Spint1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spint1
Entrez Gene ID	311331
Full Name	serine peptidase inhibitor, Kunitz type 1
Synonyms	HAI-1
General protein information	Preferred Names serine peptidase inhibitor, Kunitz type 1 Names kunitz-type protease inhibitor 1 hepatocyte growth factor activator inhibitor-1 serine protease inhibitor, Kunitz type 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q35
Summary	human homolog inhibits the serine proteases hepatocyte growth factor activator (HGFA) and matriptase, which regulate hepatocyte growth factor processing and maturation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006234763.2	XP_006234825.1	kunitz-type protease inhibitor 1 isoform X1
NM_001004265.2	NP_001004265.2	kunitz-type protease inhibitor 1 precursor
XM_006234764.2	XP_006234826.1	kunitz-type protease inhibitor 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPINT1	NP_857593.1
Pan troglodytes (chimpanzee)	SPINT1	XP_003314674.1
	SPINT1	XP_001098832.1
Canis lupus familiaris (dog)	SPINT1	XP_544625.4
Gallus gallus (chicken)	SPINT1	XP_004941670.1
Xenopus tropicalis (tropical clawed frog)	spint1	XP_002939591.1
Mus musculus (house mouse)	Spint1	NP_058603.2
Rattus norvegicus (Norway rat)	Spint1	NP_001004265.2
Danio rerio (zebrafish)	spint1a	NP_998317.1
Danio rerio (zebrafish)	spint1b	NP_001104693.1
Bos taurus (cattle)	SPINT1	NP_001039832.1

Gene Ontology
Bibliography

Related articles in PubMed

A Grhl2-dependent gene network controls trophoblast branching morphogenesis.
Walentin K, Hinze C, Werth M, Haase N, Varma S, Morell R, Aue A, P?tschke E, Warburton D, Qiu A, Barasch J, Purf?rst B, Dieterich C, Popova E, Bader M, Dechend R, Staff AC, Yurtdas ZY, Kilic E, Schmidt-Ott KM
Development (Cambridge, England)142(6)1125-36(2015 Mar)

Hepatocyte growth factor activator inhibitor-1 is induced by bone morphogenetic proteins and regulates proliferation and cell fate of neural progenitor cells.
Koivuniemi R, M?kel? J, Hokkanen ME, Bruelle C, Ho TH, Ola R, Korhonen L, Schr?der J, Kataoka H, Lindholm D
PloS one8(2)e56117(2013)

Persistent elevation of hepatocyte growth factor activator inhibitors in cholangiopathies affects liver fibrosis and differentiation.
Huang HP, Chang MH, Chen YT, Hsu HY, Chiang CL, Cheng TS, Wu YM, Wu MZ, Hsu YC, Shen CC, Lee CN, Chuang YH, Hong CL, Jeng YM, Chen PH, Chen HL, Lee MS
Hepatology (Baltimore, Md.)55(1)161-72(2012 Jan)

Identification of the matriptase second CUB domain as the secondary site for interaction with hepatocyte growth factor activator inhibitor type-1.
Inouye K, Tsuzuki S, Yasumoto M, Kojima K, Mochida S, Fushiki T
The Journal of biological chemistry285(43)33394-403(2010 Oct)

Regulation of cell surface protease matriptase by HAI2 is essential for placental development, neural tube closure and embryonic survival in mice.
Szabo R, Hobson JP, Christoph K, Kosa P, List K, Bugge TH
Development (Cambridge, England)136(15)2653-63(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

HAI-1 and HAI-2 influence proliferation and cell fate of NPCs and their expression levels are linked to BMP sign
Title: Hepatocyte growth factor activator inhibitor-1 is induced by bone morphogenetic proteins and regulates proliferation and cell fate of neural progenitor cells.

Identification of the matriptase second CUB domain as the secondary site for interaction with hepatocyte growth factor activator inhibitor type-1.
Title: Identification of the matriptase second CUB domain as the secondary site for interaction with hepatocyte growth factor activator inhibitor type-1.

The N-terminal domain and Kunitz domain II decreased the association rate between Kunitz domain I and matriptase, whereas the internal domain increased this rate.
Title: Roles of functional and structural domains of hepatocyte growth factor activator inhibitor type 1 in the inhibition of matriptase.

