Srgap1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Srgap1
Entrez Gene ID	314903
Full Name	SLIT-ROBO Rho GTPase activating protein 1
Synonyms	RGD1566260
General protein information	Preferred Names SLIT-ROBO Rho GTPase activating protein 1 Names SLIT-ROBO Rho GTPase-activating protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_008765403.2	XP_008763625.2	SLIT-ROBO Rho GTPase-activating protein 1 isoform X1
NM_001191784.1	NP_001178713.1	SLIT-ROBO Rho GTPase-activating protein 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SRGAP1	XP_538265.4
Mus musculus (house mouse)	Srgap1	NP_001074506.1
Danio rerio (zebrafish)	srgap1	XP_005159052.1
Rattus norvegicus (Norway rat)	Srgap1	NP_001178713.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004772	XP_318044.4
Xenopus tropicalis (tropical clawed frog)	srgap1	XP_004912976.1
Pan troglodytes (chimpanzee)	SRGAP1	XP_509190.2
Gallus gallus (chicken)	SRGAP1	NP_001073570.1
Homo sapiens (human)	SRGAP1	NP_065813.1
	SRGAP1	XP_001116844.1
Bos taurus (cattle)	SRGAP1	NP_001192597.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations of the SLIT2-ROBO2 pathway genes SLIT2 and SRGAP1 confer risk for congenital anomalies of the kidney and urinary tract.
Hwang DY, Kohl S, Fan X, Vivante A, Chan S, Dworschak GC, Schulz J, van Eerde AM, Hilger AC, Gee HY, Pennimpede T, Herrmann BG, van de Hoek G, Renkema KY, Schell C, Huber TB, Reutter HM, Soliman NA, Stajic N, Bogdanovic R, Kehinde EO, Lifton RP, Tasic V, Lu W, Hildebrandt F
Human genetics134(8)905-16(2015 Aug)

Regulated shuttling of Slit-Robo-GTPase activating proteins between nucleus and cytoplasm during brain development.
Yao Q, Jin WL, Wang Y, Ju G
Cellular and molecular neurobiology28(2)205-21(2008 Feb)

Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway.
Wong K, Ren XR, Huang YZ, Xie Y, Liu G, Saito H, Tang H, Wen L, Brady-Kalnay SM, Mei L, Wu JY, Xiong WC, Rao Y
Cell107(2)209-21(2001 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations of the SLIT2-ROBO2 pathway genes SLIT2 and SRGAP1 confer risk for congenital anomalies of the kidney and urinary tract
Title: Mutations of the SLIT2-ROBO2 pathway genes SLIT2 and SRGAP1 confer risk for congenital anomalies of the kidney and urinary tract.

results suggest that srGAPs are not only involved in the regulation of the Slit-Robo signal transduction, but also in neuronal development and that the translocation of srGAPs is important for their functions
Title: Regulated shuttling of Slit-Robo-GTPase activating proteins between nucleus and cytoplasm during brain development.

