013840761.1
CDS	268..7590
Translation	METGRPRLNNMKPQPLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQATLVRKLQAKVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_013985307.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013985307.1

ORF Insert Sequence:

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_013985308.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013840762.1 Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X4, mRNA.

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CloneID	OSe24918
Clone ID Related Accession (Same CDS sequence)	XM_001929408.4 , XM_013985306.2 , XM_001929408.3 , XM_013985307.1 , XM_013985308.1 , XM_013985306.1 , XM_013985307.2
Accession Version	XM_013985308.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	centrosome-associated protein CEP250 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003612568.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013840762.1
CDS	1..7323
Translation	METGRPRLNNMKPQPLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQATLVRKLQAKVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_013985308.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013985308.1

ORF Insert Sequence:

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_013985306.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013840760.1 Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.

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CloneID	OSe24918
Clone ID Related Accession (Same CDS sequence)	XM_001929408.4 , XM_013985306.2 , XM_001929408.3 , XM_013985307.1 , XM_013985308.1 , XM_013985306.1 , XM_013985307.2
Accession Version	XM_013985306.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	centrosome-associated protein CEP250 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003612568.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013840760.1
CDS	192..7514
Translation	METGRPRLNNMKPQPLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQATLVRKLQAKVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_013985306.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013985306.1

ORF Insert Sequence:

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_013985307.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013840761.1 Sus scrofa centrosomal protein 250 (CEP250), transcript variant X3, mRNA.

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CloneID	OSe24918
Clone ID Related Accession (Same CDS sequence)	XM_001929408.4 , XM_013985306.2 , XM_001929408.3 , XM_013985307.1 , XM_013985308.1 , XM_013985306.1 , XM_013985307.2
Accession Version	XM_013985307.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	centrosome-associated protein CEP250 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010459.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013985307.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013840761.1
CDS	1068..8390
Translation	METGRPRLNNMKPQPLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQATLVRKLQAKVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_013985307.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013985307.2

ORF Insert Sequence:

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATG GAAGAGGAAG GCAGAAGGGC 
TGCTCAGATG CCAAGCACAT GGTTGAGCTG CAAAAAGAGG TGGCCCTCCT GCGAGCCCAG 
CTGGCCTTGG AGCGGAAGCA GAAGCAGGAC TACATTGCTC GCTCAGTTCA GACGAGCCGT 
GAGCTGGCGG ACCTGCACCA CGGCCTCTCT CACTCCCTTC TCGCTGTAGC CCAAGCCCCC 
GAGGCCACCG TCTTAGAGGC CGAGACCCGC AAGCTGGACG AGTCTCTGAC TCAAAGCCTG 
ACATCCCCCG GGCCAGCACT GCTGTGCCCC AGCTCCAGTG CTGCTCAGAC TGCCTCCAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_013985309.2 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013840763.1 Sus scrofa centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

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CloneID	OSe44857
Clone ID Related Accession (Same CDS sequence)	XM_013985309.2 , XM_013985309.1
Accession Version	XM_013985309.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	centrosome-associated protein CEP250 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010459.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 13, 2017 this sequence version replaced XM_013985309.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACCT TCACAAGGGT GTTGCAGTAC CGGAGCTGGA GCCAAGAGCT GGAGAAGCGG 
CTGGAAGCCA CTGGGGCACC AATCCCTCAG AGGTGGGAAA ATGTGGAGGA GCCAAACCTG 
GAACAGCTGC TAATCCGATT GGAGGAGGAG CAACAGAGGT GTGAGAGTCT AGCAGAGGTG 
AACACCCAGC TTCGGCTGCA CATGGAAAAA GCTGACGTGG TGAATAAAGC GCTTCGGGAA 
GATGTGGAAA AATTGACAGT AGACTGGAGC CAGGCCCGGG ATGAGCTAAT GAGGAAGGAG 
AGCCAGTGGC AGATGGAGCA GGAGTTCTTC AAGGGCTACC TGAAAGGGGA GCATGGTCGC 
CTTCTCAGTC TATGGCGGGA GGTGGTGACC TTCCGACGTC ACTTTCTGGA AATGAAGTCA 
GCCACTGACA GAGACCTGAT GGAGCTCAAG GCTGAGCACG TGAAGCTCTC TGGGTCCCTG 
TTGACCTGTT GTCTGCGCTT GACTGTGGGA GCACCACAGG CCCGGGAATC CACTGGATCT 
GGGAGGCTGA ATGGGAGTGA ACCATCCCAG CTGATGCTGC TACTGTCTAA GACCCAGGAG 
CTGGAGAAGG AAGCCCATAA AAGGAGCCAG GAGTTAATAC AGCTTAAGAG TCAAGGGGAT 
CTGGAGAAGG CTGAGCTTCA GGATCGGGTG ACCGAGCTTT CTGCTCTGCT GACCCAGTCT 
CAGAAGCAGA ATGAAGATTA TGAAAAGATG GTAAAGGCTC TGAGAGAGAC AATGAAGATC 
CTGGAGGCAA ATCATGCAGA GTTAATGGAA CATGAGGCAT CTCTTAGTAG GAATGCCCAA 
GAGGAGAGGC TGTCTTTACA GCAAGTGATC AAGGATATAA CCCAGGCCAT GGTAGAAGAA 
GGGGACCATA TGGCCCAAGG CTCTGGTCAC GAGCACTCCT TGGACTTGGA TCCTAGTGGC 
TTCTTCTCCC AGTTTGATTC CCAGCAGCCA GACAAGGCCC TCACTCTGGT GCGTTCTGTG 
TTGACTCAGA GACGCCAGGC TGTGCAGGAC CTAAAGCAGC AGCTTTCAGG CTGTCAGGAA 
GCTGTGAGCT CCTTGCAGCA GCAGCATCAT CAGTGGGAAG AAGAGGGTGA GGCCTTGAGA 
CAGCGGCTGC AGAAGCTCAC CGGGGAGCGG GACACCCTGG CAGGGCAGAC TGTGGGCCTG 
CAGGGGGAGG TGGACTCTCT GAGCAGGGAG CGAGAGCTCC TGCAAAAGAC CAGGGAGGAG 
CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG GAGGCCTGGC GACTACGGAG GACAAACATG 
GAGCTTCAGC TGCAGGGCGA CTCTGCTCAG GGCGAGAAGG AGGAGCAGCA GGAGGAGCTG 
CACCTGGCTG TCCGAGAACG GGAGCGCCTT CAGGAGATGC TGGCGAGCCT GGAAGCCAAA 
CAGTCAGAAT CTCTCAGTGA ACTGATCACC CTTCGGGAAG CCCTGGAGTC TAGTCGCCTA 
GAAGGGGAGT TGCTGAGGCA AGAGCAAACA GAAGTGACTG CTGTGCTAGC AAAGGCAGAA 
CAGTCTATTG TGGAGCTGTC AAGTTCTGAG AACAGCCTGA AGGCTGAGGT AGCTGATCTT 
CGGGCTGCAG CTGTCAAGCT CAGTGCCTTA AATGAGGCTT TGGCCTTAGA TAAAGTTGGA 
CTGAACCAGC AGCTTCTCCA GTTAGAACAG CAGAACCAGT CTGTGTGCAG CAGGATGGAG 
GCAGCAGAGC AGGCAAGAAG CGCTTTGCAG GTGGGCCTGG CAGAGGTGGA GAGGAGCAGG 
GAAGCGCTGT GGGAAAAGAA CACTTACCTG GAGGCTCAGC TGCAAAAAGC AGAGGCGGCC 
AGGGCTGAGC TGCAGGCAGA TCTCAGGGGC GTCCAGGAAG AGAAGGAAGA AATTCAAGAG 
AAACTAAGCG AGGCACATCA CCAGCAGGAG GCAGTCTCAG TTCAGATGGA GCAGCTGCGT 
CAGGAGGCAA AGCGGCAGGA AGAGGTGCTC GCCCGGGAAG TCCAGGAGAA GGAGGCTCTG 
GTGCGGGAAA AGGCGGCTCT TGAAGTGCGG CTGCAGGCTG TGGAGCGGGA CCGGCAGGAC 
CTCTCTGAAC AACTGCTGGG GCTCAGCTCG GCCAAGGAGC TTCTGGAGAA CAGTCTGTTT 
GAGGCCCAGC AACAGAATTC TCTGATAGAA GTCACCAAGG GGCAGCTGGA GGTCCAGATT 
CAAACTATCA CTCAAGCCAA GGAGGTAATC CAAGGGGAAG TGAGGTGCCT ACAGCTGGAA 
CTGGATACTG AACGGAGCCG GGCAGAACAG GAGCGGGATG CTGCAACCAA ACAGCTGGCC 
CAGGCTGAGC AAGAGGGGCA GACCGCCCTG CAGCAGCAGA AGTCAGCCCA TGAGGAGGAA 
GTGAAGGGGC TCCAGGAAAA ATGGGAGAAA GAGCGTGCCT GGCACCAGCA GGAACTGGAT 
AAGGCTCTGA AGAGCCTTGA GAGGGAGAAA ACGGAGCTGG AAATGAGGCT GAGGGAGCAG 
CAGGCAGAAA CAGAGGCCAT GCGGACGCAG AGGGAAGAAG AACGGGCTGA GGCTGAGAGC 
ACCCTGCGCC AGATGCAGCT CGAAACAGAG AAGGAGAGAA TGTCCCTCCT GGAGACACTG 
CTGCAGACCC AGAAGGAGCT GGCAGATGCC AGCCAACAAC TGGAGCGGCT GAGGCAGGAC 
ATGAAGGTTC AGAAGTTAAA GGAGCAGGAG ACCACTGGGA TGCTGCAGAC CCAGCTCCGG 
GAAGCTCAGA AGGAGCTGGA ACAGGCAGCC CAGCAGCACA GAGACAGCCT TGCTGCCCTC 
CAAGAAGAAG GCCGTGCCCT GCTGCAGGAT AAGATAGACC TGCACAAGCA GGTGGAGGAC 
TTGAAGTCTC AGCTGGTTTC CAAGGAAGAC TCCCAAAGGC TGGTAGACCA GGAAGTTCAG 
GAGAAGCTGA GAGAGGCCCA GGAATGTAGC CGAATTCAGA AGGAGCTGGA GAAAGAAAAG 
GCCAGCCTGA CTCTGTTGCT GGTGGAAAAG GAACAGAGAC TCCTTGGTTT ACAAGAGGCC 
GACTCCATTC GACAACAAGA GCTGAGCTCC CTGCGCCAGG ACATGCAGGA GGCCCAGGCA 
GGGCAGAAAG AGCTCAGCGC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGCAAAGGAG 
ACTGACTTTC TGGCCCAGGA AGCGCGGCTG CTGGAAGAGC TGGAGGCATC TCAAGTAACA 
GAGCAGCAGC TACGAGCTTC CTTGTGGGCC CAGGAAGCCA AGGCCGCACA ACTACAGCTG 
CGACTGCGCA GCACAGAGAG CCAGCTGGAG GTGCTGCTGG CAGAGCAGCA GCCTGGGCAC 
CAGGCCCAGG CCCAGCTGGC CAGCCTTTGT GCTGTCCTGC AGCAGGCCCT GGGGTCTGTT 
TGTGAGAGCA GGCCTGAGCT GAGTGGCGGG GGAGACTCTG CTCCCTCCCT CTGGGGCCCT 
GAGCCAGACC AGAATGGAGC TAGGACCCTT TTCAAGAGAG GGCCCCTCCT GACAGCTCTC 
TCGGCTGAGG CGGTGGCCTC TGCCCTTCAC AAGCTTAACC AAGACCTGTG GAAGACTCAG 
CAGGCCCGGG ATGATCTGAG GGATCAGGCC CAGAAGCTGG GACAACGTCT TACTGAAACA 
GAGGCCGAGA AGAGCCAGCT CCACACAGAG TTGCAGGCTC TGCAGAGGCA GCTCTCCCAG 
AACCAGGAAG AGAGATCCAA GTGGGAAGGA AAACAGACCT CCTTAGAATC TGAGCTGGTG 
GAACTGCATG AAACTGTGAC ATCCTTACAG AGTCGCCTAC GGCAAGCAGA GCTGCAGGGA 
ATAGAAGCCC AGAATGAGCA AGAGTTACTG CAGGCAGCCA AGGAGAACCT GACAGCCCAG 
GTGGAACACC TCCAAGCAGC TGTTGCAGAA GCCAGGGCTC AGGCAAGCGC TGCCGAGGTC 
CTGGAGGAAG ACCTCAAAAC TGCACGCTCA GCCCTGAAAC TGAAAAGCAA GGAGGTGGAG 
AGTGAGCGTG AGCGAGCCCA GGCCCTACAG GAGCAGGGCG AGCTGAAGGT GGCCCAAGGG 
AAAGCTTTGC AGGAGAATTT GGCTCGCCTG ACTCAGACCC TATCTGAGAG AGAAGGGGAG 
GTGGAAACTT TGCAGGGAAA AATCCAGGAG CTGGAGAAGC AACGGGAAAT GCAAAAGGCT 
GCTTTGGAGG TGCTGTCTTT GGACCTCAAA AAGAGGAACC AAGAGGTGGA TCTGCAGCAA 
GAACAGATTC AGGAGCTGGA GGAGTGCAGA TCGGTTTTAG AGCATCTCCC CATGGCAGTC 
CAGGAGCGAG AGCAGAAGCT GACTCTGCAG AGGGAGCAGA TCAAGGAGCT CGAGAAGGAC 
CGAGAGACAC AGAGGAACAT CTTGGAGCAT CAGCTTCTGG AACTTGAGAA GAAGGCCCAA 
GTGATTGAGT CCCAGAAAGG ACAGATTCAG GATCTGAAGA AGCAGCTGGT TACTCTGGAA 
TGCCTGGCCC TGGAACTAGA GGAAAACCAT CACAAAATAG AGTGCCAGCA AAAGACAATT 
GAGGAGCTGG AGGGCCAGAG GGAAATGCAG AGGGCAGCTC TGACCCATCT GACACTGGAC 
CTGGAGGAAA GGAGCCAGGA GCTGCAGGTA CAGGGCAGCC AGATCCGGGA GCTAGAGAGT 
CACAACAGCC TCTTGGCAAG AGAGCTCCAG GAGAAGGACC AGGAGATGAC GTCCCAGCAA 
GACCAGACAG AGGAACTGCA GAGGCAGAAG GAGCATTTGA CACAGGACCT CAAGAGGCGG 
GAACAGGAGA TAGTGCTGCT GAAGGAGAGG ATTCAGGTCT TGGAAGATCA GAGAACCCTA 
CAGACCAAGA TCCTAGAGGA GGACCTGGAG CAGATCAAAA TGTCCTTGAG GGAGCGAGGC 
CGGGACCTGG CCTCGCAGAG GCAGCTGATG CAGGAGCGGG CAGAGGAAGG GAAGGGCCAG 
AGTAAAGCAC AGCGCGGGAG CCTGGAGCAT ATGAAGCTGA TCCTGCGTGA TAAGGAGAAG 
GAGGTGGAAT GCCAGCAGGA GCATATCCAG GAACTGCAGG AGCACAAGGC CCAGCTGGAG 
CAGCAGCTCC AGGGCTTACA CAGGAAGATG GGGGAGACCA GCCTTCTCCT GACCCAGCGG 
GAGCAGGAGA TAGTGGCTCT GCAGCGGCAC CTGCAGGAAG CCAGGGAACA GGGAGAGCTG 
AAAGAGCAGT CACTTCAAGG TCAGCTGGAA GAGGCCCAGA GGGCGCTGGC CCGAAGGGAC 
CAGGAGCTCA AGGCCCTGCA GCAGCAGCAG GCCCAAGGCC AGGAGGAAAA TGTGATGGAA 
AAGGCGGGCT TACTGCAGAG AGAGCTGGAG CAGGCCCACG TGACACTGAA GGAGCGCCAG 
GCGGAGCTTG AGGATTACAA GGAGCATGTG AGAAGGCTCC AGGAAGAGCT GGCAGTGGAG 
GGGCAGCGGG TACAGGCCCT GGAGGAGGTG TTGAGTGACC TAAGGGCTGA ATCTCGGGAA 
CAAGAGAAGG CCCTGCTGGC CCTCCAGCAG CAGTGTGCCG AGCAGGCCCA GGAGCACGAG 
GCAGAGGCCA GGGCCCTGCA GGACAGCTGG CTGCAGGCAG AGGCTATGCT CAAGGAACGG 
GACCAGGAAC TGGAGGCCCT GCGGGCAGAG GGCCTGTCTT CCCAGCATCG GGAGGAGGCT 
GCCTGTGGCC AGGTGGAGGC TCTGCAGGAG GCCCTCAGCA AGGCTCAGGC TGACCTGCAG 
GAGAAAGAGC AGCATCTCCT TGGGCAGGTG GAACTGAGCC GCAGCCTAGA GGCCAGCACG 
GCCACGTTGC AGGCTGCCCT GGACTCCTGC CAGGCACAGT CCCGGCAGCT GGAGGAGGCC 
CTGAGGAAGC GGGAAGGTGA GGTCCAGGTC CGGGACCTCC GATACCAGGA GGCCATGCAG 
CAGCTCCAGC AGGCACTTAC CCAGAGGGAT GAAGAGCTGA GACAACAGAG GAAACAGGGG 
CAGCTCCTGG AGAAGTCTCT GGCCCAGAGG GGCCAAGAGG ATGCAACCCA AGAGAAGCAA 
GAAGCAAAGC ACGAGAGAGA AGAGGAAGAG ATAAGGAGCC TTCGTGAGAG TCTGCAGGAG 
CTACAGCTGA CTCTAGCACA AAAGGAAGAG GAGATCCTGG AGCTGAGGGA GGCCCAGCAG 
AGGAAGAGTC TGGAGGACTT GTCTCACAAA GGCTGCCCAG GGAAGGAGCC GGCTACAACA 
TTTGATTCCT TAGGGCCCAG GCTGCAGCGG GAACTGGAGC GACTACAGGC AGCACTGAAG 
CAGACAGAAG CCAGGGAGAT CGAGTGGAGG GAGAAGGCCC AGGATCTGGC GCTGTCCCTG 
GCCCAGAGCA AGGCCAGTGT CAGCGATCTG CAGGAGGTAG CCCTGTTCCT GCAAGCATCT 
GTTCTGGAGC GGGACTCAGA ACAGCAGAGG CTGCAGGATG AGTTGGAGCT CACCAAACTC 
ACTCTGGAGA AAGAGCGACT CCACAGCCCA GGCCCGAGCA GCAGAGCAGA ACGGGGGCCT 
AGAGGAGAGC CAGGTGTACA ACTGGGAGAG GTCTCGGGAG TGAAGACTGA GCCTAGTCCT 
GAGATGGAGG AGAGGCAGCT CTGGGAACAG AGGCTTGAGC ACCTGCAGCG AGCGGTGGCT 
CTCTTGGAGG TTGACCGAAG CCAGCTGCAA CAACATAACG CCCAGCTGCG GACCACCCTG 
GAGCAGGTGG AGCGAGAGCG GAGGAAACTG AAGAGGGGTT CCATGCGTGC ATTGCGGGCA 
GGGGACCTGG AGAGCGGTGA AGCCACGGCA CCATCACCCA TGCAACAGGA TGGAAGAGGA 
AGGCAGAAGG GCTGCTCAGA TGCCAAGCAC ATGGTTGAGC TGCAAAAAGA GGTGGCCCTC 
CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG CAGAAGCAGG ACTACATTGC TCGCTCAGTT 
CAGACGAGCC GTGAGCTGGC GGACCTGCAC CACGGCCTCT CTCACTCCCT TCTCGCTGTA 
GCCCAAGCCC CCGAGGCCAC CGTCTTAGAG GCCGAGACCC GCAAGCTGGA CGAGTCTCTG 
ACTCAAAGCC TGACATCCCC CGGGCCAGCA CTGCTGTGCC CCAGCTCCAG TGCTGCTCAG 
ACTGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013840763.1
CDS	111..7265
Translation	MMTFTRVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_013985309.2
Organism	Sus scrofa(pig)
Definition	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013985309.2

ORF Insert Sequence:

