pif1 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	pif1
Entrez Gene ID	111228881
Full Name	PIF1 5'-to-3' DNA helicase
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022754990.1	XP_022610711.1	ATP-dependent DNA helicase PIF1

Pathways from BioSystems
Gene Ontology

The following pif1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pif1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe201552	XM_022754990.1 Latest version!	Seriola dumerili PIF1 5'-to-3' DNA helicase (pif1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pif1 ( XM_022754990.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022610711.1 Seriola dumerili PIF1 5'-to-3' DNA helicase (pif1), mRNA.

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CloneID	OSe201552
Clone ID Related Accession (Same CDS sequence)	XM_022754990.1
Accession Version	XM_022754990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	ATP-dependent DNA helicase PIF1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174481.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGCCG GAGAGGACGG AGCCCAGCTG CAGTGCTGTG TGGCGATGGA GCAGCTCAAC 
GCCTCCGGCC AGGCCACCCG GAGGCAGGTC ATCCGGAAAG CCTCCGTTAT TCTGGGCCGG 
AATGAGTTCC AGGAGATTAT CCTCCGGGTC CACGACGGGA AAGTCCCGCA GAGTTACCAT 
CTGAAAGAAT TCAAACTTTT CACCAAGTTC GCCAGAGATG GTAAATGCAC CGTCAAACTG 
CTCCCTGAAA ACATCCAGGT GCTCATCTCC AATTGCCCCC CTGACCAGCT GAGCCTCTTC 
CTCAAAACCC TGAGCATCAA ACACCAGGCC TGGCGGAGCA GCAAGCCCCT GAGCGACAGA 
GAGAAGCTCA AGGCCGGTCT TCCCCGCAGC TTCGAGGCCA TCAGCCCCCT GCAGCAAAAG 
GACATCCAGA AGGTCAATGA GCTGAGAAGT AAAGCGGCTC CCAAAGGACT GACAGACCGC 
ACCAACAACA CGACCGCGGC AAGAACAGGA CAGCAAGTCA AGAGGTCAAG GAGTGACAGC 
AACTTTAGCC CAGTGAAGAC AAACCCGACT AAGAAGCCCA TCCTGTCTCT GCCATCTCGT 
AAACTCAATA AAGAACAGGC TGCTGTTCTC AGTGCGGTGC TCAGCGGCAA GAACGTCTTC 
TTCACTGGAA GTGCCGGAAC AGGAAAGTCT TTCTTACTCA AGAGAATCAT GGGATCGCTT 
CCTCCAAAGA GCACCTTTGC TACAGCCAGT ACAGGAGTGG CTGCATGTCA CATTGGAGGA 
