LOC111231860 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC111231860
Entrez Gene ID	111231860
Full Name	serine/threonine-protein kinase ULK1-like
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022759122.1	XP_022614843.1	serine/threonine-protein kinase ULK1-like isoform X1
XM_022759128.1	XP_022614849.1	serine/threonine-protein kinase ULK1-like isoform X2

Pathways from BioSystems
Gene Ontology

The following LOC111231860 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC111231860 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe208164	XM_022759122.1 Latest version!	Seriola dumerili serine/threonine-protein kinase ULK1-like (LOC111231860), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OSe208165	XM_022759128.1 Latest version!	Seriola dumerili serine/threonine-protein kinase ULK1-like (LOC111231860), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC111231860 ( XM_022759122.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022614843.1 Seriola dumerili serine/threonine-protein kinase ULK1-like (LOC111231860), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe208164
Clone ID Related Accession (Same CDS sequence)	XM_022759122.1
Accession Version	XM_022759122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	serine/threonine-protein kinase ULK1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174272.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCCG TCGGGAAGTT TGAGTTCAAC AGGAAAGACC TGATTGGCCA CGGTGCCTTT 
GCAGTTGTTT TCAAAGGCAG ACATAAAGAG AGACGTGACT GGGAGGTTGC TGTGAAGTGT 
ATCAATAAGA AAAACTTGGC CAAATCCCAG TCTCTGCTTG GTAAAGAAAT CAAAATCTTA 
AAGGAACTCA AACATGAAAA TATTGTTAGA CTACTTGACT ATCAGGAAAT GGGAGGATGT 
GTGTATCTCG TTATGGAGTA TTGCAATGGA GGTGACCTGG CAGAGTACCT TCACTCTAAG 
GGCACGTTAA GTGAGGACAC CATCCGTGTA TTCCTACAGC AGATTGCTCA GGCCATGAAG 
GTCCTGCAGG GTAAAGGCAT CCTCCACAGA GACCTCAAAC CCCAGAACAT CTTGCTCTGC 
CACCCAGAGG GGCGCAGGTC CAGTTCCATC AATACCTGCA TTAAGATTGC TGACTTTGGG 
TTTGCACGTC ATCTCCAGAC AAACACTATG GCAGCCACGC TGTGTGGCTC TCCCATGTAC 
ATGGCTCCTG AGGTCATCAT GTCCCAGAAC TATGATGCCA AGGCTGATCT GTGGAGCATA 
