Dpagt1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dpagt1
Entrez Gene ID	300668
Full Name	dolichyl-phosphate N-acetylglucosaminephosphotransferase 1
Synonyms	H2afx
General protein information	Preferred Names dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 Names UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase dolichyl-phosphate (UDP-N-acetylglucosamine) N-acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase) dolichyl-phosphate (UDP-N-acetylglucosamine) acetylglucosaminephosphotransferase 1 (GlcNAc-1-P transferase)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_199388.1	NP_955420.1	UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
XM_006242921.3	XP_006242983.1	UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase isoform X1
XM_017595550.1	XP_017451039.1	UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	dpagt1	NP_001082880.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008131	XP_317328.3
Magnaporthe oryzae (rice blast fungus)	MGG_01559	XP_003714534.1
Pan troglodytes (chimpanzee)	DPAGT1	XP_001165129.1
Gallus gallus (chicken)	DPAGT1	XP_417845.2
Schizosaccharomyces pombe (fission yeast)	gpt2	NP_596244.1
Canis lupus familiaris (dog)	DPAGT1	XP_546489.3
Mus musculus (house mouse)	Dpagt1	NP_031901.2
Arabidopsis thaliana (thale cress)	AT3G57220	NP_191281.1
Xenopus tropicalis (tropical clawed frog)	LOC100487779	XP_002935051.2
	DPAGT1	XP_001102857.1
Rattus norvegicus (Norway rat)	Dpagt1	NP_955420.1
Eremothecium gossypii	AGOS_AFL037W	NP_985511.1
Homo sapiens (human)	DPAGT1	NP_001373.2
Bos taurus (cattle)	DPAGT1	NP_001015664.1
Drosophila melanogaster (fruit fly)	CG5287	NP_609608.1
Caenorhabditis elegans (roundworm)	CELE_Y60A3A.14	NP_507859.2
Saccharomyces cerevisiae (baker's yeast)	ALG7	NP_009802.3
Kluyveromyces lactis	KLLA0F06776g	XP_455387.1
Neurospora crassa	NCU10762	XP_001728541.1
Arabidopsis thaliana (thale cress)	GPT	NP_565950.1
Oryza sativa (rice)	Os07g0661100	NP_001060535.1

Gene Ontology
Bibliography

Related articles in PubMed

Overexpression of DPAGT1 leads to aberrant N-glycosylation of E-cadherin and cellular discohesion in oral cancer.
Nita-Lazar M, Noonan V, Rebustini I, Walker J, Menko AS, Kukuruzinska MA
Cancer research69(14)5673-80(2009 Jul)

Deficiency of UDP-GlcNAc:Dolichol Phosphate N-Acetylglucosamine-1 Phosphate Transferase (DPAGT1) causes a novel congenital disorder of Glycosylation Type Ij.
Wu X, Rush JS, Karaoglu D, Krasnewich D, Lubinsky MS, Waechter CJ, Gilmore R, Freeze HH
Human mutation22(2)144-50(2003 Aug)

Cloning and functional expression of the human GlcNAc-1-P transferase, the enzyme for the committed step of the dolichol cycle, by heterologous complementation in Saccharomyces cerevisiae.
Eckert V, Blank M, Mazhari-Tabrizi R, Mumberg D, Funk M, Schwarz RT
Glycobiology8(1)77-85(1998 Jan)

Effect of GTP on the dolichol pathway for protein glycosylation in rat liver microsomes.
Bossuyt X, Blanckaert N
The Biochemical journal296 ( Pt 3)633-7(1993 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dpagt1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dpagt1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05285	NM_199388.1 Latest version!	Rattus norvegicus dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 (Dpagt1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa31071	XM_006242921.3 Latest version!	Rattus norvegicus dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 (Dpagt1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa62374	XM_017595550.1 Latest version!	Rattus norvegicus dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 (Dpagt1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dpagt1 ( NM_199388.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_955420.1 Rattus norvegicus dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 (Dpagt1), mRNA.

