Tnp2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tnp2
Entrez Gene ID	24840
Full Name	transition protein 2
General protein information	Preferred Names transition protein 2 Names nuclear transition protein 2 TP-2 TP2 haploid expressed, male germcell specific, chromatin binding
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q11
Summary	a zinc binding nuclear protein in sperm [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017057.2	NP_058753.2	nuclear transition protein 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tnp2	NP_038722.3
Rattus norvegicus (Norway rat)	Tnp2	NP_058753.2

Gene Ontology
Bibliography

Related articles in PubMed

Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells.
Barral S, Morozumi Y, Tanaka H, Montellier E, Govin J, de Dieuleveult M, Charbonnier G, Cout? Y, Puthier D, Buchou T, Boussouar F, Urahama T, Fenaille F, Curtet S, H?ry P, Fernandez-Nunez N, Shiota H, G?rard M, Rousseaux S, Kurumizaka H, Khochbin S
Molecular cell66(1)89-101.e8(2017 Apr)

Acetylation of transition protein 2 (TP2) by KAT3B (p300) alters its DNA condensation property and interaction with putative histone chaperone NPM3.
Pradeepa MM, Nikhil G, Hari Kishore A, Bharath GN, Kundu TK, Rao MR
The Journal of biological chemistry284(43)29956-67(2009 Oct)

Spatiotemporal organization of AT- and GC-rich DNA and their association with transition proteins TP1 and TP2 in rat condensing spermatids.
Kolthur-Seetharam U, Pradeepa MM, Gupta N, Narayanaswamy R, Rao MR
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society57(10)951-62(2009 Oct)

Comparative genomics reveals gene-specific and shared regulatory sequences in the spermatid-expressed mammalian Odf1, Prm1, Prm2, Tnp1, and Tnp2 genes.
Kleene KC, Bagarova J
Genomics92(2)101-6(2008 Aug)

Involvement of importin-4 in the transport of transition protein 2 into the spermatid nucleus.
Pradeepa MM, Manjunatha S, Sathish V, Agrawal S, Rao MR
Molecular and cellular biology28(13)4331-41(2008 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Acetylation of transition protein 2 (TP2) by KAT3B (p300) alters its DNA condensation property and interaction with putative histone chaperone NPM3.
Title: Acetylation of transition protein 2 (TP2) by KAT3B (p300) alters its DNA condensation property and interaction with putative histone chaperone NPM3.

TP1 and TP2 may have concerted functional roles during chromatin remodeling in mammalian spermiogenesis.
Title: Spatiotemporal organization of AT- and GC-rich DNA and their association with transition proteins TP1 and TP2 in rat condensing spermatids.

A detailed analysis to characterize the molecular components underlying the nuclear translocation of TP2, was carried out.
Title: Involvement of importin-4 in the transport of transition protein 2 into the spermatid nucleus.

The following Tnp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tnp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02643	NM_017057.2 Latest version!	Rattus norvegicus transition protein 2 (Tnp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tnp2 ( NM_017057.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058753.2 Rattus norvegicus transition protein 2 (Tnp2), mRNA.

Price & Availability↑

CloneID	ORa02643
Clone ID Related Accession (Same CDS sequence)	NM_017057.2
Accession Version	NM_017057.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	nuclear transition protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078849.1. On Aug 8, 2004 this sequence version replaced NM_017057.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078849.1, X14776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689594, SAMEA2689595 [ECO:0000348] ##Evidence-Data-END##

ATGGACACCA AGATGCAGAG CCTTCCCACC ACTCATCCCC ACCCGCACAG CTCCTCTCGG 
CCTCAAAGTC ACACCAATAA CCAGTGTGCC TGCAGCCACC ACTGCCGGAG CTGCAGCCAA 
GCAGGCCACC CGAGCTCCAG TTCCAGCCCC AGCTCTGGCC CGCCGACGAA GCACCCCAAA 
ACACCCATGC ACTCTCGTTA CTCACCTTCA AGACCCAGCC ACCGCGGCAG CTGCCCCAAG 
AACAGGAAGA CCTTGGAAGG GAAAGTGAGC AAGAGAAAGG CGGTCAGGAG GCGGAAGCGG 
ACTCACAGAG CTAAGAGGCG TAGCTCAGGA CGAAGATACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058753.2
CDS	55..399
Translation	MDTKMQSLPTTHPHPHSSSRPQSHTNNQCACSHHCRSCSQAGHPSSSSSPSSGPPTKHPKTPMHSRYSPSRPSHRGSCPKNRKTLEGKVSKRKAVRRRKRTHRAKRRSSGRRYK

Target ORF information:

RefSeq Version	NM_017057.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transition protein 2 (Tnp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017057.2

ORF Insert Sequence:

ATGGACACCA AGATGCAGAG CCTTCCCACC ACTCATCCCC ACCCGCACAG CTCCTCTCGG 
CCTCAAAGTC ACACCAATAA CCAGTGTGCC TGCAGCCACC ACTGCCGGAG CTGCAGCCAA 
GCAGGCCACC CGAGCTCCAG TTCCAGCCCC AGCTCTGGCC CGCCGACGAA GCACCCCAAA 
ACACCCATGC ACTCTCGTTA CTCACCTTCA AGACCCAGCC ACCGCGGCAG CTGCCCCAAG 
AACAGGAAGA CCTTGGAAGG GAAAGTGAGC AAGAGAAAGG CGGTCAGGAG GCGGAAGCGG 
ACTCACAGAG CTAAGAGGCG TAGCTCAGGA CGAAGATACA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells.
Molecular cell66(1)89-101.e8(2017 Apr)
Barral S,Morozumi Y,Tanaka H,Montellier E,Govin J,de Dieuleveult M,Charbonnier G,Cout? Y,Puthier D,Buchou T,Boussouar F,Urahama T,Fenaille F,Curtet S,H?ry P,Fernandez-Nunez N,Shiota H,G?rard M,Rousseaux S,Kurumizaka H,Khochbin S

Acetylation of transition protein 2 (TP2) by KAT3B (p300) alters its DNA condensation property and interaction with putative histone chaperone NPM3.
The Journal of biological chemistry284(43)29956-67(2009 Oct)
Pradeepa MM,Nikhil G,Hari Kishore A,Bharath GN,Kundu TK,Rao MR

Spatiotemporal organization of AT- and GC-rich DNA and their association with transition proteins TP1 and TP2 in rat condensing spermatids.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society57(10)951-62(2009 Oct)
Kolthur-Seetharam U,Pradeepa MM,Gupta N,Narayanaswamy R,Rao MR

Comparative genomics reveals gene-specific and shared regulatory sequences in the spermatid-expressed mammalian Odf1, Prm1, Prm2, Tnp1, and Tnp2 genes.
Genomics92(2)101-6(2008 Aug)
Kleene KC,Bagarova J

Involvement of importin-4 in the transport of transition protein 2 into the spermatid nucleus.
Molecular and cellular biology28(13)4331-41(2008 Jul)
Pradeepa MM,Manjunatha S,Sathish V,Agrawal S,Rao MR