Sftpa1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sftpa1
Entrez Gene ID	24773
Full Name	surfactant protein A1
Synonyms	PSAP,PSP-A,SP-A,Sftp1,Sftpa,Sftpl
General protein information	Preferred Names surfactant protein A1 Names pulmonary surfactant-associated protein A Surfactant-associated protein 1 (pulmonary surfactant protein, SP-A) pulmonary surfactant-associated protein A-like surfactant, pulmonary-associated protein A1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p14
Summary	binds LPS and modifies LPS-induced immune cell activation in the lung [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001270645.1	NP_001257574.1	pulmonary surfactant-associated protein A isoform 1 precursor
NM_017329.2	NP_059025.2	pulmonary surfactant-associated protein A isoform 2 precursor
NM_001270647.1	NP_001257576.1	pulmonary surfactant-associated protein A isoform 3 precursor

Pathways from BioSystems

Homologies

	SFTPA1	XP_002805703.1
Canis lupus familiaris (dog)	LOC610540	XP_003434532.2
Bos taurus (cattle)	SFTPA1	NP_001071306.2
Gallus gallus (chicken)	LL	NP_001034255.1
Homo sapiens (human)	SFTPA1	NP_001087239.2
Rattus norvegicus (Norway rat)	Sftpa1	NP_001257574.1
Pan troglodytes (chimpanzee)	SFTPA1	XP_001152394.2
Mus musculus (house mouse)	Sftpa1	NP_075623.2

Gene Ontology
Bibliography

Related articles in PubMed

Differential Ligand Binding Specificities of the Pulmonary Collectins Are Determined by the Conformational Freedom of a Surface Loop.
Rynkiewicz MJ, Wu H, Cafarella TR, Nikolaidis NM, Head JF, Seaton BA, McCormack FX
Biochemistry56(31)4095-4105(2017 08)

Expression of Peroxiredoxins and Pulmonary Surfactant Protein A Induced by Silica in Rat Lung Tissue.
Liu N, Xue L, Guan Y, Li QZ, Cao FY, Pang SL, Guan WJ
Biomedical and environmental sciences : BES29(8)584-588(2016 Aug)

Activation of the GLP-1 Receptor by Liraglutide Increases ACE2 Expression, Reversing Right Ventricle Hypertrophy, and Improving the Production of SP-A and SP-B in the Lungs of Type 1 Diabetes Rats.
Roman?-P?rez M, Outeiri?o-Iglesias V, Moya CM, Santisteban P, Gonz?lez-Mat?as LC, Vigo E, Mallo F
Endocrinology156(10)3559-69(2015 Oct)

Mutations of rat surfactant protein A have distinct effects on its glycosylation, secretion, aggregation and degradation.
Yang W, Shen H, Fang G, Li H, Li L, Deng F, Gu W, Li K, Ma L, Gu J, Wang Y
Life sciences117(2)47-55(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPA binds dipalmitoyl-phosphatidylcholine, the major surfactant lipid component, but not phosphatidylinositol; SPD exhibits the opposite preference. Data suggest flexibility in a key surface loop supports distinctive lipid binding of SPA; quadruple mutant SPA (E171D/P175E/R197N/K203D) that introduces SPD-like loop-stabilizing Ca2+ binding site in carbohydrate recognition domain exhibits ligand-binding preferences of SPD.
Title: Differential Ligand Binding Specificities of the Pulmonary Collectins Are Determined by the Conformational Freedom of a Surface Loop.

Downregulation of SP-A expression caused due to silica is an important factor in the occurrence and development of silicosis.
Title: Expression of Peroxiredoxins and Pulmonary Surfactant Protein A Induced by Silica in Rat Lung Tissue.

Liraglutide activates the GLP1 receptor, increasing ACE2 expression, reversing right ventricle hypertrophy, and improving the production of SP-A and SP-B in the lungs of Type 1 Diabetes rats
Title: Activation of the GLP-1 Receptor by Liraglutide Increases ACE2 Expression, Reversing Right Ventricle Hypertrophy, and Improving the Production of SP-A and SP-B in the Lungs of Type 1 Diabetes Rats.

Mutations of rat surfactant protein A have distinct effects on its glycosylation, secretion, aggregation and degradation.
Title: Mutations of rat surfactant protein A have distinct effects on its glycosylation, secretion, aggregation and degradation.

