Ube2k cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ube2k
Entrez Gene ID	289623
Full Name	ubiquitin-conjugating enzyme E2K
Synonyms	E2-25k,Hip2,Hypg,Lig
General protein information	Preferred Names ubiquitin-conjugating enzyme E2K Names ubiquitin-conjugating enzyme E2 K huntingtin interacting protein 2 ubiquitin-conjugating enzyme E2-25K ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14p11

mRNA and Protein(s)

mRNA	Protein	Name
XM_017599107.1	XP_017454596.1	ubiquitin-conjugating enzyme E2 K isoform X1
NM_001106006.1	NP_001099476.1	ubiquitin-conjugating enzyme E2 K

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Ube2k	NP_001099476.1
Oryza sativa (rice)	Os01g0925800	NP_001045259.1
Xenopus tropicalis (tropical clawed frog)	ube2k	NP_001005662.1
Danio rerio (zebrafish)	ube2ka	NP_001013500.1
Drosophila melanogaster (fruit fly)	UbcD4	NP_524010.2
Caenorhabditis elegans (roundworm)	ubc-21	NP_509502.3
Saccharomyces cerevisiae (baker's yeast)	UBC1	NP_010462.3
Arabidopsis thaliana (thale cress)	UBC27	NP_001190508.1
Pan troglodytes (chimpanzee)	UBE2K	NP_001233454.1
Canis lupus familiaris (dog)	UBE2K	XP_003434442.1
Mus musculus (house mouse)	Ube2k	NP_058066.2
Kluyveromyces lactis	KLLA0C18656g	XP_453031.1
Magnaporthe oryzae (rice blast fungus)	MGG_05478	XP_003710343.1
Homo sapiens (human)	UBE2K	NP_005330.1
	UBE2K	XP_001093369.1
Bos taurus (cattle)	UBE2K	NP_776505.1
Gallus gallus (chicken)	UBE2K	NP_001026711.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004889	XP_314290.2
Danio rerio (zebrafish)	ube2kb	NP_001008611.1
Caenorhabditis elegans (roundworm)	ubc-20	NP_497174.1
Eremothecium gossypii	AGOS_ADR169C	NP_984266.1
Schizosaccharomyces pombe (fission yeast)	ubc1	NP_596239.1
Neurospora crassa	NCU01225	XP_961591.1

Gene Ontology
Bibliography

Related articles in PubMed

Unanchored K48-linked polyubiquitin synthesized by the E3-ubiquitin ligase TRIM6 stimulates the interferon-IKK? kinase-mediated antiviral response.
Rajsbaum R, Versteeg GA, Schmid S, Maestre AM, Belicha-Villanueva A, Mart?nez-Romero C, Patel JR, Morrison J, Pisanelli G, Miorin L, Laurent-Rolle M, Moulton HM, Stein DA, Fernandez-Sesma A, tenOever BR, Garc?a-Sastre A
Immunity40(6)880-95(2014 Jun)

The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
David Y, Ziv T, Admon A, Navon A
The Journal of biological chemistry285(12)8595-604(2010 Mar)

Expression of TMEM16 paralogs during murine embryogenesis.
Rock JR, Harfe BD
Developmental dynamics : an official publication of the American Association of Anatomists237(9)2566-74(2008 Sep)

E2-25K/Hip-2 regulates caspase-12 in ER stress-mediated Abeta neurotoxicity.
Song S, Lee H, Kam TI, Tai ML, Lee JY, Noh JY, Shim SM, Seo SJ, Kong YY, Nakagawa T, Chung CW, Choi DY, Oubrahim H, Jung YK
The Journal of cell biology182(4)675-84(2008 Aug)

Characterization of a novel ubiquitin-conjugating enzyme that regulates beta1,4-galactosyltransferase-1 in embryonic stem cells.
Wassler MJ, Shur BD, Zhou W, Geng YJ
Stem cells (Dayton, Ohio)26(8)2006-18(2008 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Ube2k gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ube2k cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11819	NM_001106006.1 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2K (Ube2k), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa58246	XM_017599107.1 Latest version!	Rattus norvegicus ubiquitin-conjugating enzyme E2K (Ube2k), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ube2k ( NM_001106006.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099476.1 Rattus norvegicus ubiquitin-conjugating enzyme E2K (Ube2k), mRNA.

Price & Availability↑

CloneID	ORa11819
Clone ID Related Accession (Same CDS sequence)	NM_001106006.1
Accession Version	NM_001106006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 K
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473981.1. On or before Oct 4, 2007 this sequence version replaced XM_214043.4, XM_001071289.1. ##Evidence-Data-START## Transcript exon combination :: FQ226880.1, FQ234076.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA 
GGAGAAATAG CAGGACCTCC AGACACACCG TATGAAGGTG GAAGATATCA ACTAGAGATA 
AAAATACCAG AAACATATCC ATTTAACCCC CCTAAGGTCC GGTTTATCAC TAAAATATGG 
CACCCTAATA TTAGTTCCGT CACAGGGGCT ATTTGTTTGG ATATCCTGAA AGATCAATGG 
GCAGCAGCAA TGACTCTGCG CACAGTATTA TTGTCATTGC AAGCACTGCT GGCCGCTGCA 
GAACCAGATG ACCCCCAAGA TGCAGTAGTA GCAAATCAGT ACAAACAAAA TCCTGAAATG 
TTCAAGCAGA CAGCTCGACT TTGGGCACAC GTGTATGCTG GAGCACCAGT GTCTAGTCCA 
GAATACACCA AAAAAATAGA AAACCTCTGT GCTATGGGTT TTGATAGGAA CGCAGTAATA 
GTGGCCTTGT CTTCAAAATC ATGGGATGTA GAGACTGCAA CAGAACTGCT TCTGAGTAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099476.1
CDS	213..815
Translation	MANIAVQRIKREFKEVLKSEETSKNQIKVDLVDENFTELRGEIAGPPDTPYEGGRYQLEIKIPETYPFNPPKVRFITKIWHPNISSVTGAICLDILKDQWAAAMTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN

Target ORF information:

RefSeq Version	NM_001106006.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2K (Ube2k), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106006.1

ORF Insert Sequence:

ATGGCCAACA TCGCGGTGCA GCGAATCAAG CGGGAGTTCA AGGAGGTGCT GAAGAGCGAG 
GAGACGAGCA AAAATCAAAT TAAAGTAGAT CTTGTAGATG AGAATTTTAC AGAATTAAGA 
GGAGAAATAG CAGGACCTCC AGACACACCG TATGAAGGTG GAAGATATCA ACTAGAGATA 
AAAATACCAG AAACATATCC ATTTAACCCC CCTAAGGTCC GGTTTATCAC TAAAATATGG 
CACCCTAATA TTAGTTCCGT CACAGGGGCT ATTTGTTTGG ATATCCTGAA AGATCAATGG 
GCAGCAGCAA TGACTCTGCG CACAGTATTA TTGTCATTGC AAGCACTGCT GGCCGCTGCA 
GAACCAGATG ACCCCCAAGA TGCAGTAGTA GCAAATCAGT ACAAACAAAA TCCTGAAATG 
TTCAAGCAGA CAGCTCGACT TTGGGCACAC GTGTATGCTG GAGCACCAGT GTCTAGTCCA 
GAATACACCA AAAAAATAGA AAACCTCTGT GCTATGGGTT TTGATAGGAA CGCAGTAATA 
GTGGCCTTGT CTTCAAAATC ATGGGATGTA GAGACTGCAA CAGAACTGCT TCTGAGTAAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ube2k ( XM_017599107.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454596.1 Rattus norvegicus ubiquitin-conjugating enzyme E2K (Ube2k), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58246
Clone ID Related Accession (Same CDS sequence)	XM_017599107.1
Accession Version	XM_017599107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin-conjugating enzyme E2 K isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCTGC GCACAGTATT ATTGTCATTG CAAGCACTGC TGGCCGCTGC AGAACCAGAT 
GACCCCCAAG ATGCAGTAGT AGCAAATCAG TACAAACAAA ATCCTGAAAT GTTCAAGCAG 
ACAGCTCGAC TTTGGGCACA CGTGTATGCT GGAGCACCAG TGTCTAGTCC AGAATACACC 
AAAAAAATAG AAAACCTCTG TGCTATGGGT TTTGATAGGA ACGCAGTAAT AGTGGCCTTG 
TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAACTGC TTCTGAGTAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454596.1
CDS	227..520
Translation	MTLRTVLLSLQALLAAAEPDDPQDAVVANQYKQNPEMFKQTARLWAHVYAGAPVSSPEYTKKIENLCAMGFDRNAVIVALSSKSWDVETATELLLSN

Target ORF information:

RefSeq Version	XM_017599107.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin-conjugating enzyme E2K (Ube2k), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017599107.1

ORF Insert Sequence:

ATGACTCTGC GCACAGTATT ATTGTCATTG CAAGCACTGC TGGCCGCTGC AGAACCAGAT 
GACCCCCAAG ATGCAGTAGT AGCAAATCAG TACAAACAAA ATCCTGAAAT GTTCAAGCAG 
ACAGCTCGAC TTTGGGCACA CGTGTATGCT GGAGCACCAG TGTCTAGTCC AGAATACACC 
AAAAAAATAG AAAACCTCTG TGCTATGGGT TTTGATAGGA ACGCAGTAAT AGTGGCCTTG 
TCTTCAAAAT CATGGGATGT AGAGACTGCA ACAGAACTGC TTCTGAGTAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Unanchored K48-linked polyubiquitin synthesized by the E3-ubiquitin ligase TRIM6 stimulates the interferon-IKK? kinase-mediated antiviral response.
Immunity40(6)880-95(2014 Jun)
Rajsbaum R,Versteeg GA,Schmid S,Maestre AM,Belicha-Villanueva A,Mart?nez-Romero C,Patel JR,Morrison J,Pisanelli G,Miorin L,Laurent-Rolle M,Moulton HM,Stein DA,Fernandez-Sesma A,tenOever BR,Garc?a-Sastre A

The E2 ubiquitin-conjugating enzymes direct polyubiquitination to preferred lysines.
The Journal of biological chemistry285(12)8595-604(2010 Mar)
David Y,Ziv T,Admon A,Navon A

Expression of TMEM16 paralogs during murine embryogenesis.
Developmental dynamics : an official publication of the American Association of Anatomists237(9)2566-74(2008 Sep)
Rock JR,Harfe BD

E2-25K/Hip-2 regulates caspase-12 in ER stress-mediated Abeta neurotoxicity.
The Journal of cell biology182(4)675-84(2008 Aug)
Song S,Lee H,Kam TI,Tai ML,Lee JY,Noh JY,Shim SM,Seo SJ,Kong YY,Nakagawa T,Chung CW,Choi DY,Oubrahim H,Jung YK

Characterization of a novel ubiquitin-conjugating enzyme that regulates beta1,4-galactosyltransferase-1 in embryonic stem cells.
Stem cells (Dayton, Ohio)26(8)2006-18(2008 Aug)
Wassler MJ,Shur BD,Zhou W,Geng YJ