GGCACTATTG TGTACCAGTG TCTGACTGGG AAGGCACCAT TTCACGCTAG CACACCGCAG 
GAGCTCCGTC TCTTTTACGA AAGCAACAGG ACCCTGTTAC CCAGCATCCC AAAGGAGACT 
TCTAGTAACC TAAGACACCT GCTGTTGGGG CTGCTGCAGA GGAACCACAA AGAACGGATC 
AGCTTTGATG AGTTTTTCCA CCATCCGTTC ATGGAGACGA GCTCATCCAC AAAGAAATGC 
TCTCCAGCTC CCATGGTCTC CTATCCCAGC TCAGGCTCAG GCAGTTCTTC TAGTAGCTCC 
TCCACATCCC ATCTGGCCTC ACCTCAACAT TCCGATGGAG AGATGCACCG CCTCCAGCCC 
AAAGCGCAGT ACTCGCCTAC GCAAAACACC GCTGGCTTCC TCCTGAAAGA AATAGCCAAT 
CAAGACAGTA GGAACACCTC ATCTTACACA GATGACTATG TCATGGTGCC TGCCCAGTTT 
CCCAGTGAGT ACACATGTGA AGTGGATGTT GGGTCACCAA TTGAAAGTTG CCTTATATAC 
AGTGGGAGCT CCCTGGTCGC TGAGAGAGGT CCCGGGCCTG CAGGAAAGAC ACCTCCACCC 
TCTCCCCTCC TACACTCTCC CTCCAAGCCC GTTAGCAAGC CCGTTGAATT CGTTGGCCGT 
AAGTGCAGCC CCTCTGTGCC CATTCCTGTC CCAACACAGA TCCAAAACTA CCAGCGTATG 
GAACAGAACC TGCAACCAGT TGGCTTGAAT GGCTCCACCA GGGCCACACT GTGCTGTGCA 
GGCAGCAGCA GTGGAAGCTC CCCACAATGT GGAGGCTGTA GAGCTCCAGG TCCTGGATAT 
GTCCTCAACT CCCCAAGACT GGCAACTGGA GGCGCCCAGC CGCAACCGTG CTCCCCGCTA 
GTGGGAAATA CCCCAAAGAC CTCAGAGCAG ACTCTCCCAC TCAGCACAAG AACTGGACTG 
CTCACTGGCT CTCCTGGCAT GCAGGACACC TCCAGTCCCA AGCAGCAGGT TCAGTCAAGA 
ATGTCAAACC GAATCAGGAC AGTCACAGAC CTGCAATCCC TTGGCACATC TCCTGCTTTC 
CAGACCAACC ACAGCAGGCA ATCTGCCAAT AAGAAGGGTC CTTTTAAAAG ATCGCTGAGC 
ACTGGCAGGC TGTCTGACAT GCTACTGAAG GCGGCCGTTG GAGCTCACCT GTTTGAAGAG 
GGAAGTGATG AGAGCCATAA CTGTGACAAA AGCATGGATA TAACAGCTCC ACCTACTGGT 
TGTAGTAGAG GCTTTCAATG GGCGGACAGC CCACCTCTTG CAGTCTTCAC TATAGGTTCT 
CCAGGCAAAG GAAACACTCC TCCTGATGCC ATGGTGTCAA GGATGTTCTC AGGCTCCCCC 
AGTTACATTC ACTCAACCTG GCTACTTAAC AGTCGCCTTC TCCAGAGAGG AAGCCGTAGA 
AACAGTGAGA CTGAAGCCAT GGAGGCTACT CCACACAGCA GTCCGGTCTT TCACCCTCCA 
GTGCTCGCTG AGGATACACT CATGGAGCAG GCTCATACAG ACGGCCTGAG TGACCTGCGT 
TTCACCTTGG CTTTTGTCCA CTGTGTCATG GAATTGGCTT CTTCAAAAGA CGCAGGGCTG 
GATGCCATCA GCAGTCCCGA TGTTTCCTTT CTAGAGCAGA GCTTGGTGAC AGATCAGATA 
AGCGTTCTGA GTAGAGAATG GAGCTATGCA CAGCAGTTAG TGTTGTACAT GAAGGCTGAA 
GAGTTTCTGT CATCAGCGCT GCACACTGCT AAAGAGAATA TCAAACAAGG CAGACTCCTT 
CCCTCTGCTA CAGTTAAACA AGTGATCAGG AAGCTGAATG AATTGTACAA GAACTGTGTA 
ACATACTGTC GATCCCTCAA CGATCGGCTG CAGACCTTCT TGCTTGACAA ACAGAGGCTT 
