efnb1 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	efnb1
Entrez Gene ID	111226623
Full Name	ephrin B1
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022751874.1	XP_022607595.1	ephrin-B1

Pathways from BioSystems
Gene Ontology

The following efnb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the efnb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe199031	XM_022751874.1 Latest version!	Seriola dumerili ephrin B1 (efnb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

efnb1 ( XM_022751874.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022607595.1 Seriola dumerili ephrin B1 (efnb1), mRNA.

Price & Availability↑

CloneID	OSe199031
Clone ID Related Accession (Same CDS sequence)	XM_022751874.1
Accession Version	XM_022751874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	ephrin-B1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174416.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGAC TCGGTTGTTG GAAGTATTAC CTCTGGATCT TTATTTTAAT GTGCAGGTCG 
GCGTTACCTT CGGCGAAATC GCTGGAGACT ATAATTTGGA GCTCGCAGAA TCCCAAGTTT 
CTGGCTGGAA AAGGCCTGGT GATCTACCCA GACATCGGGG ACAAGCTGGA CATCATCTGT 
CCCAAAGCGG ACCAAGGCAG AGAATATGAG TTCTACAAGT TGTACCTGGT GAAGAGGGAG 
CAGGCGGAGA GCTGCAGTAC CGTCCTCGAT CCTAATGTCC TGGTCAACTG CAACAAGCCC 
GAGAAGGACA TCAAGTTCAC CATCAAGTTC CAGGAGTTCA GCCCCAACTA CATGGGCCTG 
GAGTTCAAGA AGAACGTCAA CTACTACATC ACCTCAACCT CCAACGGCAC AGTCGAGGGC 
CTGGAGAACC GAGAGGGAGG CGTGTGCAAG AGCCGAGCCA TGAAAGTCAT CATGAAAGTC 
GGGCAAGTTC CGAATGCAGT GAACCCTGTG GCGCCGGAGG CTCCGGAAGA CAATGCCATA 
CCGGAGTCCA GTCCCAGTCC GCCTGACCAG GGTCGGATTG GACCCGAGAT ACCAGGAAAC 
CCTGACCACA TGAATCAGGA CCAGGGTTCC TCGGCAAACT CGGACGGCTT CCTCGGATCC 
AGAGTGGCCA TGTTCTCCGT CATTGGGGCC GGCTGCATCA TCTTCCTCCT GCTCATCCTC 
CTCCTCGTCA TCCTGCTCAT AAAGATGCGC AAGCGCGCCA GGAAGAACGC CCACTCGCAG 
CCCCGCCCCT CGGCACTGTC GCTCAGCACG CTGTCCAATC CCAAACTGCC CGGGGGCACG 
GCTGGCACGG AGCCCAGTGA CATCATCATT CCACTGCGGA CAGAGAACAA CTACTGCCCT 
CACTATGAGA AGGTGAGCGG CGACTATGGC CACCCCGTCT ACATTGTCCA GGAGATGCCC 
CCGCAGAGCC CTGCCAACAT CTACTACAAA GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022607595.1
CDS	896..1891
Translation	MAGLGCWKYYLWIFILMCRSALPSAKSLETIIWSSQNPKFLAGKGLVIYPDIGDKLDIICPKADQGREYEFYKLYLVKREQAESCSTVLDPNVLVNCNKPEKDIKFTIKFQEFSPNYMGLEFKKNVNYYITSTSNGTVEGLENREGGVCKSRAMKVIMKVGQVPNAVNPVAPEAPEDNAIPESSPSPPDQGRIGPEIPGNPDHMNQDQGSSANSDGFLGSRVAMFSVIGAGCIIFLLLILLLVILLIKMRKRARKNAHSQPRPSALSLSTLSNPKLPGGTAGTEPSDIIIPLRTENNYCPHYEKVSGDYGHPVYIVQEMPPQSPANIYYKV

Target ORF information:

RefSeq Version	XM_022751874.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili ephrin B1 (efnb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022751874.1

ORF Insert Sequence:

ATGGCGGGAC TCGGTTGTTG GAAGTATTAC CTCTGGATCT TTATTTTAAT GTGCAGGTCG 
GCGTTACCTT CGGCGAAATC GCTGGAGACT ATAATTTGGA GCTCGCAGAA TCCCAAGTTT 
CTGGCTGGAA AAGGCCTGGT GATCTACCCA GACATCGGGG ACAAGCTGGA CATCATCTGT 
CCCAAAGCGG ACCAAGGCAG AGAATATGAG TTCTACAAGT TGTACCTGGT GAAGAGGGAG 
CAGGCGGAGA GCTGCAGTAC CGTCCTCGAT CCTAATGTCC TGGTCAACTG CAACAAGCCC 
GAGAAGGACA TCAAGTTCAC CATCAAGTTC CAGGAGTTCA GCCCCAACTA CATGGGCCTG 
GAGTTCAAGA AGAACGTCAA CTACTACATC ACCTCAACCT CCAACGGCAC AGTCGAGGGC 
CTGGAGAACC GAGAGGGAGG CGTGTGCAAG AGCCGAGCCA TGAAAGTCAT CATGAAAGTC 
GGGCAAGTTC CGAATGCAGT GAACCCTGTG GCGCCGGAGG CTCCGGAAGA CAATGCCATA 
CCGGAGTCCA GTCCCAGTCC GCCTGACCAG GGTCGGATTG GACCCGAGAT ACCAGGAAAC 
CCTGACCACA TGAATCAGGA CCAGGGTTCC TCGGCAAACT CGGACGGCTT CCTCGGATCC 
AGAGTGGCCA TGTTCTCCGT CATTGGGGCC GGCTGCATCA TCTTCCTCCT GCTCATCCTC 
CTCCTCGTCA TCCTGCTCAT AAAGATGCGC AAGCGCGCCA GGAAGAACGC CCACTCGCAG 
CCCCGCCCCT CGGCACTGTC GCTCAGCACG CTGTCCAATC CCAAACTGCC CGGGGGCACG 
GCTGGCACGG AGCCCAGTGA CATCATCATT CCACTGCGGA CAGAGAACAA CTACTGCCCT 
CACTATGAGA AGGTGAGCGG CGACTATGGC CACCCCGTCT ACATTGTCCA GGAGATGCCC 
CCGCAGAGCC CTGCCAACAT CTACTACAAA GTCTGA