LOC107653898 cDNA ORF clone, Sinocyclocheilus anshuiensis

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC107653898
Entrez Gene ID	107653898
Full Name	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like
Gene Type	protein-coding
Organism	Sinocyclocheilus anshuiensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_016440764.1	XP_016296250.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like
XM_016440763.1	XP_016296249.1	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like

Pathways from BioSystems
Gene Ontology

The following LOC107653898 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC107653898 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi73042	XM_016440764.1 Latest version!	Sinocyclocheilus anshuiensis phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like (LOC107653898), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OSi73042	XM_016440763.1 Latest version!	Sinocyclocheilus anshuiensis phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like (LOC107653898), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC107653898 ( XM_016440764.1 ) cDNA ORF clone, Sinocyclocheilus anshuiensis -> XP_016296250.1 Sinocyclocheilus anshuiensis phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like (LOC107653898), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSi73042
Clone ID Related Accession (Same CDS sequence)	XM_016440764.1 , XM_016440763.1
Accession Version	XM_016440764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus anshuiensis
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015548216.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus anshuiensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCTC GGCCGTCCTC AGGAGAGTTA TGGGGGCTTC ACCTGATGCC TCCTCGGATC 
CTGGTGGACT GCTGTCTGCC GAACGGGATC CTAGTGAGTC TGGAGTGTCT GAGAGAAGCT 
CCTCTGACCA GCATCAAGCA GCAGCTCTTC AGCGAGGCAC GCAAATACCC GCTCTACCAC 
CTGCTGCAGG AGGAATCCTG CTACATCTTT GTGGGAGTGA CGCAGGAGGC CGAGCGCGAG 
GAGTTCTACG ACGAGACGCG GCGTCTGTGT GACCTGCGGC TCTTTCACCC CATCCTCAAA 
GTCATCGAGC CGCTGGGGAA CAGAGAGGAG AAGATCCTCA ACCGGGAGAT CGGTTTCGCT 
ATAGGGATGC CCATCTGTGA GTTCGAGCTG GTGAAGGATC CGGAGGTGCA GGACTTCCGC 
AGGAACATTC TGAGCGTGTG CAGGGAGGCG GTGGAGATGC GCGAGGGAGG AGGCGCACAC 
ACACAGGCGC TCTACGTCTA TCCTCCCAAC GTGGAGTCCT CCGCCGAGCT GCCGCAGCAC 
ATCTACAGCA AACTCGACAA AGGCCGTCTG ATCGTCACCA TCTGGGTGAT CGTGTCCCCG 
TCTAACTCCA AGCAGAAGTA CACTCTGAAG ATCACACACG ACTCTCTGCC GGAGCAGCTG 
ATCGCTGAAG CCATCCGCAA GAAGACGCGC AGCATGCATC TGTCAGCGCA GCAGCTGCGG 
