Aph1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Aph1b
Entrez Gene ID	300802
Full Name	aph-1 homolog B, gamma secretase subunit
Synonyms	APH-1B'
General protein information	Preferred Names aph-1 homolog B, gamma secretase subunit Names gamma-secretase subunit APH-1B APH1B gamma secretase subunit anterior pharynx defective 1 homolog B anterior pharynx defective 1b homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q24

mRNA and Protein(s)

mRNA	Protein	Name
NM_001047090.1	NP_001040555.1	gamma-secretase subunit APH-1B

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	RGD1559979	XP_003750580.1
Mus musculus (house mouse)	Aph1c	NP_080950.1
Rattus norvegicus (Norway rat)	Aph1b	NP_001040555.1

Gene Ontology
Bibliography

Related articles in PubMed

Determination of relative Notch1 and gamma-secretase-related gene expression in puromycin-treated microdissected rat kidneys.
Simic D, Simutis F, Euler C, Thurby C, Peden WM, Bunch RT, Pilcher G, Sanderson T, Van Vleet T
Gene expression16(1)39-47(2013)

Gene expression profiling in brain regions of a rat model displaying schizophrenia-related features.
Van Schijndel JE, Van Zweeden M, Van Loo KM, Martens GJ
Behavioural brain research207(2)476-9(2010 Mar)

Cell and molecular biology of Notch.
Fi?za UM, Arias AM
The Journal of endocrinology194(3)459-74(2007 Sep)

Ontogenic reduction of Aph-1b mRNA and gamma-secretase activity in rats with a complex neurodevelopmental phenotype.
Coolen MW, van Loo KM, Ellenbroek BA, Cools AR, Martens GJ
Molecular psychiatry11(8)787-93(2006 Aug)

Reduced Aph-1b expression causes tissue- and substrate-specific changes in gamma-secretase activity in rats with a complex phenotype.
Coolen MW, van Loo KM, van Bakel NN, Ellenbroek BA, Cools AR, Martens GJ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology20(1)175-7(2006 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Notch ligand binding leads to gamma-secretase-mediated proteolytic cleavage of the Notch receptor releasing the Notch intracellular domain, resulting in its subsequent translocation into the nucleus and gene expression regulation
Title: Determination of relative Notch1 and gamma-secretase-related gene expression in puromycin-treated microdissected rat kidneys.

Aph1b and KCnIP1 are significantly differentially expressed in a number of brain tissues of a schizophrenia model; KCnIP1 is an attractive novel candidate gene that may play a role in the complex phenotype
Title: Gene expression profiling in brain regions of a rat model displaying schizophrenia-related features.

low levels of Aph-1b mRNA and gamma-secretase activity observed in the I/I and II/II rats during the entire developmental period may well underlie their complex phenotype
Title: Ontogenic reduction of Aph-1b mRNA and gamma-secretase activity in rats with a complex neurodevelopmental phenotype.

Pharmacogenetically bred apomorphine-susceptible Wistar rats have only one or two copies of the Aph-1b gene; in these rats, gamma-secretase activity is altered, leading to a complex phenotype.
Title: Reduced Aph-1b expression causes tissue- and substrate-specific changes in gamma-secretase activity in rats with a complex phenotype.

The following Aph1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Aph1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10005	NM_001047090.1 Latest version!	Rattus norvegicus aph-1 homolog B, gamma secretase subunit (Aph1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Aph1b ( NM_001047090.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001040555.1 Rattus norvegicus aph-1 homolog B, gamma secretase subunit (Aph1b), mRNA.

Price & Availability↑

CloneID	ORa10005
Clone ID Related Accession (Same CDS sequence)	NM_001047090.1
Accession Version	NM_001047090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	gamma-secretase subunit APH-1B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ672552.1. On or before Sep 7, 2006 this sequence version replaced XM_217185.4, XM_001076887.1. ##Evidence-Data-START## Transcript exon combination :: DQ672552.1, BC166592.1 [ECO:0000332] ##Evidence-Data-END##