The following Srgap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Srgap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa62015	XM_008765403.2 Latest version!	Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 1 (Srgap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa07649	NM_001191784.1 Latest version!	Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 1 (Srgap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Srgap1 ( XM_008765403.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763625.2 Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 1 (Srgap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa62015
Clone ID Related Accession (Same CDS sequence)	XM_008765403.2
Accession Version	XM_008765403.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	SLIT-ROBO Rho GTPase-activating protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765403.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCACCC CGAGCAGATT CAAGAAGGAC AAAGAGATCA TAGCCGAGTA TGAAAGTCAA 
GTCAAAGAAA TCCGAGCTCA GCTGGTAGAG CAACAAAAAT GCCTGGAGCA GCAGACGGAG 
ATGCGAGTTC AGCTGCTCCA GGACCTACAG GATTTCTTCC GGAAGAAAGC CGAAATTGAG 
ACCGAATATT CTCGGAACCT GGAGAAGCTG GCAGAAAGGT TCATGGCCAA AACAAGAAGC 
ACTAAGGACC ATCAACAGTT CAAGAAAGAC CAGAACCTGT TGTCTCCGGT GAACTGCTGG 
TATTTGCTCC TGAACCAAGT GAGAAGAGAG AGCAAAGACC ACGCCACCTT GAGTGACATC 
TACCTGAACA ACGTCATTAT GCGGTTCATG CAGATAAGCG AGGACTCCAC CCGGATGTTT 
AAAAAGAGCA AAGAGATTGC ATTCCAGCTG CACGAGGACT TAATGAAGGT TCTTAACGAG 
CTTTACACGG TGATGAAAAC CTACCACATG TACCACTCGG AGAGCATCAG TGCAGAAAGC 
AAGCTGAAAG AAGCCGAGAA ACAAGAGGAG AAGCAAATGG GGAGATCTGG TGACCCCGTC 
TTCCACATTA GGCTAGAAGA GAGGCATCAG CGGCGGAGCT CTGTAAAGAA AATTGAAAAA 
ATGAAGGAAA AGAGACAAGC AAAATACTCA GAAAACAAGC TGAAGTCGAT CAAAGCACGC 
AACGAGTATC TCCTAACCCT TGAAGCAACC AACGCCTCTG TGTTCAAGTA TTACATCCAC 
GACCTTTCGG ACTTAATCGA TTGCTGTGAT CTCGGCTACC ATGCAAGTCT GAACAGAGCC 
CTCAGAACAT ACCTCTCGGC GGAGTACAAC CTTGAAACCT CCAGACACGA AGGCTTAGAC 
ATCATTGAAA ATGCTGTGGA CAATTTAGAG CCAAGAAGTG ACAAGCAGAG ATTCATGGAG 
ATGTACCCTG CGGCATTCTG CCCACCAATG AAATTTGAGT TTCAGTCTCA CATGGGCGAT 
GAGGTGTGTC AGGTCAGCGC CCAGCAGCCC GTCCAGGCGG AACTCATGCT CAGGAACCAA 
CAGCTGCAGT CACGACTGGC CACGCTCAAG ATCGAGAGCG AGGAGGTCAA GAAAACAACA 
GAAGCCACCC TCCAGACGAT CCAAGATATG GTCACTATTG AGGACTACGA TGTGTCCGAG 
TGCTTCCAGC ACAGCCGATC CACAGAGTCT GTGAAGTCCA CTGTGTCTGA AACCTATCTG 
AGTAAACCCA GCATCGCCAA GAGACGAGCT AACCAGCAAG AAACTGAGCA GTTCTACTTC 
ATGAAACTTC GAGAGTTCCT GGAAGGCAGT AGCCTCATCA CAAAGCTCCA AGCTAAACAC 
GACTTGCTGC AGAGGACTCT TGGAGAAGGT CATAGAGCTG AATACATGAC TACAAGCCGG 
GGACGAAGGA ACTCTCACGC AAGACACCAG GACTCAGGCC AGGTTATCCC TCTCATTGTG 
GAAAGCTGCA TCCGATTCAT CAATCTCTAT GGTCTTCAGC ATCAGGGGAT TTTCAGAGTG 
TCTGGTTCCC AGGTGGAAGT GAACGATATC AAGAATTCTT TCGAGAGAGG TGAGAATCCT 
CTGGCCGATG ACCAGAGCAA CCATGATATT AACTCTGTGG CCGGTGTTCT GAAACTGTAC 
TTCCGTGGGT TGGAAAACCC CCTCTTTCCT AAGGAAAGGT TTAACGACCT CATTTCGTGT 