CTCTGTGTGC AGGAGTACCA GGGCCAGTAC ATGCTGAAAG TGTGCGGCTG CGACGAGTAC 
CTCCTGGAGA AACACCCCCT CAGCCAGTAC AAGTACATCA GGAGCTGCAT CACGCTGGGC 
CGTCTGCCTC ACCTCATGCT GGTCAGTAAG GACAGTCTGT ACAGTCAGCT GCCCTCCAGT 
GGCTTCATGG CTCCGTCGTA CAGCCGGCGC ACACCTCAGC CCAGCCCCTG CCCCGGCGGA 
GGAGACCCCG GCTCACCACG CTCCCTCTGG GCCTTCAACA CACACCTGAG AGTCCGGCTG 
CTGTGTGCCA CATACGTCAA CGTCAACATT AGAGACATCG ACAAGATCTA CGTCAGGACA 
GGAATATATC ACGGAGGAGA ACCGCTGTGT GACAACGTCA ATACACAGAG AGTGCCCTGC 
TCCAACCCGA GGTGGAACGA GTGGCTGTCG TATGACATCT ACCTGACCGA TGTTCCTCGC 
TCTGCTCGTC TCTGTCTGTC CATCTGCTCC GTCAAGGGCC GCAAGGGAGC CAAAGAGGAA 
CACTGTCCTC TGGCCTGGGG AAACATCAAC CTCTTCGACT ATAAAGACAC GCTGGTGAGC 
GGGAAAGTGG CTCTGGGTTT GTGGCCGGTT CCTCACGGTC TGGAGGATCT GCTCAACCCC 
ATCGGAGTGT CCGGATCCAA CCCAAACAAG GAGACGCCGT GTGTGGAGCT GGAGTTCCCC 
TGGTTTAACC AGACTGTGGT GTTTCCTGAT GAGCTGCAGA TAGAAGAACA CGCCAACTGG 
AGCATCTCCC GTGAGCTGGG ATACAGCTAC TGTCTGGGGC TGAGCAGTCG TCTGGTGTGT 
GACAGCTCCA TATCGCAGGC GGACGCAGAG CAACTGCGCA CACTGTGCAG TCGAGACCCG 
CTGTATGAGC TCTCCGAACA AGAGAAAGAC TTCCTGTGGA GACATCGGCA TTACTGTGTG 
AATATTCCAG AAAGTCTTCC CAAACTCCTG CTGTCCGTCA AGTGGAACTC ACGGGACGAG 
GTGTCACAGA TCTACTGTCT GCTGAAGGAC TGGCCACTCA TGCAGCCTGA ATCCGCTCTG 
GAGCTGCTGG ACTGTAACTT CCCCGATCCC ATGGTGCGAG AGTTTGCCCT GCGCTGCCTG 
ATCCAGGGCC TGACGGACGA CAAGCTCAGC CAGTACCTGC TGCAGCTTGT GCAGGTACTG 
AAATATGAGA TGTATCTGGA TAACCCGTTA GCTCGGTTCC TGATCAAGAA AGCGCTGACC 
AATCAGAGGA TCGGACATTT CTTCTTCTGG CATCTGAAGT CTGAGATGCA CAATAAAACG 
GTGTCTCGGC GCTTCGGGCT GCTGCTGGAG GCGTTCTGTC GGGCGTGTGG GATGTACCTG 
AAACACCTGA ACAGACAGGT GGAGGCCATG GACAAGCTGG TGAACCTCAC AGACACACTC 
AAACAGGAGA AGAAAGACGA GACGCAGAAG ACTCAGATGA AGTTCCTGGT GGAGCACATG 
TCCCGCCCAG ATTACATGGA GGCGCTGCAG GGGTTTGTTT CTCCTCTGAA CCCCGTACAC 
CAGCTGGGCA ATCTCAGGTT GGAGGAGTGC CGTATCATGT CTTCAGCCAA GCGGCCGCTG 
TGGTTGAACT GGGAGAATCC GGATATCATG AGCGAGCTGC TCTTCACCAA TAATGAGATC 
ATCTTTAAAA ATGGAGATGA TCTACGGCAA GACATGCTGA CGCTGCAGAT TATTAAAATC 
ATGGAGAATA TCTGGCAGAA TCAGGGGCTG GACCTGCGAA TGCTGCCGTA TGGCTGTCTG 
TCCATCGGCG ACTGCGTGGG TCTCATTGAG GTGGTGAAGA ACTCGCACAC CATCATGCAG 
ATTCAGTGTA AAGGAGGCCT GAAGGGGGCG CTGCAGTTCA ACAGCAACAC ACTGCACCAC 
TGGATCAGAG ACAAGAACAA GGGAGAGGCG TACGACAGAG CCATCGATTT ATTCACACGC 
TCGTGTGCCG GATACTGTGT GGCCACGTTC ATTCTGGGCA TCGGCGACAG ACACAACAGC 
AACATCATGG TGAAGGAGAA CGGACAGCTG TTCCACATCG ACTTCGGCCA CTTTCTGGAT 
CATAAAAAGA AGAAATTTGG ATACAAGCGA GAGCGAGTTC CTTTCGTTCT CACGCAAGAC 
TTCCTGATCG TCATCAGTAA GGGTGTGCAG GAGTGCACCA AGACCAAAGA GTTCGAGAGG 
