Dhps cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dhps
Entrez Gene ID	288923
Full Name	deoxyhypusine synthase
General protein information	Preferred Names deoxyhypusine synthase Names deoxyhypusine synthase DHS
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19q11
Summary	human homolog catalyzes the first step in the post-translational modification of a lysine residue in the precursor eukaryotic initiation factor 5A (eIF5A) to the amino acid hypusine [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001004207.1	NP_001004207.1	deoxyhypusine synthase

Pathways from BioSystems

Homologies

Homo sapiens (human)	DHPS	NP_001921.1
Mus musculus (house mouse)	Dhps	NP_001034603.1
Saccharomyces cerevisiae (baker's yeast)	DYS1	NP_011935.1
Kluyveromyces lactis	KLLA0E12695g	XP_454522.1
Magnaporthe oryzae (rice blast fungus)	MGG_09173	XP_003709783.1
Canis lupus familiaris (dog)	DHPS	XP_533907.1
Oryza sativa (rice)	Os03g0740600	NP_001051218.1
Danio rerio (zebrafish)	dhps	NP_998387.1
Drosophila melanogaster (fruit fly)	CG8005	NP_648188.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006531	XP_316567.4
Eremothecium gossypii	AGOS_AAL036W	NP_982506.1
Neurospora crassa	NCU05418	XP_963241.1
Arabidopsis thaliana (thale cress)	DHS	NP_196211.1
Pan troglodytes (chimpanzee)	DHPS	XP_001169027.1
	DHPS	XP_001108116.1
Bos taurus (cattle)	DHPS	NP_001003657.1
Rattus norvegicus (Norway rat)	Dhps	NP_001004207.1
Danio rerio (zebrafish)	LOC100330342	XP_002661151.2
Schizosaccharomyces pombe (fission yeast)	SPBC1271.04c	NP_595146.1
Caenorhabditis elegans (roundworm)	dhps-1	NP_496557.1
Xenopus tropicalis (tropical clawed frog)	dhps	NP_988906.1

Gene Ontology
Bibliography

Related articles in PubMed

Deoxyhypusine synthase promotes differentiation and proliferation of T helper type 1 (Th1) cells in autoimmune diabetes.
Colvin SC, Maier B, Morris DL, Tersey SA, Mirmira RG
The Journal of biological chemistry288(51)36226-35(2013 Dec)

Allosteric activation of trypanosomatid deoxyhypusine synthase by a catalytically dead paralog.
Nguyen S, Jones DC, Wyllie S, Fairlamb AH, Phillips MA
The Journal of biological chemistry288(21)15256-67(2013 May)

A new crystal structure of deoxyhypusine synthase reveals the configuration of the active enzyme and of an enzyme.NAD.inhibitor ternary complex.
Umland TC, Wolff EC, Park MH, Davies DR
The Journal of biological chemistry279(27)28697-705(2004 Jul)

Enzyme-substrate intermediate formation at lysine 329 of human deoxyhypusine synthase.
Wolff EC, Folk JE, Park MH
The Journal of biological chemistry272(25)15865-71(1997 Jun)

Deoxyhypusine synthase from rat testis: purification and characterization.
Wolff EC, Lee YB, Chung SI, Folk JE, Park MH
The Journal of biological chemistry270(15)8660-6(1995 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dhps gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dhps cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa10070	NM_001004207.1 Latest version!	Rattus norvegicus deoxyhypusine synthase (Dhps), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dhps ( NM_001004207.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001004207.1 Rattus norvegicus deoxyhypusine synthase (Dhps), mRNA.