ATCAGAATAG ATAACCTCTA TGAGAGGGCA CTCCACATCC ACAAACTCCT CCTGACCTTG 
CCCAGGTCAG TCCTTATAGT GATGAGGTAC CTGTTCGCCT TCCTCAATCA CCTCTCCCAG 
TACAGTGACG AGAACATGAT GGACCCTTAC AATCTGGCCA TTTGCTTTGG CCCAACACTG 
ATGCCAGTTC CGGAAATACA GGACCAAGTG TCTTGCCAGG CACACGTGAA CGAGATCGTG 
AAGACGATCA TCATTCATCA CGAGACTATT TTCCCAGATG CGAAAGAACT GGATGGCCCT 
GTGTACGAGA AATGTATGGC CGGAGGCGAC TACTGCGACA GCCCATACAG TGAGCATGGT 
ACCCTGGAGG AGGTGGACCA AGACGCTGGC ACAGAGCCCC ATACCAGTGA AGACGAGTGT 
GAGCCCATCG AAGCCATAGC CAAGTTTGAC TATGTCGGGC GGTCAGCTAG AGAGCTGTCC 
TTCAAGAAGG GTGCTTCACT GCTGCTGTAT CACCGAGCTT CTGAGGACTG GTGGGAAGGC 
AGACACAACG GCATCGATGG GCTTGTGCCT CATCAGTACA TAGTTGTGCA AGATATGGAC 
GATACATTTT CAGACACGCT GAGCCAAAAA GCCGACAGCG AAGCCAGCAG CGGGCCAGTC 
CCTGAAGACA AGTCCTCATC CAAGGACATG AACTCCCCGA CTGACCGCCA TTCCGATAGC 
TATTTAGCCA GACAAAGAAA GAGAGGAGAA CCACCCCCTC CGGGAAGACG TCCTGGAAGG 
ACCAGTGACG GCCATTGTCC CCTGCATCCC CCACATGCTC TGTCTAACTC CTCGATTGAT 
CTGGGATCCC CAAACCTAAC CGGTCACCCC AGGGGCCTGT TGCAGAACCG TGGCCTCAAC 
AATGACAGTC CTGAGAGGCG ACGCCGGCCG GGCCATGGCA GCCTGACCAA TATCAGCCGC 
CATGACTCCC TCAAGAAGAT AGACAGCCCT CCTATCCGGA GGTCTACGTC ATCAGGGCAG 
TACACCGGCT TCAATGACCA CAAGCCGCTA GACCCCGAGA CAATTGCTCA GGATATTGAA 
GAGACCATGA ATACGGCTTT GAACGAACTC CGTGAGCTGG AGAGGCAGAG CACAGTGAAA 
CACGCCCCGG ATGTCGTGCT AGACACCTTG GAGCAAGTGA AAAATTCGCC CACCCCTGCG 
ACTTCCACAG AATCTCTCAG CCCGCTGCAC AGCGTCACCC TCAGGAGCTC AGAGCCGCAG 
CTTCGACGGA GCACCAGCTC CTCCAGCGAT ACAATGAGTA CTTTCAAGCC TATGGTGGCA 
CCCAGGATGG GTGTCCAGCT GAAGCCCCCA GCCCTTAGGC CAAAGCCTGC TGTTCTTCCA 
AAAACAAACC CTACTGTAGG ACCTGCCGCA CCTTCCCAGG GTCCCACAGA CAAGTCTTGC 
ACGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763625.2
CDS	340..3528
Translation	MSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQTEMRVQLLQDLQDFFRKKAEIETEYSRNLEKLAERFMAKTRSTKDHQQFKKDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTRMFKKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHSESISAESKLKEAEKQEEKQMGRSGDPVFHIRLEERHQRRSSVKKIEKMKEKRQAKYSENKLKSIKARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAEYNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHMGDEVCQVSAQQPVQAELMLRNQQLQSRLATLKIESEEVKKTTEATLQTIQDMVTIEDYDVSECFQHSRSTESVKSTVSETYLSKPSIAKRRANQQETEQFYFMKLREFLEGSSLITKLQAKHDLLQRTLGEGHRAEYMTTSRGRRNSHARHQDSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERGENPLADDQSNHDINSVAGVLKLYFRGLENPLFPKERFNDLISCIRIDNLYERALHIHKLLLTLPRSVLIVMRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMPVPEIQDQVSCQAHVNEIVKTIIIHHETIFPDAKELDGPVYEKCMAGGDYCDSPYSEHGTLEEVDQDAGTEPHTSEDECEPIEAIAKFDYVGRSARELSFKKGASLLLYHRASEDWWEGRHNGIDGLVPHQYIVVQDMDDTFSDTLSQKADSEASSGPVPEDKSSSKDMNSPTDRHSDSYLARQRKRGEPPPPGRRPGRTSDGHCPLHPPHALSNSSIDLGSPNLTGHPRGLLQNRGLNNDSPERRRRPGHGSLTNISRHDSLKKIDSPPIRRSTSSGQYTGFNDHKPLDPETIAQDIEETMNTALNELRELERQSTVKHAPDVVLDTLEQVKNSPTPATSTESLSPLHSVTLRSSEPQLRRSTSSSSDTMSTFKPMVAPRMGVQLKPPALRPKPAVLPKTNPTVGPAAPSQGPTDKSCTM