TTTCAGGAGA TGTGTTATAA AGCATATCTG GCCATCCGTC AGCACGCCAG CCTCTTCATC 
AACCTCTTCT CTCTGCTGCT GGGCTGTGGG ATGCCTGAGC TGCAGAGCTT CGACGACATC 
GCCTACCTGC GCAAGACACT GGCGCTGGAG AAGAACCAGC AGGAGGCTCT GGAGTACTTC 
ACCAAACAGA TGAACGACGC TCACCACGGA GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCA GACACATGCC CAATGAGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016296250.1
CDS	81..3293
Translation	MAPRPSSGELWGLHLMPPRILVDCCLPNGILVSLECLREAPLTSIKQQLFSEARKYPLYHLLQEESCYIFVGVTQEAEREEFYDETRRLCDLRLFHPILKVIEPLGNREEKILNREIGFAIGMPICEFELVKDPEVQDFRRNILSVCREAVEMREGGGAHTQALYVYPPNVESSAELPQHIYSKLDKGRLIVTIWVIVSPSNSKQKYTLKITHDSLPEQLIAEAIRKKTRSMHLSAQQLRLCVQEYQGQYMLKVCGCDEYLLEKHPLSQYKYIRSCITLGRLPHLMLVSKDSLYSQLPSSGFMAPSYSRRTPQPSPCPGGGDPGSPRSLWAFNTHLRVRLLCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLSYDIYLTDVPRSARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYKDTLVSGKVALGLWPVPHGLEDLLNPIGVSGSNPNKETPCVELEFPWFNQTVVFPDELQIEEHANWSISRELGYSYCLGLSSRLVCDSSISQADAEQLRTLCSRDPLYELSEQEKDFLWRHRHYCVNIPESLPKLLLSVKWNSRDEVSQIYCLLKDWPLMQPESALELLDCNFPDPMVREFALRCLIQGLTDDKLSQYLLQLVQVLKYEMYLDNPLARFLIKKALTNQRIGHFFFWHLKSEMHNKTVSRRFGLLLEAFCRACGMYLKHLNRQVEAMDKLVNLTDTLKQEKKDETQKTQMKFLVEHMSRPDYMEALQGFVSPLNPVHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFTNNEIIFKNGDDLRQDMLTLQIIKIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVKNSHTIMQIQCKGGLKGALQFNSNTLHHWIRDKNKGEAYDRAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKENGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGVQECTKTKEFERFQEMCYKAYLAIRQHASLFINLFSLLLGCGMPELQSFDDIAYLRKTLALEKNQQEALEYFTKQMNDAHHGGWTTKMDWIFHTIRHMPNEH

Target ORF information:

RefSeq Version	XM_016440764.1
Organism	Sinocyclocheilus anshuiensis
Definition	Sinocyclocheilus anshuiensis phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like (LOC107653898), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016440764.1

ORF Insert Sequence:

ATGGCTCCTC GGCCGTCCTC AGGAGAGTTA TGGGGGCTTC ACCTGATGCC TCCTCGGATC 
CTGGTGGACT GCTGTCTGCC GAACGGGATC CTAGTGAGTC TGGAGTGTCT GAGAGAAGCT 
CCTCTGACCA GCATCAAGCA GCAGCTCTTC AGCGAGGCAC GCAAATACCC GCTCTACCAC 
CTGCTGCAGG AGGAATCCTG CTACATCTTT GTGGGAGTGA CGCAGGAGGC CGAGCGCGAG 
GAGTTCTACG ACGAGACGCG GCGTCTGTGT GACCTGCGGC TCTTTCACCC CATCCTCAAA 
GTCATCGAGC CGCTGGGGAA CAGAGAGGAG AAGATCCTCA ACCGGGAGAT CGGTTTCGCT 
ATAGGGATGC CCATCTGTGA GTTCGAGCTG GTGAAGGATC CGGAGGTGCA GGACTTCCGC 
AGGAACATTC TGAGCGTGTG CAGGGAGGCG GTGGAGATGC GCGAGGGAGG AGGCGCACAC 
ACACAGGCGC TCTACGTCTA TCCTCCCAAC GTGGAGTCCT CCGCCGAGCT GCCGCAGCAC 
