Pdc cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pdc
Entrez Gene ID	25343
Full Name	phosducin
Synonyms	33DPTP,MEKA,Rpr1
General protein information	Preferred Names phosducin Names phosducin 33 kDa phototransducing protein MEKA protein PHD Phototransducing protein, 33 kDa (phosducin) RPR-1 rod photoreceptor 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q21
Summary	binds the retinal G-protein transducin; plays a role in negative regulation of phototransduction [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012872.1	NP_037004.1	phosducin

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	pdc	XP_002933816.2
Homo sapiens (human)	PDC	NP_002588.3
Canis lupus familiaris (dog)	PDC	NP_001003076.1
Bos taurus (cattle)	PDC	NP_001159999.1
Mus musculus (house mouse)	Pdc	NP_001153202.1
Pan troglodytes (chimpanzee)	PDC	XP_524997.3
Rattus norvegicus (Norway rat)	Pdc	NP_037004.1
Gallus gallus (chicken)	PDC	XP_004943359.1
Danio rerio (zebrafish)	pdcb	NP_001020635.2
	PDC	XP_001107723.2
Danio rerio (zebrafish)	pdca	XP_682823.4

Gene Ontology
Bibliography

Related articles in PubMed

Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function.
Kacirova M, Novacek J, Man P, Obsilova V, Obsil T
Biophysical journal112(7)1339-1349(2017 Apr)

Structural Characterization of Phosducin and Its Complex with the 14-3-3 Protein.
Kacirova M, Kosek D, Kadek A, Man P, Vecer J, Herman P, Obsilova V, Obsil T
The Journal of biological chemistry290(26)16246-60(2015 Jun)

Structural modulation of phosducin by phosphorylation and 14-3-3 protein binding.
Rezabkova L, Kacirova M, Sulc M, Herman P, Vecer J, Stepanek M, Obsilova V, Obsil T
Biophysical journal103(9)1960-9(2012 Nov)

Transducin gamma-subunit sets expression levels of alpha- and beta-subunits and is crucial for rod viability.
Lobanova ES, Finkelstein S, Herrmann R, Chen YM, Kessler C, Michaud NA, Trieu LH, Strissel KJ, Burns ME, Arshavsky VY
The Journal of neuroscience : the official journal of the Society for Neuroscience28(13)3510-20(2008 Mar)

Compartment-specific phosphorylation of phosducin in rods underlies adaptation to various levels of illumination.
Song H, Belcastro M, Young EJ, Sokolov M
The Journal of biological chemistry282(32)23613-21(2007 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

14-3-3 protein-mediated inhibition of Pdc binding to Gtbetagamma is based on steric occlusion of Pdc's Gtbetagamma binding surface.
Title: Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function.

C-terminal domain of Pdc interacts with the outside surface of the 14-3-3 dimer.
Title: Structural Characterization of Phosducin and Its Complex with the 14-3-3 Protein.

14-3-3 phosphorylation of Pdc at Ser-54 and Ser-73 affects the structure of the whole Pdc molecule.
Title: Structural modulation of phosducin by phosphorylation and 14-3-3 protein binding.

phosducin has a role in regulating signaling in the rod outer segment compartment and there are distinct functions for phosphorylation sites 54 and 71
Title: Compartment-specific phosphorylation of phosducin in rods underlies adaptation to various levels of illumination.

data suggest that phosducin action is based on the reduction of transducin affinity to the membranes of rod outer segments, achieved by keeping the transducin beta gamma subunits apart from the alpha subunit
Title: Phosducin facilitates light-driven transducin translocation in rod photoreceptors. Evidence from the phosducin knockout mouse.

The following Pdc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pdc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01175	NM_012872.1 Latest version!	Rattus norvegicus phosducin (Pdc), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pdc ( NM_012872.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037004.1 Rattus norvegicus phosducin (Pdc), mRNA.

