mphosph8 cDNA ORF clone, Sinocyclocheilus grahami

Gene
Clones
gRNAs

Summary

Gene Symbol	mphosph8
Entrez Gene ID	107597386
Full Name	M-phase phosphoprotein 8
Gene Type	protein-coding
Organism	Sinocyclocheilus grahami

mRNA and Protein(s)

mRNA	Protein	Name
XM_016288416.1	XP_016143902.1	M-phase phosphoprotein 8 isoform X2
XM_016288415.1	XP_016143901.1	M-phase phosphoprotein 8 isoform X1

Pathways from BioSystems
Gene Ontology

The following mphosph8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mphosph8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi28763	XM_016288415.1 Latest version!	Sinocyclocheilus grahami M-phase phosphoprotein 8 (mphosph8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OSi28764	XM_016288416.1 Latest version!	Sinocyclocheilus grahami M-phase phosphoprotein 8 (mphosph8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mphosph8 ( XM_016288415.1 ) cDNA ORF clone, Sinocyclocheilus grahami -> XP_016143901.1 Sinocyclocheilus grahami M-phase phosphoprotein 8 (mphosph8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSi28763
Clone ID Related Accession (Same CDS sequence)	XM_016288415.1
Accession Version	XM_016288415.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-18
Organism	Sinocyclocheilus grahami
Product	M-phase phosphoprotein 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015505410.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus grahami Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACCG GAGCCGAGAG AGCGGAGCCT GGAGACAGCG AACAGGATGA AGAAGATGTA 
TATGAAGTGG AGAGGATTAT TGATACGCGA GTGGTGGAGG GAGAGGTGCT GTACCGGGTG 
CGATGGAAGA ACTACTCATC GGACGATGAC ACCTGGGAGC CTGAGGCACA CTTGGACGAC 
TGCAGAGAGG TGCTGCTTGC CTACAAGAAG GCCCTGGCTG AGATGAAGCC AAAGAAGGAA 
ACTGGCTTGA AGCTGCCCAT GAAGAGTGAT TTGTTTGATG CTGATTCTGA GAGTGACAGT 
GATATAGAAA AACAGAAAGA ATCACCAATA AAGAAGAAGA AAAAGAAGAA AGTGGAGGAA 
TCAGATGATG AGATGCCTGT GAAGGAAAAG AAGAAGAAAA AAAAGAAGGA GAAATGGTGG 
GAAGACAAAC CTCTGCCTGC ACCGGAGTCT GATGAGGAGG AGGCAGAGAG CAGGGATCCT 
TCCCCTGCAC CTCCTAAAAA AGACACAAAG AAGAGGCTCA TTGACTCTGA TGATGACGAT 
GATGCCCCAG TTACTCCCAA GAAACAAAAG AAGCTTGACA AGCATAAAGA TGGTGGAAAA 
CAGAAGAAAG AGAGTGGAGA GGACCACAAG AAAAAGAAAG TAAAGTCAAA AAAAGAGATT 
GATACCTCAG ATGAAGAGGA TGAGAAGAGC GACGTGCCCT CGGAGTCCCA CACGGATGAT 
ACAACAAACA CAGAAACAAA CGATTCCTTC AAAACCATGA CAGCAAAAGC CACAGACAAA 
TCTGCAAGAA GTGAGAGTGG AGGTGATCTC AAGCAGGCCA AGCAGAAGAA GGCCAAGTCT 
GACCTGAAGC TGCAAGGTTT CAAAGACCTG ATTCAAGAGA AGAAACCTAA AAAAGTGGAG 
GTTTCAACGG CCACACCTAA AGAAAGTGGT GCAAACAAGC ACAAGAGCTT TACTAGTAGC 
AAGAGCACTA GCAAGTCATC TCGAAGCGAG GAGGAGCCCG ACTCCAGTGA CACGGGGGCA 
GCTACGTCCG CACCCAAGTC AAAGGTTAAA AGCAAAGGGC AGGAAGCAGG CCCACCTTCT 
CAGAAGCTTT CATCTGCCTC TTCCTCCTCC TCATCCTCCA CAGCATCAAC AGCCCCCATC 
AAGCCCAAAG AGGAGGAGCC GAAGGAAGAA GTTGGTGAAA AGGGAGGTGC CTCTAACTTG 
TTTGAGAAGT TCCTGTTGAA CTGTGAGGCA AAAGATCGTG TACCAAGGAG ACAAGCGGAC 