Target ORF information:

RefSeq Version	XM_008765403.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 1 (Srgap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765403.2

ORF Insert Sequence:

ATGTCCACCC CGAGCAGATT CAAGAAGGAC AAAGAGATCA TAGCCGAGTA TGAAAGTCAA 
GTCAAAGAAA TCCGAGCTCA GCTGGTAGAG CAACAAAAAT GCCTGGAGCA GCAGACGGAG 
ATGCGAGTTC AGCTGCTCCA GGACCTACAG GATTTCTTCC GGAAGAAAGC CGAAATTGAG 
ACCGAATATT CTCGGAACCT GGAGAAGCTG GCAGAAAGGT TCATGGCCAA AACAAGAAGC 
ACTAAGGACC ATCAACAGTT CAAGAAAGAC CAGAACCTGT TGTCTCCGGT GAACTGCTGG 
TATTTGCTCC TGAACCAAGT GAGAAGAGAG AGCAAAGACC ACGCCACCTT GAGTGACATC 
TACCTGAACA ACGTCATTAT GCGGTTCATG CAGATAAGCG AGGACTCCAC CCGGATGTTT 
AAAAAGAGCA AAGAGATTGC ATTCCAGCTG CACGAGGACT TAATGAAGGT TCTTAACGAG 
CTTTACACGG TGATGAAAAC CTACCACATG TACCACTCGG AGAGCATCAG TGCAGAAAGC 
AAGCTGAAAG AAGCCGAGAA ACAAGAGGAG AAGCAAATGG GGAGATCTGG TGACCCCGTC 
TTCCACATTA GGCTAGAAGA GAGGCATCAG CGGCGGAGCT CTGTAAAGAA AATTGAAAAA 
ATGAAGGAAA AGAGACAAGC AAAATACTCA GAAAACAAGC TGAAGTCGAT CAAAGCACGC 
AACGAGTATC TCCTAACCCT TGAAGCAACC AACGCCTCTG TGTTCAAGTA TTACATCCAC 
GACCTTTCGG ACTTAATCGA TTGCTGTGAT CTCGGCTACC ATGCAAGTCT GAACAGAGCC 
CTCAGAACAT ACCTCTCGGC GGAGTACAAC CTTGAAACCT CCAGACACGA AGGCTTAGAC 
ATCATTGAAA ATGCTGTGGA CAATTTAGAG CCAAGAAGTG ACAAGCAGAG ATTCATGGAG 
ATGTACCCTG CGGCATTCTG CCCACCAATG AAATTTGAGT TTCAGTCTCA CATGGGCGAT 
GAGGTGTGTC AGGTCAGCGC CCAGCAGCCC GTCCAGGCGG AACTCATGCT CAGGAACCAA 
CAGCTGCAGT CACGACTGGC CACGCTCAAG ATCGAGAGCG AGGAGGTCAA GAAAACAACA 
GAAGCCACCC TCCAGACGAT CCAAGATATG GTCACTATTG AGGACTACGA TGTGTCCGAG 
TGCTTCCAGC ACAGCCGATC CACAGAGTCT GTGAAGTCCA CTGTGTCTGA AACCTATCTG 
AGTAAACCCA GCATCGCCAA GAGACGAGCT AACCAGCAAG AAACTGAGCA GTTCTACTTC 
ATGAAACTTC GAGAGTTCCT GGAAGGCAGT AGCCTCATCA CAAAGCTCCA AGCTAAACAC 
GACTTGCTGC AGAGGACTCT TGGAGAAGGT CATAGAGCTG AATACATGAC TACAAGCCGG 
GGACGAAGGA ACTCTCACGC AAGACACCAG GACTCAGGCC AGGTTATCCC TCTCATTGTG 
GAAAGCTGCA TCCGATTCAT CAATCTCTAT GGTCTTCAGC ATCAGGGGAT TTTCAGAGTG 
TCTGGTTCCC AGGTGGAAGT GAACGATATC AAGAATTCTT TCGAGAGAGG TGAGAATCCT 
CTGGCCGATG ACCAGAGCAA CCATGATATT AACTCTGTGG CCGGTGTTCT GAAACTGTAC 
TTCCGTGGGT TGGAAAACCC CCTCTTTCCT AAGGAAAGGT TTAACGACCT CATTTCGTGT 
ATCAGAATAG ATAACCTCTA TGAGAGGGCA CTCCACATCC ACAAACTCCT CCTGACCTTG 
CCCAGGTCAG TCCTTATAGT GATGAGGTAC CTGTTCGCCT TCCTCAATCA CCTCTCCCAG 
TACAGTGACG AGAACATGAT GGACCCTTAC AATCTGGCCA TTTGCTTTGG CCCAACACTG 
ATGCCAGTTC CGGAAATACA GGACCAAGTG TCTTGCCAGG CACACGTGAA CGAGATCGTG 
AAGACGATCA TCATTCATCA CGAGACTATT TTCCCAGATG CGAAAGAACT GGATGGCCCT 
GTGTACGAGA AATGTATGGC CGGAGGCGAC TACTGCGACA GCCCATACAG TGAGCATGGT 
ACCCTGGAGG AGGTGGACCA AGACGCTGGC ACAGAGCCCC ATACCAGTGA AGACGAGTGT 
GAGCCCATCG AAGCCATAGC CAAGTTTGAC TATGTCGGGC GGTCAGCTAG AGAGCTGTCC 
TTCAAGAAGG GTGCTTCACT GCTGCTGTAT CACCGAGCTT CTGAGGACTG GTGGGAAGGC 
AGACACAACG GCATCGATGG GCTTGTGCCT CATCAGTACA TAGTTGTGCA AGATATGGAC 
GATACATTTT CAGACACGCT GAGCCAAAAA GCCGACAGCG AAGCCAGCAG CGGGCCAGTC 
CCTGAAGACA AGTCCTCATC CAAGGACATG AACTCCCCGA CTGACCGCCA TTCCGATAGC 
TATTTAGCCA GACAAAGAAA GAGAGGAGAA CCACCCCCTC CGGGAAGACG TCCTGGAAGG 
ACCAGTGACG GCCATTGTCC CCTGCATCCC CCACATGCTC TGTCTAACTC CTCGATTGAT 
CTGGGATCCC CAAACCTAAC CGGTCACCCC AGGGGCCTGT TGCAGAACCG TGGCCTCAAC 
AATGACAGTC CTGAGAGGCG ACGCCGGCCG GGCCATGGCA GCCTGACCAA TATCAGCCGC 
CATGACTCCC TCAAGAAGAT AGACAGCCCT CCTATCCGGA GGTCTACGTC ATCAGGGCAG 
TACACCGGCT TCAATGACCA CAAGCCGCTA GACCCCGAGA CAATTGCTCA GGATATTGAA 
GAGACCATGA ATACGGCTTT GAACGAACTC CGTGAGCTGG AGAGGCAGAG CACAGTGAAA 
CACGCCCCGG ATGTCGTGCT AGACACCTTG GAGCAAGTGA AAAATTCGCC CACCCCTGCG 
ACTTCCACAG AATCTCTCAG CCCGCTGCAC AGCGTCACCC TCAGGAGCTC AGAGCCGCAG 
CTTCGACGGA GCACCAGCTC CTCCAGCGAT ACAATGAGTA CTTTCAAGCC TATGGTGGCA 
CCCAGGATGG GTGTCCAGCT GAAGCCCCCA GCCCTTAGGC CAAAGCCTGC TGTTCTTCCA 
AAAACAAACC CTACTGTAGG ACCTGCCGCA CCTTCCCAGG GTCCCACAGA CAAGTCTTGC 
ACGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Srgap1 ( NM_001191784.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001178713.1 Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 1 (Srgap1), mRNA.