Price & Availability↑

CloneID	ORa01175
Clone ID Related Accession (Same CDS sequence)	NM_012872.1
Accession Version	NM_012872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 741bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	phosducin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M33528.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M33528.1, M33530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGAAG CCGCAAGCCA AAGCTTAGAG GAAGATTTTG AAGGACAGGC CACACACACA 
GGACCCAAAG GAGTAATAAA TGACTGGAGA AAGTTTAAAT TAGAAAGTGA AGATGGTGAT 
TCAATTCCAC CCAGCAAGAA GGAGATCCTC AGACAAATGT CCTCTCCTCA GAGCAGAGAT 
GACAAAGACT CGAAAGAAAG AATGAGCAGA AAGATGAGCA TTCAAGAATA TGAACTAATT 
CATCAGGACA AAGAAGATGA AGGTTGCCTT CGCAAATACC GCAGACAGTG CATGCAGGAT 
ATGCATCAGA AGCTGAGCTT TGGGCCTAGG TATGGGTTTG TGTATGAGCT GGAAACAGGG 
GAGCAATTCC TGGAAACCAT CGAAAAGGAG CAGAAGGTCA CCACCATCGT GGTTAACATT 
TACGAGGATG GTGTCAGGGG CTGTGACGCA CTCAACAGCA GTTTAGAATG CCTTGCAGCA 
GAGTACCCAA TGGTCAAGTT CTGTAAAATA AGAGCTTCGA ATACTGGAGC TGGGGACCGC 
TTTTCCTCAG ACGTACTCCC GACATTGCTC ATATACAAAG GTGGGGAGCT CATAAGCAAT 
TTTATTAGTG TCGCTGAACA ATTTGCCGAA GATTTTTTCG CTGCGGATGT GGAGTCTTTC 
CTAAATGAAT ATGGCTTACT ACCAGAAAGA GAGATACATG ACCTAGGGCA GACCAACACG 
GAAGATGAAG ATATCGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037004.1
CDS	82..822
Translation	MEEAASQSLEEDFEGQATHTGPKGVINDWRKFKLESEDGDSIPPSKKEILRQMSSPQSRDDKDSKERMSRKMSIQEYELIHQDKEDEGCLRKYRRQCMQDMHQKLSFGPRYGFVYELETGEQFLETIEKEQKVTTIVVNIYEDGVRGCDALNSSLECLAAEYPMVKFCKIRASNTGAGDRFSSDVLPTLLIYKGGELISNFISVAEQFAEDFFAADVESFLNEYGLLPEREIHDLGQTNTEDEDIE

Target ORF information:

RefSeq Version	NM_012872.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosducin (Pdc), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012872.1

ORF Insert Sequence:

ATGGAAGAAG CCGCAAGCCA AAGCTTAGAG GAAGATTTTG AAGGACAGGC CACACACACA 
GGACCCAAAG GAGTAATAAA TGACTGGAGA AAGTTTAAAT TAGAAAGTGA AGATGGTGAT 
TCAATTCCAC CCAGCAAGAA GGAGATCCTC AGACAAATGT CCTCTCCTCA GAGCAGAGAT 
GACAAAGACT CGAAAGAAAG AATGAGCAGA AAGATGAGCA TTCAAGAATA TGAACTAATT 
CATCAGGACA AAGAAGATGA AGGTTGCCTT CGCAAATACC GCAGACAGTG CATGCAGGAT 
ATGCATCAGA AGCTGAGCTT TGGGCCTAGG TATGGGTTTG TGTATGAGCT GGAAACAGGG 
GAGCAATTCC TGGAAACCAT CGAAAAGGAG CAGAAGGTCA CCACCATCGT GGTTAACATT 
TACGAGGATG GTGTCAGGGG CTGTGACGCA CTCAACAGCA GTTTAGAATG CCTTGCAGCA 
GAGTACCCAA TGGTCAAGTT CTGTAAAATA AGAGCTTCGA ATACTGGAGC TGGGGACCGC 
TTTTCCTCAG ACGTACTCCC GACATTGCTC ATATACAAAG GTGGGGAGCT CATAAGCAAT 
TTTATTAGTG TCGCTGAACA ATTTGCCGAA GATTTTTTCG CTGCGGATGT GGAGTCTTTC 
CTAAATGAAT ATGGCTTACT ACCAGAAAGA GAGATACATG ACCTAGGGCA GACCAACACG 
GAAGATGAAG ATATCGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural Basis for the 14-3-3 Protein-Dependent Inhibition of Phosducin Function.
Biophysical journal112(7)1339-1349(2017 Apr)
Kacirova M,Novacek J,Man P,Obsilova V,Obsil T

Structural Characterization of Phosducin and Its Complex with the 14-3-3 Protein.
The Journal of biological chemistry290(26)16246-60(2015 Jun)
Kacirova M,Kosek D,Kadek A,Man P,Vecer J,Herman P,Obsilova V,Obsil T

Structural modulation of phosducin by phosphorylation and 14-3-3 protein binding.
Biophysical journal103(9)1960-9(2012 Nov)
Rezabkova L,Kacirova M,Sulc M,Herman P,Vecer J,Stepanek M,Obsilova V,Obsil T

Transducin gamma-subunit sets expression levels of alpha- and beta-subunits and is crucial for rod viability.
The Journal of neuroscience : the official journal of the Society for Neuroscience28(13)3510-20(2008 Mar)
Lobanova ES,Finkelstein S,Herrmann R,Chen YM,Kessler C,Michaud NA,Trieu LH,Strissel KJ,Burns ME,Arshavsky VY

Compartment-specific phosphorylation of phosducin in rods underlies adaptation to various levels of illumination.
The Journal of biological chemistry282(32)23613-21(2007 Aug)
Song H,Belcastro M,Young EJ,Sokolov M