LOC111232478 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC111232478
Entrez Gene ID	111232478
Full Name	E3 ubiquitin-protein ligase RNF38-like
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022760026.1	XP_022615747.1	E3 ubiquitin-protein ligase RNF38-like

Pathways from BioSystems
Gene Ontology

The following LOC111232478 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC111232478 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe205643	XM_022760026.1 Latest version!	Seriola dumerili E3 ubiquitin-protein ligase RNF38-like (LOC111232478), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC111232478 ( XM_022760026.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022615747.1 Seriola dumerili E3 ubiquitin-protein ligase RNF38-like (LOC111232478), mRNA.

Price & Availability↑

CloneID	OSe205643
Clone ID Related Accession (Same CDS sequence)	XM_022760026.1
Accession Version	XM_022760026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	E3 ubiquitin-protein ligase RNF38-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174586.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCCC CGAGGACGCG GTCGCGGTCT CGGTCTGGCT TCTATCATTT CGGCATGAAT 
GGCAGCGTTG GAAGCAATGG TAGTGGTAAC ACGGTCGGTA ACGGGAGCTT GATGAACGCC 
AGCGGAGGAG GGGTGAGCTA CCCGCAGCAG AGCAACCCTG GCTGGGCGCC GCACCACGGT 
GGTCGTAGCT ACCCGGAGAG CCAGCAGCAA CACACCCAGG GGAGCTACCT GTCCGCAGGG 
GCGCAGCGCC ACTCTTCGCA CGGAGCTCAC AGACACCCCG GGGCCGGAGG AGTGCTACAT 
TTTCATCCGG ATAATGTGTG CAGCGAGGAT CGGGACAAAA TGGAAGACAG CCCAAGCCCC 
AAGAGGCAGC GTCTTTCTCA GCAGTCCATG TTGGATTTGA GTTCTGCCCC CCCCTCTACT 
CCTTCCTCTC CCATTCGCCC CTGGGAGCTG CCTCCCAGTC GCAGACCACA CCCCCACTAC 
ATGCCAGAGA GATGCCACAC ACCTGTTCGC AACCGACGCA GCCCTCCAAT GAGACGCCAG 
CGTGGCCGGA GAGACCGCCT GACCCGCCAC CACCACCATG CCCACAACAA CCACCACCAC 
TACAACAGCA ACAACAACAA CCACCACCAT CACCACCACC CCAACGCCCA CCATCATCAC 
CACCACCACC TGCATTCTCA CCACGGTCTG TCGTCAGGCC ACCAGGATGA AAACTACCGC 
CACCAGGCTC CCCCTCAGGG CTACCCACCC TACAGCCAGC AACCACCCCG AGGGCCTGGG 