Dual positive effects of leptin were found on protein expression and transcriptional activity of thyroid transcription factor-1
Title: Leptin promotes fetal lung maturity and upregulates SP-A expression in pulmonary alveoli type-II epithelial cells involving TTF-1 activation.

The following Sftpa1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sftpa1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12192	NM_001270645.1 Latest version!	Rattus norvegicus surfactant protein A1 (Sftpa1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa12214	NM_001270647.1 Latest version!	Rattus norvegicus surfactant protein A1 (Sftpa1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa12203	NM_017329.2 Latest version!	Rattus norvegicus surfactant protein A1 (Sftpa1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sftpa1 ( NM_001270645.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257574.1 Rattus norvegicus surfactant protein A1 (Sftpa1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa12192
Clone ID Related Accession (Same CDS sequence)	NM_001270645.1
Accession Version	NM_001270645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	pulmonary surfactant-associated protein A isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC085353.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC085353.1, CK478637.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689595, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGTGGCAGA AGCCACTGGG GATAGTAGCC ATGTCACTGT GTTCTTTGGC CTTCACCCTC 
TTCTTGACTG TTGTCGCTGG TATCAAGTGC AATGTGACAG ACGTTTGTGC TGGAAGCCCT 
GGGATCCCTG GAGCTCCTGG AAACCATGGT CTGCCTGGCA GAGACGGGAG AGACGGTGTC 
AAAGGAGACC CTGGACCTCC AGGTCCCATG GGCCCTCCTG GAGGAATGCC AGGTCTTCCT 
GGACGCGATG GGCTGCCCGG AGCCCCTGGT GCACCTGGAG AACGTGGAGA CAAGGGAGAG 
CCTGGAGAAA GGGGCCTGCC AGGATTTCCA GCTTACCTGG ATGAGGAGCT CCAGACTGAA 
CTCTATGAGA TCAAACATCA GATTCTGCAA ACAATGGGAG TCCTCAGCTT GCAAGGATCC 
ATGCTGTCAG TGGGGGATAA AGTCTTTTCC ACCAATGGGC AGTCAGTCAA CTTTGATACC 
ATTAAAGAGA TGTGTACCAG AGCAGGAGGC AACATTGCTG TCCCGAGGAC TCCTGAGGAG 
AACGAGGCCA TTGCAAGTAT TGCGAAGAAG TACAACAACT ATGTCTACTT GGGCATGATT 
GAAGACCAGA CTCCTGGAGA CTTCCACTAC CTGGATGGGG CTTCTGTGAA CTACACCAAC 
TGGTACCCAG GAGAACCCAG GGGTCAGGGC AAAGAAAAGT GTGTAGAAAT GTATACAGAT 
GGGACATGGA ATGATAGGGG CTGCCTGCAG TACCGGCTGG CTGTTTGTGA ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257574.1
CDS	32..808
Translation	MWQKPLGIVAMSLCSLAFTLFLTVVAGIKCNVTDVCAGSPGIPGAPGNHGLPGRDGRDGVKGDPGPPGPMGPPGGMPGLPGRDGLPGAPGAPGERGDKGEPGERGLPGFPAYLDEELQTELYEIKHQILQTMGVLSLQGSMLSVGDKVFSTNGQSVNFDTIKEMCTRAGGNIAVPRTPEENEAIASIAKKYNNYVYLGMIEDQTPGDFHYLDGASVNYTNWYPGEPRGQGKEKCVEMYTDGTWNDRGCLQYRLAVCEF

Target ORF information:

RefSeq Version	NM_001270645.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus surfactant protein A1 (Sftpa1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270645.1

ORF Insert Sequence:

ATGTGGCAGA AGCCACTGGG GATAGTAGCC ATGTCACTGT GTTCTTTGGC CTTCACCCTC 
TTCTTGACTG TTGTCGCTGG TATCAAGTGC AATGTGACAG ACGTTTGTGC TGGAAGCCCT 
GGGATCCCTG GAGCTCCTGG AAACCATGGT CTGCCTGGCA GAGACGGGAG AGACGGTGTC 
AAAGGAGACC CTGGACCTCC AGGTCCCATG GGCCCTCCTG GAGGAATGCC AGGTCTTCCT 
GGACGCGATG GGCTGCCCGG AGCCCCTGGT GCACCTGGAG AACGTGGAGA CAAGGGAGAG 
CCTGGAGAAA GGGGCCTGCC AGGATTTCCA GCTTACCTGG ATGAGGAGCT CCAGACTGAA 
CTCTATGAGA TCAAACATCA GATTCTGCAA ACAATGGGAG TCCTCAGCTT GCAAGGATCC 
ATGCTGTCAG TGGGGGATAA AGTCTTTTCC ACCAATGGGC AGTCAGTCAA CTTTGATACC 
ATTAAAGAGA TGTGTACCAG AGCAGGAGGC AACATTGCTG TCCCGAGGAC TCCTGAGGAG 
AACGAGGCCA TTGCAAGTAT TGCGAAGAAG TACAACAACT ATGTCTACTT GGGCATGATT 
GAAGACCAGA CTCCTGGAGA CTTCCACTAC CTGGATGGGG CTTCTGTGAA CTACACCAAC 
TGGTACCCAG GAGAACCCAG GGGTCAGGGC AAAGAAAAGT GTGTAGAAAT GTATACAGAT 
GGGACATGGA ATGATAGGGG CTGCCTGCAG TACCGGCTGG CTGTTTGTGA ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sftpa1 ( NM_001270647.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001257576.1 Rattus norvegicus surfactant protein A1 (Sftpa1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	ORa12214
Clone ID Related Accession (Same CDS sequence)	NM_001270647.1
Accession Version	NM_001270647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	pulmonary surfactant-associated protein A isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC085353.1 and CK364490.1. Transcript Variant: This variant (3) has an additional segment in the 5' region and uses a downstream start codon, compared to variant 1. The resulting isoform (3) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CK364490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMN02642807 [ECO:0000348] ##Evidence-Data-END##

ATGTCACTGT GTTCTTTGGC CTTCACCCTC TTCTTGACTG TTGTCGCTGG TATCAAGTGC 
AATGTGACAG ACGTTTGTGC TGGAAGCCCT GGGATCCCTG GAGCTCCTGG AAACCATGGT 
CTGCCTGGCA GAGACGGGAG AGACGGTGTC AAAGGAGACC CTGGACCTCC AGGTCCCATG 
GGCCCTCCTG GAGGAATGCC AGGTCTTCCT GGACGCGATG GGCTGCCCGG AGCCCCTGGT 
GCACCTGGAG AACGTGGAGA CAAGGGAGAG CCTGGAGAAA GGGGCCTGCC AGGATTTCCA 
GCTTACCTGG ATGAGGAGCT CCAGACTGAA CTCTATGAGA TCAAACATCA GATTCTGCAA 
ACAATGGGAG TCCTCAGCTT GCAAGGATCC ATGCTGTCAG TGGGGGATAA AGTCTTTTCC 
ACCAATGGGC AGTCAGTCAA CTTTGATACC ATTAAAGAGA TGTGTACCAG AGCAGGAGGC 
AACATTGCTG TCCCGAGGAC TCCTGAGGAG AACGAGGCCA TTGCAAGTAT TGCGAAGAAG 
TACAACAACT ATGTCTACTT GGGCATGATT GAAGACCAGA CTCCTGGAGA CTTCCACTAC 
CTGGATGGGG CTTCTGTGAA CTACACCAAC TGGTACCCAG GAGAACCCAG GGGTCAGGGC 
AAAGAAAAGT GTGTAGAAAT GTATACAGAT GGGACATGGA ATGATAGGGG CTGCCTGCAG 
TACCGGCTGG CTGTTTGTGA ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257576.1
CDS	273..1019
Translation	MSLCSLAFTLFLTVVAGIKCNVTDVCAGSPGIPGAPGNHGLPGRDGRDGVKGDPGPPGPMGPPGGMPGLPGRDGLPGAPGAPGERGDKGEPGERGLPGFPAYLDEELQTELYEIKHQILQTMGVLSLQGSMLSVGDKVFSTNGQSVNFDTIKEMCTRAGGNIAVPRTPEENEAIASIAKKYNNYVYLGMIEDQTPGDFHYLDGASVNYTNWYPGEPRGQGKEKCVEMYTDGTWNDRGCLQYRLAVCEF

