Mbnl2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mbnl2
Entrez Gene ID	680445
Full Name	muscleblind-like splicing regulator 2
General protein information	Preferred Names muscleblind-like splicing regulator 2 Names muscleblind-like protein 2 muscleblind-like 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001111064.1	NP_001104534.1	muscleblind-like protein 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MBNL2	NP_001075169.1
Homo sapiens (human)	MBNL2	NP_659002.1
Bos taurus (cattle)	MBNL2	NP_001093180.1
Mus musculus (house mouse)	Mbnl2	NP_780550.1
Xenopus tropicalis (tropical clawed frog)	mbnl2	NP_001072767.2
Pan troglodytes (chimpanzee)	MBNL2	XP_001141054.1
Canis lupus familiaris (dog)	MBNL2	XP_005634082.1
Rattus norvegicus (Norway rat)	Mbnl2	NP_001104534.1
Danio rerio (zebrafish)	mbnl2	NP_001155142.1

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

MBNL and CELF proteins regulate alternative splicing of the skeletal muscle chloride channel CLCN1.
Kino Y, Washizu C, Oma Y, Onishi H, Nezu Y, Sasagawa N, Nukina N, Ishiura S
Nucleic acids research37(19)6477-90(2009 Oct)

Muscleblind-like 2: circadian expression in the mammalian pineal gland is controlled by an adrenergic-cAMP mechanism.
Kim JS, Coon SL, Weller JL, Blackshaw S, Rath MF, M?ller M, Klein DC
Journal of neurochemistry110(2)756-64(2009 Jul)

Interaction of muscleblind, CUG-BP1 and hnRNP H proteins in DM1-associated aberrant IR splicing.
Paul S, Dansithong W, Kim D, Rossi J, Webster NJ, Comai L, Reddy S
The EMBO journal25(18)4271-83(2006 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mbnl2 is strongly expressed in the rat pineal gland. Mbnl2 mRNA and protein exhibit a circadian rhythm with ~ sevenfold higher values at night than day levels. Mbnl2 mRNA and protein levels are controlled by an adrenergic/cAMP mechanism.
Title: Muscleblind-like 2: circadian expression in the mammalian pineal gland is controlled by an adrenergic-cAMP mechanism.

The following Mbnl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mbnl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00880	NM_001111064.1 Latest version!	Rattus norvegicus muscleblind-like splicing regulator 2 (Mbnl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mbnl2 ( NM_001111064.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001104534.1 Rattus norvegicus muscleblind-like splicing regulator 2 (Mbnl2), mRNA.

Price & Availability↑

CloneID	ORa00880
Clone ID Related Accession (Same CDS sequence)	NM_001111064.1
Accession Version	NM_001111064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-15
Organism	Rattus norvegicus(Norway rat)
Product	muscleblind-like protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK603100.1, CB757029.1, AA818999.1, BE111982.1, AI602815.1, CN540999.1, CO405101.1, CB576955.1, CV108304.1 and BQ202225.1. On or before Dec 3, 2007 this sequence version replaced XM_001075009.1, XM_001074963.1, XM_001075030.1, XM_001074986.1, XM_001075050.1, XM_001074917.1, XM_001074939.1, XM_001074898.1. Sequence Note: This RefSeq record was created from multiple transcripts because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTTGA ACGTTGCCCC CGTGAGAGAC ACAAAGTGGC TGACGCTGGA AGTCTGCAGA 
CAGTACCAGA GAGGAACGTG CTCACGCTCC GATGAAGAAT GCAAATTCGC TCACCCCCCA 
AAAAGTTGCC AGGTTGAAAA TGGAAGAGTA ATTGCCTGCT TTGATTCCCT CAAGGGCCGC 
TGTTCAAGAG AGAACTGCAA ATATCTTCAT CCTCCAACAC ACCTAAAAAC TCAGCTAGAA 
ATCAATGGGA GGAACAATTT GATCCAGCAA AAAACTGCAG CAGCGATGCT TGCCCAGCAG 
ATGCAATTCA TGTTTCCAGG AACACCGCTC CACCCTGTGC CCACTTTTCC TGTAGGTCCC 
ACCATAGGGA CAAATGCGGC CATTAGCTTT GCTCCTTACT TAGCGCCTGT CACCCCTGGA 
GTGGGGTTAG TCCCAACAGA GGTTCTACCC ACCACACCTG TCATTGTTCC GGGAAGTCCG 
CCGGTCACTG TCCCGGGCTC AACTGCAACT CAGAAACTTC TCAGGACCGA TAAACTGGAG 
GTATGCAGGG AGTTCCAGCG AGGAAACTGT GCCCGGGGAG AGACAGACTG CCGCTTTGCA 
CACCCGGCAG ACAGCACCAT GATCGACACA AACGACAACA CCGTAACCGT TTGTATGGAT 
TACATAAAGG GGCGTTGCAT GAGGGAGAAA TGCAAATATT TTCACCCTCC TGCACACTTG 
CAGGCCAAAA TCAAAGCTGC GCAGCACCAA GCCAACCAGG CCGCGGTGGC CGCCCAGGCA 
GCCGCGGCCG CGGCCACAGT CATGACTCAG TCGACTGCCA AAGCACTGAA GCGACCTCTC 
GAAGCAACTG TAGACCTGGC CTTCCCTCCC GGTGCTCTTC ACCCTTTACC AAAGAGACAA 
GCACTTGAAA AAAGCAACGG AGCCAGCACG GTCTTCAACC CCAGCGTCTT GCACTACCAG 
CAGGCTTTGA CCAGTGCGCA GTTGCAGCAA CACACGGCGT TCATTCCCAC AGTACCCATG 
ATGCACAGCG CTACGTCCGC CACTGTCTCT GCAGCAACAA CTCCTGCAAC AAGTGTCCCC 
TTCGCAGCAA CAGCCACAGC CAATCAGATA ATTCTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001104534.1
CDS	682..1803
Translation	MALNVAPVRDTKWLTLEVCRQYQRGTCSRSDEECKFAHPPKSCQVENGRVIACFDSLKGRCSRENCKYLHPPTHLKTQLEINGRNNLIQQKTAAAMLAQQMQFMFPGTPLHPVPTFPVGPTIGTNAAISFAPYLAPVTPGVGLVPTEVLPTTPVIVPGSPPVTVPGSTATQKLLRTDKLEVCREFQRGNCARGETDCRFAHPADSTMIDTNDNTVTVCMDYIKGRCMREKCKYFHPPAHLQAKIKAAQHQANQAAVAAQAAAAAATVMTQSTAKALKRPLEATVDLAFPPGALHPLPKRQALEKSNGASTVFNPSVLHYQQALTSAQLQQHTAFIPTVPMMHSATSATVSAATTPATSVPFAATATANQIILK

