Mecr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mecr
Entrez Gene ID	29470
Full Name	mitochondrial trans-2-enoyl-CoA reductase
Synonyms	Nrbf1
General protein information	Preferred Names mitochondrial trans-2-enoyl-CoA reductase Names enoyl-[acyl-carrier-protein] reductase, mitochondrial trans-2-enoyl-CoA reductase, mitochondrial 2-enoyl thioester reductase NRBF-1 nuclear receptor-binding factor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	interacts with PPARalpha and with various nuclear hormone receptors [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006239009.3	XP_006239071.1	trans-2-enoyl-CoA reductase, mitochondrial isoform X1
NM_017209.1	NP_058905.1	enoyl-[acyl-carrier-protein] reductase, mitochondrial precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mecr	NP_079573.2
Danio rerio (zebrafish)	mecr	NP_001017852.1
Kluyveromyces lactis	KLLA0F24552g	XP_456173.1
Schizosaccharomyces pombe (fission yeast)	etr1	NP_594891.1
Homo sapiens (human)	MECR	NP_057095.3
Canis lupus familiaris (dog)	MECR	XP_535334.2
Magnaporthe oryzae (rice blast fungus)	MGG_02566	XP_003721237.1
Xenopus tropicalis (tropical clawed frog)	mecr	NP_001016371.1
Rattus norvegicus (Norway rat)	Mecr	NP_058905.1
Gallus gallus (chicken)	MECR	XP_417748.4
Drosophila melanogaster (fruit fly)	CG16935	NP_610914.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011834	XP_320682.4
Eremothecium gossypii	AGOS_AEL081W	NP_984780.2
Pan troglodytes (chimpanzee)	MECR	XP_001156115.1
Oryza sativa (rice)	Os12g0102100	NP_001065233.2
	MECR	XP_001113772.1
Bos taurus (cattle)	MECR	NP_858055.1
Caenorhabditis elegans (roundworm)	mecr-1	NP_496800.1
Saccharomyces cerevisiae (baker's yeast)	ETR1	NP_009582.1
Neurospora crassa	NCU00655	XP_965795.1
Arabidopsis thaliana (thale cress)	AT3G45770	NP_566881.1
Oryza sativa (rice)	Os11g0102500	NP_001176296.1

Gene Ontology
Bibliography

Related articles in PubMed

MECR Mutations Cause Childhood-Onset Dystonia and Optic Atrophy, a Mitochondrial Fatty Acid Synthesis Disorder.
Heimer G, Ker?t?r JM, Riley LG, Balasubramaniam S, Eyal E, Pietik?inen LP, Hiltunen JK, Marek-Yagel D, Hamada J, Gregory A, Rogers C, Hogarth P, Nance MA, Shalva N, Veber A, Tzadok M, Nissenkorn A, Tonduti D, Renaldo F, , Kraoua I, Panteghini C, Valletta L, Garavaglia B, Cowley MJ, Gayevskiy V, Roscioli T, Silberstein JM, Hoffmann C, Raas-Rothschild A, Tiranti V, Anikster Y, Christodoulou J, Kastaniotis AJ, Ben-Zeev B, Hayflick SJ
American journal of human genetics99(6)1229-1244(2016 Dec)

Characterization of 2-enoyl thioester reductase from mammals. An ortholog of YBR026p/MRF1'p of the yeast mitochondrial fatty acid synthesis type II.
Miinalainen IJ, Chen ZJ, Torkko JM, Piril? PL, Sormunen RT, Bergmann U, Qin YM, Hiltunen JK
The Journal of biological chemistry278(22)20154-61(2003 May)

Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors.
Masuda N, Yasumo H, Furusawa T, Tsukamoto T, Sadano H, Osumi T
Gene221(2)225-33(1998 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Mecr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mecr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12467	NM_017209.1 Latest version!	Rattus norvegicus mitochondrial trans-2-enoyl-CoA reductase (Mecr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa28681	XM_006239009.3 Latest version!	Rattus norvegicus mitochondrial trans-2-enoyl-CoA reductase (Mecr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mecr ( NM_017209.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058905.1 Rattus norvegicus mitochondrial trans-2-enoyl-CoA reductase (Mecr), mRNA.

