bmp2k cDNA ORF clone, Sinocyclocheilus grahami

Gene
Clones
gRNAs

Summary

Gene Symbol	bmp2k
Entrez Gene ID	107567466
Full Name	BMP2 inducible kinase
Gene Type	protein-coding
Organism	Sinocyclocheilus grahami

mRNA and Protein(s)

mRNA	Protein	Name
XM_016253149.1	XP_016108635.1	BMP-2-inducible protein kinase isoform X2
XM_016253148.1	XP_016108634.1	BMP-2-inducible protein kinase isoform X1

Pathways from BioSystems
Gene Ontology

The following bmp2k gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bmp2k cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi53957	XM_016253148.1 Latest version!	Sinocyclocheilus grahami BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OSi53958	XM_016253149.1 Latest version!	Sinocyclocheilus grahami BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bmp2k ( XM_016253148.1 ) cDNA ORF clone, Sinocyclocheilus grahami -> XP_016108634.1 Sinocyclocheilus grahami BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSi53957
Clone ID Related Accession (Same CDS sequence)	XM_016253148.1
Accession Version	XM_016253148.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-18
Organism	Sinocyclocheilus grahami
Product	BMP-2-inducible protein kinase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015506340.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus grahami Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTCGCGTAT GCCGAAATCA GAGAGCGGAA GTCTCGGGGG TGGGTCTGGA 
TCTGGTACTG GAAGCAGCAA CTATATCGGG AAGGTGTTTG CTGTCGGTCG ATACCAAGTG 
ACGGTGGAAG AGCTGATCGC TGAAGGAGGT TTCTCCATGG TGTTTCTGGC CCGCACACAC 
AGTGGAGTAC GATGTGCCCT GAAAAGGATG TATGTGAACA ATGTCCACGA CCTGAACATC 
TTCAAGCGGG AGATCACAAT CATGAAAGAG TTGTCGGGCC ACAAGAACAT TGTGCGGTAT 
CTGGACTCAA CCACCAATGC AGTTGGAGAC AGTGTATGGG AGGTTCTGAT TCTGATGGAG 
TATTGCAAAG CTGGTCAGGT GGTGAAGCAG ATGAATCAAC GGCTGCATAT AGGATTCACA 
GAAGCGGAGG TGCTCAACAT ATTCTGTGAC GCATGTGAAG CTGTTGCTAG ACTCCACCAG 
TGTAAAACTT CAATCATTCA CAGAGACCTA AAGGTAGAAA ACATCCTCCT AAATGACCAG 
GGAAACTACG TCTTGTGTGA CTTTGGAAGT GCCACACATA AAGTTCTGCT TCCACATAAA 
GATGGAATAG CAGTGGTGGA GGATGAGATC AAGAAGTACA CGACTCTGTC ATACCGTGCC 
CCAGAGATGA TTAACTTGTA CCAAGGGAAA GCCATCACCA CAAAGGCTGA TATCTGGGCA 
CTTGGATGCT TGTTGTACAA GCTGTGTTTC TTCACACTTC CATTTGGGGA GAGTCAAGTT 
GCCATATGTG ACGGAACCTT TGCTGTTCCG GACAGTTCAA AATTCTCCTC CAGGTTGCAC 
GGTTTAGTCA GATACATGTT GGAGCCTGAT CAAGAAAAGA GACCTGACAT CTATCAAGTT 
TCTTACTTTG CCTTTCGGTT AGCTGGAAAG GAGTGTCCAG TGCCAAATCT CTTTAACTCT 
CCCCTTCCCA CCTCGTTGCC AGAGCCCCTT ACTGCTAGTG ATATTGCTGC TAAAAAGAGC 
CTGACAAAAG CCAGAATAAC AGATTCAATT GGACCAACAG AAACATCAAT CGCACCCAGG 
CAAAGACCCA AAGCTGCAAA TAGTAATGTT TTACCTTTGT CCAGCTCTGT AACTCCTGTG 
AAAATGACTG GATCCTCGGG TACGGATAGC AACGGCCAGA AAGCTTCTGG AAACGGGCAG 
CAGTCAGCTG CACAGATGCC AAGCAGCAGT CAACAGAACC GGGTGTTACA GCAGCTGCAG 
CCTGGAGACC TTCGACTGCA ACAGCTACAT CATCAACAAC AACAACAGCA GCAGCAGCAC 
AATATACACC ATCAACAGCA AGTCACTGCA CAGCAGCTAC AGTACCTGCA ACAGTATCAT 
CAGGCCATGC AACAGAGCCT TCAGATGCAG CAACAGCATA TGCTTCAGCA GCAGATGATG 
ATGCAGCAGC CCATATATCA ATCCCAGCAG CAACAGCAGG CTCACTACAC TGCCTTGATG 
CATCAGTACC AGCAGGCCTT TGTTCAGCAG CAGCAGCAGC AATGTGCTCC AGTCCAACAG 