ATCTACAGCA AACTCGACAA AGGCCGTCTG ATCGTCACCA TCTGGGTGAT CGTGTCCCCG 
TCTAACTCCA AGCAGAAGTA CACTCTGAAG ATCACACACG ACTCTCTGCC GGAGCAGCTG 
ATCGCTGAAG CCATCCGCAA GAAGACGCGC AGCATGCATC TGTCAGCGCA GCAGCTGCGG 
CTCTGTGTGC AGGAGTACCA GGGCCAGTAC ATGCTGAAAG TGTGCGGCTG CGACGAGTAC 
CTCCTGGAGA AACACCCCCT CAGCCAGTAC AAGTACATCA GGAGCTGCAT CACGCTGGGC 
CGTCTGCCTC ACCTCATGCT GGTCAGTAAG GACAGTCTGT ACAGTCAGCT GCCCTCCAGT 
GGCTTCATGG CTCCGTCGTA CAGCCGGCGC ACACCTCAGC CCAGCCCCTG CCCCGGCGGA 
GGAGACCCCG GCTCACCACG CTCCCTCTGG GCCTTCAACA CACACCTGAG AGTCCGGCTG 
CTGTGTGCCA CATACGTCAA CGTCAACATT AGAGACATCG ACAAGATCTA CGTCAGGACA 
GGAATATATC ACGGAGGAGA ACCGCTGTGT GACAACGTCA ATACACAGAG AGTGCCCTGC 
TCCAACCCGA GGTGGAACGA GTGGCTGTCG TATGACATCT ACCTGACCGA TGTTCCTCGC 
TCTGCTCGTC TCTGTCTGTC CATCTGCTCC GTCAAGGGCC GCAAGGGAGC CAAAGAGGAA 
CACTGTCCTC TGGCCTGGGG AAACATCAAC CTCTTCGACT ATAAAGACAC GCTGGTGAGC 
GGGAAAGTGG CTCTGGGTTT GTGGCCGGTT CCTCACGGTC TGGAGGATCT GCTCAACCCC 
ATCGGAGTGT CCGGATCCAA CCCAAACAAG GAGACGCCGT GTGTGGAGCT GGAGTTCCCC 
TGGTTTAACC AGACTGTGGT GTTTCCTGAT GAGCTGCAGA TAGAAGAACA CGCCAACTGG 
AGCATCTCCC GTGAGCTGGG ATACAGCTAC TGTCTGGGGC TGAGCAGTCG TCTGGTGTGT 
GACAGCTCCA TATCGCAGGC GGACGCAGAG CAACTGCGCA CACTGTGCAG TCGAGACCCG 
CTGTATGAGC TCTCCGAACA AGAGAAAGAC TTCCTGTGGA GACATCGGCA TTACTGTGTG 
AATATTCCAG AAAGTCTTCC CAAACTCCTG CTGTCCGTCA AGTGGAACTC ACGGGACGAG 
GTGTCACAGA TCTACTGTCT GCTGAAGGAC TGGCCACTCA TGCAGCCTGA ATCCGCTCTG 
GAGCTGCTGG ACTGTAACTT CCCCGATCCC ATGGTGCGAG AGTTTGCCCT GCGCTGCCTG 
ATCCAGGGCC TGACGGACGA CAAGCTCAGC CAGTACCTGC TGCAGCTTGT GCAGGTACTG 
AAATATGAGA TGTATCTGGA TAACCCGTTA GCTCGGTTCC TGATCAAGAA AGCGCTGACC 
AATCAGAGGA TCGGACATTT CTTCTTCTGG CATCTGAAGT CTGAGATGCA CAATAAAACG 
GTGTCTCGGC GCTTCGGGCT GCTGCTGGAG GCGTTCTGTC GGGCGTGTGG GATGTACCTG 
AAACACCTGA ACAGACAGGT GGAGGCCATG GACAAGCTGG TGAACCTCAC AGACACACTC 
AAACAGGAGA AGAAAGACGA GACGCAGAAG ACTCAGATGA AGTTCCTGGT GGAGCACATG 
TCCCGCCCAG ATTACATGGA GGCGCTGCAG GGGTTTGTTT CTCCTCTGAA CCCCGTACAC 
CAGCTGGGCA ATCTCAGGTT GGAGGAGTGC CGTATCATGT CTTCAGCCAA GCGGCCGCTG 
TGGTTGAACT GGGAGAATCC GGATATCATG AGCGAGCTGC TCTTCACCAA TAATGAGATC 
ATCTTTAAAA ATGGAGATGA TCTACGGCAA GACATGCTGA CGCTGCAGAT TATTAAAATC 
ATGGAGAATA TCTGGCAGAA TCAGGGGCTG GACCTGCGAA TGCTGCCGTA TGGCTGTCTG 
TCCATCGGCG ACTGCGTGGG TCTCATTGAG GTGGTGAAGA ACTCGCACAC CATCATGCAG 
ATTCAGTGTA AAGGAGGCCT GAAGGGGGCG CTGCAGTTCA ACAGCAACAC ACTGCACCAC 
TGGATCAGAG ACAAGAACAA GGGAGAGGCG TACGACAGAG CCATCGATTT ATTCACACGC 
TCGTGTGCCG GATACTGTGT GGCCACGTTC ATTCTGGGCA TCGGCGACAG ACACAACAGC 
