Col5a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col5a1
Entrez Gene ID	85490
Full Name	collagen type V alpha 1 chain
General protein information	Preferred Names collagen type V alpha 1 chain Names collagen alpha-1(V) chain collagen alpha 1 (V) collagen, type V, alpha 1 procollagen, type V, alpha 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p12
Summary	alpha chain of type V collagen; binds heparin with high affinity [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006233873.3	XP_006233935.1	collagen alpha-1(V) chain isoform X1
NM_134452.1	NP_604447.1	collagen alpha-1(V) chain precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col5a1	NP_604447.1
Homo sapiens (human)	COL5A1	NP_001265003.1
Mus musculus (house mouse)	Col5a1	NP_056549.2
Gallus gallus (chicken)	COL5A1	NP_990121.1
Xenopus tropicalis (tropical clawed frog)	col5a1	XP_004916702.1
Danio rerio (zebrafish)	col5a1	XP_002665924.2

Gene Ontology
Bibliography

Related articles in PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Barallobre-Barreiro J, Gupta SK, Zoccarato A, Kitazume-Taneike R, Fava M, Yin X, Werner T, Hirt MN, Zampetaki A, Viviano A, Chong M, Bern M, Kourliouros A, Domenech N, Willeit P, Shah AM, Jahangiri M, Schaefer L, Fischer JW, Iozzo RV, Viner R, Thum T, Heineke J, Kichler A, Otsu K, Mayr M
Circulation134(11)817-32(2016 Sep)

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.
Soteriou D, Iskender B, Byron A, Humphries JD, Borg-Bartolo S, Haddock MC, Baxter MA, Knight D, Humphries MJ, Kimber SJ
The Journal of biological chemistry288(26)18716-31(2013 Jun)

Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
Brafman DA, Phung C, Kumar N, Willert K
Cell death and differentiation20(3)369-81(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results describe the effect of collagen Va1/2 on the fibrillogenesis of Sprague-Dawley rat tenocytes, as well as the efficacy of Col V siRNA engineered tenocytes for tendon tissue engineering.
Title: Col V siRNA engineered tenocytes for tendon tissue engineering.

The following Col5a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col5a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01404	NM_134452.1 Latest version!	Rattus norvegicus collagen type V alpha 1 chain (Col5a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa25678	XM_006233873.3 Latest version!	Rattus norvegicus collagen, type V, alpha 1 (Col5a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col5a1 ( NM_134452.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_604447.1 Rattus norvegicus collagen type V alpha 1 chain (Col5a1), mRNA.

Price & Availability↑

CloneID	ORa01404
Clone ID Related Accession (Same CDS sequence)	NM_134452.1
Accession Version	NM_134452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(V) chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF272662.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF272662.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMEA2689601 [ECO:0000348] ##Evidence-Data-END##

