Akr1b8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Akr1b8
Entrez Gene ID	286921
Full Name	aldo-keto reductase family 1, member B8
General protein information	Preferred Names aldo-keto reductase family 1, member B8 Names aldose reductase-related protein 2 aldose reductase-like protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q22
Summary	carcinogen-dependent tumor-associated protein [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_173136.1	NP_775159.1	aldose reductase-related protein 2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	CG6084	NP_001261717.1
Magnaporthe oryzae (rice blast fungus)	MGG_03648	XP_003716240.1
Pan troglodytes (chimpanzee)	AKR1B10	XP_001140450.1
Rattus norvegicus (Norway rat)	Akr1b8	NP_775159.1
	AKR1B10	XP_001100959.1
Bos taurus (cattle)	AKR1B10	NP_001095558.1
Mus musculus (house mouse)	Akr1b8	NP_032038.1
Gallus gallus (chicken)	AKR1B10	NP_989960.1
Saccharomyces cerevisiae (baker's yeast)	GRE3	NP_011972.1
Kluyveromyces lactis	KLLA0E21627g	XP_454929.1
Arabidopsis thaliana (thale cress)	AT2G21260	NP_179722.1
Homo sapiens (human)	AKR1B10	NP_064695.3
Eremothecium gossypii	AGOS_ACL107C	NP_983297.1
Neurospora crassa	NCU08384	XP_963931.1
Arabidopsis thaliana (thale cress)	AT2G21250	NP_179721.1
Oryza sativa (rice)	Os02g0123500	NP_001045735.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure of rat aldose reductase-like protein AKR1B14 holoenzyme: Probing the role of His269 in coenzyme binding by site-directed mutagenesis.
Sundaram K, Dhagat U, Endo S, Chung R, Matsunaga T, Hara A, El-Kabbani O
Bioorganic & medicinal chemistry letters21(2)801-4(2011 Jan)

Proteome analysis of rat hepatomas: carcinogen-dependent tumor-associated protein variants.
Zeindl-Eberhart E, Klugbauer S, Dimitrijevic N, Jungblut PR, Lamer S, Rabes HM
Electrophoresis22(14)3009-18(2001 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The crystal structure of the binary complex aldose reductase-like protein-NADPH was determined at 1.86A resolution.
Title: Structure of rat aldose reductase-like protein AKR1B14 holoenzyme: Probing the role of His269 in coenzyme binding by site-directed mutagenesis.

The following Akr1b8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Akr1b8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03531	NM_173136.1 Latest version!	Rattus norvegicus aldo-keto reductase family 1, member B8 (Akr1b8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Akr1b8 ( NM_173136.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_775159.1 Rattus norvegicus aldo-keto reductase family 1, member B8 (Akr1b8), mRNA.

Price & Availability↑

CloneID	ORa03531
Clone ID Related Accession (Same CDS sequence)	NM_173136.1
Accession Version	NM_173136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-26
Organism	Rattus norvegicus(Norway rat)
Product	aldose reductase-related protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ277957.1. ##Evidence-Data-START## Transcript exon combination :: AJ277957.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689594 [ECO:0000348] ##Evidence-Data-END##