Price & Availability↑

CloneID	ORa07649
Clone ID Related Accession (Same CDS sequence)	NM_001191784.1
Accession Version	NM_001191784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-31
Organism	Rattus norvegicus(Norway rat)
Product	SLIT-ROBO Rho GTPase-activating protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07057276.1 and AABR07057274.1. On Jul 17, 2010 this sequence version replaced XM_235213.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689601 [ECO:0000348] ##Evidence-Data-END##

ATGTCCACCC CGAGCAGATT CAAGAAGGAC AAAGAGATCA TAGCCGAGTA TGAAAGTCAA 
GTCAAAGAAA TCCGAGCTCA GCTGGTAGAG CAACAAAAAT GCCTGGAGCA GCAGACGGAG 
ATGCGAGTTC AGCTGCTCCA GGACCTACAG GATTTCTTCC GGAAGAAAGC CGAAATTGAG 
ACCGAATATT CTCGGAACCT GGAGAAGCTG GCAGAAAGGT TCATGGCCAA AACAAGAAGC 
ACTAAGGACC ATCAACAGTT CAAGAAAGAC CAGAACCTGT TGTCTCCGGT GAACTGCTGG 
TATTTGCTCC TGAACCAAGT GAGAAGAGAG AGCAAAGACC ACGCCACCTT GAGTGACATC 
TACCTGAACA ACGTCATTAT GCGGTTCATG CAGATAAGCG AGGACTCCAC CCGGATGTTT 
AAAAAGAGCA AAGAGATTGC ATTCCAGCTG CACGAGGACT TAATGAAGGT TCTTAACGAG 
CTTTACACGG TGATGAAAAC CTACCACATG TACCACTCGG AGAGCATCAG TGCAGAAAGC 
AAGCTGAAAG AAGCCGAGAA ACAAGAGGAG AAGCAAATGG GGAGATCTGG TGACCCCGTC 
TTCCACATTA GGCTAGAAGA GAGGCATCAG CGGCGGAGCT CTGTAAAGAA AATTGAAAAA 
ATGAAGGAAA AGAGACAAGC AAAATACTCA GAAAACAAGC TGAAGTCGAT CAAAGCACGC 
AACGAGTATC TCCTAACCCT TGAAGCAACC AACGCCTCTG TGTTCAAGTA TTACATCCAC 
GACCTTTCGG ACTTAATCGA TTGCTGTGAT CTCGGCTACC ATGCAAGTCT GAACAGAGCC 
CTCAGAACAT ACCTCTCGGC GGAGTACAAC CTTGAAACCT CCAGACACGA AGGCTTAGAC 
ATCATTGAAA ATGCTGTGGA CAATTTAGAG CCAAGAAGTG ACAAGCAGAG ATTCATGGAG 
ATGTACCCTG CGGCATTCTG CCCACCAATG AAATTTGAGT TTCAGTCTCA CATGGGCGAT 
GAGGTGTGTC AGGTCAGCGC CCAGCAGCCC GTCCAGGCGG AACTCATGCT CAGGAACCAA 
CAGCTGCAGT CACGACTGGC CACGCTCAAG ATCGAGAGCG AGGAGGTCAA GAAAACAACA 
GAAGCCACCC TCCAGACGAT CCAAGATATG GTCACTATTG AGGACTACGA TGTGTCCGAG 
TGCTTCCAGC ACAGCCGATC CACAGAGTCT GTGAAGTCCA CTGTGTCTGA AACCTATCTG 
AGTAAACCCA GCATCGCCAA GAGACGAGCT AACCAGCAAG AAACTGAGCA GTTCTACTTC 
ATGAAACTTC GAGAGTTCCT GGAAGGCAGT AGCCTCATCA CAAAGCTCCA AGCTAAACAC 
GACTTGCTGC AGAGGACTCT TGGAGAAGGT CATAGAGCTG AATACATGAC TACAAGGCCT 
CCAAACGTCC CCCCTAAGCC CCAGAAACAC AGGAAGTCCA GGCCCCGCTC CCAGTATAAT 
GCTAAGTTGT TTAATGGGGA TTTGGAGACA TTCGTCAAGG ACTCAGGCCA GGTTATCCCT 
CTCATTGTGG AAAGCTGCAT CCGATTCATC AATCTCTATG GTCTTCAGCA TCAGGGGATT 
TTCAGAGTGT CTGGTTCCCA GGTGGAAGTG AACGATATCA AGAATTCTTT CGAGAGAGGT 
GAGAATCCTC TGGCCGATGA CCAGAGCAAC CATGATATTA ACTCTGTGGC CGGTGTTCTG 
AAACTGTACT TCCGTGGGTT GGAAAACCCC CTCTTTCCTA AGGAAAGGTT TAACGACCTC 
ATTTCGTGTA TCAGAATAGA TAACCTCTAT GAGAGGGCAC TCCACATCCA CAAACTCCTC 
CTGACCTTGC CCAGGTCAGT CCTTATAGTG ATGAGGTACC TGTTCGCCTT CCTCAATCAC 