ATGGACCGCT TCAATGGACT CACGGCAGAG AAGCTCATCT ACAACCACAC TGTGCACATG 
GTACAGTCTG CTGCTTTAGA TGAGATGTTC CACTATGGCA CCGCATCAGT CCAGCGCTAC 
CATAGAGCCC TTCTGCTGAT GGAGGGTCTG TCCCGAGTCA TCACAGAGCA AAAGGACATT 
GACAGCATAG ACAAATGTAA GCAGTGCATT GAGCGACGCC TTTCTGCTCT GCAGACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022614843.1
CDS	396..3155
Translation	MESVGKFEFNRKDLIGHGAFAVVFKGRHKERRDWEVAVKCINKKNLAKSQSLLGKEIKILKELKHENIVRLLDYQEMGGCVYLVMEYCNGGDLAEYLHSKGTLSEDTIRVFLQQIAQAMKVLQGKGILHRDLKPQNILLCHPEGRRSSSINTCIKIADFGFARHLQTNTMAATLCGSPMYMAPEVIMSQNYDAKADLWSIGTIVYQCLTGKAPFHASTPQELRLFYESNRTLLPSIPKETSSNLRHLLLGLLQRNHKERISFDEFFHHPFMETSSSTKKCSPAPMVSYPSSGSGSSSSSSSTSHLASPQHSDGEMHRLQPKAQYSPTQNTAGFLLKEIANQDSRNTSSYTDDYVMVPAQFPSEYTCEVDVGSPIESCLIYSGSSLVAERGPGPAGKTPPPSPLLHSPSKPVSKPVEFVGRKCSPSVPIPVPTQIQNYQRMEQNLQPVGLNGSTRATLCCAGSSSGSSPQCGGCRAPGPGYVLNSPRLATGGAQPQPCSPLVGNTPKTSEQTLPLSTRTGLLTGSPGMQDTSSPKQQVQSRMSNRIRTVTDLQSLGTSPAFQTNHSRQSANKKGPFKRSLSTGRLSDMLLKAAVGAHLFEEGSDESHNCDKSMDITAPPTGCSRGFQWADSPPLAVFTIGSPGKGNTPPDAMVSRMFSGSPSYIHSTWLLNSRLLQRGSRRNSETEAMEATPHSSPVFHPPVLAEDTLMEQAHTDGLSDLRFTLAFVHCVMELASSKDAGLDAISSPDVSFLEQSLVTDQISVLSREWSYAQQLVLYMKAEEFLSSALHTAKENIKQGRLLPSATVKQVIRKLNELYKNCVTYCRSLNDRLQTFLLDKQRLMDRFNGLTAEKLIYNHTVHMVQSAALDEMFHYGTASVQRYHRALLLMEGLSRVITEQKDIDSIDKCKQCIERRLSALQT

Target ORF information:

RefSeq Version	XM_022759122.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili serine/threonine-protein kinase ULK1-like (LOC111231860), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022759122.1

ORF Insert Sequence:

ATGGAGTCCG TCGGGAAGTT TGAGTTCAAC AGGAAAGACC TGATTGGCCA CGGTGCCTTT 
GCAGTTGTTT TCAAAGGCAG ACATAAAGAG AGACGTGACT GGGAGGTTGC TGTGAAGTGT 
ATCAATAAGA AAAACTTGGC CAAATCCCAG TCTCTGCTTG GTAAAGAAAT CAAAATCTTA 
AAGGAACTCA AACATGAAAA TATTGTTAGA CTACTTGACT ATCAGGAAAT GGGAGGATGT 
GTGTATCTCG TTATGGAGTA TTGCAATGGA GGTGACCTGG CAGAGTACCT TCACTCTAAG 
GGCACGTTAA GTGAGGACAC CATCCGTGTA TTCCTACAGC AGATTGCTCA GGCCATGAAG 
GTCCTGCAGG GTAAAGGCAT CCTCCACAGA GACCTCAAAC CCCAGAACAT CTTGCTCTGC 
CACCCAGAGG GGCGCAGGTC CAGTTCCATC AATACCTGCA TTAAGATTGC TGACTTTGGG 