The following Spint1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spint1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10297	NM_001004265.2 Latest version!	Rattus norvegicus serine peptidase inhibitor, Kunitz type 1 (Spint1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$300.30 $429.00
ORa10297	XM_006234764.2 Latest version!	Rattus norvegicus serine peptidase inhibitor, Kunitz type 1 (Spint1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$300.30 $429.00
ORa10297	XM_006234763.2 Latest version!	Rattus norvegicus serine peptidase inhibitor, Kunitz type 1 (Spint1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$300.30 $429.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spint1 ( NM_001004265.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001004265.2 Rattus norvegicus serine peptidase inhibitor, Kunitz type 1 (Spint1), mRNA.

Price & Availability↑

CloneID	ORa10297
Clone ID Related Accession (Same CDS sequence)	XM_006234763.2 , NM_001004265.2 , XM_006234764.2
Accession Version	NM_001004265.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Rattus norvegicus(Norway rat)
Product	kunitz-type protease inhibitor 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC097253.1. On Jun 12, 2005 this sequence version replaced NM_001004265.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC097253.1, AB154834.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGCCC GCCGCCTGGC GCAGGCCAGC ATCTCTACGG TCTGCGTATG GCTCCTTTGC 
GCGCTTGGTC TCCAGGGCAC TGAGACCGAG CTGCCGTCCG CACCCCCTGA GTTTTCCGGG 
GGAGCCGCCT GCTTGAACCG CTTTACCAGC GGGGTGCCGG CTTTTGTGTT GGACACCGAA 
GCTTCAGTCA GCAACGGGGC CACCTTCCTG GGTTCCCCGA CGGTGCACCG CGGCTGGGAC 
TGCGTGCGTG CCTGCTGCAC CACCCAAAAC TGTAACCTGG CGCTGGTGGA GCTGCAGCCG 
GACGGAGGCG AGGACGCCAT CTCTGCCTGC TTCCTCATGA ACTGCCTGTA TGAGCAGAAC 
TTCGTGTGCA AATTCGCTCC GAAGGACGGC TTCATCAACT ATCTTACCCA AGATCTTTAC 