Target ORF information:

RefSeq Version	NM_001270647.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus surfactant protein A1 (Sftpa1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270647.1

ORF Insert Sequence:

ATGTCACTGT GTTCTTTGGC CTTCACCCTC TTCTTGACTG TTGTCGCTGG TATCAAGTGC 
AATGTGACAG ACGTTTGTGC TGGAAGCCCT GGGATCCCTG GAGCTCCTGG AAACCATGGT 
CTGCCTGGCA GAGACGGGAG AGACGGTGTC AAAGGAGACC CTGGACCTCC AGGTCCCATG 
GGCCCTCCTG GAGGAATGCC AGGTCTTCCT GGACGCGATG GGCTGCCCGG AGCCCCTGGT 
GCACCTGGAG AACGTGGAGA CAAGGGAGAG CCTGGAGAAA GGGGCCTGCC AGGATTTCCA 
GCTTACCTGG ATGAGGAGCT CCAGACTGAA CTCTATGAGA TCAAACATCA GATTCTGCAA 
ACAATGGGAG TCCTCAGCTT GCAAGGATCC ATGCTGTCAG TGGGGGATAA AGTCTTTTCC 
ACCAATGGGC AGTCAGTCAA CTTTGATACC ATTAAAGAGA TGTGTACCAG AGCAGGAGGC 
AACATTGCTG TCCCGAGGAC TCCTGAGGAG AACGAGGCCA TTGCAAGTAT TGCGAAGAAG 
TACAACAACT ATGTCTACTT GGGCATGATT GAAGACCAGA CTCCTGGAGA CTTCCACTAC 
CTGGATGGGG CTTCTGTGAA CTACACCAAC TGGTACCCAG GAGAACCCAG GGGTCAGGGC 
AAAGAAAAGT GTGTAGAAAT GTATACAGAT GGGACATGGA ATGATAGGGG CTGCCTGCAG 
TACCGGCTGG CTGTTTGTGA ATTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sftpa1 ( NM_017329.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_059025.2 Rattus norvegicus surfactant protein A1 (Sftpa1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa12203
Clone ID Related Accession (Same CDS sequence)	NM_017329.2
Accession Version	NM_017329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	pulmonary surfactant-associated protein A isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC085353.1 and M33201.1. On Jul 24, 2012 this sequence version replaced NM_017329.1. Transcript Variant: This variant (2) has an alternate splice site in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an internal aa in the N-terminal region, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M33201.1, M15754.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599, SAMN02642611 [ECO:0000348] ##Evidence-Data-END##

ATGTGGAAGC CACTGGGGAT AGTAGCCATG TCACTGTGTT CTTTGGCCTT CACCCTCTTC 
TTGACTGTTG TCGCTGGTAT CAAGTGCAAT GTGACAGACG TTTGTGCTGG AAGCCCTGGG 
ATCCCTGGAG CTCCTGGAAA CCATGGTCTG CCTGGCAGAG ACGGGAGAGA CGGTGTCAAA 
GGAGACCCTG GACCTCCAGG TCCCATGGGC CCTCCTGGAG GAATGCCAGG TCTTCCTGGA 
CGCGATGGGC TGCCCGGAGC CCCTGGTGCA CCTGGAGAAC GTGGAGACAA GGGAGAGCCT 
GGAGAAAGGG GCCTGCCAGG ATTTCCAGCT TACCTGGATG AGGAGCTCCA GACTGAACTC 
TATGAGATCA AACATCAGAT TCTGCAAACA ATGGGAGTCC TCAGCTTGCA AGGATCCATG 
CTGTCAGTGG GGGATAAAGT CTTTTCCACC AATGGGCAGT CAGTCAACTT TGATACCATT 
AAAGAGATGT GTACCAGAGC AGGAGGCAAC ATTGCTGTCC CGAGGACTCC TGAGGAGAAC 
GAGGCCATTG CAAGTATTGC GAAGAAGTAC AACAACTATG TCTACTTGGG CATGATTGAA 
GACCAGACTC CTGGAGACTT CCACTACCTG GATGGGGCTT CTGTGAACTA CACCAACTGG 
TACCCAGGAG AACCCAGGGG TCAGGGCAAA GAAAAGTGTG TAGAAATGTA TACAGATGGG 
ACATGGAATG ATAGGGGCTG CCTGCAGTAC CGGCTGGCTG TTTGTGAATT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_059025.2
CDS	32..805
Translation	MWKPLGIVAMSLCSLAFTLFLTVVAGIKCNVTDVCAGSPGIPGAPGNHGLPGRDGRDGVKGDPGPPGPMGPPGGMPGLPGRDGLPGAPGAPGERGDKGEPGERGLPGFPAYLDEELQTELYEIKHQILQTMGVLSLQGSMLSVGDKVFSTNGQSVNFDTIKEMCTRAGGNIAVPRTPEENEAIASIAKKYNNYVYLGMIEDQTPGDFHYLDGASVNYTNWYPGEPRGQGKEKCVEMYTDGTWNDRGCLQYRLAVCEF

