St8sia5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	St8sia5
Entrez Gene ID	364901
Full Name	ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5
Synonyms	SIAT8E
General protein information	Preferred Names ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 Names alpha-2,8-sialyltransferase 8E ST8 alpha-N-acetylneuraminate alpha-2,8-sialyltransferase 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18q12.3
Summary	mouse homolog plays a role in ganglioside biosynthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_213628.1	NP_998793.1	alpha-2,8-sialyltransferase 8E
XM_006254968.3	XP_006255030.1	alpha-2,8-sialyltransferase 8E isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ST8SIA5	NP_001009087.1
Danio rerio (zebrafish)	st8sia5	NP_001191083.1
Xenopus tropicalis (tropical clawed frog)	st8sia5	NP_001090748.1
	ST8SIA5	NP_001077419.1
Canis lupus familiaris (dog)	ST8SIA5	XP_003434992.2
Homo sapiens (human)	ST8SIA5	NP_037437.2
Gallus gallus (chicken)	ST8SIA5	XP_004949086.1
Bos taurus (cattle)	ST8SIA5	NP_001179803.1
Mus musculus (house mouse)	St8sia5	NP_038694.2
Rattus norvegicus (Norway rat)	St8sia5	NP_998793.1

Gene Ontology
Bibliography

Related articles in PubMed

Night/day changes in pineal expression of >600 genes: central role of adrenergic/cAMP signaling.
Bailey MJ, Coon SL, Carter DA, Humphries A, Kim JS, Shi Q, Gaildrat P, Morin F, Ganguly S, Hogenesch JB, Weller JL, Rath MF, M?ller M, Baler R, Sugden D, Rangel ZG, Munson PJ, Klein DC
The Journal of biological chemistry284(12)7606-22(2009 Mar)

The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach.
Harduin-Lepers A, Mollicone R, Delannoy P, Oriol R
Glycobiology15(8)805-17(2005 Aug)

Molecular cloning and expression of a fifth type of alpha2,8-sialyltransferase (ST8Sia V). Its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3.
Kono M, Yoshida Y, Kojima N, Tsuji S
The Journal of biological chemistry271(46)29366-71(1996 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression of St8sia5 increases over 15 fold at night in the rat pineal gland.
Title: Night/day changes in pineal expression of >600 genes: central role of adrenergic/cAMP signaling.

The following St8sia5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the St8sia5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05877	NM_213628.1 Latest version!	Rattus norvegicus ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (St8sia5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa38527	XM_006254968.3 Latest version!	Rattus norvegicus ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (St8sia5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

St8sia5 ( NM_213628.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_998793.1 Rattus norvegicus ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (St8sia5), mRNA.

