Atp5j cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Atp5j
Entrez Gene ID	94271
Full Name	ATP synthase peripheral stalk subunit F6
Synonyms	Atp5j
General protein information	Preferred Names ATP synthase peripheral stalk subunit F6 Names ATP synthase-coupling factor 6, mitochondrial ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F6 ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 ATPase subunit F6 coupling factor 6
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 11 Map Location: 11q11
Summary	coupling factor 6 of the mitochondrial ATP synthase complex [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017598086.1	XP_017453575.1	ATP synthase-coupling factor 6, mitochondrial isoform X1
XM_008768602.2	XP_008766824.1	ATP synthase-coupling factor 6, mitochondrial isoform X1
XM_017598085.1	XP_017453574.1	ATP synthase-coupling factor 6, mitochondrial isoform X1
NM_053602.2	NP_446054.1	ATP synthase-coupling factor 6, mitochondrial precursor
XM_008768603.1	XP_008766825.1	ATP synthase-coupling factor 6, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ATP5J	NP_777142.1
Gallus gallus (chicken)	ATP5J	XP_001231639.1
Danio rerio (zebrafish)	atp5j	NP_998472.1
Homo sapiens (human)	ATP5J	NP_001003701.1
Pan troglodytes (chimpanzee)	ATP5J	XP_001158570.1
	ATP5J	XP_001105213.2
Xenopus tropicalis (tropical clawed frog)	atp5j	NP_001017042.1
Mus musculus (house mouse)	Atp5j	NP_058035.1
Canis lupus familiaris (dog)	ATP5J	XP_003434019.1
Rattus norvegicus (Norway rat)	Atp5j	NP_446054.1

Gene Ontology
Bibliography

Related articles in PubMed

Marine collagen peptides reduce endothelial cell injury in diabetic rats by inhibiting apoptosis and the expression of coupling factor 6 and microparticles.
Zhu C, Zhang W, Liu J, Mu B, Zhang F, Lai N, Zhou J, Xu A, Li Y
Molecular medicine reports16(4)3947-3957(2017 Oct)

Coupling Factor 6 Is Upregulated in Monocrotaline-induced Pulmonary Arterial Hypertension in Rats.
Li N, Yin J, Cai W, Liu J, Zhang N, Yan S, Song L, Li X
The American journal of the medical sciences352(6)631-636(2016 Dec)

Lung-specific RNA interference of coupling factor 6, a novel peptide, attenuates pulmonary arterial hypertension in rats.
Yin J, You S, Li N, Jiao S, Hu H, Xue M, Wang Y, Cheng W, Liu J, Xu M, Yan S, Li X
Respiratory research17(1)99(2016 Aug)

Mitochondrial coupling factor 6 upregulation in hypertension-induced cardiac hypertrophy.
He T, Guan A, Shi Y, Ge Z, Dai H
Herz40(5)783-7(2015 Aug)

Application of a discovery to targeted LC-MS proteomics approach to identify deregulated proteins associated with idiosyncratic liver toxicity in a rat model of LPS/diclofenac co-administration.
Ramm S, Morissey B, Hernandez B, Rooney C, Pennington SR, Mally A
Toxicology331100-11(2015 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

plasma level increased markedly during pathogenesis of pulmonary arterial hypertension (PAH), indicating that CF6 may be involved in the development of PAH
Title: Coupling Factor 6 Is Upregulated in Monocrotaline-induced Pulmonary Arterial Hypertension in Rats.

CF6 shRNA effectively inhibited CF6 expression, abolished lung macrophage infiltration, reversed endothelial dysfunction and vascular remodeling, and ameliorated the severity of pulmonary hypertension and right ventricular dysfunction at 4 weeks both as a pretreatment and rescue intervention
Title: Lung-specific RNA interference of coupling factor 6, a novel peptide, attenuates pulmonary arterial hypertension in rats.

CF6 protein was upregulated in cardiac hypertrophy induced by hypertension; further mechanisms involved in this process should be investigated.
Title: Mitochondrial coupling factor 6 upregulation in hypertension-induced cardiac hypertrophy.

suppresses prostacyclin generation in resistance arteriole vascular smooth muscle cells, which is enhanced in spontaneously hypertensive rats by the overproduction of CF6 and the hyperresponsiveness to CF6
Title: Coupling factor 6-induced prostacyclin inhibition is enhanced in vascular smooth muscle cells from spontaneously hypertensive rats.