ATGATGACCT TCACAAGGGT GTTGCAGTAC CGGAGCTGGA GCCAAGAGCT GGAGAAGCGG 
CTGGAAGCCA CTGGGGCACC AATCCCTCAG AGGTGGGAAA ATGTGGAGGA GCCAAACCTG 
GAACAGCTGC TAATCCGATT GGAGGAGGAG CAACAGAGGT GTGAGAGTCT AGCAGAGGTG 
AACACCCAGC TTCGGCTGCA CATGGAAAAA GCTGACGTGG TGAATAAAGC GCTTCGGGAA 
GATGTGGAAA AATTGACAGT AGACTGGAGC CAGGCCCGGG ATGAGCTAAT GAGGAAGGAG 
AGCCAGTGGC AGATGGAGCA GGAGTTCTTC AAGGGCTACC TGAAAGGGGA GCATGGTCGC 
CTTCTCAGTC TATGGCGGGA GGTGGTGACC TTCCGACGTC ACTTTCTGGA AATGAAGTCA 
GCCACTGACA GAGACCTGAT GGAGCTCAAG GCTGAGCACG TGAAGCTCTC TGGGTCCCTG 
TTGACCTGTT GTCTGCGCTT GACTGTGGGA GCACCACAGG CCCGGGAATC CACTGGATCT 
GGGAGGCTGA ATGGGAGTGA ACCATCCCAG CTGATGCTGC TACTGTCTAA GACCCAGGAG 
CTGGAGAAGG AAGCCCATAA AAGGAGCCAG GAGTTAATAC AGCTTAAGAG TCAAGGGGAT 
CTGGAGAAGG CTGAGCTTCA GGATCGGGTG ACCGAGCTTT CTGCTCTGCT GACCCAGTCT 
CAGAAGCAGA ATGAAGATTA TGAAAAGATG GTAAAGGCTC TGAGAGAGAC AATGAAGATC 
CTGGAGGCAA ATCATGCAGA GTTAATGGAA CATGAGGCAT CTCTTAGTAG GAATGCCCAA 
GAGGAGAGGC TGTCTTTACA GCAAGTGATC AAGGATATAA CCCAGGCCAT GGTAGAAGAA 
GGGGACCATA TGGCCCAAGG CTCTGGTCAC GAGCACTCCT TGGACTTGGA TCCTAGTGGC 
TTCTTCTCCC AGTTTGATTC CCAGCAGCCA GACAAGGCCC TCACTCTGGT GCGTTCTGTG 
TTGACTCAGA GACGCCAGGC TGTGCAGGAC CTAAAGCAGC AGCTTTCAGG CTGTCAGGAA 
GCTGTGAGCT CCTTGCAGCA GCAGCATCAT CAGTGGGAAG AAGAGGGTGA GGCCTTGAGA 
CAGCGGCTGC AGAAGCTCAC CGGGGAGCGG GACACCCTGG CAGGGCAGAC TGTGGGCCTG 
CAGGGGGAGG TGGACTCTCT GAGCAGGGAG CGAGAGCTCC TGCAAAAGAC CAGGGAGGAG 
CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG GAGGCCTGGC GACTACGGAG GACAAACATG 
GAGCTTCAGC TGCAGGGCGA CTCTGCTCAG GGCGAGAAGG AGGAGCAGCA GGAGGAGCTG 
CACCTGGCTG TCCGAGAACG GGAGCGCCTT CAGGAGATGC TGGCGAGCCT GGAAGCCAAA 
CAGTCAGAAT CTCTCAGTGA ACTGATCACC CTTCGGGAAG CCCTGGAGTC TAGTCGCCTA 
GAAGGGGAGT TGCTGAGGCA AGAGCAAACA GAAGTGACTG CTGTGCTAGC AAAGGCAGAA 
CAGTCTATTG TGGAGCTGTC AAGTTCTGAG AACAGCCTGA AGGCTGAGGT AGCTGATCTT 
CGGGCTGCAG CTGTCAAGCT CAGTGCCTTA AATGAGGCTT TGGCCTTAGA TAAAGTTGGA 
CTGAACCAGC AGCTTCTCCA GTTAGAACAG CAGAACCAGT CTGTGTGCAG CAGGATGGAG 
GCAGCAGAGC AGGCAAGAAG CGCTTTGCAG GTGGGCCTGG CAGAGGTGGA GAGGAGCAGG 
GAAGCGCTGT GGGAAAAGAA CACTTACCTG GAGGCTCAGC TGCAAAAAGC AGAGGCGGCC 
AGGGCTGAGC TGCAGGCAGA TCTCAGGGGC GTCCAGGAAG AGAAGGAAGA AATTCAAGAG 
AAACTAAGCG AGGCACATCA CCAGCAGGAG GCAGTCTCAG TTCAGATGGA GCAGCTGCGT 
CAGGAGGCAA AGCGGCAGGA AGAGGTGCTC GCCCGGGAAG TCCAGGAGAA GGAGGCTCTG 
GTGCGGGAAA AGGCGGCTCT TGAAGTGCGG CTGCAGGCTG TGGAGCGGGA CCGGCAGGAC 
CTCTCTGAAC AACTGCTGGG GCTCAGCTCG GCCAAGGAGC TTCTGGAGAA CAGTCTGTTT 
GAGGCCCAGC AACAGAATTC TCTGATAGAA GTCACCAAGG GGCAGCTGGA GGTCCAGATT 
CAAACTATCA CTCAAGCCAA GGAGGTAATC CAAGGGGAAG TGAGGTGCCT ACAGCTGGAA 
CTGGATACTG AACGGAGCCG GGCAGAACAG GAGCGGGATG CTGCAACCAA ACAGCTGGCC 
CAGGCTGAGC AAGAGGGGCA GACCGCCCTG CAGCAGCAGA AGTCAGCCCA TGAGGAGGAA 
GTGAAGGGGC TCCAGGAAAA ATGGGAGAAA GAGCGTGCCT GGCACCAGCA GGAACTGGAT 
AAGGCTCTGA AGAGCCTTGA GAGGGAGAAA ACGGAGCTGG AAATGAGGCT GAGGGAGCAG 
CAGGCAGAAA CAGAGGCCAT GCGGACGCAG AGGGAAGAAG AACGGGCTGA GGCTGAGAGC 
ACCCTGCGCC AGATGCAGCT CGAAACAGAG AAGGAGAGAA TGTCCCTCCT GGAGACACTG 
CTGCAGACCC AGAAGGAGCT GGCAGATGCC AGCCAACAAC TGGAGCGGCT GAGGCAGGAC 
ATGAAGGTTC AGAAGTTAAA GGAGCAGGAG ACCACTGGGA TGCTGCAGAC CCAGCTCCGG 
GAAGCTCAGA AGGAGCTGGA ACAGGCAGCC CAGCAGCACA GAGACAGCCT TGCTGCCCTC 
CAAGAAGAAG GCCGTGCCCT GCTGCAGGAT AAGATAGACC TGCACAAGCA GGTGGAGGAC 
TTGAAGTCTC AGCTGGTTTC CAAGGAAGAC TCCCAAAGGC TGGTAGACCA GGAAGTTCAG 
GAGAAGCTGA GAGAGGCCCA GGAATGTAGC CGAATTCAGA AGGAGCTGGA GAAAGAAAAG 
GCCAGCCTGA CTCTGTTGCT GGTGGAAAAG GAACAGAGAC TCCTTGGTTT ACAAGAGGCC 
GACTCCATTC GACAACAAGA GCTGAGCTCC CTGCGCCAGG ACATGCAGGA GGCCCAGGCA 
GGGCAGAAAG AGCTCAGCGC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGCAAAGGAG 
ACTGACTTTC TGGCCCAGGA AGCGCGGCTG CTGGAAGAGC TGGAGGCATC TCAAGTAACA 
GAGCAGCAGC TACGAGCTTC CTTGTGGGCC CAGGAAGCCA AGGCCGCACA ACTACAGCTG 
CGACTGCGCA GCACAGAGAG CCAGCTGGAG GTGCTGCTGG CAGAGCAGCA GCCTGGGCAC 
CAGGCCCAGG CCCAGCTGGC CAGCCTTTGT GCTGTCCTGC AGCAGGCCCT GGGGTCTGTT 
TGTGAGAGCA GGCCTGAGCT GAGTGGCGGG GGAGACTCTG CTCCCTCCCT CTGGGGCCCT 
GAGCCAGACC AGAATGGAGC TAGGACCCTT TTCAAGAGAG GGCCCCTCCT GACAGCTCTC 
TCGGCTGAGG CGGTGGCCTC TGCCCTTCAC AAGCTTAACC AAGACCTGTG GAAGACTCAG 
CAGGCCCGGG ATGATCTGAG GGATCAGGCC CAGAAGCTGG GACAACGTCT TACTGAAACA 
GAGGCCGAGA AGAGCCAGCT CCACACAGAG TTGCAGGCTC TGCAGAGGCA GCTCTCCCAG 
AACCAGGAAG AGAGATCCAA GTGGGAAGGA AAACAGACCT CCTTAGAATC TGAGCTGGTG 
GAACTGCATG AAACTGTGAC ATCCTTACAG AGTCGCCTAC GGCAAGCAGA GCTGCAGGGA 
ATAGAAGCCC AGAATGAGCA AGAGTTACTG CAGGCAGCCA AGGAGAACCT GACAGCCCAG 
GTGGAACACC TCCAAGCAGC TGTTGCAGAA GCCAGGGCTC AGGCAAGCGC TGCCGAGGTC 
CTGGAGGAAG ACCTCAAAAC TGCACGCTCA GCCCTGAAAC TGAAAAGCAA GGAGGTGGAG 
AGTGAGCGTG AGCGAGCCCA GGCCCTACAG GAGCAGGGCG AGCTGAAGGT GGCCCAAGGG 
AAAGCTTTGC AGGAGAATTT GGCTCGCCTG ACTCAGACCC TATCTGAGAG AGAAGGGGAG 
GTGGAAACTT TGCAGGGAAA AATCCAGGAG CTGGAGAAGC AACGGGAAAT GCAAAAGGCT 
GCTTTGGAGG TGCTGTCTTT GGACCTCAAA AAGAGGAACC AAGAGGTGGA TCTGCAGCAA 
GAACAGATTC AGGAGCTGGA GGAGTGCAGA TCGGTTTTAG AGCATCTCCC CATGGCAGTC 
CAGGAGCGAG AGCAGAAGCT GACTCTGCAG AGGGAGCAGA TCAAGGAGCT CGAGAAGGAC 
CGAGAGACAC AGAGGAACAT CTTGGAGCAT CAGCTTCTGG AACTTGAGAA GAAGGCCCAA 
GTGATTGAGT CCCAGAAAGG ACAGATTCAG GATCTGAAGA AGCAGCTGGT TACTCTGGAA 
TGCCTGGCCC TGGAACTAGA GGAAAACCAT CACAAAATAG AGTGCCAGCA AAAGACAATT 
GAGGAGCTGG AGGGCCAGAG GGAAATGCAG AGGGCAGCTC TGACCCATCT GACACTGGAC 
CTGGAGGAAA GGAGCCAGGA GCTGCAGGTA CAGGGCAGCC AGATCCGGGA GCTAGAGAGT 
CACAACAGCC TCTTGGCAAG AGAGCTCCAG GAGAAGGACC AGGAGATGAC GTCCCAGCAA 
GACCAGACAG AGGAACTGCA GAGGCAGAAG GAGCATTTGA CACAGGACCT CAAGAGGCGG 
GAACAGGAGA TAGTGCTGCT GAAGGAGAGG ATTCAGGTCT TGGAAGATCA GAGAACCCTA 
CAGACCAAGA TCCTAGAGGA GGACCTGGAG CAGATCAAAA TGTCCTTGAG GGAGCGAGGC 
CGGGACCTGG CCTCGCAGAG GCAGCTGATG CAGGAGCGGG CAGAGGAAGG GAAGGGCCAG 
AGTAAAGCAC AGCGCGGGAG CCTGGAGCAT ATGAAGCTGA TCCTGCGTGA TAAGGAGAAG 
GAGGTGGAAT GCCAGCAGGA GCATATCCAG GAACTGCAGG AGCACAAGGC CCAGCTGGAG 
CAGCAGCTCC AGGGCTTACA CAGGAAGATG GGGGAGACCA GCCTTCTCCT GACCCAGCGG 
GAGCAGGAGA TAGTGGCTCT GCAGCGGCAC CTGCAGGAAG CCAGGGAACA GGGAGAGCTG 
AAAGAGCAGT CACTTCAAGG TCAGCTGGAA GAGGCCCAGA GGGCGCTGGC CCGAAGGGAC 
CAGGAGCTCA AGGCCCTGCA GCAGCAGCAG GCCCAAGGCC AGGAGGAAAA TGTGATGGAA 
AAGGCGGGCT TACTGCAGAG AGAGCTGGAG CAGGCCCACG TGACACTGAA GGAGCGCCAG 
GCGGAGCTTG AGGATTACAA GGAGCATGTG AGAAGGCTCC AGGAAGAGCT GGCAGTGGAG 
GGGCAGCGGG TACAGGCCCT GGAGGAGGTG TTGAGTGACC TAAGGGCTGA ATCTCGGGAA 
CAAGAGAAGG CCCTGCTGGC CCTCCAGCAG CAGTGTGCCG AGCAGGCCCA GGAGCACGAG 
GCAGAGGCCA GGGCCCTGCA GGACAGCTGG CTGCAGGCAG AGGCTATGCT CAAGGAACGG 
GACCAGGAAC TGGAGGCCCT GCGGGCAGAG GGCCTGTCTT CCCAGCATCG GGAGGAGGCT 
GCCTGTGGCC AGGTGGAGGC TCTGCAGGAG GCCCTCAGCA AGGCTCAGGC TGACCTGCAG 
GAGAAAGAGC AGCATCTCCT TGGGCAGGTG GAACTGAGCC GCAGCCTAGA GGCCAGCACG 
GCCACGTTGC AGGCTGCCCT GGACTCCTGC CAGGCACAGT CCCGGCAGCT GGAGGAGGCC 
CTGAGGAAGC GGGAAGGTGA GGTCCAGGTC CGGGACCTCC GATACCAGGA GGCCATGCAG 
CAGCTCCAGC AGGCACTTAC CCAGAGGGAT GAAGAGCTGA GACAACAGAG GAAACAGGGG 
CAGCTCCTGG AGAAGTCTCT GGCCCAGAGG GGCCAAGAGG ATGCAACCCA AGAGAAGCAA 
GAAGCAAAGC ACGAGAGAGA AGAGGAAGAG ATAAGGAGCC TTCGTGAGAG TCTGCAGGAG 
CTACAGCTGA CTCTAGCACA AAAGGAAGAG GAGATCCTGG AGCTGAGGGA GGCCCAGCAG 
AGGAAGAGTC TGGAGGACTT GTCTCACAAA GGCTGCCCAG GGAAGGAGCC GGCTACAACA 
TTTGATTCCT TAGGGCCCAG GCTGCAGCGG GAACTGGAGC GACTACAGGC AGCACTGAAG 
CAGACAGAAG CCAGGGAGAT CGAGTGGAGG GAGAAGGCCC AGGATCTGGC GCTGTCCCTG 
GCCCAGAGCA AGGCCAGTGT CAGCGATCTG CAGGAGGTAG CCCTGTTCCT GCAAGCATCT 
GTTCTGGAGC GGGACTCAGA ACAGCAGAGG CTGCAGGATG AGTTGGAGCT CACCAAACTC 
ACTCTGGAGA AAGAGCGACT CCACAGCCCA GGCCCGAGCA GCAGAGCAGA ACGGGGGCCT 
AGAGGAGAGC CAGGTGTACA ACTGGGAGAG GTCTCGGGAG TGAAGACTGA GCCTAGTCCT 
GAGATGGAGG AGAGGCAGCT CTGGGAACAG AGGCTTGAGC ACCTGCAGCG AGCGGTGGCT 
CTCTTGGAGG TTGACCGAAG CCAGCTGCAA CAACATAACG CCCAGCTGCG GACCACCCTG 
GAGCAGGTGG AGCGAGAGCG GAGGAAACTG AAGAGGGGTT CCATGCGTGC ATTGCGGGCA 
GGGGACCTGG AGAGCGGTGA AGCCACGGCA CCATCACCCA TGCAACAGGA TGGAAGAGGA 
AGGCAGAAGG GCTGCTCAGA TGCCAAGCAC ATGGTTGAGC TGCAAAAAGA GGTGGCCCTC 
CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG CAGAAGCAGG ACTACATTGC TCGCTCAGTT 
CAGACGAGCC GTGAGCTGGC GGACCTGCAC CACGGCCTCT CTCACTCCCT TCTCGCTGTA 
GCCCAAGCCC CCGAGGCCAC CGTCTTAGAG GCCGAGACCC GCAAGCTGGA CGAGTCTCTG 
ACTCAAAGCC TGACATCCCC CGGGCCAGCA CTGCTGTGCC CCAGCTCCAG TGCTGCTCAG 
ACTGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_013985309.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013840763.1 Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X5, mRNA.

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CloneID	OSe44857
Clone ID Related Accession (Same CDS sequence)	XM_013985309.2 , XM_013985309.1
Accession Version	XM_013985309.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	centrosome-associated protein CEP250 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003612568.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACCT TCACAAGGGT GTTGCAGTAC CGGAGCTGGA GCCAAGAGCT GGAGAAGCGG 
CTGGAAGCCA CTGGGGCACC AATCCCTCAG AGGTGGGAAA ATGTGGAGGA GCCAAACCTG 
GAACAGCTGC TAATCCGATT GGAGGAGGAG CAACAGAGGT GTGAGAGTCT AGCAGAGGTG 
AACACCCAGC TTCGGCTGCA CATGGAAAAA GCTGACGTGG TGAATAAAGC GCTTCGGGAA 
GATGTGGAAA AATTGACAGT AGACTGGAGC CAGGCCCGGG ATGAGCTAAT GAGGAAGGAG 
AGCCAGTGGC AGATGGAGCA GGAGTTCTTC AAGGGCTACC TGAAAGGGGA GCATGGTCGC 
CTTCTCAGTC TATGGCGGGA GGTGGTGACC TTCCGACGTC ACTTTCTGGA AATGAAGTCA 
GCCACTGACA GAGACCTGAT GGAGCTCAAG GCTGAGCACG TGAAGCTCTC TGGGTCCCTG 
TTGACCTGTT GTCTGCGCTT GACTGTGGGA GCACCACAGG CCCGGGAATC CACTGGATCT 
GGGAGGCTGA ATGGGAGTGA ACCATCCCAG CTGATGCTGC TACTGTCTAA GACCCAGGAG 
CTGGAGAAGG AAGCCCATAA AAGGAGCCAG GAGTTAATAC AGCTTAAGAG TCAAGGGGAT 
CTGGAGAAGG CTGAGCTTCA GGATCGGGTG ACCGAGCTTT CTGCTCTGCT GACCCAGTCT 
CAGAAGCAGA ATGAAGATTA TGAAAAGATG GTAAAGGCTC TGAGAGAGAC AATGAAGATC 
CTGGAGGCAA ATCATGCAGA GTTAATGGAA CATGAGGCAT CTCTTAGTAG GAATGCCCAA 
GAGGAGAGGC TGTCTTTACA GCAAGTGATC AAGGATATAA CCCAGGCCAT GGTAGAAGAA 
GGGGACCATA TGGCCCAAGG CTCTGGTCAC GAGCACTCCT TGGACTTGGA TCCTAGTGGC 
TTCTTCTCCC AGTTTGATTC CCAGCAGCCA GACAAGGCCC TCACTCTGGT GCGTTCTGTG 
TTGACTCAGA GACGCCAGGC TGTGCAGGAC CTAAAGCAGC AGCTTTCAGG CTGTCAGGAA 
GCTGTGAGCT CCTTGCAGCA GCAGCATCAT CAGTGGGAAG AAGAGGGTGA GGCCTTGAGA 
CAGCGGCTGC AGAAGCTCAC CGGGGAGCGG GACACCCTGG CAGGGCAGAC TGTGGGCCTG 
CAGGGGGAGG TGGACTCTCT GAGCAGGGAG CGAGAGCTCC TGCAAAAGAC CAGGGAGGAG 
CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG GAGGCCTGGC GACTACGGAG GACAAACATG 
GAGCTTCAGC TGCAGGGCGA CTCTGCTCAG GGCGAGAAGG AGGAGCAGCA GGAGGAGCTG 
CACCTGGCTG TCCGAGAACG GGAGCGCCTT CAGGAGATGC TGGCGAGCCT GGAAGCCAAA 
CAGTCAGAAT CTCTCAGTGA ACTGATCACC CTTCGGGAAG CCCTGGAGTC TAGTCGCCTA 
GAAGGGGAGT TGCTGAGGCA AGAGCAAACA GAAGTGACTG CTGTGCTAGC AAAGGCAGAA 
CAGTCTATTG TGGAGCTGTC AAGTTCTGAG AACAGCCTGA AGGCTGAGGT AGCTGATCTT 
CGGGCTGCAG CTGTCAAGCT CAGTGCCTTA AATGAGGCTT TGGCCTTAGA TAAAGTTGGA 
CTGAACCAGC AGCTTCTCCA GTTAGAACAG CAGAACCAGT CTGTGTGCAG CAGGATGGAG 
GCAGCAGAGC AGGCAAGAAG CGCTTTGCAG GTGGGCCTGG CAGAGGTGGA GAGGAGCAGG 
GAAGCGCTGT GGGAAAAGAA CACTTACCTG GAGGCTCAGC TGCAAAAAGC AGAGGCGGCC 
AGGGCTGAGC TGCAGGCAGA TCTCAGGGGC GTCCAGGAAG AGAAGGAAGA AATTCAAGAG 
AAACTAAGCG AGGCACATCA CCAGCAGGAG GCAGTCTCAG TTCAGATGGA GCAGCTGCGT 
CAGGAGGCAA AGCGGCAGGA AGAGGTGCTC GCCCGGGAAG TCCAGGAGAA GGAGGCTCTG 
GTGCGGGAAA AGGCGGCTCT TGAAGTGCGG CTGCAGGCTG TGGAGCGGGA CCGGCAGGAC 
CTCTCTGAAC AACTGCTGGG GCTCAGCTCG GCCAAGGAGC TTCTGGAGAA CAGTCTGTTT 
GAGGCCCAGC AACAGAATTC TCTGATAGAA GTCACCAAGG GGCAGCTGGA GGTCCAGATT 
CAAACTATCA CTCAAGCCAA GGAGGTAATC CAAGGGGAAG TGAGGTGCCT ACAGCTGGAA 
CTGGATACTG AACGGAGCCG GGCAGAACAG GAGCGGGATG CTGCAACCAA ACAGCTGGCC 
CAGGCTGAGC AAGAGGGGCA GACCGCCCTG CAGCAGCAGA AGTCAGCCCA TGAGGAGGAA 
GTGAAGGGGC TCCAGGAAAA ATGGGAGAAA GAGCGTGCCT GGCACCAGCA GGAACTGGAT 
AAGGCTCTGA AGAGCCTTGA GAGGGAGAAA ACGGAGCTGG AAATGAGGCT GAGGGAGCAG 
CAGGCAGAAA CAGAGGCCAT GCGGACGCAG AGGGAAGAAG AACGGGCTGA GGCTGAGAGC 
ACCCTGCGCC AGATGCAGCT CGAAACAGAG AAGGAGAGAA TGTCCCTCCT GGAGACACTG 
CTGCAGACCC AGAAGGAGCT GGCAGATGCC AGCCAACAAC TGGAGCGGCT GAGGCAGGAC 
ATGAAGGTTC AGAAGTTAAA GGAGCAGGAG ACCACTGGGA TGCTGCAGAC CCAGCTCCGG 
GAAGCTCAGA AGGAGCTGGA ACAGGCAGCC CAGCAGCACA GAGACAGCCT TGCTGCCCTC 
CAAGAAGAAG GCCGTGCCCT GCTGCAGGAT AAGATAGACC TGCACAAGCA GGTGGAGGAC 
TTGAAGTCTC AGCTGGTTTC CAAGGAAGAC TCCCAAAGGC TGGTAGACCA GGAAGTTCAG 
GAGAAGCTGA GAGAGGCCCA GGAATGTAGC CGAATTCAGA AGGAGCTGGA GAAAGAAAAG 
GCCAGCCTGA CTCTGTTGCT GGTGGAAAAG GAACAGAGAC TCCTTGGTTT ACAAGAGGCC 
GACTCCATTC GACAACAAGA GCTGAGCTCC CTGCGCCAGG ACATGCAGGA GGCCCAGGCA 
GGGCAGAAAG AGCTCAGCGC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGCAAAGGAG 
ACTGACTTTC TGGCCCAGGA AGCGCGGCTG CTGGAAGAGC TGGAGGCATC TCAAGTAACA 
GAGCAGCAGC TACGAGCTTC CTTGTGGGCC CAGGAAGCCA AGGCCGCACA ACTACAGCTG 
CGACTGCGCA GCACAGAGAG CCAGCTGGAG GTGCTGCTGG CAGAGCAGCA GCCTGGGCAC 
CAGGCCCAGG CCCAGCTGGC CAGCCTTTGT GCTGTCCTGC AGCAGGCCCT GGGGTCTGTT 
TGTGAGAGCA GGCCTGAGCT GAGTGGCGGG GGAGACTCTG CTCCCTCCCT CTGGGGCCCT 
GAGCCAGACC AGAATGGAGC TAGGACCCTT TTCAAGAGAG GGCCCCTCCT GACAGCTCTC 
TCGGCTGAGG CGGTGGCCTC TGCCCTTCAC AAGCTTAACC AAGACCTGTG GAAGACTCAG 
CAGGCCCGGG ATGATCTGAG GGATCAGGCC CAGAAGCTGG GACAACGTCT TACTGAAACA 
GAGGCCGAGA AGAGCCAGCT CCACACAGAG TTGCAGGCTC TGCAGAGGCA GCTCTCCCAG 
AACCAGGAAG AGAGATCCAA GTGGGAAGGA AAACAGACCT CCTTAGAATC TGAGCTGGTG 
GAACTGCATG AAACTGTGAC ATCCTTACAG AGTCGCCTAC GGCAAGCAGA GCTGCAGGGA 
ATAGAAGCCC AGAATGAGCA AGAGTTACTG CAGGCAGCCA AGGAGAACCT GACAGCCCAG 
GTGGAACACC TCCAAGCAGC TGTTGCAGAA GCCAGGGCTC AGGCAAGCGC TGCCGAGGTC 
CTGGAGGAAG ACCTCAAAAC TGCACGCTCA GCCCTGAAAC TGAAAAGCAA GGAGGTGGAG 
AGTGAGCGTG AGCGAGCCCA GGCCCTACAG GAGCAGGGCG AGCTGAAGGT GGCCCAAGGG 
AAAGCTTTGC AGGAGAATTT GGCTCGCCTG ACTCAGACCC TATCTGAGAG AGAAGGGGAG 
GTGGAAACTT TGCAGGGAAA AATCCAGGAG CTGGAGAAGC AACGGGAAAT GCAAAAGGCT 
GCTTTGGAGG TGCTGTCTTT GGACCTCAAA AAGAGGAACC AAGAGGTGGA TCTGCAGCAA 
GAACAGATTC AGGAGCTGGA GGAGTGCAGA TCGGTTTTAG AGCATCTCCC CATGGCAGTC 
CAGGAGCGAG AGCAGAAGCT GACTCTGCAG AGGGAGCAGA TCAAGGAGCT CGAGAAGGAC 
CGAGAGACAC AGAGGAACAT CTTGGAGCAT CAGCTTCTGG AACTTGAGAA GAAGGCCCAA 
GTGATTGAGT CCCAGAAAGG ACAGATTCAG GATCTGAAGA AGCAGCTGGT TACTCTGGAA 
TGCCTGGCCC TGGAACTAGA GGAAAACCAT CACAAAATAG AGTGCCAGCA AAAGACAATT 
GAGGAGCTGG AGGGCCAGAG GGAAATGCAG AGGGCAGCTC TGACCCATCT GACACTGGAC 
CTGGAGGAAA GGAGCCAGGA GCTGCAGGTA CAGGGCAGCC AGATCCGGGA GCTAGAGAGT 
CACAACAGCC TCTTGGCAAG AGAGCTCCAG GAGAAGGACC AGGAGATGAC GTCCCAGCAA 
GACCAGACAG AGGAACTGCA GAGGCAGAAG GAGCATTTGA CACAGGACCT CAAGAGGCGG 
GAACAGGAGA TAGTGCTGCT GAAGGAGAGG ATTCAGGTCT TGGAAGATCA GAGAACCCTA 
CAGACCAAGA TCCTAGAGGA GGACCTGGAG CAGATCAAAA TGTCCTTGAG GGAGCGAGGC 
CGGGACCTGG CCTCGCAGAG GCAGCTGATG CAGGAGCGGG CAGAGGAAGG GAAGGGCCAG 
AGTAAAGCAC AGCGCGGGAG CCTGGAGCAT ATGAAGCTGA TCCTGCGTGA TAAGGAGAAG 
GAGGTGGAAT GCCAGCAGGA GCATATCCAG GAACTGCAGG AGCACAAGGC CCAGCTGGAG 
CAGCAGCTCC AGGGCTTACA CAGGAAGATG GGGGAGACCA GCCTTCTCCT GACCCAGCGG 
GAGCAGGAGA TAGTGGCTCT GCAGCGGCAC CTGCAGGAAG CCAGGGAACA GGGAGAGCTG 
AAAGAGCAGT CACTTCAAGG TCAGCTGGAA GAGGCCCAGA GGGCGCTGGC CCGAAGGGAC 
CAGGAGCTCA AGGCCCTGCA GCAGCAGCAG GCCCAAGGCC AGGAGGAAAA TGTGATGGAA 
AAGGCGGGCT TACTGCAGAG AGAGCTGGAG CAGGCCCACG TGACACTGAA GGAGCGCCAG 
GCGGAGCTTG AGGATTACAA GGAGCATGTG AGAAGGCTCC AGGAAGAGCT GGCAGTGGAG 
GGGCAGCGGG TACAGGCCCT GGAGGAGGTG TTGAGTGACC TAAGGGCTGA ATCTCGGGAA 
CAAGAGAAGG CCCTGCTGGC CCTCCAGCAG CAGTGTGCCG AGCAGGCCCA GGAGCACGAG 
GCAGAGGCCA GGGCCCTGCA GGACAGCTGG CTGCAGGCAG AGGCTATGCT CAAGGAACGG 
GACCAGGAAC TGGAGGCCCT GCGGGCAGAG GGCCTGTCTT CCCAGCATCG GGAGGAGGCT 
GCCTGTGGCC AGGTGGAGGC TCTGCAGGAG GCCCTCAGCA AGGCTCAGGC TGACCTGCAG 
GAGAAAGAGC AGCATCTCCT TGGGCAGGTG GAACTGAGCC GCAGCCTAGA GGCCAGCACG 
GCCACGTTGC AGGCTGCCCT GGACTCCTGC CAGGCACAGT CCCGGCAGCT GGAGGAGGCC 
CTGAGGAAGC GGGAAGGTGA GGTCCAGGTC CGGGACCTCC GATACCAGGA GGCCATGCAG 
CAGCTCCAGC AGGCACTTAC CCAGAGGGAT GAAGAGCTGA GACAACAGAG GAAACAGGGG 
CAGCTCCTGG AGAAGTCTCT GGCCCAGAGG GGCCAAGAGG ATGCAACCCA AGAGAAGCAA 
GAAGCAAAGC ACGAGAGAGA AGAGGAAGAG ATAAGGAGCC TTCGTGAGAG TCTGCAGGAG 
CTACAGCTGA CTCTAGCACA AAAGGAAGAG GAGATCCTGG AGCTGAGGGA GGCCCAGCAG 
AGGAAGAGTC TGGAGGACTT GTCTCACAAA GGCTGCCCAG GGAAGGAGCC GGCTACAACA 
TTTGATTCCT TAGGGCCCAG GCTGCAGCGG GAACTGGAGC GACTACAGGC AGCACTGAAG 
CAGACAGAAG CCAGGGAGAT CGAGTGGAGG GAGAAGGCCC AGGATCTGGC GCTGTCCCTG 
GCCCAGAGCA AGGCCAGTGT CAGCGATCTG CAGGAGGTAG CCCTGTTCCT GCAAGCATCT 
GTTCTGGAGC GGGACTCAGA ACAGCAGAGG CTGCAGGATG AGTTGGAGCT CACCAAACTC 
ACTCTGGAGA AAGAGCGACT CCACAGCCCA GGCCCGAGCA GCAGAGCAGA ACGGGGGCCT 
AGAGGAGAGC CAGGTGTACA ACTGGGAGAG GTCTCGGGAG TGAAGACTGA GCCTAGTCCT 
GAGATGGAGG AGAGGCAGCT CTGGGAACAG AGGCTTGAGC ACCTGCAGCG AGCGGTGGCT 
CTCTTGGAGG TTGACCGAAG CCAGCTGCAA CAACATAACG CCCAGCTGCG GACCACCCTG 
GAGCAGGTGG AGCGAGAGCG GAGGAAACTG AAGAGGGGTT CCATGCGTGC ATTGCGGGCA 
GGGGACCTGG AGAGCGGTGA AGCCACGGCA CCATCACCCA TGCAACAGGA TGGAAGAGGA 
AGGCAGAAGG GCTGCTCAGA TGCCAAGCAC ATGGTTGAGC TGCAAAAAGA GGTGGCCCTC 
CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG CAGAAGCAGG ACTACATTGC TCGCTCAGTT 
CAGACGAGCC GTGAGCTGGC GGACCTGCAC CACGGCCTCT CTCACTCCCT TCTCGCTGTA 
GCCCAAGCCC CCGAGGCCAC CGTCTTAGAG GCCGAGACCC GCAAGCTGGA CGAGTCTCTG 
ACTCAAAGCC TGACATCCCC CGGGCCAGCA CTGCTGTGCC CCAGCTCCAG TGCTGCTCAG 
ACTGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013840763.1
CDS	91..7245
Translation	MMTFTRVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_013985309.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013985309.1

