ZNF532 cDNA ORF clone, Struthio camelus australis

Gene
Clones
gRNAs

Summary

Gene Symbol	ZNF532
Entrez Gene ID	104139381
Full Name	zinc finger protein 532
Gene Type	protein-coding
Organism	Struthio camelus australis

mRNA and Protein(s)

mRNA	Protein	Name
XM_009667534.1	XP_009665829.1	LOW QUALITY PROTEIN: zinc finger protein 532

Pathways from BioSystems
Gene Ontology

The following ZNF532 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZNF532 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSt63512	XM_009667534.1 Latest version!	Struthio camelus australis zinc finger protein 532 (ZNF532), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZNF532 ( XM_009667534.1 ) cDNA ORF clone, Struthio camelus australis -> XP_009665829.1 Struthio camelus australis zinc finger protein 532 (ZNF532), mRNA.

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CloneID	OSt63512
Clone ID Related Accession (Same CDS sequence)	XM_009667534.1
Accession Version	XM_009667534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-10-14
Organism	Struthio camelus australis
Product	LOW QUALITY PROTEIN: zinc finger protein 532
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_009270033.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Struthio camelus australis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGACCATGG GGGATATGAA GACCCCAGAC TTTGATGACC TTCTTGCAGC CTTTGACATA 
CCAGACATGG TAGATCCAAA AGCAGCTATT GAGTCTGGAC ATGATGATCA TGAAAGCCAC 
ATAAAACAAA ATGCTCACGC AGATGAAGAT TCTCATGCGC CATCATCATC TGATGTTGGT 
GTGAGCGTCA TTGTGAAAAA CGTGCGTACT ATTGATTCTT CTGAAGGAGT GGACAAGGAT 
GGCCACCATT CCACAGGCAA TGGCTTGCAT AATGGTTTTC TAACATCATC AGCTCTTGAT 
AGCTACAGTA AAGATGAAGG GAAGTGTCTC AAAGACGATG GGTGTGCTTC TGAGACTACA 
TTGAAAGACT CGGCTTTCAA TCAGTTTAGC CCAATCTCTA GTGCTGAAGA ATTTGATGAT 
GATGAAAAAA TTGAGGTAGA TGATCCTCCG GATAAAGAAG AGATGCGTGG AAATTTCAGG 
GCAAATGTCT TGACGGGATC TGTATCTCAG CAGNTTGGAG GGGAGAACTT GATTAAAGCT 
GGAATCCCAG TTTCAAGTAA TATGGATAAA AGTAAAGTTG GTAAACGAGA GACAGAGACA 
AACTCTGTGA ATTTAAGTGT CTATGAGCCT TTTAAACCTC GAAAGGTAGA GGACAAATTG 
TTGAAAGACA GTTCTGACAA GCTACTTGAA AACAGGGTAC TTGATGGAAA ACTGAGTGCT 
GAAAAAAGTG AAGCAAATCT TGCTAGCATT TCACAGTCCA AAGCCAAGTC ATCAGCAAAG 
CTTTCTTCGT GCATTGCTGC AATTGCAGCA CTGAGTGCTA AAAAGGCAGC TACAGACACC 
GGTAAGGATT TACAGTCTAA TTCTGGAGAG TCTTCTCCAT TACCAAAAGA CATGAGTGAA 
