polk cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	polk
Entrez Gene ID	111229321
Full Name	DNA polymerase kappa
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022755609.1	XP_022611330.1	DNA polymerase kappa

Pathways from BioSystems
Gene Ontology

The following polk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the polk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe207873	XM_022755609.1 Latest version!	Seriola dumerili DNA polymerase kappa (polk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

polk ( XM_022755609.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022611330.1 Seriola dumerili DNA polymerase kappa (polk), mRNA.

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CloneID	OSe207873
Clone ID Related Accession (Same CDS sequence)	XM_022755609.1
Accession Version	XM_022755609.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	DNA polymerase kappa
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174272.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAATG GAATTGCATC CACTAAAGGA GAGGGCTTCC TCTCCAGAAT GGCACTTAAT 
GACAACAAGG CCGGTATGGA GGGTCTTGAC AGGGACAAGA TTAACAAGAT CATCATGGAG 
TCATCGAAGG GATCAAGGTT TTACGAGAAT GAGCTGAAGA GAGAGCAGCA GGTGAACCAG 
CGCATTGAGA AAATGATGCT ACAAAAGGCA CAGATCACAG AGCAGCAGTT AAAGAAAGCA 
CAAGCTCAGG TGGAGAAGGT CGCCTCAGCG CTGGAGAGGA GTCGTGATCT AAGCCGTGTG 
ATTGTGCATG TGGACATGGA TGCTTTCTAC GCTGCTGTGG AGATGAGAGA CTGTCCTGAG 
CTGAAGGACA AGCCCATGGC TGTAGGATCC ATGAGCATGC TGTCAACATC TAACTACCAT 
GCCAGGAAGT ACGGGGTCCG AGCCGCCATG CCTGGGTTCA TTGCAAAGAA ACTCTGCCCC 
AACTTAGTCA TCGTTCCAAC TAACTTTGAT AAATACAGAG CTGTGAGTGA TGAGATCCGG 
GAAATTTTTG CAGACTATGA CCCTCACTTC CAGCCATTGA GCCTGGATGA AGCCTATCTG 
GATTTTACAG ACCACCTGGA CTACAGGCAG AGCTGGCCGG AGTCCTCTCG TACACATCAC 
TTCCGCGCCA GCAGCACCGC TACAGGTGAA GAGCAGAGTG AACTTCCCCA GGAGGCACTG 
CCAGAGGTGA AAGACCTGTC TCCTGTCCTG TTTGAGGACA GCCCGAGCAC CTCTCCCTGC 
TTGCTGGGCT CTGGTGGTGG TGGTGGTGCA TTCGAGGTGT TTGGGACGTC TGTGGAGGAG 
GCTGTGAGAG AGATGCGCTT CCGCATTGAG CAGAAGACCA TGCTGACTGC CAGTGCAGGA 
ATTGCTCCAA ACATGATGCT TGCCAAGGTG TGCAGTGACA AGAACAAGCC CAACGGCCAG 
TACAGACTCC CCTCCACCAG AGAGGCAGTC ATGGACTTCG TCCATAATCT TCCAGTTCGC 
AAAGTTTCTG GCATTGGGAA AGTGAGTGAG AAGATGCTCA ATGCTTTGGG AATCAGTAGC 
TGTTCTCACC TTGGCCAGCA GATGGCACTG CTGTCACTGT TGTTCTCAGA GACAGCCTGG 
CATCATTTCA TGGAGGTTTC CCTGGGTCTG AGCTCCACAT ATATACCGAG GCATGAGGAC 
AGAAAAAGCA TGAGCACGGA AAGGACATTT AAAGAACTGA GCAAAGCTGA GGAACAGTTT 
TCTTTATGCA GGGAGCTCTG TGAAGATTTA GCAGAAGACA TGAAGAAAGA AGATCTGAAG 
