Ajuba cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ajuba
Entrez Gene ID	85265
Full Name	ajuba LIM protein
Synonyms	Jub
General protein information	Preferred Names ajuba LIM protein Names LIM domain-containing protein ajuba Ajuba protein ajuba homolog jub, ajuba homolog protein ajuba
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p13
Summary	cytoplasmic LIM protein that binds glial glutamate transporter GLT-1 and is proposed to allow glial glutamate transporter GLT-1 to regulate intracellular signaling or interact with the cytoskeleton [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053503.1	NP_445955.1	LIM domain-containing protein ajuba

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AJUBA	NP_001179446.1
Homo sapiens (human)	AJUBA	NP_116265.1
	JUB	XP_001104222.1
Canis lupus familiaris (dog)	AJUBA	XP_005623277.1
Mus musculus (house mouse)	Ajuba	NP_034720.2
Rattus norvegicus (Norway rat)	Ajuba	NP_445955.1

Gene Ontology
Bibliography

Related articles in PubMed

The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity.
Foxler DE, Bridge KS, James V, Webb TM, Mee M, Wong SC, Feng Y, Constantin-Teodosiu D, Petursdottir TE, Bjornsson J, Ingvarsson S, Ratcliffe PJ, Longmore GD, Sharp TV
Nature cell biology14(2)201-8(2012 Jan)

LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing.
James V, Zhang Y, Foxler DE, de Moor CH, Kong YW, Webb TM, Self TJ, Feng Y, Lagos D, Chu CY, Rana TM, Morley SJ, Longmore GD, Bushell M, Sharp TV
Proceedings of the National Academy of Sciences of the United States of America107(28)12499-504(2010 Jul)

Ajuba LIM proteins are negative regulators of the Hippo signaling pathway.
Das Thakur M, Feng Y, Jagannathan R, Seppa MJ, Skeath JB, Longmore GD
Current biology : CB20(7)657-62(2010 Apr)

Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis.
Zheng P, Dean J
Proceedings of the National Academy of Sciences of the United States of America106(18)7473-8(2009 May)

Ajuba functions as a histone deacetylase-dependent co-repressor for autoregulation of the growth factor-independent-1 transcription factor.
Montoya-Durango DE, Velu CS, Kazanjian A, Rojas ME, Jay CM, Longmore GD, Grimes HL
The Journal of biological chemistry283(46)32056-65(2008 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We propose that Ajuba is a scaffolding protein allowing GLT-1 to regulate intracellular signaling or interact with the cytoskeleton.
Title: The amino terminus of the glial glutamate transporter GLT-1 interacts with the LIM protein Ajuba.

The following Ajuba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ajuba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09739	NM_053503.1 Latest version!	Rattus norvegicus ajuba LIM protein (Ajuba), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$321.30 $459.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ajuba ( NM_053503.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445955.1 Rattus norvegicus ajuba LIM protein (Ajuba), mRNA.