ATGGACGTAC ACACCCGCTG GAAAGCTCCG CGCCCGGGCG CCCCGCTGCT GTCCTCGCCG 
CTGCTCCTGC TCCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAACA 
GATCTTCTGG AGATGCTAGA TTTTCACAAT TTGCCCTCAG GCGTAACGAA AACCACAGGT 
TTCTGTGCCA CTCGACGATC TTCCAAAGAG CCGGATGTTG CCTACCGAGT CTCTAAAGAT 
GCACAGCTCA GCATGCCCAC CAAGCAGCTG TACCCTGAGT CTGACTTTCC TGAGGACTTC 
TCCATCCTGA CAACCGTGAA AGCCAAGAAA GGCAGTCAGG CCTTCCTGGT CTCCGTTTAC 
AATGAGCAGG GCATCCAGCA GTTGGGACTG GAGCTGGGCC GCTCCCCTGT CTTCCTCTAT 
GAGGACCACA CAGGGAAGCC CGGGCCTGAA GAGTATCCAC TCTTCCCTGG CATCAACCTG 
TCCGATGGCA AGTGGCACCG AATCGCTATC AGTGTATACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAT CACGAAGTTC CTCAACCGCG GCGACCACCC CATAATAGAT 
GTCAACGGTA TCATCATGTT TGGCTCCCGG ATTCTGGATG ATGAAATATT TGAGGGTGAC 
ATCCAACAGT TGCTCTTCGT CTCCGACCAC CGAGCTGCCT ATGATTACTG TGAGCACTAC 
AGCCCCGACT GTGACACAGC GGTCCCTGAC ACACCTCAGT CACAGGACCC TAACCCAGAT 
GAATATTACC CAGAAGGAGA GGGTGAGACC TATTACTATG AGTACCCATA TTATGAAGAC 
CCTGAAGACC CGGGAAAGGA GCCCGCCCCC TCTCAGAAGC CAGTGGAAGC CGCCAGAGAA 
ACCACGGAGG TTCCTGAGGA GCAGACCCAG CCCCCATCCG AAGCCCCTAC AGTGCCTGAG 
ACCAGTGACA CGGCTGGCAA GGAGGACAAT CCAGGGATCG GGGACTACGA CTATGTGCCC 
ACTGACGACT ACTACACTAC ATCCCCGTAT GAAGACTTCG GCTATGGCGA GGGCGTGGAG 
AACCCCGACC AGCCCACCAA CCCTGACTCA GGGGCCGAGA TCCCCACCAG CACCAGTGTT 
ACCTCCAATT CCTCCAATCC GGCTCCATCT CCAGAGGAAG GGAAGGATGA CCTGGGAGGT 
GAGTTCACCG AGGAGACCAT CAAGAATCTA GATGAAAACT ACTACGACCC GTACTTTGAC 
CCGGACTCCG ACTCCAATGT CTCTCCATCG GAGATAGGGC CAGGCATGCC TGCTAACCAG 
GACACCATCT ATGAAGGGAT TGGAGGACCC CGAGGTGAGA AAGGGCAAAA GGGAGAACCA 
GCCATCATTG AGCCGGGAAT GCTGATAGAG GGGCCCCCTG GCCCTGAAGG CCCTGCTGGT 
CTTCCAGGAC CTCCAGGAAC TACGGGTCCT ACTGGCCAAA TGGGTGACCC TGGAGAAAGG 
GGTCCCCCTG GGCGCCCAGG TCTTCCTGGG GCTGATGGCT TGCCAGGTCC CCCCGGGACC 
ATGCTCATGC TGCCGTTCCG TTTTGGAGGT GGTGGCGACG CCGGTTCTAA GGGCCCCATG 
GTCTCTGCCC AGGAATCCCA GGCACAGGCT ATCCTTCAGC AAGCCAGGTT GGCACTAAGG 
GGACCAGCTG GTCCAATGGG TCTCACCGGG AGACCTGGCC CCATGGGTCC TCCTGGGAGT 
GGAGGTTTGA AGGGTGAGCC AGGAGACATG GGACCTCAGG GTCCTCGAGG TGTGCAAGGT 
CCACCCGGCC CAACAGGGAA GCCTGGAAGA CGGGGCCGTG CTGGGAGTGA TGGTGCCAGA 
GGCATGCCTG GACAAACAGG CCCCAAGGGT GACCGTGGCT TTGATGGTCT GGCTGGGTTG 
CCGGGAGAGA AAGGCCATAG AGGTGACCCT GGTCCTTCTG GCCCGCCGGG ACTCCCAGGA 
GATGATGGAG AAAGGGGTGA CGATGGAGAA GTTGGGCCCA GGGGGCTGCC TGGGGAGCCT 
GGACCACGTG GTCTGCTTGG GCCAAAAGGC CCCCCTGGCC CACCTGGACC TCCTGGTGTG 
ACGGGTATGG ATGGCCAGCC TGGCCCCAAA GGAAATGTGG GTCCCCAGGG AGAGCCTGGG 
CCCCCAGGAC AGCAGGGTAA TCCTGGTGCC CAGGGTCTTC CCGGTCCCCA GGGTGCCATT 
GGTCCTCCAG GAGAAAAGGG TCCTTTGGGG AAACCAGGTC TCCCAGGAAT GCCCGGAGCT 
GATGGACCCC CGGGGCACCC TGGCAAAGAA GGTCCTCCAG GAGAGAAAGG AGGCCAGGGT 
CCTCCCGGCC CCCAGGGTCC CATTGGCTAC CCAGGTCCAC GAGGAGTCAA GGGGGCAGAT 
GGCATCCGAG GGCTGAAGGG CACCAAGGGA GAGAAGGGTG AAGACGGCTT CCCTGGGTTT 
AAAGGCGACA TGGGAATAAA GGGTGACCGG GGGGAGATCG GCCCACCCGG CCCCCGAGGA 
GAAGATGGTC CTGAAGGCCC AAAAGGTCGA GGTGGTCCCA ATGGTGATCC TGGTCCCCTG 
GGGCCCACCG GGGAGAAGGG GAAGCTCGGA GTGCCAGGAT TACCAGGGTA CCCAGGAAGA 
CAAGGGCCAA AGGGTTCTAT TGGATTCCCT GGCTTCCCGG GCGCCAACGG AGAGAAAGGT 
GGCAGGGGGA CACCTGGAAA GCCAGGACCA CGGGGACAGC GAGGCCCAAC GGGCCCGAGG 
GGTGAACGAG GCCCACGGGG CATCACTGGG AAGCCTGGCC CTAAGGGCAA CTCAGGAGGT 
GATGGCCCAG CCGGCCCTCC TGGTGAGCGG GGACCCAATG GACCCCAAGG CCCCACCGGC 
TTCCCTGGAC CTAAGGGTCC CCCGGGTCCA CCAGGCAAGG ACGGGCTCCC CGGACATCCT 
GGGCAGAGAG GGGAGACTGG TTTCCAAGGC AAGACTGGCC CTCCAGGCCC TCCAGGAGTG 
GTTGGCCCTC AGGGTCCCAC AGGCGAGACG GGTCCCATGG GGGAGCGTGG CCACCCTGGT 
CCTCCAGGCC CCCCTGGTGA ACAGGGCCTC CCAGGTGCTG CTGGGAAAGA AGGGACGAAG 
GGTGACCCAG GTCCTGCTGG CCTCCCTGGG AAGGATGGAC CTCCAGGATT GCGTGGATTC 
CCTGGGGACC GAGGGCTCCC CGGCCCCGTG GGAGCACTCG GACTGAAAGG CAGCGAAGGC 
CCCCCTGGCC CCCCAGGTCC TGCGGGTTCT CCAGGGGAGA GAGGACCAGC TGGTGCTGCT 
GGACCCATTG GAATTCCAGG GAGACCTGGG CCTCAGGGAC CTCCGGGGCC TGCTGGAGAG 
AAAGGGGTTC CTGGAGAGGA AGGTCCGCAA GGCCCGGCTG GCCGAGATGG CCTGCAAGGT 