AACTCCTACC GCGAGCTGCG AACTCGAGGC TTTGGAGGTT CCCGGATCCC TCGAATCTGG 
ATGGGCATAG ACTTGAAGGT GCAGCTGCAG AAGCCCCTGG TACTGAGAGA TGCAGACAAC 
ACAGACTGGC ATCTACTGCA AGGTGACCAA GATGTCAGTG TTAAGAGAAA CCATCCAGAA 
GAAGTTGAGC TGTGGGGACT TAAGGAGGGA ACCTACCTGT TCCAACTGAC AAGAACCGAC 
TCAGACCAGC TAGAGGAAAC AGTCAACCTG ACTATCACCG TGCTAACTGC CGAGCAGACG 
GAAGATTATT GCCTCGCATC CTACAAGGTG GGCCGCTGCC GGGGCTCCTT CCCACGCTGG 
TACTATGACC CCAAGGAACA GATCTGCAAA AGTTTCACTT TTGGAGGTTG CCTAGGCAAC 
AAGAACAACT ACCTTCGTGA GGAAGAGTGC ATGCTAGCCT GCAAGGATGT GAAAGGAATC 
TCACCTAAAA GGCATCATCC AGTGTGCACC GGCAGCTGCC ATAGCACGCA GTTCCAGTGC 
AGCAATGGCT GCTGTATCGA CGGCTTCCTG GAGTGTGATG ACACGCCTGA CTGCCCTGAT 
GGCTCCGATG AGGCCACTTG TGAAAAATAT AGCAGCGGCT TTGATGAACT TCAGAGTATC 
CACTTCCTCA GTGACAAAGG GTACTGTGCA GAGCTGCCAG ACACTGGATT TTGCAAGGAG 
AACATCCCAC GCTGGTATTA CAACCCATTC AGTGAACGCT GTGCCCGCTT CACCTATGGT 
GGTTGCTACG GGAACAAGAA CAACTTTGAG AAGGAACAGC AGTGTCTTGA GTCCTGTCGT 
GGCATCTCCA AGAAGGATGT GTTCGGTCTT CGGAGGGAAA GCTCCGTTCC CAGTGCAGGC 
TCTGTGGAGG TAGCTATTGC TGTATTGCTG GCCGTCTGCA TCATAGTGGT CTTGACCATC 
CTGGGCTACT GTTTCTTCAA GAACCAAAAA AAGAACTTCC ACAGCCCCCT GCACCAACCA 
CCTCCCACAC CAGCCAGCTC CACTGTTTCC ACCACTGAGG ACACAGAGCA CCTGGTCTAT 
AATCACACAA CCCAGCCTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004265.2
CDS	228..1751
Translation	MAARRLAQASISTVCVWLLCALGLQGTETELPSAPPEFSGGAACLNRFTSGVPAFVLDTEASVSNGATFLGSPTVHRGWDCVRACCTTQNCNLALVELQPDGGEDAISACFLMNCLYEQNFVCKFAPKDGFINYLTQDLYNSYRELRTRGFGGSRIPRIWMGIDLKVQLQKPLVLRDADNTDWHLLQGDQDVSVKRNHPEEVELWGLKEGTYLFQLTRTDSDQLEETVNLTITVLTAEQTEDYCLASYKVGRCRGSFPRWYYDPKEQICKSFTFGGCLGNKNNYLREEECMLACKDVKGISPKRHHPVCTGSCHSTQFQCSNGCCIDGFLECDDTPDCPDGSDEATCEKYSSGFDELQSIHFLSDKGYCAELPDTGFCKENIPRWYYNPFSERCARFTYGGCYGNKNNFEKEQQCLESCRGISKKDVFGLRRESSVPSAGSVEVAIAVLLAVCIIVVLTILGYCFFKNQKKNFHSPLHQPPPTPASSTVSTTEDTEHLVYNHTTQPL