AACATCATGG TGAAGGAGAA CGGACAGCTG TTCCACATCG ACTTCGGCCA CTTTCTGGAT 
CATAAAAAGA AGAAATTTGG ATACAAGCGA GAGCGAGTTC CTTTCGTTCT CACGCAAGAC 
TTCCTGATCG TCATCAGTAA GGGTGTGCAG GAGTGCACCA AGACCAAAGA GTTCGAGAGG 
TTTCAGGAGA TGTGTTATAA AGCATATCTG GCCATCCGTC AGCACGCCAG CCTCTTCATC 
AACCTCTTCT CTCTGCTGCT GGGCTGTGGG ATGCCTGAGC TGCAGAGCTT CGACGACATC 
GCCTACCTGC GCAAGACACT GGCGCTGGAG AAGAACCAGC AGGAGGCTCT GGAGTACTTC 
ACCAAACAGA TGAACGACGC TCACCACGGA GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCA GACACATGCC CAATGAGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107653898 ( XM_016440763.1 ) cDNA ORF clone, Sinocyclocheilus anshuiensis -> XP_016296249.1 Sinocyclocheilus anshuiensis phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like (LOC107653898), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSi73042
Clone ID Related Accession (Same CDS sequence)	XM_016440764.1 , XM_016440763.1
Accession Version	XM_016440763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-05-02
Organism	Sinocyclocheilus anshuiensis
Product	phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015548216.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Sinocyclocheilus anshuiensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCTC GGCCGTCCTC AGGAGAGTTA TGGGGGCTTC ACCTGATGCC TCCTCGGATC 
CTGGTGGACT GCTGTCTGCC GAACGGGATC CTAGTGAGTC TGGAGTGTCT GAGAGAAGCT 
CCTCTGACCA GCATCAAGCA GCAGCTCTTC AGCGAGGCAC GCAAATACCC GCTCTACCAC 
CTGCTGCAGG AGGAATCCTG CTACATCTTT GTGGGAGTGA CGCAGGAGGC CGAGCGCGAG 
GAGTTCTACG ACGAGACGCG GCGTCTGTGT GACCTGCGGC TCTTTCACCC CATCCTCAAA 
GTCATCGAGC CGCTGGGGAA CAGAGAGGAG AAGATCCTCA ACCGGGAGAT CGGTTTCGCT 
ATAGGGATGC CCATCTGTGA GTTCGAGCTG GTGAAGGATC CGGAGGTGCA GGACTTCCGC 
AGGAACATTC TGAGCGTGTG CAGGGAGGCG GTGGAGATGC GCGAGGGAGG AGGCGCACAC 
ACACAGGCGC TCTACGTCTA TCCTCCCAAC GTGGAGTCCT CCGCCGAGCT GCCGCAGCAC 
ATCTACAGCA AACTCGACAA AGGCCGTCTG ATCGTCACCA TCTGGGTGAT CGTGTCCCCG 
TCTAACTCCA AGCAGAAGTA CACTCTGAAG ATCACACACG ACTCTCTGCC GGAGCAGCTG 
ATCGCTGAAG CCATCCGCAA GAAGACGCGC AGCATGCATC TGTCAGCGCA GCAGCTGCGG 
CTCTGTGTGC AGGAGTACCA GGGCCAGTAC ATGCTGAAAG TGTGCGGCTG CGACGAGTAC 
CTCCTGGAGA AACACCCCCT CAGCCAGTAC AAGTACATCA GGAGCTGCAT CACGCTGGGC 
CGTCTGCCTC ACCTCATGCT GGTCAGTAAG GACAGTCTGT ACAGTCAGCT GCCCTCCAGT 
GGCTTCATGG CTCCGTCGTA CAGCCGGCGC ACACCTCAGC CCAGCCCCTG CCCCGGCGGA 
GGAGACCCCG GCTCACCACG CTCCCTCTGG GCCTTCAACA CACACCTGAG AGTCCGGCTG 
CTGTGTGCCA CATACGTCAA CGTCAACATT AGAGACATCG ACAAGATCTA CGTCAGGACA 