GGTAAAACCG TAACACTGAA GCTTAAGAAC GTGAACTTTG AGGTGAAAAC CAGGGCGCTG 
ACGCTGCTGT GTGCTGTTGC TACAGTGGAT GAGATCTTTG CTGTTGCTAA GGACCTACTG 
AAAACGGAAA TAGAAAATGA AAGCCCCCAG CCACTCAGAC TGAGGCTCAT GGGTGTTCGG 
ATTTCTGCTT TTGTCAGTTC GGATGATAAA AAGCCCCTGC AGAGGAGCAT CATTGGCTTT 
CTTCAGCCAG GAAAGACAGA CTCCAGTGGC TCTATCCAAG GAATGCACCA AAACCCTGAG 
AAAGAGCGTC TCACCAATCA CTCCCTCCAA ACCTGGGCCC TTACTTGTCC TCCCCCAGTC 
CAGAAGTGCC AGAAACAAGA GGAAGTTCCC TGGCGGAGCA AGCAGAAGGG GTTAGAGGGA 
GGTCAGACTG GTGAGGAACC CCAACAGTCT TTCTTCCAAA GGGCCCATGC CAAAAGATTG 
CAACTCCAGG CAGCGAGGTC AAGCACACAA GAGGAAGGAC ACGGGGAGAA TGTTTCTGTC 
ATTAATTCCA TAGATTCTGA TGCTCAAAAG GGTGTAGAGT CATCATCAAA AGCGCACAAG 
AGTACAGCCT CAGATCTCCC AGAAAACGAC TTTGTTGAGG CCCAGGCATC CACATCAGGC 
TGTGGTGGCA CAGCGTCAGA GAGCCTCACC TGTCCCGTGT GTTTCAAGCA GGTGGAGACG 
ACTGATTTGA ATGTCTTCAA CAGACATATA GACCAGTGTC TCAGCGAATC TTCACGAAAA 
CCAAATCGAA GCACAGATTC GGACTCAGAG TCAGATTTGG ACTTAGACAT TGTTCGTAAC 
GGTTGTAAGG TGCTGGAAGA ATGGAGGGAG GAGTGTGAGG TTAATGAGGA GAAGAACAGT 
GAAGAGTTGG AGTCGAACAG AGTCGATCCT CAGTGGCAAC ACGGCTTGAA AAACGATCCC 
ACTCAAACGG TTTTGTTGAT CAATGGTGAC AAGGAAACCG TGACCTCGCA ACAGCCTCAG 
TCATGTAATG ATAAAGGCCC TGTCTTCATC TGCCCAGTGT GTCAGCTGAC CCAGGACACT 
GATGACCTCA CTGTCTTCAA CCACCACGTT GACCTCTGCC TGAACCAGGA GGTCCTACAT 
GAGCTGGGGG GACAGACATC ATCTCGCATA AATACACCTC CAGTTACAAA TAGCAAGGCC 
ATAGATAGAG GCCATCTGCT GCCAAAGCAA ACAAGTAAAG GCAAAAGCAA AAGAAAGGAC 
CCGCCTTCCT CTCCTCCTTC GAAGAGAGCT AAAGGCTTCG GTCCTCACAA CACTATTGAC 
AGGTTCTTCA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022611330.1
CDS	117..2771
Translation	MENGIASTKGEGFLSRMALNDNKAGMEGLDRDKINKIIMESSKGSRFYENELKREQQVNQRIEKMMLQKAQITEQQLKKAQAQVEKVASALERSRDLSRVIVHVDMDAFYAAVEMRDCPELKDKPMAVGSMSMLSTSNYHARKYGVRAAMPGFIAKKLCPNLVIVPTNFDKYRAVSDEIREIFADYDPHFQPLSLDEAYLDFTDHLDYRQSWPESSRTHHFRASSTATGEEQSELPQEALPEVKDLSPVLFEDSPSTSPCLLGSGGGGGAFEVFGTSVEEAVREMRFRIEQKTMLTASAGIAPNMMLAKVCSDKNKPNGQYRLPSTREAVMDFVHNLPVRKVSGIGKVSEKMLNALGISSCSHLGQQMALLSLLFSETAWHHFMEVSLGLSSTYIPRHEDRKSMSTERTFKELSKAEEQFSLCRELCEDLAEDMKKEDLKGKTVTLKLKNVNFEVKTRALTLLCAVATVDEIFAVAKDLLKTEIENESPQPLRLRLMGVRISAFVSSDDKKPLQRSIIGFLQPGKTDSSGSIQGMHQNPEKERLTNHSLQTWALTCPPPVQKCQKQEEVPWRSKQKGLEGGQTGEEPQQSFFQRAHAKRLQLQAARSSTQEEGHGENVSVINSIDSDAQKGVESSSKAHKSTASDLPENDFVEAQASTSGCGGTASESLTCPVCFKQVETTDLNVFNRHIDQCLSESSRKPNRSTDSDSESDLDLDIVRNGCKVLEEWREECEVNEEKNSEELESNRVDPQWQHGLKNDPTQTVLLINGDKETVTSQQPQSCNDKGPVFICPVCQLTQDTDDLTVFNHHVDLCLNQEVLHELGGQTSSRINTPPVTNSKAIDRGHLLPKQTSKGKSKRKDPPSSPPSKRAKGFGPHNTIDRFFR