GTGCCCCCTC TGGAGTTCCA GATCCCACTG GGCTCCTATA ACCCTGCCGG ACCTTCTCCA 
GTAGACACCC CCTTTGCTAA CATCAGGAAC CCAGTGTCCA CTATGGATGT TATCAGCCCT 
CCTCCCCAGA CTGTCAATAA CCCTCCTGAT ATGTCTGGTT GGAACCCATT TGGAGAAGAC 
AATTTCTCCA AACTCACAGA AGAGGAGCTT CTCGATCGAG AGTTCGACCT TCTAAGAGCA 
AAAAAGCCGG TAGAGCGATC TCGCAGCATG GAGGCCAGCA GCATAGACAG ACAGCAGCCG 
GTACTGCAGC CCTCACTCCC TGAGGATCCA TTTGGCTCTG TGCCGTTCCT GGCCAACGCA 
GCAGGAGCTG GTGTGCTGAC AGCAGAGCAT CCAGTTGAGC AGCTCAGTGT TGCTGTGACT 
GTTCCAAGCA CGCAAGACAC AACCCTGCAA TCAGTCAAAG AACACAAACC TGCAAAGAAA 
AGTAGCTCCA CTAGTTCACT GCCTCAGAAA GCAGGCGAGG AATCAGACAG TGATTTTGAG 
TCGGACCCAC CCTCTCCCAA AAGCAGTGAA GACGAAGAGG CCGAAGAGGA GGAAGGTCTC 
AACAGCGAAC GTGAAGACAC CGAACCTGAG AACCTGGGTC AGAGGCCGTT ACTCATGGAC 
TCTGAGGAAG AGGAGGACAA ACACAGCTCA GACTCAGATT GTGACTCTAG GAAAGCAAAA 
CTTGGGTTGT CGAAGGAAAA GGCTGCACTT TTTCCTCCTA GAACAGTGAA CGCTGAATCT 
GGACCCAGTG TGATCACTCC TCCCAATTCG CCAAGTGTAG CCATGCCTGT CGCGAGCATT 
GTGGATGTGT TTGGTGCTGT TCCTTTCGTT GGCAGTGGTA AGGCCAGAGT GTCACCAGAG 
AGCTCAGATA TTTTTGCCCA AGCCCCTTTT AGGCAGGCTA GCCAGGAGAA CGCTGCGGAA 
GAGACTGACA TATTCATCAA AGCTCCTTTC AACAGAAACC TCTCCAGGTC CAGCAGGAGT 
GGCGATGGAC CCAGTGGCCA GACGCCACCC GTTTCTCCTG ACAGCGTGGA TGTATTCGGA 
TTCTCGCCTT TCCAACCTAG CCACGTAATG AACAGGGATT CTCGGAATCA GGAGGACATC 
TTTGGCCAAG CACCTTTCGA CGAAGCAGAC AGTCCCCAGC AACAGAGGCA GAAGCAGTGC 
AGTCTTCAGA AACTCTCCTC GCGGCAAAGG CGCACCAAGC AAAAGGCCAG TGGCGGGAAT 
GGGAAGCGAC ACCACGGCAC GCCCACTGGT GGCAGGAAGA GCAACAAACC CAGTTTCCGT 
ACCCCCGAAC GGGTGCGAAG GCACAAGAAA GTGGGCAGAC GGGACTCGCA GAGCAGCAAT 
GAGTTCCTCA GCACGTCTGA CTCCAAAGAG AACATCAGTA TTTCCCTGGG CGAGGTGATG 
GAAAAAGAAG CTGCGTTGCC TTCGGAGGAG GTCTTGTTGG ATCCGTTTGG AGCCAAGCCT 
TTTCATCCAC AAGATGGCAG TCGACATGGC CAGCACCAGA GCTTGGGAGA CAACAAAAGT 
GAGATCAATT CGACCAACGG TAGGCCGAGA ACCAGTTCCC TACATGGCGT GTTTGAGGAA 
AATAAGATAG ATGACTTTGG AGCTGTACCT TTCACAGAAC TAGTGGTCGA AAGCGGAGCT 
CCGCAAACCC CACAAAAGGA TCTCGACCCT TTCGGAGCTG CACCTTTCCC CTCAAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016108634.1
CDS	894..4196
Translation	MKKFSRMPKSESGSLGGGSGSGTGSSNYIGKVFAVGRYQVTVEELIAEGGFSMVFLARTHSGVRCALKRMYVNNVHDLNIFKREITIMKELSGHKNIVRYLDSTTNAVGDSVWEVLILMEYCKAGQVVKQMNQRLHIGFTEAEVLNIFCDACEAVARLHQCKTSIIHRDLKVENILLNDQGNYVLCDFGSATHKVLLPHKDGIAVVEDEIKKYTTLSYRAPEMINLYQGKAITTKADIWALGCLLYKLCFFTLPFGESQVAICDGTFAVPDSSKFSSRLHGLVRYMLEPDQEKRPDIYQVSYFAFRLAGKECPVPNLFNSPLPTSLPEPLTASDIAAKKSLTKARITDSIGPTETSIAPRQRPKAANSNVLPLSSSVTPVKMTGSSGTDSNGQKASGNGQQSAAQMPSSSQQNRVLQQLQPGDLRLQQLHHQQQQQQQQHNIHHQQQVTAQQLQYLQQYHQAMQQSLQMQQQHMLQQQMMMQQPIYQSQQQQQAHYTALMHQYQQAFVQQQQQQCAPVQQVPPLEFQIPLGSYNPAGPSPVDTPFANIRNPVSTMDVISPPPQTVNNPPDMSGWNPFGEDNFSKLTEEELLDREFDLLRAKKPVERSRSMEASSIDRQQPVLQPSLPEDPFGSVPFLANAAGAGVLTAEHPVEQLSVAVTVPSTQDTTLQSVKEHKPAKKSSSTSSLPQKAGEESDSDFESDPPSPKSSEDEEAEEEEGLNSEREDTEPENLGQRPLLMDSEEEEDKHSSDSDCDSRKAKLGLSKEKAALFPPRTVNAESGPSVITPPNSPSVAMPVASIVDVFGAVPFVGSGKARVSPESSDIFAQAPFRQASQENAAEETDIFIKAPFNRNLSRSSRSGDGPSGQTPPVSPDSVDVFGFSPFQPSHVMNRDSRNQEDIFGQAPFDEADSPQQQRQKQCSLQKLSSRQRRTKQKASGGNGKRHHGTPTGGRKSNKPSFRTPERVRRHKKVGRRDSQSSNEFLSTSDSKENISISLGEVMEKEAALPSEEVLLDPFGAKPFHPQDGSRHGQHQSLGDNKSEINSTNGRPRTSSLHGVFEENKIDDFGAVPFTELVVESGAPQTPQKDLDPFGAAPFPSKQ

