sergef cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	sergef
Entrez Gene ID	111229596
Full Name	secretion regulating guanine nucleotide exchange factor
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022755988.1	XP_022611709.1	secretion-regulating guanine nucleotide exchange factor isoform X2
XM_022755987.1	XP_022611708.1	secretion-regulating guanine nucleotide exchange factor isoform X1

Pathways from BioSystems
Gene Ontology

The following sergef gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sergef cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe202359	XM_022755987.1 Latest version!	Seriola dumerili secretion regulating guanine nucleotide exchange factor (sergef), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OSe202360	XM_022755988.1 Latest version!	Seriola dumerili secretion regulating guanine nucleotide exchange factor (sergef), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sergef ( XM_022755987.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022611708.1 Seriola dumerili secretion regulating guanine nucleotide exchange factor (sergef), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OSe202359
Clone ID Related Accession (Same CDS sequence)	XM_022755987.1
Accession Version	XM_022755987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	secretion-regulating guanine nucleotide exchange factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174497.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCA AGTCGCAGCC GGAGATAAGT TTACTCTCTT GGGGGGCGAA CAGCTACGGG 
CAGCTGGGAC AGGGACATGT GGAGGACCAG TCAGCGCCCC GGCTCTCTGA CACTGCTGCT 
TTACAAAGCA GGGCAGTCCG GACTGTGAGC GGCGGCGGGG GTCACTCTGT GTTTGTCACC 
GAGAATGGAG AGGTGTTTGT GTGCGGACAG AACAACAGAG GCCAGCTGGG CCTTGGCCAC 
AATACAGACA TCTCAACTCT TCAACTCTGC CCCAGCCTAA ATCAGACAGT CACAAATGTG 
ACCTGTGGTT GGGATTTTAC TCTTCTTCTC ACTGATTGTG GCCAAGTACT GGCATGTGGC 
TCCAATGCAT TTGGACAACT GGGTGTTGGA CCGACAGTCA GGCACTCTGC AGATCTACTG 
TTTGTTGAGT GTCTGAAGGA TCCAGTGGTG AGCGTAGCAG CAGGACTTAG ACACTCACTC 
GCTGTTACTG ACTCAGGCTG CGTGTATCAA TGGGGAACTG GTCTTTTCAG TCTGGCTAAG 
AGAGCACTCA GTCCTCACCC TATCCCATCG CACCTCTGCT CTAAGGTGCC CTGTCTGGTA 
CCAGGTCTGG ATCAGAAAAC ACCACACCTG GTAGCTGCAG GCTCAGCGCA CTGTGTGTGC 
CTTACAGAAG ACGGTGATTT GTTTCTGTGG GGAAGCAACA AACATGGTCA GCTGACCACC 
ACAGAGCCCT TCCTGTCCTC TCCAACTCTT CTGAAGCGTT CGTTACTGGA TGGAGAGAAA 
GTAATAAATT TGTGGAGCGG CTGGACACAC ATTGTTGCTC AAACAGAGAG TGGAAGAGTG 
TTCACATGGG GCAGAGGAGG TTATGGACAG CTGGGCCGAC AAGCATCAAT CAATCAGAAC 
CCAGAACAGC AGTCAGCTGG TGCTTTAGCA GAAGGTGGAA ACCAGGAGGT TTGCCTCCCT 
GCAGAGGTTA AAGTCCTCAA TGGAGCAACT CAGATCGCAT GTGGATCGGA ACATAACCTT 
GCCATCGTGG GGGGTTGTCT CCTTTCCTGG GGCTGGAACG AACATGGAAT GTGTGGAGAC 
GGTTCACAGA CCGACGTCTT TCAGCCTCAG CTCATATCTG GTCTCAGACC TCTTCTCATT 
GGCTGTGGAG CTGGACACTC TATGGCAGTG TGCGCCGCGG CAACTGGCTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022611708.1
CDS	15..1208
Translation	METKSQPEISLLSWGANSYGQLGQGHVEDQSAPRLSDTAALQSRAVRTVSGGGGHSVFVTENGEVFVCGQNNRGQLGLGHNTDISTLQLCPSLNQTVTNVTCGWDFTLLLTDCGQVLACGSNAFGQLGVGPTVRHSADLLFVECLKDPVVSVAAGLRHSLAVTDSGCVYQWGTGLFSLAKRALSPHPIPSHLCSKVPCLVPGLDQKTPHLVAAGSAHCVCLTEDGDLFLWGSNKHGQLTTTEPFLSSPTLLKRSLLDGEKVINLWSGWTHIVAQTESGRVFTWGRGGYGQLGRQASINQNPEQQSAGALAEGGNQEVCLPAEVKVLNGATQIACGSEHNLAIVGGCLLSWGWNEHGMCGDGSQTDVFQPQLISGLRPLLIGCGAGHSMAVCAAATGS