AGTCCCCGGG CTATTGAAAA ATCACCTGAA CCTCAGAGTC TCATTGATGG TGCTAAAAAA 
CCATCTATCA AACAACCTGA TAGTCCCAGG AGTGTATCAA GTGAAAACAG TAGCAAAGGG 
TCTCCATCTT CTCCTGCAGG GTCGACACCA GCAATTCCTA AAGTTCGAAT AAAAACCATC 
AAGACTTCCT CTGGGGAGAT CAAGAGAACT GTTACTAGAG TGTTGCCAGA AGTTGATTTG 
GACTCTGGTA AAAAGCTAGA GCAGACTGCT TCTATGGTAA CTTCCATGAC ATCTCTCCTG 
TCCTCATCAA CTTCTGCTGC TGTTCTTTCT TCCCCTCCCA GAGCTCCTCT CCAGTCCGCG 
GTTGTCACCA ATGCAGTTGC GTCTGCAGAA CTTGCACCGA AACAGGTCAC TATCAAACCT 
GTGGCTACTG CCTTTCTCCC AGTTTCAGCG GTTAAAACAG CAGGATCACA AGTTATTAAT 
TTGAAGCTTG CTAACAATAC TACAGTGAAA GCCACTGTAA TATCTGCTGC TTCAGTGCAG 
AGTGCCAGTA GCGCCATTAT AAAGGCTGCC AACGCTATAC AGCAGCAGAC TGTTGTGGTG 
CCAGCATCAA GCCTTGCCAG TGCTAAACTT GTGCCAAAGA CGGTCCATCT TGCCAACCTT 
AATCTTCTGC CTCAAGGTGC TCAAACCACC TCTGAACTCC GTCAAGTGCT AACAAAACCT 
CAGCAACAAA TAAAGCAGGC AATAATTTCC GCAGCAGCTA CTCAGCCTTC TAAAAAAGTG 
TCTCGAGTCC AGGTGGTGTC ATCTCTACAG AGTTCTGTAG TGGAAGCATT CAATAAGGTG 
CTGAGCAGTG TCAATCCTGT CCCAGTTTAC ATCCCAAACC TCAGCCCCCC TGCCAATGCC 
GGCATAACGT TACCAACTCG AGGTTACAAG TGTTTGGAGT GCGGCGACTC GTTTGCTCTC 
GAGAAGAGCC TGACCCAGCA CTATGACAGG AGGAGTGTGC GAATTGAAGT GACGTGTAAT 
CATTGTACAA AGAATTTAGT TTTCTACAAT AAGTGCAGTC TTCTTTCGCA TGCTCGTGGA 
CACAAGGAAA AAGGAGTTGT AATGCAGTGT TCCCACTTGA TTTTGAAACC AGTCCCAGCA 
GATCAAATGA TAGCATCTCC TTCAAGCAAT ACTGCCACAT CCTCTCTGCT TCAAAGCCCT 
GTGGGAGTTG GCACGCATAC TGTAACAAAG ATACAGTCTG GCATAACCGG GACAGTAATA 
TCAGCCCCAG CGAGTACACC TATCATTCCA GCTATGCCAC TAGACGAAGA TCCTTCAAAA 
CTTTGTAGAC ATAGTCTAAA GTGTTTGGAG TGTAATGAAG TTTTCCAAGA TGAGACATCG 
CTTGCTACAC ATTTCCAGCA GGCTGCAGAC ACAAGTGGAC AAAAGACATG CAATATCTGC 
CAGATGCTGC TTCCTAACCA GTGCAGTTTT GCATCACACC AGAGAATTCA TCAGCATAAA 
TCTCCATACA CGTGTCCTGA ATGTGGAGCA ATCTGCAGAT CTGTCCACTT CCAGACTCAT 
GTCACTAAGA ACTGCCTGCA TTATACACGA AGAGTTGGTT TTCGCTGCGT ACATTGCAAT 
GTCGTGTACT CTGATGTGGC GGCTCTCAAG TCTCACATTC AAGGTTCTCA CTGTGAAGTG 
TTTTACAAGT GTCCTATCTG TCCCATGGCG TTTAAATCTG CTCCCAGCAC ACACTCCCAT 
GCCTATACAC AACACCCGGG TATCAAGATA GGCGAACCAA AAATAATATA TAAATGCTCT 
ATGTGTGACA CTGTGTTTAC TCTACAACCT TTACTCTATC GCCACTTTGA TCAGCACATT 
GAAAACCAGA AGGTGTCTGT TTTCAAGTGT CCAGACTGTT CCCTGCTATA TGCACAGAAG 
CAGCTTATGA TGGATCATAT AAAGTCTATG CATGGAACTC TGAAAAGTGT TGAGGGGCCA 
CCAAACCTGG GGATAAATTT GCCTCTTAGT ACTAAACCTA CAACTCAGAA CTCAGCCAGT 
CACAACAAAG AGGACGCAAA ATCTGTCAAC GGAACAGAAA AGTTAGAGAA GAAATCTCCT 