CAGAACAAGA ATATAACTCC AAAGGGTGCA GGGAAAAATG ACAAGAAAAC AAAATTGTCC 
AAGCAGTCTC CTGCACGGAA ACCTGATTCA GACAAGACAG AGAAGGCAAA AGATGTGCCT 
CGACCTGGTC AGAGTCCCTT CTCTATGGAG ATCGAGGAGA AACAGGAGAA GGGAACATCC 
GAGAAAAGTG ATAAATCAGA CGAACTGGCC CCCAAGTCTA AGGAGGAATT ACAAAGAGAG 
CAGAGAGAGG AAGAGCAAAG GAAGAGGAAA GAAAAGATGG AAGAGGAGGA GAGAAAGAGG 
AAAGAAAGGA TTGAAGAAGA GGAGAGAAAG AAGAAGGAGA GAATAGAGGA AGCCCAACGG 
GAGAGAAAAG CACGCATGGA GGAGGCGCAG AGGCTTGCAG AGGAGGCCCG GCAAGAGCGG 
CTGGAAAGGA AGTCCATGAC TGAGTCAATA GCATCTCCTG ACTCGACAGA AGACTCCAGA 
TGGAAGGACA AGAGGAGGAG AAGGAGGGAT GACAGTGAAT CACGGGTCTT CACCGCCTGC 
GATGACAATC AGGACTCTCG GGAGACCATG GAGCGCTCTG ACAAAACTCC TGACAGGGGA 
CCGCCATCTC TTAATCTTGG GGTGGAGCTC AAGCTGGATT GGATGACACT GGAGGACTTT 
CAGAAACACT TGAATGGAGA GGATGAGAAT CTCTCTCCGC TAGCCTTAAC TCCTGCGGAG 
CTGCGAGAAG CAGTGAAGAA TGGGAATTAC CTGGCGGTAA AACGTGCACT TAGTTCCAAA 
GAGGACTACA ATCTGGACCA GGAGGACTCT AGCGGCATGT CCTTAACACT GTTGGCTGCT 
GCAGGCGGTC AGGATGACGT CCTCAGGCTG CTCATTAAGA AAGGAGTAAA GGTTAACGCT 
AGACAGAAGA ACGGCACCAC TGCCCTGATG CATGCTGCTG AAAAGAACTT CTTGACAACA 
GTGGCCATTC TCCTAGAGGC AGGATCATCT GTAAATGCCC AAACTCTGGG TGGAGAAACC 
GCTCTCATGA AGGCTTGCAA GAGGGGCAAT GCAGATGTGG TTCGCCTCCT GCTGGAATAT 
GGCGCTGACT GCAACATCTT GTCTAAACAT AAGAACACTG CCTTGTACTA TGCCAAACTT 
AGCAACAATT TGATGATATA TGATCTCATC AAAGACCATA TGCAAACGTT ATCAACTGTG 
GCGGAGGAGA CAATCAGAGC ATATTTTGAA ACGCGGCTGG CCCTACTGGA ACCTGTATTC 
CCTTTGGCTT GCCATAGATT GTGCGAAGGG CCAGACTTTT CACTGGAATT CAACTACAAA 
CCCCCTCAAC ACACACCCGG AGAAGGATCT GGCATCCTGC TTTTTATCTT CCATGCAAAC 
TTCCTTAATG AGATCACAGC TAGACTCTGT GGGCCCTGTA GTGTTCATGC TGTCGTCCTG 
AATGATAAGT TTCAGCTGCC CATCTTCTTG GACAGCCATT TCATATACTC TTTCAGTCCT 
GTTCAAGGAA CCAACAAACT GTTCATCCGT CTGGCAGAGT CGCCCACAGC TAAGGTTAAA 
CTCCTAATTT GTGCATACAG AGTCCAACTA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016143901.1
CDS	212..3067
Translation	MTTGAERAEPGDSEQDEEDVYEVERIIDTRVVEGEVLYRVRWKNYSSDDDTWEPEAHLDDCREVLLAYKKALAEMKPKKETGLKLPMKSDLFDADSESDSDIEKQKESPIKKKKKKKVEESDDEMPVKEKKKKKKKEKWWEDKPLPAPESDEEEAESRDPSPAPPKKDTKKRLIDSDDDDDAPVTPKKQKKLDKHKDGGKQKKESGEDHKKKKVKSKKEIDTSDEEDEKSDVPSESHTDDTTNTETNDSFKTMTAKATDKSARSESGGDLKQAKQKKAKSDLKLQGFKDLIQEKKPKKVEVSTATPKESGANKHKSFTSSKSTSKSSRSEEEPDSSDTGAATSAPKSKVKSKGQEAGPPSQKLSSASSSSSSSTASTAPIKPKEEEPKEEVGEKGGASNLFEKFLLNCEAKDRVPRRQADQNKNITPKGAGKNDKKTKLSKQSPARKPDSDKTEKAKDVPRPGQSPFSMEIEEKQEKGTSEKSDKSDELAPKSKEELQREQREEEQRKRKEKMEEEERKRKERIEEEERKKKERIEEAQRERKARMEEAQRLAEEARQERLERKSMTESIASPDSTEDSRWKDKRRRRRDDSESRVFTACDDNQDSRETMERSDKTPDRGPPSLNLGVELKLDWMTLEDFQKHLNGEDENLSPLALTPAELREAVKNGNYLAVKRALSSKEDYNLDQEDSSGMSLTLLAAAGGQDDVLRLLIKKGVKVNARQKNGTTALMHAAEKNFLTTVAILLEAGSSVNAQTLGGETALMKACKRGNADVVRLLLEYGADCNILSKHKNTALYYAKLSNNLMIYDLIKDHMQTLSTVAEETIRAYFETRLALLEPVFPLACHRLCEGPDFSLEFNYKPPQHTPGEGSGILLFIFHANFLNEITARLCGPCSVHAVVLNDKFQLPIFLDSHFIYSFSPVQGTNKLFIRLAESPTAKVKLLICAYRVQLQ