Target ORF information:

RefSeq Version	XM_016253148.1
Organism	Sinocyclocheilus grahami
Definition	Sinocyclocheilus grahami BMP2 inducible kinase (bmp2k), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016253148.1

ORF Insert Sequence:

ATGAAGAAGT TTTCGCGTAT GCCGAAATCA GAGAGCGGAA GTCTCGGGGG TGGGTCTGGA 
TCTGGTACTG GAAGCAGCAA CTATATCGGG AAGGTGTTTG CTGTCGGTCG ATACCAAGTG 
ACGGTGGAAG AGCTGATCGC TGAAGGAGGT TTCTCCATGG TGTTTCTGGC CCGCACACAC 
AGTGGAGTAC GATGTGCCCT GAAAAGGATG TATGTGAACA ATGTCCACGA CCTGAACATC 
TTCAAGCGGG AGATCACAAT CATGAAAGAG TTGTCGGGCC ACAAGAACAT TGTGCGGTAT 
CTGGACTCAA CCACCAATGC AGTTGGAGAC AGTGTATGGG AGGTTCTGAT TCTGATGGAG 
TATTGCAAAG CTGGTCAGGT GGTGAAGCAG ATGAATCAAC GGCTGCATAT AGGATTCACA 
GAAGCGGAGG TGCTCAACAT ATTCTGTGAC GCATGTGAAG CTGTTGCTAG ACTCCACCAG 
TGTAAAACTT CAATCATTCA CAGAGACCTA AAGGTAGAAA ACATCCTCCT AAATGACCAG 
GGAAACTACG TCTTGTGTGA CTTTGGAAGT GCCACACATA AAGTTCTGCT TCCACATAAA 
GATGGAATAG CAGTGGTGGA GGATGAGATC AAGAAGTACA CGACTCTGTC ATACCGTGCC 
CCAGAGATGA TTAACTTGTA CCAAGGGAAA GCCATCACCA CAAAGGCTGA TATCTGGGCA 
CTTGGATGCT TGTTGTACAA GCTGTGTTTC TTCACACTTC CATTTGGGGA GAGTCAAGTT 
GCCATATGTG ACGGAACCTT TGCTGTTCCG GACAGTTCAA AATTCTCCTC CAGGTTGCAC 
GGTTTAGTCA GATACATGTT GGAGCCTGAT CAAGAAAAGA GACCTGACAT CTATCAAGTT 
TCTTACTTTG CCTTTCGGTT AGCTGGAAAG GAGTGTCCAG TGCCAAATCT CTTTAACTCT 
CCCCTTCCCA CCTCGTTGCC AGAGCCCCTT ACTGCTAGTG ATATTGCTGC TAAAAAGAGC 
CTGACAAAAG CCAGAATAAC AGATTCAATT GGACCAACAG AAACATCAAT CGCACCCAGG 
CAAAGACCCA AAGCTGCAAA TAGTAATGTT TTACCTTTGT CCAGCTCTGT AACTCCTGTG 
AAAATGACTG GATCCTCGGG TACGGATAGC AACGGCCAGA AAGCTTCTGG AAACGGGCAG 
CAGTCAGCTG CACAGATGCC AAGCAGCAGT CAACAGAACC GGGTGTTACA GCAGCTGCAG 
CCTGGAGACC TTCGACTGCA ACAGCTACAT CATCAACAAC AACAACAGCA GCAGCAGCAC 
AATATACACC ATCAACAGCA AGTCACTGCA CAGCAGCTAC AGTACCTGCA ACAGTATCAT 
CAGGCCATGC AACAGAGCCT TCAGATGCAG CAACAGCATA TGCTTCAGCA GCAGATGATG 
ATGCAGCAGC CCATATATCA ATCCCAGCAG CAACAGCAGG CTCACTACAC TGCCTTGATG 
CATCAGTACC AGCAGGCCTT TGTTCAGCAG CAGCAGCAGC AATGTGCTCC AGTCCAACAG 
GTGCCCCCTC TGGAGTTCCA GATCCCACTG GGCTCCTATA ACCCTGCCGG ACCTTCTCCA 
GTAGACACCC CCTTTGCTAA CATCAGGAAC CCAGTGTCCA CTATGGATGT TATCAGCCCT 
CCTCCCCAGA CTGTCAATAA CCCTCCTGAT ATGTCTGGTT GGAACCCATT TGGAGAAGAC 
AATTTCTCCA AACTCACAGA AGAGGAGCTT CTCGATCGAG AGTTCGACCT TCTAAGAGCA 
AAAAAGCCGG TAGAGCGATC TCGCAGCATG GAGGCCAGCA GCATAGACAG ACAGCAGCCG 