CCCGTGGGGC TCCCTGGACC AGCCGGCCCC GTGGGTCCTC CTGGAGAAGA TGGAGATAAG 
GGAGAGATCG GAGAGCCCGG GCAGAAAGGG AGCAAGGGCG ACAAAGGCGA GCAGGGTCCT 
CCTGGGCCTA CCGGTCCTCA AGGCCCCACT GGGCAGCCAG GCCCTTCGGG AGCAGATGGT 
GAACCTGGCC CTCGTGGACA GCAGGGCCTG TTTGGGCAGA AAGGAGATGA AGGTTCAAGA 
GGTTTCCCAG GACCGCCAGG GCCAGTGGGC TTGCAGGGTT TACCAGGACC TCCAGGAGAA 
AAGGGTGAGA CAGGAGACGT GGGCCAGATG GGCCCTCCTG GTCCACCAGG CCCCCGAGGA 
CCCTCTGGAG CTCCAGGAGC TGATGGGCCA CAGGGTCCTC CAGGAGGGAT TGGCAACCCT 
GGTGCAGTCG GAGAAAAGGG AGAGCCTGGC GAAGCTGGAG AGCCTGGCCT TCCAGGAGAA 
GGAGGTCCCC TGGGACCTAA AGGAGAAAGA GGGGAGAAGG GAGAGGCTGG CCCCTCTGGT 
GCTGCTGGAC CCCCTGGACC CAAAGGCCCA CCTGGAGATG ATGGCCCCAA AGGCAGCCCC 
GGCCCCGTGG GCTTTCCTGG AGACCCTGGG CCCCCTGGAG AGCCAGGCCC AGCAGGTCAA 
GATGGCCCAC CTGGTGACAA AGGGGATGAT GGAGAACCTG GGCAGACGGG ATCCCCAGGC 
CCTACTGGTG AACCTGGTCC ATCTGGGCCT CCAGGAAAGA GGGGTCCACC GGGCCCTGCA 
GGCCCTGAAG GCAGGCAGGG GGAGAAAGGA GCCAAGGGAG AAGCTGGCTT AGAAGGCCCT 
CCTGGGAAGA CTGGCCCCAT TGGTCCCCAA GGGGCCCCTG GGAAGCCTGG CCCTGATGGT 
CTTCGTGGGA TCCCTGGTCC TGTGGGTGAG CAAGGCCTCC CAGGATCCCC AGGCCCTGAT 
GGTCCACCCG GCCCCATGGG TCCTCCAGGA CTCCCTGGCC TCAAAGGAGA CTCCGGTCCC 
AAAGGTGAAA AGGGCCATCC GGGCCTGATT GGACTCATCG GACCTCCGGG CGAACAAGGT 
GAAAAAGGTG ACCGAGGACT CCCAGGCCCC CAGGGCTCAT CTGGTCCTAA AGGAGAACAG 
GGCATTACAG GTCCTTCTGG CCCGCTTGGG CCTCCTGGTC CCCCAGGCTT GCCGGGCCCT 
CCAGGTCCCA AAGGTGCTAA AGGTTCATCG GGTCCCACTG GCCCGAAGGG CGAGGCAGGC 
CATCCAGGAC TCCCTGGTCC ACCCGGGCCT CCAGGCGAGG TCATCCAGCC CCTGCCAATC 
CAGGCGTCCA GGACTCGGCG GAACATTGAT GCCAGTCAGC TCCTGGATGA TGGGGCTGGG 
GAGAGCTACG TGGATTATGC AGATGGCATG GAAGAGATCT TTGGTTCCCT CAACTCCCTG 
AAGCTGGAGA TTGAGCAGAT GAAGCGGCCA CTGGGCACAC AGCAGAACCC AGCCCGTACC 
TGCAAGGACC TTCAACTCTG CCATCCTGAT TTCCCAGACG GCGAATACTG GGTCGATCCC 
AACCAAGGGT GCTCCAGGGA CTCCTTCAAA GTCTACTGCA ATTTCACAGC TGGAGGGTCC 
ACGTGCGTCT TCCCTGACAA GAAGTCTGAG GGAGCCAGAA TCACTTCTTG GCCCAAAGAA 
AACCCAGGTT CCTGGTTCAG TGAATTCAAG CGTGGGAAAC TGCTCTCCTA TGTGGATGCT 
GAAGGCAACC CTGTGGGCGT GGTACAAATG ACCTTCCTGC GGCTGCTGAG TGCCTCTGCC 
CAGCAGAACA TCACCTACAA CTGCTACCAG TCCGTGGCCT GGCAGGATGC CGCCACAGGC 
AGCTACGATA AGGCTATCCG CTTCTTGGGC TCCAACGATG AGGAAATGTC TTATGATAAC 
AACCCCTACA TCCGTGCCCT GGTGGATGGC TGTGCTACCA AGAAAGGCTA CCAGAAGACG 
GTGCTGGAGA TTGACACGCC CAAAGTTGAG CAAGTCCCCA TTGTGGACAT CATGTTCACC 
GACTTTGGTG AAGCCTCACA GAAATTTGGA TTTGAAGTAG GGCCAGCTTG CTTCCTAGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_604447.1
CDS	6..5528
Translation	MDVHTRWKAPRPGAPLLSSPLLLLLLLLWAPPPSRAAQPTDLLEMLDFHNLPSGVTKTTGFCATRRSSKEPDVAYRVSKDAQLSMPTKQLYPESDFPEDFSILTTVKAKKGSQAFLVSVYNEQGIQQLGLELGRSPVFLYEDHTGKPGPEEYPLFPGINLSDGKWHRIAISVYKKNVTLILDCKKKITKFLNRGDHPIIDVNGIIMFGSRILDDEIFEGDIQQLLFVSDHRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYPEGEGETYYYEYPYYEDPEDPGKEPAPSQKPVEAARETTEVPEEQTQPPSEAPTVPETSDTAGKEDNPGIGDYDYVPTDDYYTTSPYEDFGYGEGVENPDQPTNPDSGAEIPTSTSVTSNSSNPAPSPEEGKDDLGGEFTEETIKNLDENYYDPYFDPDSDSNVSPSEIGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPPGTTGPTGQMGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPMGPPGSGGLKGEPGDMGPQGPRGVQGPPGPTGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGLPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPTGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGAAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGSEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGVPGEEGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPTGQPGPSGADGEPGPRGQQGLFGQKGDEGSRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPLGPKGERGEKGEAGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGEQGITGPSGPLGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHPGLPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGAGESYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGARITSWPKENPGSWFSEFKRGKLLSYVDAEGNPVGVVQMTFLRLLSASAQQNITYNCYQSVAWQDAATGSYDKAIRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFTDFGEASQKFGFEVGPACFLG

Target ORF information:

RefSeq Version	NM_134452.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type V alpha 1 chain (Col5a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134452.1

ORF Insert Sequence:

ATGGACGTAC ACACCCGCTG GAAAGCTCCG CGCCCGGGCG CCCCGCTGCT GTCCTCGCCG 
CTGCTCCTGC TCCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAACA 
GATCTTCTGG AGATGCTAGA TTTTCACAAT TTGCCCTCAG GCGTAACGAA AACCACAGGT 
TTCTGTGCCA CTCGACGATC TTCCAAAGAG CCGGATGTTG CCTACCGAGT CTCTAAAGAT 
GCACAGCTCA GCATGCCCAC CAAGCAGCTG TACCCTGAGT CTGACTTTCC TGAGGACTTC 
TCCATCCTGA CAACCGTGAA AGCCAAGAAA GGCAGTCAGG CCTTCCTGGT CTCCGTTTAC 
AATGAGCAGG GCATCCAGCA GTTGGGACTG GAGCTGGGCC GCTCCCCTGT CTTCCTCTAT 
GAGGACCACA CAGGGAAGCC CGGGCCTGAA GAGTATCCAC TCTTCCCTGG CATCAACCTG 
TCCGATGGCA AGTGGCACCG AATCGCTATC AGTGTATACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAT CACGAAGTTC CTCAACCGCG GCGACCACCC CATAATAGAT 
GTCAACGGTA TCATCATGTT TGGCTCCCGG ATTCTGGATG ATGAAATATT TGAGGGTGAC 
ATCCAACAGT TGCTCTTCGT CTCCGACCAC CGAGCTGCCT ATGATTACTG TGAGCACTAC 
AGCCCCGACT GTGACACAGC GGTCCCTGAC ACACCTCAGT CACAGGACCC TAACCCAGAT 
GAATATTACC CAGAAGGAGA GGGTGAGACC TATTACTATG AGTACCCATA TTATGAAGAC 
CCTGAAGACC CGGGAAAGGA GCCCGCCCCC TCTCAGAAGC CAGTGGAAGC CGCCAGAGAA 
ACCACGGAGG TTCCTGAGGA GCAGACCCAG CCCCCATCCG AAGCCCCTAC AGTGCCTGAG 
ACCAGTGACA CGGCTGGCAA GGAGGACAAT CCAGGGATCG GGGACTACGA CTATGTGCCC 
ACTGACGACT ACTACACTAC ATCCCCGTAT GAAGACTTCG GCTATGGCGA GGGCGTGGAG 
AACCCCGACC AGCCCACCAA CCCTGACTCA GGGGCCGAGA TCCCCACCAG CACCAGTGTT 
ACCTCCAATT CCTCCAATCC GGCTCCATCT CCAGAGGAAG GGAAGGATGA CCTGGGAGGT 
GAGTTCACCG AGGAGACCAT CAAGAATCTA GATGAAAACT ACTACGACCC GTACTTTGAC 
CCGGACTCCG ACTCCAATGT CTCTCCATCG GAGATAGGGC CAGGCATGCC TGCTAACCAG 
GACACCATCT ATGAAGGGAT TGGAGGACCC CGAGGTGAGA AAGGGCAAAA GGGAGAACCA 
GCCATCATTG AGCCGGGAAT GCTGATAGAG GGGCCCCCTG GCCCTGAAGG CCCTGCTGGT 
CTTCCAGGAC CTCCAGGAAC TACGGGTCCT ACTGGCCAAA TGGGTGACCC TGGAGAAAGG 
GGTCCCCCTG GGCGCCCAGG TCTTCCTGGG GCTGATGGCT TGCCAGGTCC CCCCGGGACC 
ATGCTCATGC TGCCGTTCCG TTTTGGAGGT GGTGGCGACG CCGGTTCTAA GGGCCCCATG 
GTCTCTGCCC AGGAATCCCA GGCACAGGCT ATCCTTCAGC AAGCCAGGTT GGCACTAAGG 
GGACCAGCTG GTCCAATGGG TCTCACCGGG AGACCTGGCC CCATGGGTCC TCCTGGGAGT 
GGAGGTTTGA AGGGTGAGCC AGGAGACATG GGACCTCAGG GTCCTCGAGG TGTGCAAGGT 
CCACCCGGCC CAACAGGGAA GCCTGGAAGA CGGGGCCGTG CTGGGAGTGA TGGTGCCAGA 
GGCATGCCTG GACAAACAGG CCCCAAGGGT GACCGTGGCT TTGATGGTCT GGCTGGGTTG 
CCGGGAGAGA AAGGCCATAG AGGTGACCCT GGTCCTTCTG GCCCGCCGGG ACTCCCAGGA 
GATGATGGAG AAAGGGGTGA CGATGGAGAA GTTGGGCCCA GGGGGCTGCC TGGGGAGCCT 
GGACCACGTG GTCTGCTTGG GCCAAAAGGC CCCCCTGGCC CACCTGGACC TCCTGGTGTG 
ACGGGTATGG ATGGCCAGCC TGGCCCCAAA GGAAATGTGG GTCCCCAGGG AGAGCCTGGG 
CCCCCAGGAC AGCAGGGTAA TCCTGGTGCC CAGGGTCTTC CCGGTCCCCA GGGTGCCATT 
GGTCCTCCAG GAGAAAAGGG TCCTTTGGGG AAACCAGGTC TCCCAGGAAT GCCCGGAGCT 
GATGGACCCC CGGGGCACCC TGGCAAAGAA GGTCCTCCAG GAGAGAAAGG AGGCCAGGGT 
CCTCCCGGCC CCCAGGGTCC CATTGGCTAC CCAGGTCCAC GAGGAGTCAA GGGGGCAGAT 
GGCATCCGAG GGCTGAAGGG CACCAAGGGA GAGAAGGGTG AAGACGGCTT CCCTGGGTTT 
AAAGGCGACA TGGGAATAAA GGGTGACCGG GGGGAGATCG GCCCACCCGG CCCCCGAGGA 
GAAGATGGTC CTGAAGGCCC AAAAGGTCGA GGTGGTCCCA ATGGTGATCC TGGTCCCCTG 
GGGCCCACCG GGGAGAAGGG GAAGCTCGGA GTGCCAGGAT TACCAGGGTA CCCAGGAAGA 
CAAGGGCCAA AGGGTTCTAT TGGATTCCCT GGCTTCCCGG GCGCCAACGG AGAGAAAGGT 
GGCAGGGGGA CACCTGGAAA GCCAGGACCA CGGGGACAGC GAGGCCCAAC GGGCCCGAGG 
GGTGAACGAG GCCCACGGGG CATCACTGGG AAGCCTGGCC CTAAGGGCAA CTCAGGAGGT 
GATGGCCCAG CCGGCCCTCC TGGTGAGCGG GGACCCAATG GACCCCAAGG CCCCACCGGC 
TTCCCTGGAC CTAAGGGTCC CCCGGGTCCA CCAGGCAAGG ACGGGCTCCC CGGACATCCT 
GGGCAGAGAG GGGAGACTGG TTTCCAAGGC AAGACTGGCC CTCCAGGCCC TCCAGGAGTG 
GTTGGCCCTC AGGGTCCCAC AGGCGAGACG GGTCCCATGG GGGAGCGTGG CCACCCTGGT 
CCTCCAGGCC CCCCTGGTGA ACAGGGCCTC CCAGGTGCTG CTGGGAAAGA AGGGACGAAG 
GGTGACCCAG GTCCTGCTGG CCTCCCTGGG AAGGATGGAC CTCCAGGATT GCGTGGATTC 
CCTGGGGACC GAGGGCTCCC CGGCCCCGTG GGAGCACTCG GACTGAAAGG CAGCGAAGGC 
CCCCCTGGCC CCCCAGGTCC TGCGGGTTCT CCAGGGGAGA GAGGACCAGC TGGTGCTGCT 
GGACCCATTG GAATTCCAGG GAGACCTGGG CCTCAGGGAC CTCCGGGGCC TGCTGGAGAG 
AAAGGGGTTC CTGGAGAGGA AGGTCCGCAA GGCCCGGCTG GCCGAGATGG CCTGCAAGGT 
CCCGTGGGGC TCCCTGGACC AGCCGGCCCC GTGGGTCCTC CTGGAGAAGA TGGAGATAAG 
GGAGAGATCG GAGAGCCCGG GCAGAAAGGG AGCAAGGGCG ACAAAGGCGA GCAGGGTCCT 
CCTGGGCCTA CCGGTCCTCA AGGCCCCACT GGGCAGCCAG GCCCTTCGGG AGCAGATGGT 
GAACCTGGCC CTCGTGGACA GCAGGGCCTG TTTGGGCAGA AAGGAGATGA AGGTTCAAGA 
GGTTTCCCAG GACCGCCAGG GCCAGTGGGC TTGCAGGGTT TACCAGGACC TCCAGGAGAA 
AAGGGTGAGA CAGGAGACGT GGGCCAGATG GGCCCTCCTG GTCCACCAGG CCCCCGAGGA 
CCCTCTGGAG CTCCAGGAGC TGATGGGCCA CAGGGTCCTC CAGGAGGGAT TGGCAACCCT 
GGTGCAGTCG GAGAAAAGGG AGAGCCTGGC GAAGCTGGAG AGCCTGGCCT TCCAGGAGAA 
GGAGGTCCCC TGGGACCTAA AGGAGAAAGA GGGGAGAAGG GAGAGGCTGG CCCCTCTGGT 
GCTGCTGGAC CCCCTGGACC CAAAGGCCCA CCTGGAGATG ATGGCCCCAA AGGCAGCCCC 
GGCCCCGTGG GCTTTCCTGG AGACCCTGGG CCCCCTGGAG AGCCAGGCCC AGCAGGTCAA 
GATGGCCCAC CTGGTGACAA AGGGGATGAT GGAGAACCTG GGCAGACGGG ATCCCCAGGC 
CCTACTGGTG AACCTGGTCC ATCTGGGCCT CCAGGAAAGA GGGGTCCACC GGGCCCTGCA 
GGCCCTGAAG GCAGGCAGGG GGAGAAAGGA GCCAAGGGAG AAGCTGGCTT AGAAGGCCCT 
CCTGGGAAGA CTGGCCCCAT TGGTCCCCAA GGGGCCCCTG GGAAGCCTGG CCCTGATGGT 
CTTCGTGGGA TCCCTGGTCC TGTGGGTGAG CAAGGCCTCC CAGGATCCCC AGGCCCTGAT 
GGTCCACCCG GCCCCATGGG TCCTCCAGGA CTCCCTGGCC TCAAAGGAGA CTCCGGTCCC 
AAAGGTGAAA AGGGCCATCC GGGCCTGATT GGACTCATCG GACCTCCGGG CGAACAAGGT 
GAAAAAGGTG ACCGAGGACT CCCAGGCCCC CAGGGCTCAT CTGGTCCTAA AGGAGAACAG 
GGCATTACAG GTCCTTCTGG CCCGCTTGGG CCTCCTGGTC CCCCAGGCTT GCCGGGCCCT 
CCAGGTCCCA AAGGTGCTAA AGGTTCATCG GGTCCCACTG GCCCGAAGGG CGAGGCAGGC 
CATCCAGGAC TCCCTGGTCC ACCCGGGCCT CCAGGCGAGG TCATCCAGCC CCTGCCAATC 
CAGGCGTCCA GGACTCGGCG GAACATTGAT GCCAGTCAGC TCCTGGATGA TGGGGCTGGG 
GAGAGCTACG TGGATTATGC AGATGGCATG GAAGAGATCT TTGGTTCCCT CAACTCCCTG 
AAGCTGGAGA TTGAGCAGAT GAAGCGGCCA CTGGGCACAC AGCAGAACCC AGCCCGTACC 
TGCAAGGACC TTCAACTCTG CCATCCTGAT TTCCCAGACG GCGAATACTG GGTCGATCCC 
AACCAAGGGT GCTCCAGGGA CTCCTTCAAA GTCTACTGCA ATTTCACAGC TGGAGGGTCC 
ACGTGCGTCT TCCCTGACAA GAAGTCTGAG GGAGCCAGAA TCACTTCTTG GCCCAAAGAA 
AACCCAGGTT CCTGGTTCAG TGAATTCAAG CGTGGGAAAC TGCTCTCCTA TGTGGATGCT 
GAAGGCAACC CTGTGGGCGT GGTACAAATG ACCTTCCTGC GGCTGCTGAG TGCCTCTGCC 
CAGCAGAACA TCACCTACAA CTGCTACCAG TCCGTGGCCT GGCAGGATGC CGCCACAGGC 
AGCTACGATA AGGCTATCCG CTTCTTGGGC TCCAACGATG AGGAAATGTC TTATGATAAC 
AACCCCTACA TCCGTGCCCT GGTGGATGGC TGTGCTACCA AGAAAGGCTA CCAGAAGACG 
GTGCTGGAGA TTGACACGCC CAAAGTTGAG CAAGTCCCCA TTGTGGACAT CATGTTCACC 
GACTTTGGTG AAGCCTCACA GAAATTTGGA TTTGAAGTAG GGCCAGCTTG CTTCCTAGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col5a1 ( XM_006233873.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233935.1 Rattus norvegicus collagen, type V, alpha 1 (Col5a1), transcript variant X1, mRNA.