ACAACATTAC ACAGTTTTGC AGGTGTTGGC TCGGGCTCTG CCCCGCTGGA GCAGTGTATT 
GAGCTGGCTC AGAGGCCCGG GGTGCTGCAG CACTGGACCA GCTGTCGACA CCTCATCATC 
GATGAGATCT CCATGGTGGA GGCCCAGTTC TTTGACAAGC TTGAGTCCGT TGCAAGGTCG 
GTGAGGAGGT CCACTGAGCC GTTTGGAGGC ATCCAGCTGA TCGTGTGTGG CGACTTCCTT 
CAGCTGCCTC CGGTTTCTAA AGGGAAGGAG AAGGCTAGCT TCTGCTTCCA GGCAAGGAGT 
TGGCGTAAGG TCATCCAGGT CAACATGGAG CTAACAGAAG TACGAAGGCA AACGGACCAG 
TCGTTCATCT CCCTGCTGCA GGCAGTGAGG GTGGGCAGGG TGACGGAGGA AGTTACGGCT 
AAGCTAATGG AAAGCGCCTA CCATCACATC GAGAGGGACG GTATTCTGGC AACCAGGCTG 
TGCACACACA AGGATGACGT GGAGCTCACA AACGAGAGCA AACTCCAGCA GCTGCCAGGG 
TCAGTGCGTG TGTTTGAGGC ACTCGACAGC GACCTGGCCC TGGTGAAGAC TATAGACGCT 
CACAGCCCCG TCAGCAGGCT GATCCAACTC AAAGTGGGAG CTCAGGTCAT GCTGACAAAG 
AACCTGGATG TTGCTCGAGG TCTGGTGAAT GGAGCACGAG GGGTTGTTGT AGCTTTTGAT 
TCTGGAAAAC ATGGACTCCC ACGTGTGCGT TTCCTGTGCG GCGTTACAGA GGTGCTGAAG 
ACAGAGCGCT GGGTGTTCAA GTCCGGTGGT GGGATCCATC TGAGTCGACA GCAGTTGCCG 
CTCAAACTTG CCTGGGCCAT CTCCATCCAC AAGAGCCAGG GAATGACACT GGACTGCGTG 
GAGATCTCTC TGGCACGTGT CTTTGAGAGC GGACAGGCCT ACGTAGCCTT GTCTCGAGCT 
CGGAGCCTGG AGGGTCTGAG GGTCATGGAC TTTGACCCCC GTGTGGTGCG TTCGGATCCA 
GATGTTCTCC TCTTCTATAA GAAACTGAGG AAGGAGAGGC TGCTCATGCA GGCTTCCATG 
GACGATTTTG TTTGCAACAA AGAAAACTCT TGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022610711.1
CDS	19..1914
Translation	MFAGEDGAQLQCCVAMEQLNASGQATRRQVIRKASVILGRNEFQEIILRVHDGKVPQSYHLKEFKLFTKFARDGKCTVKLLPENIQVLISNCPPDQLSLFLKTLSIKHQAWRSSKPLSDREKLKAGLPRSFEAISPLQQKDIQKVNELRSKAAPKGLTDRTNNTTAARTGQQVKRSRSDSNFSPVKTNPTKKPILSLPSRKLNKEQAAVLSAVLSGKNVFFTGSAGTGKSFLLKRIMGSLPPKSTFATASTGVAACHIGGTTLHSFAGVGSGSAPLEQCIELAQRPGVLQHWTSCRHLIIDEISMVEAQFFDKLESVARSVRRSTEPFGGIQLIVCGDFLQLPPVSKGKEKASFCFQARSWRKVIQVNMELTEVRRQTDQSFISLLQAVRVGRVTEEVTAKLMESAYHHIERDGILATRLCTHKDDVELTNESKLQQLPGSVRVFEALDSDLALVKTIDAHSPVSRLIQLKVGAQVMLTKNLDVARGLVNGARGVVVAFDSGKHGLPRVRFLCGVTEVLKTERWVFKSGGGIHLSRQQLPLKLAWAISIHKSQGMTLDCVEISLARVFESGQAYVALSRARSLEGLRVMDFDPRVVRSDPDVLLFYKKLRKERLLMQASMDDFVCNKENSW