GTACTGCAGC CCTCACTCCC TGAGGATCCA TTTGGCTCTG TGCCGTTCCT GGCCAACGCA 
GCAGGAGCTG GTGTGCTGAC AGCAGAGCAT CCAGTTGAGC AGCTCAGTGT TGCTGTGACT 
GTTCCAAGCA CGCAAGACAC AACCCTGCAA TCAGTCAAAG AACACAAACC TGCAAAGAAA 
AGTAGCTCCA CTAGTTCACT GCCTCAGAAA GCAGGCGAGG AATCAGACAG TGATTTTGAG 
TCGGACCCAC CCTCTCCCAA AAGCAGTGAA GACGAAGAGG CCGAAGAGGA GGAAGGTCTC 
AACAGCGAAC GTGAAGACAC CGAACCTGAG AACCTGGGTC AGAGGCCGTT ACTCATGGAC 
TCTGAGGAAG AGGAGGACAA ACACAGCTCA GACTCAGATT GTGACTCTAG GAAAGCAAAA 
CTTGGGTTGT CGAAGGAAAA GGCTGCACTT TTTCCTCCTA GAACAGTGAA CGCTGAATCT 
GGACCCAGTG TGATCACTCC TCCCAATTCG CCAAGTGTAG CCATGCCTGT CGCGAGCATT 
GTGGATGTGT TTGGTGCTGT TCCTTTCGTT GGCAGTGGTA AGGCCAGAGT GTCACCAGAG 
AGCTCAGATA TTTTTGCCCA AGCCCCTTTT AGGCAGGCTA GCCAGGAGAA CGCTGCGGAA 
GAGACTGACA TATTCATCAA AGCTCCTTTC AACAGAAACC TCTCCAGGTC CAGCAGGAGT 
GGCGATGGAC CCAGTGGCCA GACGCCACCC GTTTCTCCTG ACAGCGTGGA TGTATTCGGA 
TTCTCGCCTT TCCAACCTAG CCACGTAATG AACAGGGATT CTCGGAATCA GGAGGACATC 
TTTGGCCAAG CACCTTTCGA CGAAGCAGAC AGTCCCCAGC AACAGAGGCA GAAGCAGTGC 
AGTCTTCAGA AACTCTCCTC GCGGCAAAGG CGCACCAAGC AAAAGGCCAG TGGCGGGAAT 
GGGAAGCGAC ACCACGGCAC GCCCACTGGT GGCAGGAAGA GCAACAAACC CAGTTTCCGT 
ACCCCCGAAC GGGTGCGAAG GCACAAGAAA GTGGGCAGAC GGGACTCGCA GAGCAGCAAT 
GAGTTCCTCA GCACGTCTGA CTCCAAAGAG AACATCAGTA TTTCCCTGGG CGAGGTGATG 
GAAAAAGAAG CTGCGTTGCC TTCGGAGGAG GTCTTGTTGG ATCCGTTTGG AGCCAAGCCT 
TTTCATCCAC AAGATGGCAG TCGACATGGC CAGCACCAGA GCTTGGGAGA CAACAAAAGT 
GAGATCAATT CGACCAACGG TAGGCCGAGA ACCAGTTCCC TACATGGCGT GTTTGAGGAA 
AATAAGATAG ATGACTTTGG AGCTGTACCT TTCACAGAAC TAGTGGTCGA AAGCGGAGCT 
CCGCAAACCC CACAAAAGGA TCTCGACCCT TTCGGAGCTG CACCTTTCCC CTCAAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bmp2k ( XM_016253149.1 ) cDNA ORF clone, Sinocyclocheilus grahami -> XP_016108635.1 Sinocyclocheilus grahami BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSi53958
Clone ID Related Accession (Same CDS sequence)	XM_016253149.1
Accession Version	XM_016253149.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-04-18
Organism	Sinocyclocheilus grahami
Product	BMP-2-inducible protein kinase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015506340.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus grahami Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAGT TTTCGCGTAT GCCGAAATCA GAGAGCGGAA GTCTCGGGGG TGGGTCTGGA 
TCTGGTACTG GAAGCAGCAA CTATATCGGG AAGGTGTTTG CTGTCGGTCG ATACCAAGTG 