Target ORF information:

RefSeq Version	XM_022755609.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili DNA polymerase kappa (polk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022755609.1

ORF Insert Sequence:

ATGGAAAATG GAATTGCATC CACTAAAGGA GAGGGCTTCC TCTCCAGAAT GGCACTTAAT 
GACAACAAGG CCGGTATGGA GGGTCTTGAC AGGGACAAGA TTAACAAGAT CATCATGGAG 
TCATCGAAGG GATCAAGGTT TTACGAGAAT GAGCTGAAGA GAGAGCAGCA GGTGAACCAG 
CGCATTGAGA AAATGATGCT ACAAAAGGCA CAGATCACAG AGCAGCAGTT AAAGAAAGCA 
CAAGCTCAGG TGGAGAAGGT CGCCTCAGCG CTGGAGAGGA GTCGTGATCT AAGCCGTGTG 
ATTGTGCATG TGGACATGGA TGCTTTCTAC GCTGCTGTGG AGATGAGAGA CTGTCCTGAG 
CTGAAGGACA AGCCCATGGC TGTAGGATCC ATGAGCATGC TGTCAACATC TAACTACCAT 
GCCAGGAAGT ACGGGGTCCG AGCCGCCATG CCTGGGTTCA TTGCAAAGAA ACTCTGCCCC 
AACTTAGTCA TCGTTCCAAC TAACTTTGAT AAATACAGAG CTGTGAGTGA TGAGATCCGG 
GAAATTTTTG CAGACTATGA CCCTCACTTC CAGCCATTGA GCCTGGATGA AGCCTATCTG 
GATTTTACAG ACCACCTGGA CTACAGGCAG AGCTGGCCGG AGTCCTCTCG TACACATCAC 
TTCCGCGCCA GCAGCACCGC TACAGGTGAA GAGCAGAGTG AACTTCCCCA GGAGGCACTG 
CCAGAGGTGA AAGACCTGTC TCCTGTCCTG TTTGAGGACA GCCCGAGCAC CTCTCCCTGC 
TTGCTGGGCT CTGGTGGTGG TGGTGGTGCA TTCGAGGTGT TTGGGACGTC TGTGGAGGAG 
GCTGTGAGAG AGATGCGCTT CCGCATTGAG CAGAAGACCA TGCTGACTGC CAGTGCAGGA 
ATTGCTCCAA ACATGATGCT TGCCAAGGTG TGCAGTGACA AGAACAAGCC CAACGGCCAG 
TACAGACTCC CCTCCACCAG AGAGGCAGTC ATGGACTTCG TCCATAATCT TCCAGTTCGC 
AAAGTTTCTG GCATTGGGAA AGTGAGTGAG AAGATGCTCA ATGCTTTGGG AATCAGTAGC 
TGTTCTCACC TTGGCCAGCA GATGGCACTG CTGTCACTGT TGTTCTCAGA GACAGCCTGG 
CATCATTTCA TGGAGGTTTC CCTGGGTCTG AGCTCCACAT ATATACCGAG GCATGAGGAC 