Target ORF information:

RefSeq Version	XM_016288415.1
Organism	Sinocyclocheilus grahami
Definition	Sinocyclocheilus grahami M-phase phosphoprotein 8 (mphosph8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016288415.1

ORF Insert Sequence:

ATGACTACCG GAGCCGAGAG AGCGGAGCCT GGAGACAGCG AACAGGATGA AGAAGATGTA 
TATGAAGTGG AGAGGATTAT TGATACGCGA GTGGTGGAGG GAGAGGTGCT GTACCGGGTG 
CGATGGAAGA ACTACTCATC GGACGATGAC ACCTGGGAGC CTGAGGCACA CTTGGACGAC 
TGCAGAGAGG TGCTGCTTGC CTACAAGAAG GCCCTGGCTG AGATGAAGCC AAAGAAGGAA 
ACTGGCTTGA AGCTGCCCAT GAAGAGTGAT TTGTTTGATG CTGATTCTGA GAGTGACAGT 
GATATAGAAA AACAGAAAGA ATCACCAATA AAGAAGAAGA AAAAGAAGAA AGTGGAGGAA 
TCAGATGATG AGATGCCTGT GAAGGAAAAG AAGAAGAAAA AAAAGAAGGA GAAATGGTGG 
GAAGACAAAC CTCTGCCTGC ACCGGAGTCT GATGAGGAGG AGGCAGAGAG CAGGGATCCT 
TCCCCTGCAC CTCCTAAAAA AGACACAAAG AAGAGGCTCA TTGACTCTGA TGATGACGAT 
GATGCCCCAG TTACTCCCAA GAAACAAAAG AAGCTTGACA AGCATAAAGA TGGTGGAAAA 
CAGAAGAAAG AGAGTGGAGA GGACCACAAG AAAAAGAAAG TAAAGTCAAA AAAAGAGATT 
GATACCTCAG ATGAAGAGGA TGAGAAGAGC GACGTGCCCT CGGAGTCCCA CACGGATGAT 
ACAACAAACA CAGAAACAAA CGATTCCTTC AAAACCATGA CAGCAAAAGC CACAGACAAA 
TCTGCAAGAA GTGAGAGTGG AGGTGATCTC AAGCAGGCCA AGCAGAAGAA GGCCAAGTCT 
GACCTGAAGC TGCAAGGTTT CAAAGACCTG ATTCAAGAGA AGAAACCTAA AAAAGTGGAG 
GTTTCAACGG CCACACCTAA AGAAAGTGGT GCAAACAAGC ACAAGAGCTT TACTAGTAGC 
AAGAGCACTA GCAAGTCATC TCGAAGCGAG GAGGAGCCCG ACTCCAGTGA CACGGGGGCA 
GCTACGTCCG CACCCAAGTC AAAGGTTAAA AGCAAAGGGC AGGAAGCAGG CCCACCTTCT 
CAGAAGCTTT CATCTGCCTC TTCCTCCTCC TCATCCTCCA CAGCATCAAC AGCCCCCATC 
AAGCCCAAAG AGGAGGAGCC GAAGGAAGAA GTTGGTGAAA AGGGAGGTGC CTCTAACTTG 
TTTGAGAAGT TCCTGTTGAA CTGTGAGGCA AAAGATCGTG TACCAAGGAG ACAAGCGGAC 
CAGAACAAGA ATATAACTCC AAAGGGTGCA GGGAAAAATG ACAAGAAAAC AAAATTGTCC 
AAGCAGTCTC CTGCACGGAA ACCTGATTCA GACAAGACAG AGAAGGCAAA AGATGTGCCT 
CGACCTGGTC AGAGTCCCTT CTCTATGGAG ATCGAGGAGA AACAGGAGAA GGGAACATCC 
GAGAAAAGTG ATAAATCAGA CGAACTGGCC CCCAAGTCTA AGGAGGAATT ACAAAGAGAG 
CAGAGAGAGG AAGAGCAAAG GAAGAGGAAA GAAAAGATGG AAGAGGAGGA GAGAAAGAGG 
AAAGAAAGGA TTGAAGAAGA GGAGAGAAAG AAGAAGGAGA GAATAGAGGA AGCCCAACGG 
