Soat1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Soat1
Entrez Gene ID	81782
Full Name	sterol O-acyltransferase 1
Synonyms	Acat-1
General protein information	Preferred Names sterol O-acyltransferase 1 Names sterol O-acyltransferase 1 acyl-coenzyme A:cholesterol acyltransferase 1 cholesterol acyltransferase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q22
Summary	catalyzes the conversion of free cholesterol and fatty acyl-CoA to cholesteryl esters; plays a role in cholesterol metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031118.1	NP_112380.1	sterol O-acyltransferase 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SOAT1	NP_001029378.1
Drosophila melanogaster (fruit fly)	CG8112	NP_731269.2
Caenorhabditis elegans (roundworm)	mboa-1	NP_510623.1
Rattus norvegicus (Norway rat)	Soat1	NP_112380.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012216	XP_320321.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012217	XP_320320.4
Homo sapiens (human)	SOAT1	NP_003092.4
Canis lupus familiaris (dog)	LOC490325	XP_547445.2
Mus musculus (house mouse)	Soat1	NP_033256.2
Danio rerio (zebrafish)	soat1	NP_001116744.1
Xenopus tropicalis (tropical clawed frog)	soat1	NP_001120236.1
Pan troglodytes (chimpanzee)	SOAT1	XP_514030.2
	SOAT1	XP_002802084.1
Gallus gallus (chicken)	SOAT1	XP_004943294.1

Gene Ontology
Bibliography

Related articles in PubMed

Knockdown of ACAT-1 reduces amyloidogenic processing of APP.
Huttunen HJ, Greco C, Kovacs DM
FEBS letters581(8)1688-92(2007 Apr)

A critical role for the histidine residues in the catalytic function of acyl-CoA:cholesterol acyltransferase catalysis: evidence for catalytic difference between ACAT1 and ACAT2.
An S, Cho KH, Lee WS, Lee JO, Paik YK, Jeong TS
FEBS letters580(11)2741-9(2006 May)

Altered hepatic cholesterol metabolism compensates for disruption of phosphatidylcholine transfer protein in mice.
Wu MK, Cohen DE
American journal of physiology. Gastrointestinal and liver physiology289(3)G456-61(2005 Sep)

Overexpression of human diacylglycerol acyltransferase 1, acyl-coa:cholesterol acyltransferase 1, or acyl-CoA:cholesterol acyltransferase 2 stimulates secretion of apolipoprotein B-containing lipoproteins in McA-RH7777 cells.
Liang JJ, Oelkers P, Guo C, Chu PC, Dixon JL, Ginsberg HN, Sturley SL
The Journal of biological chemistry279(43)44938-44(2004 Oct)

Enhancement of human ACAT1 gene expression to promote the macrophage-derived foam cell formation by dexamethasone.
Yang L, Yang JB, Chen J, Yu GY, Zhou P, Lei L, Wang ZZ, Cy Chang C, Yang XY, Chang TY, Li BL
Cell research14(4)315-23(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

increasing DGAT1, ACAT1, or ACAT2 expression stimulates the assembly and secretion of VLDL from liver cells
Title: Overexpression of human diacylglycerol acyltransferase 1, acyl-coa:cholesterol acyltransferase 1, or acyl-CoA:cholesterol acyltransferase 2 stimulates secretion of apolipoprotein B-containing lipoproteins in McA-RH7777 cells.

microsomal ACAT forms cholesteryl esters independent of cholesterol binding/transfer ability, modulated by ACBP
Title: ACBP and cholesterol differentially alter fatty acyl CoA utilization by microsomal ACAT.

Upregulation of acyl-CoA: cholesterol acyltransferase in chronic renal failure.
Title: Upregulation of acyl-CoA: cholesterol acyltransferase in chronic renal failure.

Up-regulation of acyl-coenzyme A:cholesterol acyltransferase (ACAT) in nephrotic syndrome.
Title: Up-regulation of acyl-coenzyme A:cholesterol acyltransferase (ACAT) in nephrotic syndrome.

The following Soat1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Soat1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13049	NM_031118.1 Latest version!	Rattus norvegicus sterol O-acyltransferase 1 (Soat1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Soat1 ( NM_031118.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112380.1 Rattus norvegicus sterol O-acyltransferase 1 (Soat1), mRNA.

