Timm44 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Timm44
Entrez Gene ID	29635
Full Name	translocase of inner mitochondrial membrane 44
General protein information	Preferred Names translocase of inner mitochondrial membrane 44 Names mitochondrial import inner membrane translocase subunit TIM44 translocator of inner mitochondrial membrane 44
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12p12
Summary	mouse homolog is a translocase of the inner mitochondrial membrane; can be up-regulated in hyperglycemic states [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017267.1	NP_058963.1	mitochondrial import inner membrane translocase subunit TIM44

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	TIMM44	XP_418174.2
Homo sapiens (human)	TIMM44	NP_006342.2
Canis lupus familiaris (dog)	TIMM44	XP_542123.2
Rattus norvegicus (Norway rat)	Timm44	NP_058963.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000291	XP_310830.4
Caenorhabditis elegans (roundworm)	T09B4.9	NP_491780.1
Eremothecium gossypii	AGOS_ADL118C	NP_983978.1
Bos taurus (cattle)	TIMM44	NP_001179583.1
Danio rerio (zebrafish)	LOC100537910	XP_003201001.1
Magnaporthe oryzae (rice blast fungus)	MGG_11419	XP_003713412.1
	TIMM44	XP_001098363.2
Danio rerio (zebrafish)	timm44	NP_001013525.1
Kluyveromyces lactis	KLLA0D16214g	XP_453775.1
Schizosaccharomyces pombe (fission yeast)	tim44	NP_595905.1
Xenopus tropicalis (tropical clawed frog)	timm44	NP_001120395.1
Pan troglodytes (chimpanzee)	TIMM44	XP_003316109.1
Mus musculus (house mouse)	Timm44	NP_035722.2
Drosophila melanogaster (fruit fly)	CG11779	NP_001027193.2
Saccharomyces cerevisiae (baker's yeast)	TIM44	NP_012242.1
Neurospora crassa	NCU03229	XP_965329.1

Gene Ontology
Bibliography

Related articles in PubMed

Unlocking the presequence import pathway.
Schulz C, Schendzielorz A, Rehling P
Trends in cell biology25(5)265-75(2015 May)

Impaired transport of mitochondrial transcription factor A (TFAM) and the metabolic memory phenomenon associated with the progression of diabetic retinopathy.
Santos JM, Kowluru RA
Diabetes/metabolism research and reviews29(3)204-13(2013 Mar)

Diabetic retinopathy and damage to mitochondrial structure and transport machinery.
Zhong Q, Kowluru RA
Investigative ophthalmology & visual science52(12)8739-46(2011 Nov)

Role of Magmas in protein transport and human mitochondria biogenesis.
Sinha D, Joshi N, Chittoor B, Samji P, D'Silva P
Human molecular genetics19(7)1248-62(2010 Apr)

Gene delivery of Tim44 reduces mitochondrial superoxide production and ameliorates neointimal proliferation of injured carotid artery in diabetic rats.
Matsuoka T, Wada J, Hashimoto I, Zhang Y, Eguchi J, Ogawa N, Shikata K, Kanwar YS, Makino H
Diabetes54(10)2882-90(2005 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Timm44 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Timm44 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12496	NM_017267.1 Latest version!	Rattus norvegicus translocase of inner mitochondrial membrane 44 (Timm44), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Timm44 ( NM_017267.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058963.1 Rattus norvegicus translocase of inner mitochondrial membrane 44 (Timm44), mRNA.

