trim62 cDNA ORF clone, Seriola dumerili(greater amberjack)

Gene
Clones
gRNAs

Summary

Gene Symbol	trim62
Entrez Gene ID	111224009
Full Name	tripartite motif containing 62
Gene Type	protein-coding
Organism	Seriola dumerili(greater amberjack)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022748355.1	XP_022604076.1	E3 ubiquitin-protein ligase TRIM62

Pathways from BioSystems
Gene Ontology

The following trim62 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trim62 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSe217918	XM_022748355.1 Latest version!	Seriola dumerili tripartite motif containing 62 (trim62), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trim62 ( XM_022748355.1 ) cDNA ORF clone, Seriola dumerili(greater amberjack) -> XP_022604076.1 Seriola dumerili tripartite motif containing 62 (trim62), mRNA.

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CloneID	OSe217918
Clone ID Related Accession (Same CDS sequence)	XM_022748355.1
Accession Version	XM_022748355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1428bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Seriola dumerili(greater amberjack)
Product	E3 ubiquitin-protein ligase TRIM62
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019174376.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Seriola dumerili Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGTT GTCTGAAGGA CGAGCTCCTC TGTTCCATCT GCCTCAGCAT CTACCAGGAC 
CCGGTCAGCT TTGGCTGCGA GCACTACTTC TGCAGAAAGT GCATTACCGA GCACTGGAGC 
AGACAGGAGC CTCACGGAAC ACGAGACTGC CCAGAGTGCC GGAGGACCTT CACCGATCCT 
CTCCTCTCCC CGAGTCTCAA GTTGTCCAAC ATTGTGGAGC GCTACTCGGC CTTCCCGCTG 
GACGCCATCC TTAACGCTCA GAGGAGCTCC TATCCCTGCA AGGACCACGA GAAGGTCAAG 
CTCTTCTGCC TCACTGATAA AAGTCTGGTG TGCTTCTTCT GCGACGAGCC AGCACTACAC 
GAGCAGCACC AAGTGACCAC TATTGACGAG GCTTACGAGG AGATACAGAG GGAGATGAAG 
GAGCAGCTGG CCACCCTGCA GGACAGTGAG AAGGGACACA CCGAGGCCCT GCAGCTCCTG 
CAGAGACAAC TCACTGAGAC CAAGTCCTCG GCCAAAAGTC TGCGCGCAAC CATCGGAGAT 
GCATTCGAGC GCCTTCACCG TTTCCTGCGC GAGCGTCAGA AGAGCATGCT GGAAGAGTTA 
GAGATGGATA CGGCCCGCAA GCTCGCCGAC ATCGAGCACA AGATCCAGCG CTACAGTCAG 
CAGCTGCGCG ACGTCAAGGA GGGCATCCAG ATCCTGCAGG AGCGCCTCAA TGAGACAGGA 
CGACACGACT TCCTGGAGGG AGTAGCCGTC ACGTCTGAGA GGATTAAAGG GAAGATACAT 
GAGACCAATC TGACGTATGA AGACTTCCCA ACATCCAAGT ACATGGGGCC CTTACAGTAC 
ACAATATGGA AGTCGCTCTT CCAGGATATT TCACCAGTGC CAGCAGCGTT GACCCTCGAC 
CCCATCACAG CCCACCAAAG ACTGATCCTT TCAGACGACT GTACCATCGT GGCCTACGGG 
AATCTCCATC CGCAGCCGCT GCAGGACTCC CCGCGCCGCT TTGATGTGGA GGTGTCTGTT 
CTGGGCGCAG AGGGCTTCGC CTCAGGTGTC CATTACTGGG AAGTGATGGT GTCGGAGAAG 
ACCCAGTGGA TGATCGGTGT GGCCAACGAG ACAGTCAGTC GCAAGGGCAG CATCCAGATC 
CAGCCCAGTC GCGGCTTCTA CTGCATCGTC ATGCACGACG GGAACCAGTA CAGTGCCTGC 
ACCGAGCCCT GGACCCGTCT CAACGTCAAG AGCAAGCTGG AGAAGGTGGG GGTGTATCTG 
GACTACCCGA AAGGCCTGCT CATCTTCTAC AACGCGGACG ACATGTCCTG GCTCTACACC 
TACCGAGAGA AATTCCCCGC CAAGGTCTTC CCCTATTTCA GCCCCGGCCA GAGCCATGCT 
AACGGCAAGA ACGTGCAGCC ATTGCGAATC AACACTGTAC GCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022604076.1
CDS	130..1557
Translation	MACCLKDELLCSICLSIYQDPVSFGCEHYFCRKCITEHWSRQEPHGTRDCPECRRTFTDPLLSPSLKLSNIVERYSAFPLDAILNAQRSSYPCKDHEKVKLFCLTDKSLVCFFCDEPALHEQHQVTTIDEAYEEIQREMKEQLATLQDSEKGHTEALQLLQRQLTETKSSAKSLRATIGDAFERLHRFLRERQKSMLEELEMDTARKLADIEHKIQRYSQQLRDVKEGIQILQERLNETGRHDFLEGVAVTSERIKGKIHETNLTYEDFPTSKYMGPLQYTIWKSLFQDISPVPAALTLDPITAHQRLILSDDCTIVAYGNLHPQPLQDSPRRFDVEVSVLGAEGFASGVHYWEVMVSEKTQWMIGVANETVSRKGSIQIQPSRGFYCIVMHDGNQYSACTEPWTRLNVKSKLEKVGVYLDYPKGLLIFYNADDMSWLYTYREKFPAKVFPYFSPGQSHANGKNVQPLRINTVRL

