Pcgf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pcgf1
Entrez Gene ID	312480
Full Name	polycomb group ring finger 1
Synonyms	Nspc1
General protein information	Preferred Names polycomb group ring finger 1 Names polycomb group RING finger protein 1 nervous system Polycomb-1 nervous system polycomb 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q34

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007000.1	NP_001007001.1	polycomb group RING finger protein 1

Pathways from BioSystems

Homologies

	LOC715341	XP_001105519.2
Pan troglodytes (chimpanzee)	PCGF1	XP_515562.2
Canis lupus familiaris (dog)	PCGF1	XP_532995.2
Bos taurus (cattle)	PCGF1	NP_001039912.2
Homo sapiens (human)	PCGF1	NP_116062.2
Rattus norvegicus (Norway rat)	Pcgf1	NP_001007001.1
Danio rerio (zebrafish)	pcgf1	NP_001007159.1
Mus musculus (house mouse)	Pcgf1	NP_932109.1
Gallus gallus (chicken)	PCGF1	XP_003643507.2

Gene Ontology
Bibliography

Related articles in PubMed

PCGF3/5-PRC1 initiates Polycomb recruitment in X chromosome inactivation.
Almeida M, Pintacuda G, Masui O, Koseki Y, Gdula M, Cerase A, Brown D, Mould A, Innocent C, Nakayama M, Schermelleh L, Nesterova TB, Koseki H, Brockdorff N
Science (New York, N.Y.)356(6342)1081-1084(2017 06)

BMI1-RING1B is an autoinhibited RING E3 ubiquitin ligase.
Taherbhoy AM, Huang OW, Cochran AG
Nature communications67621(2015 Jul)

PRC1 complex diversity: where is it taking us?
Gil J, O'Loghlen A
Trends in cell biology24(11)632-41(2014 Nov)

Occupying chromatin: Polycomb mechanisms for getting to genomic targets, stopping transcriptional traffic, and staying put.
Simon JA, Kingston RE
Molecular cell49(5)808-24(2013 Mar)

Age-related alterations in retinal neurovascular and inflammatory transcripts.
Van Kirk CA, VanGuilder HD, Young M, Farley JA, Sonntag WE, Freeman WM
Molecular vision171261-74(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pcgf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pcgf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10975	NM_001007000.1 Latest version!	Rattus norvegicus polycomb group ring finger 1 (Pcgf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pcgf1 ( NM_001007000.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001007001.1 Rattus norvegicus polycomb group ring finger 1 (Pcgf1), mRNA.

Price & Availability↑

CloneID	ORa10975
Clone ID Related Accession (Same CDS sequence)	NM_001007000.1
Accession Version	NM_001007000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	polycomb group RING finger protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY662670.1. On Nov 22, 2004 this sequence version replaced XM_232109.2. ##Evidence-Data-START## Transcript exon combination :: AY662670.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGGCTTC GGAACCAGCT CCAGTCAGTG TACAAGATGG ACCCACTACG GAACGAGGAG 
GAGGTCCGGG TGAAGATCAA AGACCTGAAT GAACACATCG TTTGCTGCCT ATGCGCGGGC 
TACTTCGTGG ACGCCACCAC TATCACAGAG TGCCTCCACA CCTTCTGCAA AAGTTGTATC 
GTGAAGTACC TGCAGACCAG CAAGTACTGC CCCATGTGTA ACATCAAGAT CCACGAGACG 
CAGCCGTTGC TCAATCTCAA ACTGGATCGG GTCATGCAGG ACATAGTGTA TAAGCTAGTA 
CCAGGCTTGC AAGACAGTGA AGAGAAACGG ATTCGGGATT TCTATCAGTC CCGAGGCTTG 
GACAGAGTCT CCCAGCCCAG TGGTGAAGAG CCAGCCCTGA GGGGCCTGGG CCTCCCCTTC 
ACCAGTTTTG ACCACTATTA TCGATACGAT GAGCAGCTGA GCCTATGCCT GGAGCGGCTG 
AGTTCTGGCA AAGACAAGAA TAAAAATGTC CTTCAGAACA AGTATGTTCG ATGTTCTGTT 
AGAGCTGAGG TCCGCCATCT CCGAAGGGTT CTGTGTCACC GACTAATGCT AAATCCACAA 
CATGTACAGC TCCTTTTTGA CAATGAGGTT CTCCCTGATC ACATGACAAT GAAGCAGCTA 
TGGCTTTCCC GCTGGTTCGG CAAGCCATCT CCTTTGCTTC TACAATACAG TGTGAAAGAG 
AAGAGGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007001.1
CDS	1..732
Translation	MRLRNQLQSVYKMDPLRNEEEVRVKIKDLNEHIVCCLCAGYFVDATTITECLHTFCKSCIVKYLQTSKYCPMCNIKIHETQPLLNLKLDRVMQDIVYKLVPGLQDSEEKRIRDFYQSRGLDRVSQPSGEEPALRGLGLPFTSFDHYYRYDEQLSLCLERLSSGKDKNKNVLQNKYVRCSVRAEVRHLRRVLCHRLMLNPQHVQLLFDNEVLPDHMTMKQLWLSRWFGKPSPLLLQYSVKEKRR