Price & Availability↑

CloneID	ORa05877
Clone ID Related Accession (Same CDS sequence)	NM_213628.1
Accession Version	NM_213628.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-31
Organism	Rattus norvegicus(Norway rat)
Product	alpha-2,8-sialyltransferase 8E
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ699422.1. On Aug 30, 2004 this sequence version replaced XM_344702.1. ##Evidence-Data-START## Transcript exon combination :: AJ699422.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCGCTACG CAGACCCCTC GGCCAACAGG GATTTGTTGG GGAACCGAAC TTTGCTCTTC 
ATCTTCATCT GCGCCTTCGC CTTGGTGACC TTGCTCCAGC AGATCCTGTA CAGCAAGAGC 
TACATCAAGA GGTACTTCGA ATTTTACAAG GAACCTTTAG AATTTAACTC CACGAGATGC 
CTGGAGCTGA GGCAGGAGAT CCTGGAGGTG AAGGTGCTGT CCATGGTGAA GCAGTCTGAA 
CTGTTCGAAA GGTGGAAGAG CCTCCAGATA TGCAAATGGG CGATGGACGC CTCGGAAGCC 
AGCCTCTTCA AGTCCACCTT GTCCAGGTGT TGCAATGCCC CCAACTTTCT CTTCACCACC 
CAGAAGAACA CCCCGGTTGA GACGAATCTC AGGTATGAGG TGGAGTCCAG TGGCTTGTAT 
CACATCGACC AAGAGATCTT CAAAATGTTT CCCAAGGAAA TGCCCTACTA CCGCTCTCAG 
TTTAAGAAGT GTGCTGTGGT GGGCAATGGC GGCATCTTGA AGAACAGCGG CTGCGGGAAG 
GAGATCAACA GCGCTGACTT CGTCTTCCGG TGCAATCTGC CCCCTATCTC AGGGATATAT 
ACCACAGATG TGGGAGAGAA GACAGATGTG GTCACCGTGA ATCCCAGCAT CATAATTGAC 
AGGTTCCACA AGTTGGAGAA GTGGCGGCGG CCCTTCTTCA GCGTGCTGCA GAGGTACGAG 
AACGCCTCGG TGCTGCTGCC GGCCTTCTAC AATGTGCGCA ACACCCTCGT GTCCTTCCGA 
GTCAAGTACA TGCTGGACGA CTTCCAGTCG GGGGAGCCCG TCTACTTCTT CCATCCTCAT 
TACCTGAGCA GCGTGTCGCG CTACTGGCTC AGCCTGGGCG TGCGCGCACG GCGCATCAGC 
ACCGGCCTCA TCCTGGTCAC TGCGGCCCTG GAGCTCTGCG AGGAGGTGCA CCTGTTCGGT 
TTCTGGGCCT TCCCCATGAA CCCCTCCGGC TTCTTCATCA CGCACCACTA CTATGACAAT 
GTCAAGCCCA AGCCCGGCTT TCATGCTATG CCCTCGGAGA TCTTCACCTT CCTGCGCATG 
CACAGCCGCG GCATCCTCCG CGTGCACACA GGCACCTGCA ATTGCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998793.1
CDS	1..1131
Translation	MRYADPSANRDLLGNRTLLFIFICAFALVTLLQQILYSKSYIKRYFEFYKEPLEFNSTRCLELRQEILEVKVLSMVKQSELFERWKSLQICKWAMDASEASLFKSTLSRCCNAPNFLFTTQKNTPVETNLRYEVESSGLYHIDQEIFKMFPKEMPYYRSQFKKCAVVGNGGILKNSGCGKEINSADFVFRCNLPPISGIYTTDVGEKTDVVTVNPSIIIDRFHKLEKWRRPFFSVLQRYENASVLLPAFYNVRNTLVSFRVKYMLDDFQSGEPVYFFHPHYLSSVSRYWLSLGVRARRISTGLILVTAALELCEEVHLFGFWAFPMNPSGFFITHHYYDNVKPKPGFHAMPSEIFTFLRMHSRGILRVHTGTCNCC

Target ORF information:

RefSeq Version	NM_213628.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (St8sia5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213628.1

ORF Insert Sequence:

ATGCGCTACG CAGACCCCTC GGCCAACAGG GATTTGTTGG GGAACCGAAC TTTGCTCTTC 
ATCTTCATCT GCGCCTTCGC CTTGGTGACC TTGCTCCAGC AGATCCTGTA CAGCAAGAGC 
TACATCAAGA GGTACTTCGA ATTTTACAAG GAACCTTTAG AATTTAACTC CACGAGATGC 
CTGGAGCTGA GGCAGGAGAT CCTGGAGGTG AAGGTGCTGT CCATGGTGAA GCAGTCTGAA 
CTGTTCGAAA GGTGGAAGAG CCTCCAGATA TGCAAATGGG CGATGGACGC CTCGGAAGCC 
AGCCTCTTCA AGTCCACCTT GTCCAGGTGT TGCAATGCCC CCAACTTTCT CTTCACCACC 
CAGAAGAACA CCCCGGTTGA GACGAATCTC AGGTATGAGG TGGAGTCCAG TGGCTTGTAT 
CACATCGACC AAGAGATCTT CAAAATGTTT CCCAAGGAAA TGCCCTACTA CCGCTCTCAG 
TTTAAGAAGT GTGCTGTGGT GGGCAATGGC GGCATCTTGA AGAACAGCGG CTGCGGGAAG 
GAGATCAACA GCGCTGACTT CGTCTTCCGG TGCAATCTGC CCCCTATCTC AGGGATATAT 
ACCACAGATG TGGGAGAGAA GACAGATGTG GTCACCGTGA ATCCCAGCAT CATAATTGAC 
AGGTTCCACA AGTTGGAGAA GTGGCGGCGG CCCTTCTTCA GCGTGCTGCA GAGGTACGAG 
AACGCCTCGG TGCTGCTGCC GGCCTTCTAC AATGTGCGCA ACACCCTCGT GTCCTTCCGA 
GTCAAGTACA TGCTGGACGA CTTCCAGTCG GGGGAGCCCG TCTACTTCTT CCATCCTCAT 
TACCTGAGCA GCGTGTCGCG CTACTGGCTC AGCCTGGGCG TGCGCGCACG GCGCATCAGC 
ACCGGCCTCA TCCTGGTCAC TGCGGCCCTG GAGCTCTGCG AGGAGGTGCA CCTGTTCGGT 
TTCTGGGCCT TCCCCATGAA CCCCTCCGGC TTCTTCATCA CGCACCACTA CTATGACAAT 
GTCAAGCCCA AGCCCGGCTT TCATGCTATG CCCTCGGAGA TCTTCACCTT CCTGCGCATG 
CACAGCCGCG GCATCCTCCG CGTGCACACA GGCACCTGCA ATTGCTGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