Price & Availability↑

CloneID	ORa12496
Clone ID Related Accession (Same CDS sequence)	NM_017267.1
Accession Version	NM_017267.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Rattus norvegicus(Norway rat)
Product	mitochondrial import inner membrane translocase subunit TIM44
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB006452.1. ##Evidence-Data-START## Transcript exon combination :: AB006452.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGCCG CAGCTCTGCG GGGCGGCTGG TGCCGCTGTC CGCGGAGGTG CCTAGGCAGC 
GGAATACAAT TTCTCTCCAG TCACAACCTA CCCCGTGGTG GTTCAAGCTA CCAGATAAGC 
CGGCCAGGAG GAGAGCTGAC GCTGACTAAA TCTTATTCTT CTGGGAGCAG AAAAGGGTTT 
TTGTCAGGCT TGCTAGATAA CATCAAACAA GAATTAGCCC AAAACAAAGA GATGAAAGAA 
AGTATAAAGA AGTTCCGAGA TGAGGCCAAG AAACTAGAAG AGTCGGATGC CCTTCAGGAA 
GCCAGAAGAA AATATAAAAC CATCGAATCA GAAACTGTGC GAACTAGTGA AGCAATAAAG 
AAGAAGCTCG GAGAATTGAC AGGCACAGTG AAGGAGAGTC TTGATGAAGT CAGTAAGAGT 
GACCTTGGCC GCAAGATCAA GGAAGGAGTA GAAGAGGCTG CCAGGACAGC CAAGCAGTCA 
GCAGAGTCAG TGTCCAAGGG CGGGGAGAAG CTGGGCAAGA CGGCGGCCTT CAAAGCCATC 
TCCCAGGGTG TAGAGTCGGT GAAGAAGGAG ATTGATGAGA GCGTGCTCGG ACACACAGGG 
ACATACCGCC GGCCTGAGCG GCTCCGGAAA AGGACAGAGT TTGCTGGAGC AAAGTTCAAA 
GAAAGCAAAG TGTTCGAGGC TAATGAGGAA GCATTAGGGG TTGTGCTACA CAAGGACTCC 
AAGTGGTACC AGCAGTGGAA GGACTTCAAA GATAACAATG TTGTATTCAA CCGCTTCTTT 
GAGATGAAGA TGAAGTATGA TGAAAGTGAC AATGTTCTTA TCCGGGCATC CCGGGCCTTA 
ACCGACAAGG TCACTGACTT GCTGGGAGGT CTTTTCTCAA AGACAGAAAT GTCAGAGGTG 
CTCACGGAGA TCCTGAGAGT GGACCCAACC TTTGACAAGG ACCGCTTTCT TCATCAGTGT 
GAGACTGACA TTATCCCCAA CATCCTAGAG GCCATGATTT CCGGGGAACT TGACATTCTC 
AAAGACTGGT GCTATGAAGC TACCTACAAC CAACTGGCCC ACTCTATCCA GCAGGCCAAG 
GCTCTGGGCC TCCAGTTCCA CTCCCGGATC CTGGATATCA GCAATGTGGA CCTGGCCATG 
GGCAAGATGA TGGAGCAGGG CCCCGTGCTG ATTGTTACCT TCCAGGCCCA GCTGGTGATG 
GTGATCAAGA ACCCCAAAGG CGAGGTGTTT GACGGTGACC CGGACAAGGT GCTGCGAATG 
CTGTATGTGT GGGCACTCTG CAGAGACCAG GAGGAGCTCA ACCCTTACGC TGCCTGGCGC 
CTTCTGGACA TATCAGCCTC TAGCACAGAG CAGATCCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058963.1
CDS	24..1385
Translation	MAAAALRGGWCRCPRRCLGSGIQFLSSHNLPRGGSSYQISRPGGELTLTKSYSSGSRKGFLSGLLDNIKQELAQNKEMKESIKKFRDEAKKLEESDALQEARRKYKTIESETVRTSEAIKKKLGELTGTVKESLDEVSKSDLGRKIKEGVEEAARTAKQSAESVSKGGEKLGKTAAFKAISQGVESVKKEIDESVLGHTGTYRRPERLRKRTEFAGAKFKESKVFEANEEALGVVLHKDSKWYQQWKDFKDNNVVFNRFFEMKMKYDESDNVLIRASRALTDKVTDLLGGLFSKTEMSEVLTEILRVDPTFDKDRFLHQCETDIIPNILEAMISGELDILKDWCYEATYNQLAHSIQQAKALGLQFHSRILDISNVDLAMGKMMEQGPVLIVTFQAQLVMVIKNPKGEVFDGDPDKVLRMLYVWALCRDQEELNPYAAWRLLDISASSTEQIL