Price & Availability↑

CloneID	ORa05285
Clone ID Related Accession (Same CDS sequence)	NM_199388.1
Accession Version	NM_199388.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC063184.1. On Jan 12, 2004 this sequence version replaced XM_236188.2. ##Evidence-Data-START## Transcript exon combination :: BC063184.1, FQ213783.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTGGGCCT TCCCGGAGTT ACCTCTGCCG CTGCCGCTGT TGGTGAATTT GATCGGATCG 
CTGTTGGGAT TTGTGGCTAC CGTCACCCTC ATCCCTGCCT TCCGTAGCCA CTTTATCGCC 
GCGCGTCTCT GTGGCCAGGA CCTCAACAAG CTCAGCCGGC AGCAGATCCC AGAGTCCCAG 
GGAGTGATCA GCGGTGCTGT TTTCCTTATC ATCCTCTTCT GCTTCATCCC TTTCCCTTTC 
CTGAACTGCT TTGTGGAGGA GCAGTGTAAG GCATTCCCCC ACCATGAATT TGTGGCCCTG 
ATAGGTGCCC TCCTTGCTAT CTGCTGCATG ATCTTCCTGG GTTTTGCTGA TGACGTACTC 
AATCTGCGAT GGCGTCATAA GCTGCTGCTC CCCACAGCTG CCTCACTACC TCTCCTCATG 
GTCTACTTCA CTAACTTTGG CAATACAACC ATTGTGGTGC CGAAGCCCTT CCGCTGGATT 
CTTGGCCTGC ATTTGGATTT GGGCATCCTG TACTATGTCT ACATGGGACT GCTTGCAGTG 
TTCTGTACCA ATGCCATCAA CATCCTAGCG GGAATTAATG GCCTAGAGGC TGGTCAATCA 
CTAGTCATCT CTGCTTCTAT TATTGTCTTC AACCTGGTGG AGCTGGAAGG TGATTATCGG 
GACGATCATG TCTTTTCCCT CTACTTCATG ATGCCATTTT TTTTTACCAC CTTGGGATTG 
CTGTACCATA ACTGGTACCC GTCTCAGGTG TTTGTGGGAG ACACCTTCTG TTATTTTGCT 
GGCATGACCT TTGCCGTGGT GGGAATCTTG GGACACTTCA GCAAGACCAT GCTGCTCTTC 
TTTATGCCAC AAGTATTCAA TTTCCTCTAC TCACTGCCTC AGCTCTTTCA GATCATCCCC 
TGCCCTCGAC ACCGTATGCC CAGACTCAAT ACGAAGACAG GCAAACTGGA GATGAGCTAT 
TCCAAGTTCA AGACCAAGAG CCTCTCTTTC TTGGGCACGT TTATTTTAAA GGTAGCAGAG 
AGCCTCCGGC TGGTGACAGT TCACCGAGGG GAGAGTGAGG ATGGTGCCTT CACTGAGTGT 
AACAACATGA CCCTCATCAA CTTGCTACTT AAAGTCTTTG GGCCTACACA TGAGAGAAAC 
CTCACACTGT TCCTGCTGCT CCTGCAGGTT CTGAGCAGCG CTGTCACCTT CTCCATTCGT 
TACCAGCTCG TCCGACTCTT CTATGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955420.1
CDS	127..1359
Translation	MWAFPELPLPLPLLVNLIGSLLGFVATVTLIPAFRSHFIAARLCGQDLNKLSRQQIPESQGVISGAVFLIILFCFIPFPFLNCFVEEQCKAFPHHEFVALIGALLAICCMIFLGFADDVLNLRWRHKLLLPTAASLPLLMVYFTNFGNTTIVVPKPFRWILGLHLDLGILYYVYMGLLAVFCTNAINILAGINGLEAGQSLVISASIIVFNLVELEGDYRDDHVFSLYFMMPFFFTTLGLLYHNWYPSQVFVGDTFCYFAGMTFAVVGILGHFSKTMLLFFMPQVFNFLYSLPQLFQIIPCPRHRMPRLNTKTGKLEMSYSKFKTKSLSFLGTFILKVAESLRLVTVHRGESEDGAFTECNNMTLINLLLKVFGPTHERNLTLFLLLLQVLSSAVTFSIRYQLVRLFYDV