TTTGCACGTC ATCTCCAGAC AAACACTATG GCAGCCACGC TGTGTGGCTC TCCCATGTAC 
ATGGCTCCTG AGGTCATCAT GTCCCAGAAC TATGATGCCA AGGCTGATCT GTGGAGCATA 
GGCACTATTG TGTACCAGTG TCTGACTGGG AAGGCACCAT TTCACGCTAG CACACCGCAG 
GAGCTCCGTC TCTTTTACGA AAGCAACAGG ACCCTGTTAC CCAGCATCCC AAAGGAGACT 
TCTAGTAACC TAAGACACCT GCTGTTGGGG CTGCTGCAGA GGAACCACAA AGAACGGATC 
AGCTTTGATG AGTTTTTCCA CCATCCGTTC ATGGAGACGA GCTCATCCAC AAAGAAATGC 
TCTCCAGCTC CCATGGTCTC CTATCCCAGC TCAGGCTCAG GCAGTTCTTC TAGTAGCTCC 
TCCACATCCC ATCTGGCCTC ACCTCAACAT TCCGATGGAG AGATGCACCG CCTCCAGCCC 
AAAGCGCAGT ACTCGCCTAC GCAAAACACC GCTGGCTTCC TCCTGAAAGA AATAGCCAAT 
CAAGACAGTA GGAACACCTC ATCTTACACA GATGACTATG TCATGGTGCC TGCCCAGTTT 
CCCAGTGAGT ACACATGTGA AGTGGATGTT GGGTCACCAA TTGAAAGTTG CCTTATATAC 
AGTGGGAGCT CCCTGGTCGC TGAGAGAGGT CCCGGGCCTG CAGGAAAGAC ACCTCCACCC 
TCTCCCCTCC TACACTCTCC CTCCAAGCCC GTTAGCAAGC CCGTTGAATT CGTTGGCCGT 
AAGTGCAGCC CCTCTGTGCC CATTCCTGTC CCAACACAGA TCCAAAACTA CCAGCGTATG 
GAACAGAACC TGCAACCAGT TGGCTTGAAT GGCTCCACCA GGGCCACACT GTGCTGTGCA 
GGCAGCAGCA GTGGAAGCTC CCCACAATGT GGAGGCTGTA GAGCTCCAGG TCCTGGATAT 
GTCCTCAACT CCCCAAGACT GGCAACTGGA GGCGCCCAGC CGCAACCGTG CTCCCCGCTA 
GTGGGAAATA CCCCAAAGAC CTCAGAGCAG ACTCTCCCAC TCAGCACAAG AACTGGACTG 
CTCACTGGCT CTCCTGGCAT GCAGGACACC TCCAGTCCCA AGCAGCAGGT TCAGTCAAGA 
ATGTCAAACC GAATCAGGAC AGTCACAGAC CTGCAATCCC TTGGCACATC TCCTGCTTTC 
CAGACCAACC ACAGCAGGCA ATCTGCCAAT AAGAAGGGTC CTTTTAAAAG ATCGCTGAGC 
ACTGGCAGGC TGTCTGACAT GCTACTGAAG GCGGCCGTTG GAGCTCACCT GTTTGAAGAG 
GGAAGTGATG AGAGCCATAA CTGTGACAAA AGCATGGATA TAACAGCTCC ACCTACTGGT 
TGTAGTAGAG GCTTTCAATG GGCGGACAGC CCACCTCTTG CAGTCTTCAC TATAGGTTCT 
CCAGGCAAAG GAAACACTCC TCCTGATGCC ATGGTGTCAA GGATGTTCTC AGGCTCCCCC 
AGTTACATTC ACTCAACCTG GCTACTTAAC AGTCGCCTTC TCCAGAGAGG AAGCCGTAGA 
AACAGTGAGA CTGAAGCCAT GGAGGCTACT CCACACAGCA GTCCGGTCTT TCACCCTCCA 
GTGCTCGCTG AGGATACACT CATGGAGCAG GCTCATACAG ACGGCCTGAG TGACCTGCGT 
TTCACCTTGG CTTTTGTCCA CTGTGTCATG GAATTGGCTT CTTCAAAAGA CGCAGGGCTG 
GATGCCATCA GCAGTCCCGA TGTTTCCTTT CTAGAGCAGA GCTTGGTGAC AGATCAGATA 
AGCGTTCTGA GTAGAGAATG GAGCTATGCA CAGCAGTTAG TGTTGTACAT GAAGGCTGAA 
GAGTTTCTGT CATCAGCGCT GCACACTGCT AAAGAGAATA TCAAACAAGG CAGACTCCTT 