GGAATATATC ACGGAGGAGA ACCGCTGTGT GACAACGTCA ATACACAGAG AGTGCCCTGC 
TCCAACCCGA GGTGGAACGA GTGGCTGTCG TATGACATCT ACCTGACCGA TGTTCCTCGC 
TCTGCTCGTC TCTGTCTGTC CATCTGCTCC GTCAAGGGCC GCAAGGGAGC CAAAGAGGAA 
CACTGTCCTC TGGCCTGGGG AAACATCAAC CTCTTCGACT ATAAAGACAC GCTGGTGAGC 
GGGAAAGTGG CTCTGGGTTT GTGGCCGGTT CCTCACGGTC TGGAGGATCT GCTCAACCCC 
ATCGGAGTGT CCGGATCCAA CCCAAACAAG GAGACGCCGT GTGTGGAGCT GGAGTTCCCC 
TGGTTTAACC AGACTGTGGT GTTTCCTGAT GAGCTGCAGA TAGAAGAACA CGCCAACTGG 
AGCATCTCCC GTGAGCTGGG ATACAGCTAC TGTCTGGGGC TGAGCAGTCG TCTGGTGTGT 
GACAGCTCCA TATCGCAGGC GGACGCAGAG CAACTGCGCA CACTGTGCAG TCGAGACCCG 
CTGTATGAGC TCTCCGAACA AGAGAAAGAC TTCCTGTGGA GACATCGGCA TTACTGTGTG 
AATATTCCAG AAAGTCTTCC CAAACTCCTG CTGTCCGTCA AGTGGAACTC ACGGGACGAG 
GTGTCACAGA TCTACTGTCT GCTGAAGGAC TGGCCACTCA TGCAGCCTGA ATCCGCTCTG 
GAGCTGCTGG ACTGTAACTT CCCCGATCCC ATGGTGCGAG AGTTTGCCCT GCGCTGCCTG 
ATCCAGGGCC TGACGGACGA CAAGCTCAGC CAGTACCTGC TGCAGCTTGT GCAGGTACTG 
AAATATGAGA TGTATCTGGA TAACCCGTTA GCTCGGTTCC TGATCAAGAA AGCGCTGACC 
AATCAGAGGA TCGGACATTT CTTCTTCTGG CATCTGAAGT CTGAGATGCA CAATAAAACG 
GTGTCTCGGC GCTTCGGGCT GCTGCTGGAG GCGTTCTGTC GGGCGTGTGG GATGTACCTG 
AAACACCTGA ACAGACAGGT GGAGGCCATG GACAAGCTGG TGAACCTCAC AGACACACTC 
AAACAGGAGA AGAAAGACGA GACGCAGAAG ACTCAGATGA AGTTCCTGGT GGAGCACATG 
TCCCGCCCAG ATTACATGGA GGCGCTGCAG GGGTTTGTTT CTCCTCTGAA CCCCGTACAC 
CAGCTGGGCA ATCTCAGGTT GGAGGAGTGC CGTATCATGT CTTCAGCCAA GCGGCCGCTG 
TGGTTGAACT GGGAGAATCC GGATATCATG AGCGAGCTGC TCTTCACCAA TAATGAGATC 
ATCTTTAAAA ATGGAGATGA TCTACGGCAA GACATGCTGA CGCTGCAGAT TATTAAAATC 
ATGGAGAATA TCTGGCAGAA TCAGGGGCTG GACCTGCGAA TGCTGCCGTA TGGCTGTCTG 
TCCATCGGCG ACTGCGTGGG TCTCATTGAG GTGGTGAAGA ACTCGCACAC CATCATGCAG 
ATTCAGTGTA AAGGAGGCCT GAAGGGGGCG CTGCAGTTCA ACAGCAACAC ACTGCACCAC 
TGGATCAGAG ACAAGAACAA GGGAGAGGCG TACGACAGAG CCATCGATTT ATTCACACGC 
TCGTGTGCCG GATACTGTGT GGCCACGTTC ATTCTGGGCA TCGGCGACAG ACACAACAGC 
AACATCATGG TGAAGGAGAA CGGACAGCTG TTCCACATCG ACTTCGGCCA CTTTCTGGAT 
CATAAAAAGA AGAAATTTGG ATACAAGCGA GAGCGAGTTC CTTTCGTTCT CACGCAAGAC 
TTCCTGATCG TCATCAGTAA GGGTGTGCAG GAGTGCACCA AGACCAAAGA GTTCGAGAGG 
TTTCAGGAGA TGTGTTATAA AGCATATCTG GCCATCCGTC AGCACGCCAG CCTCTTCATC 
AACCTCTTCT CTCTGCTGCT GGGCTGTGGG ATGCCTGAGC TGCAGAGCTT CGACGACATC 
GCCTACCTGC GCAAGACACT GGCGCTGGAG AAGAACCAGC AGGAGGCTCT GGAGTACTTC 
ACCAAACAGA TGAACGACGC TCACCACGGA GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCA GACACATGCC CAATGAGCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016296249.1
CDS	344..3556