Target ORF information:

RefSeq Version	XM_022755987.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili secretion regulating guanine nucleotide exchange factor (sergef), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022755987.1

ORF Insert Sequence:

ATGGAAACCA AGTCGCAGCC GGAGATAAGT TTACTCTCTT GGGGGGCGAA CAGCTACGGG 
CAGCTGGGAC AGGGACATGT GGAGGACCAG TCAGCGCCCC GGCTCTCTGA CACTGCTGCT 
TTACAAAGCA GGGCAGTCCG GACTGTGAGC GGCGGCGGGG GTCACTCTGT GTTTGTCACC 
GAGAATGGAG AGGTGTTTGT GTGCGGACAG AACAACAGAG GCCAGCTGGG CCTTGGCCAC 
AATACAGACA TCTCAACTCT TCAACTCTGC CCCAGCCTAA ATCAGACAGT CACAAATGTG 
ACCTGTGGTT GGGATTTTAC TCTTCTTCTC ACTGATTGTG GCCAAGTACT GGCATGTGGC 
TCCAATGCAT TTGGACAACT GGGTGTTGGA CCGACAGTCA GGCACTCTGC AGATCTACTG 
TTTGTTGAGT GTCTGAAGGA TCCAGTGGTG AGCGTAGCAG CAGGACTTAG ACACTCACTC 
GCTGTTACTG ACTCAGGCTG CGTGTATCAA TGGGGAACTG GTCTTTTCAG TCTGGCTAAG 
AGAGCACTCA GTCCTCACCC TATCCCATCG CACCTCTGCT CTAAGGTGCC CTGTCTGGTA 
CCAGGTCTGG ATCAGAAAAC ACCACACCTG GTAGCTGCAG GCTCAGCGCA CTGTGTGTGC 
CTTACAGAAG ACGGTGATTT GTTTCTGTGG GGAAGCAACA AACATGGTCA GCTGACCACC 
ACAGAGCCCT TCCTGTCCTC TCCAACTCTT CTGAAGCGTT CGTTACTGGA TGGAGAGAAA 
GTAATAAATT TGTGGAGCGG CTGGACACAC ATTGTTGCTC AAACAGAGAG TGGAAGAGTG 
TTCACATGGG GCAGAGGAGG TTATGGACAG CTGGGCCGAC AAGCATCAAT CAATCAGAAC 
CCAGAACAGC AGTCAGCTGG TGCTTTAGCA GAAGGTGGAA ACCAGGAGGT TTGCCTCCCT 
GCAGAGGTTA AAGTCCTCAA TGGAGCAACT CAGATCGCAT GTGGATCGGA ACATAACCTT 
GCCATCGTGG GGGGTTGTCT CCTTTCCTGG GGCTGGAACG AACATGGAAT GTGTGGAGAC 
GGTTCACAGA CCGACGTCTT TCAGCCTCAG CTCATATCTG GTCTCAGACC TCTTCTCATT 
GGCTGTGGAG CTGGACACTC TATGGCAGTG TGCGCCGCGG CAACTGGCTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