St8sia5 ( XM_006254968.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255030.1 Rattus norvegicus ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (St8sia5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38527
Clone ID Related Accession (Same CDS sequence)	XM_006254968.3
Accession Version	XM_006254968.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-2,8-sialyltransferase 8E isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254968.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTACG CAGACCCCTC GGCCAACAGG GATTTGTTGG GGAACCGAAC TTTGCTCTTC 
ATCTTCATCT GCGCCTTCGC CTTGGTGACC TTGCTCCAGC AGATCCTGTA CAGCAAGAGC 
TACATCAAGA GAGGCTTCCA GTTTGGTTGG CAGAGGGGCG ACCAACAGGC CAATTGGACT 
GGGCTCTTTA ATGCCAGCGG CAGCCTAACA GAGCAGAACA TCAGTGACTC CAGCTCCAGG 
TACTTCGAAT TTTACAAGGA ACCTTTAGAA TTTAACTCCA CGAGATGCCT GGAGCTGAGG 
CAGGAGATCC TGGAGGTGAA GGTGCTGTCC ATGGTGAAGC AGTCTGAACT GTTCGAAAGG 
TGGAAGAGCC TCCAGATATG CAAATGGGCG ATGGACGCCT CGGAAGCCAG CCTCTTCAAG 
TCCACCTTGT CCAGGTGTTG CAATGCCCCC AACTTTCTCT TCACCACCCA GAAGAACACC 
CCGGTTGAGA CGAATCTCAG GTATGAGGTG GAGTCCAGTG GCTTGTATCA CATCGACCAA 
GAGATCTTCA AAATGTTTCC CAAGGAAATG CCCTACTACC GCTCTCAGTT TAAGAAGTGT 
GCTGTGGTGG GCAATGGCGG CATCTTGAAG AACAGCGGCT GCGGGAAGGA GATCAACAGC 
GCTGACTTCG TCTTCCGGTG CAATCTGCCC CCTATCTCAG GGATATATAC CACAGATGTG 
GGAGAGAAGA CAGATGTGGT CACCGTGAAT CCCAGCATCA TAATTGACAG GTTCCACAAG 
TTGGAGAAGT GGCGGCGGCC CTTCTTCAGC GTGCTGCAGA GGTACGAGAA CGCCTCGGTG 
CTGCTGCCGG CCTTCTACAA TGTGCGCAAC ACCCTCGTGT CCTTCCGAGT CAAGTACATG 
CTGGACGACT TCCAGTCGCG GCAGCCCGTC TACTTCTTCC ATCCTCAGTA CCTGAGCAGC 
GTGTCGCGCT ACTGGCTCAG CCTGGGCGTG CGCGCACGGC GCATCAGCAC CGGCCTCATC 
CTGGTCACTG CGGCCCTGGA GCTCTGCGAG GAGGTGCACC TGTTCGGTTT CTGGGCCTTC 
CCCATGAACC CCTCCGGCTT CTTCATCACG CACCACTACT ATGACAATGT CAAGCCCAAG 
CCCGGCTTTC ATGCTATGCC CTCGGAGATC TTCACCTTCC TGCGCATGCA CAGCCGCGGC 
ATCCTCCGCG TGCACACAGG CACCTGCAAT TGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255030.1
CDS	303..1541
Translation	MRYADPSANRDLLGNRTLLFIFICAFALVTLLQQILYSKSYIKRGFQFGWQRGDQQANWTGLFNASGSLTEQNISDSSSRYFEFYKEPLEFNSTRCLELRQEILEVKVLSMVKQSELFERWKSLQICKWAMDASEASLFKSTLSRCCNAPNFLFTTQKNTPVETNLRYEVESSGLYHIDQEIFKMFPKEMPYYRSQFKKCAVVGNGGILKNSGCGKEINSADFVFRCNLPPISGIYTTDVGEKTDVVTVNPSIIIDRFHKLEKWRRPFFSVLQRYENASVLLPAFYNVRNTLVSFRVKYMLDDFQSRQPVYFFHPQYLSSVSRYWLSLGVRARRISTGLILVTAALELCEEVHLFGFWAFPMNPSGFFITHHYYDNVKPKPGFHAMPSEIFTFLRMHSRGILRVHTGTCNCC

