thoc1 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	thoc1
Entrez Gene ID	111231148
Full Name	THO complex 1
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022758161.1	XP_022613882.1	THO complex subunit 1

Pathways from BioSystems
Gene Ontology

The following thoc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the thoc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe204110	XM_022758161.1 Latest version!	Seriola dumerili THO complex 1 (thoc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

thoc1 ( XM_022758161.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022613882.1 Seriola dumerili THO complex 1 (thoc1), mRNA.

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CloneID	OSe204110
Clone ID Related Accession (Same CDS sequence)	XM_022758161.1
Accession Version	XM_022758161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1965bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	THO complex subunit 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGCCGT CCTCATTTAA TTTTGTCGAG GCTAAAGACA AATTTACGGC TTCTGCCAGA 
TTTGCCCTAA CAAGTAAAAC CAGCAAACCT TTGATAAACG CTTTCAATCA GATCCCCGGC 
AATGAAACGG AAAAGAAAAC CACACTTGAC CAAGCTCTGA GGAGTGTTCT TGGTGATCAG 
ATTATTGAGC ATAAGGCGAG CTGTGATGAC TACCTGTCTC TGATCTACCT GAGCATTGAC 
GCCGTTACAG AGGGTATCTG CTCTGCTACC ACACCTTTCC TTCTGCTGGG AGATGTACTG 
GACTGCCTTC CTCTTGACCA GTGTGACAAA ATATTCTCTT TTGTCGAGGA GAATGTCTCC 
ACCTGGAAGT CGAACTCCTT TTACACTGCT GGGAAGAACT ACTTGTTGAG GATGTGTAAT 
GACCTTTTGA GGAGGCTGTC CAAATCTCAG AATACAGTGT TCTGTGGGCG AATCCAGCTT 
TTCCTGGCAC GCCTGTTCCC TTTGTCTGAG AAATCAGGTC TCAATCTACA GAGCCAGTTT 
AATCTGGACA ATATAACAGT GTTCAATAAA AATGAACAAG AAAGTACTCT CGGCCAGAAG 
CACACAGAAG AAAAGGAAGA AGGTATGGAG GTGGAGGAAG GAGAAATGGG GGAGGATGAT 
ACTCCTGCAC CATGCTCCAT TCCGATTGAC TACAACTTGT ACAGGAAGTT CTGGACACTG 
CAGGACTACT TCAGAAACCC TGTGCAGTGC TACGATAAAT TCTCTTGGAT GACATTCCTT 
AAGTACTCAG ATGAGACGCT GGCAGTATTC AAGAGCTACA AGCTGGATGA CATGCAGGCC 