ACGGTGGAAG AGCTGATCGC TGAAGGAGGT TTCTCCATGG TGTTTCTGGC CCGCACACAC 
AGTGGAGTAC GATGTGCCCT GAAAAGGATG TATGTGAACA ATGTCCACGA CCTGAACATC 
TTCAAGCGGG AGATCACAAT CATGAAAGAG TTGTCGGGCC ACAAGAACAT TGTGCGGTAT 
CTGGACTCAA CCACCAATGC AGTTGGAGAC AGTGTATGGG AGGTTCTGAT TCTGATGGAG 
TATTGCAAAG CTGGTCAGGT GGTGAAGCAG ATGAATCAAC GGCTGCATAT AGGATTCACA 
GAAGCGGAGG TGCTCAACAT ATTCTGTGAC GCATGTGAAG CTGTTGCTAG ACTCCACCAG 
TGTAAAACTT CAATCATTCA CAGAGACCTA AAGGTAGAAA ACATCCTCCT AAATGACCAG 
GGAAACTACG TCTTGTGTGA CTTTGGAAGT GCCACACATA AAGTTCTGCT TCCACATAAA 
GATGGAATAG CAGTGGTGGA GGATGAGATC AAGAAGTACA CGACTCTGTC ATACCGTGCC 
CCAGAGATGA TTAACTTGTA CCAAGGGAAA GCCATCACCA CAAAGGCTGA TATCTGGGCA 
CTTGGATGCT TGTTGTACAA GCTGTGTTTC TTCACACTTC CATTTGGGGA GAGTCAAGTT 
GCCATATGTG ACGGAACCTT TGCTGTTCCG GACAGTTCAA AATTCTCCTC CAGGTTGCAC 
GGTTTAGTCA GATACATGTT GGAGCCTGAT CAAGAAAAGA GACCTGACAT CTATCAAGTT 
TCTTACTTTG CCTTTCGGTT AGCTGGAAAG GAGTGTCCAG TGCCAAATCT CTTTAACTCT 
CCCCTTCCCA CCTCGTTGCC AGAGCCCCTT ACTGCTAGTG ATATTGCTGC TAAAAAGAGC 
CTGACAAAAG CCAGAATAAC AGATTCAATT GGACCAACAG AAACATCAAT CGCACCCAGG 
CAAAGACCCA AAGCTGCAAA TAGTAATGTT TTACCTTTGT CCAGCTCTGT AACTCCTGTG 
AAAATGACTG GATCCTCGGG TACGGATAGC AACGGCCAGA AAGCTTCTGG AAACGGGCAG 
CAGTCAGCTG CACAGATGCC AAGCAGCAGT CAACAGAACC GGGTGTTACA GCAGCTGCAG 
CCTGGAGACC TTCGACTGCA ACAGCTACAT CATCAACAAC AACAACAGCA GCAGCAGCAC 
AATATACACC ATCAACAGCA AGTCACTGCA CAGCAGCTAC AGTACCTGCA ACAGTATCAT 
CAGGCCATGC AACAGAGCCT TCAGATGCAG CAACAGCATA TGCTTCAGCA GCAGATGATG 
ATGCAGCAGC CCATATATCA ATCCCAGCAG CAACAGCAGG CTCACTACAC TGCCTTGATG 
CATCAGTACC AGCAGGCCTT TGTTCAGCAG CAGCAGCAGC AATGTGCTCC AGTCCAACAG 
GTGCCCCCTC TGGAGTTCCA GATCCCACTG GGCTCCTATA ACCCTGCCGG ACCTTCTCCA 
GTAGACACCC CCTTTGCTAA CATCAGGAAC CCAGTGTCCA CTATGGATGT TATCAGCCCT 
CCTCCCCAGA CTGTCAATAA CCCTCCTGAT ATGTCTGGTT GGAACCCATT TGGAGAAGAC 
AATTTCTCCA AACTCACAGA AGAGGAGCTT CTCGATCGAG AGTTCGACCT TCTAAGAGCA 
AAAAAGCCGG TAGAGCGATC TCGCAGCATG GAGGCCAGCA GCATAGACAG ACAGCAGCCG 
GTACTGCAGC CCTCACTCCC TGAGGATCCA TTTGGCTCTG TGCCGTTCCT GGCCAACGCA 
GGAGCTGGTG TGCTGACAGC AGAGCATCCA GTTGAGCAGC TCAGTGTTGC TGTGACTGTT 
CCAAGCACGC AAGACACAAC CCTGCAATCA GTCAAAGAAC ACAAACCTGC AAAGAAAAGT 
AGCTCCACTA GTTCACTGCC TCAGAAAGCA GGCGAGGAAT CAGACAGTGA TTTTGAGTCG 
GACCCACCCT CTCCCAAAAG CAGTGAAGAC GAAGAGGCCG AAGAGGAGGA AGGTCTCAAC 
AGCGAACGTG AAGACACCGA ACCTGAGAAC CTGGGTCAGA GGCCGTTACT CATGGACTCT 
GAGGAAGAGG AGGACAAACA CAGCTCAGAC TCAGATTGTG ACTCTAGGAA AGCAAAACTT 