CCCTCTGCTA CAGTTAAACA AGTGATCAGG AAGCTGAATG AATTGTACAA GAACTGTGTA 
ACATACTGTC GATCCCTCAA CGATCGGCTG CAGACCTTCT TGCTTGACAA ACAGAGGCTT 
ATGGACCGCT TCAATGGACT CACGGCAGAG AAGCTCATCT ACAACCACAC TGTGCACATG 
GTACAGTCTG CTGCTTTAGA TGAGATGTTC CACTATGGCA CCGCATCAGT CCAGCGCTAC 
CATAGAGCCC TTCTGCTGAT GGAGGGTCTG TCCCGAGTCA TCACAGAGCA AAAGGACATT 
GACAGCATAG ACAAATGTAA GCAGTGCATT GAGCGACGCC TTTCTGCTCT GCAGACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC111231860 ( XM_022759128.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022614849.1 Seriola dumerili serine/threonine-protein kinase ULK1-like (LOC111231860), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe208165
Clone ID Related Accession (Same CDS sequence)	XM_022759128.1
Accession Version	XM_022759128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	serine/threonine-protein kinase ULK1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174272.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCCG TCGGGAAGTT TGAGTTCAAC AGGAAAGACC TGATTGGCCA CGGTGCCTTT 
GCAGTTGTTT TCAAAGGCAG ACATAAAGAG AGACGTGACT GGGAGGTTGC TGTGAAGTGT 
ATCAATAAGA AAAACTTGGC CAAATCCCAG TCTCTGCTTG GTAAAGAAAT CAAAATCTTA 
AAGGAACTCA AACATGAAAA TATTGTTAGA CTACTTGACT ATCAGGAAAT GGGAGGATGT 
GTGTATCTCG TTATGGAGTA TTGCAATGGA GGTGACCTGG CAGAGTACCT TCACTCTAAG 
GGCACGTTAA GTGAGGACAC CATCCGTGTA TTCCTACAGC AGATTGCTCA GGCCATGAAG 
GTCCTGCAGG GTAAAGGCAT CCTCCACAGA GACCTCAAAC CCCAGAACAT CTTGCTCTGC 
CACCCAGAGG GGCGCAGGTC CAGTTCCATC AATACCTGCA TTAAGATTGC TGACTTTGGG 
TTTGCACGTC ATCTCCAGAC AAACACTATG GCAGCCACGC TGTGTGGCTC TCCCATGTAC 
ATGGCTCCTG AGGTCATCAT GTCCCAGAAC TATGATGCCA AGGCTGATCT GTGGAGCATA 
GGCACTATTG TGTACCAGTG TCTGACTGGG AAGGCACCAT TTCACGCTAG CACACCGCAG 
GAGCTCCGTC TCTTTTACGA AAGCAACAGG ACCCTGTTAC CCAGCATCCC AAAGGAGACT 
TCTAGTAACC TAAGACACCT GCTGTTGGGG CTGCTGCAGA GGAACCACAA AGAACGGATC 
AGCTTTGATG AGTTTTTCCA CCATCCGTTC ATGGAGACGA GCTCATCCAC AAAGAAATGC 
TCTCCAGCTC CCATGGTCTC CTATCCCAGC TCAGGCTCAG GCAGTTCTTC TAGTAGCTCC 
TCCACATCCC ATCTGGCCTC ACCTCAACAT TCCGATGGAG AGATGCACCG CCTCCAGCCC 
AAAGCGCAGT ACTCGCCTAC GCAAAACACC GCTGGCTTCC TCCTGAAAGA AATAGCCAAT 