Target ORF information:

RefSeq Version	NM_017329.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus surfactant protein A1 (Sftpa1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017329.2

ORF Insert Sequence:

ATGTGGAAGC CACTGGGGAT AGTAGCCATG TCACTGTGTT CTTTGGCCTT CACCCTCTTC 
TTGACTGTTG TCGCTGGTAT CAAGTGCAAT GTGACAGACG TTTGTGCTGG AAGCCCTGGG 
ATCCCTGGAG CTCCTGGAAA CCATGGTCTG CCTGGCAGAG ACGGGAGAGA CGGTGTCAAA 
GGAGACCCTG GACCTCCAGG TCCCATGGGC CCTCCTGGAG GAATGCCAGG TCTTCCTGGA 
CGCGATGGGC TGCCCGGAGC CCCTGGTGCA CCTGGAGAAC GTGGAGACAA GGGAGAGCCT 
GGAGAAAGGG GCCTGCCAGG ATTTCCAGCT TACCTGGATG AGGAGCTCCA GACTGAACTC 
TATGAGATCA AACATCAGAT TCTGCAAACA ATGGGAGTCC TCAGCTTGCA AGGATCCATG 
CTGTCAGTGG GGGATAAAGT CTTTTCCACC AATGGGCAGT CAGTCAACTT TGATACCATT 
AAAGAGATGT GTACCAGAGC AGGAGGCAAC ATTGCTGTCC CGAGGACTCC TGAGGAGAAC 
GAGGCCATTG CAAGTATTGC GAAGAAGTAC AACAACTATG TCTACTTGGG CATGATTGAA 
GACCAGACTC CTGGAGACTT CCACTACCTG GATGGGGCTT CTGTGAACTA CACCAACTGG 
TACCCAGGAG AACCCAGGGG TCAGGGCAAA GAAAAGTGTG TAGAAATGTA TACAGATGGG 
ACATGGAATG ATAGGGGCTG CCTGCAGTAC CGGCTGGCTG TTTGTGAATT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential Ligand Binding Specificities of the Pulmonary Collectins Are Determined by the Conformational Freedom of a Surface Loop.
Biochemistry56(31)4095-4105(2017 08)
Rynkiewicz MJ,Wu H,Cafarella TR,Nikolaidis NM,Head JF,Seaton BA,McCormack FX

Expression of Peroxiredoxins and Pulmonary Surfactant Protein A Induced by Silica in Rat Lung Tissue.
Biomedical and environmental sciences : BES29(8)584-588(2016 Aug)
Liu N,Xue L,Guan Y,Li QZ,Cao FY,Pang SL,Guan WJ

Activation of the GLP-1 Receptor by Liraglutide Increases ACE2 Expression, Reversing Right Ventricle Hypertrophy, and Improving the Production of SP-A and SP-B in the Lungs of Type 1 Diabetes Rats.
Endocrinology156(10)3559-69(2015 Oct)
Roman?-P?rez M,Outeiri?o-Iglesias V,Moya CM,Santisteban P,Gonz?lez-Mat?as LC,Vigo E,Mallo F

Mutations of rat surfactant protein A have distinct effects on its glycosylation, secretion, aggregation and degradation.
Life sciences117(2)47-55(2014 Nov)
Yang W,Shen H,Fang G,Li H,Li L,Deng F,Gu W,Li K,Ma L,Gu J,Wang Y