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CloneID	ORa25678
Clone ID Related Accession (Same CDS sequence)	XM_006233873.3
Accession Version	XM_006233873.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-1(V) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233873.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGTAC ACACCCGCTG GAAAGCTCCG CGCCCGGGCG CCCCGCTGCT GTCCTCGCCG 
CTGCTCCTGC TCCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAACA 
GATCTTCTGG AGATGCTAGA TTTTCACAAT TTGCCCTCAG GCGTAACGAA AACCACAGGT 
TTCTGTGCCA CTCGACGATC TTCCAAAGAG CCGGATGTTG CCTACCGAGT CTCTAAAGAT 
GCACAGCTCA GCATGCCCAC CAAGCAGCTG TACCCTGAGT CTGACTTTCC TGAGGACTTC 
TCCATCCTGA CAACCGTGAA AGCCAAGAAA GGCAGTCAGG CCTTCCTGGT CTCCGTTTAC 
AATGAGCAGG GCATCCAGCA GTTGGGACTG GAGCTGGGCC GCTCCCCTGT CTTCCTCTAT 
GAGGACCACA CAGGGAAGCC CGGGCCTGAA GAGTATCCAC TCTTCCCTGG CATCAACCTG 
TCCGATGGCA AGTGGCACCG AATCGCTATC AGTGTATACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAT CACGAAGTTC CTCAACCGCG GCGACCACCC CATAATAGAT 
GTCAACGGTA TCATCATGTT TGGCTCCCGG ATTCTGGATG ATGAAATATT TGAGGGTGAC 
ATCCAACAGT TGCTCTTCGT CTCCGACCAC CGAGCTGCCT ATGATTACTG TGAGCACTAC 
AGCCCCGACT GTGACACAGC GGTCCCTGAC ACACCTCAGT CACAGGACCC TAACCCAGAT 
GAATATTACC CAGAAGGAGA GGGTGAGACC TATTACTATG AGTACCCATA TTATGAAGAC 
CCTGAAGACC CGGGAAAGGA GCCCGCCCCC TCTCAGAAGC CAGTGGAAGC CGCCAGAGAA 
ACCACGGAGG TTCCTGAGGA GCAGACCCAG CCCCCATCCG AAGCCCCTAC AGTGCCTGAG 
ACCAGTGACA CGGCTGGCAA GGAGGACAAT CCAGGGATCG GGGACTACGA CTATGTGCCC 
ACTGACGACT ACTACACTAC ATCCCCGTAT GAAGACTTCG GCTATGGCGA GGGCGTGGAG 
AACCCCGACC AGCCCACCAA CCCTGACTCA GGGGCCGAGA TCCCCACCAG CACCAGTGTT 
ACCTCCAATT CCTCCAATCC GGCTCCATCT CCAGAGGAAG GGAAGGATGA CCTGGGAGGT 
GAGTTCACCG AGGAGACCAT CAAGAATCTA GATGAAAACT ACTATGACCC GTACTTTGAC 
CCGGACTCCG ACTCCAATGT CTCTCCATCG GAGATAGGGC CAGGCATGCC TGCTAACCAG 
GACACCATCT ATGAAGGGAT TGGAGGACCC CGAGGTGAGA AAGGGCAAAA GGGAGAACCA 
GCCATCATTG AGCCGGGAAT GCTGATAGAG GGGCCCCCTG GCCCTGAAGG CCCTGCTGGT 
CTTCCAGGAC CTCCAGGAAC TACGGGTCCT ACTGGCCAAA TGGGTGACCC TGGAGAAAGG 
GGTCCCCCTG GGCGCCCAGG TCTTCCTGGG GCTGATGGCT TGCCTGGTCC CCCCGGGACC 
ATGCTCATGC TGCCGTTCCG TTTTGGAGGT GGTGGCGACG CCGGTTCTAA GGGCCCCATG 
GTCTCTGCCC AGGAATCCCA GGCACAGGCT ATCCTCCAGC AAGCCAGGTT GGCACTAAGG 
GGACCAGCTG GTCCAATGGG TCTCACCGGG AGACCTGGCC CCATGGGTCC TCCTGGGAGT 
GGAGGTTTGA AGGGTGAGCC AGGAGACATG GGACCTCAGG GTCCTCGAGG TGTGCAAGGT 
CCACCCGGCC CAACAGGGAA GCCTGGAAGA CGGGGCCGTG CTGGGAGTGA TGGTGCCAGA 
GGCATGCCTG GACAAACAGG CCCCAAGGGT GACCGTGGCT TTGATGGTCT GGCTGGGTTG 
CCGGGAGAGA AAGGCCATAG AGGTGACCCT GGTCCTTCTG GCCCGCCGGG ACTCCCAGGA 
GATGATGGAG AAAGGGGTGA CGATGGAGAA GTTGGGCCCA GGGGGCTGCC TGGGGAGCCT 
GGACCACGTG GTCTGCTTGG GCCAAAAGGC CCCCCTGGCC CACCTGGACC TCCTGGTGTG 
ACGGGTATGG ATGGCCAGCC TGGCCCCAAA GGAAATGTGG GTCCCCAGGG AGAGCCTGGG 
CCCCCAGGAC AGCAGGGTAA TCCTGGTGCC CAGGGTCTTC CCGGTCCCCA GGGTGCCATT 
GGTCCTCCAG GAGAAAAGGG TCCTTTGGGG AAACCAGGTC TCCCAGGAAT GCCCGGAGCT 
GATGGACCCC CGGGGCACCC TGGCAAAGAA GGTCCTCCAG GAGAGAAAGG AGGCCAGGGT 
CCTCCCGGCC CCCAGGGTCC CATTGGCTAC CCAGGTCCAC GAGGAGTCAA GGGGGCAGAT 
GGCATCCGAG GGCTGAAGGG CACCAAGGGA GAGAAGGGTG AAGACGGCTT CCCTGGGTTT 
AAAGGCGACA TGGGAATAAA GGGTGACCGG GGGGAGATCG GCCCACCCGG CCCCCGAGGA 
GAAGATGGTC CTGAAGGCCC AAAAGGTCGA GGTGGTCCCA ATGGTGATCC TGGTCCCCTG 
GGGCCCACCG GGGAGAAGGG AAAGCTCGGA GTGCCTGGAT TACCAGGGTA CCCAGGAAGA 
CAAGGGCCAA AGGGTTCTAT TGGATTCCCT GGCTTCCCGG GCGCCAACGG AGAGAAAGGT 
GGCAGGGGGA CACCTGGAAA GCCAGGACCA CGGGGACAGC GAGGCCCAAC GGGCCCGAGG 
GGTGAACGAG GCCCACGGGG CATCACTGGG AAGCCTGGCC CTAAGGGCAA CTCAGGAGGT 
GATGGCCCAG CCGGCCCTCC TGGTGAGCGG GGACCCAATG GACCCCAAGG CCCCACCGGC 
TTCCCTGGAC CTAAGGGTCC CCCGGGTCCA CCAGGCAAGG ACGGGCTCCC CGGACATCCT 
GGGCAGAGAG GGGAGACTGG TTTCCAAGGC AAGACTGGCC CTCCAGGCCC TCCAGGAGTG 
GTTGGCCCTC AGGGTCCCAC AGGCGAGACG GGTCCCATGG GAGAGCGTGG CCACCCTGGT 
CCTCCAGGCC CCCCTGGTGA ACAGGGCCTC CCAGGTGCTG CTGGGAAAGA AGGGACGAAG 
GGTGACCCAG GTCCTGCTGG CCTCCCTGGG AAGGATGGAC CTCCAGGATT GCGTGGATTC 
CCTGGGGACC GAGGGCTCCC CGGCCCCGTG GGAGCACTCG GACTGAAAGG CAGCGAAGGC 
CCCCCTGGCC CCCCAGGTCC TGCGGGTTCT CCAGGGGAGA GAGGACCAGC TGGTGCTGCT 
GGACCCATTG GAATTCCAGG GAGACCTGGG CCTCAGGGAC CTCCGGGGCC TGCTGGAGAG 
AAAGGGGTTC CTGGAGAGAA AGGTCCGCAA GGCCCGGCTG GCCGAGATGG CCTGCAAGGT 
CCCGTGGGGC TCCCTGGACC AGCCGGCCCC GTGGGTCCTC CTGGAGAAGA TGGAGATAAG 