Target ORF information:

RefSeq Version	NM_001004265.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serine peptidase inhibitor, Kunitz type 1 (Spint1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004265.2

ORF Insert Sequence:

ATGGCGGCCC GCCGCCTGGC GCAGGCCAGC ATCTCTACGG TCTGCGTATG GCTCCTTTGC 
GCGCTTGGTC TCCAGGGCAC TGAGACCGAG CTGCCGTCCG CACCCCCTGA GTTTTCCGGG 
GGAGCCGCCT GCTTGAACCG CTTTACCAGC GGGGTGCCGG CTTTTGTGTT GGACACCGAA 
GCTTCAGTCA GCAACGGGGC CACCTTCCTG GGTTCCCCGA CGGTGCACCG CGGCTGGGAC 
TGCGTGCGTG CCTGCTGCAC CACCCAAAAC TGTAACCTGG CGCTGGTGGA GCTGCAGCCG 
GACGGAGGCG AGGACGCCAT CTCTGCCTGC TTCCTCATGA ACTGCCTGTA TGAGCAGAAC 
TTCGTGTGCA AATTCGCTCC GAAGGACGGC TTCATCAACT ATCTTACCCA AGATCTTTAC 
AACTCCTACC GCGAGCTGCG AACTCGAGGC TTTGGAGGTT CCCGGATCCC TCGAATCTGG 
ATGGGCATAG ACTTGAAGGT GCAGCTGCAG AAGCCCCTGG TACTGAGAGA TGCAGACAAC 
ACAGACTGGC ATCTACTGCA AGGTGACCAA GATGTCAGTG TTAAGAGAAA CCATCCAGAA 
GAAGTTGAGC TGTGGGGACT TAAGGAGGGA ACCTACCTGT TCCAACTGAC AAGAACCGAC 
TCAGACCAGC TAGAGGAAAC AGTCAACCTG ACTATCACCG TGCTAACTGC CGAGCAGACG 
GAAGATTATT GCCTCGCATC CTACAAGGTG GGCCGCTGCC GGGGCTCCTT CCCACGCTGG 
TACTATGACC CCAAGGAACA GATCTGCAAA AGTTTCACTT TTGGAGGTTG CCTAGGCAAC 
AAGAACAACT ACCTTCGTGA GGAAGAGTGC ATGCTAGCCT GCAAGGATGT GAAAGGAATC 
TCACCTAAAA GGCATCATCC AGTGTGCACC GGCAGCTGCC ATAGCACGCA GTTCCAGTGC 
AGCAATGGCT GCTGTATCGA CGGCTTCCTG GAGTGTGATG ACACGCCTGA CTGCCCTGAT 
GGCTCCGATG AGGCCACTTG TGAAAAATAT AGCAGCGGCT TTGATGAACT TCAGAGTATC 
CACTTCCTCA GTGACAAAGG GTACTGTGCA GAGCTGCCAG ACACTGGATT TTGCAAGGAG 
AACATCCCAC GCTGGTATTA CAACCCATTC AGTGAACGCT GTGCCCGCTT CACCTATGGT 
GGTTGCTACG GGAACAAGAA CAACTTTGAG AAGGAACAGC AGTGTCTTGA GTCCTGTCGT 
GGCATCTCCA AGAAGGATGT GTTCGGTCTT CGGAGGGAAA GCTCCGTTCC CAGTGCAGGC 
TCTGTGGAGG TAGCTATTGC TGTATTGCTG GCCGTCTGCA TCATAGTGGT CTTGACCATC 
CTGGGCTACT GTTTCTTCAA GAACCAAAAA AAGAACTTCC ACAGCCCCCT GCACCAACCA 
CCTCCCACAC CAGCCAGCTC CACTGTTTCC ACCACTGAGG ACACAGAGCA CCTGGTCTAT 
AATCACACAA CCCAGCCTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spint1 ( XM_006234763.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234825.1 Rattus norvegicus serine peptidase inhibitor, Kunitz type 1 (Spint1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa10297
Clone ID Related Accession (Same CDS sequence)	XM_006234763.2 , NM_001004265.2 , XM_006234764.2