CTCTCCCAGT ACAGTGACGA GAACATGATG GACCCTTACA ATCTGGCCAT TTGCTTTGGC 
CCAACACTGA TGCCAGTTCC GGAAATACAG GACCAAGTGT CTTGCCAGGC ACACGTGAAC 
GAGATCGTGA AGACGATCAT CATTCATCAC GAGACTATTT TCCCAGATGC GAAAGAACTG 
GATGGCCCTG TGTACGAGAA ATGTATGGCC GGAGGCGACT ACTGCGACAG CCCATACAGT 
GAGCATGGTA CCCTGGAGGA GGTGGACCAA GACGCTGGCA CAGAGCCCCA TACCAGTGAA 
GACGAGTGTG AGCCCATCGA AGCCATAGCC AAGTTTGACT ATGTCGGGCG GTCAGCTAGA 
GAGCTGTCCT TCAAGAAGGG TGCTTCACTG CTGCTGTATC ACCGAGCTTC TGAGGACTGG 
TGGGAAGGCA GACACAACGG CATCGATGGG CTTGTGCCTC ATCAGTACAT AGTTGTGCAA 
GATATGGACG ATACATTTTC AGACACGCTG AGCCAAAAAG CCGACAGCGA AGCCAGCAGC 
GGGCCAGTCC CTGAAGACAA GTCCTCATCC AAGGACATGA ACTCCCCGAC TGACCGCCAT 
TCCGATAGCT ATTTAGCCAG ACAAAGAAAG AGAGGAGAAC CACCCCCTCC GGGAAGACGT 
CCTGGAAGGA CCAGTGACGG CCATTGTCCC CTGCATCCCC CACATGCTCT GTCTAACTCC 
TCGATTGATC TGGGATCCCC AAACCTAACC GGTCACCCCA GGGGCCTGTT GCAGAACCGT 
GGCCTCAACA ATGACAGTCC TGAGAGGCGA CGCCGGCCGG GCCATGGCAG CCTGACCAAT 
ATCAGCCGCC ATGACTCCCT CAAGAAGATA GACAGCCCTC CTATCCGGAG GTCTACGTCA 
TCAGGGCAGT ACACCGGCTT CAATGACCAC AAGCCGCTAG ACCCCGAGAC AATTGCTCAG 
GATATTGAAG AGACCATGAA TACGGCTTTG AACGAACTCC GTGAGCTGGA GAGGCAGAGC 
ACAGTGAAAC ACGCCCCGGA TGTCGTGCTA GACACCTTGG AGCAAGTGAA AAATTCGCCC 
ACCCCTGCGA CTTCCACAGA ATCTCTCAGC CCGCTGCACA GCGTCACCCT CAGGAGCTCA 
GAGCCGCAGC TTCGACGGAG CACCAGCTCC TCCAGCGATA CAATGAGTAC TTTCAAGCCT 
ATGGTGGCAC CCAGGATGGG TGTCCAGCTG AAGCCCCCAG CCCTTAGGCC AAAGCCTGCT 
GTTCTTCCAA AAACAAACCC TACTGTAGGA CCTGCCGCAC CTTCCCAGGG TCCCACAGAC 
AAGTCTTGCA CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178713.1
CDS	1..3258
Translation	MSTPSRFKKDKEIIAEYESQVKEIRAQLVEQQKCLEQQTEMRVQLLQDLQDFFRKKAEIETEYSRNLEKLAERFMAKTRSTKDHQQFKKDQNLLSPVNCWYLLLNQVRRESKDHATLSDIYLNNVIMRFMQISEDSTRMFKKSKEIAFQLHEDLMKVLNELYTVMKTYHMYHSESISAESKLKEAEKQEEKQMGRSGDPVFHIRLEERHQRRSSVKKIEKMKEKRQAKYSENKLKSIKARNEYLLTLEATNASVFKYYIHDLSDLIDCCDLGYHASLNRALRTYLSAEYNLETSRHEGLDIIENAVDNLEPRSDKQRFMEMYPAAFCPPMKFEFQSHMGDEVCQVSAQQPVQAELMLRNQQLQSRLATLKIESEEVKKTTEATLQTIQDMVTIEDYDVSECFQHSRSTESVKSTVSETYLSKPSIAKRRANQQETEQFYFMKLREFLEGSSLITKLQAKHDLLQRTLGEGHRAEYMTTRPPNVPPKPQKHRKSRPRSQYNAKLFNGDLETFVKDSGQVIPLIVESCIRFINLYGLQHQGIFRVSGSQVEVNDIKNSFERGENPLADDQSNHDINSVAGVLKLYFRGLENPLFPKERFNDLISCIRIDNLYERALHIHKLLLTLPRSVLIVMRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMPVPEIQDQVSCQAHVNEIVKTIIIHHETIFPDAKELDGPVYEKCMAGGDYCDSPYSEHGTLEEVDQDAGTEPHTSEDECEPIEAIAKFDYVGRSARELSFKKGASLLLYHRASEDWWEGRHNGIDGLVPHQYIVVQDMDDTFSDTLSQKADSEASSGPVPEDKSSSKDMNSPTDRHSDSYLARQRKRGEPPPPGRRPGRTSDGHCPLHPPHALSNSSIDLGSPNLTGHPRGLLQNRGLNNDSPERRRRPGHGSLTNISRHDSLKKIDSPPIRRSTSSGQYTGFNDHKPLDPETIAQDIEETMNTALNELRELERQSTVKHAPDVVLDTLEQVKNSPTPATSTESLSPLHSVTLRSSEPQLRRSTSSSSDTMSTFKPMVAPRMGVQLKPPALRPKPAVLPKTNPTVGPAAPSQGPTDKSCTM