GGGTTGTCGA AGGAAAAGGC TGCACTTTTT CCTCCTAGAA CAGTGAACGC TGAATCTGGA 
CCCAGTGTGA TCACTCCTCC CAATTCGCCA AGTGTAGCCA TGCCTGTCGC GAGCATTGTG 
GATGTGTTTG GTGCTGTTCC TTTCGTTGGC AGTGGTAAGG CCAGAGTGTC ACCAGAGAGC 
TCAGATATTT TTGCCCAAGC CCCTTTTAGG CAGGCTAGCC AGGAGAACGC TGCGGAAGAG 
ACTGACATAT TCATCAAAGC TCCTTTCAAC AGAAACCTCT CCAGGTCCAG CAGGAGTGGC 
GATGGACCCA GTGGCCAGAC GCCACCCGTT TCTCCTGACA GCGTGGATGT ATTCGGATTC 
TCGCCTTTCC AACCTAGCCA CGTAATGAAC AGGGATTCTC GGAATCAGGA GGACATCTTT 
GGCCAAGCAC CTTTCGACGA AGCAGACAGT CCCCAGCAAC AGAGGCAGAA GCAGTGCAGT 
CTTCAGAAAC TCTCCTCGCG GCAAAGGCGC ACCAAGCAAA AGGCCAGTGG CGGGAATGGG 
AAGCGACACC ACGGCACGCC CACTGGTGGC AGGAAGAGCA ACAAACCCAG TTTCCGTACC 
CCCGAACGGG TGCGAAGGCA CAAGAAAGTG GGCAGACGGG ACTCGCAGAG CAGCAATGAG 
TTCCTCAGCA CGTCTGACTC CAAAGAGAAC ATCAGTATTT CCCTGGGCGA GGTGATGGAA 
AAAGAAGCTG CGTTGCCTTC GGAGGAGGTC TTGTTGGATC CGTTTGGAGC CAAGCCTTTT 
CATCCACAAG ATGGCAGTCG ACATGGCCAG CACCAGAGCT TGGGAGACAA CAAAAGTGAG 
ATCAATTCGA CCAACGGTAG GCCGAGAACC AGTTCCCTAC ATGGCGTGTT TGAGGAAAAT 
AAGATAGATG ACTTTGGAGC TGTACCTTTC ACAGAACTAG TGGTCGAAAG CGGAGCTCCG 
CAAACCCCAC AAAAGGATCT CGACCCTTTC GGAGCTGCAC CTTTCCCCTC AAAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016108635.1
CDS	894..4193
Translation	MKKFSRMPKSESGSLGGGSGSGTGSSNYIGKVFAVGRYQVTVEELIAEGGFSMVFLARTHSGVRCALKRMYVNNVHDLNIFKREITIMKELSGHKNIVRYLDSTTNAVGDSVWEVLILMEYCKAGQVVKQMNQRLHIGFTEAEVLNIFCDACEAVARLHQCKTSIIHRDLKVENILLNDQGNYVLCDFGSATHKVLLPHKDGIAVVEDEIKKYTTLSYRAPEMINLYQGKAITTKADIWALGCLLYKLCFFTLPFGESQVAICDGTFAVPDSSKFSSRLHGLVRYMLEPDQEKRPDIYQVSYFAFRLAGKECPVPNLFNSPLPTSLPEPLTASDIAAKKSLTKARITDSIGPTETSIAPRQRPKAANSNVLPLSSSVTPVKMTGSSGTDSNGQKASGNGQQSAAQMPSSSQQNRVLQQLQPGDLRLQQLHHQQQQQQQQHNIHHQQQVTAQQLQYLQQYHQAMQQSLQMQQQHMLQQQMMMQQPIYQSQQQQQAHYTALMHQYQQAFVQQQQQQCAPVQQVPPLEFQIPLGSYNPAGPSPVDTPFANIRNPVSTMDVISPPPQTVNNPPDMSGWNPFGEDNFSKLTEEELLDREFDLLRAKKPVERSRSMEASSIDRQQPVLQPSLPEDPFGSVPFLANAGAGVLTAEHPVEQLSVAVTVPSTQDTTLQSVKEHKPAKKSSSTSSLPQKAGEESDSDFESDPPSPKSSEDEEAEEEEGLNSEREDTEPENLGQRPLLMDSEEEEDKHSSDSDCDSRKAKLGLSKEKAALFPPRTVNAESGPSVITPPNSPSVAMPVASIVDVFGAVPFVGSGKARVSPESSDIFAQAPFRQASQENAAEETDIFIKAPFNRNLSRSSRSGDGPSGQTPPVSPDSVDVFGFSPFQPSHVMNRDSRNQEDIFGQAPFDEADSPQQQRQKQCSLQKLSSRQRRTKQKASGGNGKRHHGTPTGGRKSNKPSFRTPERVRRHKKVGRRDSQSSNEFLSTSDSKENISISLGEVMEKEAALPSEEVLLDPFGAKPFHPQDGSRHGQHQSLGDNKSEINSTNGRPRTSSLHGVFEENKIDDFGAVPFTELVVESGAPQTPQKDLDPFGAAPFPSKQ