Accession Version	XM_006234763.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kunitz-type protease inhibitor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234763.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC GCCGCCTGGC GCAGGCCAGC ATCTCTACGG TCTGCGTATG GCTCCTTTGC 
GCGCTTGGTC TCCAGGGCAC TGAGACCGAG CTGCCGTCCG CACCCCCTGA GTTTTCCGGG 
GGAGCCGCCT GCTTGAACCG CTTTACCAGC GGGGTGCCGG CTTTTGTGTT GGACACCGAA 
GCTTCAGTCA GCAACGGGGC CACCTTCCTG GGTTCCCCGA CGGTGCACCG CGGCTGGGAC 
TGCGTGCGTG CCTGCTGCAC CACCCAAAAC TGTAACCTGG CGCTGGTGGA GCTGCAGCCG 
GACGGAGGCG AGGACGCCAT CTCTGCCTGC TTCCTCATGA ACTGCCTGTA TGAGCAGAAC 
TTCGTGTGCA AATTCGCTCC GAAGGACGGC TTCATCAACT ATCTTACCCA AGATCTTTAC 
AACTCCTACC GCGAGCTGCG AACTCGAGGC TTTGGAGGTT CCCGGATCCC TCGAATCTGG 
ATGGGCATAG ACTTGAAGGT GCAGCTGCAG AAGCCCCTGG TACTGAGAGA TGCAGACAAC 
ACAGACTGGC ATCTACTGCA AGGTGACCAA GATGTCAGTG TTAAGAGAAA CCATCCAGAA 
GAAGTTGAGC TGTGGGGACT TAAGGAGGGA ACCTACCTGT TCCAACTGAC AAGAACCGAC 
TCAGACCAGC TAGAGGAAAC AGTCAACCTG ACTATCACCG TGCTAACTGC CGAGCAGACG 
GAAGATTATT GCCTCGCATC CTACAAGGTG GGCCGCTGCC GGGGCTCCTT CCCACGCTGG 
TACTATGACC CCAAGGAACA GATCTGCAAA AGTTTCACTT TTGGAGGTTG CCTAGGCAAC 
AAGAACAACT ACCTTCGTGA GGAAGAGTGC ATGCTAGCCT GCAAGGATGT GAAAGGAATC 
TCACCTAAAA GGCATCATCC AGTGTGCACC GGCAGCTGCC ATAGCACGCA GTTCCAGTGC 
AGCAATGGCT GCTGTATCGA CGGCTTCCTG GAGTGTGATG ACACGCCTGA CTGCCCTGAT 
GGCTCCGATG AGGCCACTTG TGAAAAATAT AGCAGCGGCT TTGATGAACT TCAGAGTATC 
CACTTCCTCA GTGACAAAGG GTACTGTGCA GAGCTGCCAG ACACTGGATT TTGCAAGGAG 
AACATCCCAC GCTGGTATTA CAACCCATTC AGTGAACGCT GTGCCCGCTT CACCTATGGT 
GGTTGCTACG GGAACAAGAA CAACTTTGAG AAGGAACAGC AGTGTCTTGA GTCCTGTCGT 
GGCATCTCCA AGAAGGATGT GTTCGGTCTT CGGAGGGAAA GCTCCGTTCC CAGTGCAGGC 
TCTGTGGAGG TAGCTATTGC TGTATTGCTG GCCGTCTGCA TCATAGTGGT CTTGACCATC 
CTGGGCTACT GTTTCTTCAA GAACCAAAAA AAGAACTTCC ACAGCCCCCT GCACCAACCA 
CCTCCCACAC CAGCCAGCTC CACTGTTTCC ACCACTGAGG ACACAGAGCA CCTGGTCTAT 
AATCACACAA CCCAGCCTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234825.1
CDS	190..1713
Translation	MAARRLAQASISTVCVWLLCALGLQGTETELPSAPPEFSGGAACLNRFTSGVPAFVLDTEASVSNGATFLGSPTVHRGWDCVRACCTTQNCNLALVELQPDGGEDAISACFLMNCLYEQNFVCKFAPKDGFINYLTQDLYNSYRELRTRGFGGSRIPRIWMGIDLKVQLQKPLVLRDADNTDWHLLQGDQDVSVKRNHPEEVELWGLKEGTYLFQLTRTDSDQLEETVNLTITVLTAEQTEDYCLASYKVGRCRGSFPRWYYDPKEQICKSFTFGGCLGNKNNYLREEECMLACKDVKGISPKRHHPVCTGSCHSTQFQCSNGCCIDGFLECDDTPDCPDGSDEATCEKYSSGFDELQSIHFLSDKGYCAELPDTGFCKENIPRWYYNPFSERCARFTYGGCYGNKNNFEKEQQCLESCRGISKKDVFGLRRESSVPSAGSVEVAIAVLLAVCIIVVLTILGYCFFKNQKKNFHSPLHQPPPTPASSTVSTTEDTEHLVYNHTTQPL