Target ORF information:

RefSeq Version	NM_001191784.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus SLIT-ROBO Rho GTPase activating protein 1 (Srgap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191784.1

ORF Insert Sequence:

ATGTCCACCC CGAGCAGATT CAAGAAGGAC AAAGAGATCA TAGCCGAGTA TGAAAGTCAA 
GTCAAAGAAA TCCGAGCTCA GCTGGTAGAG CAACAAAAAT GCCTGGAGCA GCAGACGGAG 
ATGCGAGTTC AGCTGCTCCA GGACCTACAG GATTTCTTCC GGAAGAAAGC CGAAATTGAG 
ACCGAATATT CTCGGAACCT GGAGAAGCTG GCAGAAAGGT TCATGGCCAA AACAAGAAGC 
ACTAAGGACC ATCAACAGTT CAAGAAAGAC CAGAACCTGT TGTCTCCGGT GAACTGCTGG 
TATTTGCTCC TGAACCAAGT GAGAAGAGAG AGCAAAGACC ACGCCACCTT GAGTGACATC 
TACCTGAACA ACGTCATTAT GCGGTTCATG CAGATAAGCG AGGACTCCAC CCGGATGTTT 
AAAAAGAGCA AAGAGATTGC ATTCCAGCTG CACGAGGACT TAATGAAGGT TCTTAACGAG 
CTTTACACGG TGATGAAAAC CTACCACATG TACCACTCGG AGAGCATCAG TGCAGAAAGC 
AAGCTGAAAG AAGCCGAGAA ACAAGAGGAG AAGCAAATGG GGAGATCTGG TGACCCCGTC 
TTCCACATTA GGCTAGAAGA GAGGCATCAG CGGCGGAGCT CTGTAAAGAA AATTGAAAAA 
ATGAAGGAAA AGAGACAAGC AAAATACTCA GAAAACAAGC TGAAGTCGAT CAAAGCACGC 
AACGAGTATC TCCTAACCCT TGAAGCAACC AACGCCTCTG TGTTCAAGTA TTACATCCAC 
GACCTTTCGG ACTTAATCGA TTGCTGTGAT CTCGGCTACC ATGCAAGTCT GAACAGAGCC 
CTCAGAACAT ACCTCTCGGC GGAGTACAAC CTTGAAACCT CCAGACACGA AGGCTTAGAC 
ATCATTGAAA ATGCTGTGGA CAATTTAGAG CCAAGAAGTG ACAAGCAGAG ATTCATGGAG 
ATGTACCCTG CGGCATTCTG CCCACCAATG AAATTTGAGT TTCAGTCTCA CATGGGCGAT 
GAGGTGTGTC AGGTCAGCGC CCAGCAGCCC GTCCAGGCGG AACTCATGCT CAGGAACCAA 
CAGCTGCAGT CACGACTGGC CACGCTCAAG ATCGAGAGCG AGGAGGTCAA GAAAACAACA 
GAAGCCACCC TCCAGACGAT CCAAGATATG GTCACTATTG AGGACTACGA TGTGTCCGAG 
TGCTTCCAGC ACAGCCGATC CACAGAGTCT GTGAAGTCCA CTGTGTCTGA AACCTATCTG 
AGTAAACCCA GCATCGCCAA GAGACGAGCT AACCAGCAAG AAACTGAGCA GTTCTACTTC 
ATGAAACTTC GAGAGTTCCT GGAAGGCAGT AGCCTCATCA CAAAGCTCCA AGCTAAACAC 
GACTTGCTGC AGAGGACTCT TGGAGAAGGT CATAGAGCTG AATACATGAC TACAAGGCCT 
CCAAACGTCC CCCCTAAGCC CCAGAAACAC AGGAAGTCCA GGCCCCGCTC CCAGTATAAT 
GCTAAGTTGT TTAATGGGGA TTTGGAGACA TTCGTCAAGG ACTCAGGCCA GGTTATCCCT 
CTCATTGTGG AAAGCTGCAT CCGATTCATC AATCTCTATG GTCTTCAGCA TCAGGGGATT 
TTCAGAGTGT CTGGTTCCCA GGTGGAAGTG AACGATATCA AGAATTCTTT CGAGAGAGGT 
GAGAATCCTC TGGCCGATGA CCAGAGCAAC CATGATATTA ACTCTGTGGC CGGTGTTCTG 
AAACTGTACT TCCGTGGGTT GGAAAACCCC CTCTTTCCTA AGGAAAGGTT TAACGACCTC 
ATTTCGTGTA TCAGAATAGA TAACCTCTAT GAGAGGGCAC TCCACATCCA CAAACTCCTC 
CTGACCTTGC CCAGGTCAGT CCTTATAGTG ATGAGGTACC TGTTCGCCTT CCTCAATCAC 