Target ORF information:

RefSeq Version	NM_017267.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus translocase of inner mitochondrial membrane 44 (Timm44), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017267.1

ORF Insert Sequence:

ATGGCGGCCG CAGCTCTGCG GGGCGGCTGG TGCCGCTGTC CGCGGAGGTG CCTAGGCAGC 
GGAATACAAT TTCTCTCCAG TCACAACCTA CCCCGTGGTG GTTCAAGCTA CCAGATAAGC 
CGGCCAGGAG GAGAGCTGAC GCTGACTAAA TCTTATTCTT CTGGGAGCAG AAAAGGGTTT 
TTGTCAGGCT TGCTAGATAA CATCAAACAA GAATTAGCCC AAAACAAAGA GATGAAAGAA 
AGTATAAAGA AGTTCCGAGA TGAGGCCAAG AAACTAGAAG AGTCGGATGC CCTTCAGGAA 
GCCAGAAGAA AATATAAAAC CATCGAATCA GAAACTGTGC GAACTAGTGA AGCAATAAAG 
AAGAAGCTCG GAGAATTGAC AGGCACAGTG AAGGAGAGTC TTGATGAAGT CAGTAAGAGT 
GACCTTGGCC GCAAGATCAA GGAAGGAGTA GAAGAGGCTG CCAGGACAGC CAAGCAGTCA 
GCAGAGTCAG TGTCCAAGGG CGGGGAGAAG CTGGGCAAGA CGGCGGCCTT CAAAGCCATC 
TCCCAGGGTG TAGAGTCGGT GAAGAAGGAG ATTGATGAGA GCGTGCTCGG ACACACAGGG 
ACATACCGCC GGCCTGAGCG GCTCCGGAAA AGGACAGAGT TTGCTGGAGC AAAGTTCAAA 
GAAAGCAAAG TGTTCGAGGC TAATGAGGAA GCATTAGGGG TTGTGCTACA CAAGGACTCC 
AAGTGGTACC AGCAGTGGAA GGACTTCAAA GATAACAATG TTGTATTCAA CCGCTTCTTT 
GAGATGAAGA TGAAGTATGA TGAAAGTGAC AATGTTCTTA TCCGGGCATC CCGGGCCTTA 
ACCGACAAGG TCACTGACTT GCTGGGAGGT CTTTTCTCAA AGACAGAAAT GTCAGAGGTG 
CTCACGGAGA TCCTGAGAGT GGACCCAACC TTTGACAAGG ACCGCTTTCT TCATCAGTGT 
GAGACTGACA TTATCCCCAA CATCCTAGAG GCCATGATTT CCGGGGAACT TGACATTCTC 
AAAGACTGGT GCTATGAAGC TACCTACAAC CAACTGGCCC ACTCTATCCA GCAGGCCAAG 
GCTCTGGGCC TCCAGTTCCA CTCCCGGATC CTGGATATCA GCAATGTGGA CCTGGCCATG 
GGCAAGATGA TGGAGCAGGG CCCCGTGCTG ATTGTTACCT TCCAGGCCCA GCTGGTGATG 
GTGATCAAGA ACCCCAAAGG CGAGGTGTTT GACGGTGACC CGGACAAGGT GCTGCGAATG 
CTGTATGTGT GGGCACTCTG CAGAGACCAG GAGGAGCTCA ACCCTTACGC TGCCTGGCGC 
CTTCTGGACA TATCAGCCTC TAGCACAGAG CAGATCCTTT GA