Target ORF information:

RefSeq Version	XM_006234763.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serine peptidase inhibitor, Kunitz type 1 (Spint1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234763.2

ORF Insert Sequence:

ATGGCGGCCC GCCGCCTGGC GCAGGCCAGC ATCTCTACGG TCTGCGTATG GCTCCTTTGC 
GCGCTTGGTC TCCAGGGCAC TGAGACCGAG CTGCCGTCCG CACCCCCTGA GTTTTCCGGG 
GGAGCCGCCT GCTTGAACCG CTTTACCAGC GGGGTGCCGG CTTTTGTGTT GGACACCGAA 
GCTTCAGTCA GCAACGGGGC CACCTTCCTG GGTTCCCCGA CGGTGCACCG CGGCTGGGAC 
TGCGTGCGTG CCTGCTGCAC CACCCAAAAC TGTAACCTGG CGCTGGTGGA GCTGCAGCCG 
GACGGAGGCG AGGACGCCAT CTCTGCCTGC TTCCTCATGA ACTGCCTGTA TGAGCAGAAC 
TTCGTGTGCA AATTCGCTCC GAAGGACGGC TTCATCAACT ATCTTACCCA AGATCTTTAC 
AACTCCTACC GCGAGCTGCG AACTCGAGGC TTTGGAGGTT CCCGGATCCC TCGAATCTGG 
ATGGGCATAG ACTTGAAGGT GCAGCTGCAG AAGCCCCTGG TACTGAGAGA TGCAGACAAC 
ACAGACTGGC ATCTACTGCA AGGTGACCAA GATGTCAGTG TTAAGAGAAA CCATCCAGAA 
GAAGTTGAGC TGTGGGGACT TAAGGAGGGA ACCTACCTGT TCCAACTGAC AAGAACCGAC 
TCAGACCAGC TAGAGGAAAC AGTCAACCTG ACTATCACCG TGCTAACTGC CGAGCAGACG 
GAAGATTATT GCCTCGCATC CTACAAGGTG GGCCGCTGCC GGGGCTCCTT CCCACGCTGG 
TACTATGACC CCAAGGAACA GATCTGCAAA AGTTTCACTT TTGGAGGTTG CCTAGGCAAC 
AAGAACAACT ACCTTCGTGA GGAAGAGTGC ATGCTAGCCT GCAAGGATGT GAAAGGAATC 
TCACCTAAAA GGCATCATCC AGTGTGCACC GGCAGCTGCC ATAGCACGCA GTTCCAGTGC 
AGCAATGGCT GCTGTATCGA CGGCTTCCTG GAGTGTGATG ACACGCCTGA CTGCCCTGAT 
GGCTCCGATG AGGCCACTTG TGAAAAATAT AGCAGCGGCT TTGATGAACT TCAGAGTATC 
CACTTCCTCA GTGACAAAGG GTACTGTGCA GAGCTGCCAG ACACTGGATT TTGCAAGGAG 
AACATCCCAC GCTGGTATTA CAACCCATTC AGTGAACGCT GTGCCCGCTT CACCTATGGT 
GGTTGCTACG GGAACAAGAA CAACTTTGAG AAGGAACAGC AGTGTCTTGA GTCCTGTCGT 
GGCATCTCCA AGAAGGATGT GTTCGGTCTT CGGAGGGAAA GCTCCGTTCC CAGTGCAGGC 
TCTGTGGAGG TAGCTATTGC TGTATTGCTG GCCGTCTGCA TCATAGTGGT CTTGACCATC 
CTGGGCTACT GTTTCTTCAA GAACCAAAAA AAGAACTTCC ACAGCCCCCT GCACCAACCA 
CCTCCCACAC CAGCCAGCTC CACTGTTTCC ACCACTGAGG ACACAGAGCA CCTGGTCTAT 
AATCACACAA CCCAGCCTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spint1 ( XM_006234764.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234826.1 Rattus norvegicus serine peptidase inhibitor, Kunitz type 1 (Spint1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa10297
Clone ID Related Accession (Same CDS sequence)	XM_006234763.2 , NM_001004265.2 , XM_006234764.2