CTCTCCCAGT ACAGTGACGA GAACATGATG GACCCTTACA ATCTGGCCAT TTGCTTTGGC 
CCAACACTGA TGCCAGTTCC GGAAATACAG GACCAAGTGT CTTGCCAGGC ACACGTGAAC 
GAGATCGTGA AGACGATCAT CATTCATCAC GAGACTATTT TCCCAGATGC GAAAGAACTG 
GATGGCCCTG TGTACGAGAA ATGTATGGCC GGAGGCGACT ACTGCGACAG CCCATACAGT 
GAGCATGGTA CCCTGGAGGA GGTGGACCAA GACGCTGGCA CAGAGCCCCA TACCAGTGAA 
GACGAGTGTG AGCCCATCGA AGCCATAGCC AAGTTTGACT ATGTCGGGCG GTCAGCTAGA 
GAGCTGTCCT TCAAGAAGGG TGCTTCACTG CTGCTGTATC ACCGAGCTTC TGAGGACTGG 
TGGGAAGGCA GACACAACGG CATCGATGGG CTTGTGCCTC ATCAGTACAT AGTTGTGCAA 
GATATGGACG ATACATTTTC AGACACGCTG AGCCAAAAAG CCGACAGCGA AGCCAGCAGC 
GGGCCAGTCC CTGAAGACAA GTCCTCATCC AAGGACATGA ACTCCCCGAC TGACCGCCAT 
TCCGATAGCT ATTTAGCCAG ACAAAGAAAG AGAGGAGAAC CACCCCCTCC GGGAAGACGT 
CCTGGAAGGA CCAGTGACGG CCATTGTCCC CTGCATCCCC CACATGCTCT GTCTAACTCC 
TCGATTGATC TGGGATCCCC AAACCTAACC GGTCACCCCA GGGGCCTGTT GCAGAACCGT 
GGCCTCAACA ATGACAGTCC TGAGAGGCGA CGCCGGCCGG GCCATGGCAG CCTGACCAAT 
ATCAGCCGCC ATGACTCCCT CAAGAAGATA GACAGCCCTC CTATCCGGAG GTCTACGTCA 
TCAGGGCAGT ACACCGGCTT CAATGACCAC AAGCCGCTAG ACCCCGAGAC AATTGCTCAG 
GATATTGAAG AGACCATGAA TACGGCTTTG AACGAACTCC GTGAGCTGGA GAGGCAGAGC 
ACAGTGAAAC ACGCCCCGGA TGTCGTGCTA GACACCTTGG AGCAAGTGAA AAATTCGCCC 
ACCCCTGCGA CTTCCACAGA ATCTCTCAGC CCGCTGCACA GCGTCACCCT CAGGAGCTCA 
GAGCCGCAGC TTCGACGGAG CACCAGCTCC TCCAGCGATA CAATGAGTAC TTTCAAGCCT 
ATGGTGGCAC CCAGGATGGG TGTCCAGCTG AAGCCCCCAG CCCTTAGGCC AAAGCCTGCT 
GTTCTTCCAA AAACAAACCC TACTGTAGGA CCTGCCGCAC CTTCCCAGGG TCCCACAGAC 
AAGTCTTGCA CGATGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations of the SLIT2-ROBO2 pathway genes SLIT2 and SRGAP1 confer risk for congenital anomalies of the kidney and urinary tract.
Human genetics134(8)905-16(2015 Aug)
Hwang DY,Kohl S,Fan X,Vivante A,Chan S,Dworschak GC,Schulz J,van Eerde AM,Hilger AC,Gee HY,Pennimpede T,Herrmann BG,van de Hoek G,Renkema KY,Schell C,Huber TB,Reutter HM,Soliman NA,Stajic N,Bogdanovic R,Kehinde EO,Lifton RP,Tasic V,Lu W,Hildebrandt F

Regulated shuttling of Slit-Robo-GTPase activating proteins between nucleus and cytoplasm during brain development.
Cellular and molecular neurobiology28(2)205-21(2008 Feb)
Yao Q,Jin WL,Wang Y,Ju G

Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway.
Cell107(2)209-21(2001 Oct)
Wong K,Ren XR,Huang YZ,Xie Y,Liu G,Saito H,Tang H,Wen L,Brady-Kalnay SM,Mei L,Wu JY,Xiong WC,Rao Y