The following Atp5j gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Atp5j cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12264	NM_053602.2 Latest version!	Rattus norvegicus ATP synthase peripheral stalk subunit F6 (Atp5pf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa12264	XM_008768603.1 Latest version!	Rattus norvegicus ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (Atp5j), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa12264	XM_008768602.2 Latest version!	Rattus norvegicus ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (Atp5j), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa12264	XM_017598086.1 Latest version!	Rattus norvegicus ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (Atp5j), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa12264	XM_017598085.1 Latest version!	Rattus norvegicus ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (Atp5j), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Atp5j ( NM_053602.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446054.1 Rattus norvegicus ATP synthase peripheral stalk subunit F6 (Atp5pf), mRNA.

Price & Availability↑

CloneID	ORa12264
Clone ID Related Accession (Same CDS sequence)	XM_017598086.1 , XM_008768602.2 , XM_017598085.1 , NM_053602.2 , XM_008768603.1
Accession Version	NM_053602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	ATP synthase-coupling factor 6, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X54510.1. On Feb 21, 2013 this sequence version replaced NM_053602.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X54510.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMN01906347 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGACTGTTC AGAGGATCTT CAGGCTCTCC TCTGTCCTTC GGTCAGCAGT CTCTGTGCAT 
TTGAGGAGGA ACATTGGTGT TACAGCTGTG GCGTTTAATA AGGAACTTGA TCCTGTACAG 
AAACTCTTCT TGGACAAGAT AAGAGAGTAC AAAGCAAAGC GACTGGCGTC TGGAGGACCT 
GTTGATACTG GCCCAGAATA TCAGCAAGAG GTGGACAGAG AGCTTTTTAA GCTTAAACAA 
ATGTATGGTA AAGGAGAGAT GGATAAGTTT CCTACCTTCA ATTTTGAGGA TCCCAAATTT 
GAAGTCCTCG ACAAACCCCA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446054.1
CDS	162..488
Translation	MTVQRIFRLSSVLRSAVSVHLRRNIGVTAVAFNKELDPVQKLFLDKIREYKAKRLASGGPVDTGPEYQQEVDRELFKLKQMYGKGEMDKFPTFNFEDPKFEVLDKPQS

Target ORF information:

RefSeq Version	NM_053602.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP synthase peripheral stalk subunit F6 (Atp5pf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053602.2

ORF Insert Sequence:

ATGACTGTTC AGAGGATCTT CAGGCTCTCC TCTGTCCTTC GGTCAGCAGT CTCTGTGCAT 
TTGAGGAGGA ACATTGGTGT TACAGCTGTG GCGTTTAATA AGGAACTTGA TCCTGTACAG 
AAACTCTTCT TGGACAAGAT AAGAGAGTAC AAAGCAAAGC GACTGGCGTC TGGAGGACCT 
GTTGATACTG GCCCAGAATA TCAGCAAGAG GTGGACAGAG AGCTTTTTAA GCTTAAACAA 
ATGTATGGTA AAGGAGAGAT GGATAAGTTT CCTACCTTCA ATTTTGAGGA TCCCAAATTT 
GAAGTCCTCG ACAAACCCCA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp5j ( XM_017598086.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453575.1 Rattus norvegicus ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (Atp5j), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa12264
Clone ID Related Accession (Same CDS sequence)	XM_017598086.1 , XM_008768602.2 , XM_017598085.1 , NM_053602.2 , XM_008768603.1
Accession Version	XM_017598086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP synthase-coupling factor 6, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTTC AGAGGATCTT CAGGCTCTCC TCTGTCCTTC GGTCAGCAGT CTCTGTGCAT 
TTGAGGAGGA ACATTGGTGT TACAGCTGTG GCGTTTAATA AGGAACTTGA TCCTGTACAG 
AAACTCTTCT TGGACAAGAT AAGAGAGTAC AAAGCAAAGC GACTGGCGTC TGGAGGACCT 
GTTGATACTG GCCCAGAATA TCAGCAAGAG GTGGACAGAG AGCTTTTTAA GCTTAAACAA 
ATGTATGGTA AAGGAGAGAT GGATAAGTTT CCTACCTTCA ATTTTGAGGA TCCCAAATTT 
GAAGTCCTCG ACAAACCCCA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453575.1
CDS	78..404
Translation	MTVQRIFRLSSVLRSAVSVHLRRNIGVTAVAFNKELDPVQKLFLDKIREYKAKRLASGGPVDTGPEYQQEVDRELFKLKQMYGKGEMDKFPTFNFEDPKFEVLDKPQS

Target ORF information:

RefSeq Version	XM_017598086.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (Atp5j), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598086.1

ORF Insert Sequence:

ATGACTGTTC AGAGGATCTT CAGGCTCTCC TCTGTCCTTC GGTCAGCAGT CTCTGTGCAT 
TTGAGGAGGA ACATTGGTGT TACAGCTGTG GCGTTTAATA AGGAACTTGA TCCTGTACAG 
AAACTCTTCT TGGACAAGAT AAGAGAGTAC AAAGCAAAGC GACTGGCGTC TGGAGGACCT 
GTTGATACTG GCCCAGAATA TCAGCAAGAG GTGGACAGAG AGCTTTTTAA GCTTAAACAA 
ATGTATGGTA AAGGAGAGAT GGATAAGTTT CCTACCTTCA ATTTTGAGGA TCCCAAATTT 
GAAGTCCTCG ACAAACCCCA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp5j ( XM_008768602.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766824.1 Rattus norvegicus ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (Atp5j), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa12264
Clone ID Related Accession (Same CDS sequence)	XM_017598086.1 , XM_008768602.2 , XM_017598085.1 , NM_053602.2 , XM_008768603.1
Accession Version	XM_008768602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP synthase-coupling factor 6, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008768602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTTC AGAGGATCTT CAGGCTCTCC TCTGTCCTTC GGTCAGCAGT CTCTGTGCAT 
TTGAGGAGGA ACATTGGTGT TACAGCTGTG GCGTTTAATA AGGAACTTGA TCCTGTACAG 
AAACTCTTCT TGGACAAGAT AAGAGAGTAC AAAGCAAAGC GACTGGCGTC TGGAGGACCT 
GTTGATACTG GCCCAGAATA TCAGCAAGAG GTGGACAGAG AGCTTTTTAA GCTTAAACAA 
ATGTATGGTA AAGGAGAGAT GGATAAGTTT CCTACCTTCA ATTTTGAGGA TCCCAAATTT 
GAAGTCCTCG ACAAACCCCA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766824.1
CDS	281..607
Translation	MTVQRIFRLSSVLRSAVSVHLRRNIGVTAVAFNKELDPVQKLFLDKIREYKAKRLASGGPVDTGPEYQQEVDRELFKLKQMYGKGEMDKFPTFNFEDPKFEVLDKPQS

Target ORF information:

RefSeq Version	XM_008768602.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (Atp5j), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768602.2

ORF Insert Sequence:

ATGACTGTTC AGAGGATCTT CAGGCTCTCC TCTGTCCTTC GGTCAGCAGT CTCTGTGCAT 
TTGAGGAGGA ACATTGGTGT TACAGCTGTG GCGTTTAATA AGGAACTTGA TCCTGTACAG 
AAACTCTTCT TGGACAAGAT AAGAGAGTAC AAAGCAAAGC GACTGGCGTC TGGAGGACCT 
GTTGATACTG GCCCAGAATA TCAGCAAGAG GTGGACAGAG AGCTTTTTAA GCTTAAACAA 
ATGTATGGTA AAGGAGAGAT GGATAAGTTT CCTACCTTCA ATTTTGAGGA TCCCAAATTT 
GAAGTCCTCG ACAAACCCCA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp5j ( XM_017598085.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453574.1 Rattus norvegicus ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (Atp5j), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa12264
Clone ID Related Accession (Same CDS sequence)	XM_017598086.1 , XM_008768602.2 , XM_017598085.1 , NM_053602.2 , XM_008768603.1
Accession Version	XM_017598085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP synthase-coupling factor 6, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTTC AGAGGATCTT CAGGCTCTCC TCTGTCCTTC GGTCAGCAGT CTCTGTGCAT 
TTGAGGAGGA ACATTGGTGT TACAGCTGTG GCGTTTAATA AGGAACTTGA TCCTGTACAG 
AAACTCTTCT TGGACAAGAT AAGAGAGTAC AAAGCAAAGC GACTGGCGTC TGGAGGACCT 
GTTGATACTG GCCCAGAATA TCAGCAAGAG GTGGACAGAG AGCTTTTTAA GCTTAAACAA 
ATGTATGGTA AAGGAGAGAT GGATAAGTTT CCTACCTTCA ATTTTGAGGA TCCCAAATTT 
GAAGTCCTCG ACAAACCCCA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453574.1
CDS	90..416
Translation	MTVQRIFRLSSVLRSAVSVHLRRNIGVTAVAFNKELDPVQKLFLDKIREYKAKRLASGGPVDTGPEYQQEVDRELFKLKQMYGKGEMDKFPTFNFEDPKFEVLDKPQS

Target ORF information:

RefSeq Version	XM_017598085.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (Atp5j), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598085.1

ORF Insert Sequence:

ATGACTGTTC AGAGGATCTT CAGGCTCTCC TCTGTCCTTC GGTCAGCAGT CTCTGTGCAT 
TTGAGGAGGA ACATTGGTGT TACAGCTGTG GCGTTTAATA AGGAACTTGA TCCTGTACAG 
AAACTCTTCT TGGACAAGAT AAGAGAGTAC AAAGCAAAGC GACTGGCGTC TGGAGGACCT 
GTTGATACTG GCCCAGAATA TCAGCAAGAG GTGGACAGAG AGCTTTTTAA GCTTAAACAA 
ATGTATGGTA AAGGAGAGAT GGATAAGTTT CCTACCTTCA ATTTTGAGGA TCCCAAATTT 
GAAGTCCTCG ACAAACCCCA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Atp5j ( XM_008768603.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008766825.1 Rattus norvegicus ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (Atp5j), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa12264
Clone ID Related Accession (Same CDS sequence)	XM_017598086.1 , XM_008768602.2 , XM_017598085.1 , NM_053602.2 , XM_008768603.1
Accession Version	XM_008768603.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ATP synthase-coupling factor 6, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005110.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGTTC AGAGGATCTT CAGGCTCTCC TCTGTCCTTC GGTCAGCAGT CTCTGTGCAT 
TTGAGGAGGA ACATTGGTGT TACAGCTGTG GCGTTTAATA AGGAACTTGA TCCTGTACAG 
AAACTCTTCT TGGACAAGAT AAGAGAGTAC AAAGCAAAGC GACTGGCGTC TGGAGGACCT 
GTTGATACTG GCCCAGAATA TCAGCAAGAG GTGGACAGAG AGCTTTTTAA GCTTAAACAA 
ATGTATGGTA AAGGAGAGAT GGATAAGTTT CCTACCTTCA ATTTTGAGGA TCCCAAATTT 
GAAGTCCTCG ACAAACCCCA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008766825.1
CDS	136..462
Translation	MTVQRIFRLSSVLRSAVSVHLRRNIGVTAVAFNKELDPVQKLFLDKIREYKAKRLASGGPVDTGPEYQQEVDRELFKLKQMYGKGEMDKFPTFNFEDPKFEVLDKPQS

Target ORF information:

RefSeq Version	XM_008768603.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6 (Atp5j), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008768603.1

ORF Insert Sequence:

ATGACTGTTC AGAGGATCTT CAGGCTCTCC TCTGTCCTTC GGTCAGCAGT CTCTGTGCAT 
TTGAGGAGGA ACATTGGTGT TACAGCTGTG GCGTTTAATA AGGAACTTGA TCCTGTACAG 
AAACTCTTCT TGGACAAGAT AAGAGAGTAC AAAGCAAAGC GACTGGCGTC TGGAGGACCT 
GTTGATACTG GCCCAGAATA TCAGCAAGAG GTGGACAGAG AGCTTTTTAA GCTTAAACAA 
ATGTATGGTA AAGGAGAGAT GGATAAGTTT CCTACCTTCA ATTTTGAGGA TCCCAAATTT 
GAAGTCCTCG ACAAACCCCA GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Marine collagen peptides reduce endothelial cell injury in diabetic rats by inhibiting apoptosis and the expression of coupling factor 6 and microparticles.
Molecular medicine reports16(4)3947-3957(2017 Oct)
Zhu C,Zhang W,Liu J,Mu B,Zhang F,Lai N,Zhou J,Xu A,Li Y

Coupling Factor 6 Is Upregulated in Monocrotaline-induced Pulmonary Arterial Hypertension in Rats.
The American journal of the medical sciences352(6)631-636(2016 Dec)
Li N,Yin J,Cai W,Liu J,Zhang N,Yan S,Song L,Li X

Lung-specific RNA interference of coupling factor 6, a novel peptide, attenuates pulmonary arterial hypertension in rats.
Respiratory research17(1)99(2016 Aug)
Yin J,You S,Li N,Jiao S,Hu H,Xue M,Wang Y,Cheng W,Liu J,Xu M,Yan S,Li X

Mitochondrial coupling factor 6 upregulation in hypertension-induced cardiac hypertrophy.
Herz40(5)783-7(2015 Aug)
He T,Guan A,Shi Y,Ge Z,Dai H

Application of a discovery to targeted LC-MS proteomics approach to identify deregulated proteins associated with idiosyncratic liver toxicity in a rat model of LPS/diclofenac co-administration.
Toxicology331100-11(2015 May)
Ramm S,Morissey B,Hernandez B,Rooney C,Pennington SR,Mally A