ATGACAGCGC CCGTGTTCTT CGGTTGCGCC TTCATCGCCT TCGGGCCCGC GCTCGCTCTC 
TACCTCTTCA CCATCGCCAC CGACCCTTTG CGAGTCATCT TCCTCATCGC CGGTGCCTTC 
TTCTGGTTGG TGTCTCTGCT GCTTTCGTCT GTTTTCTGGT TCCTAGTGAG AGTCATCACT 
GACAACAGAG ATGGACCAGT ACAGAATTAC CTGCTCATCT TTGGAGTGTT GCTCTCTGTC 
TGTATCCAAG AGCTGTTCAG GCTCGCATAT TATAGGCTGT TAAAAAAAGC CAGCGAGGGT 
TTGAAAAGCA TAAACCCTGA GGAGACAGCA CCTTCGATGC GACTGTTGGC CTATGTTTCT 
GGCTTGGGCT TTGGAATCAT GAGTGGAGTG TTTTCCTTTG TGAACACCCT GTCTAATGCC 
TTGGGGCCAG GCACAGTGGG CATTCATGGT GATTCTCCTC AGTTCTTCCT TAATTCAGCT 
TTCATGACGC TGGTTATCAT AATGCTGCAC GTGTTCTGGG GCATCGTGTT TTTTGACGGC 
TGTGAGAAGA ATAAGTGGTA CATCCTCCTC ACAGTTCTCC TGACCCACCT GCTGGTGTCC 
ACCCAGACTT TACTAAGTCC TCACTATGAA GTGAATCTGG TGACAGCATA TATAATCATG 
GTGCTCATGG GCATCTGGGC ATTCTGTGTT GCTGGAGGCA GCCGCCGAAG CCTGAAACTC 
TGTCTGCTCT GCCAAGACAA GGACTTTCTT CTTTACAACC AGCGCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001040555.1
CDS	138..911
Translation	MTAPVFFGCAFIAFGPALALYLFTIATDPLRVIFLIAGAFFWLVSLLLSSVFWFLVRVITDNRDGPVQNYLLIFGVLLSVCIQELFRLAYYRLLKKASEGLKSINPEETAPSMRLLAYVSGLGFGIMSGVFSFVNTLSNALGPGTVGIHGDSPQFFLNSAFMTLVIIMLHVFWGIVFFDGCEKNKWYILLTVLLTHLLVSTQTLLSPHYEVNLVTAYIIMVLMGIWAFCVAGGSRRSLKLCLLCQDKDFLLYNQRSR

Target ORF information:

RefSeq Version	NM_001047090.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aph-1 homolog B, gamma secretase subunit (Aph1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001047090.1

ORF Insert Sequence:

ATGACAGCGC CCGTGTTCTT CGGTTGCGCC TTCATCGCCT TCGGGCCCGC GCTCGCTCTC 
TACCTCTTCA CCATCGCCAC CGACCCTTTG CGAGTCATCT TCCTCATCGC CGGTGCCTTC 
TTCTGGTTGG TGTCTCTGCT GCTTTCGTCT GTTTTCTGGT TCCTAGTGAG AGTCATCACT 
GACAACAGAG ATGGACCAGT ACAGAATTAC CTGCTCATCT TTGGAGTGTT GCTCTCTGTC 
TGTATCCAAG AGCTGTTCAG GCTCGCATAT TATAGGCTGT TAAAAAAAGC CAGCGAGGGT 
TTGAAAAGCA TAAACCCTGA GGAGACAGCA CCTTCGATGC GACTGTTGGC CTATGTTTCT 
GGCTTGGGCT TTGGAATCAT GAGTGGAGTG TTTTCCTTTG TGAACACCCT GTCTAATGCC 
TTGGGGCCAG GCACAGTGGG CATTCATGGT GATTCTCCTC AGTTCTTCCT TAATTCAGCT 
TTCATGACGC TGGTTATCAT AATGCTGCAC GTGTTCTGGG GCATCGTGTT TTTTGACGGC 
TGTGAGAAGA ATAAGTGGTA CATCCTCCTC ACAGTTCTCC TGACCCACCT GCTGGTGTCC 
ACCCAGACTT TACTAAGTCC TCACTATGAA GTGAATCTGG TGACAGCATA TATAATCATG 
GTGCTCATGG GCATCTGGGC ATTCTGTGTT GCTGGAGGCA GCCGCCGAAG CCTGAAACTC 
TGTCTGCTCT GCCAAGACAA GGACTTTCTT CTTTACAACC AGCGCTCCAG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Determination of relative Notch1 and gamma-secretase-related gene expression in puromycin-treated microdissected rat kidneys.
Gene expression16(1)39-47(2013)
Simic D,Simutis F,Euler C,Thurby C,Peden WM,Bunch RT,Pilcher G,Sanderson T,Van Vleet T

Gene expression profiling in brain regions of a rat model displaying schizophrenia-related features.
Behavioural brain research207(2)476-9(2010 Mar)
Van Schijndel JE,Van Zweeden M,Van Loo KM,Martens GJ

Cell and molecular biology of Notch.
The Journal of endocrinology194(3)459-74(2007 Sep)
Fi?za UM,Arias AM

Ontogenic reduction of Aph-1b mRNA and gamma-secretase activity in rats with a complex neurodevelopmental phenotype.
Molecular psychiatry11(8)787-93(2006 Aug)
Coolen MW,van Loo KM,Ellenbroek BA,Cools AR,Martens GJ

Reduced Aph-1b expression causes tissue- and substrate-specific changes in gamma-secretase activity in rats with a complex phenotype.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology20(1)175-7(2006 Jan)
Coolen MW,van Loo KM,van Bakel NN,Ellenbroek BA,Cools AR,Martens GJ