GAGAGAAAAG CACGCATGGA GGAGGCGCAG AGGCTTGCAG AGGAGGCCCG GCAAGAGCGG 
CTGGAAAGGA AGTCCATGAC TGAGTCAATA GCATCTCCTG ACTCGACAGA AGACTCCAGA 
TGGAAGGACA AGAGGAGGAG AAGGAGGGAT GACAGTGAAT CACGGGTCTT CACCGCCTGC 
GATGACAATC AGGACTCTCG GGAGACCATG GAGCGCTCTG ACAAAACTCC TGACAGGGGA 
CCGCCATCTC TTAATCTTGG GGTGGAGCTC AAGCTGGATT GGATGACACT GGAGGACTTT 
CAGAAACACT TGAATGGAGA GGATGAGAAT CTCTCTCCGC TAGCCTTAAC TCCTGCGGAG 
CTGCGAGAAG CAGTGAAGAA TGGGAATTAC CTGGCGGTAA AACGTGCACT TAGTTCCAAA 
GAGGACTACA ATCTGGACCA GGAGGACTCT AGCGGCATGT CCTTAACACT GTTGGCTGCT 
GCAGGCGGTC AGGATGACGT CCTCAGGCTG CTCATTAAGA AAGGAGTAAA GGTTAACGCT 
AGACAGAAGA ACGGCACCAC TGCCCTGATG CATGCTGCTG AAAAGAACTT CTTGACAACA 
GTGGCCATTC TCCTAGAGGC AGGATCATCT GTAAATGCCC AAACTCTGGG TGGAGAAACC 
GCTCTCATGA AGGCTTGCAA GAGGGGCAAT GCAGATGTGG TTCGCCTCCT GCTGGAATAT 
GGCGCTGACT GCAACATCTT GTCTAAACAT AAGAACACTG CCTTGTACTA TGCCAAACTT 
AGCAACAATT TGATGATATA TGATCTCATC AAAGACCATA TGCAAACGTT ATCAACTGTG 
GCGGAGGAGA CAATCAGAGC ATATTTTGAA ACGCGGCTGG CCCTACTGGA ACCTGTATTC 
CCTTTGGCTT GCCATAGATT GTGCGAAGGG CCAGACTTTT CACTGGAATT CAACTACAAA 
CCCCCTCAAC ACACACCCGG AGAAGGATCT GGCATCCTGC TTTTTATCTT CCATGCAAAC 
TTCCTTAATG AGATCACAGC TAGACTCTGT GGGCCCTGTA GTGTTCATGC TGTCGTCCTG 
AATGATAAGT TTCAGCTGCC CATCTTCTTG GACAGCCATT TCATATACTC TTTCAGTCCT 
GTTCAAGGAA CCAACAAACT GTTCATCCGT CTGGCAGAGT CGCCCACAGC TAAGGTTAAA 
CTCCTAATTT GTGCATACAG AGTCCAACTA CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mphosph8 ( XM_016288416.1 ) cDNA ORF clone, Sinocyclocheilus grahami -> XP_016143902.1 Sinocyclocheilus grahami M-phase phosphoprotein 8 (mphosph8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSi28764
Clone ID Related Accession (Same CDS sequence)	XM_016288416.1
Accession Version	XM_016288416.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-18
Organism	Sinocyclocheilus grahami
Product	M-phase phosphoprotein 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015505410.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus grahami Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACCG GAGCCGAGAG AGCGGAGCCT GGAGACAGCG AACAGGATGA AGAAGATGTA 