Translation	MAPRPSSGELWGLHLMPPRILVDCCLPNGILVSLECLREAPLTSIKQQLFSEARKYPLYHLLQEESCYIFVGVTQEAEREEFYDETRRLCDLRLFHPILKVIEPLGNREEKILNREIGFAIGMPICEFELVKDPEVQDFRRNILSVCREAVEMREGGGAHTQALYVYPPNVESSAELPQHIYSKLDKGRLIVTIWVIVSPSNSKQKYTLKITHDSLPEQLIAEAIRKKTRSMHLSAQQLRLCVQEYQGQYMLKVCGCDEYLLEKHPLSQYKYIRSCITLGRLPHLMLVSKDSLYSQLPSSGFMAPSYSRRTPQPSPCPGGGDPGSPRSLWAFNTHLRVRLLCATYVNVNIRDIDKIYVRTGIYHGGEPLCDNVNTQRVPCSNPRWNEWLSYDIYLTDVPRSARLCLSICSVKGRKGAKEEHCPLAWGNINLFDYKDTLVSGKVALGLWPVPHGLEDLLNPIGVSGSNPNKETPCVELEFPWFNQTVVFPDELQIEEHANWSISRELGYSYCLGLSSRLVCDSSISQADAEQLRTLCSRDPLYELSEQEKDFLWRHRHYCVNIPESLPKLLLSVKWNSRDEVSQIYCLLKDWPLMQPESALELLDCNFPDPMVREFALRCLIQGLTDDKLSQYLLQLVQVLKYEMYLDNPLARFLIKKALTNQRIGHFFFWHLKSEMHNKTVSRRFGLLLEAFCRACGMYLKHLNRQVEAMDKLVNLTDTLKQEKKDETQKTQMKFLVEHMSRPDYMEALQGFVSPLNPVHQLGNLRLEECRIMSSAKRPLWLNWENPDIMSELLFTNNEIIFKNGDDLRQDMLTLQIIKIMENIWQNQGLDLRMLPYGCLSIGDCVGLIEVVKNSHTIMQIQCKGGLKGALQFNSNTLHHWIRDKNKGEAYDRAIDLFTRSCAGYCVATFILGIGDRHNSNIMVKENGQLFHIDFGHFLDHKKKKFGYKRERVPFVLTQDFLIVISKGVQECTKTKEFERFQEMCYKAYLAIRQHASLFINLFSLLLGCGMPELQSFDDIAYLRKTLALEKNQQEALEYFTKQMNDAHHGGWTTKMDWIFHTIRHMPNEH

Target ORF information:

RefSeq Version	XM_016440763.1
Organism	Sinocyclocheilus anshuiensis
Definition	Sinocyclocheilus anshuiensis phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform-like (LOC107653898), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016440763.1

ORF Insert Sequence:

ATGGCTCCTC GGCCGTCCTC AGGAGAGTTA TGGGGGCTTC ACCTGATGCC TCCTCGGATC 
CTGGTGGACT GCTGTCTGCC GAACGGGATC CTAGTGAGTC TGGAGTGTCT GAGAGAAGCT 
CCTCTGACCA GCATCAAGCA GCAGCTCTTC AGCGAGGCAC GCAAATACCC GCTCTACCAC 
CTGCTGCAGG AGGAATCCTG CTACATCTTT GTGGGAGTGA CGCAGGAGGC CGAGCGCGAG 
GAGTTCTACG ACGAGACGCG GCGTCTGTGT GACCTGCGGC TCTTTCACCC CATCCTCAAA 
GTCATCGAGC CGCTGGGGAA CAGAGAGGAG AAGATCCTCA ACCGGGAGAT CGGTTTCGCT 
ATAGGGATGC CCATCTGTGA GTTCGAGCTG GTGAAGGATC CGGAGGTGCA GGACTTCCGC 
AGGAACATTC TGAGCGTGTG CAGGGAGGCG GTGGAGATGC GCGAGGGAGG AGGCGCACAC 
ACACAGGCGC TCTACGTCTA TCCTCCCAAC GTGGAGTCCT CCGCCGAGCT GCCGCAGCAC 
ATCTACAGCA AACTCGACAA AGGCCGTCTG ATCGTCACCA TCTGGGTGAT CGTGTCCCCG 
TCTAACTCCA AGCAGAAGTA CACTCTGAAG ATCACACACG ACTCTCTGCC GGAGCAGCTG 
ATCGCTGAAG CCATCCGCAA GAAGACGCGC AGCATGCATC TGTCAGCGCA GCAGCTGCGG 
CTCTGTGTGC AGGAGTACCA GGGCCAGTAC ATGCTGAAAG TGTGCGGCTG CGACGAGTAC 
CTCCTGGAGA AACACCCCCT CAGCCAGTAC AAGTACATCA GGAGCTGCAT CACGCTGGGC 
CGTCTGCCTC ACCTCATGCT GGTCAGTAAG GACAGTCTGT ACAGTCAGCT GCCCTCCAGT 
GGCTTCATGG CTCCGTCGTA CAGCCGGCGC ACACCTCAGC CCAGCCCCTG CCCCGGCGGA 
GGAGACCCCG GCTCACCACG CTCCCTCTGG GCCTTCAACA CACACCTGAG AGTCCGGCTG 