Target ORF information:

RefSeq Version	NM_001007000.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polycomb group ring finger 1 (Pcgf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007000.1

ORF Insert Sequence:

ATGAGGCTTC GGAACCAGCT CCAGTCAGTG TACAAGATGG ACCCACTACG GAACGAGGAG 
GAGGTCCGGG TGAAGATCAA AGACCTGAAT GAACACATCG TTTGCTGCCT ATGCGCGGGC 
TACTTCGTGG ACGCCACCAC TATCACAGAG TGCCTCCACA CCTTCTGCAA AAGTTGTATC 
GTGAAGTACC TGCAGACCAG CAAGTACTGC CCCATGTGTA ACATCAAGAT CCACGAGACG 
CAGCCGTTGC TCAATCTCAA ACTGGATCGG GTCATGCAGG ACATAGTGTA TAAGCTAGTA 
CCAGGCTTGC AAGACAGTGA AGAGAAACGG ATTCGGGATT TCTATCAGTC CCGAGGCTTG 
GACAGAGTCT CCCAGCCCAG TGGTGAAGAG CCAGCCCTGA GGGGCCTGGG CCTCCCCTTC 
ACCAGTTTTG ACCACTATTA TCGATACGAT GAGCAGCTGA GCCTATGCCT GGAGCGGCTG 
AGTTCTGGCA AAGACAAGAA TAAAAATGTC CTTCAGAACA AGTATGTTCG ATGTTCTGTT 
AGAGCTGAGG TCCGCCATCT CCGAAGGGTT CTGTGTCACC GACTAATGCT AAATCCACAA 
CATGTACAGC TCCTTTTTGA CAATGAGGTT CTCCCTGATC ACATGACAAT GAAGCAGCTA 
TGGCTTTCCC GCTGGTTCGG CAAGCCATCT CCTTTGCTTC TACAATACAG TGTGAAAGAG 
AAGAGGAGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PCGF3/5-PRC1 initiates Polycomb recruitment in X chromosome inactivation.
Science (New York, N.Y.)356(6342)1081-1084(2017 06)
Almeida M,Pintacuda G,Masui O,Koseki Y,Gdula M,Cerase A,Brown D,Mould A,Innocent C,Nakayama M,Schermelleh L,Nesterova TB,Koseki H,Brockdorff N

BMI1-RING1B is an autoinhibited RING E3 ubiquitin ligase.
Nature communications67621(2015 Jul)
Taherbhoy AM,Huang OW,Cochran AG

PRC1 complex diversity: where is it taking us?
Trends in cell biology24(11)632-41(2014 Nov)
Gil J,O'Loghlen A

Occupying chromatin: Polycomb mechanisms for getting to genomic targets, stopping transcriptional traffic, and staying put.
Molecular cell49(5)808-24(2013 Mar)
Simon JA,Kingston RE

Age-related alterations in retinal neurovascular and inflammatory transcripts.
Molecular vision171261-74(2011)
Van Kirk CA,VanGuilder HD,Young M,Farley JA,Sonntag WE,Freeman WM