ORF Insert Sequence:

ATGATGACCT TCACAAGGGT GTTGCAGTAC CGGAGCTGGA GCCAAGAGCT GGAGAAGCGG 
CTGGAAGCCA CTGGGGCACC AATCCCTCAG AGGTGGGAAA ATGTGGAGGA GCCAAACCTG 
GAACAGCTGC TAATCCGATT GGAGGAGGAG CAACAGAGGT GTGAGAGTCT AGCAGAGGTG 
AACACCCAGC TTCGGCTGCA CATGGAAAAA GCTGACGTGG TGAATAAAGC GCTTCGGGAA 
GATGTGGAAA AATTGACAGT AGACTGGAGC CAGGCCCGGG ATGAGCTAAT GAGGAAGGAG 
AGCCAGTGGC AGATGGAGCA GGAGTTCTTC AAGGGCTACC TGAAAGGGGA GCATGGTCGC 
CTTCTCAGTC TATGGCGGGA GGTGGTGACC TTCCGACGTC ACTTTCTGGA AATGAAGTCA 
GCCACTGACA GAGACCTGAT GGAGCTCAAG GCTGAGCACG TGAAGCTCTC TGGGTCCCTG 
TTGACCTGTT GTCTGCGCTT GACTGTGGGA GCACCACAGG CCCGGGAATC CACTGGATCT 
GGGAGGCTGA ATGGGAGTGA ACCATCCCAG CTGATGCTGC TACTGTCTAA GACCCAGGAG 
CTGGAGAAGG AAGCCCATAA AAGGAGCCAG GAGTTAATAC AGCTTAAGAG TCAAGGGGAT 
CTGGAGAAGG CTGAGCTTCA GGATCGGGTG ACCGAGCTTT CTGCTCTGCT GACCCAGTCT 
CAGAAGCAGA ATGAAGATTA TGAAAAGATG GTAAAGGCTC TGAGAGAGAC AATGAAGATC 
CTGGAGGCAA ATCATGCAGA GTTAATGGAA CATGAGGCAT CTCTTAGTAG GAATGCCCAA 
GAGGAGAGGC TGTCTTTACA GCAAGTGATC AAGGATATAA CCCAGGCCAT GGTAGAAGAA 
GGGGACCATA TGGCCCAAGG CTCTGGTCAC GAGCACTCCT TGGACTTGGA TCCTAGTGGC 
TTCTTCTCCC AGTTTGATTC CCAGCAGCCA GACAAGGCCC TCACTCTGGT GCGTTCTGTG 
TTGACTCAGA GACGCCAGGC TGTGCAGGAC CTAAAGCAGC AGCTTTCAGG CTGTCAGGAA 
GCTGTGAGCT CCTTGCAGCA GCAGCATCAT CAGTGGGAAG AAGAGGGTGA GGCCTTGAGA 
CAGCGGCTGC AGAAGCTCAC CGGGGAGCGG GACACCCTGG CAGGGCAGAC TGTGGGCCTG 
CAGGGGGAGG TGGACTCTCT GAGCAGGGAG CGAGAGCTCC TGCAAAAGAC CAGGGAGGAG 
CTGCAGCAGC AGCTGGAGGT GCTGGAGCAG GAGGCCTGGC GACTACGGAG GACAAACATG 
GAGCTTCAGC TGCAGGGCGA CTCTGCTCAG GGCGAGAAGG AGGAGCAGCA GGAGGAGCTG 
CACCTGGCTG TCCGAGAACG GGAGCGCCTT CAGGAGATGC TGGCGAGCCT GGAAGCCAAA 
CAGTCAGAAT CTCTCAGTGA ACTGATCACC CTTCGGGAAG CCCTGGAGTC TAGTCGCCTA 
GAAGGGGAGT TGCTGAGGCA AGAGCAAACA GAAGTGACTG CTGTGCTAGC AAAGGCAGAA 
CAGTCTATTG TGGAGCTGTC AAGTTCTGAG AACAGCCTGA AGGCTGAGGT AGCTGATCTT 
CGGGCTGCAG CTGTCAAGCT CAGTGCCTTA AATGAGGCTT TGGCCTTAGA TAAAGTTGGA 
CTGAACCAGC AGCTTCTCCA GTTAGAACAG CAGAACCAGT CTGTGTGCAG CAGGATGGAG 
GCAGCAGAGC AGGCAAGAAG CGCTTTGCAG GTGGGCCTGG CAGAGGTGGA GAGGAGCAGG 
GAAGCGCTGT GGGAAAAGAA CACTTACCTG GAGGCTCAGC TGCAAAAAGC AGAGGCGGCC 
AGGGCTGAGC TGCAGGCAGA TCTCAGGGGC GTCCAGGAAG AGAAGGAAGA AATTCAAGAG 
AAACTAAGCG AGGCACATCA CCAGCAGGAG GCAGTCTCAG TTCAGATGGA GCAGCTGCGT 
CAGGAGGCAA AGCGGCAGGA AGAGGTGCTC GCCCGGGAAG TCCAGGAGAA GGAGGCTCTG 
GTGCGGGAAA AGGCGGCTCT TGAAGTGCGG CTGCAGGCTG TGGAGCGGGA CCGGCAGGAC 
CTCTCTGAAC AACTGCTGGG GCTCAGCTCG GCCAAGGAGC TTCTGGAGAA CAGTCTGTTT 
GAGGCCCAGC AACAGAATTC TCTGATAGAA GTCACCAAGG GGCAGCTGGA GGTCCAGATT 
CAAACTATCA CTCAAGCCAA GGAGGTAATC CAAGGGGAAG TGAGGTGCCT ACAGCTGGAA 
CTGGATACTG AACGGAGCCG GGCAGAACAG GAGCGGGATG CTGCAACCAA ACAGCTGGCC 
CAGGCTGAGC AAGAGGGGCA GACCGCCCTG CAGCAGCAGA AGTCAGCCCA TGAGGAGGAA 
GTGAAGGGGC TCCAGGAAAA ATGGGAGAAA GAGCGTGCCT GGCACCAGCA GGAACTGGAT 
AAGGCTCTGA AGAGCCTTGA GAGGGAGAAA ACGGAGCTGG AAATGAGGCT GAGGGAGCAG 
CAGGCAGAAA CAGAGGCCAT GCGGACGCAG AGGGAAGAAG AACGGGCTGA GGCTGAGAGC 
ACCCTGCGCC AGATGCAGCT CGAAACAGAG AAGGAGAGAA TGTCCCTCCT GGAGACACTG 
CTGCAGACCC AGAAGGAGCT GGCAGATGCC AGCCAACAAC TGGAGCGGCT GAGGCAGGAC 
ATGAAGGTTC AGAAGTTAAA GGAGCAGGAG ACCACTGGGA TGCTGCAGAC CCAGCTCCGG 
GAAGCTCAGA AGGAGCTGGA ACAGGCAGCC CAGCAGCACA GAGACAGCCT TGCTGCCCTC 
CAAGAAGAAG GCCGTGCCCT GCTGCAGGAT AAGATAGACC TGCACAAGCA GGTGGAGGAC 
TTGAAGTCTC AGCTGGTTTC CAAGGAAGAC TCCCAAAGGC TGGTAGACCA GGAAGTTCAG 
GAGAAGCTGA GAGAGGCCCA GGAATGTAGC CGAATTCAGA AGGAGCTGGA GAAAGAAAAG 
GCCAGCCTGA CTCTGTTGCT GGTGGAAAAG GAACAGAGAC TCCTTGGTTT ACAAGAGGCC 
GACTCCATTC GACAACAAGA GCTGAGCTCC CTGCGCCAGG ACATGCAGGA GGCCCAGGCA 
GGGCAGAAAG AGCTCAGCGC CCAGGTGGAA TTACTGAAGC AGGAGGTGAA GGCAAAGGAG 
ACTGACTTTC TGGCCCAGGA AGCGCGGCTG CTGGAAGAGC TGGAGGCATC TCAAGTAACA 
GAGCAGCAGC TACGAGCTTC CTTGTGGGCC CAGGAAGCCA AGGCCGCACA ACTACAGCTG 
CGACTGCGCA GCACAGAGAG CCAGCTGGAG GTGCTGCTGG CAGAGCAGCA GCCTGGGCAC 
CAGGCCCAGG CCCAGCTGGC CAGCCTTTGT GCTGTCCTGC AGCAGGCCCT GGGGTCTGTT 
TGTGAGAGCA GGCCTGAGCT GAGTGGCGGG GGAGACTCTG CTCCCTCCCT CTGGGGCCCT 
GAGCCAGACC AGAATGGAGC TAGGACCCTT TTCAAGAGAG GGCCCCTCCT GACAGCTCTC 
TCGGCTGAGG CGGTGGCCTC TGCCCTTCAC AAGCTTAACC AAGACCTGTG GAAGACTCAG 
CAGGCCCGGG ATGATCTGAG GGATCAGGCC CAGAAGCTGG GACAACGTCT TACTGAAACA 
GAGGCCGAGA AGAGCCAGCT CCACACAGAG TTGCAGGCTC TGCAGAGGCA GCTCTCCCAG 
AACCAGGAAG AGAGATCCAA GTGGGAAGGA AAACAGACCT CCTTAGAATC TGAGCTGGTG 
GAACTGCATG AAACTGTGAC ATCCTTACAG AGTCGCCTAC GGCAAGCAGA GCTGCAGGGA 
ATAGAAGCCC AGAATGAGCA AGAGTTACTG CAGGCAGCCA AGGAGAACCT GACAGCCCAG 
GTGGAACACC TCCAAGCAGC TGTTGCAGAA GCCAGGGCTC AGGCAAGCGC TGCCGAGGTC 
CTGGAGGAAG ACCTCAAAAC TGCACGCTCA GCCCTGAAAC TGAAAAGCAA GGAGGTGGAG 
AGTGAGCGTG AGCGAGCCCA GGCCCTACAG GAGCAGGGCG AGCTGAAGGT GGCCCAAGGG 
AAAGCTTTGC AGGAGAATTT GGCTCGCCTG ACTCAGACCC TATCTGAGAG AGAAGGGGAG 
GTGGAAACTT TGCAGGGAAA AATCCAGGAG CTGGAGAAGC AACGGGAAAT GCAAAAGGCT 
GCTTTGGAGG TGCTGTCTTT GGACCTCAAA AAGAGGAACC AAGAGGTGGA TCTGCAGCAA 
GAACAGATTC AGGAGCTGGA GGAGTGCAGA TCGGTTTTAG AGCATCTCCC CATGGCAGTC 
CAGGAGCGAG AGCAGAAGCT GACTCTGCAG AGGGAGCAGA TCAAGGAGCT CGAGAAGGAC 
CGAGAGACAC AGAGGAACAT CTTGGAGCAT CAGCTTCTGG AACTTGAGAA GAAGGCCCAA 
GTGATTGAGT CCCAGAAAGG ACAGATTCAG GATCTGAAGA AGCAGCTGGT TACTCTGGAA 
TGCCTGGCCC TGGAACTAGA GGAAAACCAT CACAAAATAG AGTGCCAGCA AAAGACAATT 
GAGGAGCTGG AGGGCCAGAG GGAAATGCAG AGGGCAGCTC TGACCCATCT GACACTGGAC 
CTGGAGGAAA GGAGCCAGGA GCTGCAGGTA CAGGGCAGCC AGATCCGGGA GCTAGAGAGT 
CACAACAGCC TCTTGGCAAG AGAGCTCCAG GAGAAGGACC AGGAGATGAC GTCCCAGCAA 
GACCAGACAG AGGAACTGCA GAGGCAGAAG GAGCATTTGA CACAGGACCT CAAGAGGCGG 
GAACAGGAGA TAGTGCTGCT GAAGGAGAGG ATTCAGGTCT TGGAAGATCA GAGAACCCTA 
CAGACCAAGA TCCTAGAGGA GGACCTGGAG CAGATCAAAA TGTCCTTGAG GGAGCGAGGC 
CGGGACCTGG CCTCGCAGAG GCAGCTGATG CAGGAGCGGG CAGAGGAAGG GAAGGGCCAG 
AGTAAAGCAC AGCGCGGGAG CCTGGAGCAT ATGAAGCTGA TCCTGCGTGA TAAGGAGAAG 
GAGGTGGAAT GCCAGCAGGA GCATATCCAG GAACTGCAGG AGCACAAGGC CCAGCTGGAG 
CAGCAGCTCC AGGGCTTACA CAGGAAGATG GGGGAGACCA GCCTTCTCCT GACCCAGCGG 
GAGCAGGAGA TAGTGGCTCT GCAGCGGCAC CTGCAGGAAG CCAGGGAACA GGGAGAGCTG 
AAAGAGCAGT CACTTCAAGG TCAGCTGGAA GAGGCCCAGA GGGCGCTGGC CCGAAGGGAC 
CAGGAGCTCA AGGCCCTGCA GCAGCAGCAG GCCCAAGGCC AGGAGGAAAA TGTGATGGAA 
AAGGCGGGCT TACTGCAGAG AGAGCTGGAG CAGGCCCACG TGACACTGAA GGAGCGCCAG 
GCGGAGCTTG AGGATTACAA GGAGCATGTG AGAAGGCTCC AGGAAGAGCT GGCAGTGGAG 
GGGCAGCGGG TACAGGCCCT GGAGGAGGTG TTGAGTGACC TAAGGGCTGA ATCTCGGGAA 
CAAGAGAAGG CCCTGCTGGC CCTCCAGCAG CAGTGTGCCG AGCAGGCCCA GGAGCACGAG 
GCAGAGGCCA GGGCCCTGCA GGACAGCTGG CTGCAGGCAG AGGCTATGCT CAAGGAACGG 
GACCAGGAAC TGGAGGCCCT GCGGGCAGAG GGCCTGTCTT CCCAGCATCG GGAGGAGGCT 
GCCTGTGGCC AGGTGGAGGC TCTGCAGGAG GCCCTCAGCA AGGCTCAGGC TGACCTGCAG 
GAGAAAGAGC AGCATCTCCT TGGGCAGGTG GAACTGAGCC GCAGCCTAGA GGCCAGCACG 
GCCACGTTGC AGGCTGCCCT GGACTCCTGC CAGGCACAGT CCCGGCAGCT GGAGGAGGCC 
CTGAGGAAGC GGGAAGGTGA GGTCCAGGTC CGGGACCTCC GATACCAGGA GGCCATGCAG 
CAGCTCCAGC AGGCACTTAC CCAGAGGGAT GAAGAGCTGA GACAACAGAG GAAACAGGGG 
CAGCTCCTGG AGAAGTCTCT GGCCCAGAGG GGCCAAGAGG ATGCAACCCA AGAGAAGCAA 
GAAGCAAAGC ACGAGAGAGA AGAGGAAGAG ATAAGGAGCC TTCGTGAGAG TCTGCAGGAG 
CTACAGCTGA CTCTAGCACA AAAGGAAGAG GAGATCCTGG AGCTGAGGGA GGCCCAGCAG 
AGGAAGAGTC TGGAGGACTT GTCTCACAAA GGCTGCCCAG GGAAGGAGCC GGCTACAACA 
TTTGATTCCT TAGGGCCCAG GCTGCAGCGG GAACTGGAGC GACTACAGGC AGCACTGAAG 
CAGACAGAAG CCAGGGAGAT CGAGTGGAGG GAGAAGGCCC AGGATCTGGC GCTGTCCCTG 
GCCCAGAGCA AGGCCAGTGT CAGCGATCTG CAGGAGGTAG CCCTGTTCCT GCAAGCATCT 
GTTCTGGAGC GGGACTCAGA ACAGCAGAGG CTGCAGGATG AGTTGGAGCT CACCAAACTC 
ACTCTGGAGA AAGAGCGACT CCACAGCCCA GGCCCGAGCA GCAGAGCAGA ACGGGGGCCT 
AGAGGAGAGC CAGGTGTACA ACTGGGAGAG GTCTCGGGAG TGAAGACTGA GCCTAGTCCT 
GAGATGGAGG AGAGGCAGCT CTGGGAACAG AGGCTTGAGC ACCTGCAGCG AGCGGTGGCT 
CTCTTGGAGG TTGACCGAAG CCAGCTGCAA CAACATAACG CCCAGCTGCG GACCACCCTG 
GAGCAGGTGG AGCGAGAGCG GAGGAAACTG AAGAGGGGTT CCATGCGTGC ATTGCGGGCA 
GGGGACCTGG AGAGCGGTGA AGCCACGGCA CCATCACCCA TGCAACAGGA TGGAAGAGGA 
AGGCAGAAGG GCTGCTCAGA TGCCAAGCAC ATGGTTGAGC TGCAAAAAGA GGTGGCCCTC 
CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG CAGAAGCAGG ACTACATTGC TCGCTCAGTT 
CAGACGAGCC GTGAGCTGGC GGACCTGCAC CACGGCCTCT CTCACTCCCT TCTCGCTGTA 
GCCCAAGCCC CCGAGGCCAC CGTCTTAGAG GCCGAGACCC GCAAGCTGGA CGAGTCTCTG 
ACTCAAAGCC TGACATCCCC CGGGCCAGCA CTGCTGTGCC CCAGCTCCAG TGCTGCTCAG 
ACTGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_013985310.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013840764.1 Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X6, mRNA.

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CloneID	OSe44858
Clone ID Related Accession (Same CDS sequence)	XM_013985310.1
Accession Version	XM_013985310.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	centrosome-associated protein CEP250 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003612568.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGG CACATCACCA GCAGGAGGCA GTCTCAGTTC AGATGGAGCA GCTGCGTCAG 
GAGGCAAAGC GGCAGGAAGA GGTGCTCGCC CGGGAAGTCC AGGAGAAGGA GGCTCTGGTG 
CGGGAAAAGG CGGCTCTTGA AGTGCGGCTG CAGGCTGTGG AGCGGGACCG GCAGGACCTC 
TCTGAACAAC TGCTGGGGCT CAGCTCGGCC AAGGAGCTTC TGGAGAACAG TCTGTTTGAG 
GCCCAGCAAC AGAATTCTCT GATAGAAGTC ACCAAGGGGC AGCTGGAGGT CCAGATTCAA 
ACTATCACTC AAGCCAAGGA GGTAATCCAA GGGGAAGTGA GGTGCCTACA GCTGGAACTG 
GATACTGAAC GGAGCCGGGC AGAACAGGAG CGGGATGCTG CAACCAAACA GCTGGCCCAG 
GCTGAGCAAG AGGGGCAGAC CGCCCTGCAG CAGCAGAAGT CAGCCCATGA GGAGGAAGTG 
AAGGGGCTCC AGGAAAAATG GGAGAAAGAG CGTGCCTGGC ACCAGCAGGA ACTGGATAAG 
GCTCTGAAGA GCCTTGAGAG GGAGAAAACG GAGCTGGAAA TGAGGCTGAG GGAGCAGCAG 
GCAGAAACAG AGGCCATGCG GACGCAGAGG GAAGAAGAAC GGGCTGAGGC TGAGAGCACC 
CTGCGCCAGA TGCAGCTCGA AACAGAGAAG GAGAGAATGT CCCTCCTGGA GACACTGCTG 
CAGACCCAGA AGGAGCTGGC AGATGCCAGC CAACAACTGG AGCGGCTGAG GCAGGACATG 
AAGGTTCAGA AGTTAAAGGA GCAGGAGACC ACTGGGATGC TGCAGACCCA GCTCCGGGAA 
GCTCAGAAGG AGCTGGAACA GGCAGCCCAG CAGCACAGAG ACAGCCTTGC TGCCCTCCAA 
GAAGAAGGCC GTGCCCTGCT GCAGGATAAG ATAGACCTGC ACAAGCAGGT GGAGGACTTG 
AAGTCTCAGC TGGTTTCCAA GGAAGACTCC CAAAGGCTGG TAGACCAGGA AGTTCAGGAG 
AAGCTGAGAG AGGCCCAGGA ATGTAGCCGA ATTCAGAAGG AGCTGGAGAA AGAAAAGGCC 
AGCCTGACTC TGTTGCTGGT GGAAAAGGAA CAGAGACTCC TTGGTTTACA AGAGGCCGAC 
TCCATTCGAC AACAAGAGCT GAGCTCCCTG CGCCAGGACA TGCAGGAGGC CCAGGCAGGG 
CAGAAAGAGC TCAGCGCCCA GGTGGAATTA CTGAAGCAGG AGGTGAAGGC AAAGGAGACT 
GACTTTCTGG CCCAGGAAGC GCGGCTGCTG GAAGAGCTGG AGGCATCTCA AGTAACAGAG 
CAGCAGCTAC GAGCTTCCTT GTGGGCCCAG GAAGCCAAGG CCGCACAACT ACAGCTGCGA 
CTGCGCAGCA CAGAGAGCCA GCTGGAGGTG CTGCTGGCAG AGCAGCAGCC TGGGCACCAG 
GCCCAGGCCC AGCTGGCCAG CCTTTGTGCT GTCCTGCAGC AGGCCCTGGG GTCTGTTTGT 
GAGAGCAGGC CTGAGCTGAG TGGCGGGGGA GACTCTGCTC CCTCCCTCTG GGGCCCTGAG 
CCAGACCAGA ATGGAGCTAG GACCCTTTTC AAGAGAGGGC CCCTCCTGAC AGCTCTCTCG 
GCTGAGGCGG TGGCCTCTGC CCTTCACAAG CTTAACCAAG ACCTGTGGAA GACTCAGCAG 
GCCCGGGATG ATCTGAGGGA TCAGGCCCAG AAGCTGGGAC AACGTCTTAC TGAAACAGAG 
GCCGAGAAGA GCCAGCTCCA CACAGAGTTG CAGGCTCTGC AGAGGCAGCT CTCCCAGAAC 
CAGGAAGAGA GATCCAAGTG GGAAGGAAAA CAGACCTCCT TAGAATCTGA GCTGGTGGAA 
CTGCATGAAA CTGTGACATC CTTACAGAGT CGCCTACGGC AAGCAGAGCT GCAGGGAATA 
GAAGCCCAGA ATGAGCAAGA GTTACTGCAG GCAGCCAAGG AGAACCTGAC AGCCCAGGTG 
GAACACCTCC AAGCAGCTGT TGCAGAAGCC AGGGCTCAGG CAAGCGCTGC CGAGGTCCTG 
GAGGAAGACC TCAAAACTGC ACGCTCAGCC CTGAAACTGA AAAGCAAGGA GGTGGAGAGT 
GAGCGTGAGC GAGCCCAGGC CCTACAGGAG CAGGGCGAGC TGAAGGTGGC CCAAGGGAAA 
GCTTTGCAGG AGAATTTGGC TCGCCTGACT CAGACCCTAT CTGAGAGAGA AGGGGAGGTG 
GAAACTTTGC AGGGAAAAAT CCAGGAGCTG GAGAAGCAAC GGGAAATGCA AAAGGCTGCT 
TTGGAGGTGC TGTCTTTGGA CCTCAAAAAG AGGAACCAAG AGGTGGATCT GCAGCAAGAA 
CAGATTCAGG AGCTGGAGGA GTGCAGATCG GTTTTAGAGC ATCTCCCCAT GGCAGTCCAG 
GAGCGAGAGC AGAAGCTGAC TCTGCAGAGG GAGCAGATCA AGGAGCTCGA GAAGGACCGA 
GAGACACAGA GGAACATCTT GGAGCATCAG CTTCTGGAAC TTGAGAAGAA GGCCCAAGTG 
ATTGAGTCCC AGAAAGGACA GATTCAGGAT CTGAAGAAGC AGCTGGTTAC TCTGGAATGC 
CTGGCCCTGG AACTAGAGGA AAACCATCAC AAAATAGAGT GCCAGCAAAA GACAATTGAG 
GAGCTGGAGG GCCAGAGGGA AATGCAGAGG GCAGCTCTGA CCCATCTGAC ACTGGACCTG 
GAGGAAAGGA GCCAGGAGCT GCAGGTACAG GGCAGCCAGA TCCGGGAGCT AGAGAGTCAC 
AACAGCCTCT TGGCAAGAGA GCTCCAGGAG AAGGACCAGG AGATGACGTC CCAGCAAGAC 
CAGACAGAGG AACTGCAGAG GCAGAAGGAG CATTTGACAC AGGACCTCAA GAGGCGGGAA 
CAGGAGATAG TGCTGCTGAA GGAGAGGATT CAGGTCTTGG AAGATCAGAG AACCCTACAG 
ACCAAGATCC TAGAGGAGGA CCTGGAGCAG ATCAAAATGT CCTTGAGGGA GCGAGGCCGG 
GACCTGGCCT CGCAGAGGCA GCTGATGCAG GAGCGGGCAG AGGAAGGGAA GGGCCAGAGT 
AAAGCACAGC GCGGGAGCCT GGAGCATATG AAGCTGATCC TGCGTGATAA GGAGAAGGAG 
GTGGAATGCC AGCAGGAGCA TATCCAGGAA CTGCAGGAGC ACAAGGCCCA GCTGGAGCAG 
CAGCTCCAGG GCTTACACAG GAAGATGGGG GAGACCAGCC TTCTCCTGAC CCAGCGGGAG 
CAGGAGATAG TGGCTCTGCA GCGGCACCTG CAGGAAGCCA GGGAACAGGG AGAGCTGAAA 
GAGCAGTCAC TTCAAGGTCA GCTGGAAGAG GCCCAGAGGG CGCTGGCCCG AAGGGACCAG 
GAGCTCAAGG CCCTGCAGCA GCAGCAGGCC CAAGGCCAGG AGGAAAATGT GATGGAAAAG 
GCGGGCTTAC TGCAGAGAGA GCTGGAGCAG GCCCACGTGA CACTGAAGGA GCGCCAGGCG 
GAGCTTGAGG ATTACAAGGA GCATGTGAGA AGGCTCCAGG AAGAGCTGGC AGTGGAGGGG 
CAGCGGGTAC AGGCCCTGGA GGAGGTGTTG AGTGACCTAA GGGCTGAATC TCGGGAACAA 
GAGAAGGCCC TGCTGGCCCT CCAGCAGCAG TGTGCCGAGC AGGCCCAGGA GCACGAGGCA 
GAGGCCAGGG CCCTGCAGGA CAGCTGGCTG CAGGCAGAGG CTATGCTCAA GGAACGGGAC 
CAGGAACTGG AGGCCCTGCG GGCAGAGGGC CTGTCTTCCC AGCATCGGGA GGAGGCTGCC 
TGTGGCCAGG TGGAGGCTCT GCAGGAGGCC CTCAGCAAGG CTCAGGCTGA CCTGCAGGAG 
AAAGAGCAGC ATCTCCTTGG GCAGGTGGAA CTGAGCCGCA GCCTAGAGGC CAGCACGGCC 
ACGTTGCAGG CTGCCCTGGA CTCCTGCCAG GCACAGTCCC GGCAGCTGGA GGAGGCCCTG 
AGGAAGCGGG AAGGTGAGGT CCAGGTCCGG GACCTCCGAT ACCAGGAGGC CATGCAGCAG 
CTCCAGCAGG CACTTACCCA GAGGGATGAA GAGCTGAGAC AACAGAGGAA ACAGGGGCAG 
CTCCTGGAGA AGTCTCTGGC CCAGAGGGGC CAAGAGGATG CAACCCAAGA GAAGCAAGAA 
GCAAAGCACG AGAGAGAAGA GGAAGAGATA AGGAGCCTTC GTGAGAGTCT GCAGGAGCTA 
CAGCTGACTC TAGCACAAAA GGAAGAGGAG ATCCTGGAGC TGAGGGAGGC CCAGCAGAGG 
AAGAGTCTGG AGGACTTGTC TCACAAAGGC TGCCCAGGGA AGGAGCCGGC TACAACATTT 
GATTCCTTAG GGCCCAGGCT GCAGCGGGAA CTGGAGCGAC TACAGGCAGC ACTGAAGCAG 
ACAGAAGCCA GGGAGATCGA GTGGAGGGAG AAGGCCCAGG ATCTGGCGCT GTCCCTGGCC 
CAGAGCAAGG CCAGTGTCAG CGATCTGCAG GAGGTAGCCC TGTTCCTGCA AGCATCTGTT 
CTGGAGCGGG ACTCAGAACA GCAGAGGCTG CAGGATGAGT TGGAGCTCAC CAAACTCACT 
CTGGAGAAAG AGCGACTCCA CAGCCCAGGC CCGAGCAGCA GAGCAGAACG GGGGCCTAGA 
GGAGAGCCAG GTGTACAACT GGGAGAGGTC TCGGGAGTGA AGACTGAGCC TAGTCCTGAG 
ATGGAGGAGA GGCAGCTCTG GGAACAGAGG CTTGAGCACC TGCAGCGAGC GGTGGCTCTC 
TTGGAGGTTG ACCGAAGCCA GCTGCAACAA CATAACGCCC AGCTGCGGAC CACCCTGGAG 
CAGGTGGAGC GAGAGCGGAG GAAACTGAAG AGGGGTTCCA TGCGTGCATT GCGGGCAGGG 
GACCTGGAGA GCGGTGAAGC CACGGCACCA TCACCCATGC AACAGGATGG AAGAGGAAGG 
CAGAAGGGCT GCTCAGATGC CAAGCACATG GTTGAGCTGC AAAAAGAGGT GGCCCTCCTG 
CGAGCCCAGC TGGCCTTGGA GCGGAAGCAG AAGCAGGACT ACATTGCTCG CTCAGTTCAG 
ACGAGCCGTG AGCTGGCGGA CCTGCACCAC GGCCTCTCTC ACTCCCTTCT CGCTGTAGCC 
CAAGCCCCCG AGGCCACCGT CTTAGAGGCC GAGACCCGCA AGCTGGACGA GTCTCTGACT 
CAAAGCCTGA CATCCCCCGG GCCAGCACTG CTGTGCCCCA GCTCCAGTGC TGCTCAGACT 
GCCTCCAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013840764.1
CDS	192..7283
Translation	METGRPRLNNMKPQPLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQATLVRKLQAKVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_013985310.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013985310.1