Target ORF information:

RefSeq Version	XM_022748355.1
Organism	Seriola dumerili(greater amberjack)
Definition	Seriola dumerili tripartite motif containing 62 (trim62), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022748355.1

ORF Insert Sequence:

ATGGCTTGTT GTCTGAAGGA CGAGCTCCTC TGTTCCATCT GCCTCAGCAT CTACCAGGAC 
CCGGTCAGCT TTGGCTGCGA GCACTACTTC TGCAGAAAGT GCATTACCGA GCACTGGAGC 
AGACAGGAGC CTCACGGAAC ACGAGACTGC CCAGAGTGCC GGAGGACCTT CACCGATCCT 
CTCCTCTCCC CGAGTCTCAA GTTGTCCAAC ATTGTGGAGC GCTACTCGGC CTTCCCGCTG 
GACGCCATCC TTAACGCTCA GAGGAGCTCC TATCCCTGCA AGGACCACGA GAAGGTCAAG 
CTCTTCTGCC TCACTGATAA AAGTCTGGTG TGCTTCTTCT GCGACGAGCC AGCACTACAC 
GAGCAGCACC AAGTGACCAC TATTGACGAG GCTTACGAGG AGATACAGAG GGAGATGAAG 
GAGCAGCTGG CCACCCTGCA GGACAGTGAG AAGGGACACA CCGAGGCCCT GCAGCTCCTG 
CAGAGACAAC TCACTGAGAC CAAGTCCTCG GCCAAAAGTC TGCGCGCAAC CATCGGAGAT 
GCATTCGAGC GCCTTCACCG TTTCCTGCGC GAGCGTCAGA AGAGCATGCT GGAAGAGTTA 
GAGATGGATA CGGCCCGCAA GCTCGCCGAC ATCGAGCACA AGATCCAGCG CTACAGTCAG 
CAGCTGCGCG ACGTCAAGGA GGGCATCCAG ATCCTGCAGG AGCGCCTCAA TGAGACAGGA 
CGACACGACT TCCTGGAGGG AGTAGCCGTC ACGTCTGAGA GGATTAAAGG GAAGATACAT 
GAGACCAATC TGACGTATGA AGACTTCCCA ACATCCAAGT ACATGGGGCC CTTACAGTAC 
ACAATATGGA AGTCGCTCTT CCAGGATATT TCACCAGTGC CAGCAGCGTT GACCCTCGAC 
CCCATCACAG CCCACCAAAG ACTGATCCTT TCAGACGACT GTACCATCGT GGCCTACGGG 
AATCTCCATC CGCAGCCGCT GCAGGACTCC CCGCGCCGCT TTGATGTGGA GGTGTCTGTT 
CTGGGCGCAG AGGGCTTCGC CTCAGGTGTC CATTACTGGG AAGTGATGGT GTCGGAGAAG 
ACCCAGTGGA TGATCGGTGT GGCCAACGAG ACAGTCAGTC GCAAGGGCAG CATCCAGATC 
CAGCCCAGTC GCGGCTTCTA CTGCATCGTC ATGCACGACG GGAACCAGTA CAGTGCCTGC 
ACCGAGCCCT GGACCCGTCT CAACGTCAAG AGCAAGCTGG AGAAGGTGGG GGTGTATCTG 
GACTACCCGA AAGGCCTGCT CATCTTCTAC AACGCGGACG ACATGTCCTG GCTCTACACC 
TACCGAGAGA AATTCCCCGC CAAGGTCTTC CCCTATTTCA GCCCCGGCCA GAGCCATGCT 
AACGGCAAGA ACGTGCAGCC ATTGCGAATC AACACTGTAC GCCTTTAA