TATGAAGTGG AGAGGATTAT TGATACGCGA GTGGTGGAGG GAGAGGTGCT GTACCGGGTG 
CGATGGAAGA ACTACTCATC GGACGATGAC ACCTGGGAGC CTGAGGCACA CTTGGACGAC 
TGCAGAGAGG TGCTGCTTGC CTACAAGAAG GCCCTGGCTG AGATGAAGCC AAAGAAGGAA 
ACTGGCTTGC TGCCCATGAA GAGTGATTTG TTTGATGCTG ATTCTGAGAG TGACAGTGAT 
ATAGAAAAAC AGAAAGAATC ACCAATAAAG AAGAAGAAAA AGAAGAAAGT GGAGGAATCA 
GATGATGAGA TGCCTGTGAA GGAAAAGAAG AAGAAAAAAA AGAAGGAGAA ATGGTGGGAA 
GACAAACCTC TGCCTGCACC GGAGTCTGAT GAGGAGGAGG CAGAGAGCAG GGATCCTTCC 
CCTGCACCTC CTAAAAAAGA CACAAAGAAG AGGCTCATTG ACTCTGATGA TGACGATGAT 
GCCCCAGTTA CTCCCAAGAA ACAAAAGAAG CTTGACAAGC ATAAAGATGG TGGAAAACAG 
AAGAAAGAGA GTGGAGAGGA CCACAAGAAA AAGAAAGTAA AGTCAAAAAA AGAGATTGAT 
ACCTCAGATG AAGAGGATGA GAAGAGCGAC GTGCCCTCGG AGTCCCACAC GGATGATACA 
ACAAACACAG AAACAAACGA TTCCTTCAAA ACCATGACAG CAAAAGCCAC AGACAAATCT 
GCAAGAAGTG AGAGTGGAGG TGATCTCAAG CAGGCCAAGC AGAAGAAGGC CAAGTCTGAC 
CTGAAGCTGC AAGGTTTCAA AGACCTGATT CAAGAGAAGA AACCTAAAAA AGTGGAGGTT 
TCAACGGCCA CACCTAAAGA AAGTGGTGCA AACAAGCACA AGAGCTTTAC TAGTAGCAAG 
AGCACTAGCA AGTCATCTCG AAGCGAGGAG GAGCCCGACT CCAGTGACAC GGGGGCAGCT 
ACGTCCGCAC CCAAGTCAAA GGTTAAAAGC AAAGGGCAGG AAGCAGGCCC ACCTTCTCAG 
AAGCTTTCAT CTGCCTCTTC CTCCTCCTCA TCCTCCACAG CATCAACAGC CCCCATCAAG 
CCCAAAGAGG AGGAGCCGAA GGAAGAAGTT GGTGAAAAGG GAGGTGCCTC TAACTTGTTT 
GAGAAGTTCC TGTTGAACTG TGAGGCAAAA GATCGTGTAC CAAGGAGACA AGCGGACCAG 
AACAAGAATA TAACTCCAAA GGGTGCAGGG AAAAATGACA AGAAAACAAA ATTGTCCAAG 
CAGTCTCCTG CACGGAAACC TGATTCAGAC AAGACAGAGA AGGCAAAAGA TGTGCCTCGA 
CCTGGTCAGA GTCCCTTCTC TATGGAGATC GAGGAGAAAC AGGAGAAGGG AACATCCGAG 
AAAAGTGATA AATCAGACGA ACTGGCCCCC AAGTCTAAGG AGGAATTACA AAGAGAGCAG 
AGAGAGGAAG AGCAAAGGAA GAGGAAAGAA AAGATGGAAG AGGAGGAGAG AAAGAGGAAA 
GAAAGGATTG AAGAAGAGGA GAGAAAGAAG AAGGAGAGAA TAGAGGAAGC CCAACGGGAG 
AGAAAAGCAC GCATGGAGGA GGCGCAGAGG CTTGCAGAGG AGGCCCGGCA AGAGCGGCTG 
GAAAGGAAGT CCATGACTGA GTCAATAGCA TCTCCTGACT CGACAGAAGA CTCCAGATGG 
AAGGACAAGA GGAGGAGAAG GAGGGATGAC AGTGAATCAC GGGTCTTCAC CGCCTGCGAT 
GACAATCAGG ACTCTCGGGA GACCATGGAG CGCTCTGACA AAACTCCTGA CAGGGGACCG 
CCATCTCTTA ATCTTGGGGT GGAGCTCAAG CTGGATTGGA TGACACTGGA GGACTTTCAG 