ORF Insert Sequence:

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGG CACATCACCA GCAGGAGGCA GTCTCAGTTC AGATGGAGCA GCTGCGTCAG 
GAGGCAAAGC GGCAGGAAGA GGTGCTCGCC CGGGAAGTCC AGGAGAAGGA GGCTCTGGTG 
CGGGAAAAGG CGGCTCTTGA AGTGCGGCTG CAGGCTGTGG AGCGGGACCG GCAGGACCTC 
TCTGAACAAC TGCTGGGGCT CAGCTCGGCC AAGGAGCTTC TGGAGAACAG TCTGTTTGAG 
GCCCAGCAAC AGAATTCTCT GATAGAAGTC ACCAAGGGGC AGCTGGAGGT CCAGATTCAA 
ACTATCACTC AAGCCAAGGA GGTAATCCAA GGGGAAGTGA GGTGCCTACA GCTGGAACTG 
GATACTGAAC GGAGCCGGGC AGAACAGGAG CGGGATGCTG CAACCAAACA GCTGGCCCAG 
GCTGAGCAAG AGGGGCAGAC CGCCCTGCAG CAGCAGAAGT CAGCCCATGA GGAGGAAGTG 
AAGGGGCTCC AGGAAAAATG GGAGAAAGAG CGTGCCTGGC ACCAGCAGGA ACTGGATAAG 
GCTCTGAAGA GCCTTGAGAG GGAGAAAACG GAGCTGGAAA TGAGGCTGAG GGAGCAGCAG 
GCAGAAACAG AGGCCATGCG GACGCAGAGG GAAGAAGAAC GGGCTGAGGC TGAGAGCACC 
CTGCGCCAGA TGCAGCTCGA AACAGAGAAG GAGAGAATGT CCCTCCTGGA GACACTGCTG 
CAGACCCAGA AGGAGCTGGC AGATGCCAGC CAACAACTGG AGCGGCTGAG GCAGGACATG 
AAGGTTCAGA AGTTAAAGGA GCAGGAGACC ACTGGGATGC TGCAGACCCA GCTCCGGGAA 
GCTCAGAAGG AGCTGGAACA GGCAGCCCAG CAGCACAGAG ACAGCCTTGC TGCCCTCCAA 
GAAGAAGGCC GTGCCCTGCT GCAGGATAAG ATAGACCTGC ACAAGCAGGT GGAGGACTTG 
AAGTCTCAGC TGGTTTCCAA GGAAGACTCC CAAAGGCTGG TAGACCAGGA AGTTCAGGAG 
AAGCTGAGAG AGGCCCAGGA ATGTAGCCGA ATTCAGAAGG AGCTGGAGAA AGAAAAGGCC 
AGCCTGACTC TGTTGCTGGT GGAAAAGGAA CAGAGACTCC TTGGTTTACA AGAGGCCGAC 
TCCATTCGAC AACAAGAGCT GAGCTCCCTG CGCCAGGACA TGCAGGAGGC CCAGGCAGGG 
CAGAAAGAGC TCAGCGCCCA GGTGGAATTA CTGAAGCAGG AGGTGAAGGC AAAGGAGACT 
GACTTTCTGG CCCAGGAAGC GCGGCTGCTG GAAGAGCTGG AGGCATCTCA AGTAACAGAG 
CAGCAGCTAC GAGCTTCCTT GTGGGCCCAG GAAGCCAAGG CCGCACAACT ACAGCTGCGA 
CTGCGCAGCA CAGAGAGCCA GCTGGAGGTG CTGCTGGCAG AGCAGCAGCC TGGGCACCAG 
GCCCAGGCCC AGCTGGCCAG CCTTTGTGCT GTCCTGCAGC AGGCCCTGGG GTCTGTTTGT 
GAGAGCAGGC CTGAGCTGAG TGGCGGGGGA GACTCTGCTC CCTCCCTCTG GGGCCCTGAG 
CCAGACCAGA ATGGAGCTAG GACCCTTTTC AAGAGAGGGC CCCTCCTGAC AGCTCTCTCG 
GCTGAGGCGG TGGCCTCTGC CCTTCACAAG CTTAACCAAG ACCTGTGGAA GACTCAGCAG 
GCCCGGGATG ATCTGAGGGA TCAGGCCCAG AAGCTGGGAC AACGTCTTAC TGAAACAGAG 
GCCGAGAAGA GCCAGCTCCA CACAGAGTTG CAGGCTCTGC AGAGGCAGCT CTCCCAGAAC 
CAGGAAGAGA GATCCAAGTG GGAAGGAAAA CAGACCTCCT TAGAATCTGA GCTGGTGGAA 
CTGCATGAAA CTGTGACATC CTTACAGAGT CGCCTACGGC AAGCAGAGCT GCAGGGAATA 
GAAGCCCAGA ATGAGCAAGA GTTACTGCAG GCAGCCAAGG AGAACCTGAC AGCCCAGGTG 
GAACACCTCC AAGCAGCTGT TGCAGAAGCC AGGGCTCAGG CAAGCGCTGC CGAGGTCCTG 
GAGGAAGACC TCAAAACTGC ACGCTCAGCC CTGAAACTGA AAAGCAAGGA GGTGGAGAGT 
GAGCGTGAGC GAGCCCAGGC CCTACAGGAG CAGGGCGAGC TGAAGGTGGC CCAAGGGAAA 
GCTTTGCAGG AGAATTTGGC TCGCCTGACT CAGACCCTAT CTGAGAGAGA AGGGGAGGTG 
GAAACTTTGC AGGGAAAAAT CCAGGAGCTG GAGAAGCAAC GGGAAATGCA AAAGGCTGCT 
TTGGAGGTGC TGTCTTTGGA CCTCAAAAAG AGGAACCAAG AGGTGGATCT GCAGCAAGAA 
CAGATTCAGG AGCTGGAGGA GTGCAGATCG GTTTTAGAGC ATCTCCCCAT GGCAGTCCAG 
GAGCGAGAGC AGAAGCTGAC TCTGCAGAGG GAGCAGATCA AGGAGCTCGA GAAGGACCGA 
GAGACACAGA GGAACATCTT GGAGCATCAG CTTCTGGAAC TTGAGAAGAA GGCCCAAGTG 
ATTGAGTCCC AGAAAGGACA GATTCAGGAT CTGAAGAAGC AGCTGGTTAC TCTGGAATGC 
CTGGCCCTGG AACTAGAGGA AAACCATCAC AAAATAGAGT GCCAGCAAAA GACAATTGAG 
GAGCTGGAGG GCCAGAGGGA AATGCAGAGG GCAGCTCTGA CCCATCTGAC ACTGGACCTG 
GAGGAAAGGA GCCAGGAGCT GCAGGTACAG GGCAGCCAGA TCCGGGAGCT AGAGAGTCAC 
AACAGCCTCT TGGCAAGAGA GCTCCAGGAG AAGGACCAGG AGATGACGTC CCAGCAAGAC 
CAGACAGAGG AACTGCAGAG GCAGAAGGAG CATTTGACAC AGGACCTCAA GAGGCGGGAA 
CAGGAGATAG TGCTGCTGAA GGAGAGGATT CAGGTCTTGG AAGATCAGAG AACCCTACAG 
ACCAAGATCC TAGAGGAGGA CCTGGAGCAG ATCAAAATGT CCTTGAGGGA GCGAGGCCGG 
GACCTGGCCT CGCAGAGGCA GCTGATGCAG GAGCGGGCAG AGGAAGGGAA GGGCCAGAGT 
AAAGCACAGC GCGGGAGCCT GGAGCATATG AAGCTGATCC TGCGTGATAA GGAGAAGGAG 
GTGGAATGCC AGCAGGAGCA TATCCAGGAA CTGCAGGAGC ACAAGGCCCA GCTGGAGCAG 
CAGCTCCAGG GCTTACACAG GAAGATGGGG GAGACCAGCC TTCTCCTGAC CCAGCGGGAG 
CAGGAGATAG TGGCTCTGCA GCGGCACCTG CAGGAAGCCA GGGAACAGGG AGAGCTGAAA 
GAGCAGTCAC TTCAAGGTCA GCTGGAAGAG GCCCAGAGGG CGCTGGCCCG AAGGGACCAG 
GAGCTCAAGG CCCTGCAGCA GCAGCAGGCC CAAGGCCAGG AGGAAAATGT GATGGAAAAG 
GCGGGCTTAC TGCAGAGAGA GCTGGAGCAG GCCCACGTGA CACTGAAGGA GCGCCAGGCG 
GAGCTTGAGG ATTACAAGGA GCATGTGAGA AGGCTCCAGG AAGAGCTGGC AGTGGAGGGG 
CAGCGGGTAC AGGCCCTGGA GGAGGTGTTG AGTGACCTAA GGGCTGAATC TCGGGAACAA 
GAGAAGGCCC TGCTGGCCCT CCAGCAGCAG TGTGCCGAGC AGGCCCAGGA GCACGAGGCA 
GAGGCCAGGG CCCTGCAGGA CAGCTGGCTG CAGGCAGAGG CTATGCTCAA GGAACGGGAC 
CAGGAACTGG AGGCCCTGCG GGCAGAGGGC CTGTCTTCCC AGCATCGGGA GGAGGCTGCC 
TGTGGCCAGG TGGAGGCTCT GCAGGAGGCC CTCAGCAAGG CTCAGGCTGA CCTGCAGGAG 
AAAGAGCAGC ATCTCCTTGG GCAGGTGGAA CTGAGCCGCA GCCTAGAGGC CAGCACGGCC 
ACGTTGCAGG CTGCCCTGGA CTCCTGCCAG GCACAGTCCC GGCAGCTGGA GGAGGCCCTG 
AGGAAGCGGG AAGGTGAGGT CCAGGTCCGG GACCTCCGAT ACCAGGAGGC CATGCAGCAG 
CTCCAGCAGG CACTTACCCA GAGGGATGAA GAGCTGAGAC AACAGAGGAA ACAGGGGCAG 
CTCCTGGAGA AGTCTCTGGC CCAGAGGGGC CAAGAGGATG CAACCCAAGA GAAGCAAGAA 
GCAAAGCACG AGAGAGAAGA GGAAGAGATA AGGAGCCTTC GTGAGAGTCT GCAGGAGCTA 
CAGCTGACTC TAGCACAAAA GGAAGAGGAG ATCCTGGAGC TGAGGGAGGC CCAGCAGAGG 
AAGAGTCTGG AGGACTTGTC TCACAAAGGC TGCCCAGGGA AGGAGCCGGC TACAACATTT 
GATTCCTTAG GGCCCAGGCT GCAGCGGGAA CTGGAGCGAC TACAGGCAGC ACTGAAGCAG 
ACAGAAGCCA GGGAGATCGA GTGGAGGGAG AAGGCCCAGG ATCTGGCGCT GTCCCTGGCC 
CAGAGCAAGG CCAGTGTCAG CGATCTGCAG GAGGTAGCCC TGTTCCTGCA AGCATCTGTT 
CTGGAGCGGG ACTCAGAACA GCAGAGGCTG CAGGATGAGT TGGAGCTCAC CAAACTCACT 
CTGGAGAAAG AGCGACTCCA CAGCCCAGGC CCGAGCAGCA GAGCAGAACG GGGGCCTAGA 
GGAGAGCCAG GTGTACAACT GGGAGAGGTC TCGGGAGTGA AGACTGAGCC TAGTCCTGAG 
ATGGAGGAGA GGCAGCTCTG GGAACAGAGG CTTGAGCACC TGCAGCGAGC GGTGGCTCTC 
TTGGAGGTTG ACCGAAGCCA GCTGCAACAA CATAACGCCC AGCTGCGGAC CACCCTGGAG 
CAGGTGGAGC GAGAGCGGAG GAAACTGAAG AGGGGTTCCA TGCGTGCATT GCGGGCAGGG 
GACCTGGAGA GCGGTGAAGC CACGGCACCA TCACCCATGC AACAGGATGG AAGAGGAAGG 
CAGAAGGGCT GCTCAGATGC CAAGCACATG GTTGAGCTGC AAAAAGAGGT GGCCCTCCTG 
CGAGCCCAGC TGGCCTTGGA GCGGAAGCAG AAGCAGGACT ACATTGCTCG CTCAGTTCAG 
ACGAGCCGTG AGCTGGCGGA CCTGCACCAC GGCCTCTCTC ACTCCCTTCT CGCTGTAGCC 
CAAGCCCCCG AGGCCACCGT CTTAGAGGCC GAGACCCGCA AGCTGGACGA GTCTCTGACT 
CAAAGCCTGA CATCCCCCGG GCCAGCACTG CTGTGCCCCA GCTCCAGTGC TGCTCAGACT 
GCCTCCAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_013985312.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013840766.1 Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X7, mRNA.

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CloneID	OSe44859
Clone ID Related Accession (Same CDS sequence)	XM_013985312.1 , XM_021078257.1
Accession Version	XM_013985312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	centrosome-associated protein CEP250 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003612568.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATA AGGATCCATG GTATTTTCTG 
GGGCCAGAAG GATTTGGAGG AAGACACCGT ACACCAGCAC TCTTTTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013840766.1
CDS	192..7262
Translation	METGRPRLNNMKPQPLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQATLVRKLQAKVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDKDPWYFLGPEGFGGRHRTPALFC

Target ORF information:

RefSeq Version	XM_013985312.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013985312.1

ORF Insert Sequence:

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATA AGGATCCATG GTATTTTCTG 
GGGCCAGAAG GATTTGGAGG AAGACACCGT ACACCAGCAC TCTTTTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_021078257.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020933916.1 Sus scrofa centrosomal protein 250 (CEP250), transcript variant X6, mRNA.

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CloneID	OSe44859
Clone ID Related Accession (Same CDS sequence)	XM_013985312.1 , XM_021078257.1
Accession Version	XM_021078257.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7071bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	centrosome-associated protein CEP250 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010459.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATA AGGATCCATG GTATTTTCTG 
GGGCCAGAAG GATTTGGAGG AAGACACCGT ACACCAGCAC TCTTTTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020933916.1
CDS	1175..8245
Translation	METGRPRLNNMKPQPLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQATLVRKLQAKVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDKDPWYFLGPEGFGGRHRTPALFC

Target ORF information:

RefSeq Version	XM_021078257.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078257.1

ORF Insert Sequence:

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCAGAACA GTCTATTGTG 
GAGCTGTCAA GTTCTGAGAA CAGCCTGAAG GCTGAGGTAG CTGATCTTCG GGCTGCAGCT 
GTCAAGCTCA GTGCCTTAAA TGAGGCTTTG GCCTTAGATA AAGTTGGACT GAACCAGCAG 
CTTCTCCAGT TAGAACAGCA GAACCAGTCT GTGTGCAGCA GGATGGAGGC AGCAGAGCAG 
GCAAGAAGCG CTTTGCAGGT GGGCCTGGCA GAGGTGGAGA GGAGCAGGGA AGCGCTGTGG 
GAAAAGAACA CTTACCTGGA GGCTCAGCTG CAAAAAGCAG AGGCGGCCAG GGCTGAGCTG 
CAGGCAGATC TCAGGGGCGT CCAGGAAGAG AAGGAAGAAA TTCAAGAGAA ACTAAGCGAG 
GCACATCACC AGCAGGAGGC AGTCTCAGTT CAGATGGAGC AGCTGCGTCA GGAGGCAAAG 
CGGCAGGAAG AGGTGCTCGC CCGGGAAGTC CAGGAGAAGG AGGCTCTGGT GCGGGAAAAG 
GCGGCTCTTG AAGTGCGGCT GCAGGCTGTG GAGCGGGACC GGCAGGACCT CTCTGAACAA 
CTGCTGGGGC TCAGCTCGGC CAAGGAGCTT CTGGAGAACA GTCTGTTTGA GGCCCAGCAA 
CAGAATTCTC TGATAGAAGT CACCAAGGGG CAGCTGGAGG TCCAGATTCA AACTATCACT 
CAAGCCAAGG AGGTAATCCA AGGGGAAGTG AGGTGCCTAC AGCTGGAACT GGATACTGAA 
CGGAGCCGGG CAGAACAGGA GCGGGATGCT GCAACCAAAC AGCTGGCCCA GGCTGAGCAA 
GAGGGGCAGA CCGCCCTGCA GCAGCAGAAG TCAGCCCATG AGGAGGAAGT GAAGGGGCTC 
CAGGAAAAAT GGGAGAAAGA GCGTGCCTGG CACCAGCAGG AACTGGATAA GGCTCTGAAG 
AGCCTTGAGA GGGAGAAAAC GGAGCTGGAA ATGAGGCTGA GGGAGCAGCA GGCAGAAACA 
GAGGCCATGC GGACGCAGAG GGAAGAAGAA CGGGCTGAGG CTGAGAGCAC CCTGCGCCAG 
ATGCAGCTCG AAACAGAGAA GGAGAGAATG TCCCTCCTGG AGACACTGCT GCAGACCCAG 
AAGGAGCTGG CAGATGCCAG CCAACAACTG GAGCGGCTGA GGCAGGACAT GAAGGTTCAG 
AAGTTAAAGG AGCAGGAGAC CACTGGGATG CTGCAGACCC AGCTCCGGGA AGCTCAGAAG 
GAGCTGGAAC AGGCAGCCCA GCAGCACAGA GACAGCCTTG CTGCCCTCCA AGAAGAAGGC 
CGTGCCCTGC TGCAGGATAA GATAGACCTG CACAAGCAGG TGGAGGACTT GAAGTCTCAG 
CTGGTTTCCA AGGAAGACTC CCAAAGGCTG GTAGACCAGG AAGTTCAGGA GAAGCTGAGA 
GAGGCCCAGG AATGTAGCCG AATTCAGAAG GAGCTGGAGA AAGAAAAGGC CAGCCTGACT 
CTGTTGCTGG TGGAAAAGGA ACAGAGACTC CTTGGTTTAC AAGAGGCCGA CTCCATTCGA 
CAACAAGAGC TGAGCTCCCT GCGCCAGGAC ATGCAGGAGG CCCAGGCAGG GCAGAAAGAG 
CTCAGCGCCC AGGTGGAATT ACTGAAGCAG GAGGTGAAGG CAAAGGAGAC TGACTTTCTG 
GCCCAGGAAG CGCGGCTGCT GGAAGAGCTG GAGGCATCTC AAGTAACAGA GCAGCAGCTA 
CGAGCTTCCT TGTGGGCCCA GGAAGCCAAG GCCGCACAAC TACAGCTGCG ACTGCGCAGC 
ACAGAGAGCC AGCTGGAGGT GCTGCTGGCA GAGCAGCAGC CTGGGCACCA GGCCCAGGCC 
CAGCTGGCCA GCCTTTGTGC TGTCCTGCAG CAGGCCCTGG GGTCTGTTTG TGAGAGCAGG 
CCTGAGCTGA GTGGCGGGGG AGACTCTGCT CCCTCCCTCT GGGGCCCTGA GCCAGACCAG 
AATGGAGCTA GGACCCTTTT CAAGAGAGGG CCCCTCCTGA CAGCTCTCTC GGCTGAGGCG 
GTGGCCTCTG CCCTTCACAA GCTTAACCAA GACCTGTGGA AGACTCAGCA GGCCCGGGAT 
GATCTGAGGG ATCAGGCCCA GAAGCTGGGA CAACGTCTTA CTGAAACAGA GGCCGAGAAG 
AGCCAGCTCC ACACAGAGTT GCAGGCTCTG CAGAGGCAGC TCTCCCAGAA CCAGGAAGAG 
AGATCCAAGT GGGAAGGAAA ACAGACCTCC TTAGAATCTG AGCTGGTGGA ACTGCATGAA 
ACTGTGACAT CCTTACAGAG TCGCCTACGG CAAGCAGAGC TGCAGGGAAT AGAAGCCCAG 
AATGAGCAAG AGTTACTGCA GGCAGCCAAG GAGAACCTGA CAGCCCAGGT GGAACACCTC 
CAAGCAGCTG TTGCAGAAGC CAGGGCTCAG GCAAGCGCTG CCGAGGTCCT GGAGGAAGAC 
CTCAAAACTG CACGCTCAGC CCTGAAACTG AAAAGCAAGG AGGTGGAGAG TGAGCGTGAG 
CGAGCCCAGG CCCTACAGGA GCAGGGCGAG CTGAAGGTGG CCCAAGGGAA AGCTTTGCAG 
GAGAATTTGG CTCGCCTGAC TCAGACCCTA TCTGAGAGAG AAGGGGAGGT GGAAACTTTG 
CAGGGAAAAA TCCAGGAGCT GGAGAAGCAA CGGGAAATGC AAAAGGCTGC TTTGGAGGTG 
CTGTCTTTGG ACCTCAAAAA GAGGAACCAA GAGGTGGATC TGCAGCAAGA ACAGATTCAG 
GAGCTGGAGG AGTGCAGATC GGTTTTAGAG CATCTCCCCA TGGCAGTCCA GGAGCGAGAG 
CAGAAGCTGA CTCTGCAGAG GGAGCAGATC AAGGAGCTCG AGAAGGACCG AGAGACACAG 
AGGAACATCT TGGAGCATCA GCTTCTGGAA CTTGAGAAGA AGGCCCAAGT GATTGAGTCC 
CAGAAAGGAC AGATTCAGGA TCTGAAGAAG CAGCTGGTTA CTCTGGAATG CCTGGCCCTG 
GAACTAGAGG AAAACCATCA CAAAATAGAG TGCCAGCAAA AGACAATTGA GGAGCTGGAG 
GGCCAGAGGG AAATGCAGAG GGCAGCTCTG ACCCATCTGA CACTGGACCT GGAGGAAAGG 
AGCCAGGAGC TGCAGGTACA GGGCAGCCAG ATCCGGGAGC TAGAGAGTCA CAACAGCCTC 
TTGGCAAGAG AGCTCCAGGA GAAGGACCAG GAGATGACGT CCCAGCAAGA CCAGACAGAG 
GAACTGCAGA GGCAGAAGGA GCATTTGACA CAGGACCTCA AGAGGCGGGA ACAGGAGATA 
GTGCTGCTGA AGGAGAGGAT TCAGGTCTTG GAAGATCAGA GAACCCTACA GACCAAGATC 
CTAGAGGAGG ACCTGGAGCA GATCAAAATG TCCTTGAGGG AGCGAGGCCG GGACCTGGCC 
TCGCAGAGGC AGCTGATGCA GGAGCGGGCA GAGGAAGGGA AGGGCCAGAG TAAAGCACAG 
CGCGGGAGCC TGGAGCATAT GAAGCTGATC CTGCGTGATA AGGAGAAGGA GGTGGAATGC 
CAGCAGGAGC ATATCCAGGA ACTGCAGGAG CACAAGGCCC AGCTGGAGCA GCAGCTCCAG 
GGCTTACACA GGAAGATGGG GGAGACCAGC CTTCTCCTGA CCCAGCGGGA GCAGGAGATA 
GTGGCTCTGC AGCGGCACCT GCAGGAAGCC AGGGAACAGG GAGAGCTGAA AGAGCAGTCA 
CTTCAAGGTC AGCTGGAAGA GGCCCAGAGG GCGCTGGCCC GAAGGGACCA GGAGCTCAAG 
GCCCTGCAGC AGCAGCAGGC CCAAGGCCAG GAGGAAAATG TGATGGAAAA GGCGGGCTTA 
CTGCAGAGAG AGCTGGAGCA GGCCCACGTG ACACTGAAGG AGCGCCAGGC GGAGCTTGAG 
GATTACAAGG AGCATGTGAG AAGGCTCCAG GAAGAGCTGG CAGTGGAGGG GCAGCGGGTA 
CAGGCCCTGG AGGAGGTGTT GAGTGACCTA AGGGCTGAAT CTCGGGAACA AGAGAAGGCC 
CTGCTGGCCC TCCAGCAGCA GTGTGCCGAG CAGGCCCAGG AGCACGAGGC AGAGGCCAGG 
GCCCTGCAGG ACAGCTGGCT GCAGGCAGAG GCTATGCTCA AGGAACGGGA CCAGGAACTG 
GAGGCCCTGC GGGCAGAGGG CCTGTCTTCC CAGCATCGGG AGGAGGCTGC CTGTGGCCAG 
GTGGAGGCTC TGCAGGAGGC CCTCAGCAAG GCTCAGGCTG ACCTGCAGGA GAAAGAGCAG 
CATCTCCTTG GGCAGGTGGA ACTGAGCCGC AGCCTAGAGG CCAGCACGGC CACGTTGCAG 
GCTGCCCTGG ACTCCTGCCA GGCACAGTCC CGGCAGCTGG AGGAGGCCCT GAGGAAGCGG 
GAAGGTGAGG TCCAGGTCCG GGACCTCCGA TACCAGGAGG CCATGCAGCA GCTCCAGCAG 
GCACTTACCC AGAGGGATGA AGAGCTGAGA CAACAGAGGA AACAGGGGCA GCTCCTGGAG 
AAGTCTCTGG CCCAGAGGGG CCAAGAGGAT GCAACCCAAG AGAAGCAAGA AGCAAAGCAC 
GAGAGAGAAG AGGAAGAGAT AAGGAGCCTT CGTGAGAGTC TGCAGGAGCT ACAGCTGACT 
CTAGCACAAA AGGAAGAGGA GATCCTGGAG CTGAGGGAGG CCCAGCAGAG GAAGAGTCTG 
GAGGACTTGT CTCACAAAGG CTGCCCAGGG AAGGAGCCGG CTACAACATT TGATTCCTTA 
GGGCCCAGGC TGCAGCGGGA ACTGGAGCGA CTACAGGCAG CACTGAAGCA GACAGAAGCC 
AGGGAGATCG AGTGGAGGGA GAAGGCCCAG GATCTGGCGC TGTCCCTGGC CCAGAGCAAG 
GCCAGTGTCA GCGATCTGCA GGAGGTAGCC CTGTTCCTGC AAGCATCTGT TCTGGAGCGG 
GACTCAGAAC AGCAGAGGCT GCAGGATGAG TTGGAGCTCA CCAAACTCAC TCTGGAGAAA 
GAGCGACTCC ACAGCCCAGG CCCGAGCAGC AGAGCAGAAC GGGGGCCTAG AGGAGAGCCA 
GGTGTACAAC TGGGAGAGGT CTCGGGAGTG AAGACTGAGC CTAGTCCTGA GATGGAGGAG 
AGGCAGCTCT GGGAACAGAG GCTTGAGCAC CTGCAGCGAG CGGTGGCTCT CTTGGAGGTT 
GACCGAAGCC AGCTGCAACA ACATAACGCC CAGCTGCGGA CCACCCTGGA GCAGGTGGAG 
CGAGAGCGGA GGAAACTGAA GAGGGGTTCC ATGCGTGCAT TGCGGGCAGG GGACCTGGAG 
AGCGGTGAAG CCACGGCACC ATCACCCATG CAACAGGATA AGGATCCATG GTATTTTCTG 
GGGCCAGAAG GATTTGGAGG AAGACACCGT ACACCAGCAC TCTTTTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_013985313.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013840767.1 Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X8, mRNA.