TCTAAGAGGA AACTGGAGGA GCTTAGAGCA TCTGATGGAG AGCATGTCTA CTTTGCTAAG 
TTCCTCACAA GTGAGAAGCT GATGGATTTG CAGCTCAGTG ACAGCAATTT CAGGCGACAC 
ATTCTACTTC AGTACCTCAT TCTCTTCCAG TACTTGAAGG GTCAGGTCAA ATTCAAAAGC 
TCCAGCTCTG TTCTGAATGA TGACCAGACT ACATGGATAG AGGAGACAAC TAAACTGGTT 
TATCAGCTGC TGAGAGAAAT CCCTCCTGAT GGAGACAAGT TTGCCACCAT GGTTGAGCAT 
ATCCTCAACA CAGAGGAGAA CTGGAATGTC TGGAAAAATG AGGGATGCCC AAGCTTTGTG 
AAAGAAAGGA CGGTAGATGA CAAACCCAAA AGACCGACCA GGAAAAGACA AGCACCAGAA 
GACTTCCTGG GAAAAGGGCC GGACCGCAAG ATCTTCATGG GAAATGATGA GCTGACTCGG 
CTGTGGAACC TGAACCAGGA CAACATGGAA GCCTGCAAGT CAGACAGCAG AGAGTTCATG 
CCATCACTGG ATGAATTCTT TGCAGAAGCC ATAGAACAGG CCGACCCTGC TAACATGGTG 
GAGGAGGAGT ACAAGGTTGT ACGGAACCCA AACTATGGCT GGCGCGCTCT GAGGCTCCTG 
TCCAGGAGAA GTCCACACTT CTTTCAACCG ACTAACCAGA AGTTCAAGAG CCTGGCAGAC 
TATCTAGATA GCATGGTCAG CAAACTGGCC AAAGAACTGC CGAAGGATAT CCCTTCTGAA 
GAGATCAAGA CAGGAGAAGA GGATGATGAC GATAATGGAG ACAATCTTCT CAAAGACAGC 
AATGACAGTC CGAGCATACA GAGCAAGCTG GTGACAAACC AGCAGATGGA TGACATTGCA 
GCCAAACTGG GCGCTCAGTG GAAGATGCTG GCTTCTCATT TGGAAATGAA GGCGGCAGAG 
TTGCGAGAGA TCGAAACAGA TAGTGAAGAT GTTGACATGC AGGCCAAACT GCTGCTTGTA 
GCCTGGCAGG ACAGAGAGGG AACACAAGCC ACTGTGGAGA GCCTGATCAC AGCTCTTAAT 
GCTGCAGGAT TCTCTCAGAT TGCAGATGGC CTCAATGAGG CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022613882.1
CDS	18..1982
Translation	MSPSSFNFVEAKDKFTASARFALTSKTSKPLINAFNQIPGNETEKKTTLDQALRSVLGDQIIEHKASCDDYLSLIYLSIDAVTEGICSATTPFLLLGDVLDCLPLDQCDKIFSFVEENVSTWKSNSFYTAGKNYLLRMCNDLLRRLSKSQNTVFCGRIQLFLARLFPLSEKSGLNLQSQFNLDNITVFNKNEQESTLGQKHTEEKEEGMEVEEGEMGEDDTPAPCSIPIDYNLYRKFWTLQDYFRNPVQCYDKFSWMTFLKYSDETLAVFKSYKLDDMQASKRKLEELRASDGEHVYFAKFLTSEKLMDLQLSDSNFRRHILLQYLILFQYLKGQVKFKSSSSVLNDDQTTWIEETTKLVYQLLREIPPDGDKFATMVEHILNTEENWNVWKNEGCPSFVKERTVDDKPKRPTRKRQAPEDFLGKGPDRKIFMGNDELTRLWNLNQDNMEACKSDSREFMPSLDEFFAEAIEQADPANMVEEEYKVVRNPNYGWRALRLLSRRSPHFFQPTNQKFKSLADYLDSMVSKLAKELPKDIPSEEIKTGEEDDDDNGDNLLKDSNDSPSIQSKLVTNQQMDDIAAKLGAQWKMLASHLEMKAAELREIETDSEDVDMQAKLLLVAWQDREGTQATVESLITALNAAGFSQIADGLNEA