AAACACTTGA ATGGAGAGGA TGAGAATCTC TCTCCGCTAG CCTTAACTCC TGCGGAGCTG 
CGAGAAGCAG TGAAGAATGG GAATTACCTG GCGGTAAAAC GTGCACTTAG TTCCAAAGAG 
GACTACAATC TGGACCAGGA GGACTCTAGC GGCATGTCCT TAACACTGTT GGCTGCTGCA 
GGCGGTCAGG ATGACGTCCT CAGGCTGCTC ATTAAGAAAG GAGTAAAGGT TAACGCTAGA 
CAGAAGAACG GCACCACTGC CCTGATGCAT GCTGCTGAAA AGAACTTCTT GACAACAGTG 
GCCATTCTCC TAGAGGCAGG ATCATCTGTA AATGCCCAAA CTCTGGGTGG AGAAACCGCT 
CTCATGAAGG CTTGCAAGAG GGGCAATGCA GATGTGGTTC GCCTCCTGCT GGAATATGGC 
GCTGACTGCA ACATCTTGTC TAAACATAAG AACACTGCCT TGTACTATGC CAAACTTAGC 
AACAATTTGA TGATATATGA TCTCATCAAA GACCATATGC AAACGTTATC AACTGTGGCG 
GAGGAGACAA TCAGAGCATA TTTTGAAACG CGGCTGGCCC TACTGGAACC TGTATTCCCT 
TTGGCTTGCC ATAGATTGTG CGAAGGGCCA GACTTTTCAC TGGAATTCAA CTACAAACCC 
CCTCAACACA CACCCGGAGA AGGATCTGGC ATCCTGCTTT TTATCTTCCA TGCAAACTTC 
CTTAATGAGA TCACAGCTAG ACTCTGTGGG CCCTGTAGTG TTCATGCTGT CGTCCTGAAT 
GATAAGTTTC AGCTGCCCAT CTTCTTGGAC AGCCATTTCA TATACTCTTT CAGTCCTGTT 
CAAGGAACCA ACAAACTGTT CATCCGTCTG GCAGAGTCGC CCACAGCTAA GGTTAAACTC 
CTAATTTGTG CATACAGAGT CCAACTACAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016143902.1
CDS	210..3062
Translation	MTTGAERAEPGDSEQDEEDVYEVERIIDTRVVEGEVLYRVRWKNYSSDDDTWEPEAHLDDCREVLLAYKKALAEMKPKKETGLLPMKSDLFDADSESDSDIEKQKESPIKKKKKKKVEESDDEMPVKEKKKKKKKEKWWEDKPLPAPESDEEEAESRDPSPAPPKKDTKKRLIDSDDDDDAPVTPKKQKKLDKHKDGGKQKKESGEDHKKKKVKSKKEIDTSDEEDEKSDVPSESHTDDTTNTETNDSFKTMTAKATDKSARSESGGDLKQAKQKKAKSDLKLQGFKDLIQEKKPKKVEVSTATPKESGANKHKSFTSSKSTSKSSRSEEEPDSSDTGAATSAPKSKVKSKGQEAGPPSQKLSSASSSSSSSTASTAPIKPKEEEPKEEVGEKGGASNLFEKFLLNCEAKDRVPRRQADQNKNITPKGAGKNDKKTKLSKQSPARKPDSDKTEKAKDVPRPGQSPFSMEIEEKQEKGTSEKSDKSDELAPKSKEELQREQREEEQRKRKEKMEEEERKRKERIEEEERKKKERIEEAQRERKARMEEAQRLAEEARQERLERKSMTESIASPDSTEDSRWKDKRRRRRDDSESRVFTACDDNQDSRETMERSDKTPDRGPPSLNLGVELKLDWMTLEDFQKHLNGEDENLSPLALTPAELREAVKNGNYLAVKRALSSKEDYNLDQEDSSGMSLTLLAAAGGQDDVLRLLIKKGVKVNARQKNGTTALMHAAEKNFLTTVAILLEAGSSVNAQTLGGETALMKACKRGNADVVRLLLEYGADCNILSKHKNTALYYAKLSNNLMIYDLIKDHMQTLSTVAEETIRAYFETRLALLEPVFPLACHRLCEGPDFSLEFNYKPPQHTPGEGSGILLFIFHANFLNEITARLCGPCSVHAVVLNDKFQLPIFLDSHFIYSFSPVQGTNKLFIRLAESPTAKVKLLICAYRVQLQ