ATGGCAACCT TCGTGGAGCT CAGTACCAAA GCCAAGATGC CCATTGTGGG TCTGGGCACC 
TGGAAGTCTA TGCCAAACCA AGTCAAGGAA GCTGTGAAGG CAGCCATTGA TGCTGGGTAT 
CGACATATTG ACTGTGCGTA TGCATATTGC AACGAGAATG AGGTGGGGGA AGCCATCCAA 
GAGAAGATCA AAGAGAAGGC TGTGAGGCGG GAGGACCTCT TCATTGTCAG CAAGTTGTGG 
CCCACCTGCT TTGAGAAAAA ACTGCTAAAG GAAGCCTTTC AGAAGACCCT CACGGATCTC 
AAGCTGGACT ACTTGGACCT CTATCTTATC CACTGGCCTC AGGGATTTCA GGCAGGGAAG 
GAGTTATTCC CCAAAGATGA ACAAGGCAAC GTCCTCCCCA GTAAAACAAC GTTCCTGGAA 
GCCTGGGAGG GCATGGAGGA ACTGGTGGAC CAGGGGCTGG TGAAAGCTCT GGGCGTCTCC 
AACTTCAACC ACTTCCAGAT CGAAAGGCTC CTGAACAAGC CTGGACTGAA ACATAAGCCA 
GTGACCAACC AGGTCGAGTG CCACCCTTAC CTCACCCAGG AAAAACTGAT CCAGTACTGT 
CACTCCAAGG GCATCGTGGT CACAGCCTAC AGTCCCCTGG GCTCCCCAGA CAGGCCTAGG 
GCCAAGCCAG ATGACCCTTC ACTATTACAG GACCCCAAGA TTAAAGAGAT TGCCGCCAAG 
CACAAGAAAA CCACAGCCCA GGTTCTTATT CGGTTCCACA TCCAGAGGAA CGTGGTAGTG 
ATCCCCAAGT CTGTGACGCC AGCACGTATA CAGGAGAACA TTCAGGTCTT TGACTTCCAG 
TTGAGCGACC AGGAGATGGC CACTATCCTG AGCTTCAACA GAAACTGGAG GGCCTGCCTG 
CTGCCTGAGA CAGTCAACAT GGAAGAGTTT CCCTATGACG CAGAGTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775159.1
CDS	43..993
Translation	MATFVELSTKAKMPIVGLGTWKSMPNQVKEAVKAAIDAGYRHIDCAYAYCNENEVGEAIQEKIKEKAVRREDLFIVSKLWPTCFEKKLLKEAFQKTLTDLKLDYLDLYLIHWPQGFQAGKELFPKDEQGNVLPSKTTFLEAWEGMEELVDQGLVKALGVSNFNHFQIERLLNKPGLKHKPVTNQVECHPYLTQEKLIQYCHSKGIVVTAYSPLGSPDRPRAKPDDPSLLQDPKIKEIAAKHKKTTAQVLIRFHIQRNVVVIPKSVTPARIQENIQVFDFQLSDQEMATILSFNRNWRACLLPETVNMEEFPYDAEY

Target ORF information:

RefSeq Version	NM_173136.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus aldo-keto reductase family 1, member B8 (Akr1b8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173136.1

ORF Insert Sequence:

ATGGCAACCT TCGTGGAGCT CAGTACCAAA GCCAAGATGC CCATTGTGGG TCTGGGCACC 
TGGAAGTCTA TGCCAAACCA AGTCAAGGAA GCTGTGAAGG CAGCCATTGA TGCTGGGTAT 
CGACATATTG ACTGTGCGTA TGCATATTGC AACGAGAATG AGGTGGGGGA AGCCATCCAA 
GAGAAGATCA AAGAGAAGGC TGTGAGGCGG GAGGACCTCT TCATTGTCAG CAAGTTGTGG 
CCCACCTGCT TTGAGAAAAA ACTGCTAAAG GAAGCCTTTC AGAAGACCCT CACGGATCTC 
AAGCTGGACT ACTTGGACCT CTATCTTATC CACTGGCCTC AGGGATTTCA GGCAGGGAAG 
GAGTTATTCC CCAAAGATGA ACAAGGCAAC GTCCTCCCCA GTAAAACAAC GTTCCTGGAA 
GCCTGGGAGG GCATGGAGGA ACTGGTGGAC CAGGGGCTGG TGAAAGCTCT GGGCGTCTCC 
AACTTCAACC ACTTCCAGAT CGAAAGGCTC CTGAACAAGC CTGGACTGAA ACATAAGCCA 
GTGACCAACC AGGTCGAGTG CCACCCTTAC CTCACCCAGG AAAAACTGAT CCAGTACTGT 
CACTCCAAGG GCATCGTGGT CACAGCCTAC AGTCCCCTGG GCTCCCCAGA CAGGCCTAGG 
GCCAAGCCAG ATGACCCTTC ACTATTACAG GACCCCAAGA TTAAAGAGAT TGCCGCCAAG 
CACAAGAAAA CCACAGCCCA GGTTCTTATT CGGTTCCACA TCCAGAGGAA CGTGGTAGTG 
ATCCCCAAGT CTGTGACGCC AGCACGTATA CAGGAGAACA TTCAGGTCTT TGACTTCCAG 
TTGAGCGACC AGGAGATGGC CACTATCCTG AGCTTCAACA GAAACTGGAG GGCCTGCCTG 
CTGCCTGAGA CAGTCAACAT GGAAGAGTTT CCCTATGACG CAGAGTACTG A