Sohlh2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sohlh2
Entrez Gene ID	619575
Full Name	spermatogenesis and oogenesis specific basic helix-loop-helix 2
Synonyms	RGD1304752
General protein information	Preferred Names spermatogenesis and oogenesis specific basic helix-loop-helix 2 Names spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q26

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034961.1	NP_001030133.1	spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SOHLH2	XP_543134.3
Pan troglodytes (chimpanzee)	SOHLH2	XP_003314166.1
	SOHLH2	XP_002800767.1
Mus musculus (house mouse)	Sohlh2	NP_083213.2
Rattus norvegicus (Norway rat)	Sohlh2	NP_001030133.1
Homo sapiens (human)	CCDC169-SOHLH2	NP_001185839.1
Bos taurus (cattle)	SOHLH2	XP_002691848.1

Gene Ontology
Bibliography

Related articles in PubMed

Transcription factors SOHLH1 and SOHLH2 coordinate oocyte differentiation without affecting meiosis I.
Shin YH, Ren Y, Suzuki H, Golnoski KJ, Ahn HW, Mico V, Rajkovic A
The Journal of clinical investigation127(6)2106-2117(2017 Jun)

SOHLH1 and SOHLH2 control Kit expression during postnatal male germ cell development.
Barrios F, Filipponi D, Campolo F, Gori M, Bramucci F, Pellegrini M, Ottolenghi S, Rossi P, Jannini EA, Dolci S
Journal of cell science125(Pt 6)1455-64(2012 Mar)

SOHLH1 and SOHLH2 coordinate spermatogonial differentiation.
Suzuki H, Ahn HW, Chu T, Bowden W, Gassei K, Orwig K, Rajkovic A
Developmental biology361(2)301-12(2012 Jan)

Germ cell-specific transcriptional regulator sohlh2 is essential for early mouse folliculogenesis and oocyte-specific gene expression.
Choi Y, Yuan D, Rajkovic A
Biology of reproduction79(6)1176-82(2008 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sohlh2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sohlh2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09143	NM_001034961.1 Latest version!	Rattus norvegicus spermatogenesis and oogenesis specific basic helix-loop-helix 2 (Sohlh2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$272.30 $389.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sohlh2 ( NM_001034961.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001030133.1 Rattus norvegicus spermatogenesis and oogenesis specific basic helix-loop-helix 2 (Sohlh2), mRNA.

Price & Availability↑

CloneID	ORa09143
Clone ID Related Accession (Same CDS sequence)	NM_001034961.1
Accession Version	NM_001034961.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC104697.1. ##Evidence-Data-START## Transcript exon combination :: BC104697.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689604, SAMN02642714 [ECO:0000348] ##Evidence-Data-END##