CAAGACAGTA GGAACACCTC ATCTTACACA GATGACTATG TCATGGTGCC TGCCCAGTTT 
CCCAGTGAGT ACACATGTGA AGTGGATGTT GGGTCACCAA TTGAAAGTTG CCTTATATAC 
AGTGGGAGCT CCCTGGTCGC TGAGAGAGGT CCCGGGCCTG CAGGAAAGAC ACCTCCACCC 
TCTCCCCTCC TACACTCTCC CTCCAAGCCC GTTAGCAAGC CCGTTGAATT CGTTGGCCGT 
AAGTGCAGCC CCTCTGTGCC CATTCCTGTC CCAACACAGA TCCAAAACTA CCAGCGTATG 
GAACAGAACC TGCAACCAGT TGGCTTGAAT GGCTCCACCA GGGCCACACT GTGCTGTGCA 
GGCAGCAGCA GTGGAAGCTC CCCACAATGT GGAGGCTGTA GAGCTCCAGG TCCTGGATAT 
GTCCTCAACT CCCCAAGACT GGCAACTGGA GGCGCCCAGC CGCAACCGTG CTCCCCGCTA 
GTGGGAAATA CCCCAAAGAC CTCAGAGCAG ACTCTCCCAC TCAGCACAAG AACTGGACTG 
CTCACTGGCT CTCCTGGCAT GCAGGACACC TCCAGTCCCA AGCAGGTTCA GTCAAGAATG 
TCAAACCGAA TCAGGACAGT CACAGACCTG CAATCCCTTG GCACATCTCC TGCTTTCCAG 
ACCAACCACA GCAGGCAATC TGCCAATAAG AAGGGTCCTT TTAAAAGATC GCTGAGCACT 
GGCAGGCTGT CTGACATGCT ACTGAAGGCG GCCGTTGGAG CTCACCTGTT TGAAGAGGGA 
AGTGATGAGA GCCATAACTG TGACAAAAGC ATGGATATAA CAGCTCCACC TACTGGTTGT 
AGTAGAGGCT TTCAATGGGC GGACAGCCCA CCTCTTGCAG TCTTCACTAT AGGTTCTCCA 
GGCAAAGGAA ACACTCCTCC TGATGCCATG GTGTCAAGGA TGTTCTCAGG CTCCCCCAGT 
TACATTCACT CAACCTGGCT ACTTAACAGT CGCCTTCTCC AGAGAGGAAG CCGTAGAAAC 
AGTGAGACTG AAGCCATGGA GGCTACTCCA CACAGCAGTC CGGTCTTTCA CCCTCCAGTG 
CTCGCTGAGG ATACACTCAT GGAGCAGGCT CATACAGACG GCCTGAGTGA CCTGCGTTTC 
ACCTTGGCTT TTGTCCACTG TGTCATGGAA TTGGCTTCTT CAAAAGACGC AGGGCTGGAT 
GCCATCAGCA GTCCCGATGT TTCCTTTCTA GAGCAGAGCT TGGTGACAGA TCAGATAAGC 
GTTCTGAGTA GAGAATGGAG CTATGCACAG CAGTTAGTGT TGTACATGAA GGCTGAAGAG 
TTTCTGTCAT CAGCGCTGCA CACTGCTAAA GAGAATATCA AACAAGGCAG ACTCCTTCCC 
TCTGCTACAG TTAAACAAGT GATCAGGAAG CTGAATGAAT TGTACAAGAA CTGTGTAACA 
TACTGTCGAT CCCTCAACGA TCGGCTGCAG ACCTTCTTGC TTGACAAACA GAGGCTTATG 
GACCGCTTCA ATGGACTCAC GGCAGAGAAG CTCATCTACA ACCACACTGT GCACATGGTA 
CAGTCTGCTG CTTTAGATGA GATGTTCCAC TATGGCACCG CATCAGTCCA GCGCTACCAT 
AGAGCCCTTC TGCTGATGGA GGGTCTGTCC CGAGTCATCA CAGAGCAAAA GGACATTGAC 
AGCATAGACA AATGTAAGCA GTGCATTGAG CGACGCCTTT CTGCTCTGCA GACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022614849.1
CDS	396..3152