TCTCCCATAA AGAAAGCAGA GCCTAAAAAA GTTACTAATC CCGGCTGGAC GTGCTGGGAG 
TGTGATAGGC TCTTCACTCA GAGGGACGTT TACATCTCCC ACATGAGGAA AGAACATGGA 
AAGCAAATGA AGAAACATCC ATGTCGACAA TGTGACAAAT CTTTCAGTTC ATCCCATAGT 
TTATGTCGTC ACAATCGTAT CAAACACAAA GGCATTAGGA AGGTTTATAC ATGCTCGCAC 
TGCCCAGATT CAAGACGTAC TTTTACCAAA CGTTTGATGT TGGAAAAACA TATTCAGCTA 
ATGCATGGCA TTAAAGACCC TGATGTAAAA GAAATGACTG AATCAACCAA TATGGAAGAA 
AGGGAAGTAA AAGAAGATAC AAAGGTTCCT AGTCCAAAGC GTAAATTGGA AGAACCCGTA 
CTGGAGTTCA GGCCTCCGCG AGGTGCAATC ACTCAGCCAT TGAAGAAGCT AAAGATAAAT 
GTTTTTAAAG TTCACAAGTG TGCTGTGTGT GGCTTTACAA CAGAAAATCT TCTCCAGTTT 
CATGAACATA TTCCTCAGCA TAAGTCTGAT GGCTCTTCGT ACCAGTGCAG GGAATGTGGA 
CTCTGCTACA CATCTCACGT GTCTCTCTCC AGACATCTCT TCATCGTACA TAAGCTGAAG 
GAGCCTCAAC CAGTATCAAA ACAAAACGGG TCTGGGGAAG ATAATCAGCA AGAAAACAAA 
CCCAATCATG AGGATGAATC ATCTGACAAT ACGGTATCGG ACAGAAGATG CAAAGTGTGC 
GCAAAGACTT TTGAAACTGA AGCGGCCTTA AATACTCACA TGAGAACCCA TGGCATGGCC 
TTCATTAAGT CAAAAAGAAT GAGTTCAGCT GAAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009665829.1
CDS	196..4074
Translation	MTMGDMKTPDFDDLLAAFDIPDMVDPKAAIESGHDDHESHIKQNAHADEDSHAPSSSDVGVSVIVKNVRTIDSSEGVDKDGHHSTGNGLHNGFLTSSALDSYSKDEGKCLKDDGCASETTLKDSAFNQFSPISSAEEFDDDEKIEVDDPPDKEEMRGNFRANVLTGSVSQQnullGGENLIKAGIPVSSNMDKSKVGKRETETNSVNLSVYEPFKPRKVEDKLLKDSSDKLLENRVLDGKLSAEKSEANLASISQSKAKSSAKLSSCIAAIAALSAKKAATDTGKDLQSNSGESSPLPKDMSESPRAIEKSPEPQSLIDGAKKPSIKQPDSPRSVSSENSSKGSPSSPAGSTPAIPKVRIKTIKTSSGEIKRTVTRVLPEVDLDSGKKLEQTASMVTSMTSLLSSSTSAAVLSSPPRAPLQSAVVTNAVASAELAPKQVTIKPVATAFLPVSAVKTAGSQVINLKLANNTTVKATVISAASVQSASSAIIKAANAIQQQTVVVPASSLASAKLVPKTVHLANLNLLPQGAQTTSELRQVLTKPQQQIKQAIISAAATQPSKKVSRVQVVSSLQSSVVEAFNKVLSSVNPVPVYIPNLSPPANAGITLPTRGYKCLECGDSFALEKSLTQHYDRRSVRIEVTCNHCTKNLVFYNKCSLLSHARGHKEKGVVMQCSHLILKPVPADQMIASPSSNTATSSLLQSPVGVGTHTVTKIQSGITGTVISAPASTPIIPAMPLDEDPSKLCRHSLKCLECNEVFQDETSLATHFQQAADTSGQKTCNICQMLLPNQCSFASHQRIHQHKSPYTCPECGAICRSVHFQTHVTKNCLHYTRRVGFRCVHCNVVYSDVAALKSHIQGSHCEVFYKCPICPMAFKSAPSTHSHAYTQHPGIKIGEPKIIYKCSMCDTVFTLQPLLYRHFDQHIENQKVSVFKCPDCSLLYAQKQLMMDHIKSMHGTLKSVEGPPNLGINLPLSTKPTTQNSASHNKEDAKSVNGTEKLEKKSPSPIKKAEPKKVTNPGWTCWECDRLFTQRDVYISHMRKEHGKQMKKHPCRQCDKSFSSSHSLCRHNRIKHKGIRKVYTCSHCPDSRRTFTKRLMLEKHIQLMHGIKDPDVKEMTESTNMEEREVKEDTKVPSPKRKLEEPVLEFRPPRGAITQPLKKLKINVFKVHKCAVCGFTTENLLQFHEHIPQHKSDGSSYQCRECGLCYTSHVSLSRHLFIVHKLKEPQPVSKQNGSGEDNQQENKPNHEDESSDNTVSDRRCKVCAKTFETEAALNTHMRTHGMAFIKSKRMSSAEK