AGAAAAAGCA TGAGCACGGA AAGGACATTT AAAGAACTGA GCAAAGCTGA GGAACAGTTT 
TCTTTATGCA GGGAGCTCTG TGAAGATTTA GCAGAAGACA TGAAGAAAGA AGATCTGAAG 
GGTAAAACCG TAACACTGAA GCTTAAGAAC GTGAACTTTG AGGTGAAAAC CAGGGCGCTG 
ACGCTGCTGT GTGCTGTTGC TACAGTGGAT GAGATCTTTG CTGTTGCTAA GGACCTACTG 
AAAACGGAAA TAGAAAATGA AAGCCCCCAG CCACTCAGAC TGAGGCTCAT GGGTGTTCGG 
ATTTCTGCTT TTGTCAGTTC GGATGATAAA AAGCCCCTGC AGAGGAGCAT CATTGGCTTT 
CTTCAGCCAG GAAAGACAGA CTCCAGTGGC TCTATCCAAG GAATGCACCA AAACCCTGAG 
AAAGAGCGTC TCACCAATCA CTCCCTCCAA ACCTGGGCCC TTACTTGTCC TCCCCCAGTC 
CAGAAGTGCC AGAAACAAGA GGAAGTTCCC TGGCGGAGCA AGCAGAAGGG GTTAGAGGGA 
GGTCAGACTG GTGAGGAACC CCAACAGTCT TTCTTCCAAA GGGCCCATGC CAAAAGATTG 
CAACTCCAGG CAGCGAGGTC AAGCACACAA GAGGAAGGAC ACGGGGAGAA TGTTTCTGTC 
ATTAATTCCA TAGATTCTGA TGCTCAAAAG GGTGTAGAGT CATCATCAAA AGCGCACAAG 
AGTACAGCCT CAGATCTCCC AGAAAACGAC TTTGTTGAGG CCCAGGCATC CACATCAGGC 
TGTGGTGGCA CAGCGTCAGA GAGCCTCACC TGTCCCGTGT GTTTCAAGCA GGTGGAGACG 
ACTGATTTGA ATGTCTTCAA CAGACATATA GACCAGTGTC TCAGCGAATC TTCACGAAAA 
CCAAATCGAA GCACAGATTC GGACTCAGAG TCAGATTTGG ACTTAGACAT TGTTCGTAAC 
GGTTGTAAGG TGCTGGAAGA ATGGAGGGAG GAGTGTGAGG TTAATGAGGA GAAGAACAGT 
GAAGAGTTGG AGTCGAACAG AGTCGATCCT CAGTGGCAAC ACGGCTTGAA AAACGATCCC 
ACTCAAACGG TTTTGTTGAT CAATGGTGAC AAGGAAACCG TGACCTCGCA ACAGCCTCAG 
TCATGTAATG ATAAAGGCCC TGTCTTCATC TGCCCAGTGT GTCAGCTGAC CCAGGACACT 
GATGACCTCA CTGTCTTCAA CCACCACGTT GACCTCTGCC TGAACCAGGA GGTCCTACAT 
GAGCTGGGGG GACAGACATC ATCTCGCATA AATACACCTC CAGTTACAAA TAGCAAGGCC 
ATAGATAGAG GCCATCTGCT GCCAAAGCAA ACAAGTAAAG GCAAAAGCAA AAGAAAGGAC 
CCGCCTTCCT CTCCTCCTTC GAAGAGAGCT AAAGGCTTCG GTCCTCACAA CACTATTGAC 
AGGTTCTTCA GGTGA