Hand1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hand1
Entrez Gene ID	59112
Full Name	heart and neural crest derivatives expressed 1
Synonyms	eHand
General protein information	Preferred Names heart and neural crest derivatives expressed 1 Names heart- and neural crest derivatives-expressed protein 1 extraembryonic tissues, heart, autonomic nervous system and neural crest derivatives-expressed protein 1 heart and neural crest derivatives expressed transcript 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q22
Summary	basic Helix-Loop-Helix (bHLH) transcription factor; essential for normal cardiac and extraembryonic development [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021592.2	NP_067603.1	heart- and neural crest derivatives-expressed protein 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HAND1	NP_004812.1
Bos taurus (cattle)	HAND1	NP_001069229.1
Mus musculus (house mouse)	Hand1	NP_032239.1
Gallus gallus (chicken)	HAND1	NP_990296.1
	HAND1	XP_001111722.1
Xenopus tropicalis (tropical clawed frog)	hand1	NP_001016743.1
Pan troglodytes (chimpanzee)	HAND1	XP_518050.2
Rattus norvegicus (Norway rat)	Hand1	NP_067603.1
Drosophila melanogaster (fruit fly)	Hand	NP_609370.2

Gene Ontology
Bibliography

Related articles in PubMed

The basic helix-loop-helix transcription factor Hand1 regulates mouse development as a homodimer.
Hu D, Scott IC, Snider F, Geary-Joo C, Zhao X, Simmons DG, Cross JC
Developmental biology382(2)470-81(2013 Oct)

A functional genetic study identifies HAND1 mutations in septation defects of the human heart.
Reamon-Buettner SM, Ciribilli Y, Traverso I, Kuhls B, Inga A, Borlak J
Human molecular genetics18(19)3567-78(2009 Oct)

FGF8 regulates myogenesis and induces Runx2 expression and osteoblast differentiation in cultured cells.
Omoteyama K, Takagi M
Journal of cellular biochemistry106(4)546-52(2009 Mar)

Hand2 controls osteoblast differentiation in the branchial arch by inhibiting DNA binding of Runx2.
Funato N, Chapman SL, McKee MD, Funato H, Morris JA, Shelton JM, Richardson JA, Yanagisawa H
Development (Cambridge, England)136(4)615-25(2009 Feb)

A loss-of-function mutation in the binding domain of HAND1 predicts hypoplasia of the human hearts.
Reamon-Buettner SM, Ciribilli Y, Inga A, Borlak J
Human molecular genetics17(10)1397-405(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Foxc2 is directly involved in the regulation of Hand1 expression.
Title: FGF8 regulates myogenesis and induces Runx2 expression and osteoblast differentiation in cultured cells.

MEF2/HAND1 interaction results in synergistic activation of MEF2-dependent promoters, and MEF2 binding sites are sufficient to mediate this synergy
Title: MEF2-dependent recruitment of the HAND1 transcription factor results in synergistic activation of target promoters.

expressed in the adult rodent heart and is modulated during cardiac hypertrophy
Title: HAND1 and HAND2 are expressed in the adult-rodent heart and are modulated during cardiac hypertrophy.

The following Hand1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hand1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04914	NM_021592.2 Latest version!	Rattus norvegicus heart and neural crest derivatives expressed 1 (Hand1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hand1 ( NM_021592.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_067603.1 Rattus norvegicus heart and neural crest derivatives expressed 1 (Hand1), mRNA.

Price & Availability↑

CloneID	ORa04914
Clone ID Related Accession (Same CDS sequence)	NM_021592.2
Accession Version	NM_021592.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	heart- and neural crest derivatives-expressed protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC097324.1. On Jun 12, 2005 this sequence version replaced NM_021592.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC097324.1, Y08140.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689604, SAMN01906347 [ECO:0000348] ##Evidence-Data-END##

