Ppp1r1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp1r1a
Entrez Gene ID	58977
Full Name	protein phosphatase 1, regulatory (inhibitor) subunit 1A
General protein information	Preferred Names protein phosphatase 1, regulatory (inhibitor) subunit 1A Names protein phosphatase 1 regulatory subunit 1A I-1 IPP-1 PP1 inhibitor 1 protein phosphatase inhibitor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q36
Summary	inhibits the activity of type 1 protein phosphatase [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022676.3	NP_073167.1	protein phosphatase 1 regulatory subunit 1A
XM_006242437.3	XP_006242499.1	protein phosphatase 1 regulatory subunit 1A isoform X1

Pathways from BioSystems

Homologies

	PPP1R1A	XP_001110247.1
Pan troglodytes (chimpanzee)	PPP1R1A	XP_001156163.2
Homo sapiens (human)	PPP1R1A	NP_006732.3
Mus musculus (house mouse)	Ppp1r1a	NP_067366.1
Rattus norvegicus (Norway rat)	Ppp1r1a	NP_073167.1

Gene Ontology
Bibliography

Related articles in PubMed

Potential of protein phosphatase inhibitor 1 as biomarker of pancreatic ?-cell injury in vitro and in vivo.
Jiang L, Brackeva B, Ling Z, Kramer G, Aerts JM, Schuit F, Keymeulen B, Pipeleers D, Gorus F, Martens GA
Diabetes62(8)2683-8(2013 Aug)

Protein phosphatase inhibitor-1 augments a protein kinase A-dependent increase in the Ca2+ loading of the sarcoplasmic reticulum without changing its Ca2+ release.
Kawashima H, Satoh H, Saotome M, Urushida T, Katoh H, Hayashi H
Circulation journal : official journal of the Japanese Circulation Society73(6)1133-40(2009 Jun)

Role of calcineurin and protein phosphatase-2A in the regulation of phosphatase inhibitor-1 in cardiac myocytes.
El-Armouche A, Bednorz A, Pamminger T, Ditz D, Didi? M, Dobrev D, Eschenhagen T
Biochemical and biophysical research communications346(3)700-6(2006 Aug)

Detection and quantification of protein phosphatase inhibitor-1 gene expression in total rat liver and isolated hepatocytes.
Aleem EA, Flohr T, Hunziker A, Mayer D, Bannasch P, Thielmann HW
Molecular and cellular biochemistry217(1-2)1-12(2001 Jan)

Molecular cloning of protein phosphatase inhibitor-1 and its expression in rat and rabbit tissues.
Elbrecht A, DiRenzo J, Smith RG, Shenolikar S
The Journal of biological chemistry265(23)13415-8(1990 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Protein phosphatase inhibitor-1 augments a protein kinase A-dependent increase in the Ca2+ loading of the sarcoplasmic reticulum without changing its Ca2+ release.
Title: Protein phosphatase inhibitor-1 augments a protein kinase A-dependent increase in the Ca2+ loading of the sarcoplasmic reticulum without changing its Ca2+ release.

calcineurin and protein phosphatase-2A act to maintain a low basal level of phosphorylated (active) phosphatase inhibitor-1 in living cardiac myocytes
Title: Role of calcineurin and protein phosphatase-2A in the regulation of phosphatase inhibitor-1 in cardiac myocytes.

The following Ppp1r1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp1r1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06238	NM_022676.3 Latest version!	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 1A (Ppp1r1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa30793	XM_006242437.3 Latest version!	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 1A (Ppp1r1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp1r1a ( NM_022676.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_073167.1 Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 1A (Ppp1r1a), mRNA.