Target ORF information:

RefSeq Version	XM_022758161.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili THO complex 1 (thoc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022758161.1

ORF Insert Sequence:

ATGTCGCCGT CCTCATTTAA TTTTGTCGAG GCTAAAGACA AATTTACGGC TTCTGCCAGA 
TTTGCCCTAA CAAGTAAAAC CAGCAAACCT TTGATAAACG CTTTCAATCA GATCCCCGGC 
AATGAAACGG AAAAGAAAAC CACACTTGAC CAAGCTCTGA GGAGTGTTCT TGGTGATCAG 
ATTATTGAGC ATAAGGCGAG CTGTGATGAC TACCTGTCTC TGATCTACCT GAGCATTGAC 
GCCGTTACAG AGGGTATCTG CTCTGCTACC ACACCTTTCC TTCTGCTGGG AGATGTACTG 
GACTGCCTTC CTCTTGACCA GTGTGACAAA ATATTCTCTT TTGTCGAGGA GAATGTCTCC 
ACCTGGAAGT CGAACTCCTT TTACACTGCT GGGAAGAACT ACTTGTTGAG GATGTGTAAT 
GACCTTTTGA GGAGGCTGTC CAAATCTCAG AATACAGTGT TCTGTGGGCG AATCCAGCTT 
TTCCTGGCAC GCCTGTTCCC TTTGTCTGAG AAATCAGGTC TCAATCTACA GAGCCAGTTT 
AATCTGGACA ATATAACAGT GTTCAATAAA AATGAACAAG AAAGTACTCT CGGCCAGAAG 
CACACAGAAG AAAAGGAAGA AGGTATGGAG GTGGAGGAAG GAGAAATGGG GGAGGATGAT 
ACTCCTGCAC CATGCTCCAT TCCGATTGAC TACAACTTGT ACAGGAAGTT CTGGACACTG 
CAGGACTACT TCAGAAACCC TGTGCAGTGC TACGATAAAT TCTCTTGGAT GACATTCCTT 
AAGTACTCAG ATGAGACGCT GGCAGTATTC AAGAGCTACA AGCTGGATGA CATGCAGGCC 
TCTAAGAGGA AACTGGAGGA GCTTAGAGCA TCTGATGGAG AGCATGTCTA CTTTGCTAAG 
TTCCTCACAA GTGAGAAGCT GATGGATTTG CAGCTCAGTG ACAGCAATTT CAGGCGACAC 
ATTCTACTTC AGTACCTCAT TCTCTTCCAG TACTTGAAGG GTCAGGTCAA ATTCAAAAGC 
TCCAGCTCTG TTCTGAATGA TGACCAGACT ACATGGATAG AGGAGACAAC TAAACTGGTT 
TATCAGCTGC TGAGAGAAAT CCCTCCTGAT GGAGACAAGT TTGCCACCAT GGTTGAGCAT 
ATCCTCAACA CAGAGGAGAA CTGGAATGTC TGGAAAAATG AGGGATGCCC AAGCTTTGTG 
AAAGAAAGGA CGGTAGATGA CAAACCCAAA AGACCGACCA GGAAAAGACA AGCACCAGAA 
GACTTCCTGG GAAAAGGGCC GGACCGCAAG ATCTTCATGG GAAATGATGA GCTGACTCGG 
CTGTGGAACC TGAACCAGGA CAACATGGAA GCCTGCAAGT CAGACAGCAG AGAGTTCATG 
CCATCACTGG ATGAATTCTT TGCAGAAGCC ATAGAACAGG CCGACCCTGC TAACATGGTG 
GAGGAGGAGT ACAAGGTTGT ACGGAACCCA AACTATGGCT GGCGCGCTCT GAGGCTCCTG 
TCCAGGAGAA GTCCACACTT CTTTCAACCG ACTAACCAGA AGTTCAAGAG CCTGGCAGAC 
TATCTAGATA GCATGGTCAG CAAACTGGCC AAAGAACTGC CGAAGGATAT CCCTTCTGAA 
GAGATCAAGA CAGGAGAAGA GGATGATGAC GATAATGGAG ACAATCTTCT CAAAGACAGC 
AATGACAGTC CGAGCATACA GAGCAAGCTG GTGACAAACC AGCAGATGGA TGACATTGCA 
GCCAAACTGG GCGCTCAGTG GAAGATGCTG GCTTCTCATT TGGAAATGAA GGCGGCAGAG 
TTGCGAGAGA TCGAAACAGA TAGTGAAGAT GTTGACATGC AGGCCAAACT GCTGCTTGTA 
GCCTGGCAGG ACAGAGAGGG AACACAAGCC ACTGTGGAGA GCCTGATCAC AGCTCTTAAT 
GCTGCAGGAT TCTCTCAGAT TGCAGATGGC CTCAATGAGG CTTAA