Price & Availability↑

CloneID	ORa10070
Clone ID Related Accession (Same CDS sequence)	NM_001004207.1
Accession Version	NM_001004207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Rattus norvegicus(Norway rat)
Product	deoxyhypusine synthase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC079188.1. On Sep 10, 2004 this sequence version replaced XM_213837.2. ##Evidence-Data-START## Transcript exon combination :: BC079188.1, FQ214612.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGGGA CCCCGTCGGG GGCGGCGCCC TCCTCGGCGC TGGCCGCTGT GCTCAAGCAC 
AGCTCAGCGT TGCCGCCCGA GAGCGCCCAG GTCCAAGGTT ACGACTTCAA CCGCGGGGTA 
GATTACCACG CGCTTCTGGA AGCCTACGGC ACCACCGGCT TCCAGGCCAC CAACTTCGGG 
CGCGCGGTGC AGCAAGTCAA CGCCATGATT GAGAAGAAGC TGGAACCACT GGCAGTAGAT 
GAAGATCATC ACGAAGACCT GACTCAGAGC CGCCGCCCAC TCACAGGCTG CACCATTTTC 
TTGGGCTATA CGTCCAACCT CATCAGTTCT GGCATCCGGG AGACCATTCG CTACCTCGTG 
CAGCACAATA TGGTGGATGT ACTGGTGACC ACTGCTGGAG GGGTGGAAGA AGATCTCATC 
AAGTGCCTGG CACCCACATA TCTGGGCGAG TTCAGCCTCA GGGGGAAGGA GCTCCGGGAG 
AATGGGATCA ACAGGATCGG GAACCTGCTG GTGCCAAATG ACAATTATTG CAAGTTTGAG 
GACTGGCTCA TGCCCATCCT AGACCAGATG GTGCAGGAGC AGAATACAGA GGGTGTGAAG 
TGGACACCTT CCAAGATGAT CTCCCGGCTT GGGAAGGAGA TCAACAACCC AGAGTCTGTG 
TATTATTGGG CCCATAAGAA CCACATACCT GTGCTGAGTC CTGCACTCAC AGACGGCTCA 
CTGGGTGACA TGATCTTTTT CCATTCCTAT AAAAACCCAG GCTTGGTCCT GGACATCGTT 
GAAGACCTGC GGCTCATCAA CATGCAGGCC ATTTTCGCCA AGCGCACTGG GATGATCATC 
CTGGGTGGAG GCGTGGTCAA GCACCACATC GCCAATGCTA ACCTCATGCG GAATGGAGCT 
GACTACGCTG TTTATATCAA CACAGCCCAG GAGTTTGATG GCTCAGACTC AGGAGCCCGG 
CCAGATGAGG CTGTCTCCTG GGGCAAGATC CGGATGGATG CACAGCCAGT AAAGGTCTAT 
GCTGATGCAT CTCTGGTTTT CCCCTTGCTG GTGGCTGAGA CATTCGCCCA AAAGGCAGAT 
GCCTTCAGAG CTGAGAAGAA TGAGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004207.1
CDS	75..1184
Translation	MEGTPSGAAPSSALAAVLKHSSALPPESAQVQGYDFNRGVDYHALLEAYGTTGFQATNFGRAVQQVNAMIEKKLEPLAVDEDHHEDLTQSRRPLTGCTIFLGYTSNLISSGIRETIRYLVQHNMVDVLVTTAGGVEEDLIKCLAPTYLGEFSLRGKELRENGINRIGNLLVPNDNYCKFEDWLMPILDQMVQEQNTEGVKWTPSKMISRLGKEINNPESVYYWAHKNHIPVLSPALTDGSLGDMIFFHSYKNPGLVLDIVEDLRLINMQAIFAKRTGMIILGGGVVKHHIANANLMRNGADYAVYINTAQEFDGSDSGARPDEAVSWGKIRMDAQPVKVYADASLVFPLLVAETFAQKADAFRAEKNED

Target ORF information:

RefSeq Version	NM_001004207.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus deoxyhypusine synthase (Dhps), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004207.1

ORF Insert Sequence:

ATGGAGGGGA CCCCGTCGGG GGCGGCGCCC TCCTCGGCGC TGGCCGCTGT GCTCAAGCAC 
AGCTCAGCGT TGCCGCCCGA GAGCGCCCAG GTCCAAGGTT ACGACTTCAA CCGCGGGGTA 
GATTACCACG CGCTTCTGGA AGCCTACGGC ACCACCGGCT TCCAGGCCAC CAACTTCGGG 
CGCGCGGTGC AGCAAGTCAA CGCCATGATT GAGAAGAAGC TGGAACCACT GGCAGTAGAT 
GAAGATCATC ACGAAGACCT GACTCAGAGC CGCCGCCCAC TCACAGGCTG CACCATTTTC 
TTGGGCTATA CGTCCAACCT CATCAGTTCT GGCATCCGGG AGACCATTCG CTACCTCGTG 
CAGCACAATA TGGTGGATGT ACTGGTGACC ACTGCTGGAG GGGTGGAAGA AGATCTCATC 
AAGTGCCTGG CACCCACATA TCTGGGCGAG TTCAGCCTCA GGGGGAAGGA GCTCCGGGAG 
AATGGGATCA ACAGGATCGG GAACCTGCTG GTGCCAAATG ACAATTATTG CAAGTTTGAG 
GACTGGCTCA TGCCCATCCT AGACCAGATG GTGCAGGAGC AGAATACAGA GGGTGTGAAG 
TGGACACCTT CCAAGATGAT CTCCCGGCTT GGGAAGGAGA TCAACAACCC AGAGTCTGTG 
TATTATTGGG CCCATAAGAA CCACATACCT GTGCTGAGTC CTGCACTCAC AGACGGCTCA 
CTGGGTGACA TGATCTTTTT CCATTCCTAT AAAAACCCAG GCTTGGTCCT GGACATCGTT 
GAAGACCTGC GGCTCATCAA CATGCAGGCC ATTTTCGCCA AGCGCACTGG GATGATCATC 
CTGGGTGGAG GCGTGGTCAA GCACCACATC GCCAATGCTA ACCTCATGCG GAATGGAGCT 
GACTACGCTG TTTATATCAA CACAGCCCAG GAGTTTGATG GCTCAGACTC AGGAGCCCGG 
CCAGATGAGG CTGTCTCCTG GGGCAAGATC CGGATGGATG CACAGCCAGT AAAGGTCTAT 
GCTGATGCAT CTCTGGTTTT CCCCTTGCTG GTGGCTGAGA CATTCGCCCA AAAGGCAGAT 
GCCTTCAGAG CTGAGAAGAA TGAGGACTGA