Eppin cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Eppin
Entrez Gene ID	685161
Full Name	epididymal peptidase inhibitor
Synonyms	Spinlw1,Wfdc8
General protein information	Preferred Names epididymal peptidase inhibitor Names eppin WAP four-disulfide core domain protein 8 epididymal protease inhibitor serine peptidase inhibitor-like, with Kunitz and WAP domains 1 (eppin) serine protease inhibitor-like with Kunitz and WAP domains 1 serine protease inhibitor-like, with Kunitz and WAP domains 1 (eppin)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q42

mRNA and Protein(s)

mRNA	Protein	Name
NM_001109457.1	NP_001102927.1	eppin precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Eppin	NP_083601.1
Rattus norvegicus (Norway rat)	Eppin	NP_001102927.1
Bos taurus (cattle)	SPINLW1	NP_001070530.1
Homo sapiens (human)	EPPIN-WFDC6	NP_001185915.1
Pan troglodytes (chimpanzee)	SPINLW1	XP_514679.3

Gene Ontology
Bibliography

Related articles in PubMed

De novo assembly of the transcriptome of Acanthaster planci testes.
Stewart MJ, Stewart P, Rivera-Posada J
Molecular ecology resources15(4)953-66(2015 Jul)

Epididymal protease inhibitor (EPPIN) is differentially expressed in the male rat reproductive tract and immunolocalized in maturing spermatozoa.
Silva EJ, Patr?o MT, Tsuruta JK, O'Rand MG, Avellar MC
Molecular reproduction and development79(12)832-42(2012 Dec)

Loss of calcium in human spermatozoa via EPPIN, the semenogelin receptor.
O'Rand MG, Widgren EE
Biology of reproduction86(2)55(2012 Feb)

Identification and characterization of Wfdc gene expression in the male reproductive tract of the rat.
Rajesh A, Madhubabu G, Yenugu S
Molecular reproduction and development78(9)633-41(2011 Sep)

Functional domains of the human epididymal protease inhibitor, eppin.
McCrudden MT, Dafforn TR, Houston DF, Turkington PT, Timson DJ
The FEBS journal275(8)1742-50(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

data suggested that EPPIN expression in rats is more widespread than in humans and mice, and is androgen-dependent in the epididymis; findings provide new insights into its potential physiological roles in reproduction
Title: Epididymal protease inhibitor (EPPIN) is differentially expressed in the male rat reproductive tract and immunolocalized in maturing spermatozoa.

The following Eppin gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eppin cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09989	NM_001109457.1 Latest version!	Rattus norvegicus epididymal peptidase inhibitor (Eppin), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Eppin ( NM_001109457.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102927.1 Rattus norvegicus epididymal peptidase inhibitor (Eppin), mRNA.

Price & Availability↑

CloneID	ORa09989
Clone ID Related Accession (Same CDS sequence)	NM_001109457.1
Accession Version	NM_001109457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	eppin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474005.2. On Oct 4, 2007 this sequence version replaced XM_001062609.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689604, SAMN02642705 [ECO:0000348] ##Evidence-Data-END##

ATGAAGTTTT CCAGATTTGT GAGCATCCTC GTGCTATTTG GCCTGCTTAC AAAGGTCCAG 
GGACCTAGTC TAACTGACTT TCTGTTTCCC AGGAGATGCC CCAGATTCAG AGAGGAGTGT 
GAACACAGAG AGAGGGACCT TTGTACCAGG GACAGGGACT GCCAGAAGAG AGAGAAGTGC 
TGTATCTTCA GCTGCGGAAA GAAATGTCTA AACCCCCAAC AAGATATTTG CAGCCTACCA 
AAAGACTCTG GCTACTGCAT GGCTTACTTC CCTCGTTGGT GGTATAATAA GAAAAACGGC 
ACGTGCCAAC TCTTCATCTA TGGTGGTTGC CAGGGAAACA ATAACAACTT CCAGTCCCAA 
AGTATATGCC AGAATGCCTG TGAAAAAAAA AGCAATTCCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102927.1
CDS	60..464
Translation	MKFSRFVSILVLFGLLTKVQGPSLTDFLFPRRCPRFREECEHRERDLCTRDRDCQKREKCCIFSCGKKCLNPQQDICSLPKDSGYCMAYFPRWWYNKKNGTCQLFIYGGCQGNNNNFQSQSICQNACEKKSNST

Target ORF information:

RefSeq Version	NM_001109457.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus epididymal peptidase inhibitor (Eppin), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001109457.1

ORF Insert Sequence:

ATGAAGTTTT CCAGATTTGT GAGCATCCTC GTGCTATTTG GCCTGCTTAC AAAGGTCCAG 
GGACCTAGTC TAACTGACTT TCTGTTTCCC AGGAGATGCC CCAGATTCAG AGAGGAGTGT 
GAACACAGAG AGAGGGACCT TTGTACCAGG GACAGGGACT GCCAGAAGAG AGAGAAGTGC 
TGTATCTTCA GCTGCGGAAA GAAATGTCTA AACCCCCAAC AAGATATTTG CAGCCTACCA 
AAAGACTCTG GCTACTGCAT GGCTTACTTC CCTCGTTGGT GGTATAATAA GAAAAACGGC 
ACGTGCCAAC TCTTCATCTA TGGTGGTTGC CAGGGAAACA ATAACAACTT CCAGTCCCAA 
AGTATATGCC AGAATGCCTG TGAAAAAAAA AGCAATTCCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

De novo assembly of the transcriptome of Acanthaster planci testes.
Molecular ecology resources15(4)953-66(2015 Jul)
Stewart MJ,Stewart P,Rivera-Posada J

Epididymal protease inhibitor (EPPIN) is differentially expressed in the male rat reproductive tract and immunolocalized in maturing spermatozoa.
Molecular reproduction and development79(12)832-42(2012 Dec)
Silva EJ,Patr?o MT,Tsuruta JK,O'Rand MG,Avellar MC

Loss of calcium in human spermatozoa via EPPIN, the semenogelin receptor.
Biology of reproduction86(2)55(2012 Feb)
O'Rand MG,Widgren EE

Identification and characterization of Wfdc gene expression in the male reproductive tract of the rat.
Molecular reproduction and development78(9)633-41(2011 Sep)
Rajesh A,Madhubabu G,Yenugu S

Functional domains of the human epididymal protease inhibitor, eppin.
The FEBS journal275(8)1742-50(2008 Apr)
McCrudden MT,Dafforn TR,Houston DF,Turkington PT,Timson DJ