Target ORF information:

RefSeq Version	XM_016253149.1
Organism	Sinocyclocheilus grahami
Definition	Sinocyclocheilus grahami BMP2 inducible kinase (bmp2k), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016253149.1

ORF Insert Sequence:

ATGAAGAAGT TTTCGCGTAT GCCGAAATCA GAGAGCGGAA GTCTCGGGGG TGGGTCTGGA 
TCTGGTACTG GAAGCAGCAA CTATATCGGG AAGGTGTTTG CTGTCGGTCG ATACCAAGTG 
ACGGTGGAAG AGCTGATCGC TGAAGGAGGT TTCTCCATGG TGTTTCTGGC CCGCACACAC 
AGTGGAGTAC GATGTGCCCT GAAAAGGATG TATGTGAACA ATGTCCACGA CCTGAACATC 
TTCAAGCGGG AGATCACAAT CATGAAAGAG TTGTCGGGCC ACAAGAACAT TGTGCGGTAT 
CTGGACTCAA CCACCAATGC AGTTGGAGAC AGTGTATGGG AGGTTCTGAT TCTGATGGAG 
TATTGCAAAG CTGGTCAGGT GGTGAAGCAG ATGAATCAAC GGCTGCATAT AGGATTCACA 
GAAGCGGAGG TGCTCAACAT ATTCTGTGAC GCATGTGAAG CTGTTGCTAG ACTCCACCAG 
TGTAAAACTT CAATCATTCA CAGAGACCTA AAGGTAGAAA ACATCCTCCT AAATGACCAG 
GGAAACTACG TCTTGTGTGA CTTTGGAAGT GCCACACATA AAGTTCTGCT TCCACATAAA 
GATGGAATAG CAGTGGTGGA GGATGAGATC AAGAAGTACA CGACTCTGTC ATACCGTGCC 
CCAGAGATGA TTAACTTGTA CCAAGGGAAA GCCATCACCA CAAAGGCTGA TATCTGGGCA 
CTTGGATGCT TGTTGTACAA GCTGTGTTTC TTCACACTTC CATTTGGGGA GAGTCAAGTT 
GCCATATGTG ACGGAACCTT TGCTGTTCCG GACAGTTCAA AATTCTCCTC CAGGTTGCAC 
GGTTTAGTCA GATACATGTT GGAGCCTGAT CAAGAAAAGA GACCTGACAT CTATCAAGTT 
TCTTACTTTG CCTTTCGGTT AGCTGGAAAG GAGTGTCCAG TGCCAAATCT CTTTAACTCT 
CCCCTTCCCA CCTCGTTGCC AGAGCCCCTT ACTGCTAGTG ATATTGCTGC TAAAAAGAGC 
CTGACAAAAG CCAGAATAAC AGATTCAATT GGACCAACAG AAACATCAAT CGCACCCAGG 
CAAAGACCCA AAGCTGCAAA TAGTAATGTT TTACCTTTGT CCAGCTCTGT AACTCCTGTG 
AAAATGACTG GATCCTCGGG TACGGATAGC AACGGCCAGA AAGCTTCTGG AAACGGGCAG 
CAGTCAGCTG CACAGATGCC AAGCAGCAGT CAACAGAACC GGGTGTTACA GCAGCTGCAG 
CCTGGAGACC TTCGACTGCA ACAGCTACAT CATCAACAAC AACAACAGCA GCAGCAGCAC 
AATATACACC ATCAACAGCA AGTCACTGCA CAGCAGCTAC AGTACCTGCA ACAGTATCAT 
CAGGCCATGC AACAGAGCCT TCAGATGCAG CAACAGCATA TGCTTCAGCA GCAGATGATG 
ATGCAGCAGC CCATATATCA ATCCCAGCAG CAACAGCAGG CTCACTACAC TGCCTTGATG 
CATCAGTACC AGCAGGCCTT TGTTCAGCAG CAGCAGCAGC AATGTGCTCC AGTCCAACAG 