Accession Version	XM_006234764.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	kunitz-type protease inhibitor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006234764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCCC GCCGCCTGGC GCAGGCCAGC ATCTCTACGG TCTGCGTATG GCTCCTTTGC 
GCGCTTGGTC TCCAGGGCAC TGAGACCGAG CTGCCGTCCG CACCCCCTGA GTTTTCCGGG 
GGAGCCGCCT GCTTGAACCG CTTTACCAGC GGGGTGCCGG CTTTTGTGTT GGACACCGAA 
GCTTCAGTCA GCAACGGGGC CACCTTCCTG GGTTCCCCGA CGGTGCACCG CGGCTGGGAC 
TGCGTGCGTG CCTGCTGCAC CACCCAAAAC TGTAACCTGG CGCTGGTGGA GCTGCAGCCG 
GACGGAGGCG AGGACGCCAT CTCTGCCTGC TTCCTCATGA ACTGCCTGTA TGAGCAGAAC 
TTCGTGTGCA AATTCGCTCC GAAGGACGGC TTCATCAACT ATCTTACCCA AGATCTTTAC 
AACTCCTACC GCGAGCTGCG AACTCGAGGC TTTGGAGGTT CCCGGATCCC TCGAATCTGG 
ATGGGCATAG ACTTGAAGGT GCAGCTGCAG AAGCCCCTGG TACTGAGAGA TGCAGACAAC 
ACAGACTGGC ATCTACTGCA AGGTGACCAA GATGTCAGTG TTAAGAGAAA CCATCCAGAA 
GAAGTTGAGC TGTGGGGACT TAAGGAGGGA ACCTACCTGT TCCAACTGAC AAGAACCGAC 
TCAGACCAGC TAGAGGAAAC AGTCAACCTG ACTATCACCG TGCTAACTGC CGAGCAGACG 
GAAGATTATT GCCTCGCATC CTACAAGGTG GGCCGCTGCC GGGGCTCCTT CCCACGCTGG 
TACTATGACC CCAAGGAACA GATCTGCAAA AGTTTCACTT TTGGAGGTTG CCTAGGCAAC 
AAGAACAACT ACCTTCGTGA GGAAGAGTGC ATGCTAGCCT GCAAGGATGT GAAAGGAATC 
TCACCTAAAA GGCATCATCC AGTGTGCACC GGCAGCTGCC ATAGCACGCA GTTCCAGTGC 
AGCAATGGCT GCTGTATCGA CGGCTTCCTG GAGTGTGATG ACACGCCTGA CTGCCCTGAT 
GGCTCCGATG AGGCCACTTG TGAAAAATAT AGCAGCGGCT TTGATGAACT TCAGAGTATC 
CACTTCCTCA GTGACAAAGG GTACTGTGCA GAGCTGCCAG ACACTGGATT TTGCAAGGAG 
AACATCCCAC GCTGGTATTA CAACCCATTC AGTGAACGCT GTGCCCGCTT CACCTATGGT 
GGTTGCTACG GGAACAAGAA CAACTTTGAG AAGGAACAGC AGTGTCTTGA GTCCTGTCGT 
GGCATCTCCA AGAAGGATGT GTTCGGTCTT CGGAGGGAAA GCTCCGTTCC CAGTGCAGGC 
TCTGTGGAGG TAGCTATTGC TGTATTGCTG GCCGTCTGCA TCATAGTGGT CTTGACCATC 
CTGGGCTACT GTTTCTTCAA GAACCAAAAA AAGAACTTCC ACAGCCCCCT GCACCAACCA 
CCTCCCACAC CAGCCAGCTC CACTGTTTCC ACCACTGAGG ACACAGAGCA CCTGGTCTAT 
AATCACACAA CCCAGCCTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234826.1
CDS	166..1689
Translation	MAARRLAQASISTVCVWLLCALGLQGTETELPSAPPEFSGGAACLNRFTSGVPAFVLDTEASVSNGATFLGSPTVHRGWDCVRACCTTQNCNLALVELQPDGGEDAISACFLMNCLYEQNFVCKFAPKDGFINYLTQDLYNSYRELRTRGFGGSRIPRIWMGIDLKVQLQKPLVLRDADNTDWHLLQGDQDVSVKRNHPEEVELWGLKEGTYLFQLTRTDSDQLEETVNLTITVLTAEQTEDYCLASYKVGRCRGSFPRWYYDPKEQICKSFTFGGCLGNKNNYLREEECMLACKDVKGISPKRHHPVCTGSCHSTQFQCSNGCCIDGFLECDDTPDCPDGSDEATCEKYSSGFDELQSIHFLSDKGYCAELPDTGFCKENIPRWYYNPFSERCARFTYGGCYGNKNNFEKEQQCLESCRGISKKDVFGLRRESSVPSAGSVEVAIAVLLAVCIIVVLTILGYCFFKNQKKNFHSPLHQPPPTPASSTVSTTEDTEHLVYNHTTQPL