sergef ( XM_022755988.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022611709.1 Seriola dumerili secretion regulating guanine nucleotide exchange factor (sergef), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OSe202360
Clone ID Related Accession (Same CDS sequence)	XM_022755988.1
Accession Version	XM_022755988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	secretion-regulating guanine nucleotide exchange factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174497.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACCA AGTCGCAGCC GGAGATAAGT TTACTCTCTT GGGGGGCGAA CAGCTACGGG 
CAGCTGGGAC AGGGACATGT GGAGGACCAG TCAGCGCCCC GGCTCTCTGA CACTGCTGCT 
TTACAAAGCA GGGCAGTCCG GACTGTGAGC GGCGGCGGGG GTCACTCTGT GTTTGTCACC 
GAGAATGGAG AGGTGTTTGT GTGCGGACAG AACAACAGAG GCCAGCTGGG CCTTGGCCAC 
AATACAGACA TCTCAACTCT TCAACTCTGC CCCAGCCTAA ATCAGACAGT CACAAATGTG 
ACCTGTGGTT GGGATTTTAC TCTTCTTCTC ACTGATTGTG GCCAAGTACT GGCATGTGGC 
TCCAATGCAT TTGGACAACT GGGTGTTGGA CCGACAGTCA GGCACTCTGC AGATCTACTG 
TTTGTTGAGT GTCTGAAGGA TCCAGTGGTG AGCGTAGCAG CAGGACTTAG ACACTCACTC 
GCTGTTACTG ACTCAGGCTG CGTGTATCAA TGGGGAACTG GTCTTTTCAG TCTGGCTAAG 
AGAGCACTCA GTCCTCACCC TATCCCATCG CACCTCTGCT CTAAGGTGCC CTGTCTGGTA 
CCAGGTCTGG ATCAGAAAAC ACCACACCTG GTAGCTGCAG GCTCAGCGCA CTGTGTGTGC 
CTTACAGAAG ACGGTGATTT GTTTCTGTGG GGAAGCAACA AACATGGTCA GCTGACCACC 
ACAGAGCCCT TCCTGTCCTC TCCAACTCTT CTGAAGCGTT CGTTACTGGA TGGAGAGAAA 
GTAATAAATT TGTGGAGCGG CTGGACACAC ATTGTTGCTC AAACAGAGAG TGGAAGAGTG 
TTCACATGGG GCAGAGGAGG TTATGGACAG CTGGGCCGAC AAGCATCAAT CAATCAGAAC 
CCAGAACAGC AGTCAGCTGG TGCTTTAGCA GAAGGTGGAA ACCAGGAGGT TTGCCTCCCT 
GCAGAGGTTA AAGTCCTCAA TGGAGCAACT CAGATCGCAT GTGGATCGGA ACATAACCTT 
GCCATCGTGG AAGTTCGGTT ATGTGATGAG AAAGCAGAAC CGGTAAAGGG GGAGCTCATG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022611709.1
CDS	15..1097
Translation	METKSQPEISLLSWGANSYGQLGQGHVEDQSAPRLSDTAALQSRAVRTVSGGGGHSVFVTENGEVFVCGQNNRGQLGLGHNTDISTLQLCPSLNQTVTNVTCGWDFTLLLTDCGQVLACGSNAFGQLGVGPTVRHSADLLFVECLKDPVVSVAAGLRHSLAVTDSGCVYQWGTGLFSLAKRALSPHPIPSHLCSKVPCLVPGLDQKTPHLVAAGSAHCVCLTEDGDLFLWGSNKHGQLTTTEPFLSSPTLLKRSLLDGEKVINLWSGWTHIVAQTESGRVFTWGRGGYGQLGRQASINQNPEQQSAGALAEGGNQEVCLPAEVKVLNGATQIACGSEHNLAIVEVRLCDEKAEPVKGELM

Target ORF information:

RefSeq Version	XM_022755988.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili secretion regulating guanine nucleotide exchange factor (sergef), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022755988.1

ORF Insert Sequence:

ATGGAAACCA AGTCGCAGCC GGAGATAAGT TTACTCTCTT GGGGGGCGAA CAGCTACGGG 
CAGCTGGGAC AGGGACATGT GGAGGACCAG TCAGCGCCCC GGCTCTCTGA CACTGCTGCT 
TTACAAAGCA GGGCAGTCCG GACTGTGAGC GGCGGCGGGG GTCACTCTGT GTTTGTCACC 
GAGAATGGAG AGGTGTTTGT GTGCGGACAG AACAACAGAG GCCAGCTGGG CCTTGGCCAC 
AATACAGACA TCTCAACTCT TCAACTCTGC CCCAGCCTAA ATCAGACAGT CACAAATGTG 
ACCTGTGGTT GGGATTTTAC TCTTCTTCTC ACTGATTGTG GCCAAGTACT GGCATGTGGC 
TCCAATGCAT TTGGACAACT GGGTGTTGGA CCGACAGTCA GGCACTCTGC AGATCTACTG 
TTTGTTGAGT GTCTGAAGGA TCCAGTGGTG AGCGTAGCAG CAGGACTTAG ACACTCACTC 
GCTGTTACTG ACTCAGGCTG CGTGTATCAA TGGGGAACTG GTCTTTTCAG TCTGGCTAAG 
AGAGCACTCA GTCCTCACCC TATCCCATCG CACCTCTGCT CTAAGGTGCC CTGTCTGGTA 
CCAGGTCTGG ATCAGAAAAC ACCACACCTG GTAGCTGCAG GCTCAGCGCA CTGTGTGTGC 
CTTACAGAAG ACGGTGATTT GTTTCTGTGG GGAAGCAACA AACATGGTCA GCTGACCACC 
ACAGAGCCCT TCCTGTCCTC TCCAACTCTT CTGAAGCGTT CGTTACTGGA TGGAGAGAAA 
GTAATAAATT TGTGGAGCGG CTGGACACAC ATTGTTGCTC AAACAGAGAG TGGAAGAGTG 
TTCACATGGG GCAGAGGAGG TTATGGACAG CTGGGCCGAC AAGCATCAAT CAATCAGAAC 
CCAGAACAGC AGTCAGCTGG TGCTTTAGCA GAAGGTGGAA ACCAGGAGGT TTGCCTCCCT 
GCAGAGGTTA AAGTCCTCAA TGGAGCAACT CAGATCGCAT GTGGATCGGA ACATAACCTT 
GCCATCGTGG AAGTTCGGTT ATGTGATGAG AAAGCAGAAC CGGTAAAGGG GGAGCTCATG 
TGA