GTGCCCCCTC TGGAGTTCCA GATCCCACTG GGCTCCTATA ACCCTGCCGG ACCTTCTCCA 
GTAGACACCC CCTTTGCTAA CATCAGGAAC CCAGTGTCCA CTATGGATGT TATCAGCCCT 
CCTCCCCAGA CTGTCAATAA CCCTCCTGAT ATGTCTGGTT GGAACCCATT TGGAGAAGAC 
AATTTCTCCA AACTCACAGA AGAGGAGCTT CTCGATCGAG AGTTCGACCT TCTAAGAGCA 
AAAAAGCCGG TAGAGCGATC TCGCAGCATG GAGGCCAGCA GCATAGACAG ACAGCAGCCG 
GTACTGCAGC CCTCACTCCC TGAGGATCCA TTTGGCTCTG TGCCGTTCCT GGCCAACGCA 
GGAGCTGGTG TGCTGACAGC AGAGCATCCA GTTGAGCAGC TCAGTGTTGC TGTGACTGTT 
CCAAGCACGC AAGACACAAC CCTGCAATCA GTCAAAGAAC ACAAACCTGC AAAGAAAAGT 
AGCTCCACTA GTTCACTGCC TCAGAAAGCA GGCGAGGAAT CAGACAGTGA TTTTGAGTCG 
GACCCACCCT CTCCCAAAAG CAGTGAAGAC GAAGAGGCCG AAGAGGAGGA AGGTCTCAAC 
AGCGAACGTG AAGACACCGA ACCTGAGAAC CTGGGTCAGA GGCCGTTACT CATGGACTCT 
GAGGAAGAGG AGGACAAACA CAGCTCAGAC TCAGATTGTG ACTCTAGGAA AGCAAAACTT 
GGGTTGTCGA AGGAAAAGGC TGCACTTTTT CCTCCTAGAA CAGTGAACGC TGAATCTGGA 
CCCAGTGTGA TCACTCCTCC CAATTCGCCA AGTGTAGCCA TGCCTGTCGC GAGCATTGTG 
GATGTGTTTG GTGCTGTTCC TTTCGTTGGC AGTGGTAAGG CCAGAGTGTC ACCAGAGAGC 
TCAGATATTT TTGCCCAAGC CCCTTTTAGG CAGGCTAGCC AGGAGAACGC TGCGGAAGAG 
ACTGACATAT TCATCAAAGC TCCTTTCAAC AGAAACCTCT CCAGGTCCAG CAGGAGTGGC 
GATGGACCCA GTGGCCAGAC GCCACCCGTT TCTCCTGACA GCGTGGATGT ATTCGGATTC 
TCGCCTTTCC AACCTAGCCA CGTAATGAAC AGGGATTCTC GGAATCAGGA GGACATCTTT 
GGCCAAGCAC CTTTCGACGA AGCAGACAGT CCCCAGCAAC AGAGGCAGAA GCAGTGCAGT 
CTTCAGAAAC TCTCCTCGCG GCAAAGGCGC ACCAAGCAAA AGGCCAGTGG CGGGAATGGG 
AAGCGACACC ACGGCACGCC CACTGGTGGC AGGAAGAGCA ACAAACCCAG TTTCCGTACC 
CCCGAACGGG TGCGAAGGCA CAAGAAAGTG GGCAGACGGG ACTCGCAGAG CAGCAATGAG 
TTCCTCAGCA CGTCTGACTC CAAAGAGAAC ATCAGTATTT CCCTGGGCGA GGTGATGGAA 
AAAGAAGCTG CGTTGCCTTC GGAGGAGGTC TTGTTGGATC CGTTTGGAGC CAAGCCTTTT 
CATCCACAAG ATGGCAGTCG ACATGGCCAG CACCAGAGCT TGGGAGACAA CAAAAGTGAG 
ATCAATTCGA CCAACGGTAG GCCGAGAACC AGTTCCCTAC ATGGCGTGTT TGAGGAAAAT 
AAGATAGATG ACTTTGGAGC TGTACCTTTC ACAGAACTAG TGGTCGAAAG CGGAGCTCCG 
CAAACCCCAC AAAAGGATCT CGACCCTTTC GGAGCTGCAC CTTTCCCCTC AAAGCAGTGA