Target ORF information:

RefSeq Version	XM_006234764.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus serine peptidase inhibitor, Kunitz type 1 (Spint1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234764.2

ORF Insert Sequence:

ATGGCGGCCC GCCGCCTGGC GCAGGCCAGC ATCTCTACGG TCTGCGTATG GCTCCTTTGC 
GCGCTTGGTC TCCAGGGCAC TGAGACCGAG CTGCCGTCCG CACCCCCTGA GTTTTCCGGG 
GGAGCCGCCT GCTTGAACCG CTTTACCAGC GGGGTGCCGG CTTTTGTGTT GGACACCGAA 
GCTTCAGTCA GCAACGGGGC CACCTTCCTG GGTTCCCCGA CGGTGCACCG CGGCTGGGAC 
TGCGTGCGTG CCTGCTGCAC CACCCAAAAC TGTAACCTGG CGCTGGTGGA GCTGCAGCCG 
GACGGAGGCG AGGACGCCAT CTCTGCCTGC TTCCTCATGA ACTGCCTGTA TGAGCAGAAC 
TTCGTGTGCA AATTCGCTCC GAAGGACGGC TTCATCAACT ATCTTACCCA AGATCTTTAC 
AACTCCTACC GCGAGCTGCG AACTCGAGGC TTTGGAGGTT CCCGGATCCC TCGAATCTGG 
ATGGGCATAG ACTTGAAGGT GCAGCTGCAG AAGCCCCTGG TACTGAGAGA TGCAGACAAC 
ACAGACTGGC ATCTACTGCA AGGTGACCAA GATGTCAGTG TTAAGAGAAA CCATCCAGAA 
GAAGTTGAGC TGTGGGGACT TAAGGAGGGA ACCTACCTGT TCCAACTGAC AAGAACCGAC 
TCAGACCAGC TAGAGGAAAC AGTCAACCTG ACTATCACCG TGCTAACTGC CGAGCAGACG 
GAAGATTATT GCCTCGCATC CTACAAGGTG GGCCGCTGCC GGGGCTCCTT CCCACGCTGG 
TACTATGACC CCAAGGAACA GATCTGCAAA AGTTTCACTT TTGGAGGTTG CCTAGGCAAC 
AAGAACAACT ACCTTCGTGA GGAAGAGTGC ATGCTAGCCT GCAAGGATGT GAAAGGAATC 
TCACCTAAAA GGCATCATCC AGTGTGCACC GGCAGCTGCC ATAGCACGCA GTTCCAGTGC 
AGCAATGGCT GCTGTATCGA CGGCTTCCTG GAGTGTGATG ACACGCCTGA CTGCCCTGAT 
GGCTCCGATG AGGCCACTTG TGAAAAATAT AGCAGCGGCT TTGATGAACT TCAGAGTATC 
CACTTCCTCA GTGACAAAGG GTACTGTGCA GAGCTGCCAG ACACTGGATT TTGCAAGGAG 
AACATCCCAC GCTGGTATTA CAACCCATTC AGTGAACGCT GTGCCCGCTT CACCTATGGT 
GGTTGCTACG GGAACAAGAA CAACTTTGAG AAGGAACAGC AGTGTCTTGA GTCCTGTCGT 
GGCATCTCCA AGAAGGATGT GTTCGGTCTT CGGAGGGAAA GCTCCGTTCC CAGTGCAGGC 
TCTGTGGAGG TAGCTATTGC TGTATTGCTG GCCGTCTGCA TCATAGTGGT CTTGACCATC 
CTGGGCTACT GTTTCTTCAA GAACCAAAAA AAGAACTTCC ACAGCCCCCT GCACCAACCA 
CCTCCCACAC CAGCCAGCTC CACTGTTTCC ACCACTGAGG ACACAGAGCA CCTGGTCTAT 
AATCACACAA CCCAGCCTCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Grhl2-dependent gene network controls trophoblast branching morphogenesis.
Development (Cambridge, England)142(6)1125-36(2015 Mar)
Walentin K,Hinze C,Werth M,Haase N,Varma S,Morell R,Aue A,P?tschke E,Warburton D,Qiu A,Barasch J,Purf?rst B,Dieterich C,Popova E,Bader M,Dechend R,Staff AC,Yurtdas ZY,Kilic E,Schmidt-Ott KM

Hepatocyte growth factor activator inhibitor-1 is induced by bone morphogenetic proteins and regulates proliferation and cell fate of neural progenitor cells.
PloS one8(2)e56117(2013)
Koivuniemi R,M?kel? J,Hokkanen ME,Bruelle C,Ho TH,Ola R,Korhonen L,Schr?der J,Kataoka H,Lindholm D

Persistent elevation of hepatocyte growth factor activator inhibitors in cholangiopathies affects liver fibrosis and differentiation.
Hepatology (Baltimore, Md.)55(1)161-72(2012 Jan)
Huang HP,Chang MH,Chen YT,Hsu HY,Chiang CL,Cheng TS,Wu YM,Wu MZ,Hsu YC,Shen CC,Lee CN,Chuang YH,Hong CL,Jeng YM,Chen PH,Chen HL,Lee MS

Identification of the matriptase second CUB domain as the secondary site for interaction with hepatocyte growth factor activator inhibitor type-1.
The Journal of biological chemistry285(43)33394-403(2010 Oct)
Inouye K,Tsuzuki S,Yasumoto M,Kojima K,Mochida S,Fushiki T

Regulation of cell surface protease matriptase by HAI2 is essential for placental development, neural tube closure and embryonic survival in mice.
Development (Cambridge, England)136(15)2653-63(2009 Aug)
Szabo R,Hobson JP,Christoph K,Kosa P,List K,Bugge TH