Nkap cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nkap
Entrez Gene ID	298342
Full Name	NFKB activating protein
Synonyms	2610020o08rik
General protein information	Preferred Names NFKB activating protein Names NF-kappa-B-activating protein nuclear NF-kappaB activating protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq35

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024872.1	NP_001020043.1	NF-kappa-B-activating protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	NKAP	NP_078804.2
Pan troglodytes (chimpanzee)	NKAP	XP_003317704.1
	NKAP	XP_001084458.1
Rattus norvegicus (Norway rat)	Nkap	NP_001020043.1
Canis lupus familiaris (dog)	NKAP	XP_003640279.1
Bos taurus (cattle)	NKAP	NP_001192738.1
Mus musculus (house mouse)	Nkap	NP_080213.3

Gene Ontology
Bibliography

Related articles in PubMed

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang C, Heman-Ackah SM, Hessa T, Guha R, Martin SE, Youle RJ
Nature504(7479)291-5(2013 Dec)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Adult hematopoietic stem cells require NKAP for maintenance and survival.
Pajerowski AG, Shapiro MJ, Gwin K, Sundsbak R, Nelson-Holte M, Medina K, Shapiro VS
Blood116(15)2684-93(2010 Oct)

NKAP is a transcriptional repressor of notch signaling and is required for T cell development.
Pajerowski AG, Nguyen C, Aghajanian H, Shapiro MJ, Shapiro VS
Immunity30(5)696-707(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nkap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nkap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06724	NM_001024872.1 Latest version!	Rattus norvegicus NFKB activating protein (Nkap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nkap ( NM_001024872.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001020043.1 Rattus norvegicus NFKB activating protein (Nkap), mRNA.

Price & Availability↑

CloneID	ORa06724
Clone ID Related Accession (Same CDS sequence)	NM_001024872.1
Accession Version	NM_001024872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	NF-kappa-B-activating protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC098011.1. On Jun 17, 2005 this sequence version replaced XM_216468.3. ##Evidence-Data-START## Transcript exon combination :: BC098011.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCCTG TGTCGGGCTC GCGTAGCCCT GTACGGGAGG CCTCCGGGGG AAAGCGTCGC 
AGTTCGTCGA GGAGCCCCAA ATCCATCAAA TCTTCCCGCT CCCCGCGATG TCGCCGGTCT 
CGCTCGCGCT CTTGTTCTCG GTTCGGTGAC CGGAATGGCC TCAGCCATTC GCTGAGTGGC 
TTCAGCCAAA GCTCTCGAAA CCAATCTTAT CGCTCCCGCT CTCGATCGCG TTCTCGAGAA 
CGGCCTTCTG CACAGCGGAG TGCCCCGTTC GCGTCTTCAT CCTCGTCCGC CTATTATGGC 
GGCTACTCGC GTCCCTACGG GGGTGACAAG CCATGGCCTA GCCTGCTGGA CAAGGAAAGG 
GAGGAGAGTC TGCGGCAGAA GAGATTAAGT GAAAGAGAGA GGATTGGAGA ATTGGGAGCT 
CCTGAAGTTT GGGGACTTTC CCCAAAGAAT CCTGAACCAG ACTCTGATGA ACATACACCA 
GTAGAGGATG AAGAACCAAA GAAAAGCACT ACTTCAGCAT CTAGTTCGGA AGATGATAAG 
AAGAAGAAAA GGAAATCTAG TCGTTCAAAA GAAAGAGCCA AGAAAAAGAG AAAGAAAAAG 
TCATCTAAAA GAAAACATAA GAAGTATTCT GAAGATAGCG ACAGTGACTC AGAGTCTGAC 
ACCGACTCTA GTGATGAAGA TTCAAAAAGA AGAGCAAAGA AAGCTAAGAA AAAGGAAAAG 
AAGAAGAAAC GAAGAGGGAA GAAATACAAG AAAAAGAAGT CTAAGAAAAA CAGAAAGGAG 
TCCAGTGATT CAAGCTCTAA AGAGTCCCAA GAAGAATTTC TAGAGAATCC GTGGAAGGAT 
CGATCAAAGA CAGAAGAACC ATCAGATCTG ATTGGCCCAG AGGCTCCCAA AACACTTGCC 
TCCCAAGATG ATAAGCCCTT GAACTATGGC CACGCCCTGT TACCTGGTGA AGGTGCAGCT 
ATGGCTGAAT ATGTAAAAGC TGGCAAGCGT ATCCCGAGAA GAGGTGAAAT CGGCTTAACA 
AGTGAAGAGA TCGCATCATT CGAATGTTCC GGTTATGTAA TGAGCGGCAG CAGGCATCGC 
CGAATGGAGG CTGTCCGACT TCGGAAAGAG AACCAGATCT ACAGTGCCGA TGAGAAGAGA 
GCCCTTGCCT CCTTTAACCA AGAAGAAAGA CGGAAGAGGG AGAACAAGAT TCTGGCCAGC 
TTTCGCGAGA TGGTGTACAG AAAGACCAAA GGGAAGGATG ACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020043.1
CDS	98..1345
Translation	MAPVSGSRSPVREASGGKRRSSSRSPKSIKSSRSPRCRRSRSRSCSRFGDRNGLSHSLSGFSQSSRNQSYRSRSRSRSRERPSAQRSAPFASSSSSAYYGGYSRPYGGDKPWPSLLDKEREESLRQKRLSERERIGELGAPEVWGLSPKNPEPDSDEHTPVEDEEPKKSTTSASSSEDDKKKKRKSSRSKERAKKKRKKKSSKRKHKKYSEDSDSDSESDTDSSDEDSKRRAKKAKKKEKKKKRRGKKYKKKKSKKNRKESSDSSSKESQEEFLENPWKDRSKTEEPSDLIGPEAPKTLASQDDKPLNYGHALLPGEGAAMAEYVKAGKRIPRRGEIGLTSEEIASFECSGYVMSGSRHRRMEAVRLRKENQIYSADEKRALASFNQEERRKRENKILASFREMVYRKTKGKDDK