Price & Availability↑

CloneID	ORa13049
Clone ID Related Accession (Same CDS sequence)	NM_031118.1
Accession Version	NM_031118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	sterol O-acyltransferase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D86373.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D86373.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906352 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGTGGGAG AAGAAACGTC TCTGAGAAAC CGGCTGTCAA GATCTGCTGA GAATCCTGAG 
CAAGATGAAG CCCAGAAAAA TCTCTTGGAC ACACACAGAA ATGGTCACAT TACCATGAAA 
CAGTTGATAG CCAAGAAGAG GCAGTTGGCG GCAGAGGCAG AGGAGCTGAA GCCACTGTTT 
CTGAAGGAAG TTGGGTGTCA CTTTGATGAC TTTGTGACCA ATCTCATTGA CAAGTCTGCA 
TCTCTGGACA ATGGCGGGTG TGCGCTCACG ACCTTCTCCA TTCTTGAAGA AATGAAAAAC 
AACCACAGAG CCAAAGATCT GAGAGCACCT CCAGAACAAG GGAAGATATT TATTTCAAGG 
CGGTCTCTTT TAGACGAGCT GTTTGAAGTG GACCACATCA GAACAATTTA CCACATGTTC 
ATTGCCCTCC TCATCATCTT CATCCTTAGC ACGCTCGTGG TGGATTACAT CGATGAAGGA 
AGGCTGGTCC TTGAGTTCAG TCTCCTGGCT TATGCTTTTG GCCAATTTCC TATTGTTATT 
TGGACATGGT GGGCCATGTT CCTGTCTACA CTGGCAATCC CCTATTTCCT GTTCCAGCGT 
TGGGCCCATG GTTACAGCAA GAGTTCCCAC CCACTCATCT ATTCCCTTAT CCATGGCGCA 
TTCTTCTTGG TCTTTCAACT TGGAATCCTC GGTTTTATAC CAACATATGT TGTCTTAGCA 
TACACGCTGC CCCCAGCCTC CCGGTTCATT CTGATACTTG AGCAGATTCG TTTGGTAATG 
AAGGCTCACT CATATGTCAG AGAGAATGTG CCACGAGTTC TAAGTGCAGC CAAGGAGAAA 
TCGAGCACGG TTCCAGTACC CACAGTCAAC CAGTACCTGT ACTTCCTGTT TGCTCCTACA 
CTTATTTACC GAGACAGCTA CCCCAGGACT CCTACTGTAA GATGGGGTTA TGTTGCTATG 
CAGTTTTTAC AGGTTTTTGG TTGCCTGTTT TATGTGTACT ACATCTTTGA GAGACTCTGT 
GCCCCTCTGT TCCGGAATAT CAAACAGGAG CCCTTTAGTG CTCGTGTCCT GGTCCTGTGT 
GTATTTAACT CCATCTTGCC AGGTGTGCTG ATGCTGTTCC TTTCGTTCTT TGCCTTTTTG 
CACTGCTGGC TCAATGCCTT TGCTGAAATG TTACGCTTTG GTGACAGGAT GTTTTATAAG 
GACTGGTGGA ACTCTACATC GTACTCCAAC TACTACAGGA CCTGGAATGT GGTGGTCCAC 
GACTGGCTCT ACTACTATGT TTACAAGGAC CTCCTCTGGT TTTTCTCGAA GAGGTTCAGG 
CCTGCCGCCA TGTTGGCCGT GTTCGCCCTG TCAGCAGTGG TGCACGAGTA CGCCCTCGCT 
GTCTGCCTGA GCTACTTCTA CCCAGTGCTC TTCGTGCTCT TCATGTTCTT TGGAATGGCT 
TTTAACTTCA TCGTTAACGA CAGTCGGAAA AGGCCAGTCT GGAACATCAT GGTTCGGGCT 
TCCCTCTTCC TCGGCCATGG AGTCATTCTG TGCTTTTACT CTCAAGAGTG GTATGCTCGA 
CAGCGCTGTC CTCTGAAGAA CCCTACATTT CTGGATTATG TCCGGCCACG GACCTGGACT 
TGTCGTTACG TGTTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112380.1
CDS	92..1729
Translation	MVGEETSLRNRLSRSAENPEQDEAQKNLLDTHRNGHITMKQLIAKKRQLAAEAEELKPLFLKEVGCHFDDFVTNLIDKSASLDNGGCALTTFSILEEMKNNHRAKDLRAPPEQGKIFISRRSLLDELFEVDHIRTIYHMFIALLIIFILSTLVVDYIDEGRLVLEFSLLAYAFGQFPIVIWTWWAMFLSTLAIPYFLFQRWAHGYSKSSHPLIYSLIHGAFFLVFQLGILGFIPTYVVLAYTLPPASRFILILEQIRLVMKAHSYVRENVPRVLSAAKEKSSTVPVPTVNQYLYFLFAPTLIYRDSYPRTPTVRWGYVAMQFLQVFGCLFYVYYIFERLCAPLFRNIKQEPFSARVLVLCVFNSILPGVLMLFLSFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYYRTWNVVVHDWLYYYVYKDLLWFFSKRFRPAAMLAVFALSAVVHEYALAVCLSYFYPVLFVLFMFFGMAFNFIVNDSRKRPVWNIMVRASLFLGHGVILCFYSQEWYARQRCPLKNPTFLDYVRPRTWTCRYVF