ATGGCCGACA GGATCAGCAC TGGGGAGCTA GGCCGGAGGC CGGGGCAGGG AAGAGTAGAC 
CTTTTGCTAG TTGGAGATGC CACACGATAT TTCCTGGCTG GTTCTGTGCA GAAATTCTTT 
TCCAGCACAG CACAGATCAC CCTGACCATT AGCAACGTGA AGAAGGTAGC TGCGCTTCTG 
GCTGCTAACT CGTTTGACAT AATCTTCTTG AAGGTGACTT CTACCTTAAC TGCAGAGGAG 
CAGGAGGCCG TCAGATTGAT TAGATCTGGC AAGAAGAAAA ACACACATTT GCTGTTTGCT 
TTTGTAATAC CTGAAAAACT TAGAGGCTAT ATTTCAGATT ATGGAGCTGA TATTAGTTTC 
AATGAGCCAC TGACCTTGGA AAAAGTGAAC ACTGTGATAA ACTACTGGAA AACGTATTTC 
ACAAATACGG ATATGGGGAA CACTGAGTTG CCTCCAGAAT GCAGACTCTA CTTCCAGACT 
TCCTGCAGCG AACTCGGAGG GCATTTCCCT ACAGATCTGT TTCTTTGCTC TGAACTGCTG 
AACAATGACA CTGGACTTGG ATTAAAGGCC CCGCTGTCGA GTCCAGAGAG AAACAAAAAG 
GCTTCTTTTC TTCACTCAAG CAAAGAAAAG CTGAGACGAG AGCGCATCAA GTTTTGCTGT 
GAGCAGCTGC GCACCCTCCT GCCATACGTC AAGGGGAGGA AGAGTGATGT GGCTTCCGTC 
ATCGAGGCCA CAGTTGATTA TGTGAAGCAG GTCCGGGAGT CTCTCTCGCC AGCCATTATG 
GCCCAGATTA CAGAATCCCT TCAGAGCAAT AAGAGGTTTT CTAAGAGACA GATGCCCATC 
GAGCTGTTCC TCCCCTGCAC AGCCACATCA CAGAGGGGAG ATGCTATGCT GACAAGTGCT 
TTCTCACCTG TGCAAGAGAT CCAGCTCCTG GCTGACCAGG GCTTGAATGT CTATTCCATG 
CCTGCTGCAG GAGGACCTTT GGAGGAAGCT GTGAGAGGTC AGCCGGGCTC TGTTTCAGAG 
GATCTTTATA AGACTCGAGT CCCCAGCACG ACTCTCTCTC TGAACTCCTT CCATGCTGTC 
AGATACTGCT CTGGACCCGT CTCTCCCCAT GAGGCAGCTG CCAGAACAAA CCAGAACATT 
TCCATTTATT TACCACCCAC TGGGCCCAGT GTGTCCAGCT TTACTCCTCA GCACTGCAAT 
GCCATGCTTT GCCCAACACG CCCAGCCAGC TCCAGTTGTC TGTGCACTTC CGGCCATGAG 
CTCCCAGCCA GTTCTCGCAC TGCCTCATCC AGCATCTTTC GTGGGTTCCG AGAATCAGAC 
TCGGGTCACC AGGCTTCTCA GCAACCCACT GGGCCGTCTC TGCAGCCTCA AGACAGCAGT 
TACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030133.1
CDS	33..1421
Translation	MADRISTGELGRRPGQGRVDLLLVGDATRYFLAGSVQKFFSSTAQITLTISNVKKVAALLAANSFDIIFLKVTSTLTAEEQEAVRLIRSGKKKNTHLLFAFVIPEKLRGYISDYGADISFNEPLTLEKVNTVINYWKTYFTNTDMGNTELPPECRLYFQTSCSELGGHFPTDLFLCSELLNNDTGLGLKAPLSSPERNKKASFLHSSKEKLRRERIKFCCEQLRTLLPYVKGRKSDVASVIEATVDYVKQVRESLSPAIMAQITESLQSNKRFSKRQMPIELFLPCTATSQRGDAMLTSAFSPVQEIQLLADQGLNVYSMPAAGGPLEEAVRGQPGSVSEDLYKTRVPSTTLSLNSFHAVRYCSGPVSPHEAAARTNQNISIYLPPTGPSVSSFTPQHCNAMLCPTRPASSSCLCTSGHELPASSRTASSSIFRGFRESDSGHQASQQPTGPSLQPQDSSYF

Target ORF information:

RefSeq Version	NM_001034961.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spermatogenesis and oogenesis specific basic helix-loop-helix 2 (Sohlh2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034961.1

ORF Insert Sequence:

ATGGCCGACA GGATCAGCAC TGGGGAGCTA GGCCGGAGGC CGGGGCAGGG AAGAGTAGAC 
CTTTTGCTAG TTGGAGATGC CACACGATAT TTCCTGGCTG GTTCTGTGCA GAAATTCTTT 
TCCAGCACAG CACAGATCAC CCTGACCATT AGCAACGTGA AGAAGGTAGC TGCGCTTCTG 
GCTGCTAACT CGTTTGACAT AATCTTCTTG AAGGTGACTT CTACCTTAAC TGCAGAGGAG 
CAGGAGGCCG TCAGATTGAT TAGATCTGGC AAGAAGAAAA ACACACATTT GCTGTTTGCT 
TTTGTAATAC CTGAAAAACT TAGAGGCTAT ATTTCAGATT ATGGAGCTGA TATTAGTTTC 
AATGAGCCAC TGACCTTGGA AAAAGTGAAC ACTGTGATAA ACTACTGGAA AACGTATTTC 
ACAAATACGG ATATGGGGAA CACTGAGTTG CCTCCAGAAT GCAGACTCTA CTTCCAGACT 
TCCTGCAGCG AACTCGGAGG GCATTTCCCT ACAGATCTGT TTCTTTGCTC TGAACTGCTG 
AACAATGACA CTGGACTTGG ATTAAAGGCC CCGCTGTCGA GTCCAGAGAG AAACAAAAAG 
GCTTCTTTTC TTCACTCAAG CAAAGAAAAG CTGAGACGAG AGCGCATCAA GTTTTGCTGT 
GAGCAGCTGC GCACCCTCCT GCCATACGTC AAGGGGAGGA AGAGTGATGT GGCTTCCGTC 
ATCGAGGCCA CAGTTGATTA TGTGAAGCAG GTCCGGGAGT CTCTCTCGCC AGCCATTATG 
GCCCAGATTA CAGAATCCCT TCAGAGCAAT AAGAGGTTTT CTAAGAGACA GATGCCCATC 
GAGCTGTTCC TCCCCTGCAC AGCCACATCA CAGAGGGGAG ATGCTATGCT GACAAGTGCT 
TTCTCACCTG TGCAAGAGAT CCAGCTCCTG GCTGACCAGG GCTTGAATGT CTATTCCATG 
CCTGCTGCAG GAGGACCTTT GGAGGAAGCT GTGAGAGGTC AGCCGGGCTC TGTTTCAGAG 
GATCTTTATA AGACTCGAGT CCCCAGCACG ACTCTCTCTC TGAACTCCTT CCATGCTGTC 
AGATACTGCT CTGGACCCGT CTCTCCCCAT GAGGCAGCTG CCAGAACAAA CCAGAACATT 
TCCATTTATT TACCACCCAC TGGGCCCAGT GTGTCCAGCT TTACTCCTCA GCACTGCAAT 
GCCATGCTTT GCCCAACACG CCCAGCCAGC TCCAGTTGTC TGTGCACTTC CGGCCATGAG 
CTCCCAGCCA GTTCTCGCAC TGCCTCATCC AGCATCTTTC GTGGGTTCCG AGAATCAGAC 
TCGGGTCACC AGGCTTCTCA GCAACCCACT GGGCCGTCTC TGCAGCCTCA AGACAGCAGT 
TACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transcription factors SOHLH1 and SOHLH2 coordinate oocyte differentiation without affecting meiosis I.
The Journal of clinical investigation127(6)2106-2117(2017 Jun)
Shin YH,Ren Y,Suzuki H,Golnoski KJ,Ahn HW,Mico V,Rajkovic A

SOHLH1 and SOHLH2 control Kit expression during postnatal male germ cell development.
Journal of cell science125(Pt 6)1455-64(2012 Mar)
Barrios F,Filipponi D,Campolo F,Gori M,Bramucci F,Pellegrini M,Ottolenghi S,Rossi P,Jannini EA,Dolci S

SOHLH1 and SOHLH2 coordinate spermatogonial differentiation.
Developmental biology361(2)301-12(2012 Jan)
Suzuki H,Ahn HW,Chu T,Bowden W,Gassei K,Orwig K,Rajkovic A

Germ cell-specific transcriptional regulator sohlh2 is essential for early mouse folliculogenesis and oocyte-specific gene expression.
Biology of reproduction79(6)1176-82(2008 Dec)
Choi Y,Yuan D,Rajkovic A