CTGTGTGCCA CATACGTCAA CGTCAACATT AGAGACATCG ACAAGATCTA CGTCAGGACA 
GGAATATATC ACGGAGGAGA ACCGCTGTGT GACAACGTCA ATACACAGAG AGTGCCCTGC 
TCCAACCCGA GGTGGAACGA GTGGCTGTCG TATGACATCT ACCTGACCGA TGTTCCTCGC 
TCTGCTCGTC TCTGTCTGTC CATCTGCTCC GTCAAGGGCC GCAAGGGAGC CAAAGAGGAA 
CACTGTCCTC TGGCCTGGGG AAACATCAAC CTCTTCGACT ATAAAGACAC GCTGGTGAGC 
GGGAAAGTGG CTCTGGGTTT GTGGCCGGTT CCTCACGGTC TGGAGGATCT GCTCAACCCC 
ATCGGAGTGT CCGGATCCAA CCCAAACAAG GAGACGCCGT GTGTGGAGCT GGAGTTCCCC 
TGGTTTAACC AGACTGTGGT GTTTCCTGAT GAGCTGCAGA TAGAAGAACA CGCCAACTGG 
AGCATCTCCC GTGAGCTGGG ATACAGCTAC TGTCTGGGGC TGAGCAGTCG TCTGGTGTGT 
GACAGCTCCA TATCGCAGGC GGACGCAGAG CAACTGCGCA CACTGTGCAG TCGAGACCCG 
CTGTATGAGC TCTCCGAACA AGAGAAAGAC TTCCTGTGGA GACATCGGCA TTACTGTGTG 
AATATTCCAG AAAGTCTTCC CAAACTCCTG CTGTCCGTCA AGTGGAACTC ACGGGACGAG 
GTGTCACAGA TCTACTGTCT GCTGAAGGAC TGGCCACTCA TGCAGCCTGA ATCCGCTCTG 
GAGCTGCTGG ACTGTAACTT CCCCGATCCC ATGGTGCGAG AGTTTGCCCT GCGCTGCCTG 
ATCCAGGGCC TGACGGACGA CAAGCTCAGC CAGTACCTGC TGCAGCTTGT GCAGGTACTG 
AAATATGAGA TGTATCTGGA TAACCCGTTA GCTCGGTTCC TGATCAAGAA AGCGCTGACC 
AATCAGAGGA TCGGACATTT CTTCTTCTGG CATCTGAAGT CTGAGATGCA CAATAAAACG 
GTGTCTCGGC GCTTCGGGCT GCTGCTGGAG GCGTTCTGTC GGGCGTGTGG GATGTACCTG 
AAACACCTGA ACAGACAGGT GGAGGCCATG GACAAGCTGG TGAACCTCAC AGACACACTC 
AAACAGGAGA AGAAAGACGA GACGCAGAAG ACTCAGATGA AGTTCCTGGT GGAGCACATG 
TCCCGCCCAG ATTACATGGA GGCGCTGCAG GGGTTTGTTT CTCCTCTGAA CCCCGTACAC 
CAGCTGGGCA ATCTCAGGTT GGAGGAGTGC CGTATCATGT CTTCAGCCAA GCGGCCGCTG 
TGGTTGAACT GGGAGAATCC GGATATCATG AGCGAGCTGC TCTTCACCAA TAATGAGATC 
ATCTTTAAAA ATGGAGATGA TCTACGGCAA GACATGCTGA CGCTGCAGAT TATTAAAATC 
ATGGAGAATA TCTGGCAGAA TCAGGGGCTG GACCTGCGAA TGCTGCCGTA TGGCTGTCTG 
TCCATCGGCG ACTGCGTGGG TCTCATTGAG GTGGTGAAGA ACTCGCACAC CATCATGCAG 
ATTCAGTGTA AAGGAGGCCT GAAGGGGGCG CTGCAGTTCA ACAGCAACAC ACTGCACCAC 
TGGATCAGAG ACAAGAACAA GGGAGAGGCG TACGACAGAG CCATCGATTT ATTCACACGC 
TCGTGTGCCG GATACTGTGT GGCCACGTTC ATTCTGGGCA TCGGCGACAG ACACAACAGC 
AACATCATGG TGAAGGAGAA CGGACAGCTG TTCCACATCG ACTTCGGCCA CTTTCTGGAT 
CATAAAAAGA AGAAATTTGG ATACAAGCGA GAGCGAGTTC CTTTCGTTCT CACGCAAGAC 
TTCCTGATCG TCATCAGTAA GGGTGTGCAG GAGTGCACCA AGACCAAAGA GTTCGAGAGG 
TTTCAGGAGA TGTGTTATAA AGCATATCTG GCCATCCGTC AGCACGCCAG CCTCTTCATC 
AACCTCTTCT CTCTGCTGCT GGGCTGTGGG ATGCCTGAGC TGCAGAGCTT CGACGACATC 
GCCTACCTGC GCAAGACACT GGCGCTGGAG AAGAACCAGC AGGAGGCTCT GGAGTACTTC 
ACCAAACAGA TGAACGACGC TCACCACGGA GGATGGACCA CCAAGATGGA CTGGATCTTC 
CACACCATCA GACACATGCC CAATGAGCAC TGA