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CloneID	OSe44860
Clone ID Related Accession (Same CDS sequence)	XM_013985313.1
Accession Version	XM_013985313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	centrosome-associated protein CEP250 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003612568.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCACATCA CCAGCAGGAG 
GCAGTCTCAG TTCAGATGGA GCAGCTGCGT CAGGAGGCAA AGCGGCAGGA AGAGGTGCTC 
GCCCGGGAAG TCCAGGAGAA GGAGGCTCTG GTGCGGGAAA AGGCGGCTCT TGAAGTGCGG 
CTGCAGGCTG TGGAGCGGGA CCGGCAGGAC CTCTCTGAAC AACTGCTGGG GCTCAGCTCG 
GCCAAGGAGC TTCTGGAGAA CAGTCTGTTT GAGGCCCAGC AACAGAATTC TCTGATAGAA 
GTCACCAAGG GGCAGCTGGA GGTCCAGATT CAAACTATCA CTCAAGCCAA GGAGGTAATC 
CAAGGGGAAG TGAGGTGCCT ACAGCTGGAA CTGGATACTG AACGGAGCCG GGCAGAACAG 
GAGCGGGATG CTGCAACCAA ACAGCTGGCC CAGGCTGAGC AAGAGGGGCA GACCGCCCTG 
CAGCAGCAGA AGTCAGCCCA TGAGGAGGAA GTGAAGGGGC TCCAGGAAAA ATGGGAGAAA 
GAGCGTGCCT GGCACCAGCA GGAACTGGAT AAGGCTCTGA AGAGCCTTGA GAGGGAGAAA 
ACGGAGCTGG AAATGAGGCT GAGGGAGCAG CAGGCAGAAA CAGAGGCCAT GCGGACGCAG 
AGGGAAGAAG AACGGGCTGA GGCTGAGAGC ACCCTGCGCC AGATGCAGCT CGAAACAGAG 
AAGGAGAGAA TGTCCCTCCT GGAGACACTG CTGCAGACCC AGAAGGAGCT GGCAGATGCC 
AGCCAACAAC TGGAGCGGCT GAGGCAGGAC ATGAAGGTTC AGAAGTTAAA GGAGCAGGAG 
ACCACTGGGA TGCTGCAGAC CCAGCTCCGG GAAGCTCAGA AGGAGCTGGA ACAGGCAGCC 
CAGCAGCACA GAGACAGCCT TGCTGCCCTC CAAGAAGAAG GCCGTGCCCT GCTGCAGGAT 
AAGATAGACC TGCACAAGCA GGTGGAGGAC TTGAAGTCTC AGCTGGTTTC CAAGGAAGAC 
TCCCAAAGGC TGGTAGACCA GGAAGTTCAG GAGAAGCTGA GAGAGGCCCA GGAATGTAGC 
CGAATTCAGA AGGAGCTGGA GAAAGAAAAG GCCAGCCTGA CTCTGTTGCT GGTGGAAAAG 
GAACAGAGAC TCCTTGGTTT ACAAGAGGCC GACTCCATTC GACAACAAGA GCTGAGCTCC 
CTGCGCCAGG ACATGCAGGA GGCCCAGGCA GGGCAGAAAG AGCTCAGCGC CCAGGTGGAA 
TTACTGAAGC AGGAGGTGAA GGCAAAGGAG ACTGACTTTC TGGCCCAGGA AGCGCGGCTG 
CTGGAAGAGC TGGAGGCATC TCAAGTAACA GAGCAGCAGC TACGAGCTTC CTTGTGGGCC 
CAGGAAGCCA AGGCCGCACA ACTACAGCTG CGACTGCGCA GCACAGAGAG CCAGCTGGAG 
GTGCTGCTGG CAGAGCAGCA GCCTGGGCAC CAGGCCCAGG CCCAGCTGGC CAGCCTTTGT 
GCTGTCCTGC AGCAGGCCCT GGGGTCTGTT TGTGAGAGCA GGCCTGAGCT GAGTGGCGGG 
GGAGACTCTG CTCCCTCCCT CTGGGGCCCT GAGCCAGACC AGAATGGAGC TAGGACCCTT 
TTCAAGAGAG GGCCCCTCCT GACAGCTCTC TCGGCTGAGG CGGTGGCCTC TGCCCTTCAC 
AAGCTTAACC AAGACCTGTG GAAGACTCAG CAGGCCCGGG ATGATCTGAG GGATCAGGCC 
CAGAAGCTGG GACAACGTCT TACTGAAACA GAGGCCGAGA AGAGCCAGCT CCACACAGAG 
TTGCAGGCTC TGCAGAGGCA GCTCTCCCAG AACCAGGAAG AGAGATCCAA GTGGGAAGGA 
AAACAGACCT CCTTAGAATC TGAGCTGGTG GAACTGCATG AAACTGTGAC ATCCTTACAG 
AGTCGCCTAC GGCAAGCAGA GCTGCAGGGA ATAGAAGCCC AGAATGAGCA AGAGTTACTG 
CAGGCAGCCA AGGAGAACCT GACAGCCCAG GTGGAACACC TCCAAGCAGC TGTTGCAGAA 
GCCAGGGCTC AGGCAAGCGC TGCCGAGGTC CTGGAGGAAG ACCTCAAAAC TGCACGCTCA 
GCCCTGAAAC TGAAAAGCAA GGAGGTGGAG AGTGAGCGTG AGCGAGCCCA GGCCCTACAG 
GAGCAGGGCG AGCTGAAGGT GGCCCAAGGG AAAGCTTTGC AGGAGAATTT GGCTCGCCTG 
ACTCAGACCC TATCTGAGAG AGAAGGGGAG GTGGAAACTT TGCAGGGAAA AATCCAGGAG 
CTGGAGAAGC AACGGGAAAT GCAAAAGGCT GCTTTGGAGG TGCTGTCTTT GGACCTCAAA 
AAGAGGAACC AAGAGGTGGA TCTGCAGCAA GAACAGATTC AGGAGCTGGA GGAGTGCAGA 
TCGGTTTTAG AGCATCTCCC CATGGCAGTC CAGGAGCGAG AGCAGAAGCT GACTCTGCAG 
AGGGAGCAGA TCAAGGAGCT CGAGAAGGAC CGAGAGACAC AGAGGAACAT CTTGGAGCAT 
CAGCTTCTGG AACTTGAGAA GAAGGCCCAA GTGATTGAGT CCCAGAAAGG ACAGATTCAG 
GATCTGAAGA AGCAGCTGGT TACTCTGGAA TGCCTGGCCC TGGAACTAGA GGAAAACCAT 
CACAAAATAG AGTGCCAGCA AAAGACAATT GAGGAGCTGG AGGGCCAGAG GGAAATGCAG 
AGGGCAGCTC TGACCCATCT GACACTGGAC CTGGAGGAAA GGAGCCAGGA GCTGCAGGTA 
CAGGGCAGCC AGATCCGGGA GCTAGAGAGT CACAACAGCC TCTTGGCAAG AGAGCTCCAG 
GAGAAGGACC AGGAGATGAC GTCCCAGCAA GACCAGACAG AGGAACTGCA GAGGCAGAAG 
GAGCATTTGA CACAGGACCT CAAGAGGCGG GAACAGGAGA TAGTGCTGCT GAAGGAGAGG 
ATTCAGGTCT TGGAAGATCA GAGAACCCTA CAGACCAAGA TCCTAGAGGA GGACCTGGAG 
CAGATCAAAA TGTCCTTGAG GGAGCGAGGC CGGGACCTGG CCTCGCAGAG GCAGCTGATG 
CAGGAGCGGG CAGAGGAAGG GAAGGGCCAG AGTAAAGCAC AGCGCGGGAG CCTGGAGCAT 
ATGAAGCTGA TCCTGCGTGA TAAGGAGAAG GAGGTGGAAT GCCAGCAGGA GCATATCCAG 
GAACTGCAGG AGCACAAGGC CCAGCTGGAG CAGCAGCTCC AGGGCTTACA CAGGAAGATG 
GGGGAGACCA GCCTTCTCCT GACCCAGCGG GAGCAGGAGA TAGTGGCTCT GCAGCGGCAC 
CTGCAGGAAG CCAGGGAACA GGGAGAGCTG AAAGAGCAGT CACTTCAAGG TCAGCTGGAA 
GAGGCCCAGA GGGCGCTGGC CCGAAGGGAC CAGGAGCTCA AGGCCCTGCA GCAGCAGCAG 
GCCCAAGGCC AGGAGGAAAA TGTGATGGAA AAGGCGGGCT TACTGCAGAG AGAGCTGGAG 
CAGGCCCACG TGACACTGAA GGAGCGCCAG GCGGAGCTTG AGGATTACAA GGAGCATGTG 
AGAAGGCTCC AGGAAGAGCT GGCAGTGGAG GGGCAGCGGG TACAGGCCCT GGAGGAGGTG 
TTGAGTGACC TAAGGGCTGA ATCTCGGGAA CAAGAGAAGG CCCTGCTGGC CCTCCAGCAG 
CAGTGTGCCG AGCAGGCCCA GGAGCACGAG GCAGAGGCCA GGGCCCTGCA GGACAGCTGG 
CTGCAGGCAG AGGCTATGCT CAAGGAACGG GACCAGGAAC TGGAGGCCCT GCGGGCAGAG 
GGCCTGTCTT CCCAGCATCG GGAGGAGGCT GCCTGTGGCC AGGTGGAGGC TCTGCAGGAG 
GCCCTCAGCA AGGCTCAGGC TGACCTGCAG GAGAAAGAGC AGCATCTCCT TGGGCAGGTG 
GAACTGAGCC GCAGCCTAGA GGCCAGCACG GCCACGTTGC AGGCTGCCCT GGACTCCTGC 
CAGGCACAGT CCCGGCAGCT GGAGGAGGCC CTGAGGAAGC GGGAAGGTGA GGTCCAGGTC 
CGGGACCTCC GATACCAGGA GGCCATGCAG CAGCTCCAGC AGGCACTTAC CCAGAGGGAT 
GAAGAGCTGA GACAACAGAG GAAACAGGGG CAGCTCCTGG AGAAGTCTCT GGCCCAGAGG 
GGCCAAGAGG ATGCAACCCA AGAGAAGCAA GAAGCAAAGC ACGAGAGAGA AGAGGAAGAG 
ATAAGGAGCC TTCGTGAGAG TCTGCAGGAG CTACAGCTGA CTCTAGCACA AAAGGAAGAG 
GAGATCCTGG AGCTGAGGGA GGCCCAGCAG AGGAAGAGTC TGGAGGACTT GTCTCACAAA 
GGCTGCCCAG GGAAGGAGCC GGCTACAACA TTTGATTCCT TAGGGCCCAG GCTGCAGCGG 
GAACTGGAGC GACTACAGGC AGCACTGAAG CAGACAGAAG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGGATCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGTGT CAGCGATCTG 
CAGGAGGTAG CCCTGTTCCT GCAAGCATCT GTTCTGGAGC GGGACTCAGA ACAGCAGAGG 
CTGCAGGATG AGTTGGAGCT CACCAAACTC ACTCTGGAGA AAGAGCGACT CCACAGCCCA 
GGCCCGAGCA GCAGAGCAGA ACGGGGGCCT AGAGGAGAGC CAGGTGTACA ACTGGGAGAG 
GTCTCGGGAG TGAAGACTGA GCCTAGTCCT GAGATGGAGG AGAGGCAGCT CTGGGAACAG 
AGGCTTGAGC ACCTGCAGCG AGCGGTGGCT CTCTTGGAGG TTGACCGAAG CCAGCTGCAA 
CAACATAACG CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGGTT CCATGCGTGC ATTGCGGGCA GGGGACCTGG AGAGCGGTGA AGCCACGGCA 
CCATCACCCA TGCAACAGGA TGGAAGAGGA AGGCAGAAGG GCTGCTCAGA TGCCAAGCAC 
ATGGTTGAGC TGCAAAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATTGC TCGCTCAGTT CAGACGAGCC GTGAGCTGGC GGACCTGCAC 
CACGGCCTCT CTCACTCCCT TCTCGCTGTA GCCCAAGCCC CCGAGGCCAC CGTCTTAGAG 
GCCGAGACCC GCAAGCTGGA CGAGTCTCTG ACTCAAAGCC TGACATCCCC CGGGCCAGCA 
CTGCTGTGCC CCAGCTCCAG TGCTGCTCAG ACTGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013840767.1
CDS	192..7136
Translation	METGRPRLNNMKPQPLQLVLEEQVLALQQQMAENQAASWRKLKSSQEAQQRQATLVRKLQAKVLQYRSWSQELEKRLEATGAPIPQRWENVEEPNLEQLLIRLEEEQQRCESLAEVNTQLRLHMEKADVVNKALREDVEKLTVDWSQARDELMRKESQWQMEQEFFKGYLKGEHGRLLSLWREVVTFRRHFLEMKSATDRDLMELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_013985313.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013985313.1

ORF Insert Sequence:

ATGGAGACAG GCCGCCCCAG GCTGAACAAC ATGAAGCCCC AACCACTGCA GCTGGTGCTA 
GAGGAGCAAG TGCTGGCGCT GCAGCAGCAG ATGGCAGAGA ACCAAGCAGC CTCCTGGCGG 
AAGCTGAAGA GCTCCCAAGA GGCACAGCAG AGGCAAGCAA CCCTTGTGAG GAAGCTGCAG 
GCCAAGGTGT TGCAGTACCG GAGCTGGAGC CAAGAGCTGG AGAAGCGGCT GGAAGCCACT 
GGGGCACCAA TCCCTCAGAG GTGGGAAAAT GTGGAGGAGC CAAACCTGGA ACAGCTGCTA 
ATCCGATTGG AGGAGGAGCA ACAGAGGTGT GAGAGTCTAG CAGAGGTGAA CACCCAGCTT 
CGGCTGCACA TGGAAAAAGC TGACGTGGTG AATAAAGCGC TTCGGGAAGA TGTGGAAAAA 
TTGACAGTAG ACTGGAGCCA GGCCCGGGAT GAGCTAATGA GGAAGGAGAG CCAGTGGCAG 
ATGGAGCAGG AGTTCTTCAA GGGCTACCTG AAAGGGGAGC ATGGTCGCCT TCTCAGTCTA 
TGGCGGGAGG TGGTGACCTT CCGACGTCAC TTTCTGGAAA TGAAGTCAGC CACTGACAGA 
GACCTGATGG AGCTCAAGGC TGAGCACGTG AAGCTCTCTG GGTCCCTGTT GACCTGTTGT 
CTGCGCTTGA CTGTGGGAGC ACCACAGGCC CGGGAATCCA CTGGATCTGG GAGGCTGAAT 
GGGAGTGAAC CATCCCAGCT GATGCTGCTA CTGTCTAAGA CCCAGGAGCT GGAGAAGGAA 
GCCCATAAAA GGAGCCAGGA GTTAATACAG CTTAAGAGTC AAGGGGATCT GGAGAAGGCT 
GAGCTTCAGG ATCGGGTGAC CGAGCTTTCT GCTCTGCTGA CCCAGTCTCA GAAGCAGAAT 
GAAGATTATG AAAAGATGGT AAAGGCTCTG AGAGAGACAA TGAAGATCCT GGAGGCAAAT 
CATGCAGAGT TAATGGAACA TGAGGCATCT CTTAGTAGGA ATGCCCAAGA GGAGAGGCTG 
TCTTTACAGC AAGTGATCAA GGATATAACC CAGGCCATGG TAGAAGAAGG GGACCATATG 
GCCCAAGGCT CTGGTCACGA GCACTCCTTG GACTTGGATC CTAGTGGCTT CTTCTCCCAG 
TTTGATTCCC AGCAGCCAGA CAAGGCCCTC ACTCTGGTGC GTTCTGTGTT GACTCAGAGA 
CGCCAGGCTG TGCAGGACCT AAAGCAGCAG CTTTCAGGCT GTCAGGAAGC TGTGAGCTCC 
TTGCAGCAGC AGCATCATCA GTGGGAAGAA GAGGGTGAGG CCTTGAGACA GCGGCTGCAG 
AAGCTCACCG GGGAGCGGGA CACCCTGGCA GGGCAGACTG TGGGCCTGCA GGGGGAGGTG 
GACTCTCTGA GCAGGGAGCG AGAGCTCCTG CAAAAGACCA GGGAGGAGCT GCAGCAGCAG 
CTGGAGGTGC TGGAGCAGGA GGCCTGGCGA CTACGGAGGA CAAACATGGA GCTTCAGCTG 
CAGGGCGACT CTGCTCAGGG CGAGAAGGAG GAGCAGCAGG AGGAGCTGCA CCTGGCTGTC 
CGAGAACGGG AGCGCCTTCA GGAGATGCTG GCGAGCCTGG AAGCCAAACA GTCAGAATCT 
CTCAGTGAAC TGATCACCCT TCGGGAAGCC CTGGAGTCTA GTCGCCTAGA AGGGGAGTTG 
CTGAGGCAAG AGCAAACAGA AGTGACTGCT GTGCTAGCAA AGGCACATCA CCAGCAGGAG 
GCAGTCTCAG TTCAGATGGA GCAGCTGCGT CAGGAGGCAA AGCGGCAGGA AGAGGTGCTC 
GCCCGGGAAG TCCAGGAGAA GGAGGCTCTG GTGCGGGAAA AGGCGGCTCT TGAAGTGCGG 
CTGCAGGCTG TGGAGCGGGA CCGGCAGGAC CTCTCTGAAC AACTGCTGGG GCTCAGCTCG 
GCCAAGGAGC TTCTGGAGAA CAGTCTGTTT GAGGCCCAGC AACAGAATTC TCTGATAGAA 
GTCACCAAGG GGCAGCTGGA GGTCCAGATT CAAACTATCA CTCAAGCCAA GGAGGTAATC 
CAAGGGGAAG TGAGGTGCCT ACAGCTGGAA CTGGATACTG AACGGAGCCG GGCAGAACAG 
GAGCGGGATG CTGCAACCAA ACAGCTGGCC CAGGCTGAGC AAGAGGGGCA GACCGCCCTG 
CAGCAGCAGA AGTCAGCCCA TGAGGAGGAA GTGAAGGGGC TCCAGGAAAA ATGGGAGAAA 
GAGCGTGCCT GGCACCAGCA GGAACTGGAT AAGGCTCTGA AGAGCCTTGA GAGGGAGAAA 
ACGGAGCTGG AAATGAGGCT GAGGGAGCAG CAGGCAGAAA CAGAGGCCAT GCGGACGCAG 
AGGGAAGAAG AACGGGCTGA GGCTGAGAGC ACCCTGCGCC AGATGCAGCT CGAAACAGAG 
AAGGAGAGAA TGTCCCTCCT GGAGACACTG CTGCAGACCC AGAAGGAGCT GGCAGATGCC 
AGCCAACAAC TGGAGCGGCT GAGGCAGGAC ATGAAGGTTC AGAAGTTAAA GGAGCAGGAG 
ACCACTGGGA TGCTGCAGAC CCAGCTCCGG GAAGCTCAGA AGGAGCTGGA ACAGGCAGCC 
CAGCAGCACA GAGACAGCCT TGCTGCCCTC CAAGAAGAAG GCCGTGCCCT GCTGCAGGAT 
AAGATAGACC TGCACAAGCA GGTGGAGGAC TTGAAGTCTC AGCTGGTTTC CAAGGAAGAC 
TCCCAAAGGC TGGTAGACCA GGAAGTTCAG GAGAAGCTGA GAGAGGCCCA GGAATGTAGC 
CGAATTCAGA AGGAGCTGGA GAAAGAAAAG GCCAGCCTGA CTCTGTTGCT GGTGGAAAAG 
GAACAGAGAC TCCTTGGTTT ACAAGAGGCC GACTCCATTC GACAACAAGA GCTGAGCTCC 
CTGCGCCAGG ACATGCAGGA GGCCCAGGCA GGGCAGAAAG AGCTCAGCGC CCAGGTGGAA 
TTACTGAAGC AGGAGGTGAA GGCAAAGGAG ACTGACTTTC TGGCCCAGGA AGCGCGGCTG 
CTGGAAGAGC TGGAGGCATC TCAAGTAACA GAGCAGCAGC TACGAGCTTC CTTGTGGGCC 
CAGGAAGCCA AGGCCGCACA ACTACAGCTG CGACTGCGCA GCACAGAGAG CCAGCTGGAG 
GTGCTGCTGG CAGAGCAGCA GCCTGGGCAC CAGGCCCAGG CCCAGCTGGC CAGCCTTTGT 
GCTGTCCTGC AGCAGGCCCT GGGGTCTGTT TGTGAGAGCA GGCCTGAGCT GAGTGGCGGG 
GGAGACTCTG CTCCCTCCCT CTGGGGCCCT GAGCCAGACC AGAATGGAGC TAGGACCCTT 
TTCAAGAGAG GGCCCCTCCT GACAGCTCTC TCGGCTGAGG CGGTGGCCTC TGCCCTTCAC 
AAGCTTAACC AAGACCTGTG GAAGACTCAG CAGGCCCGGG ATGATCTGAG GGATCAGGCC 
CAGAAGCTGG GACAACGTCT TACTGAAACA GAGGCCGAGA AGAGCCAGCT CCACACAGAG 
TTGCAGGCTC TGCAGAGGCA GCTCTCCCAG AACCAGGAAG AGAGATCCAA GTGGGAAGGA 
AAACAGACCT CCTTAGAATC TGAGCTGGTG GAACTGCATG AAACTGTGAC ATCCTTACAG 
AGTCGCCTAC GGCAAGCAGA GCTGCAGGGA ATAGAAGCCC AGAATGAGCA AGAGTTACTG 
CAGGCAGCCA AGGAGAACCT GACAGCCCAG GTGGAACACC TCCAAGCAGC TGTTGCAGAA 
GCCAGGGCTC AGGCAAGCGC TGCCGAGGTC CTGGAGGAAG ACCTCAAAAC TGCACGCTCA 
GCCCTGAAAC TGAAAAGCAA GGAGGTGGAG AGTGAGCGTG AGCGAGCCCA GGCCCTACAG 
GAGCAGGGCG AGCTGAAGGT GGCCCAAGGG AAAGCTTTGC AGGAGAATTT GGCTCGCCTG 
ACTCAGACCC TATCTGAGAG AGAAGGGGAG GTGGAAACTT TGCAGGGAAA AATCCAGGAG 
CTGGAGAAGC AACGGGAAAT GCAAAAGGCT GCTTTGGAGG TGCTGTCTTT GGACCTCAAA 
AAGAGGAACC AAGAGGTGGA TCTGCAGCAA GAACAGATTC AGGAGCTGGA GGAGTGCAGA 
TCGGTTTTAG AGCATCTCCC CATGGCAGTC CAGGAGCGAG AGCAGAAGCT GACTCTGCAG 
AGGGAGCAGA TCAAGGAGCT CGAGAAGGAC CGAGAGACAC AGAGGAACAT CTTGGAGCAT 
CAGCTTCTGG AACTTGAGAA GAAGGCCCAA GTGATTGAGT CCCAGAAAGG ACAGATTCAG 
GATCTGAAGA AGCAGCTGGT TACTCTGGAA TGCCTGGCCC TGGAACTAGA GGAAAACCAT 
CACAAAATAG AGTGCCAGCA AAAGACAATT GAGGAGCTGG AGGGCCAGAG GGAAATGCAG 
AGGGCAGCTC TGACCCATCT GACACTGGAC CTGGAGGAAA GGAGCCAGGA GCTGCAGGTA 
CAGGGCAGCC AGATCCGGGA GCTAGAGAGT CACAACAGCC TCTTGGCAAG AGAGCTCCAG 
GAGAAGGACC AGGAGATGAC GTCCCAGCAA GACCAGACAG AGGAACTGCA GAGGCAGAAG 
GAGCATTTGA CACAGGACCT CAAGAGGCGG GAACAGGAGA TAGTGCTGCT GAAGGAGAGG 
ATTCAGGTCT TGGAAGATCA GAGAACCCTA CAGACCAAGA TCCTAGAGGA GGACCTGGAG 
CAGATCAAAA TGTCCTTGAG GGAGCGAGGC CGGGACCTGG CCTCGCAGAG GCAGCTGATG 
CAGGAGCGGG CAGAGGAAGG GAAGGGCCAG AGTAAAGCAC AGCGCGGGAG CCTGGAGCAT 
ATGAAGCTGA TCCTGCGTGA TAAGGAGAAG GAGGTGGAAT GCCAGCAGGA GCATATCCAG 
GAACTGCAGG AGCACAAGGC CCAGCTGGAG CAGCAGCTCC AGGGCTTACA CAGGAAGATG 
GGGGAGACCA GCCTTCTCCT GACCCAGCGG GAGCAGGAGA TAGTGGCTCT GCAGCGGCAC 
CTGCAGGAAG CCAGGGAACA GGGAGAGCTG AAAGAGCAGT CACTTCAAGG TCAGCTGGAA 
GAGGCCCAGA GGGCGCTGGC CCGAAGGGAC CAGGAGCTCA AGGCCCTGCA GCAGCAGCAG 
GCCCAAGGCC AGGAGGAAAA TGTGATGGAA AAGGCGGGCT TACTGCAGAG AGAGCTGGAG 
CAGGCCCACG TGACACTGAA GGAGCGCCAG GCGGAGCTTG AGGATTACAA GGAGCATGTG 
AGAAGGCTCC AGGAAGAGCT GGCAGTGGAG GGGCAGCGGG TACAGGCCCT GGAGGAGGTG 
TTGAGTGACC TAAGGGCTGA ATCTCGGGAA CAAGAGAAGG CCCTGCTGGC CCTCCAGCAG 
CAGTGTGCCG AGCAGGCCCA GGAGCACGAG GCAGAGGCCA GGGCCCTGCA GGACAGCTGG 
CTGCAGGCAG AGGCTATGCT CAAGGAACGG GACCAGGAAC TGGAGGCCCT GCGGGCAGAG 
GGCCTGTCTT CCCAGCATCG GGAGGAGGCT GCCTGTGGCC AGGTGGAGGC TCTGCAGGAG 
GCCCTCAGCA AGGCTCAGGC TGACCTGCAG GAGAAAGAGC AGCATCTCCT TGGGCAGGTG 
GAACTGAGCC GCAGCCTAGA GGCCAGCACG GCCACGTTGC AGGCTGCCCT GGACTCCTGC 
CAGGCACAGT CCCGGCAGCT GGAGGAGGCC CTGAGGAAGC GGGAAGGTGA GGTCCAGGTC 
CGGGACCTCC GATACCAGGA GGCCATGCAG CAGCTCCAGC AGGCACTTAC CCAGAGGGAT 
GAAGAGCTGA GACAACAGAG GAAACAGGGG CAGCTCCTGG AGAAGTCTCT GGCCCAGAGG 
GGCCAAGAGG ATGCAACCCA AGAGAAGCAA GAAGCAAAGC ACGAGAGAGA AGAGGAAGAG 
ATAAGGAGCC TTCGTGAGAG TCTGCAGGAG CTACAGCTGA CTCTAGCACA AAAGGAAGAG 
GAGATCCTGG AGCTGAGGGA GGCCCAGCAG AGGAAGAGTC TGGAGGACTT GTCTCACAAA 
GGCTGCCCAG GGAAGGAGCC GGCTACAACA TTTGATTCCT TAGGGCCCAG GCTGCAGCGG 
GAACTGGAGC GACTACAGGC AGCACTGAAG CAGACAGAAG CCAGGGAGAT CGAGTGGAGG 
GAGAAGGCCC AGGATCTGGC GCTGTCCCTG GCCCAGAGCA AGGCCAGTGT CAGCGATCTG 
CAGGAGGTAG CCCTGTTCCT GCAAGCATCT GTTCTGGAGC GGGACTCAGA ACAGCAGAGG 
CTGCAGGATG AGTTGGAGCT CACCAAACTC ACTCTGGAGA AAGAGCGACT CCACAGCCCA 
GGCCCGAGCA GCAGAGCAGA ACGGGGGCCT AGAGGAGAGC CAGGTGTACA ACTGGGAGAG 
GTCTCGGGAG TGAAGACTGA GCCTAGTCCT GAGATGGAGG AGAGGCAGCT CTGGGAACAG 
AGGCTTGAGC ACCTGCAGCG AGCGGTGGCT CTCTTGGAGG TTGACCGAAG CCAGCTGCAA 
CAACATAACG CCCAGCTGCG GACCACCCTG GAGCAGGTGG AGCGAGAGCG GAGGAAACTG 
AAGAGGGGTT CCATGCGTGC ATTGCGGGCA GGGGACCTGG AGAGCGGTGA AGCCACGGCA 
CCATCACCCA TGCAACAGGA TGGAAGAGGA AGGCAGAAGG GCTGCTCAGA TGCCAAGCAC 
ATGGTTGAGC TGCAAAAAGA GGTGGCCCTC CTGCGAGCCC AGCTGGCCTT GGAGCGGAAG 
CAGAAGCAGG ACTACATTGC TCGCTCAGTT CAGACGAGCC GTGAGCTGGC GGACCTGCAC 
CACGGCCTCT CTCACTCCCT TCTCGCTGTA GCCCAAGCCC CCGAGGCCAC CGTCTTAGAG 
GCCGAGACCC GCAAGCTGGA CGAGTCTCTG ACTCAAAGCC TGACATCCCC CGGGCCAGCA 
CTGCTGTGCC CCAGCTCCAG TGCTGCTCAG ACTGCCTCCA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_021078260.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_020933919.1 Sus scrofa centrosomal protein 250 (CEP250), transcript variant X9, mRNA.

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CloneID	OSe44861
Clone ID Related Accession (Same CDS sequence)	XM_021078260.1 , XM_013985314.1
Accession Version	XM_021078260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-12
Organism	Sus scrofa(pig)
Product	centrosome-associated protein CEP250 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_010459.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCA AGGCTGAGCA CGTGAAGCTC TCTGGGTCCC TGTTGACCTG TTGTCTGCGC 
TTGACTGTGG GAGCACCACA GGCCCGGGAA TCCACTGGAT CTGGGAGGCT GAATGGGAGT 
GAACCATCCC AGCTGATGCT GCTACTGTCT AAGACCCAGG AGCTGGAGAA GGAAGCCCAT 
AAAAGGAGCC AGGAGTTAAT ACAGCTTAAG AGTCAAGGGG ATCTGGAGAA GGCTGAGCTT 
CAGGATCGGG TGACCGAGCT TTCTGCTCTG CTGACCCAGT CTCAGAAGCA GAATGAAGAT 
TATGAAAAGA TGGTAAAGGC TCTGAGAGAG ACAATGAAGA TCCTGGAGGC AAATCATGCA 
GAGTTAATGG AACATGAGGC ATCTCTTAGT AGGAATGCCC AAGAGGAGAG GCTGTCTTTA 
CAGCAAGTGA TCAAGGATAT AACCCAGGCC ATGGTAGAAG AAGGGGACCA TATGGCCCAA 
GGCTCTGGTC ACGAGCACTC CTTGGACTTG GATCCTAGTG GCTTCTTCTC CCAGTTTGAT 
TCCCAGCAGC CAGACAAGGC CCTCACTCTG GTGCGTTCTG TGTTGACTCA GAGACGCCAG 
GCTGTGCAGG ACCTAAAGCA GCAGCTTTCA GGCTGTCAGG AAGCTGTGAG CTCCTTGCAG 
CAGCAGCATC ATCAGTGGGA AGAAGAGGGT GAGGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACCGGGGAGC GGGACACCCT GGCAGGGCAG ACTGTGGGCC TGCAGGGGGA GGTGGACTCT 
CTGAGCAGGG AGCGAGAGCT CCTGCAAAAG ACCAGGGAGG AGCTGCAGCA GCAGCTGGAG 
GTGCTGGAGC AGGAGGCCTG GCGACTACGG AGGACAAACA TGGAGCTTCA GCTGCAGGGC 
GACTCTGCTC AGGGCGAGAA GGAGGAGCAG CAGGAGGAGC TGCACCTGGC TGTCCGAGAA 
CGGGAGCGCC TTCAGGAGAT GCTGGCGAGC CTGGAAGCCA AACAGTCAGA ATCTCTCAGT 
GAACTGATCA CCCTTCGGGA AGCCCTGGAG TCTAGTCGCC TAGAAGGGGA GTTGCTGAGG 
CAAGAGCAAA CAGAAGTGAC TGCTGTGCTA GCAAAGGCAG AACAGTCTAT TGTGGAGCTG 
TCAAGTTCTG AGAACAGCCT GAAGGCTGAG GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCCTTA GATAAAGTTG GACTGAACCA GCAGCTTCTC 
CAGTTAGAAC AGCAGAACCA GTCTGTGTGC AGCAGGATGG AGGCAGCAGA GCAGGCAAGA 
AGCGCTTTGC AGGTGGGCCT GGCAGAGGTG GAGAGGAGCA GGGAAGCGCT GTGGGAAAAG 
AACACTTACC TGGAGGCTCA GCTGCAAAAA GCAGAGGCGG CCAGGGCTGA GCTGCAGGCA 
GATCTCAGGG GCGTCCAGGA AGAGAAGGAA GAAATTCAAG AGAAACTAAG CGAGGCACAT 
CACCAGCAGG AGGCAGTCTC AGTTCAGATG GAGCAGCTGC GTCAGGAGGC AAAGCGGCAG 
GAAGAGGTGC TCGCCCGGGA AGTCCAGGAG AAGGAGGCTC TGGTGCGGGA AAAGGCGGCT 
CTTGAAGTGC GGCTGCAGGC TGTGGAGCGG GACCGGCAGG ACCTCTCTGA ACAACTGCTG 
GGGCTCAGCT CGGCCAAGGA GCTTCTGGAG AACAGTCTGT TTGAGGCCCA GCAACAGAAT 
TCTCTGATAG AAGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTAT CACTCAAGCC 
AAGGAGGTAA TCCAAGGGGA AGTGAGGTGC CTACAGCTGG AACTGGATAC TGAACGGAGC 
CGGGCAGAAC AGGAGCGGGA TGCTGCAACC AAACAGCTGG CCCAGGCTGA GCAAGAGGGG 
CAGACCGCCC TGCAGCAGCA GAAGTCAGCC CATGAGGAGG AAGTGAAGGG GCTCCAGGAA 
AAATGGGAGA AAGAGCGTGC CTGGCACCAG CAGGAACTGG ATAAGGCTCT GAAGAGCCTT 
GAGAGGGAGA AAACGGAGCT GGAAATGAGG CTGAGGGAGC AGCAGGCAGA AACAGAGGCC 
ATGCGGACGC AGAGGGAAGA AGAACGGGCT GAGGCTGAGA GCACCCTGCG CCAGATGCAG 
CTCGAAACAG AGAAGGAGAG AATGTCCCTC CTGGAGACAC TGCTGCAGAC CCAGAAGGAG 
CTGGCAGATG CCAGCCAACA ACTGGAGCGG CTGAGGCAGG ACATGAAGGT TCAGAAGTTA 
AAGGAGCAGG AGACCACTGG GATGCTGCAG ACCCAGCTCC GGGAAGCTCA GAAGGAGCTG 
GAACAGGCAG CCCAGCAGCA CAGAGACAGC CTTGCTGCCC TCCAAGAAGA AGGCCGTGCC 
CTGCTGCAGG ATAAGATAGA CCTGCACAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTT 
TCCAAGGAAG ACTCCCAAAG GCTGGTAGAC CAGGAAGTTC AGGAGAAGCT GAGAGAGGCC 
CAGGAATGTA GCCGAATTCA GAAGGAGCTG GAGAAAGAAA AGGCCAGCCT GACTCTGTTG 
CTGGTGGAAA AGGAACAGAG ACTCCTTGGT TTACAAGAGG CCGACTCCAT TCGACAACAA 
GAGCTGAGCT CCCTGCGCCA GGACATGCAG GAGGCCCAGG CAGGGCAGAA AGAGCTCAGC 
GCCCAGGTGG AATTACTGAA GCAGGAGGTG AAGGCAAAGG AGACTGACTT TCTGGCCCAG 
GAAGCGCGGC TGCTGGAAGA GCTGGAGGCA TCTCAAGTAA CAGAGCAGCA GCTACGAGCT 
TCCTTGTGGG CCCAGGAAGC CAAGGCCGCA CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTGG AGGTGCTGCT GGCAGAGCAG CAGCCTGGGC ACCAGGCCCA GGCCCAGCTG 
GCCAGCCTTT GTGCTGTCCT GCAGCAGGCC CTGGGGTCTG TTTGTGAGAG CAGGCCTGAG 
CTGAGTGGCG GGGGAGACTC TGCTCCCTCC CTCTGGGGCC CTGAGCCAGA CCAGAATGGA 
GCTAGGACCC TTTTCAAGAG AGGGCCCCTC CTGACAGCTC TCTCGGCTGA GGCGGTGGCC 
TCTGCCCTTC ACAAGCTTAA CCAAGACCTG TGGAAGACTC AGCAGGCCCG GGATGATCTG 
AGGGATCAGG CCCAGAAGCT GGGACAACGT CTTACTGAAA CAGAGGCCGA GAAGAGCCAG 
CTCCACACAG AGTTGCAGGC TCTGCAGAGG CAGCTCTCCC AGAACCAGGA AGAGAGATCC 
AAGTGGGAAG GAAAACAGAC CTCCTTAGAA TCTGAGCTGG TGGAACTGCA TGAAACTGTG 
ACATCCTTAC AGAGTCGCCT ACGGCAAGCA GAGCTGCAGG GAATAGAAGC CCAGAATGAG 
CAAGAGTTAC TGCAGGCAGC CAAGGAGAAC CTGACAGCCC AGGTGGAACA CCTCCAAGCA 
GCTGTTGCAG AAGCCAGGGC TCAGGCAAGC GCTGCCGAGG TCCTGGAGGA AGACCTCAAA 
ACTGCACGCT CAGCCCTGAA ACTGAAAAGC AAGGAGGTGG AGAGTGAGCG TGAGCGAGCC 
CAGGCCCTAC AGGAGCAGGG CGAGCTGAAG GTGGCCCAAG GGAAAGCTTT GCAGGAGAAT 
TTGGCTCGCC TGACTCAGAC CCTATCTGAG AGAGAAGGGG AGGTGGAAAC TTTGCAGGGA 
AAAATCCAGG AGCTGGAGAA GCAACGGGAA ATGCAAAAGG CTGCTTTGGA GGTGCTGTCT 
TTGGACCTCA AAAAGAGGAA CCAAGAGGTG GATCTGCAGC AAGAACAGAT TCAGGAGCTG 
GAGGAGTGCA GATCGGTTTT AGAGCATCTC CCCATGGCAG TCCAGGAGCG AGAGCAGAAG 
CTGACTCTGC AGAGGGAGCA GATCAAGGAG CTCGAGAAGG ACCGAGAGAC ACAGAGGAAC 
ATCTTGGAGC ATCAGCTTCT GGAACTTGAG AAGAAGGCCC AAGTGATTGA GTCCCAGAAA 
GGACAGATTC AGGATCTGAA GAAGCAGCTG GTTACTCTGG AATGCCTGGC CCTGGAACTA 
GAGGAAAACC ATCACAAAAT AGAGTGCCAG CAAAAGACAA TTGAGGAGCT GGAGGGCCAG 
AGGGAAATGC AGAGGGCAGC TCTGACCCAT CTGACACTGG ACCTGGAGGA AAGGAGCCAG 
GAGCTGCAGG TACAGGGCAG CCAGATCCGG GAGCTAGAGA GTCACAACAG CCTCTTGGCA 
AGAGAGCTCC AGGAGAAGGA CCAGGAGATG ACGTCCCAGC AAGACCAGAC AGAGGAACTG 
CAGAGGCAGA AGGAGCATTT GACACAGGAC CTCAAGAGGC GGGAACAGGA GATAGTGCTG 
CTGAAGGAGA GGATTCAGGT CTTGGAAGAT CAGAGAACCC TACAGACCAA GATCCTAGAG 
GAGGACCTGG AGCAGATCAA AATGTCCTTG AGGGAGCGAG GCCGGGACCT GGCCTCGCAG 
AGGCAGCTGA TGCAGGAGCG GGCAGAGGAA GGGAAGGGCC AGAGTAAAGC ACAGCGCGGG 
AGCCTGGAGC ATATGAAGCT GATCCTGCGT GATAAGGAGA AGGAGGTGGA ATGCCAGCAG 
GAGCATATCC AGGAACTGCA GGAGCACAAG GCCCAGCTGG AGCAGCAGCT CCAGGGCTTA 
CACAGGAAGA TGGGGGAGAC CAGCCTTCTC CTGACCCAGC GGGAGCAGGA GATAGTGGCT 
CTGCAGCGGC ACCTGCAGGA AGCCAGGGAA CAGGGAGAGC TGAAAGAGCA GTCACTTCAA 
GGTCAGCTGG AAGAGGCCCA GAGGGCGCTG GCCCGAAGGG ACCAGGAGCT CAAGGCCCTG 
CAGCAGCAGC AGGCCCAAGG CCAGGAGGAA AATGTGATGG AAAAGGCGGG CTTACTGCAG 
AGAGAGCTGG AGCAGGCCCA CGTGACACTG AAGGAGCGCC AGGCGGAGCT TGAGGATTAC 
AAGGAGCATG TGAGAAGGCT CCAGGAAGAG CTGGCAGTGG AGGGGCAGCG GGTACAGGCC 
CTGGAGGAGG TGTTGAGTGA CCTAAGGGCT GAATCTCGGG AACAAGAGAA GGCCCTGCTG 
GCCCTCCAGC AGCAGTGTGC CGAGCAGGCC CAGGAGCACG AGGCAGAGGC CAGGGCCCTG 
CAGGACAGCT GGCTGCAGGC AGAGGCTATG CTCAAGGAAC GGGACCAGGA ACTGGAGGCC 
CTGCGGGCAG AGGGCCTGTC TTCCCAGCAT CGGGAGGAGG CTGCCTGTGG CCAGGTGGAG 
GCTCTGCAGG AGGCCCTCAG CAAGGCTCAG GCTGACCTGC AGGAGAAAGA GCAGCATCTC 
CTTGGGCAGG TGGAACTGAG CCGCAGCCTA GAGGCCAGCA CGGCCACGTT GCAGGCTGCC 
CTGGACTCCT GCCAGGCACA GTCCCGGCAG CTGGAGGAGG CCCTGAGGAA GCGGGAAGGT 
GAGGTCCAGG TCCGGGACCT CCGATACCAG GAGGCCATGC AGCAGCTCCA GCAGGCACTT 
ACCCAGAGGG ATGAAGAGCT GAGACAACAG AGGAAACAGG GGCAGCTCCT GGAGAAGTCT 
CTGGCCCAGA GGGGCCAAGA GGATGCAACC CAAGAGAAGC AAGAAGCAAA GCACGAGAGA 
GAAGAGGAAG AGATAAGGAG CCTTCGTGAG AGTCTGCAGG AGCTACAGCT GACTCTAGCA 
CAAAAGGAAG AGGAGATCCT GGAGCTGAGG GAGGCCCAGC AGAGGAAGAG TCTGGAGGAC 
TTGTCTCACA AAGGCTGCCC AGGGAAGGAG CCGGCTACAA CATTTGATTC CTTAGGGCCC 
AGGCTGCAGC GGGAACTGGA GCGACTACAG GCAGCACTGA AGCAGACAGA AGCCAGGGAG 
ATCGAGTGGA GGGAGAAGGC CCAGGATCTG GCGCTGTCCC TGGCCCAGAG CAAGGCCAGT 
GTCAGCGATC TGCAGGAGGT AGCCCTGTTC CTGCAAGCAT CTGTTCTGGA GCGGGACTCA 
GAACAGCAGA GGCTGCAGGA TGAGTTGGAG CTCACCAAAC TCACTCTGGA GAAAGAGCGA 
CTCCACAGCC CAGGCCCGAG CAGCAGAGCA GAACGGGGGC CTAGAGGAGA GCCAGGTGTA 
CAACTGGGAG AGGTCTCGGG AGTGAAGACT GAGCCTAGTC CTGAGATGGA GGAGAGGCAG 
CTCTGGGAAC AGAGGCTTGA GCACCTGCAG CGAGCGGTGG CTCTCTTGGA GGTTGACCGA 
AGCCAGCTGC AACAACATAA CGCCCAGCTG CGGACCACCC TGGAGCAGGT GGAGCGAGAG 
CGGAGGAAAC TGAAGAGGGG TTCCATGCGT GCATTGCGGG CAGGGGACCT GGAGAGCGGT 
GAAGCCACGG CACCATCACC CATGCAACAG GATGGAAGAG GAAGGCAGAA GGGCTGCTCA 
GATGCCAAGC ACATGGTTGA GCTGCAAAAA GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC 
TTGGAGCGGA AGCAGAAGCA GGACTACATT GCTCGCTCAG TTCAGACGAG CCGTGAGCTG 
GCGGACCTGC ACCACGGCCT CTCTCACTCC CTTCTCGCTG TAGCCCAAGC CCCCGAGGCC 
ACCGTCTTAG AGGCCGAGAC CCGCAAGCTG GACGAGTCTC TGACTCAAAG CCTGACATCC 
CCCGGGCCAG CACTGCTGTG CCCCAGCTCC AGTGCTGCTC AGACTGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020933919.1
CDS	673..7389
Translation	MELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_021078260.1
Organism	Sus scrofa(pig)
Definition	Sus scrofa centrosomal protein 250 (CEP250), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021078260.1

ORF Insert Sequence:

ATGGAGCTCA AGGCTGAGCA CGTGAAGCTC TCTGGGTCCC TGTTGACCTG TTGTCTGCGC 
TTGACTGTGG GAGCACCACA GGCCCGGGAA TCCACTGGAT CTGGGAGGCT GAATGGGAGT 
GAACCATCCC AGCTGATGCT GCTACTGTCT AAGACCCAGG AGCTGGAGAA GGAAGCCCAT 
AAAAGGAGCC AGGAGTTAAT ACAGCTTAAG AGTCAAGGGG ATCTGGAGAA GGCTGAGCTT 
CAGGATCGGG TGACCGAGCT TTCTGCTCTG CTGACCCAGT CTCAGAAGCA GAATGAAGAT 
TATGAAAAGA TGGTAAAGGC TCTGAGAGAG ACAATGAAGA TCCTGGAGGC AAATCATGCA 
GAGTTAATGG AACATGAGGC ATCTCTTAGT AGGAATGCCC AAGAGGAGAG GCTGTCTTTA 
CAGCAAGTGA TCAAGGATAT AACCCAGGCC ATGGTAGAAG AAGGGGACCA TATGGCCCAA 
GGCTCTGGTC ACGAGCACTC CTTGGACTTG GATCCTAGTG GCTTCTTCTC CCAGTTTGAT 
TCCCAGCAGC CAGACAAGGC CCTCACTCTG GTGCGTTCTG TGTTGACTCA GAGACGCCAG 
GCTGTGCAGG ACCTAAAGCA GCAGCTTTCA GGCTGTCAGG AAGCTGTGAG CTCCTTGCAG 
CAGCAGCATC ATCAGTGGGA AGAAGAGGGT GAGGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACCGGGGAGC GGGACACCCT GGCAGGGCAG ACTGTGGGCC TGCAGGGGGA GGTGGACTCT 
CTGAGCAGGG AGCGAGAGCT CCTGCAAAAG ACCAGGGAGG AGCTGCAGCA GCAGCTGGAG 
GTGCTGGAGC AGGAGGCCTG GCGACTACGG AGGACAAACA TGGAGCTTCA GCTGCAGGGC 
GACTCTGCTC AGGGCGAGAA GGAGGAGCAG CAGGAGGAGC TGCACCTGGC TGTCCGAGAA 
CGGGAGCGCC TTCAGGAGAT GCTGGCGAGC CTGGAAGCCA AACAGTCAGA ATCTCTCAGT 
GAACTGATCA CCCTTCGGGA AGCCCTGGAG TCTAGTCGCC TAGAAGGGGA GTTGCTGAGG 
CAAGAGCAAA CAGAAGTGAC TGCTGTGCTA GCAAAGGCAG AACAGTCTAT TGTGGAGCTG 
TCAAGTTCTG AGAACAGCCT GAAGGCTGAG GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCCTTA GATAAAGTTG GACTGAACCA GCAGCTTCTC 
CAGTTAGAAC AGCAGAACCA GTCTGTGTGC AGCAGGATGG AGGCAGCAGA GCAGGCAAGA 
AGCGCTTTGC AGGTGGGCCT GGCAGAGGTG GAGAGGAGCA GGGAAGCGCT GTGGGAAAAG 
AACACTTACC TGGAGGCTCA GCTGCAAAAA GCAGAGGCGG CCAGGGCTGA GCTGCAGGCA 
GATCTCAGGG GCGTCCAGGA AGAGAAGGAA GAAATTCAAG AGAAACTAAG CGAGGCACAT 
CACCAGCAGG AGGCAGTCTC AGTTCAGATG GAGCAGCTGC GTCAGGAGGC AAAGCGGCAG 
GAAGAGGTGC TCGCCCGGGA AGTCCAGGAG AAGGAGGCTC TGGTGCGGGA AAAGGCGGCT 
CTTGAAGTGC GGCTGCAGGC TGTGGAGCGG GACCGGCAGG ACCTCTCTGA ACAACTGCTG 
GGGCTCAGCT CGGCCAAGGA GCTTCTGGAG AACAGTCTGT TTGAGGCCCA GCAACAGAAT 
TCTCTGATAG AAGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTAT CACTCAAGCC 
AAGGAGGTAA TCCAAGGGGA AGTGAGGTGC CTACAGCTGG AACTGGATAC TGAACGGAGC 
CGGGCAGAAC AGGAGCGGGA TGCTGCAACC AAACAGCTGG CCCAGGCTGA GCAAGAGGGG 
CAGACCGCCC TGCAGCAGCA GAAGTCAGCC CATGAGGAGG AAGTGAAGGG GCTCCAGGAA 
AAATGGGAGA AAGAGCGTGC CTGGCACCAG CAGGAACTGG ATAAGGCTCT GAAGAGCCTT 
GAGAGGGAGA AAACGGAGCT GGAAATGAGG CTGAGGGAGC AGCAGGCAGA AACAGAGGCC 
ATGCGGACGC AGAGGGAAGA AGAACGGGCT GAGGCTGAGA GCACCCTGCG CCAGATGCAG 
CTCGAAACAG AGAAGGAGAG AATGTCCCTC CTGGAGACAC TGCTGCAGAC CCAGAAGGAG 
CTGGCAGATG CCAGCCAACA ACTGGAGCGG CTGAGGCAGG ACATGAAGGT TCAGAAGTTA 
AAGGAGCAGG AGACCACTGG GATGCTGCAG ACCCAGCTCC GGGAAGCTCA GAAGGAGCTG 
GAACAGGCAG CCCAGCAGCA CAGAGACAGC CTTGCTGCCC TCCAAGAAGA AGGCCGTGCC 
CTGCTGCAGG ATAAGATAGA CCTGCACAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTT 
TCCAAGGAAG ACTCCCAAAG GCTGGTAGAC CAGGAAGTTC AGGAGAAGCT GAGAGAGGCC 
CAGGAATGTA GCCGAATTCA GAAGGAGCTG GAGAAAGAAA AGGCCAGCCT GACTCTGTTG 
CTGGTGGAAA AGGAACAGAG ACTCCTTGGT TTACAAGAGG CCGACTCCAT TCGACAACAA 
GAGCTGAGCT CCCTGCGCCA GGACATGCAG GAGGCCCAGG CAGGGCAGAA AGAGCTCAGC 
GCCCAGGTGG AATTACTGAA GCAGGAGGTG AAGGCAAAGG AGACTGACTT TCTGGCCCAG 
GAAGCGCGGC TGCTGGAAGA GCTGGAGGCA TCTCAAGTAA CAGAGCAGCA GCTACGAGCT 
TCCTTGTGGG CCCAGGAAGC CAAGGCCGCA CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTGG AGGTGCTGCT GGCAGAGCAG CAGCCTGGGC ACCAGGCCCA GGCCCAGCTG 
GCCAGCCTTT GTGCTGTCCT GCAGCAGGCC CTGGGGTCTG TTTGTGAGAG CAGGCCTGAG 
CTGAGTGGCG GGGGAGACTC TGCTCCCTCC CTCTGGGGCC CTGAGCCAGA CCAGAATGGA 
GCTAGGACCC TTTTCAAGAG AGGGCCCCTC CTGACAGCTC TCTCGGCTGA GGCGGTGGCC 
TCTGCCCTTC ACAAGCTTAA CCAAGACCTG TGGAAGACTC AGCAGGCCCG GGATGATCTG 
AGGGATCAGG CCCAGAAGCT GGGACAACGT CTTACTGAAA CAGAGGCCGA GAAGAGCCAG 
CTCCACACAG AGTTGCAGGC TCTGCAGAGG CAGCTCTCCC AGAACCAGGA AGAGAGATCC 
AAGTGGGAAG GAAAACAGAC CTCCTTAGAA TCTGAGCTGG TGGAACTGCA TGAAACTGTG 
ACATCCTTAC AGAGTCGCCT ACGGCAAGCA GAGCTGCAGG GAATAGAAGC CCAGAATGAG 
CAAGAGTTAC TGCAGGCAGC CAAGGAGAAC CTGACAGCCC AGGTGGAACA CCTCCAAGCA 
GCTGTTGCAG AAGCCAGGGC TCAGGCAAGC GCTGCCGAGG TCCTGGAGGA AGACCTCAAA 
ACTGCACGCT CAGCCCTGAA ACTGAAAAGC AAGGAGGTGG AGAGTGAGCG TGAGCGAGCC 
CAGGCCCTAC AGGAGCAGGG CGAGCTGAAG GTGGCCCAAG GGAAAGCTTT GCAGGAGAAT 
TTGGCTCGCC TGACTCAGAC CCTATCTGAG AGAGAAGGGG AGGTGGAAAC TTTGCAGGGA 
AAAATCCAGG AGCTGGAGAA GCAACGGGAA ATGCAAAAGG CTGCTTTGGA GGTGCTGTCT 
TTGGACCTCA AAAAGAGGAA CCAAGAGGTG GATCTGCAGC AAGAACAGAT TCAGGAGCTG 
GAGGAGTGCA GATCGGTTTT AGAGCATCTC CCCATGGCAG TCCAGGAGCG AGAGCAGAAG 
CTGACTCTGC AGAGGGAGCA GATCAAGGAG CTCGAGAAGG ACCGAGAGAC ACAGAGGAAC 
ATCTTGGAGC ATCAGCTTCT GGAACTTGAG AAGAAGGCCC AAGTGATTGA GTCCCAGAAA 
GGACAGATTC AGGATCTGAA GAAGCAGCTG GTTACTCTGG AATGCCTGGC CCTGGAACTA 
GAGGAAAACC ATCACAAAAT AGAGTGCCAG CAAAAGACAA TTGAGGAGCT GGAGGGCCAG 
AGGGAAATGC AGAGGGCAGC TCTGACCCAT CTGACACTGG ACCTGGAGGA AAGGAGCCAG 
GAGCTGCAGG TACAGGGCAG CCAGATCCGG GAGCTAGAGA GTCACAACAG CCTCTTGGCA 
AGAGAGCTCC AGGAGAAGGA CCAGGAGATG ACGTCCCAGC AAGACCAGAC AGAGGAACTG 
CAGAGGCAGA AGGAGCATTT GACACAGGAC CTCAAGAGGC GGGAACAGGA GATAGTGCTG 
CTGAAGGAGA GGATTCAGGT CTTGGAAGAT CAGAGAACCC TACAGACCAA GATCCTAGAG 
GAGGACCTGG AGCAGATCAA AATGTCCTTG AGGGAGCGAG GCCGGGACCT GGCCTCGCAG 
AGGCAGCTGA TGCAGGAGCG GGCAGAGGAA GGGAAGGGCC AGAGTAAAGC ACAGCGCGGG 
AGCCTGGAGC ATATGAAGCT GATCCTGCGT GATAAGGAGA AGGAGGTGGA ATGCCAGCAG 
GAGCATATCC AGGAACTGCA GGAGCACAAG GCCCAGCTGG AGCAGCAGCT CCAGGGCTTA 
CACAGGAAGA TGGGGGAGAC CAGCCTTCTC CTGACCCAGC GGGAGCAGGA GATAGTGGCT 
CTGCAGCGGC ACCTGCAGGA AGCCAGGGAA CAGGGAGAGC TGAAAGAGCA GTCACTTCAA 
GGTCAGCTGG AAGAGGCCCA GAGGGCGCTG GCCCGAAGGG ACCAGGAGCT CAAGGCCCTG 
CAGCAGCAGC AGGCCCAAGG CCAGGAGGAA AATGTGATGG AAAAGGCGGG CTTACTGCAG 
AGAGAGCTGG AGCAGGCCCA CGTGACACTG AAGGAGCGCC AGGCGGAGCT TGAGGATTAC 
AAGGAGCATG TGAGAAGGCT CCAGGAAGAG CTGGCAGTGG AGGGGCAGCG GGTACAGGCC 
CTGGAGGAGG TGTTGAGTGA CCTAAGGGCT GAATCTCGGG AACAAGAGAA GGCCCTGCTG 
GCCCTCCAGC AGCAGTGTGC CGAGCAGGCC CAGGAGCACG AGGCAGAGGC CAGGGCCCTG 
CAGGACAGCT GGCTGCAGGC AGAGGCTATG CTCAAGGAAC GGGACCAGGA ACTGGAGGCC 
CTGCGGGCAG AGGGCCTGTC TTCCCAGCAT CGGGAGGAGG CTGCCTGTGG CCAGGTGGAG 
GCTCTGCAGG AGGCCCTCAG CAAGGCTCAG GCTGACCTGC AGGAGAAAGA GCAGCATCTC 
CTTGGGCAGG TGGAACTGAG CCGCAGCCTA GAGGCCAGCA CGGCCACGTT GCAGGCTGCC 
CTGGACTCCT GCCAGGCACA GTCCCGGCAG CTGGAGGAGG CCCTGAGGAA GCGGGAAGGT 
GAGGTCCAGG TCCGGGACCT CCGATACCAG GAGGCCATGC AGCAGCTCCA GCAGGCACTT 
ACCCAGAGGG ATGAAGAGCT GAGACAACAG AGGAAACAGG GGCAGCTCCT GGAGAAGTCT 
CTGGCCCAGA GGGGCCAAGA GGATGCAACC CAAGAGAAGC AAGAAGCAAA GCACGAGAGA 
GAAGAGGAAG AGATAAGGAG CCTTCGTGAG AGTCTGCAGG AGCTACAGCT GACTCTAGCA 
CAAAAGGAAG AGGAGATCCT GGAGCTGAGG GAGGCCCAGC AGAGGAAGAG TCTGGAGGAC 
TTGTCTCACA AAGGCTGCCC AGGGAAGGAG CCGGCTACAA CATTTGATTC CTTAGGGCCC 
AGGCTGCAGC GGGAACTGGA GCGACTACAG GCAGCACTGA AGCAGACAGA AGCCAGGGAG 
ATCGAGTGGA GGGAGAAGGC CCAGGATCTG GCGCTGTCCC TGGCCCAGAG CAAGGCCAGT 
GTCAGCGATC TGCAGGAGGT AGCCCTGTTC CTGCAAGCAT CTGTTCTGGA GCGGGACTCA 
GAACAGCAGA GGCTGCAGGA TGAGTTGGAG CTCACCAAAC TCACTCTGGA GAAAGAGCGA 
CTCCACAGCC CAGGCCCGAG CAGCAGAGCA GAACGGGGGC CTAGAGGAGA GCCAGGTGTA 
CAACTGGGAG AGGTCTCGGG AGTGAAGACT GAGCCTAGTC CTGAGATGGA GGAGAGGCAG 
CTCTGGGAAC AGAGGCTTGA GCACCTGCAG CGAGCGGTGG CTCTCTTGGA GGTTGACCGA 
AGCCAGCTGC AACAACATAA CGCCCAGCTG CGGACCACCC TGGAGCAGGT GGAGCGAGAG 
CGGAGGAAAC TGAAGAGGGG TTCCATGCGT GCATTGCGGG CAGGGGACCT GGAGAGCGGT 
GAAGCCACGG CACCATCACC CATGCAACAG GATGGAAGAG GAAGGCAGAA GGGCTGCTCA 
GATGCCAAGC ACATGGTTGA GCTGCAAAAA GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC 
TTGGAGCGGA AGCAGAAGCA GGACTACATT GCTCGCTCAG TTCAGACGAG CCGTGAGCTG 
GCGGACCTGC ACCACGGCCT CTCTCACTCC CTTCTCGCTG TAGCCCAAGC CCCCGAGGCC 
ACCGTCTTAG AGGCCGAGAC CCGCAAGCTG GACGAGTCTC TGACTCAAAG CCTGACATCC 
CCCGGGCCAG CACTGCTGTG CCCCAGCTCC AGTGCTGCTC AGACTGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP250 ( XM_013985314.1 ) cDNA ORF clone, Sus scrofa(pig) -> XP_013840768.1 Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X9, mRNA.

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CloneID	OSe44861
Clone ID Related Accession (Same CDS sequence)	XM_021078260.1 , XM_013985314.1
Accession Version	XM_013985314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-10
Organism	Sus scrofa(Pig)
Product	centrosome-associated protein CEP250 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003612568.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sus scrofa Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTCA AGGCTGAGCA CGTGAAGCTC TCTGGGTCCC TGTTGACCTG TTGTCTGCGC 
TTGACTGTGG GAGCACCACA GGCCCGGGAA TCCACTGGAT CTGGGAGGCT GAATGGGAGT 
GAACCATCCC AGCTGATGCT GCTACTGTCT AAGACCCAGG AGCTGGAGAA GGAAGCCCAT 
AAAAGGAGCC AGGAGTTAAT ACAGCTTAAG AGTCAAGGGG ATCTGGAGAA GGCTGAGCTT 
CAGGATCGGG TGACCGAGCT TTCTGCTCTG CTGACCCAGT CTCAGAAGCA GAATGAAGAT 
TATGAAAAGA TGGTAAAGGC TCTGAGAGAG ACAATGAAGA TCCTGGAGGC AAATCATGCA 
GAGTTAATGG AACATGAGGC ATCTCTTAGT AGGAATGCCC AAGAGGAGAG GCTGTCTTTA 
CAGCAAGTGA TCAAGGATAT AACCCAGGCC ATGGTAGAAG AAGGGGACCA TATGGCCCAA 
GGCTCTGGTC ACGAGCACTC CTTGGACTTG GATCCTAGTG GCTTCTTCTC CCAGTTTGAT 
TCCCAGCAGC CAGACAAGGC CCTCACTCTG GTGCGTTCTG TGTTGACTCA GAGACGCCAG 
GCTGTGCAGG ACCTAAAGCA GCAGCTTTCA GGCTGTCAGG AAGCTGTGAG CTCCTTGCAG 
CAGCAGCATC ATCAGTGGGA AGAAGAGGGT GAGGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACCGGGGAGC GGGACACCCT GGCAGGGCAG ACTGTGGGCC TGCAGGGGGA GGTGGACTCT 
CTGAGCAGGG AGCGAGAGCT CCTGCAAAAG ACCAGGGAGG AGCTGCAGCA GCAGCTGGAG 
GTGCTGGAGC AGGAGGCCTG GCGACTACGG AGGACAAACA TGGAGCTTCA GCTGCAGGGC 
GACTCTGCTC AGGGCGAGAA GGAGGAGCAG CAGGAGGAGC TGCACCTGGC TGTCCGAGAA 
CGGGAGCGCC TTCAGGAGAT GCTGGCGAGC CTGGAAGCCA AACAGTCAGA ATCTCTCAGT 
GAACTGATCA CCCTTCGGGA AGCCCTGGAG TCTAGTCGCC TAGAAGGGGA GTTGCTGAGG 
CAAGAGCAAA CAGAAGTGAC TGCTGTGCTA GCAAAGGCAG AACAGTCTAT TGTGGAGCTG 
TCAAGTTCTG AGAACAGCCT GAAGGCTGAG GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCCTTA GATAAAGTTG GACTGAACCA GCAGCTTCTC 
CAGTTAGAAC AGCAGAACCA GTCTGTGTGC AGCAGGATGG AGGCAGCAGA GCAGGCAAGA 
AGCGCTTTGC AGGTGGGCCT GGCAGAGGTG GAGAGGAGCA GGGAAGCGCT GTGGGAAAAG 
AACACTTACC TGGAGGCTCA GCTGCAAAAA GCAGAGGCGG CCAGGGCTGA GCTGCAGGCA 
GATCTCAGGG GCGTCCAGGA AGAGAAGGAA GAAATTCAAG AGAAACTAAG CGAGGCACAT 
CACCAGCAGG AGGCAGTCTC AGTTCAGATG GAGCAGCTGC GTCAGGAGGC AAAGCGGCAG 
GAAGAGGTGC TCGCCCGGGA AGTCCAGGAG AAGGAGGCTC TGGTGCGGGA AAAGGCGGCT 
CTTGAAGTGC GGCTGCAGGC TGTGGAGCGG GACCGGCAGG ACCTCTCTGA ACAACTGCTG 
GGGCTCAGCT CGGCCAAGGA GCTTCTGGAG AACAGTCTGT TTGAGGCCCA GCAACAGAAT 
TCTCTGATAG AAGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTAT CACTCAAGCC 
AAGGAGGTAA TCCAAGGGGA AGTGAGGTGC CTACAGCTGG AACTGGATAC TGAACGGAGC 
CGGGCAGAAC AGGAGCGGGA TGCTGCAACC AAACAGCTGG CCCAGGCTGA GCAAGAGGGG 
CAGACCGCCC TGCAGCAGCA GAAGTCAGCC CATGAGGAGG AAGTGAAGGG GCTCCAGGAA 
AAATGGGAGA AAGAGCGTGC CTGGCACCAG CAGGAACTGG ATAAGGCTCT GAAGAGCCTT 
GAGAGGGAGA AAACGGAGCT GGAAATGAGG CTGAGGGAGC AGCAGGCAGA AACAGAGGCC 
ATGCGGACGC AGAGGGAAGA AGAACGGGCT GAGGCTGAGA GCACCCTGCG CCAGATGCAG 
CTCGAAACAG AGAAGGAGAG AATGTCCCTC CTGGAGACAC TGCTGCAGAC CCAGAAGGAG 
CTGGCAGATG CCAGCCAACA ACTGGAGCGG CTGAGGCAGG ACATGAAGGT TCAGAAGTTA 
AAGGAGCAGG AGACCACTGG GATGCTGCAG ACCCAGCTCC GGGAAGCTCA GAAGGAGCTG 
GAACAGGCAG CCCAGCAGCA CAGAGACAGC CTTGCTGCCC TCCAAGAAGA AGGCCGTGCC 
CTGCTGCAGG ATAAGATAGA CCTGCACAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTT 
TCCAAGGAAG ACTCCCAAAG GCTGGTAGAC CAGGAAGTTC AGGAGAAGCT GAGAGAGGCC 
CAGGAATGTA GCCGAATTCA GAAGGAGCTG GAGAAAGAAA AGGCCAGCCT GACTCTGTTG 
CTGGTGGAAA AGGAACAGAG ACTCCTTGGT TTACAAGAGG CCGACTCCAT TCGACAACAA 
GAGCTGAGCT CCCTGCGCCA GGACATGCAG GAGGCCCAGG CAGGGCAGAA AGAGCTCAGC 
GCCCAGGTGG AATTACTGAA GCAGGAGGTG AAGGCAAAGG AGACTGACTT TCTGGCCCAG 
GAAGCGCGGC TGCTGGAAGA GCTGGAGGCA TCTCAAGTAA CAGAGCAGCA GCTACGAGCT 
TCCTTGTGGG CCCAGGAAGC CAAGGCCGCA CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTGG AGGTGCTGCT GGCAGAGCAG CAGCCTGGGC ACCAGGCCCA GGCCCAGCTG 
GCCAGCCTTT GTGCTGTCCT GCAGCAGGCC CTGGGGTCTG TTTGTGAGAG CAGGCCTGAG 
CTGAGTGGCG GGGGAGACTC TGCTCCCTCC CTCTGGGGCC CTGAGCCAGA CCAGAATGGA 
GCTAGGACCC TTTTCAAGAG AGGGCCCCTC CTGACAGCTC TCTCGGCTGA GGCGGTGGCC 
TCTGCCCTTC ACAAGCTTAA CCAAGACCTG TGGAAGACTC AGCAGGCCCG GGATGATCTG 
AGGGATCAGG CCCAGAAGCT GGGACAACGT CTTACTGAAA CAGAGGCCGA GAAGAGCCAG 
CTCCACACAG AGTTGCAGGC TCTGCAGAGG CAGCTCTCCC AGAACCAGGA AGAGAGATCC 
AAGTGGGAAG GAAAACAGAC CTCCTTAGAA TCTGAGCTGG TGGAACTGCA TGAAACTGTG 
ACATCCTTAC AGAGTCGCCT ACGGCAAGCA GAGCTGCAGG GAATAGAAGC CCAGAATGAG 
CAAGAGTTAC TGCAGGCAGC CAAGGAGAAC CTGACAGCCC AGGTGGAACA CCTCCAAGCA 
GCTGTTGCAG AAGCCAGGGC TCAGGCAAGC GCTGCCGAGG TCCTGGAGGA AGACCTCAAA 
ACTGCACGCT CAGCCCTGAA ACTGAAAAGC AAGGAGGTGG AGAGTGAGCG TGAGCGAGCC 
CAGGCCCTAC AGGAGCAGGG CGAGCTGAAG GTGGCCCAAG GGAAAGCTTT GCAGGAGAAT 
TTGGCTCGCC TGACTCAGAC CCTATCTGAG AGAGAAGGGG AGGTGGAAAC TTTGCAGGGA 
AAAATCCAGG AGCTGGAGAA GCAACGGGAA ATGCAAAAGG CTGCTTTGGA GGTGCTGTCT 
TTGGACCTCA AAAAGAGGAA CCAAGAGGTG GATCTGCAGC AAGAACAGAT TCAGGAGCTG 
GAGGAGTGCA GATCGGTTTT AGAGCATCTC CCCATGGCAG TCCAGGAGCG AGAGCAGAAG 
CTGACTCTGC AGAGGGAGCA GATCAAGGAG CTCGAGAAGG ACCGAGAGAC ACAGAGGAAC 
ATCTTGGAGC ATCAGCTTCT GGAACTTGAG AAGAAGGCCC AAGTGATTGA GTCCCAGAAA 
GGACAGATTC AGGATCTGAA GAAGCAGCTG GTTACTCTGG AATGCCTGGC CCTGGAACTA 
GAGGAAAACC ATCACAAAAT AGAGTGCCAG CAAAAGACAA TTGAGGAGCT GGAGGGCCAG 
AGGGAAATGC AGAGGGCAGC TCTGACCCAT CTGACACTGG ACCTGGAGGA AAGGAGCCAG 
GAGCTGCAGG TACAGGGCAG CCAGATCCGG GAGCTAGAGA GTCACAACAG CCTCTTGGCA 
AGAGAGCTCC AGGAGAAGGA CCAGGAGATG ACGTCCCAGC AAGACCAGAC AGAGGAACTG 
CAGAGGCAGA AGGAGCATTT GACACAGGAC CTCAAGAGGC GGGAACAGGA GATAGTGCTG 
CTGAAGGAGA GGATTCAGGT CTTGGAAGAT CAGAGAACCC TACAGACCAA GATCCTAGAG 
GAGGACCTGG AGCAGATCAA AATGTCCTTG AGGGAGCGAG GCCGGGACCT GGCCTCGCAG 
AGGCAGCTGA TGCAGGAGCG GGCAGAGGAA GGGAAGGGCC AGAGTAAAGC ACAGCGCGGG 
AGCCTGGAGC ATATGAAGCT GATCCTGCGT GATAAGGAGA AGGAGGTGGA ATGCCAGCAG 
GAGCATATCC AGGAACTGCA GGAGCACAAG GCCCAGCTGG AGCAGCAGCT CCAGGGCTTA 
CACAGGAAGA TGGGGGAGAC CAGCCTTCTC CTGACCCAGC GGGAGCAGGA GATAGTGGCT 
CTGCAGCGGC ACCTGCAGGA AGCCAGGGAA CAGGGAGAGC TGAAAGAGCA GTCACTTCAA 
GGTCAGCTGG AAGAGGCCCA GAGGGCGCTG GCCCGAAGGG ACCAGGAGCT CAAGGCCCTG 
CAGCAGCAGC AGGCCCAAGG CCAGGAGGAA AATGTGATGG AAAAGGCGGG CTTACTGCAG 
AGAGAGCTGG AGCAGGCCCA CGTGACACTG AAGGAGCGCC AGGCGGAGCT TGAGGATTAC 
AAGGAGCATG TGAGAAGGCT CCAGGAAGAG CTGGCAGTGG AGGGGCAGCG GGTACAGGCC 
CTGGAGGAGG TGTTGAGTGA CCTAAGGGCT GAATCTCGGG AACAAGAGAA GGCCCTGCTG 
GCCCTCCAGC AGCAGTGTGC CGAGCAGGCC CAGGAGCACG AGGCAGAGGC CAGGGCCCTG 
CAGGACAGCT GGCTGCAGGC AGAGGCTATG CTCAAGGAAC GGGACCAGGA ACTGGAGGCC 
CTGCGGGCAG AGGGCCTGTC TTCCCAGCAT CGGGAGGAGG CTGCCTGTGG CCAGGTGGAG 
GCTCTGCAGG AGGCCCTCAG CAAGGCTCAG GCTGACCTGC AGGAGAAAGA GCAGCATCTC 
CTTGGGCAGG TGGAACTGAG CCGCAGCCTA GAGGCCAGCA CGGCCACGTT GCAGGCTGCC 
CTGGACTCCT GCCAGGCACA GTCCCGGCAG CTGGAGGAGG CCCTGAGGAA GCGGGAAGGT 
GAGGTCCAGG TCCGGGACCT CCGATACCAG GAGGCCATGC AGCAGCTCCA GCAGGCACTT 
ACCCAGAGGG ATGAAGAGCT GAGACAACAG AGGAAACAGG GGCAGCTCCT GGAGAAGTCT 
CTGGCCCAGA GGGGCCAAGA GGATGCAACC CAAGAGAAGC AAGAAGCAAA GCACGAGAGA 
GAAGAGGAAG AGATAAGGAG CCTTCGTGAG AGTCTGCAGG AGCTACAGCT GACTCTAGCA 
CAAAAGGAAG AGGAGATCCT GGAGCTGAGG GAGGCCCAGC AGAGGAAGAG TCTGGAGGAC 
TTGTCTCACA AAGGCTGCCC AGGGAAGGAG CCGGCTACAA CATTTGATTC CTTAGGGCCC 
AGGCTGCAGC GGGAACTGGA GCGACTACAG GCAGCACTGA AGCAGACAGA AGCCAGGGAG 
ATCGAGTGGA GGGAGAAGGC CCAGGATCTG GCGCTGTCCC TGGCCCAGAG CAAGGCCAGT 
GTCAGCGATC TGCAGGAGGT AGCCCTGTTC CTGCAAGCAT CTGTTCTGGA GCGGGACTCA 
GAACAGCAGA GGCTGCAGGA TGAGTTGGAG CTCACCAAAC TCACTCTGGA GAAAGAGCGA 
CTCCACAGCC CAGGCCCGAG CAGCAGAGCA GAACGGGGGC CTAGAGGAGA GCCAGGTGTA 
CAACTGGGAG AGGTCTCGGG AGTGAAGACT GAGCCTAGTC CTGAGATGGA GGAGAGGCAG 
CTCTGGGAAC AGAGGCTTGA GCACCTGCAG CGAGCGGTGG CTCTCTTGGA GGTTGACCGA 
AGCCAGCTGC AACAACATAA CGCCCAGCTG CGGACCACCC TGGAGCAGGT GGAGCGAGAG 
CGGAGGAAAC TGAAGAGGGG TTCCATGCGT GCATTGCGGG CAGGGGACCT GGAGAGCGGT 
GAAGCCACGG CACCATCACC CATGCAACAG GATGGAAGAG GAAGGCAGAA GGGCTGCTCA 
GATGCCAAGC ACATGGTTGA GCTGCAAAAA GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC 
TTGGAGCGGA AGCAGAAGCA GGACTACATT GCTCGCTCAG TTCAGACGAG CCGTGAGCTG 
GCGGACCTGC ACCACGGCCT CTCTCACTCC CTTCTCGCTG TAGCCCAAGC CCCCGAGGCC 
ACCGTCTTAG AGGCCGAGAC CCGCAAGCTG GACGAGTCTC TGACTCAAAG CCTGACATCC 
CCCGGGCCAG CACTGCTGTG CCCCAGCTCC AGTGCTGCTC AGACTGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013840768.1
CDS	109..6825
Translation	MELKAEHVKLSGSLLTCCLRLTVGAPQARESTGSGRLNGSEPSQLMLLLSKTQELEKEAHKRSQELIQLKSQGDLEKAELQDRVTELSALLTQSQKQNEDYEKMVKALRETMKILEANHAELMEHEASLSRNAQEERLSLQQVIKDITQAMVEEGDHMAQGSGHEHSLDLDPSGFFSQFDSQQPDKALTLVRSVLTQRRQAVQDLKQQLSGCQEAVSSLQQQHHQWEEEGEALRQRLQKLTGERDTLAGQTVGLQGEVDSLSRERELLQKTREELQQQLEVLEQEAWRLRRTNMELQLQGDSAQGEKEEQQEELHLAVRERERLQEMLASLEAKQSESLSELITLREALESSRLEGELLRQEQTEVTAVLAKAEQSIVELSSSENSLKAEVADLRAAAVKLSALNEALALDKVGLNQQLLQLEQQNQSVCSRMEAAEQARSALQVGLAEVERSREALWEKNTYLEAQLQKAEAARAELQADLRGVQEEKEEIQEKLSEAHHQQEAVSVQMEQLRQEAKRQEEVLAREVQEKEALVREKAALEVRLQAVERDRQDLSEQLLGLSSAKELLENSLFEAQQQNSLIEVTKGQLEVQIQTITQAKEVIQGEVRCLQLELDTERSRAEQERDAATKQLAQAEQEGQTALQQQKSAHEEEVKGLQEKWEKERAWHQQELDKALKSLEREKTELEMRLREQQAETEAMRTQREEERAEAESTLRQMQLETEKERMSLLETLLQTQKELADASQQLERLRQDMKVQKLKEQETTGMLQTQLREAQKELEQAAQQHRDSLAALQEEGRALLQDKIDLHKQVEDLKSQLVSKEDSQRLVDQEVQEKLREAQECSRIQKELEKEKASLTLLLVEKEQRLLGLQEADSIRQQELSSLRQDMQEAQAGQKELSAQVELLKQEVKAKETDFLAQEARLLEELEASQVTEQQLRASLWAQEAKAAQLQLRLRSTESQLEVLLAEQQPGHQAQAQLASLCAVLQQALGSVCESRPELSGGGDSAPSLWGPEPDQNGARTLFKRGPLLTALSAEAVASALHKLNQDLWKTQQARDDLRDQAQKLGQRLTETEAEKSQLHTELQALQRQLSQNQEERSKWEGKQTSLESELVELHETVTSLQSRLRQAELQGIEAQNEQELLQAAKENLTAQVEHLQAAVAEARAQASAAEVLEEDLKTARSALKLKSKEVESERERAQALQEQGELKVAQGKALQENLARLTQTLSEREGEVETLQGKIQELEKQREMQKAALEVLSLDLKKRNQEVDLQQEQIQELEECRSVLEHLPMAVQEREQKLTLQREQIKELEKDRETQRNILEHQLLELEKKAQVIESQKGQIQDLKKQLVTLECLALELEENHHKIECQQKTIEELEGQREMQRAALTHLTLDLEERSQELQVQGSQIRELESHNSLLARELQEKDQEMTSQQDQTEELQRQKEHLTQDLKRREQEIVLLKERIQVLEDQRTLQTKILEEDLEQIKMSLRERGRDLASQRQLMQERAEEGKGQSKAQRGSLEHMKLILRDKEKEVECQQEHIQELQEHKAQLEQQLQGLHRKMGETSLLLTQREQEIVALQRHLQEAREQGELKEQSLQGQLEEAQRALARRDQELKALQQQQAQGQEENVMEKAGLLQRELEQAHVTLKERQAELEDYKEHVRRLQEELAVEGQRVQALEEVLSDLRAESREQEKALLALQQQCAEQAQEHEAEARALQDSWLQAEAMLKERDQELEALRAEGLSSQHREEAACGQVEALQEALSKAQADLQEKEQHLLGQVELSRSLEASTATLQAALDSCQAQSRQLEEALRKREGEVQVRDLRYQEAMQQLQQALTQRDEELRQQRKQGQLLEKSLAQRGQEDATQEKQEAKHEREEEEIRSLRESLQELQLTLAQKEEEILELREAQQRKSLEDLSHKGCPGKEPATTFDSLGPRLQRELERLQAALKQTEAREIEWREKAQDLALSLAQSKASVSDLQEVALFLQASVLERDSEQQRLQDELELTKLTLEKERLHSPGPSSRAERGPRGEPGVQLGEVSGVKTEPSPEMEERQLWEQRLEHLQRAVALLEVDRSQLQQHNAQLRTTLEQVERERRKLKRGSMRALRAGDLESGEATAPSPMQQDGRGRQKGCSDAKHMVELQKEVALLRAQLALERKQKQDYIARSVQTSRELADLHHGLSHSLLAVAQAPEATVLEAETRKLDESLTQSLTSPGPALLCPSSSAAQTASR