Price & Availability↑

CloneID	ORa06238
Clone ID Related Accession (Same CDS sequence)	NM_022676.3
Accession Version	NM_022676.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1 regulatory subunit 1A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY648296.1. On Aug 15, 2004 this sequence version replaced NM_022676.2. ##Evidence-Data-START## Transcript exon combination :: AY648296.1, BC078820.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCCCG ACAACAGTCC ACGGAAGATC CAGTTTACGG TTCCGCTGCT GGAGCCTCAC 
CTGGACCCGG AGGCAGCCGA GCAGATTCGG AGGCGCCGCC CCACCCCTGC CACACTTGTG 
CTGACCAGCG ACCAGTCATC CCCAGAAGTA GATGAAGACC GGATCCCCAA CCCACTTCTC 
AAGTCCACAC TGTCAATGTC TCCACGGCAA CGGAAGAAGA TGACAAGGAC TACACCCACC 
ATGAAAGAGC TCCAGACAAT GGTTGAACAT CACCTAGGGC AACAGAAACA AGGGGAAGAA 
CCTGAGGGAG CCACTGAGAG CACAGGGAAC CAGGAGTCCT GCCCACCTGG GATCCCAGAC 
ACAGGCTCAG CGTCAAGGCC AGATACCTCG GGGACAGCAC AAAAGCCTGC AGAATCCAAA 
CCCAAGACTC AGGAGCAGCG TGGTGTGGAG CCCAGCACAG AGGACCTTTC AGCCCACATG 
CTACCACTGG ATTCCCAAGG AGCCAGCTTG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073167.1
CDS	135..650
Translation	MEPDNSPRKIQFTVPLLEPHLDPEAAEQIRRRRPTPATLVLTSDQSSPEVDEDRIPNPLLKSTLSMSPRQRKKMTRTTPTMKELQTMVEHHLGQQKQGEEPEGATESTGNQESCPPGIPDTGSASRPDTSGTAQKPAESKPKTQEQRGVEPSTEDLSAHMLPLDSQGASLV

Target ORF information:

RefSeq Version	NM_022676.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 1A (Ppp1r1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022676.3

ORF Insert Sequence:

ATGGAGCCCG ACAACAGTCC ACGGAAGATC CAGTTTACGG TTCCGCTGCT GGAGCCTCAC 
CTGGACCCGG AGGCAGCCGA GCAGATTCGG AGGCGCCGCC CCACCCCTGC CACACTTGTG 
CTGACCAGCG ACCAGTCATC CCCAGAAGTA GATGAAGACC GGATCCCCAA CCCACTTCTC 
AAGTCCACAC TGTCAATGTC TCCACGGCAA CGGAAGAAGA TGACAAGGAC TACACCCACC 
ATGAAAGAGC TCCAGACAAT GGTTGAACAT CACCTAGGGC AACAGAAACA AGGGGAAGAA 
CCTGAGGGAG CCACTGAGAG CACAGGGAAC CAGGAGTCCT GCCCACCTGG GATCCCAGAC 
ACAGGCTCAG CGTCAAGGCC AGATACCTCG GGGACAGCAC AAAAGCCTGC AGAATCCAAA 
CCCAAGACTC AGGAGCAGCG TGGTGTGGAG CCCAGCACAG AGGACCTTTC AGCCCACATG 
CTACCACTGG ATTCCCAAGG AGCCAGCTTG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r1a ( XM_006242437.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242499.1 Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 1A (Ppp1r1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30793
Clone ID Related Accession (Same CDS sequence)	XM_006242437.3
Accession Version	XM_006242437.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1 regulatory subunit 1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242437.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCCG ACAACAGTCC ACGGAAGATC CAGTTTACGG TTCCGCTGCT GGAGCCTCAC 
CTGGACCCGG AGGCAGCCGA GCAGATTCGG AGGCGCCGCC CCACCCCTGC CACACTTGTG 
CTGACCAGCG ACCAGTCATC CCCAGTAGAT GAAGACCGGA TCCCCAACCC ACTTCTCAAG 
TCCACACTGT CAATGTCTCC ACGGCAACGG AAGAAGATGA CAAGGACTAC ACCCACCATG 
AAAGAGCTCC AGACAATGGT TGAACATCAC CTAGGGCAAC AGAAACAAGG GGAAGAACCT 
GAGGGAGCCA CTGAGAGCAC AGGGAACCAG GAGTCCTGCC CACCTGGGAT CCCAGACACA 
GGCTCAGCGT CAAGGCCAGA TACCTCGGGG ACAGCACAAA AGCCTGCAGA ATCCAAACCC 
AAGACTCAGG AGCAGCGTGG TGTGGAGCCC AGCACAGAGG ACCTTTCAGC CCACATGCTA 
CCACTGGATT CCCAAGGAGC CAGCTTGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242499.1
CDS	109..621
Translation	MEPDNSPRKIQFTVPLLEPHLDPEAAEQIRRRRPTPATLVLTSDQSSPVDEDRIPNPLLKSTLSMSPRQRKKMTRTTPTMKELQTMVEHHLGQQKQGEEPEGATESTGNQESCPPGIPDTGSASRPDTSGTAQKPAESKPKTQEQRGVEPSTEDLSAHMLPLDSQGASLV