Target ORF information:

RefSeq Version	XM_016288416.1
Organism	Sinocyclocheilus grahami
Definition	Sinocyclocheilus grahami M-phase phosphoprotein 8 (mphosph8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016288416.1

ORF Insert Sequence:

ATGACTACCG GAGCCGAGAG AGCGGAGCCT GGAGACAGCG AACAGGATGA AGAAGATGTA 
TATGAAGTGG AGAGGATTAT TGATACGCGA GTGGTGGAGG GAGAGGTGCT GTACCGGGTG 
CGATGGAAGA ACTACTCATC GGACGATGAC ACCTGGGAGC CTGAGGCACA CTTGGACGAC 
TGCAGAGAGG TGCTGCTTGC CTACAAGAAG GCCCTGGCTG AGATGAAGCC AAAGAAGGAA 
ACTGGCTTGC TGCCCATGAA GAGTGATTTG TTTGATGCTG ATTCTGAGAG TGACAGTGAT 
ATAGAAAAAC AGAAAGAATC ACCAATAAAG AAGAAGAAAA AGAAGAAAGT GGAGGAATCA 
GATGATGAGA TGCCTGTGAA GGAAAAGAAG AAGAAAAAAA AGAAGGAGAA ATGGTGGGAA 
GACAAACCTC TGCCTGCACC GGAGTCTGAT GAGGAGGAGG CAGAGAGCAG GGATCCTTCC 
CCTGCACCTC CTAAAAAAGA CACAAAGAAG AGGCTCATTG ACTCTGATGA TGACGATGAT 
GCCCCAGTTA CTCCCAAGAA ACAAAAGAAG CTTGACAAGC ATAAAGATGG TGGAAAACAG 
AAGAAAGAGA GTGGAGAGGA CCACAAGAAA AAGAAAGTAA AGTCAAAAAA AGAGATTGAT 
ACCTCAGATG AAGAGGATGA GAAGAGCGAC GTGCCCTCGG AGTCCCACAC GGATGATACA 
ACAAACACAG AAACAAACGA TTCCTTCAAA ACCATGACAG CAAAAGCCAC AGACAAATCT 
GCAAGAAGTG AGAGTGGAGG TGATCTCAAG CAGGCCAAGC AGAAGAAGGC CAAGTCTGAC 
CTGAAGCTGC AAGGTTTCAA AGACCTGATT CAAGAGAAGA AACCTAAAAA AGTGGAGGTT 
TCAACGGCCA CACCTAAAGA AAGTGGTGCA AACAAGCACA AGAGCTTTAC TAGTAGCAAG 
AGCACTAGCA AGTCATCTCG AAGCGAGGAG GAGCCCGACT CCAGTGACAC GGGGGCAGCT 
ACGTCCGCAC CCAAGTCAAA GGTTAAAAGC AAAGGGCAGG AAGCAGGCCC ACCTTCTCAG 
AAGCTTTCAT CTGCCTCTTC CTCCTCCTCA TCCTCCACAG CATCAACAGC CCCCATCAAG 
CCCAAAGAGG AGGAGCCGAA GGAAGAAGTT GGTGAAAAGG GAGGTGCCTC TAACTTGTTT 
GAGAAGTTCC TGTTGAACTG TGAGGCAAAA GATCGTGTAC CAAGGAGACA AGCGGACCAG 
AACAAGAATA TAACTCCAAA GGGTGCAGGG AAAAATGACA AGAAAACAAA ATTGTCCAAG 