CCTGGGCCGG GGCCCGGGCC TGAAGAGCGC CCAGGTTACC ATCCCCCAAA CCCTTCCCCG 
AGGCCCCTCC ACCAGTCACC GAACCTGTCG CCCAGGCTGC TGCACCCTGC GGCCCACCCA 
CAGCACCCAC ACCCTCACCC ATCCCAGCAA CAGAGCAGTG TGGTGCTTGA CCTCCATGAG 
CAGGGTTCAG CTCCAGTATC GTATCCTGTG TCTCCTCCGG GAGCGCCACC TGGCCTGCCG 
CCTCGCTCTG CCCCTCAGCA GATCCCAGCA TGCTCGGTGG TTTTCAGTGG ACAGCACTAT 
CCCGTCTGCA GCGTCCCTCC TCCCGTCATT CAGACCTGTT CTGTGCAGCA CTTACCGATG 
CCCTATCCCT TCCCATCACT GCTGTCCAGT GACCCGGCCT TCCTACTTCC CCCTCCTCAC 
CTCCCCCATC CCCCAACACA CCTTTCCCAC CACCCACCTC ACCTGGCCCA GCCTGGACAG 
TTTGGACCCT ATCCGACACA GCAGGCACGA TCGCCATTAC AAAGGATAGA GAATGATGTG 
GAGCTGCTTG GAGAGCACCT GTCTCTGGGT GCTGGGCTCC ACTATCCCCC CGCCACCCAC 
CCAGCCCTCA CCCCACACTC CACACAGCTT CACTTCCTTT CCCATGACCC CCTGCCACAG 
GAGTTCTTTG GAGTGTCCTA TCCAAACTTC ATTCCTCGGC GTCTCCCGGG GCGACGGTAC 
CGCTCACAAC AGCCACTGCC ACCTTCGCCT TACCACCCCA GCCTCCTGCC TTACTTCCTT 
TCGATGCTGC CAGTCCAGCC CACAGGTCCT GCGATCAGCC TAGAGCTGGA TGTAGACGAT 
GGAGAGGTGG AAAATTATGA GGCACTCCTG AACCTCGCTG AGCGTCTGGG AGAGGCCAAA 
CTCCGAGGAC TGACCAAAGG AGACATTGAA CAGCTTCCCT CCTATCGGTT CAATCCAAAT 
AACCATCAGT CTGAGCAAAC ACTGTGTGTG GTGTGTATGA GTGATTTTGA GTCCCGCCAA 
CTGCTGCGAG TCCTGCCCTG CAGCCACGAG TTCCACGGGA AATGTGTCGA CAAGTGGCTG 
AGGGCTAACA GGACGTGTCC CATTTGTCGG GCTGACGCCT CAGAGGTCCA AAGAGACTCA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022615747.1
CDS	1106..3031
Translation	MDPPRTRSRSRSGFYHFGMNGSVGSNGSGNTVGNGSLMNASGGGVSYPQQSNPGWAPHHGGRSYPESQQQHTQGSYLSAGAQRHSSHGAHRHPGAGGVLHFHPDNVCSEDRDKMEDSPSPKRQRLSQQSMLDLSSAPPSTPSSPIRPWELPPSRRPHPHYMPERCHTPVRNRRSPPMRRQRGRRDRLTRHHHHAHNNHHHYNSNNNNHHHHHHPNAHHHHHHHLHSHHGLSSGHQDENYRHQAPPQGYPPYSQQPPRGPGPGPGPGPEERPGYHPPNPSPRPLHQSPNLSPRLLHPAAHPQHPHPHPSQQQSSVVLDLHEQGSAPVSYPVSPPGAPPGLPPRSAPQQIPACSVVFSGQHYPVCSVPPPVIQTCSVQHLPMPYPFPSLLSSDPAFLLPPPHLPHPPTHLSHHPPHLAQPGQFGPYPTQQARSPLQRIENDVELLGEHLSLGAGLHYPPATHPALTPHSTQLHFLSHDPLPQEFFGVSYPNFIPRRLPGRRYRSQQPLPPSPYHPSLLPYFLSMLPVQPTGPAISLELDVDDGEVENYEALLNLAERLGEAKLRGLTKGDIEQLPSYRFNPNNHQSEQTLCVVCMSDFESRQLLRVLPCSHEFHGKCVDKWLRANRTCPICRADASEVQRDSE