Target ORF information:

RefSeq Version	NM_031118.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sterol O-acyltransferase 1 (Soat1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031118.1

ORF Insert Sequence:

ATGGTGGGAG AAGAAACGTC TCTGAGAAAC CGGCTGTCAA GATCTGCTGA GAATCCTGAG 
CAAGATGAAG CCCAGAAAAA TCTCTTGGAC ACACACAGAA ATGGTCACAT TACCATGAAA 
CAGTTGATAG CCAAGAAGAG GCAGTTGGCG GCAGAGGCAG AGGAGCTGAA GCCACTGTTT 
CTGAAGGAAG TTGGGTGTCA CTTTGATGAC TTTGTGACCA ATCTCATTGA CAAGTCTGCA 
TCTCTGGACA ATGGCGGGTG TGCGCTCACG ACCTTCTCCA TTCTTGAAGA AATGAAAAAC 
AACCACAGAG CCAAAGATCT GAGAGCACCT CCAGAACAAG GGAAGATATT TATTTCAAGG 
CGGTCTCTTT TAGACGAGCT GTTTGAAGTG GACCACATCA GAACAATTTA CCACATGTTC 
ATTGCCCTCC TCATCATCTT CATCCTTAGC ACGCTCGTGG TGGATTACAT CGATGAAGGA 
AGGCTGGTCC TTGAGTTCAG TCTCCTGGCT TATGCTTTTG GCCAATTTCC TATTGTTATT 
TGGACATGGT GGGCCATGTT CCTGTCTACA CTGGCAATCC CCTATTTCCT GTTCCAGCGT 
TGGGCCCATG GTTACAGCAA GAGTTCCCAC CCACTCATCT ATTCCCTTAT CCATGGCGCA 
TTCTTCTTGG TCTTTCAACT TGGAATCCTC GGTTTTATAC CAACATATGT TGTCTTAGCA 
TACACGCTGC CCCCAGCCTC CCGGTTCATT CTGATACTTG AGCAGATTCG TTTGGTAATG 
AAGGCTCACT CATATGTCAG AGAGAATGTG CCACGAGTTC TAAGTGCAGC CAAGGAGAAA 
TCGAGCACGG TTCCAGTACC CACAGTCAAC CAGTACCTGT ACTTCCTGTT TGCTCCTACA 
CTTATTTACC GAGACAGCTA CCCCAGGACT CCTACTGTAA GATGGGGTTA TGTTGCTATG 
CAGTTTTTAC AGGTTTTTGG TTGCCTGTTT TATGTGTACT ACATCTTTGA GAGACTCTGT 
GCCCCTCTGT TCCGGAATAT CAAACAGGAG CCCTTTAGTG CTCGTGTCCT GGTCCTGTGT 
GTATTTAACT CCATCTTGCC AGGTGTGCTG ATGCTGTTCC TTTCGTTCTT TGCCTTTTTG 
CACTGCTGGC TCAATGCCTT TGCTGAAATG TTACGCTTTG GTGACAGGAT GTTTTATAAG 
GACTGGTGGA ACTCTACATC GTACTCCAAC TACTACAGGA CCTGGAATGT GGTGGTCCAC 
GACTGGCTCT ACTACTATGT TTACAAGGAC CTCCTCTGGT TTTTCTCGAA GAGGTTCAGG 
CCTGCCGCCA TGTTGGCCGT GTTCGCCCTG TCAGCAGTGG TGCACGAGTA CGCCCTCGCT 
GTCTGCCTGA GCTACTTCTA CCCAGTGCTC TTCGTGCTCT TCATGTTCTT TGGAATGGCT 
TTTAACTTCA TCGTTAACGA CAGTCGGAAA AGGCCAGTCT GGAACATCAT GGTTCGGGCT 
TCCCTCTTCC TCGGCCATGG AGTCATTCTG TGCTTTTACT CTCAAGAGTG GTATGCTCGA 
CAGCGCTGTC CTCTGAAGAA CCCTACATTT CTGGATTATG TCCGGCCACG GACCTGGACT 
TGTCGTTACG TGTTTTAG