CAGTCTCCTG CACGGAAACC TGATTCAGAC AAGACAGAGA AGGCAAAAGA TGTGCCTCGA 
CCTGGTCAGA GTCCCTTCTC TATGGAGATC GAGGAGAAAC AGGAGAAGGG AACATCCGAG 
AAAAGTGATA AATCAGACGA ACTGGCCCCC AAGTCTAAGG AGGAATTACA AAGAGAGCAG 
AGAGAGGAAG AGCAAAGGAA GAGGAAAGAA AAGATGGAAG AGGAGGAGAG AAAGAGGAAA 
GAAAGGATTG AAGAAGAGGA GAGAAAGAAG AAGGAGAGAA TAGAGGAAGC CCAACGGGAG 
AGAAAAGCAC GCATGGAGGA GGCGCAGAGG CTTGCAGAGG AGGCCCGGCA AGAGCGGCTG 
GAAAGGAAGT CCATGACTGA GTCAATAGCA TCTCCTGACT CGACAGAAGA CTCCAGATGG 
AAGGACAAGA GGAGGAGAAG GAGGGATGAC AGTGAATCAC GGGTCTTCAC CGCCTGCGAT 
GACAATCAGG ACTCTCGGGA GACCATGGAG CGCTCTGACA AAACTCCTGA CAGGGGACCG 
CCATCTCTTA ATCTTGGGGT GGAGCTCAAG CTGGATTGGA TGACACTGGA GGACTTTCAG 
AAACACTTGA ATGGAGAGGA TGAGAATCTC TCTCCGCTAG CCTTAACTCC TGCGGAGCTG 
CGAGAAGCAG TGAAGAATGG GAATTACCTG GCGGTAAAAC GTGCACTTAG TTCCAAAGAG 
GACTACAATC TGGACCAGGA GGACTCTAGC GGCATGTCCT TAACACTGTT GGCTGCTGCA 
GGCGGTCAGG ATGACGTCCT CAGGCTGCTC ATTAAGAAAG GAGTAAAGGT TAACGCTAGA 
CAGAAGAACG GCACCACTGC CCTGATGCAT GCTGCTGAAA AGAACTTCTT GACAACAGTG 
GCCATTCTCC TAGAGGCAGG ATCATCTGTA AATGCCCAAA CTCTGGGTGG AGAAACCGCT 
CTCATGAAGG CTTGCAAGAG GGGCAATGCA GATGTGGTTC GCCTCCTGCT GGAATATGGC 
GCTGACTGCA ACATCTTGTC TAAACATAAG AACACTGCCT TGTACTATGC CAAACTTAGC 
AACAATTTGA TGATATATGA TCTCATCAAA GACCATATGC AAACGTTATC AACTGTGGCG 
GAGGAGACAA TCAGAGCATA TTTTGAAACG CGGCTGGCCC TACTGGAACC TGTATTCCCT 
TTGGCTTGCC ATAGATTGTG CGAAGGGCCA GACTTTTCAC TGGAATTCAA CTACAAACCC 
CCTCAACACA CACCCGGAGA AGGATCTGGC ATCCTGCTTT TTATCTTCCA TGCAAACTTC 
CTTAATGAGA TCACAGCTAG ACTCTGTGGG CCCTGTAGTG TTCATGCTGT CGTCCTGAAT 
GATAAGTTTC AGCTGCCCAT CTTCTTGGAC AGCCATTTCA TATACTCTTT CAGTCCTGTT 
CAAGGAACCA ACAAACTGTT CATCCGTCTG GCAGAGTCGC CCACAGCTAA GGTTAAACTC 
CTAATTTGTG CATACAGAGT CCAACTACAG TGA