GGAGAGATCG GAGAGCCCGG GCAGAAAGGG AGCAAGGGCG ACAAAGGCGA GCAGGGTCCT 
CCTGGGCCTA CCGGTCCTCA AGGCCCCATT GGGCAGCCAG GCCCTTCGGG AGCAGATGGT 
GAACCTGGCC CTCGTGGACA GCAGGGCCTG TTTGGGCAGA AAGGAGATGA AGGTTCAAGA 
GGTTTCCCAG GACCGCCAGG GCCAGTGGGC TTGCAGGGTT TACCAGGACC TCCAGGAGAA 
AAGGGTGAGA CAGGAGACGT GGGCCAGATG GGCCCTCCTG GTCCACCAGG CCCCCGAGGA 
CCCTCTGGAG CTCCAGGAGC TGATGGGCCA CAGGGTCCTC CAGGAGGGAT TGGCAACCCT 
GGTGCAGTCG GAGAAAAGGG AGAGCCTGGC GAAGCTGGAG AGCCTGGCCT TCCAGGAGAA 
GGAGGTCCCC TGGGACCTAA AGGAGAAAGA GGGGAGAAGG GAGAGGCTGG CCCCTCTGGT 
GCTGCTGGAC CCCCTGGACC CAAAGGCCCA CCTGGAGATG ATGGCCCCAA AGGCAGCCCC 
GGCCCCGTGG GCTTTCCTGG AGACCCTGGG CCCCCTGGAG AGCCAGGCCC AGCAGGTCAA 
GATGGCCCAC CTGGTGACAA AGGGGATGAT GGAGAACCTG GGCAGACGGG ATCCCCAGGC 
CCTACTGGTG AACCTGGTCC ATCTGGGCCT CCAGGAAAGA GGGGTCCACC GGGCCCTGCA 
GGCCCTGAAG GCAGGCAGGG GGAGAAAGGA GCCAAGGGAG AAGCTGGCTT AGAAGGCCCT 
CCTGGGAAGA CTGGCCCCAT TGGTCCCCAA GGGGCCCCTG GGAAGCCTGG CCCTGATGGT 
CTTCGTGGGA TCCCTGGTCC TGTGGGTGAG CAAGGCCTCC CAGGATCCCC AGGCCCTGAT 
GGTCCACCCG GCCCCATGGG TCCTCCAGGA CTCCCTGGCC TCAAAGGAGA CTCCGGTCCC 
AAAGGTGAAA AGGGCCATCC GGGCCTGATT GGACTCATCG GACCTCCGGG CGAACAAGGT 
GAAAAAGGTG ACCGAGGACT CCCAGGCCCC CAGGGCTCAT CTGGTCCTAA AGGAGAACAG 
GGCATTACAG GTCCTTCTGG CCCGCTTGGG CCTCCTGGTC CCCCAGGCTT GCCGGGCCCT 
CCAGGTCCCA AAGGTGCTAA AGGTTCATCG GGTCCCACTG GCCCGAAGGG CGAGGCAGGC 
CATCCAGGAC TCCCTGGTCC ACCCGGGCCT CCAGGCGAGG TCATCCAGCC CCTGCCAATC 
CAGGCGTCCA GGACTCGGCG GAACATTGAT GCCAGTCAGC TCCTGGATGA TGGGGCTGGG 
GAGAGCTACG TGGATTATGC AGATGGCATG GAAGAGATCT TTGGTTCCCT CAACTCCCTG 
AAGCTGGAGA TTGAGCAGAT GAAGCGGCCA CTGGGCACAC AGCAGAACCC AGCCCGTACC 
TGCAAGGACC TTCAACTCTG CCATCCTGAT TTCCCAGACG GCGAATACTG GGTCGATCCC 
AACCAAGGGT GCTCCAGGGA CTCCTTCAAA GTCTACTGCA ATTTCACAGC TGGAGGGTCC 
ACGTGCGTCT TCCCTGACAA GAAGTCTGAG GGAAGTAAAA TGGCCCGCTG GCCCAAAGAG 
CAGCCTTCCA CCTGGTATAG TCAGTACAAG CGGGGTTCCC TGCTCTCCTA TGTGGATGCT 
GAAGGCAACC CTGTGGGCGT GGTACAAATG ACCTTCCTGC GGCTGCTGAG TGCCTCTGCC 
CAGCAGAACA TCACCTACAA CTGCTACCAG TCCGTGGCCT GGCAGGATGC CGCCACAGGC 
AGCTACGATA AGGCTATCCG CTTCTTGGGC TCCAACGATG AGGAAATGTC TTATGATAAC 
AACCCCTACA TCCGTGCCCT GGTGGATGGC TGTGCTACCA AGAAAGGCTA CCAGAAGACG 
GTGCTGGAGA TTGACACGCC CAAAGTTGAG CAAGTCCCCA TTGTGGACAT CATGTTCACC 
GACTTTGGTG AAGCCTCACA GAAATTTGGA TTTGAAGTAG GGCCAGCTTG CTTCCTAGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233935.1
CDS	415..5937
Translation	MDVHTRWKAPRPGAPLLSSPLLLLLLLLWAPPPSRAAQPTDLLEMLDFHNLPSGVTKTTGFCATRRSSKEPDVAYRVSKDAQLSMPTKQLYPESDFPEDFSILTTVKAKKGSQAFLVSVYNEQGIQQLGLELGRSPVFLYEDHTGKPGPEEYPLFPGINLSDGKWHRIAISVYKKNVTLILDCKKKITKFLNRGDHPIIDVNGIIMFGSRILDDEIFEGDIQQLLFVSDHRAAYDYCEHYSPDCDTAVPDTPQSQDPNPDEYYPEGEGETYYYEYPYYEDPEDPGKEPAPSQKPVEAARETTEVPEEQTQPPSEAPTVPETSDTAGKEDNPGIGDYDYVPTDDYYTTSPYEDFGYGEGVENPDQPTNPDSGAEIPTSTSVTSNSSNPAPSPEEGKDDLGGEFTEETIKNLDENYYDPYFDPDSDSNVSPSEIGPGMPANQDTIYEGIGGPRGEKGQKGEPAIIEPGMLIEGPPGPEGPAGLPGPPGTTGPTGQMGDPGERGPPGRPGLPGADGLPGPPGTMLMLPFRFGGGGDAGSKGPMVSAQESQAQAILQQARLALRGPAGPMGLTGRPGPMGPPGSGGLKGEPGDMGPQGPRGVQGPPGPTGKPGRRGRAGSDGARGMPGQTGPKGDRGFDGLAGLPGEKGHRGDPGPSGPPGLPGDDGERGDDGEVGPRGLPGEPGPRGLLGPKGPPGPPGPPGVTGMDGQPGPKGNVGPQGEPGPPGQQGNPGAQGLPGPQGAIGPPGEKGPLGKPGLPGMPGADGPPGHPGKEGPPGEKGGQGPPGPQGPIGYPGPRGVKGADGIRGLKGTKGEKGEDGFPGFKGDMGIKGDRGEIGPPGPRGEDGPEGPKGRGGPNGDPGPLGPTGEKGKLGVPGLPGYPGRQGPKGSIGFPGFPGANGEKGGRGTPGKPGPRGQRGPTGPRGERGPRGITGKPGPKGNSGGDGPAGPPGERGPNGPQGPTGFPGPKGPPGPPGKDGLPGHPGQRGETGFQGKTGPPGPPGVVGPQGPTGETGPMGERGHPGPPGPPGEQGLPGAAGKEGTKGDPGPAGLPGKDGPPGLRGFPGDRGLPGPVGALGLKGSEGPPGPPGPAGSPGERGPAGAAGPIGIPGRPGPQGPPGPAGEKGVPGEKGPQGPAGRDGLQGPVGLPGPAGPVGPPGEDGDKGEIGEPGQKGSKGDKGEQGPPGPTGPQGPIGQPGPSGADGEPGPRGQQGLFGQKGDEGSRGFPGPPGPVGLQGLPGPPGEKGETGDVGQMGPPGPPGPRGPSGAPGADGPQGPPGGIGNPGAVGEKGEPGEAGEPGLPGEGGPLGPKGERGEKGEAGPSGAAGPPGPKGPPGDDGPKGSPGPVGFPGDPGPPGEPGPAGQDGPPGDKGDDGEPGQTGSPGPTGEPGPSGPPGKRGPPGPAGPEGRQGEKGAKGEAGLEGPPGKTGPIGPQGAPGKPGPDGLRGIPGPVGEQGLPGSPGPDGPPGPMGPPGLPGLKGDSGPKGEKGHPGLIGLIGPPGEQGEKGDRGLPGPQGSSGPKGEQGITGPSGPLGPPGPPGLPGPPGPKGAKGSSGPTGPKGEAGHPGLPGPPGPPGEVIQPLPIQASRTRRNIDASQLLDDGAGESYVDYADGMEEIFGSLNSLKLEIEQMKRPLGTQQNPARTCKDLQLCHPDFPDGEYWVDPNQGCSRDSFKVYCNFTAGGSTCVFPDKKSEGSKMARWPKEQPSTWYSQYKRGSLLSYVDAEGNPVGVVQMTFLRLLSASAQQNITYNCYQSVAWQDAATGSYDKAIRFLGSNDEEMSYDNNPYIRALVDGCATKKGYQKTVLEIDTPKVEQVPIVDIMFTDFGEASQKFGFEVGPACFLG