Target ORF information:

RefSeq Version	XM_022760026.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili E3 ubiquitin-protein ligase RNF38-like (LOC111232478), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022760026.1

ORF Insert Sequence:

ATGGACCCCC CGAGGACGCG GTCGCGGTCT CGGTCTGGCT TCTATCATTT CGGCATGAAT 
GGCAGCGTTG GAAGCAATGG TAGTGGTAAC ACGGTCGGTA ACGGGAGCTT GATGAACGCC 
AGCGGAGGAG GGGTGAGCTA CCCGCAGCAG AGCAACCCTG GCTGGGCGCC GCACCACGGT 
GGTCGTAGCT ACCCGGAGAG CCAGCAGCAA CACACCCAGG GGAGCTACCT GTCCGCAGGG 
GCGCAGCGCC ACTCTTCGCA CGGAGCTCAC AGACACCCCG GGGCCGGAGG AGTGCTACAT 
TTTCATCCGG ATAATGTGTG CAGCGAGGAT CGGGACAAAA TGGAAGACAG CCCAAGCCCC 
AAGAGGCAGC GTCTTTCTCA GCAGTCCATG TTGGATTTGA GTTCTGCCCC CCCCTCTACT 
CCTTCCTCTC CCATTCGCCC CTGGGAGCTG CCTCCCAGTC GCAGACCACA CCCCCACTAC 
ATGCCAGAGA GATGCCACAC ACCTGTTCGC AACCGACGCA GCCCTCCAAT GAGACGCCAG 
CGTGGCCGGA GAGACCGCCT GACCCGCCAC CACCACCATG CCCACAACAA CCACCACCAC 
TACAACAGCA ACAACAACAA CCACCACCAT CACCACCACC CCAACGCCCA CCATCATCAC 
CACCACCACC TGCATTCTCA CCACGGTCTG TCGTCAGGCC ACCAGGATGA AAACTACCGC 
CACCAGGCTC CCCCTCAGGG CTACCCACCC TACAGCCAGC AACCACCCCG AGGGCCTGGG 
CCTGGGCCGG GGCCCGGGCC TGAAGAGCGC CCAGGTTACC ATCCCCCAAA CCCTTCCCCG 
AGGCCCCTCC ACCAGTCACC GAACCTGTCG CCCAGGCTGC TGCACCCTGC GGCCCACCCA 
CAGCACCCAC ACCCTCACCC ATCCCAGCAA CAGAGCAGTG TGGTGCTTGA CCTCCATGAG 
CAGGGTTCAG CTCCAGTATC GTATCCTGTG TCTCCTCCGG GAGCGCCACC TGGCCTGCCG 
CCTCGCTCTG CCCCTCAGCA GATCCCAGCA TGCTCGGTGG TTTTCAGTGG ACAGCACTAT 
CCCGTCTGCA GCGTCCCTCC TCCCGTCATT CAGACCTGTT CTGTGCAGCA CTTACCGATG 
CCCTATCCCT TCCCATCACT GCTGTCCAGT GACCCGGCCT TCCTACTTCC CCCTCCTCAC 
CTCCCCCATC CCCCAACACA CCTTTCCCAC CACCCACCTC ACCTGGCCCA GCCTGGACAG 
TTTGGACCCT ATCCGACACA GCAGGCACGA TCGCCATTAC AAAGGATAGA GAATGATGTG 
GAGCTGCTTG GAGAGCACCT GTCTCTGGGT GCTGGGCTCC ACTATCCCCC CGCCACCCAC 
CCAGCCCTCA CCCCACACTC CACACAGCTT CACTTCCTTT CCCATGACCC CCTGCCACAG 
GAGTTCTTTG GAGTGTCCTA TCCAAACTTC ATTCCTCGGC GTCTCCCGGG GCGACGGTAC 
CGCTCACAAC AGCCACTGCC ACCTTCGCCT TACCACCCCA GCCTCCTGCC TTACTTCCTT 
TCGATGCTGC CAGTCCAGCC CACAGGTCCT GCGATCAGCC TAGAGCTGGA TGTAGACGAT 
GGAGAGGTGG AAAATTATGA GGCACTCCTG AACCTCGCTG AGCGTCTGGG AGAGGCCAAA 
CTCCGAGGAC TGACCAAAGG AGACATTGAA CAGCTTCCCT CCTATCGGTT CAATCCAAAT 
AACCATCAGT CTGAGCAAAC ACTGTGTGTG GTGTGTATGA GTGATTTTGA GTCCCGCCAA 
CTGCTGCGAG TCCTGCCCTG CAGCCACGAG TTCCACGGGA AATGTGTCGA CAAGTGGCTG 
AGGGCTAACA GGACGTGTCC CATTTGTCGG GCTGACGCCT CAGAGGTCCA AAGAGACTCA 
GAGTGA