Target ORF information:

RefSeq Version	NM_199388.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 (Dpagt1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199388.1

ORF Insert Sequence:

ATGTGGGCCT TCCCGGAGTT ACCTCTGCCG CTGCCGCTGT TGGTGAATTT GATCGGATCG 
CTGTTGGGAT TTGTGGCTAC CGTCACCCTC ATCCCTGCCT TCCGTAGCCA CTTTATCGCC 
GCGCGTCTCT GTGGCCAGGA CCTCAACAAG CTCAGCCGGC AGCAGATCCC AGAGTCCCAG 
GGAGTGATCA GCGGTGCTGT TTTCCTTATC ATCCTCTTCT GCTTCATCCC TTTCCCTTTC 
CTGAACTGCT TTGTGGAGGA GCAGTGTAAG GCATTCCCCC ACCATGAATT TGTGGCCCTG 
ATAGGTGCCC TCCTTGCTAT CTGCTGCATG ATCTTCCTGG GTTTTGCTGA TGACGTACTC 
AATCTGCGAT GGCGTCATAA GCTGCTGCTC CCCACAGCTG CCTCACTACC TCTCCTCATG 
GTCTACTTCA CTAACTTTGG CAATACAACC ATTGTGGTGC CGAAGCCCTT CCGCTGGATT 
CTTGGCCTGC ATTTGGATTT GGGCATCCTG TACTATGTCT ACATGGGACT GCTTGCAGTG 
TTCTGTACCA ATGCCATCAA CATCCTAGCG GGAATTAATG GCCTAGAGGC TGGTCAATCA 
CTAGTCATCT CTGCTTCTAT TATTGTCTTC AACCTGGTGG AGCTGGAAGG TGATTATCGG 
GACGATCATG TCTTTTCCCT CTACTTCATG ATGCCATTTT TTTTTACCAC CTTGGGATTG 
CTGTACCATA ACTGGTACCC GTCTCAGGTG TTTGTGGGAG ACACCTTCTG TTATTTTGCT 
GGCATGACCT TTGCCGTGGT GGGAATCTTG GGACACTTCA GCAAGACCAT GCTGCTCTTC 
TTTATGCCAC AAGTATTCAA TTTCCTCTAC TCACTGCCTC AGCTCTTTCA GATCATCCCC 
TGCCCTCGAC ACCGTATGCC CAGACTCAAT ACGAAGACAG GCAAACTGGA GATGAGCTAT 
TCCAAGTTCA AGACCAAGAG CCTCTCTTTC TTGGGCACGT TTATTTTAAA GGTAGCAGAG 
AGCCTCCGGC TGGTGACAGT TCACCGAGGG GAGAGTGAGG ATGGTGCCTT CACTGAGTGT 
AACAACATGA CCCTCATCAA CTTGCTACTT AAAGTCTTTG GGCCTACACA TGAGAGAAAC 
CTCACACTGT TCCTGCTGCT CCTGCAGGTT CTGAGCAGCG CTGTCACCTT CTCCATTCGT 
TACCAGCTCG TCCGACTCTT CTATGATGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpagt1 ( XM_006242921.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242983.1 Rattus norvegicus dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 (Dpagt1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa31071