Target ORF information:

RefSeq Version	XM_006254968.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 5 (St8sia5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254968.3

ORF Insert Sequence:

ATGCGCTACG CAGACCCCTC GGCCAACAGG GATTTGTTGG GGAACCGAAC TTTGCTCTTC 
ATCTTCATCT GCGCCTTCGC CTTGGTGACC TTGCTCCAGC AGATCCTGTA CAGCAAGAGC 
TACATCAAGA GAGGCTTCCA GTTTGGTTGG CAGAGGGGCG ACCAACAGGC CAATTGGACT 
GGGCTCTTTA ATGCCAGCGG CAGCCTAACA GAGCAGAACA TCAGTGACTC CAGCTCCAGG 
TACTTCGAAT TTTACAAGGA ACCTTTAGAA TTTAACTCCA CGAGATGCCT GGAGCTGAGG 
CAGGAGATCC TGGAGGTGAA GGTGCTGTCC ATGGTGAAGC AGTCTGAACT GTTCGAAAGG 
TGGAAGAGCC TCCAGATATG CAAATGGGCG ATGGACGCCT CGGAAGCCAG CCTCTTCAAG 
TCCACCTTGT CCAGGTGTTG CAATGCCCCC AACTTTCTCT TCACCACCCA GAAGAACACC 
CCGGTTGAGA CGAATCTCAG GTATGAGGTG GAGTCCAGTG GCTTGTATCA CATCGACCAA 
GAGATCTTCA AAATGTTTCC CAAGGAAATG CCCTACTACC GCTCTCAGTT TAAGAAGTGT 
GCTGTGGTGG GCAATGGCGG CATCTTGAAG AACAGCGGCT GCGGGAAGGA GATCAACAGC 
GCTGACTTCG TCTTCCGGTG CAATCTGCCC CCTATCTCAG GGATATATAC CACAGATGTG 
GGAGAGAAGA CAGATGTGGT CACCGTGAAT CCCAGCATCA TAATTGACAG GTTCCACAAG 
TTGGAGAAGT GGCGGCGGCC CTTCTTCAGC GTGCTGCAGA GGTACGAGAA CGCCTCGGTG 
CTGCTGCCGG CCTTCTACAA TGTGCGCAAC ACCCTCGTGT CCTTCCGAGT CAAGTACATG 
CTGGACGACT TCCAGTCGCG GCAGCCCGTC TACTTCTTCC ATCCTCAGTA CCTGAGCAGC 
GTGTCGCGCT ACTGGCTCAG CCTGGGCGTG CGCGCACGGC GCATCAGCAC CGGCCTCATC 
CTGGTCACTG CGGCCCTGGA GCTCTGCGAG GAGGTGCACC TGTTCGGTTT CTGGGCCTTC 
CCCATGAACC CCTCCGGCTT CTTCATCACG CACCACTACT ATGACAATGT CAAGCCCAAG 
CCCGGCTTTC ATGCTATGCC CTCGGAGATC TTCACCTTCC TGCGCATGCA CAGCCGCGGC 
ATCCTCCGCG TGCACACAGG CACCTGCAAT TGCTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Night/day changes in pineal expression of >600 genes: central role of adrenergic/cAMP signaling.
The Journal of biological chemistry284(12)7606-22(2009 Mar)
Bailey MJ,Coon SL,Carter DA,Humphries A,Kim JS,Shi Q,Gaildrat P,Morin F,Ganguly S,Hogenesch JB,Weller JL,Rath MF,M?ller M,Baler R,Sugden D,Rangel ZG,Munson PJ,Klein DC

The animal sialyltransferases and sialyltransferase-related genes: a phylogenetic approach.
Glycobiology15(8)805-17(2005 Aug)
Harduin-Lepers A,Mollicone R,Delannoy P,Oriol R

Molecular cloning and expression of a fifth type of alpha2,8-sialyltransferase (ST8Sia V). Its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3.
The Journal of biological chemistry271(46)29366-71(1996 Nov)
Kono M,Yoshida Y,Kojima N,Tsuji S