ATGAACCTCG TGGGCAGCTA CGCACACCAT CACCACCATC ACCACTCACA CCCGCCGCAC 
CCCATGCTGC ACGAACCCTT CCTCTTTGGT CCGGCCTCGC GTTGCCACCA GGAGCGGCCT 
TACTTCCAGA GCTGGCTGCT GAGCCCGGCT GATGCTGCCC CAGATTTCCC TGCCGGCGGG 
CCACCACCTA CCACCGCAGT AGCAGCGGCT GCCTATGGTC CCGATGCCAG GCCGAGTCAG 
AGCCCAGGTC GGCTGGAGGC TCTTGGAGGC CGCCTGCCCA GGCGAAAAGG TTCAGGACCC 
AAAAAGGAGC GGAGGCGCAC AGAGAGCATT AACAGTGCGT TCGCGGAGCT GCGTGAGTGC 
ATCCCCAATG TGCCTGCGGA CACCAAGCTC TCCAAGATCA AGACTCTGCG CCTGGCCACC 
AGCTACATCG CCTACTTGAT GGACGTGCTG GCCAAGGATG CACAAGCAGG TGACCCCGAG 
GCCTTCAAGG CTGAACTAAA AAAGACGGAT GGCGGTCGCG AAAGCAAGCG GAAAAGGGAG 
TCGCCTCAGC AGCACGAAGG CCTTCCTCCT GCCCTGGGGC CAAGCGAGAA GAGGATTAAA 
GGACGCACTG GCTGGCCTCA GCAAGTCTGG GCGCTGGAGC TAAATCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067603.1
CDS	211..861
Translation	MNLVGSYAHHHHHHHSHPPHPMLHEPFLFGPASRCHQERPYFQSWLLSPADAAPDFPAGGPPPTTAVAAAAYGPDARPSQSPGRLEALGGRLPRRKGSGPKKERRRTESINSAFAELRECIPNVPADTKLSKIKTLRLATSYIAYLMDVLAKDAQAGDPEAFKAELKKTDGGRESKRKRESPQQHEGLPPALGPSEKRIKGRTGWPQQVWALELNQ

Target ORF information:

RefSeq Version	NM_021592.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus heart and neural crest derivatives expressed 1 (Hand1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021592.2

ORF Insert Sequence:

ATGAACCTCG TGGGCAGCTA CGCACACCAT CACCACCATC ACCACTCACA CCCGCCGCAC 
CCCATGCTGC ACGAACCCTT CCTCTTTGGT CCGGCCTCGC GTTGCCACCA GGAGCGGCCT 
TACTTCCAGA GCTGGCTGCT GAGCCCGGCT GATGCTGCCC CAGATTTCCC TGCCGGCGGG 
CCACCACCTA CCACCGCAGT AGCAGCGGCT GCCTATGGTC CCGATGCCAG GCCGAGTCAG 
AGCCCAGGTC GGCTGGAGGC TCTTGGAGGC CGCCTGCCCA GGCGAAAAGG TTCAGGACCC 
AAAAAGGAGC GGAGGCGCAC AGAGAGCATT AACAGTGCGT TCGCGGAGCT GCGTGAGTGC 
ATCCCCAATG TGCCTGCGGA CACCAAGCTC TCCAAGATCA AGACTCTGCG CCTGGCCACC 
AGCTACATCG CCTACTTGAT GGACGTGCTG GCCAAGGATG CACAAGCAGG TGACCCCGAG 
GCCTTCAAGG CTGAACTAAA AAAGACGGAT GGCGGTCGCG AAAGCAAGCG GAAAAGGGAG 
TCGCCTCAGC AGCACGAAGG CCTTCCTCCT GCCCTGGGGC CAAGCGAGAA GAGGATTAAA 
GGACGCACTG GCTGGCCTCA GCAAGTCTGG GCGCTGGAGC TAAATCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The basic helix-loop-helix transcription factor Hand1 regulates mouse development as a homodimer.
Developmental biology382(2)470-81(2013 Oct)
Hu D,Scott IC,Snider F,Geary-Joo C,Zhao X,Simmons DG,Cross JC

A functional genetic study identifies HAND1 mutations in septation defects of the human heart.
Human molecular genetics18(19)3567-78(2009 Oct)
Reamon-Buettner SM,Ciribilli Y,Traverso I,Kuhls B,Inga A,Borlak J

FGF8 regulates myogenesis and induces Runx2 expression and osteoblast differentiation in cultured cells.
Journal of cellular biochemistry106(4)546-52(2009 Mar)
Omoteyama K,Takagi M

Hand2 controls osteoblast differentiation in the branchial arch by inhibiting DNA binding of Runx2.
Development (Cambridge, England)136(4)615-25(2009 Feb)
Funato N,Chapman SL,McKee MD,Funato H,Morris JA,Shelton JM,Richardson JA,Yanagisawa H

A loss-of-function mutation in the binding domain of HAND1 predicts hypoplasia of the human hearts.
Human molecular genetics17(10)1397-405(2008 May)
Reamon-Buettner SM,Ciribilli Y,Inga A,Borlak J