Price & Availability↑

CloneID	ORa12467
Clone ID Related Accession (Same CDS sequence)	NM_017209.1
Accession Version	NM_017209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	enoyl-[acyl-carrier-protein] reductase, mitochondrial precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB015724.1. ##Evidence-Data-START## Transcript exon combination :: AB015724.1, FQ210707.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTGGTCA GCCGGCGACT GACAGGGGCG CGAGCCCGGG CACCTCTGCT TGCGAGTCTC 
CTCGAGGCTT GGTGCCGCCA GGGCAGGACC ACCTCCTCCT ACTCCGCTTT CTCGGAGCCG 
TCGCATGTGC GGGCGCTGGT CTATGGCAAC CACGGGGATC CAGCCAAGGT CATCCAACTG 
AAGAACCTGG AGCTCACTGC TGTGGAAGGA TCTGACGTCC ATGTGAAGAT GCTGGCAGCC 
CCTATCAATC CATCTGACAT AAATATGATC CAAGGGAACT ATGGCCTCCT TCCCAAGCTG 
CCTGCTGTTG GAGGGAATGA AGGTGTTGGA CAGGTGATAG CAGTGGGCAG CAGTGTGTCT 
GGATTGAAGC CAGGAGATTG GGTGATCCCA GCAAATGCTG GTTTGGGAAC CTGGCGGACT 
GAGGCGGTGT TCAGTGAGGA AGCGCTGATT GGAGTCCCTA AGGACATCCC TCTCCAGAGT 
GCTGCCACCC TAGGTGTCAA CCCCTGCACA GCCTACAGGA TGTTGGTGGA CTTTGAACAG 
CTACAACCAG GGGACTCTGT CATCCAGAAT GCGTCCAACA GTGGAGTAGG GCAAGCAGTC 
ATTCAGATCG CCTCAGCCCT TGGCCTAAAG ACCATCAACG TGATCCGAGA CAGACCTGAC 
ATCAAGAAGC TAACTGACAG ACTGAAGGAT CTAGGAGCTG ATTATGTCCT CACAGAGGAG 
GAGCTAAGGA TGCCCGAGAC CAAAAACATC TTCAAGGACC TGCCGCTGCC CCGACTGGCT 
CTCAACTGTG TCGGTGGGAA GAGTTCCACA GAGCTGCTCC GGCACCTAGC GCCCGGAGGA 
ACCATGGTGA CCTATGGAGG AATGGCCAAG CAGCCTGTAA CAGCCTCTGT GAGTATGCTC 
ATTTTTAAGG ACCTCAAACT TCGTGGCTTT TGGTTGTCCC AGTGGAAGAA GAACCATAGT 
CCAGATGAGT TCAAGGAGCT GATTCTCATT CTCTGCAACC TCATCCGCCA AGGCCAGCTC 
ACAGCCCCTG CCTGGTCCGG GATTCCACTG CAGGACTACC AGCAGGCTTT GGAAGCCTCC 
ATGAAGCCTT TTGTGTCTTT GAAGCAGATT CTCACTATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058905.1
CDS	20..1141
Translation	MLVSRRLTGARARAPLLASLLEAWCRQGRTTSSYSAFSEPSHVRALVYGNHGDPAKVIQLKNLELTAVEGSDVHVKMLAAPINPSDINMIQGNYGLLPKLPAVGGNEGVGQVIAVGSSVSGLKPGDWVIPANAGLGTWRTEAVFSEEALIGVPKDIPLQSAATLGVNPCTAYRMLVDFEQLQPGDSVIQNASNSGVGQAVIQIASALGLKTINVIRDRPDIKKLTDRLKDLGADYVLTEEELRMPETKNIFKDLPLPRLALNCVGGKSSTELLRHLAPGGTMVTYGGMAKQPVTASVSMLIFKDLKLRGFWLSQWKKNHSPDEFKELILILCNLIRQGQLTAPAWSGIPLQDYQQALEASMKPFVSLKQILTM

Target ORF information:

RefSeq Version	NM_017209.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitochondrial trans-2-enoyl-CoA reductase (Mecr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017209.1

ORF Insert Sequence:

ATGTTGGTCA GCCGGCGACT GACAGGGGCG CGAGCCCGGG CACCTCTGCT TGCGAGTCTC 
CTCGAGGCTT GGTGCCGCCA GGGCAGGACC ACCTCCTCCT ACTCCGCTTT CTCGGAGCCG 
TCGCATGTGC GGGCGCTGGT CTATGGCAAC CACGGGGATC CAGCCAAGGT CATCCAACTG 
AAGAACCTGG AGCTCACTGC TGTGGAAGGA TCTGACGTCC ATGTGAAGAT GCTGGCAGCC 
CCTATCAATC CATCTGACAT AAATATGATC CAAGGGAACT ATGGCCTCCT TCCCAAGCTG 
CCTGCTGTTG GAGGGAATGA AGGTGTTGGA CAGGTGATAG CAGTGGGCAG CAGTGTGTCT 
GGATTGAAGC CAGGAGATTG GGTGATCCCA GCAAATGCTG GTTTGGGAAC CTGGCGGACT 
GAGGCGGTGT TCAGTGAGGA AGCGCTGATT GGAGTCCCTA AGGACATCCC TCTCCAGAGT 
GCTGCCACCC TAGGTGTCAA CCCCTGCACA GCCTACAGGA TGTTGGTGGA CTTTGAACAG 
CTACAACCAG GGGACTCTGT CATCCAGAAT GCGTCCAACA GTGGAGTAGG GCAAGCAGTC 
ATTCAGATCG CCTCAGCCCT TGGCCTAAAG ACCATCAACG TGATCCGAGA CAGACCTGAC 
ATCAAGAAGC TAACTGACAG ACTGAAGGAT CTAGGAGCTG ATTATGTCCT CACAGAGGAG 
GAGCTAAGGA TGCCCGAGAC CAAAAACATC TTCAAGGACC TGCCGCTGCC CCGACTGGCT 
CTCAACTGTG TCGGTGGGAA GAGTTCCACA GAGCTGCTCC GGCACCTAGC GCCCGGAGGA 
ACCATGGTGA CCTATGGAGG AATGGCCAAG CAGCCTGTAA CAGCCTCTGT GAGTATGCTC 
ATTTTTAAGG ACCTCAAACT TCGTGGCTTT TGGTTGTCCC AGTGGAAGAA GAACCATAGT 
CCAGATGAGT TCAAGGAGCT GATTCTCATT CTCTGCAACC TCATCCGCCA AGGCCAGCTC 
ACAGCCCCTG CCTGGTCCGG GATTCCACTG CAGGACTACC AGCAGGCTTT GGAAGCCTCC 
ATGAAGCCTT TTGTGTCTTT GAAGCAGATT CTCACTATGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mecr ( XM_006239009.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006239071.1 Rattus norvegicus mitochondrial trans-2-enoyl-CoA reductase (Mecr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa28681
Clone ID Related Accession (Same CDS sequence)	XM_006239009.3
Accession Version	XM_006239009.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	trans-2-enoyl-CoA reductase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006239009.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGCAG CCCCTATCAA TCCATCTGAC ATAAATATGA TCCAAGGGAA CTATGGCCTC 
CTTCCCAAGC TGCCTGCTGT TGGAGGGAAT GAAGGTGTTG GACAGGTGAT AGCAGTGGGC 
AGCAGTGTGT CTGGATTGAA GCCAGGAGAT TGGGTGATCC CAGCAAATGC TGGTTTGGGA 
ACCTGGCGGA CTGAGGCGGT GTTCAGTGAG GAAGCGCTGA TTGGAGTCCC TAAGGACATC 
CCTCTCCAGA GTGCTGCCAC CCTAGGTGTC AACCCCTGCA CAGCCTACAG GATGTTGGTG 
GACTTTGAAC AGCTACAACC AGGGGACTCT GTCATCCAGA ATGCGTCCAA CAGTGGAGTA 
GGGCAAGCAG TCATTCAGAT CGCCTCAGCC CTTGGCCTAA AGACCATCAA CGTGATCCGA 
GACAGACCTG ACATCAAGAA GCTAACTGAC AGACTGAAGG ATCTAGGAGC TGATTATGTC 
CTCACAGAGG AGGAGCTAAG GATGCCCGAG ACCAAAAACA TCTTCAAGGA CCTGCCGCTG 
CCCCGACTGG CTCTCAACTG TGTCGGTGGG AAGAGTTCCA CAGAGCTGCT CCGGCACCTA 
GCGCCCGGAG GAACCATGGT GACCTATGGA GGAATGGCCA AGCAGCCTGT AACAGCCTCT 
GTGAGTATGC TCATTTTTAA GGACCTCAAA CTTCGTGGCT TTTGGTTGTC CCAGTGGAAG 
AAGAACCATA GTCCAGATGA GTTCAAGGAG CTGATTCTCT TTCTCTGCAA CCTCATCCGC 
CAAGGCCAGC TCACAGCCCC TGCCTGGTCC GGGATTCCAC TGCAGGACTA CCAGCAGGCT 
TTGGAAGCCT CCATGAAGCC TTTTGTGTCT TCGAAGCAGA TTCTCACTAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006239071.1
CDS	349..1242
Translation	MLAAPINPSDINMIQGNYGLLPKLPAVGGNEGVGQVIAVGSSVSGLKPGDWVIPANAGLGTWRTEAVFSEEALIGVPKDIPLQSAATLGVNPCTAYRMLVDFEQLQPGDSVIQNASNSGVGQAVIQIASALGLKTINVIRDRPDIKKLTDRLKDLGADYVLTEEELRMPETKNIFKDLPLPRLALNCVGGKSSTELLRHLAPGGTMVTYGGMAKQPVTASVSMLIFKDLKLRGFWLSQWKKNHSPDEFKELILFLCNLIRQGQLTAPAWSGIPLQDYQQALEASMKPFVSSKQILTM