Target ORF information:

RefSeq Version	XM_006233873.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen, type V, alpha 1 (Col5a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233873.3

ORF Insert Sequence:

ATGGACGTAC ACACCCGCTG GAAAGCTCCG CGCCCGGGCG CCCCGCTGCT GTCCTCGCCG 
CTGCTCCTGC TCCTGCTGCT GCTGTGGGCG CCGCCTCCGA GCCGCGCAGC TCAGCCAACA 
GATCTTCTGG AGATGCTAGA TTTTCACAAT TTGCCCTCAG GCGTAACGAA AACCACAGGT 
TTCTGTGCCA CTCGACGATC TTCCAAAGAG CCGGATGTTG CCTACCGAGT CTCTAAAGAT 
GCACAGCTCA GCATGCCCAC CAAGCAGCTG TACCCTGAGT CTGACTTTCC TGAGGACTTC 
TCCATCCTGA CAACCGTGAA AGCCAAGAAA GGCAGTCAGG CCTTCCTGGT CTCCGTTTAC 
AATGAGCAGG GCATCCAGCA GTTGGGACTG GAGCTGGGCC GCTCCCCTGT CTTCCTCTAT 
GAGGACCACA CAGGGAAGCC CGGGCCTGAA GAGTATCCAC TCTTCCCTGG CATCAACCTG 
TCCGATGGCA AGTGGCACCG AATCGCTATC AGTGTATACA AGAAAAATGT CACCTTGATC 
CTCGACTGTA AAAAGAAGAT CACGAAGTTC CTCAACCGCG GCGACCACCC CATAATAGAT 
GTCAACGGTA TCATCATGTT TGGCTCCCGG ATTCTGGATG ATGAAATATT TGAGGGTGAC 
ATCCAACAGT TGCTCTTCGT CTCCGACCAC CGAGCTGCCT ATGATTACTG TGAGCACTAC 
AGCCCCGACT GTGACACAGC GGTCCCTGAC ACACCTCAGT CACAGGACCC TAACCCAGAT 
GAATATTACC CAGAAGGAGA GGGTGAGACC TATTACTATG AGTACCCATA TTATGAAGAC 
CCTGAAGACC CGGGAAAGGA GCCCGCCCCC TCTCAGAAGC CAGTGGAAGC CGCCAGAGAA 
ACCACGGAGG TTCCTGAGGA GCAGACCCAG CCCCCATCCG AAGCCCCTAC AGTGCCTGAG 
ACCAGTGACA CGGCTGGCAA GGAGGACAAT CCAGGGATCG GGGACTACGA CTATGTGCCC 
ACTGACGACT ACTACACTAC ATCCCCGTAT GAAGACTTCG GCTATGGCGA GGGCGTGGAG 
AACCCCGACC AGCCCACCAA CCCTGACTCA GGGGCCGAGA TCCCCACCAG CACCAGTGTT 
ACCTCCAATT CCTCCAATCC GGCTCCATCT CCAGAGGAAG GGAAGGATGA CCTGGGAGGT 
GAGTTCACCG AGGAGACCAT CAAGAATCTA GATGAAAACT ACTATGACCC GTACTTTGAC 
CCGGACTCCG ACTCCAATGT CTCTCCATCG GAGATAGGGC CAGGCATGCC TGCTAACCAG 
GACACCATCT ATGAAGGGAT TGGAGGACCC CGAGGTGAGA AAGGGCAAAA GGGAGAACCA 
GCCATCATTG AGCCGGGAAT GCTGATAGAG GGGCCCCCTG GCCCTGAAGG CCCTGCTGGT 
CTTCCAGGAC CTCCAGGAAC TACGGGTCCT ACTGGCCAAA TGGGTGACCC TGGAGAAAGG 
GGTCCCCCTG GGCGCCCAGG TCTTCCTGGG GCTGATGGCT TGCCTGGTCC CCCCGGGACC 
ATGCTCATGC TGCCGTTCCG TTTTGGAGGT GGTGGCGACG CCGGTTCTAA GGGCCCCATG 
GTCTCTGCCC AGGAATCCCA GGCACAGGCT ATCCTCCAGC AAGCCAGGTT GGCACTAAGG 
GGACCAGCTG GTCCAATGGG TCTCACCGGG AGACCTGGCC CCATGGGTCC TCCTGGGAGT 
GGAGGTTTGA AGGGTGAGCC AGGAGACATG GGACCTCAGG GTCCTCGAGG TGTGCAAGGT 
CCACCCGGCC CAACAGGGAA GCCTGGAAGA CGGGGCCGTG CTGGGAGTGA TGGTGCCAGA 
GGCATGCCTG GACAAACAGG CCCCAAGGGT GACCGTGGCT TTGATGGTCT GGCTGGGTTG 
CCGGGAGAGA AAGGCCATAG AGGTGACCCT GGTCCTTCTG GCCCGCCGGG ACTCCCAGGA 
GATGATGGAG AAAGGGGTGA CGATGGAGAA GTTGGGCCCA GGGGGCTGCC TGGGGAGCCT 
GGACCACGTG GTCTGCTTGG GCCAAAAGGC CCCCCTGGCC CACCTGGACC TCCTGGTGTG 
ACGGGTATGG ATGGCCAGCC TGGCCCCAAA GGAAATGTGG GTCCCCAGGG AGAGCCTGGG 
CCCCCAGGAC AGCAGGGTAA TCCTGGTGCC CAGGGTCTTC CCGGTCCCCA GGGTGCCATT 
GGTCCTCCAG GAGAAAAGGG TCCTTTGGGG AAACCAGGTC TCCCAGGAAT GCCCGGAGCT 
GATGGACCCC CGGGGCACCC TGGCAAAGAA GGTCCTCCAG GAGAGAAAGG AGGCCAGGGT 
CCTCCCGGCC CCCAGGGTCC CATTGGCTAC CCAGGTCCAC GAGGAGTCAA GGGGGCAGAT 
GGCATCCGAG GGCTGAAGGG CACCAAGGGA GAGAAGGGTG AAGACGGCTT CCCTGGGTTT 
AAAGGCGACA TGGGAATAAA GGGTGACCGG GGGGAGATCG GCCCACCCGG CCCCCGAGGA 
GAAGATGGTC CTGAAGGCCC AAAAGGTCGA GGTGGTCCCA ATGGTGATCC TGGTCCCCTG 
GGGCCCACCG GGGAGAAGGG AAAGCTCGGA GTGCCTGGAT TACCAGGGTA CCCAGGAAGA 
CAAGGGCCAA AGGGTTCTAT TGGATTCCCT GGCTTCCCGG GCGCCAACGG AGAGAAAGGT 
GGCAGGGGGA CACCTGGAAA GCCAGGACCA CGGGGACAGC GAGGCCCAAC GGGCCCGAGG 
GGTGAACGAG GCCCACGGGG CATCACTGGG AAGCCTGGCC CTAAGGGCAA CTCAGGAGGT 
GATGGCCCAG CCGGCCCTCC TGGTGAGCGG GGACCCAATG GACCCCAAGG CCCCACCGGC 
TTCCCTGGAC CTAAGGGTCC CCCGGGTCCA CCAGGCAAGG ACGGGCTCCC CGGACATCCT 
GGGCAGAGAG GGGAGACTGG TTTCCAAGGC AAGACTGGCC CTCCAGGCCC TCCAGGAGTG 
GTTGGCCCTC AGGGTCCCAC AGGCGAGACG GGTCCCATGG GAGAGCGTGG CCACCCTGGT 
CCTCCAGGCC CCCCTGGTGA ACAGGGCCTC CCAGGTGCTG CTGGGAAAGA AGGGACGAAG 