Target ORF information:

RefSeq Version	XM_009667534.1
Organism	Struthio camelus australis
Definition	Struthio camelus australis zinc finger protein 532 (ZNF532), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009667534.1

ORF Insert Sequence:

ATGACCATGG GGGATATGAA GACCCCAGAC TTTGATGACC TTCTTGCAGC CTTTGACATA 
CCAGACATGG TAGATCCAAA AGCAGCTATT GAGTCTGGAC ATGATGATCA TGAAAGCCAC 
ATAAAACAAA ATGCTCACGC AGATGAAGAT TCTCATGCGC CATCATCATC TGATGTTGGT 
GTGAGCGTCA TTGTGAAAAA CGTGCGTACT ATTGATTCTT CTGAAGGAGT GGACAAGGAT 
GGCCACCATT CCACAGGCAA TGGCTTGCAT AATGGTTTTC TAACATCATC AGCTCTTGAT 
AGCTACAGTA AAGATGAAGG GAAGTGTCTC AAAGACGATG GGTGTGCTTC TGAGACTACA 
TTGAAAGACT CGGCTTTCAA TCAGTTTAGC CCAATCTCTA GTGCTGAAGA ATTTGATGAT 
GATGAAAAAA TTGAGGTAGA TGATCCTCCG GATAAAGAAG AGATGCGTGG AAATTTCAGG 
GCAAATGTCT TGACGGGATC TGTATCTCAG CAGNTTGGAG GGGAGAACTT GATTAAAGCT 
GGAATCCCAG TTTCAAGTAA TATGGATAAA AGTAAAGTTG GTAAACGAGA GACAGAGACA 
AACTCTGTGA ATTTAAGTGT CTATGAGCCT TTTAAACCTC GAAAGGTAGA GGACAAATTG 
TTGAAAGACA GTTCTGACAA GCTACTTGAA AACAGGGTAC TTGATGGAAA ACTGAGTGCT 
GAAAAAAGTG AAGCAAATCT TGCTAGCATT TCACAGTCCA AAGCCAAGTC ATCAGCAAAG 
CTTTCTTCGT GCATTGCTGC AATTGCAGCA CTGAGTGCTA AAAAGGCAGC TACAGACACC 
GGTAAGGATT TACAGTCTAA TTCTGGAGAG TCTTCTCCAT TACCAAAAGA CATGAGTGAA 
AGTCCCCGGG CTATTGAAAA ATCACCTGAA CCTCAGAGTC TCATTGATGG TGCTAAAAAA 
CCATCTATCA AACAACCTGA TAGTCCCAGG AGTGTATCAA GTGAAAACAG TAGCAAAGGG 
TCTCCATCTT CTCCTGCAGG GTCGACACCA GCAATTCCTA AAGTTCGAAT AAAAACCATC 
AAGACTTCCT CTGGGGAGAT CAAGAGAACT GTTACTAGAG TGTTGCCAGA AGTTGATTTG 
GACTCTGGTA AAAAGCTAGA GCAGACTGCT TCTATGGTAA CTTCCATGAC ATCTCTCCTG 
TCCTCATCAA CTTCTGCTGC TGTTCTTTCT TCCCCTCCCA GAGCTCCTCT CCAGTCCGCG 
GTTGTCACCA ATGCAGTTGC GTCTGCAGAA CTTGCACCGA AACAGGTCAC TATCAAACCT 
GTGGCTACTG CCTTTCTCCC AGTTTCAGCG GTTAAAACAG CAGGATCACA AGTTATTAAT 
TTGAAGCTTG CTAACAATAC TACAGTGAAA GCCACTGTAA TATCTGCTGC TTCAGTGCAG 
AGTGCCAGTA GCGCCATTAT AAAGGCTGCC AACGCTATAC AGCAGCAGAC TGTTGTGGTG 
CCAGCATCAA GCCTTGCCAG TGCTAAACTT GTGCCAAAGA CGGTCCATCT TGCCAACCTT 
AATCTTCTGC CTCAAGGTGC TCAAACCACC TCTGAACTCC GTCAAGTGCT AACAAAACCT 
CAGCAACAAA TAAAGCAGGC AATAATTTCC GCAGCAGCTA CTCAGCCTTC TAAAAAAGTG 
TCTCGAGTCC AGGTGGTGTC ATCTCTACAG AGTTCTGTAG TGGAAGCATT CAATAAGGTG 
CTGAGCAGTG TCAATCCTGT CCCAGTTTAC ATCCCAAACC TCAGCCCCCC TGCCAATGCC 