Target ORF information:

RefSeq Version	XM_006239009.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mitochondrial trans-2-enoyl-CoA reductase (Mecr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006239009.3

ORF Insert Sequence:

ATGCTGGCAG CCCCTATCAA TCCATCTGAC ATAAATATGA TCCAAGGGAA CTATGGCCTC 
CTTCCCAAGC TGCCTGCTGT TGGAGGGAAT GAAGGTGTTG GACAGGTGAT AGCAGTGGGC 
AGCAGTGTGT CTGGATTGAA GCCAGGAGAT TGGGTGATCC CAGCAAATGC TGGTTTGGGA 
ACCTGGCGGA CTGAGGCGGT GTTCAGTGAG GAAGCGCTGA TTGGAGTCCC TAAGGACATC 
CCTCTCCAGA GTGCTGCCAC CCTAGGTGTC AACCCCTGCA CAGCCTACAG GATGTTGGTG 
GACTTTGAAC AGCTACAACC AGGGGACTCT GTCATCCAGA ATGCGTCCAA CAGTGGAGTA 
GGGCAAGCAG TCATTCAGAT CGCCTCAGCC CTTGGCCTAA AGACCATCAA CGTGATCCGA 
GACAGACCTG ACATCAAGAA GCTAACTGAC AGACTGAAGG ATCTAGGAGC TGATTATGTC 
CTCACAGAGG AGGAGCTAAG GATGCCCGAG ACCAAAAACA TCTTCAAGGA CCTGCCGCTG 
CCCCGACTGG CTCTCAACTG TGTCGGTGGG AAGAGTTCCA CAGAGCTGCT CCGGCACCTA 
GCGCCCGGAG GAACCATGGT GACCTATGGA GGAATGGCCA AGCAGCCTGT AACAGCCTCT 
GTGAGTATGC TCATTTTTAA GGACCTCAAA CTTCGTGGCT TTTGGTTGTC CCAGTGGAAG 
AAGAACCATA GTCCAGATGA GTTCAAGGAG CTGATTCTCT TTCTCTGCAA CCTCATCCGC 
CAAGGCCAGC TCACAGCCCC TGCCTGGTCC GGGATTCCAC TGCAGGACTA CCAGCAGGCT 
TTGGAAGCCT CCATGAAGCC TTTTGTGTCT TCGAAGCAGA TTCTCACTAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MECR Mutations Cause Childhood-Onset Dystonia and Optic Atrophy, a Mitochondrial Fatty Acid Synthesis Disorder.
American journal of human genetics99(6)1229-1244(2016 Dec)
Heimer G,Ker?t?r JM,Riley LG,Balasubramaniam S,Eyal E,Pietik?inen LP,Hiltunen JK,Marek-Yagel D,Hamada J,Gregory A,Rogers C,Hogarth P,Nance MA,Shalva N,Veber A,Tzadok M,Nissenkorn A,Tonduti D,Renaldo F,,Kraoua I,Panteghini C,Valletta L,Garavaglia B,Cowley MJ,Gayevskiy V,Roscioli T,Silberstein JM,Hoffmann C,Raas-Rothschild A,Tiranti V,Anikster Y,Christodoulou J,Kastaniotis AJ,Ben-Zeev B,Hayflick SJ

Characterization of 2-enoyl thioester reductase from mammals. An ortholog of YBR026p/MRF1'p of the yeast mitochondrial fatty acid synthesis type II.
The Journal of biological chemistry278(22)20154-61(2003 May)
Miinalainen IJ,Chen ZJ,Torkko JM,Piril? PL,Sormunen RT,Bergmann U,Qin YM,Hiltunen JK

Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors.
Gene221(2)225-33(1998 Oct)
Masuda N,Yasumo H,Furusawa T,Tsukamoto T,Sadano H,Osumi T