Translation	MESVGKFEFNRKDLIGHGAFAVVFKGRHKERRDWEVAVKCINKKNLAKSQSLLGKEIKILKELKHENIVRLLDYQEMGGCVYLVMEYCNGGDLAEYLHSKGTLSEDTIRVFLQQIAQAMKVLQGKGILHRDLKPQNILLCHPEGRRSSSINTCIKIADFGFARHLQTNTMAATLCGSPMYMAPEVIMSQNYDAKADLWSIGTIVYQCLTGKAPFHASTPQELRLFYESNRTLLPSIPKETSSNLRHLLLGLLQRNHKERISFDEFFHHPFMETSSSTKKCSPAPMVSYPSSGSGSSSSSSSTSHLASPQHSDGEMHRLQPKAQYSPTQNTAGFLLKEIANQDSRNTSSYTDDYVMVPAQFPSEYTCEVDVGSPIESCLIYSGSSLVAERGPGPAGKTPPPSPLLHSPSKPVSKPVEFVGRKCSPSVPIPVPTQIQNYQRMEQNLQPVGLNGSTRATLCCAGSSSGSSPQCGGCRAPGPGYVLNSPRLATGGAQPQPCSPLVGNTPKTSEQTLPLSTRTGLLTGSPGMQDTSSPKQVQSRMSNRIRTVTDLQSLGTSPAFQTNHSRQSANKKGPFKRSLSTGRLSDMLLKAAVGAHLFEEGSDESHNCDKSMDITAPPTGCSRGFQWADSPPLAVFTIGSPGKGNTPPDAMVSRMFSGSPSYIHSTWLLNSRLLQRGSRRNSETEAMEATPHSSPVFHPPVLAEDTLMEQAHTDGLSDLRFTLAFVHCVMELASSKDAGLDAISSPDVSFLEQSLVTDQISVLSREWSYAQQLVLYMKAEEFLSSALHTAKENIKQGRLLPSATVKQVIRKLNELYKNCVTYCRSLNDRLQTFLLDKQRLMDRFNGLTAEKLIYNHTVHMVQSAALDEMFHYGTASVQRYHRALLLMEGLSRVITEQKDIDSIDKCKQCIERRLSALQT

Target ORF information:

RefSeq Version	XM_022759128.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili serine/threonine-protein kinase ULK1-like (LOC111231860), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022759128.1

ORF Insert Sequence:

ATGGAGTCCG TCGGGAAGTT TGAGTTCAAC AGGAAAGACC TGATTGGCCA CGGTGCCTTT 
GCAGTTGTTT TCAAAGGCAG ACATAAAGAG AGACGTGACT GGGAGGTTGC TGTGAAGTGT 
ATCAATAAGA AAAACTTGGC CAAATCCCAG TCTCTGCTTG GTAAAGAAAT CAAAATCTTA 
AAGGAACTCA AACATGAAAA TATTGTTAGA CTACTTGACT ATCAGGAAAT GGGAGGATGT 
GTGTATCTCG TTATGGAGTA TTGCAATGGA GGTGACCTGG CAGAGTACCT TCACTCTAAG 
GGCACGTTAA GTGAGGACAC CATCCGTGTA TTCCTACAGC AGATTGCTCA GGCCATGAAG 
GTCCTGCAGG GTAAAGGCAT CCTCCACAGA GACCTCAAAC CCCAGAACAT CTTGCTCTGC 
CACCCAGAGG GGCGCAGGTC CAGTTCCATC AATACCTGCA TTAAGATTGC TGACTTTGGG 
TTTGCACGTC ATCTCCAGAC AAACACTATG GCAGCCACGC TGTGTGGCTC TCCCATGTAC 
ATGGCTCCTG AGGTCATCAT GTCCCAGAAC TATGATGCCA AGGCTGATCT GTGGAGCATA 
GGCACTATTG TGTACCAGTG TCTGACTGGG AAGGCACCAT TTCACGCTAG CACACCGCAG 
GAGCTCCGTC TCTTTTACGA AAGCAACAGG ACCCTGTTAC CCAGCATCCC AAAGGAGACT 
TCTAGTAACC TAAGACACCT GCTGTTGGGG CTGCTGCAGA GGAACCACAA AGAACGGATC 
AGCTTTGATG AGTTTTTCCA CCATCCGTTC ATGGAGACGA GCTCATCCAC AAAGAAATGC 
TCTCCAGCTC CCATGGTCTC CTATCCCAGC TCAGGCTCAG GCAGTTCTTC TAGTAGCTCC 