Target ORF information:

RefSeq Version	XM_013985314.1
Organism	Sus scrofa(Pig)
Definition	Sus scrofa centrosomal protein 250kDa (CEP250), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013985314.1

ORF Insert Sequence:

ATGGAGCTCA AGGCTGAGCA CGTGAAGCTC TCTGGGTCCC TGTTGACCTG TTGTCTGCGC 
TTGACTGTGG GAGCACCACA GGCCCGGGAA TCCACTGGAT CTGGGAGGCT GAATGGGAGT 
GAACCATCCC AGCTGATGCT GCTACTGTCT AAGACCCAGG AGCTGGAGAA GGAAGCCCAT 
AAAAGGAGCC AGGAGTTAAT ACAGCTTAAG AGTCAAGGGG ATCTGGAGAA GGCTGAGCTT 
CAGGATCGGG TGACCGAGCT TTCTGCTCTG CTGACCCAGT CTCAGAAGCA GAATGAAGAT 
TATGAAAAGA TGGTAAAGGC TCTGAGAGAG ACAATGAAGA TCCTGGAGGC AAATCATGCA 
GAGTTAATGG AACATGAGGC ATCTCTTAGT AGGAATGCCC AAGAGGAGAG GCTGTCTTTA 
CAGCAAGTGA TCAAGGATAT AACCCAGGCC ATGGTAGAAG AAGGGGACCA TATGGCCCAA 
GGCTCTGGTC ACGAGCACTC CTTGGACTTG GATCCTAGTG GCTTCTTCTC CCAGTTTGAT 
TCCCAGCAGC CAGACAAGGC CCTCACTCTG GTGCGTTCTG TGTTGACTCA GAGACGCCAG 
GCTGTGCAGG ACCTAAAGCA GCAGCTTTCA GGCTGTCAGG AAGCTGTGAG CTCCTTGCAG 
CAGCAGCATC ATCAGTGGGA AGAAGAGGGT GAGGCCTTGA GACAGCGGCT GCAGAAGCTC 
ACCGGGGAGC GGGACACCCT GGCAGGGCAG ACTGTGGGCC TGCAGGGGGA GGTGGACTCT 
CTGAGCAGGG AGCGAGAGCT CCTGCAAAAG ACCAGGGAGG AGCTGCAGCA GCAGCTGGAG 
GTGCTGGAGC AGGAGGCCTG GCGACTACGG AGGACAAACA TGGAGCTTCA GCTGCAGGGC 
GACTCTGCTC AGGGCGAGAA GGAGGAGCAG CAGGAGGAGC TGCACCTGGC TGTCCGAGAA 
CGGGAGCGCC TTCAGGAGAT GCTGGCGAGC CTGGAAGCCA AACAGTCAGA ATCTCTCAGT 
GAACTGATCA CCCTTCGGGA AGCCCTGGAG TCTAGTCGCC TAGAAGGGGA GTTGCTGAGG 
CAAGAGCAAA CAGAAGTGAC TGCTGTGCTA GCAAAGGCAG AACAGTCTAT TGTGGAGCTG 
TCAAGTTCTG AGAACAGCCT GAAGGCTGAG GTAGCTGATC TTCGGGCTGC AGCTGTCAAG 
CTCAGTGCCT TAAATGAGGC TTTGGCCTTA GATAAAGTTG GACTGAACCA GCAGCTTCTC 
CAGTTAGAAC AGCAGAACCA GTCTGTGTGC AGCAGGATGG AGGCAGCAGA GCAGGCAAGA 
AGCGCTTTGC AGGTGGGCCT GGCAGAGGTG GAGAGGAGCA GGGAAGCGCT GTGGGAAAAG 
AACACTTACC TGGAGGCTCA GCTGCAAAAA GCAGAGGCGG CCAGGGCTGA GCTGCAGGCA 
GATCTCAGGG GCGTCCAGGA AGAGAAGGAA GAAATTCAAG AGAAACTAAG CGAGGCACAT 
CACCAGCAGG AGGCAGTCTC AGTTCAGATG GAGCAGCTGC GTCAGGAGGC AAAGCGGCAG 
GAAGAGGTGC TCGCCCGGGA AGTCCAGGAG AAGGAGGCTC TGGTGCGGGA AAAGGCGGCT 
CTTGAAGTGC GGCTGCAGGC TGTGGAGCGG GACCGGCAGG ACCTCTCTGA ACAACTGCTG 
GGGCTCAGCT CGGCCAAGGA GCTTCTGGAG AACAGTCTGT TTGAGGCCCA GCAACAGAAT 
TCTCTGATAG AAGTCACCAA GGGGCAGCTG GAGGTCCAGA TTCAAACTAT CACTCAAGCC 
AAGGAGGTAA TCCAAGGGGA AGTGAGGTGC CTACAGCTGG AACTGGATAC TGAACGGAGC 
CGGGCAGAAC AGGAGCGGGA TGCTGCAACC AAACAGCTGG CCCAGGCTGA GCAAGAGGGG 
CAGACCGCCC TGCAGCAGCA GAAGTCAGCC CATGAGGAGG AAGTGAAGGG GCTCCAGGAA 
AAATGGGAGA AAGAGCGTGC CTGGCACCAG CAGGAACTGG ATAAGGCTCT GAAGAGCCTT 
GAGAGGGAGA AAACGGAGCT GGAAATGAGG CTGAGGGAGC AGCAGGCAGA AACAGAGGCC 
ATGCGGACGC AGAGGGAAGA AGAACGGGCT GAGGCTGAGA GCACCCTGCG CCAGATGCAG 
CTCGAAACAG AGAAGGAGAG AATGTCCCTC CTGGAGACAC TGCTGCAGAC CCAGAAGGAG 
CTGGCAGATG CCAGCCAACA ACTGGAGCGG CTGAGGCAGG ACATGAAGGT TCAGAAGTTA 
AAGGAGCAGG AGACCACTGG GATGCTGCAG ACCCAGCTCC GGGAAGCTCA GAAGGAGCTG 
GAACAGGCAG CCCAGCAGCA CAGAGACAGC CTTGCTGCCC TCCAAGAAGA AGGCCGTGCC 
CTGCTGCAGG ATAAGATAGA CCTGCACAAG CAGGTGGAGG ACTTGAAGTC TCAGCTGGTT 
TCCAAGGAAG ACTCCCAAAG GCTGGTAGAC CAGGAAGTTC AGGAGAAGCT GAGAGAGGCC 
CAGGAATGTA GCCGAATTCA GAAGGAGCTG GAGAAAGAAA AGGCCAGCCT GACTCTGTTG 
CTGGTGGAAA AGGAACAGAG ACTCCTTGGT TTACAAGAGG CCGACTCCAT TCGACAACAA 
GAGCTGAGCT CCCTGCGCCA GGACATGCAG GAGGCCCAGG CAGGGCAGAA AGAGCTCAGC 
GCCCAGGTGG AATTACTGAA GCAGGAGGTG AAGGCAAAGG AGACTGACTT TCTGGCCCAG 
GAAGCGCGGC TGCTGGAAGA GCTGGAGGCA TCTCAAGTAA CAGAGCAGCA GCTACGAGCT 
TCCTTGTGGG CCCAGGAAGC CAAGGCCGCA CAACTACAGC TGCGACTGCG CAGCACAGAG 
AGCCAGCTGG AGGTGCTGCT GGCAGAGCAG CAGCCTGGGC ACCAGGCCCA GGCCCAGCTG 
GCCAGCCTTT GTGCTGTCCT GCAGCAGGCC CTGGGGTCTG TTTGTGAGAG CAGGCCTGAG 
CTGAGTGGCG GGGGAGACTC TGCTCCCTCC CTCTGGGGCC CTGAGCCAGA CCAGAATGGA 
GCTAGGACCC TTTTCAAGAG AGGGCCCCTC CTGACAGCTC TCTCGGCTGA GGCGGTGGCC 
TCTGCCCTTC ACAAGCTTAA CCAAGACCTG TGGAAGACTC AGCAGGCCCG GGATGATCTG 
AGGGATCAGG CCCAGAAGCT GGGACAACGT CTTACTGAAA CAGAGGCCGA GAAGAGCCAG 
CTCCACACAG AGTTGCAGGC TCTGCAGAGG CAGCTCTCCC AGAACCAGGA AGAGAGATCC 
AAGTGGGAAG GAAAACAGAC CTCCTTAGAA TCTGAGCTGG TGGAACTGCA TGAAACTGTG 
ACATCCTTAC AGAGTCGCCT ACGGCAAGCA GAGCTGCAGG GAATAGAAGC CCAGAATGAG 
CAAGAGTTAC TGCAGGCAGC CAAGGAGAAC CTGACAGCCC AGGTGGAACA CCTCCAAGCA 
GCTGTTGCAG AAGCCAGGGC TCAGGCAAGC GCTGCCGAGG TCCTGGAGGA AGACCTCAAA 
ACTGCACGCT CAGCCCTGAA ACTGAAAAGC AAGGAGGTGG AGAGTGAGCG TGAGCGAGCC 
CAGGCCCTAC AGGAGCAGGG CGAGCTGAAG GTGGCCCAAG GGAAAGCTTT GCAGGAGAAT 
TTGGCTCGCC TGACTCAGAC CCTATCTGAG AGAGAAGGGG AGGTGGAAAC TTTGCAGGGA 
AAAATCCAGG AGCTGGAGAA GCAACGGGAA ATGCAAAAGG CTGCTTTGGA GGTGCTGTCT 
TTGGACCTCA AAAAGAGGAA CCAAGAGGTG GATCTGCAGC AAGAACAGAT TCAGGAGCTG 
GAGGAGTGCA GATCGGTTTT AGAGCATCTC CCCATGGCAG TCCAGGAGCG AGAGCAGAAG 
CTGACTCTGC AGAGGGAGCA GATCAAGGAG CTCGAGAAGG ACCGAGAGAC ACAGAGGAAC 
ATCTTGGAGC ATCAGCTTCT GGAACTTGAG AAGAAGGCCC AAGTGATTGA GTCCCAGAAA 
GGACAGATTC AGGATCTGAA GAAGCAGCTG GTTACTCTGG AATGCCTGGC CCTGGAACTA 
GAGGAAAACC ATCACAAAAT AGAGTGCCAG CAAAAGACAA TTGAGGAGCT GGAGGGCCAG 
AGGGAAATGC AGAGGGCAGC TCTGACCCAT CTGACACTGG ACCTGGAGGA AAGGAGCCAG 
GAGCTGCAGG TACAGGGCAG CCAGATCCGG GAGCTAGAGA GTCACAACAG CCTCTTGGCA 
AGAGAGCTCC AGGAGAAGGA CCAGGAGATG ACGTCCCAGC AAGACCAGAC AGAGGAACTG 
CAGAGGCAGA AGGAGCATTT GACACAGGAC CTCAAGAGGC GGGAACAGGA GATAGTGCTG 
CTGAAGGAGA GGATTCAGGT CTTGGAAGAT CAGAGAACCC TACAGACCAA GATCCTAGAG 
GAGGACCTGG AGCAGATCAA AATGTCCTTG AGGGAGCGAG GCCGGGACCT GGCCTCGCAG 
AGGCAGCTGA TGCAGGAGCG GGCAGAGGAA GGGAAGGGCC AGAGTAAAGC ACAGCGCGGG 
AGCCTGGAGC ATATGAAGCT GATCCTGCGT GATAAGGAGA AGGAGGTGGA ATGCCAGCAG 
GAGCATATCC AGGAACTGCA GGAGCACAAG GCCCAGCTGG AGCAGCAGCT CCAGGGCTTA 
CACAGGAAGA TGGGGGAGAC CAGCCTTCTC CTGACCCAGC GGGAGCAGGA GATAGTGGCT 
CTGCAGCGGC ACCTGCAGGA AGCCAGGGAA CAGGGAGAGC TGAAAGAGCA GTCACTTCAA 
GGTCAGCTGG AAGAGGCCCA GAGGGCGCTG GCCCGAAGGG ACCAGGAGCT CAAGGCCCTG 
CAGCAGCAGC AGGCCCAAGG CCAGGAGGAA AATGTGATGG AAAAGGCGGG CTTACTGCAG 
AGAGAGCTGG AGCAGGCCCA CGTGACACTG AAGGAGCGCC AGGCGGAGCT TGAGGATTAC 
AAGGAGCATG TGAGAAGGCT CCAGGAAGAG CTGGCAGTGG AGGGGCAGCG GGTACAGGCC 
CTGGAGGAGG TGTTGAGTGA CCTAAGGGCT GAATCTCGGG AACAAGAGAA GGCCCTGCTG 
GCCCTCCAGC AGCAGTGTGC CGAGCAGGCC CAGGAGCACG AGGCAGAGGC CAGGGCCCTG 
CAGGACAGCT GGCTGCAGGC AGAGGCTATG CTCAAGGAAC GGGACCAGGA ACTGGAGGCC 
CTGCGGGCAG AGGGCCTGTC TTCCCAGCAT CGGGAGGAGG CTGCCTGTGG CCAGGTGGAG 
GCTCTGCAGG AGGCCCTCAG CAAGGCTCAG GCTGACCTGC AGGAGAAAGA GCAGCATCTC 
CTTGGGCAGG TGGAACTGAG CCGCAGCCTA GAGGCCAGCA CGGCCACGTT GCAGGCTGCC 
CTGGACTCCT GCCAGGCACA GTCCCGGCAG CTGGAGGAGG CCCTGAGGAA GCGGGAAGGT 
GAGGTCCAGG TCCGGGACCT CCGATACCAG GAGGCCATGC AGCAGCTCCA GCAGGCACTT 
ACCCAGAGGG ATGAAGAGCT GAGACAACAG AGGAAACAGG GGCAGCTCCT GGAGAAGTCT 
CTGGCCCAGA GGGGCCAAGA GGATGCAACC CAAGAGAAGC AAGAAGCAAA GCACGAGAGA 
GAAGAGGAAG AGATAAGGAG CCTTCGTGAG AGTCTGCAGG AGCTACAGCT GACTCTAGCA 
CAAAAGGAAG AGGAGATCCT GGAGCTGAGG GAGGCCCAGC AGAGGAAGAG TCTGGAGGAC 
TTGTCTCACA AAGGCTGCCC AGGGAAGGAG CCGGCTACAA CATTTGATTC CTTAGGGCCC 
AGGCTGCAGC GGGAACTGGA GCGACTACAG GCAGCACTGA AGCAGACAGA AGCCAGGGAG 
ATCGAGTGGA GGGAGAAGGC CCAGGATCTG GCGCTGTCCC TGGCCCAGAG CAAGGCCAGT 
GTCAGCGATC TGCAGGAGGT AGCCCTGTTC CTGCAAGCAT CTGTTCTGGA GCGGGACTCA 
GAACAGCAGA GGCTGCAGGA TGAGTTGGAG CTCACCAAAC TCACTCTGGA GAAAGAGCGA 
CTCCACAGCC CAGGCCCGAG CAGCAGAGCA GAACGGGGGC CTAGAGGAGA GCCAGGTGTA 
CAACTGGGAG AGGTCTCGGG AGTGAAGACT GAGCCTAGTC CTGAGATGGA GGAGAGGCAG 
CTCTGGGAAC AGAGGCTTGA GCACCTGCAG CGAGCGGTGG CTCTCTTGGA GGTTGACCGA 
AGCCAGCTGC AACAACATAA CGCCCAGCTG CGGACCACCC TGGAGCAGGT GGAGCGAGAG 
CGGAGGAAAC TGAAGAGGGG TTCCATGCGT GCATTGCGGG CAGGGGACCT GGAGAGCGGT 
GAAGCCACGG CACCATCACC CATGCAACAG GATGGAAGAG GAAGGCAGAA GGGCTGCTCA 
GATGCCAAGC ACATGGTTGA GCTGCAAAAA GAGGTGGCCC TCCTGCGAGC CCAGCTGGCC 
TTGGAGCGGA AGCAGAAGCA GGACTACATT GCTCGCTCAG TTCAGACGAG CCGTGAGCTG 
GCGGACCTGC ACCACGGCCT CTCTCACTCC CTTCTCGCTG TAGCCCAAGC CCCCGAGGCC 
ACCGTCTTAG AGGCCGAGAC CCGCAAGCTG GACGAGTCTC TGACTCAAAG CCTGACATCC 
CCCGGGCCAG CACTGCTGTG CCCCAGCTCC AGTGCTGCTC AGACTGCCTC CAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CEP250
Entrez Gene ID	100151840
Full Name	centrosomal protein 250
Gene Type	protein-coding
Organism	Sus scrofa(pig)
Genome	Chromosome: 17 Map Location: 17\|17

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CEP250 cDNA ORF clone, Sus scrofa(pig)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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