Mcpt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mcpt2
Entrez Gene ID	29266
Full Name	mast cell protease 2
General protein information	Preferred Names mast cell protease 2 Names mast cell protease 2 group-specific protease mast cell peptidase 2 mast cell protease II rMCP-2 rMCP-II
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p12
Summary	member of a family of mast cell serine proteases [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_172044.1	NP_742041.1	mast cell protease 2 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mcpt1	NP_032596.1
Rattus norvegicus (Norway rat)	Mcpt1l3	XP_006252116.1
Rattus norvegicus (Norway rat)	Mcpt1	NP_058841.1
Rattus norvegicus (Norway rat)	Mcpt2	NP_742041.1

Gene Ontology
Bibliography

Related articles in PubMed

Development and characterization of an effective food allergy model in Brown Norway rats.
Abril-Gil M, Garcia-Just A, P?rez-Cano FJ, Franch ?, Castell M
PloS one10(4)e0125314(2015)

rMCP-2, the Major Rat Mucosal Mast Cell Protease, an Analysis of Its Extended Cleavage Specificity and Its Potential Role in Regulating Intestinal Permeability by the Cleavage of Cell Adhesion and Junction Proteins.
Fu Z, Thorpe M, Hellman L
PloS one10(6)e0131720(2015)

Expulsion of secondary Trichinella spiralis infection in rats occurs independently of mucosal mast cell release of mast cell protease II.
Blum LK, Thrasher SM, Gagliardo LF, Fabre V, Appleton JA
Journal of immunology (Baltimore, Md. : 1950)183(9)5816-22(2009 Nov)

The structure of rat mast cell protease II at 1.9-A resolution.
Remington SJ, Woodbury RG, Reynolds RA, Matthews BW, Neurath H
Biochemistry27(21)8097-105(1988 Oct)

Cloning of the mast cell protease, RMCP II. Evidence for cell-specific expression and a multi-gene family.
Benfey PN, Yin FH, Leder P
The Journal of biological chemistry262(11)5377-84(1987 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results show both an effective and relatively rapid model of FA assessed by means of specific antibody titres and the high production of RMCP-II and its intestinal gene expression
Title: Development and characterization of an effective food allergy model in Brown Norway rats.

Phage display analysis showed that rMCP-2 is a chymase with a specificity similar to the corresponding enzyme in mice, mMCP-1, with a preference for Phe or Tyr in the P1 position, and a general preference for aliphatic amino acids both upstream and downstream of the cleavage site.
Title: rMCP-2, the Major Rat Mucosal Mast Cell Protease, an Analysis of Its Extended Cleavage Specificity and Its Potential Role in Regulating Intestinal Permeability by the Cleavage of Cell Adhesion and Junction Proteins.

Rapid expulsion of Trichinella spiralis first-stage larvae from rat intestine can occur in the absence of either intestinal mastocytosis or mast cell protease II release.
Title: Expulsion of secondary Trichinella spiralis infection in rats occurs independently of mucosal mast cell release of mast cell protease II.

The following Mcpt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mcpt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05910	NM_172044.1 Latest version!	Rattus norvegicus mast cell protease 2 (Mcpt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mcpt2 ( NM_172044.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_742041.1 Rattus norvegicus mast cell protease 2 (Mcpt2), mRNA.