Clone ID Related Accession (Same CDS sequence)	XM_006242921.3
Accession Version	XM_006242921.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242921.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCT TCCCGGAGTT ACCTCTGCCG CTGCCGCTGT TGGTGAATTT GATCGGATCG 
CTGTTGGGAT TTGTGGCTAC CGTCACCCTC ATCCCTGCCT TCCGTAGCCA CTTTATCGCC 
GCGCGTCTCT GTGGCCAGGA CCTCAACAAG CTCAGCCGGC AGCAGATCCC AGAGTCCCAG 
GGAGTGATCA GCGGTGCTGT TTTCCTTATC ATCCTCTTCT GCTTCATCCC TTTCCCTTTC 
CTGAACTGCT TTGTGGAGGA GCAGTGTAAG GCATTCCCCC ACCATGAATT TGTGGCCCTG 
ATAGGTGCCC TCCTTGCTAT CTGCTGCATG ATCTTCCTGG GTTTTGCTGA TGACGTACTC 
AATCTGCGAT GGCGTCATAA GCTGCTGCTC CCCACAGCTG CCTCACTACC TCTCCTCATG 
GTCTACTTCA CTAACTTTGG CAATACAACC ATTGTGGTGC CGAAGCCCTT CCGCTGGATT 
CTTGGCCTGC ATTTGGATTT GGGCATCCTG TACTATGTCT ACATGGGACT GCTTGCAGTG 
TTCTGTACCA ATGCCATCAA CATCCTAGCG GGAATTAATG GCCTAGAGGC TGGTCAATCA 
CTAGTCATCT CTGCTTCTAT TATTGTCTTC AACCTGGTGG AGCTGGAAGG TGATTATCGG 
GACGATCATG TCTTTTCCCT CTACTTCATG ATGCCATTTT TTTTTACCAC CTTGGGATTG 
CTGTACCATA ACTGGTACCC GTCTCAGGTG TTTGTGGGAG ACACCTTCTG TTATTTTGCT 
GGCATGACCT TTGCCGTGGT GGGAATCTTG GGACACTTCA GCAAGACCAT GCTGCTCTTC 
TTTATGCCAC AAGTATTCAA TTTCCTCTAC TCACTGCCTC AGCTCTTTCA GATCATCCCC 
TGCCCTCGAC ACCGTATGCC CAGACTCAAT ACGAAGACAG GCAAACTGGA GATGAGCTAT 
TCCAAGTTCA AGACCAAGAG CCTCTCTTTC TTGGGCACGT TTATTTTAAA GGTAACAAGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242983.1
CDS	474..1496
Translation	MWAFPELPLPLPLLVNLIGSLLGFVATVTLIPAFRSHFIAARLCGQDLNKLSRQQIPESQGVISGAVFLIILFCFIPFPFLNCFVEEQCKAFPHHEFVALIGALLAICCMIFLGFADDVLNLRWRHKLLLPTAASLPLLMVYFTNFGNTTIVVPKPFRWILGLHLDLGILYYVYMGLLAVFCTNAINILAGINGLEAGQSLVISASIIVFNLVELEGDYRDDHVFSLYFMMPFFFTTLGLLYHNWYPSQVFVGDTFCYFAGMTFAVVGILGHFSKTMLLFFMPQVFNFLYSLPQLFQIIPCPRHRMPRLNTKTGKLEMSYSKFKTKSLSFLGTFILKVTR