GGCATAACGT TACCAACTCG AGGTTACAAG TGTTTGGAGT GCGGCGACTC GTTTGCTCTC 
GAGAAGAGCC TGACCCAGCA CTATGACAGG AGGAGTGTGC GAATTGAAGT GACGTGTAAT 
CATTGTACAA AGAATTTAGT TTTCTACAAT AAGTGCAGTC TTCTTTCGCA TGCTCGTGGA 
CACAAGGAAA AAGGAGTTGT AATGCAGTGT TCCCACTTGA TTTTGAAACC AGTCCCAGCA 
GATCAAATGA TAGCATCTCC TTCAAGCAAT ACTGCCACAT CCTCTCTGCT TCAAAGCCCT 
GTGGGAGTTG GCACGCATAC TGTAACAAAG ATACAGTCTG GCATAACCGG GACAGTAATA 
TCAGCCCCAG CGAGTACACC TATCATTCCA GCTATGCCAC TAGACGAAGA TCCTTCAAAA 
CTTTGTAGAC ATAGTCTAAA GTGTTTGGAG TGTAATGAAG TTTTCCAAGA TGAGACATCG 
CTTGCTACAC ATTTCCAGCA GGCTGCAGAC ACAAGTGGAC AAAAGACATG CAATATCTGC 
CAGATGCTGC TTCCTAACCA GTGCAGTTTT GCATCACACC AGAGAATTCA TCAGCATAAA 
TCTCCATACA CGTGTCCTGA ATGTGGAGCA ATCTGCAGAT CTGTCCACTT CCAGACTCAT 
GTCACTAAGA ACTGCCTGCA TTATACACGA AGAGTTGGTT TTCGCTGCGT ACATTGCAAT 
GTCGTGTACT CTGATGTGGC GGCTCTCAAG TCTCACATTC AAGGTTCTCA CTGTGAAGTG 
TTTTACAAGT GTCCTATCTG TCCCATGGCG TTTAAATCTG CTCCCAGCAC ACACTCCCAT 
GCCTATACAC AACACCCGGG TATCAAGATA GGCGAACCAA AAATAATATA TAAATGCTCT 
ATGTGTGACA CTGTGTTTAC TCTACAACCT TTACTCTATC GCCACTTTGA TCAGCACATT 
GAAAACCAGA AGGTGTCTGT TTTCAAGTGT CCAGACTGTT CCCTGCTATA TGCACAGAAG 
CAGCTTATGA TGGATCATAT AAAGTCTATG CATGGAACTC TGAAAAGTGT TGAGGGGCCA 
CCAAACCTGG GGATAAATTT GCCTCTTAGT ACTAAACCTA CAACTCAGAA CTCAGCCAGT 
CACAACAAAG AGGACGCAAA ATCTGTCAAC GGAACAGAAA AGTTAGAGAA GAAATCTCCT 
TCTCCCATAA AGAAAGCAGA GCCTAAAAAA GTTACTAATC CCGGCTGGAC GTGCTGGGAG 
TGTGATAGGC TCTTCACTCA GAGGGACGTT TACATCTCCC ACATGAGGAA AGAACATGGA 
AAGCAAATGA AGAAACATCC ATGTCGACAA TGTGACAAAT CTTTCAGTTC ATCCCATAGT 
TTATGTCGTC ACAATCGTAT CAAACACAAA GGCATTAGGA AGGTTTATAC ATGCTCGCAC 
TGCCCAGATT CAAGACGTAC TTTTACCAAA CGTTTGATGT TGGAAAAACA TATTCAGCTA 
ATGCATGGCA TTAAAGACCC TGATGTAAAA GAAATGACTG AATCAACCAA TATGGAAGAA 
AGGGAAGTAA AAGAAGATAC AAAGGTTCCT AGTCCAAAGC GTAAATTGGA AGAACCCGTA 
CTGGAGTTCA GGCCTCCGCG AGGTGCAATC ACTCAGCCAT TGAAGAAGCT AAAGATAAAT 
GTTTTTAAAG TTCACAAGTG TGCTGTGTGT GGCTTTACAA CAGAAAATCT TCTCCAGTTT 
CATGAACATA TTCCTCAGCA TAAGTCTGAT GGCTCTTCGT ACCAGTGCAG GGAATGTGGA 
CTCTGCTACA CATCTCACGT GTCTCTCTCC AGACATCTCT TCATCGTACA TAAGCTGAAG 
GAGCCTCAAC CAGTATCAAA ACAAAACGGG TCTGGGGAAG ATAATCAGCA AGAAAACAAA 
CCCAATCATG AGGATGAATC ATCTGACAAT ACGGTATCGG ACAGAAGATG CAAAGTGTGC 
GCAAAGACTT TTGAAACTGA AGCGGCCTTA AATACTCACA TGAGAACCCA TGGCATGGCC 
TTCATTAAGT CAAAAAGAAT GAGTTCAGCT GAAAAGTGA