Target ORF information:

RefSeq Version	NM_001111064.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus muscleblind-like splicing regulator 2 (Mbnl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001111064.1

ORF Insert Sequence:

ATGGCCTTGA ACGTTGCCCC CGTGAGAGAC ACAAAGTGGC TGACGCTGGA AGTCTGCAGA 
CAGTACCAGA GAGGAACGTG CTCACGCTCC GATGAAGAAT GCAAATTCGC TCACCCCCCA 
AAAAGTTGCC AGGTTGAAAA TGGAAGAGTA ATTGCCTGCT TTGATTCCCT CAAGGGCCGC 
TGTTCAAGAG AGAACTGCAA ATATCTTCAT CCTCCAACAC ACCTAAAAAC TCAGCTAGAA 
ATCAATGGGA GGAACAATTT GATCCAGCAA AAAACTGCAG CAGCGATGCT TGCCCAGCAG 
ATGCAATTCA TGTTTCCAGG AACACCGCTC CACCCTGTGC CCACTTTTCC TGTAGGTCCC 
ACCATAGGGA CAAATGCGGC CATTAGCTTT GCTCCTTACT TAGCGCCTGT CACCCCTGGA 
GTGGGGTTAG TCCCAACAGA GGTTCTACCC ACCACACCTG TCATTGTTCC GGGAAGTCCG 
CCGGTCACTG TCCCGGGCTC AACTGCAACT CAGAAACTTC TCAGGACCGA TAAACTGGAG 
GTATGCAGGG AGTTCCAGCG AGGAAACTGT GCCCGGGGAG AGACAGACTG CCGCTTTGCA 
CACCCGGCAG ACAGCACCAT GATCGACACA AACGACAACA CCGTAACCGT TTGTATGGAT 
TACATAAAGG GGCGTTGCAT GAGGGAGAAA TGCAAATATT TTCACCCTCC TGCACACTTG 
CAGGCCAAAA TCAAAGCTGC GCAGCACCAA GCCAACCAGG CCGCGGTGGC CGCCCAGGCA 
GCCGCGGCCG CGGCCACAGT CATGACTCAG TCGACTGCCA AAGCACTGAA GCGACCTCTC 
GAAGCAACTG TAGACCTGGC CTTCCCTCCC GGTGCTCTTC ACCCTTTACC AAAGAGACAA 
GCACTTGAAA AAAGCAACGG AGCCAGCACG GTCTTCAACC CCAGCGTCTT GCACTACCAG 
CAGGCTTTGA CCAGTGCGCA GTTGCAGCAA CACACGGCGT TCATTCCCAC AGTACCCATG 
ATGCACAGCG CTACGTCCGC CACTGTCTCT GCAGCAACAA CTCCTGCAAC AAGTGTCCCC 
TTCGCAGCAA CAGCCACAGC CAATCAGATA ATTCTGAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

MBNL and CELF proteins regulate alternative splicing of the skeletal muscle chloride channel CLCN1.
Nucleic acids research37(19)6477-90(2009 Oct)
Kino Y,Washizu C,Oma Y,Onishi H,Nezu Y,Sasagawa N,Nukina N,Ishiura S

Muscleblind-like 2: circadian expression in the mammalian pineal gland is controlled by an adrenergic-cAMP mechanism.
Journal of neurochemistry110(2)756-64(2009 Jul)
Kim JS,Coon SL,Weller JL,Blackshaw S,Rath MF,M?ller M,Klein DC

Interaction of muscleblind, CUG-BP1 and hnRNP H proteins in DM1-associated aberrant IR splicing.
The EMBO journal25(18)4271-83(2006 Sep)
Paul S,Dansithong W,Kim D,Rossi J,Webster NJ,Comai L,Reddy S