Target ORF information:

RefSeq Version	XM_006242437.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 1A (Ppp1r1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242437.3

ORF Insert Sequence:

ATGGAGCCCG ACAACAGTCC ACGGAAGATC CAGTTTACGG TTCCGCTGCT GGAGCCTCAC 
CTGGACCCGG AGGCAGCCGA GCAGATTCGG AGGCGCCGCC CCACCCCTGC CACACTTGTG 
CTGACCAGCG ACCAGTCATC CCCAGTAGAT GAAGACCGGA TCCCCAACCC ACTTCTCAAG 
TCCACACTGT CAATGTCTCC ACGGCAACGG AAGAAGATGA CAAGGACTAC ACCCACCATG 
AAAGAGCTCC AGACAATGGT TGAACATCAC CTAGGGCAAC AGAAACAAGG GGAAGAACCT 
GAGGGAGCCA CTGAGAGCAC AGGGAACCAG GAGTCCTGCC CACCTGGGAT CCCAGACACA 
GGCTCAGCGT CAAGGCCAGA TACCTCGGGG ACAGCACAAA AGCCTGCAGA ATCCAAACCC 
AAGACTCAGG AGCAGCGTGG TGTGGAGCCC AGCACAGAGG ACCTTTCAGC CCACATGCTA 
CCACTGGATT CCCAAGGAGC CAGCTTGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Potential of protein phosphatase inhibitor 1 as biomarker of pancreatic ?-cell injury in vitro and in vivo.
Diabetes62(8)2683-8(2013 Aug)
Jiang L,Brackeva B,Ling Z,Kramer G,Aerts JM,Schuit F,Keymeulen B,Pipeleers D,Gorus F,Martens GA

Protein phosphatase inhibitor-1 augments a protein kinase A-dependent increase in the Ca2+ loading of the sarcoplasmic reticulum without changing its Ca2+ release.
Circulation journal : official journal of the Japanese Circulation Society73(6)1133-40(2009 Jun)
Kawashima H,Satoh H,Saotome M,Urushida T,Katoh H,Hayashi H

Role of calcineurin and protein phosphatase-2A in the regulation of phosphatase inhibitor-1 in cardiac myocytes.
Biochemical and biophysical research communications346(3)700-6(2006 Aug)
El-Armouche A,Bednorz A,Pamminger T,Ditz D,Didi? M,Dobrev D,Eschenhagen T

Detection and quantification of protein phosphatase inhibitor-1 gene expression in total rat liver and isolated hepatocytes.
Molecular and cellular biochemistry217(1-2)1-12(2001 Jan)
Aleem EA,Flohr T,Hunziker A,Mayer D,Bannasch P,Thielmann HW

Molecular cloning of protein phosphatase inhibitor-1 and its expression in rat and rabbit tissues.
The Journal of biological chemistry265(23)13415-8(1990 Aug)
Elbrecht A,DiRenzo J,Smith RG,Shenolikar S