TCCACATCCC ATCTGGCCTC ACCTCAACAT TCCGATGGAG AGATGCACCG CCTCCAGCCC 
AAAGCGCAGT ACTCGCCTAC GCAAAACACC GCTGGCTTCC TCCTGAAAGA AATAGCCAAT 
CAAGACAGTA GGAACACCTC ATCTTACACA GATGACTATG TCATGGTGCC TGCCCAGTTT 
CCCAGTGAGT ACACATGTGA AGTGGATGTT GGGTCACCAA TTGAAAGTTG CCTTATATAC 
AGTGGGAGCT CCCTGGTCGC TGAGAGAGGT CCCGGGCCTG CAGGAAAGAC ACCTCCACCC 
TCTCCCCTCC TACACTCTCC CTCCAAGCCC GTTAGCAAGC CCGTTGAATT CGTTGGCCGT 
AAGTGCAGCC CCTCTGTGCC CATTCCTGTC CCAACACAGA TCCAAAACTA CCAGCGTATG 
GAACAGAACC TGCAACCAGT TGGCTTGAAT GGCTCCACCA GGGCCACACT GTGCTGTGCA 
GGCAGCAGCA GTGGAAGCTC CCCACAATGT GGAGGCTGTA GAGCTCCAGG TCCTGGATAT 
GTCCTCAACT CCCCAAGACT GGCAACTGGA GGCGCCCAGC CGCAACCGTG CTCCCCGCTA 
GTGGGAAATA CCCCAAAGAC CTCAGAGCAG ACTCTCCCAC TCAGCACAAG AACTGGACTG 
CTCACTGGCT CTCCTGGCAT GCAGGACACC TCCAGTCCCA AGCAGGTTCA GTCAAGAATG 
TCAAACCGAA TCAGGACAGT CACAGACCTG CAATCCCTTG GCACATCTCC TGCTTTCCAG 
ACCAACCACA GCAGGCAATC TGCCAATAAG AAGGGTCCTT TTAAAAGATC GCTGAGCACT 
GGCAGGCTGT CTGACATGCT ACTGAAGGCG GCCGTTGGAG CTCACCTGTT TGAAGAGGGA 
AGTGATGAGA GCCATAACTG TGACAAAAGC ATGGATATAA CAGCTCCACC TACTGGTTGT 
AGTAGAGGCT TTCAATGGGC GGACAGCCCA CCTCTTGCAG TCTTCACTAT AGGTTCTCCA 
GGCAAAGGAA ACACTCCTCC TGATGCCATG GTGTCAAGGA TGTTCTCAGG CTCCCCCAGT 
TACATTCACT CAACCTGGCT ACTTAACAGT CGCCTTCTCC AGAGAGGAAG CCGTAGAAAC 
AGTGAGACTG AAGCCATGGA GGCTACTCCA CACAGCAGTC CGGTCTTTCA CCCTCCAGTG 
CTCGCTGAGG ATACACTCAT GGAGCAGGCT CATACAGACG GCCTGAGTGA CCTGCGTTTC 
ACCTTGGCTT TTGTCCACTG TGTCATGGAA TTGGCTTCTT CAAAAGACGC AGGGCTGGAT 
GCCATCAGCA GTCCCGATGT TTCCTTTCTA GAGCAGAGCT TGGTGACAGA TCAGATAAGC 
GTTCTGAGTA GAGAATGGAG CTATGCACAG CAGTTAGTGT TGTACATGAA GGCTGAAGAG 
TTTCTGTCAT CAGCGCTGCA CACTGCTAAA GAGAATATCA AACAAGGCAG ACTCCTTCCC 
TCTGCTACAG TTAAACAAGT GATCAGGAAG CTGAATGAAT TGTACAAGAA CTGTGTAACA 
TACTGTCGAT CCCTCAACGA TCGGCTGCAG ACCTTCTTGC TTGACAAACA GAGGCTTATG 
GACCGCTTCA ATGGACTCAC GGCAGAGAAG CTCATCTACA ACCACACTGT GCACATGGTA 
CAGTCTGCTG CTTTAGATGA GATGTTCCAC TATGGCACCG CATCAGTCCA GCGCTACCAT 
AGAGCCCTTC TGCTGATGGA GGGTCTGTCC CGAGTCATCA CAGAGCAAAA GGACATTGAC 
AGCATAGACA AATGTAAGCA GTGCATTGAG CGACGCCTTT CTGCTCTGCA GACTTAA