Target ORF information:

RefSeq Version	XM_006242921.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 (Dpagt1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242921.3

ORF Insert Sequence:

ATGTGGGCCT TCCCGGAGTT ACCTCTGCCG CTGCCGCTGT TGGTGAATTT GATCGGATCG 
CTGTTGGGAT TTGTGGCTAC CGTCACCCTC ATCCCTGCCT TCCGTAGCCA CTTTATCGCC 
GCGCGTCTCT GTGGCCAGGA CCTCAACAAG CTCAGCCGGC AGCAGATCCC AGAGTCCCAG 
GGAGTGATCA GCGGTGCTGT TTTCCTTATC ATCCTCTTCT GCTTCATCCC TTTCCCTTTC 
CTGAACTGCT TTGTGGAGGA GCAGTGTAAG GCATTCCCCC ACCATGAATT TGTGGCCCTG 
ATAGGTGCCC TCCTTGCTAT CTGCTGCATG ATCTTCCTGG GTTTTGCTGA TGACGTACTC 
AATCTGCGAT GGCGTCATAA GCTGCTGCTC CCCACAGCTG CCTCACTACC TCTCCTCATG 
GTCTACTTCA CTAACTTTGG CAATACAACC ATTGTGGTGC CGAAGCCCTT CCGCTGGATT 
CTTGGCCTGC ATTTGGATTT GGGCATCCTG TACTATGTCT ACATGGGACT GCTTGCAGTG 
TTCTGTACCA ATGCCATCAA CATCCTAGCG GGAATTAATG GCCTAGAGGC TGGTCAATCA 
CTAGTCATCT CTGCTTCTAT TATTGTCTTC AACCTGGTGG AGCTGGAAGG TGATTATCGG 
GACGATCATG TCTTTTCCCT CTACTTCATG ATGCCATTTT TTTTTACCAC CTTGGGATTG 
CTGTACCATA ACTGGTACCC GTCTCAGGTG TTTGTGGGAG ACACCTTCTG TTATTTTGCT 
GGCATGACCT TTGCCGTGGT GGGAATCTTG GGACACTTCA GCAAGACCAT GCTGCTCTTC 
TTTATGCCAC AAGTATTCAA TTTCCTCTAC TCACTGCCTC AGCTCTTTCA GATCATCCCC 
TGCCCTCGAC ACCGTATGCC CAGACTCAAT ACGAAGACAG GCAAACTGGA GATGAGCTAT 
TCCAAGTTCA AGACCAAGAG CCTCTCTTTC TTGGGCACGT TTATTTTAAA GGTAACAAGG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dpagt1 ( XM_017595550.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451039.1 Rattus norvegicus dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 (Dpagt1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa62374
Clone ID Related Accession (Same CDS sequence)	XM_017595550.1
Accession Version	XM_017595550.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCT TCCCGGAGTT ACCTCTGCCG CTGCCGCTGT TGGTGAATTT GATCGGATCG 
CTGTTGGGAT TTGTGGCTAC CGTCACCCTC ATCCCTGCCT TCCGTAGCCA CTTTATCGCC 
GCGCGTCTCT GTGGCCAGGA CCTCAACAAG CTCAGCCGGC AGCAGATCCC AGAGTCCCAG 
GGAGTGATCA GCGGTGCTGT TTTCCTTATC ATCCTCTTCT GCTTCATCCC TTTCCCTTTC 
CTGAACTGCT TTGTGGAGGA GCAGTGTAAG GCATTCCCCC ACCATGAATT TGTGGCCCTG 
ATAGGTGCCC TCCTTGCTAT CTGCTGCATG ATCTTCCTGG GTTTTGCTGA TGACGTACTC 
AATCTGCGAT GGCGTCATAA GCTGCTGCTC CCCACAGCTG CCTCACTACC TCTCCTCATG 
GTCTACTTCA CTAACTTTGG CAATACAACC ATTGTGGTGC CGAAGCCCTT CCGCTGGATT 
CTTGGCCTGC ATTTGGATTT GGGCATCCTG TACTATGTCT ACATGGGACT GCTTGCAGTG 
TTCTGTACCA ATGCCATCAA CATCCTAGCG GGAATTAATG GCCTAGAGGC TGGTCAATCA 
CTAGTCATCT CTGCTTCTAT TATTGTCTTC AACCTGGTGG AGCTGGAAGG TGATTATCGG 
GACGATCATG TCTTTTCCCT CTACTTCATG ATGCCATTTT TTTTTACCAC CTTGGGATTG 
CTGTACCATA ACTGGTACCC GTCTCAGGTG TTTGTGGGAG ACACCTTCTG TTATTTTGCT 
GGCATGACCT TTGCCGTGGT GGGAATCTTG GGACACTTCA GCAAGACCAT GCTGCTCTTC 
TTTATGCCAC AAGTATTCAA TTTCCTCTAC TCACTGCCTC AGCTCTTTCA GATCATCCCC 
TGCCCTCGAC ACCGTATGCC CAGACTCAAT ACGAAGACAG GCAAACTGGA GATGAGCTAT 
TCCAAGTTCA AGACCAAGAG CCTCTCTTTC TTGGGCACGT TTATTTTAAA GGTAACAAGG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451039.1
CDS	474..1496
Translation	MWAFPELPLPLPLLVNLIGSLLGFVATVTLIPAFRSHFIAARLCGQDLNKLSRQQIPESQGVISGAVFLIILFCFIPFPFLNCFVEEQCKAFPHHEFVALIGALLAICCMIFLGFADDVLNLRWRHKLLLPTAASLPLLMVYFTNFGNTTIVVPKPFRWILGLHLDLGILYYVYMGLLAVFCTNAINILAGINGLEAGQSLVISASIIVFNLVELEGDYRDDHVFSLYFMMPFFFTTLGLLYHNWYPSQVFVGDTFCYFAGMTFAVVGILGHFSKTMLLFFMPQVFNFLYSLPQLFQIIPCPRHRMPRLNTKTGKLEMSYSKFKTKSLSFLGTFILKVTR