Target ORF information:

RefSeq Version	XM_022754990.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili PIF1 5'-to-3' DNA helicase (pif1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022754990.1

ORF Insert Sequence:

ATGTTCGCCG GAGAGGACGG AGCCCAGCTG CAGTGCTGTG TGGCGATGGA GCAGCTCAAC 
GCCTCCGGCC AGGCCACCCG GAGGCAGGTC ATCCGGAAAG CCTCCGTTAT TCTGGGCCGG 
AATGAGTTCC AGGAGATTAT CCTCCGGGTC CACGACGGGA AAGTCCCGCA GAGTTACCAT 
CTGAAAGAAT TCAAACTTTT CACCAAGTTC GCCAGAGATG GTAAATGCAC CGTCAAACTG 
CTCCCTGAAA ACATCCAGGT GCTCATCTCC AATTGCCCCC CTGACCAGCT GAGCCTCTTC 
CTCAAAACCC TGAGCATCAA ACACCAGGCC TGGCGGAGCA GCAAGCCCCT GAGCGACAGA 
GAGAAGCTCA AGGCCGGTCT TCCCCGCAGC TTCGAGGCCA TCAGCCCCCT GCAGCAAAAG 
GACATCCAGA AGGTCAATGA GCTGAGAAGT AAAGCGGCTC CCAAAGGACT GACAGACCGC 
ACCAACAACA CGACCGCGGC AAGAACAGGA CAGCAAGTCA AGAGGTCAAG GAGTGACAGC 
AACTTTAGCC CAGTGAAGAC AAACCCGACT AAGAAGCCCA TCCTGTCTCT GCCATCTCGT 
AAACTCAATA AAGAACAGGC TGCTGTTCTC AGTGCGGTGC TCAGCGGCAA GAACGTCTTC 
TTCACTGGAA GTGCCGGAAC AGGAAAGTCT TTCTTACTCA AGAGAATCAT GGGATCGCTT 
CCTCCAAAGA GCACCTTTGC TACAGCCAGT ACAGGAGTGG CTGCATGTCA CATTGGAGGA 
ACAACATTAC ACAGTTTTGC AGGTGTTGGC TCGGGCTCTG CCCCGCTGGA GCAGTGTATT 
GAGCTGGCTC AGAGGCCCGG GGTGCTGCAG CACTGGACCA GCTGTCGACA CCTCATCATC 
GATGAGATCT CCATGGTGGA GGCCCAGTTC TTTGACAAGC TTGAGTCCGT TGCAAGGTCG 
GTGAGGAGGT CCACTGAGCC GTTTGGAGGC ATCCAGCTGA TCGTGTGTGG CGACTTCCTT 
CAGCTGCCTC CGGTTTCTAA AGGGAAGGAG AAGGCTAGCT TCTGCTTCCA GGCAAGGAGT 
TGGCGTAAGG TCATCCAGGT CAACATGGAG CTAACAGAAG TACGAAGGCA AACGGACCAG 
TCGTTCATCT CCCTGCTGCA GGCAGTGAGG GTGGGCAGGG TGACGGAGGA AGTTACGGCT 
AAGCTAATGG AAAGCGCCTA CCATCACATC GAGAGGGACG GTATTCTGGC AACCAGGCTG 
TGCACACACA AGGATGACGT GGAGCTCACA AACGAGAGCA AACTCCAGCA GCTGCCAGGG 
TCAGTGCGTG TGTTTGAGGC ACTCGACAGC GACCTGGCCC TGGTGAAGAC TATAGACGCT 
CACAGCCCCG TCAGCAGGCT GATCCAACTC AAAGTGGGAG CTCAGGTCAT GCTGACAAAG 
AACCTGGATG TTGCTCGAGG TCTGGTGAAT GGAGCACGAG GGGTTGTTGT AGCTTTTGAT 
TCTGGAAAAC ATGGACTCCC ACGTGTGCGT TTCCTGTGCG GCGTTACAGA GGTGCTGAAG 
ACAGAGCGCT GGGTGTTCAA GTCCGGTGGT GGGATCCATC TGAGTCGACA GCAGTTGCCG 
CTCAAACTTG CCTGGGCCAT CTCCATCCAC AAGAGCCAGG GAATGACACT GGACTGCGTG 
GAGATCTCTC TGGCACGTGT CTTTGAGAGC GGACAGGCCT ACGTAGCCTT GTCTCGAGCT 
CGGAGCCTGG AGGGTCTGAG GGTCATGGAC TTTGACCCCC GTGTGGTGCG TTCGGATCCA 
GATGTTCTCC TCTTCTATAA GAAACTGAGG AAGGAGAGGC TGCTCATGCA GGCTTCCATG 
GACGATTTTG TTTGCAACAA AGAAAACTCT TGGTGA