Price & Availability↑

CloneID	ORa09739
Clone ID Related Accession (Same CDS sequence)	NM_053503.1
Accession Version	NM_053503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	LIM domain-containing protein ajuba
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC085802.1. On Nov 22, 2004 this sequence version replaced XM_341313.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085802.1, AJ306292.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCGGT TAGGGGAGAA AGCCAGTCGT CTGCTAGAAA AGTTGAGACT TTCGGACTCC 
GGCAGCGCCA AGTTCGGCCG CAGAAAGGGC GAAGCGAGCC GGTCTGGGTC TGATGGGACC 
CCCGGAGCAG GCAAGGGACG CCTGAGTGGG CTAGGGGGAC CTAGGAAATC AGGACACCGA 
GGAGCGAATG GGGGGCCTGG GGATGAAGCT TTGGAACCGG CCAGGGAGCA AGGGCCCCTG 
GACGCCGAGC GGAACGCACG CGGCTCCTTT GAAGCGCAGC GCTTCGAAGG GTCCTTTCCC 
GGGGGGCCGC CGCCCACTCG AGCCTTGCCT CTGCCCCTGT CGTCGCCTCC TGATTTTCGG 
TTGGAGACCA CGGCTCCAGC CCTTAGCCCT CGCTCCAGCT TCGCCAGTAG CTCGGCCAGC 
GATGCGAGCA AGCCATCCAG CCCCCGGGGC AGCCTGCTGC TGGACGGAGC GGGGGCCAGC 
GGAGCCGGTG GTAGCCGCCC GTGCAGCAAT CGGACCAGCG GCATCAGCAT GGGCTACGAC 
CAGCGCCACG GGAGCCCGCT GCCTGCGGGG CCGTGCCTAT TTGGCCTCCC CCTGACCACT 
GCTCCTTCGG GCTACTCCTC CGGAGGGGTT CCATCCGCCT ATCCGGAACT CCACGCTGCC 
CTCGACCGAC TATGCGCTCA TCGGCCGGTG GGTTTCGGCT GCCAGGAGAG CCGTCACTCT 
TACCCCCCGG CCCTGGGCAG CCCCGGAGCT CTAACCGGAG CCGTGGTGGG AACAGCGGGT 
CCTTTGGAGA GACGTGGGAC GCAACCCGGA CGTCACTCGG TTACGGGCTA CGGGGACTGC 
GCCGCGGGAG CCCGTTACCA GGACGAGCTA ACAGCACTGC TGCGTTTGAC GGTGGCTACC 
GGTGGGCGAG AAGCCGGTGC CCGCGGGGAA CCCTTGGGGA TTGAGCCGTC GGGTCTGGAG 
GAGTCTCCTG GTTCCTTCGT TCCAGAGGCC TCCCGATCCC GGATACGGGA GCCAGAGGCC 
AGAGAAGATT ACTTTGGCAC CTGTATCAAG TGCAACAAAG GTATCTATGG GCAGAGCAAT 
GCCTGCCAGG CCCTGGACAG CCTCTACCAC ACCCAGTGCT TTGTCTGCTG CTCCTGTGGA 
CGAACTTTGC GGTGCAAGGC TTTCTACAGC GTCAACGGCT CTGTCTACTG TGAGGAAGAT 
TATCTGTTTT CAGGGTTTCA GGAGGCAGCT GAGAAGTGCT GTGTCTGTGG CCACTTGATT 
CTAGAGAAGA TCCTCCAGGC CATGGGGAAG TCCTATCATC CAGGCTGCTT CCGGTGCATC 
GTTTGTAACA AGTGCCTGGA CGGCGTCCCC TTCACTGTGG ACTTCTCCAA CCAGGTGTAC 
TGTGTGACTG ACTACCACAA AAATTACGCC CCGAAATGTG CAGCCTGTGG ACAGCCCATC 
CTCCCCTCAG AGGGCTGTGA GGACATTGTG AGGGTGATAT CCATGGACCG TGATTATCAC 
TTTGAGTGCT ACCACTGTGA GGACTGCCGC ATGCAGCTGA GTGACGAGGA AGGTTGCTGC 
TGTTTCCCCC TTGACGGACA TTTGCTCTGC CACGGCTGTC ACATGCAGCG CCTCAGTGCC 
CGACAGCCCC CTACCAACTA TATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445955.1
CDS	323..1969
Translation	MERLGEKASRLLEKLRLSDSGSAKFGRRKGEASRSGSDGTPGAGKGRLSGLGGPRKSGHRGANGGPGDEALEPAREQGPLDAERNARGSFEAQRFEGSFPGGPPPTRALPLPLSSPPDFRLETTAPALSPRSSFASSSASDASKPSSPRGSLLLDGAGASGAGGSRPCSNRTSGISMGYDQRHGSPLPAGPCLFGLPLTTAPSGYSSGGVPSAYPELHAALDRLCAHRPVGFGCQESRHSYPPALGSPGALTGAVVGTAGPLERRGTQPGRHSVTGYGDCAAGARYQDELTALLRLTVATGGREAGARGEPLGIEPSGLEESPGSFVPEASRSRIREPEAREDYFGTCIKCNKGIYGQSNACQALDSLYHTQCFVCCSCGRTLRCKAFYSVNGSVYCEEDYLFSGFQEAAEKCCVCGHLILEKILQAMGKSYHPGCFRCIVCNKCLDGVPFTVDFSNQVYCVTDYHKNYAPKCAACGQPILPSEGCEDIVRVISMDRDYHFECYHCEDCRMQLSDEEGCCCFPLDGHLLCHGCHMQRLSARQPPTNYI

Target ORF information:

RefSeq Version	NM_053503.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ajuba LIM protein (Ajuba), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053503.1

ORF Insert Sequence:

ATGGAGCGGT TAGGGGAGAA AGCCAGTCGT CTGCTAGAAA AGTTGAGACT TTCGGACTCC 
GGCAGCGCCA AGTTCGGCCG CAGAAAGGGC GAAGCGAGCC GGTCTGGGTC TGATGGGACC 
CCCGGAGCAG GCAAGGGACG CCTGAGTGGG CTAGGGGGAC CTAGGAAATC AGGACACCGA 
GGAGCGAATG GGGGGCCTGG GGATGAAGCT TTGGAACCGG CCAGGGAGCA AGGGCCCCTG 
GACGCCGAGC GGAACGCACG CGGCTCCTTT GAAGCGCAGC GCTTCGAAGG GTCCTTTCCC 
GGGGGGCCGC CGCCCACTCG AGCCTTGCCT CTGCCCCTGT CGTCGCCTCC TGATTTTCGG 
TTGGAGACCA CGGCTCCAGC CCTTAGCCCT CGCTCCAGCT TCGCCAGTAG CTCGGCCAGC 
GATGCGAGCA AGCCATCCAG CCCCCGGGGC AGCCTGCTGC TGGACGGAGC GGGGGCCAGC 
GGAGCCGGTG GTAGCCGCCC GTGCAGCAAT CGGACCAGCG GCATCAGCAT GGGCTACGAC 
CAGCGCCACG GGAGCCCGCT GCCTGCGGGG CCGTGCCTAT TTGGCCTCCC CCTGACCACT 
GCTCCTTCGG GCTACTCCTC CGGAGGGGTT CCATCCGCCT ATCCGGAACT CCACGCTGCC 
CTCGACCGAC TATGCGCTCA TCGGCCGGTG GGTTTCGGCT GCCAGGAGAG CCGTCACTCT 
TACCCCCCGG CCCTGGGCAG CCCCGGAGCT CTAACCGGAG CCGTGGTGGG AACAGCGGGT 
CCTTTGGAGA GACGTGGGAC GCAACCCGGA CGTCACTCGG TTACGGGCTA CGGGGACTGC 
GCCGCGGGAG CCCGTTACCA GGACGAGCTA ACAGCACTGC TGCGTTTGAC GGTGGCTACC 
GGTGGGCGAG AAGCCGGTGC CCGCGGGGAA CCCTTGGGGA TTGAGCCGTC GGGTCTGGAG 
GAGTCTCCTG GTTCCTTCGT TCCAGAGGCC TCCCGATCCC GGATACGGGA GCCAGAGGCC 
AGAGAAGATT ACTTTGGCAC CTGTATCAAG TGCAACAAAG GTATCTATGG GCAGAGCAAT 
GCCTGCCAGG CCCTGGACAG CCTCTACCAC ACCCAGTGCT TTGTCTGCTG CTCCTGTGGA 
CGAACTTTGC GGTGCAAGGC TTTCTACAGC GTCAACGGCT CTGTCTACTG TGAGGAAGAT 
TATCTGTTTT CAGGGTTTCA GGAGGCAGCT GAGAAGTGCT GTGTCTGTGG CCACTTGATT 
CTAGAGAAGA TCCTCCAGGC CATGGGGAAG TCCTATCATC CAGGCTGCTT CCGGTGCATC 
GTTTGTAACA AGTGCCTGGA CGGCGTCCCC TTCACTGTGG ACTTCTCCAA CCAGGTGTAC 
TGTGTGACTG ACTACCACAA AAATTACGCC CCGAAATGTG CAGCCTGTGG ACAGCCCATC 
CTCCCCTCAG AGGGCTGTGA GGACATTGTG AGGGTGATAT CCATGGACCG TGATTATCAC 
TTTGAGTGCT ACCACTGTGA GGACTGCCGC ATGCAGCTGA GTGACGAGGA AGGTTGCTGC 
TGTTTCCCCC TTGACGGACA TTTGCTCTGC CACGGCTGTC ACATGCAGCG CCTCAGTGCC 
CGACAGCCCC CTACCAACTA TATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity.
Nature cell biology14(2)201-8(2012 Jan)
Foxler DE,Bridge KS,James V,Webb TM,Mee M,Wong SC,Feng Y,Constantin-Teodosiu D,Petursdottir TE,Bjornsson J,Ingvarsson S,Ratcliffe PJ,Longmore GD,Sharp TV

LIM-domain proteins, LIMD1, Ajuba, and WTIP are required for microRNA-mediated gene silencing.
Proceedings of the National Academy of Sciences of the United States of America107(28)12499-504(2010 Jul)
James V,Zhang Y,Foxler DE,de Moor CH,Kong YW,Webb TM,Self TJ,Feng Y,Lagos D,Chu CY,Rana TM,Morley SJ,Longmore GD,Bushell M,Sharp TV

Ajuba LIM proteins are negative regulators of the Hippo signaling pathway.
Current biology : CB20(7)657-62(2010 Apr)
Das Thakur M,Feng Y,Jagannathan R,Seppa MJ,Skeath JB,Longmore GD

Role of Filia, a maternal effect gene, in maintaining euploidy during cleavage-stage mouse embryogenesis.
Proceedings of the National Academy of Sciences of the United States of America106(18)7473-8(2009 May)
Zheng P,Dean J

Ajuba functions as a histone deacetylase-dependent co-repressor for autoregulation of the growth factor-independent-1 transcription factor.
The Journal of biological chemistry283(46)32056-65(2008 Nov)
Montoya-Durango DE,Velu CS,Kazanjian A,Rojas ME,Jay CM,Longmore GD,Grimes HL