Target ORF information:

RefSeq Version	NM_001024872.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NFKB activating protein (Nkap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024872.1

ORF Insert Sequence:

ATGGCTCCTG TGTCGGGCTC GCGTAGCCCT GTACGGGAGG CCTCCGGGGG AAAGCGTCGC 
AGTTCGTCGA GGAGCCCCAA ATCCATCAAA TCTTCCCGCT CCCCGCGATG TCGCCGGTCT 
CGCTCGCGCT CTTGTTCTCG GTTCGGTGAC CGGAATGGCC TCAGCCATTC GCTGAGTGGC 
TTCAGCCAAA GCTCTCGAAA CCAATCTTAT CGCTCCCGCT CTCGATCGCG TTCTCGAGAA 
CGGCCTTCTG CACAGCGGAG TGCCCCGTTC GCGTCTTCAT CCTCGTCCGC CTATTATGGC 
GGCTACTCGC GTCCCTACGG GGGTGACAAG CCATGGCCTA GCCTGCTGGA CAAGGAAAGG 
GAGGAGAGTC TGCGGCAGAA GAGATTAAGT GAAAGAGAGA GGATTGGAGA ATTGGGAGCT 
CCTGAAGTTT GGGGACTTTC CCCAAAGAAT CCTGAACCAG ACTCTGATGA ACATACACCA 
GTAGAGGATG AAGAACCAAA GAAAAGCACT ACTTCAGCAT CTAGTTCGGA AGATGATAAG 
AAGAAGAAAA GGAAATCTAG TCGTTCAAAA GAAAGAGCCA AGAAAAAGAG AAAGAAAAAG 
TCATCTAAAA GAAAACATAA GAAGTATTCT GAAGATAGCG ACAGTGACTC AGAGTCTGAC 
ACCGACTCTA GTGATGAAGA TTCAAAAAGA AGAGCAAAGA AAGCTAAGAA AAAGGAAAAG 
AAGAAGAAAC GAAGAGGGAA GAAATACAAG AAAAAGAAGT CTAAGAAAAA CAGAAAGGAG 
TCCAGTGATT CAAGCTCTAA AGAGTCCCAA GAAGAATTTC TAGAGAATCC GTGGAAGGAT 
CGATCAAAGA CAGAAGAACC ATCAGATCTG ATTGGCCCAG AGGCTCCCAA AACACTTGCC 
TCCCAAGATG ATAAGCCCTT GAACTATGGC CACGCCCTGT TACCTGGTGA AGGTGCAGCT 
ATGGCTGAAT ATGTAAAAGC TGGCAAGCGT ATCCCGAGAA GAGGTGAAAT CGGCTTAACA 
AGTGAAGAGA TCGCATCATT CGAATGTTCC GGTTATGTAA TGAGCGGCAG CAGGCATCGC 
CGAATGGAGG CTGTCCGACT TCGGAAAGAG AACCAGATCT ACAGTGCCGA TGAGAAGAGA 
GCCCTTGCCT CCTTTAACCA AGAAGAAAGA CGGAAGAGGG AGAACAAGAT TCTGGCCAGC 
TTTCGCGAGA TGGTGTACAG AAAGACCAAA GGGAAGGATG ACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Adult hematopoietic stem cells require NKAP for maintenance and survival.
Blood116(15)2684-93(2010 Oct)
Pajerowski AG,Shapiro MJ,Gwin K,Sundsbak R,Nelson-Holte M,Medina K,Shapiro VS

NKAP is a transcriptional repressor of notch signaling and is required for T cell development.
Immunity30(5)696-707(2009 May)
Pajerowski AG,Nguyen C,Aghajanian H,Shapiro MJ,Shapiro VS