GGTGACCCAG GTCCTGCTGG CCTCCCTGGG AAGGATGGAC CTCCAGGATT GCGTGGATTC 
CCTGGGGACC GAGGGCTCCC CGGCCCCGTG GGAGCACTCG GACTGAAAGG CAGCGAAGGC 
CCCCCTGGCC CCCCAGGTCC TGCGGGTTCT CCAGGGGAGA GAGGACCAGC TGGTGCTGCT 
GGACCCATTG GAATTCCAGG GAGACCTGGG CCTCAGGGAC CTCCGGGGCC TGCTGGAGAG 
AAAGGGGTTC CTGGAGAGAA AGGTCCGCAA GGCCCGGCTG GCCGAGATGG CCTGCAAGGT 
CCCGTGGGGC TCCCTGGACC AGCCGGCCCC GTGGGTCCTC CTGGAGAAGA TGGAGATAAG 
GGAGAGATCG GAGAGCCCGG GCAGAAAGGG AGCAAGGGCG ACAAAGGCGA GCAGGGTCCT 
CCTGGGCCTA CCGGTCCTCA AGGCCCCATT GGGCAGCCAG GCCCTTCGGG AGCAGATGGT 
GAACCTGGCC CTCGTGGACA GCAGGGCCTG TTTGGGCAGA AAGGAGATGA AGGTTCAAGA 
GGTTTCCCAG GACCGCCAGG GCCAGTGGGC TTGCAGGGTT TACCAGGACC TCCAGGAGAA 
AAGGGTGAGA CAGGAGACGT GGGCCAGATG GGCCCTCCTG GTCCACCAGG CCCCCGAGGA 
CCCTCTGGAG CTCCAGGAGC TGATGGGCCA CAGGGTCCTC CAGGAGGGAT TGGCAACCCT 
GGTGCAGTCG GAGAAAAGGG AGAGCCTGGC GAAGCTGGAG AGCCTGGCCT TCCAGGAGAA 
GGAGGTCCCC TGGGACCTAA AGGAGAAAGA GGGGAGAAGG GAGAGGCTGG CCCCTCTGGT 
GCTGCTGGAC CCCCTGGACC CAAAGGCCCA CCTGGAGATG ATGGCCCCAA AGGCAGCCCC 
GGCCCCGTGG GCTTTCCTGG AGACCCTGGG CCCCCTGGAG AGCCAGGCCC AGCAGGTCAA 
GATGGCCCAC CTGGTGACAA AGGGGATGAT GGAGAACCTG GGCAGACGGG ATCCCCAGGC 
CCTACTGGTG AACCTGGTCC ATCTGGGCCT CCAGGAAAGA GGGGTCCACC GGGCCCTGCA 
GGCCCTGAAG GCAGGCAGGG GGAGAAAGGA GCCAAGGGAG AAGCTGGCTT AGAAGGCCCT 
CCTGGGAAGA CTGGCCCCAT TGGTCCCCAA GGGGCCCCTG GGAAGCCTGG CCCTGATGGT 
CTTCGTGGGA TCCCTGGTCC TGTGGGTGAG CAAGGCCTCC CAGGATCCCC AGGCCCTGAT 
GGTCCACCCG GCCCCATGGG TCCTCCAGGA CTCCCTGGCC TCAAAGGAGA CTCCGGTCCC 
AAAGGTGAAA AGGGCCATCC GGGCCTGATT GGACTCATCG GACCTCCGGG CGAACAAGGT 
GAAAAAGGTG ACCGAGGACT CCCAGGCCCC CAGGGCTCAT CTGGTCCTAA AGGAGAACAG 
GGCATTACAG GTCCTTCTGG CCCGCTTGGG CCTCCTGGTC CCCCAGGCTT GCCGGGCCCT 
CCAGGTCCCA AAGGTGCTAA AGGTTCATCG GGTCCCACTG GCCCGAAGGG CGAGGCAGGC 
CATCCAGGAC TCCCTGGTCC ACCCGGGCCT CCAGGCGAGG TCATCCAGCC CCTGCCAATC 
CAGGCGTCCA GGACTCGGCG GAACATTGAT GCCAGTCAGC TCCTGGATGA TGGGGCTGGG 
GAGAGCTACG TGGATTATGC AGATGGCATG GAAGAGATCT TTGGTTCCCT CAACTCCCTG 
AAGCTGGAGA TTGAGCAGAT GAAGCGGCCA CTGGGCACAC AGCAGAACCC AGCCCGTACC 
TGCAAGGACC TTCAACTCTG CCATCCTGAT TTCCCAGACG GCGAATACTG GGTCGATCCC 
AACCAAGGGT GCTCCAGGGA CTCCTTCAAA GTCTACTGCA ATTTCACAGC TGGAGGGTCC 
ACGTGCGTCT TCCCTGACAA GAAGTCTGAG GGAAGTAAAA TGGCCCGCTG GCCCAAAGAG 
CAGCCTTCCA CCTGGTATAG TCAGTACAAG CGGGGTTCCC TGCTCTCCTA TGTGGATGCT 
GAAGGCAACC CTGTGGGCGT GGTACAAATG ACCTTCCTGC GGCTGCTGAG TGCCTCTGCC 
CAGCAGAACA TCACCTACAA CTGCTACCAG TCCGTGGCCT GGCAGGATGC CGCCACAGGC 
AGCTACGATA AGGCTATCCG CTTCTTGGGC TCCAACGATG AGGAAATGTC TTATGATAAC 
AACCCCTACA TCCGTGCCCT GGTGGATGGC TGTGCTACCA AGAAAGGCTA CCAGAAGACG 
GTGCTGGAGA TTGACACGCC CAAAGTTGAG CAAGTCCCCA TTGTGGACAT CATGTTCACC 
GACTTTGGTG AAGCCTCACA GAAATTTGGA TTTGAAGTAG GGCCAGCTTG CTTCCTAGGC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glycoproteomics Reveals Decorin Peptides With Anti-Myostatin Activity in Human Atrial Fibrillation.
Circulation134(11)817-32(2016 Sep)
Barallobre-Barreiro J,Gupta SK,Zoccarato A,Kitazume-Taneike R,Fava M,Yin X,Werner T,Hirt MN,Zampetaki A,Viviano A,Chong M,Bern M,Kourliouros A,Domenech N,Willeit P,Shah AM,Jahangiri M,Schaefer L,Fischer JW,Iozzo RV,Viner R,Thum T,Heineke J,Kichler A,Otsu K,Mayr M

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.
The Journal of biological chemistry288(26)18716-31(2013 Jun)
Soteriou D,Iskender B,Byron A,Humphries JD,Borg-Bartolo S,Haddock MC,Baxter MA,Knight D,Humphries MJ,Kimber SJ

Regulation of endodermal differentiation of human embryonic stem cells through integrin-ECM interactions.
Cell death and differentiation20(3)369-81(2013 Mar)
Brafman DA,Phung C,Kumar N,Willert K

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Col5a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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