Price & Availability↑

CloneID	ORa05910
Clone ID Related Accession (Same CDS sequence)	NM_172044.1
Accession Version	NM_172044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	mast cell protease 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J02712.1. ##Evidence-Data-START## Transcript exon combination :: CF110755.1, FM059853.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGCAGGCCC TACTATTCCT GATGGCTCTT CTCTTGCCTT CTGGAGCTGG AGCTGAGGAA 
ATTATCGGTG GTGTGGAGTC TATTCCTCAC TCCCGCCCTT ACATGGCCCA TCTGGACATC 
GTCACTGAGA AAGGTTTAAG GGTCATCTGT GGTGGGTTTC TCATAAGCCG TCAATTTGTG 
CTGACTGCTG CACATTGTAA AGGAAGAGAA ATCACAGTCA TCCTTGGAGC CCATGATGTG 
AGAAAGCGAG AATCCACACA GCAGAAGATA AAAGTCGAAA AACAAATCAT TCACGAAAGT 
TACAACTCCG TTCCCAATCT TCATGACATC ATGTTACTGA AGCTTGAAAA AAAAGTTGAG 
TTGACTCCTG CTGTGAATGT AGTTCCTCTG CCCAGTCCCT CTGACTTTAT CCACCCTGGG 
GCAATGTGTT GGGCAGCTGG ATGGGGGAAA ACTGGAGTTA GAGATCCTAC CTCGTATACA 
CTGAGAGAGG TTGAACTGAG AATCATGGAT GAAAAGGCCT GTGTGGACTA CAGGTATTAT 
GAATACAAAT TCCAGGTCTG CGTGGGCAGT CCCACAACTT TAAGAGCAGC ATTCATGGGA 
GACTCTGGCG GACCGCTACT GTGTGCTGGT GTGGCTCATG GTATTGTATC TTATGGGCAT 
CCAGATGCAA AGCCCCCTGC AATCTTCACC CGAGTCTCCA CATATGTGCC CTGGATTAAT 
GCAGTCATTA ATACAAGTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_742041.1
CDS	1..744
Translation	MQALLFLMALLLPSGAGAEEIIGGVESIPHSRPYMAHLDIVTEKGLRVICGGFLISRQFVLTAAHCKGREITVILGAHDVRKRESTQQKIKVEKQIIHESYNSVPNLHDIMLLKLEKKVELTPAVNVVPLPSPSDFIHPGAMCWAAGWGKTGVRDPTSYTLREVELRIMDEKACVDYRYYEYKFQVCVGSPTTLRAAFMGDSGGPLLCAGVAHGIVSYGHPDAKPPAIFTRVSTYVPWINAVINTSS

Target ORF information:

RefSeq Version	NM_172044.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus mast cell protease 2 (Mcpt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_172044.1

ORF Insert Sequence:

ATGCAGGCCC TACTATTCCT GATGGCTCTT CTCTTGCCTT CTGGAGCTGG AGCTGAGGAA 
ATTATCGGTG GTGTGGAGTC TATTCCTCAC TCCCGCCCTT ACATGGCCCA TCTGGACATC 
GTCACTGAGA AAGGTTTAAG GGTCATCTGT GGTGGGTTTC TCATAAGCCG TCAATTTGTG 
CTGACTGCTG CACATTGTAA AGGAAGAGAA ATCACAGTCA TCCTTGGAGC CCATGATGTG 
AGAAAGCGAG AATCCACACA GCAGAAGATA AAAGTCGAAA AACAAATCAT TCACGAAAGT 
TACAACTCCG TTCCCAATCT TCATGACATC ATGTTACTGA AGCTTGAAAA AAAAGTTGAG 
TTGACTCCTG CTGTGAATGT AGTTCCTCTG CCCAGTCCCT CTGACTTTAT CCACCCTGGG 
GCAATGTGTT GGGCAGCTGG ATGGGGGAAA ACTGGAGTTA GAGATCCTAC CTCGTATACA 
CTGAGAGAGG TTGAACTGAG AATCATGGAT GAAAAGGCCT GTGTGGACTA CAGGTATTAT 
GAATACAAAT TCCAGGTCTG CGTGGGCAGT CCCACAACTT TAAGAGCAGC ATTCATGGGA 
GACTCTGGCG GACCGCTACT GTGTGCTGGT GTGGCTCATG GTATTGTATC TTATGGGCAT 
CCAGATGCAA AGCCCCCTGC AATCTTCACC CGAGTCTCCA CATATGTGCC CTGGATTAAT 
GCAGTCATTA ATACAAGTAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Development and characterization of an effective food allergy model in Brown Norway rats.
PloS one10(4)e0125314(2015)
Abril-Gil M,Garcia-Just A,P?rez-Cano FJ,Franch ?,Castell M

rMCP-2, the Major Rat Mucosal Mast Cell Protease, an Analysis of Its Extended Cleavage Specificity and Its Potential Role in Regulating Intestinal Permeability by the Cleavage of Cell Adhesion and Junction Proteins.
PloS one10(6)e0131720(2015)
Fu Z,Thorpe M,Hellman L

Expulsion of secondary Trichinella spiralis infection in rats occurs independently of mucosal mast cell release of mast cell protease II.
Journal of immunology (Baltimore, Md. : 1950)183(9)5816-22(2009 Nov)
Blum LK,Thrasher SM,Gagliardo LF,Fabre V,Appleton JA

The structure of rat mast cell protease II at 1.9-A resolution.
Biochemistry27(21)8097-105(1988 Oct)
Remington SJ,Woodbury RG,Reynolds RA,Matthews BW,Neurath H

Cloning of the mast cell protease, RMCP II. Evidence for cell-specific expression and a multi-gene family.
The Journal of biological chemistry262(11)5377-84(1987 Apr)
Benfey PN,Yin FH,Leder P