Target ORF information:

RefSeq Version	XM_017595550.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus dolichyl-phosphate N-acetylglucosaminephosphotransferase 1 (Dpagt1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017595550.1

ORF Insert Sequence:

ATGTGGGCCT TCCCGGAGTT ACCTCTGCCG CTGCCGCTGT TGGTGAATTT GATCGGATCG 
CTGTTGGGAT TTGTGGCTAC CGTCACCCTC ATCCCTGCCT TCCGTAGCCA CTTTATCGCC 
GCGCGTCTCT GTGGCCAGGA CCTCAACAAG CTCAGCCGGC AGCAGATCCC AGAGTCCCAG 
GGAGTGATCA GCGGTGCTGT TTTCCTTATC ATCCTCTTCT GCTTCATCCC TTTCCCTTTC 
CTGAACTGCT TTGTGGAGGA GCAGTGTAAG GCATTCCCCC ACCATGAATT TGTGGCCCTG 
ATAGGTGCCC TCCTTGCTAT CTGCTGCATG ATCTTCCTGG GTTTTGCTGA TGACGTACTC 
AATCTGCGAT GGCGTCATAA GCTGCTGCTC CCCACAGCTG CCTCACTACC TCTCCTCATG 
GTCTACTTCA CTAACTTTGG CAATACAACC ATTGTGGTGC CGAAGCCCTT CCGCTGGATT 
CTTGGCCTGC ATTTGGATTT GGGCATCCTG TACTATGTCT ACATGGGACT GCTTGCAGTG 
TTCTGTACCA ATGCCATCAA CATCCTAGCG GGAATTAATG GCCTAGAGGC TGGTCAATCA 
CTAGTCATCT CTGCTTCTAT TATTGTCTTC AACCTGGTGG AGCTGGAAGG TGATTATCGG 
GACGATCATG TCTTTTCCCT CTACTTCATG ATGCCATTTT TTTTTACCAC CTTGGGATTG 
CTGTACCATA ACTGGTACCC GTCTCAGGTG TTTGTGGGAG ACACCTTCTG TTATTTTGCT 
GGCATGACCT TTGCCGTGGT GGGAATCTTG GGACACTTCA GCAAGACCAT GCTGCTCTTC 
TTTATGCCAC AAGTATTCAA TTTCCTCTAC TCACTGCCTC AGCTCTTTCA GATCATCCCC 
TGCCCTCGAC ACCGTATGCC CAGACTCAAT ACGAAGACAG GCAAACTGGA GATGAGCTAT 
TCCAAGTTCA AGACCAAGAG CCTCTCTTTC TTGGGCACGT TTATTTTAAA GGTAACAAGG 
TAG