RT1-EC2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	RT1-EC2
Entrez Gene ID	24737
Full Name	RT1 class Ib, locus EC2
Synonyms	MHCRTA,RATMHCRTA,RT1-A,RT1-Aw2,RT1.Al,RT1.EC2,RT1Aw2,RTS
General protein information	Preferred Names RT1 class Ib, locus EC2 Names class I histocompatibility antigen, Non-RT1.A alpha-1 chain MHC class I antigen MHC class Ia protein RT1 class Ib gene RT1Aw2 RT1 class Ib gene(Aw2) RT1 class Ib, locus Aw2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Summary	a class Ib gene of the rat major histocompatibility complex [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017588570.1	XP_017444059.1	class I histocompatibility antigen, Non-RT1.A alpha-1 chain
NM_012645.1	NP_036777.1	class I histocompatibility antigen, Non-RT1.A alpha-1 chain precursor

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites.
Hoffert JD, Pisitkun T, Wang G, Shen RF, Knepper MA
Proceedings of the National Academy of Sciences of the United States of America103(18)7159-64(2006 May)

Differential expression of tapasin and immunoproteasome subunits in adenovirus type 5- versus type 12-transformed cells.
Vertegaal AC, Kuiperij HB, Houweling A, Verlaan M, van der Eb AJ, Zantema A
The Journal of biological chemistry278(1)139-46(2003 Jan)

Detection of transcripts for a soluble form of the RT1-E MHC class Ib molecule in rat placenta.
Solier C, McLaren F, Amadou C, Le Bouteiller P, Joly E
Immunogenetics53(4)351-6(2001 May-Jun)

Two different, highly exposed, bulged structures for an unusually long peptide bound to rat MHC class I RT1-Aa.
Speir JA, Stevens J, Joly E, Butcher GW, Wilson IA
Immunity14(1)81-92(2001 Jan)

Physical mapping and evolution of the centromeric class I gene-containing region of the rat MHC.
Walter L, G?nther E
Immunogenetics51(10)829-37(2000 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RT1-EC2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RT1-EC2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00529	NM_012645.1 Latest version!	Rattus norvegicus RT1 class Ib, locus EC2 (RT1-EC2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RT1-EC2 ( NM_012645.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036777.1 Rattus norvegicus RT1 class Ib, locus EC2 (RT1-EC2), mRNA.

Price & Availability↑

CloneID	ORa00529
Clone ID Related Accession (Same CDS sequence)	NM_012645.1
Accession Version	NM_012645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 960bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	class I histocompatibility antigen, Non-RT1.A alpha-1 chain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M31038.1. On Jun 26, 2012 this sequence version replaced XM_003754673.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M31038.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00632251, SAMN00632253 [ECO:0000350] ##Evidence-Data-END##

ATGGGGGCGA TGGCACCGCG CACGCTGCTC CTGCTGCTGG CGGCGGTCCT GGCCCCCACC 
CAGACCTGGG CGGGCTCACA CTCGCTGCGG TATTTCCACA CCGCCGTGTC CCGGCCCGGC 
CTCGGGGAGC CCCGGTTCAT TTCTGTCGGC TACGTGGACG ACACGCAGTT CGTGCGCTAC 
GACAGCGACG CGGAGAATCC GAGATACGAG CCGCGGGCGC GGTGGATGGA GCGGGAGGGG 
CCGGAGTATT GGGAGGAGCA GACACTGGTC GCCAAGGGAC AGGAGCTGGA TTACCGAGTG 
AGCCTGAGGA ACCTGCTAAG CTACTACAAC CAGAGCGAGG GCGGCTCTCA CACCATCCAG 
AGGATGTATG GCTGTGACGT GGGGTCCGAC GGGAGCCTCC TCCGCGGGTA TGAGCAGCAC 
GCCTACGACG GCCGCGATTA CATCGCCCTG AACGAAGACC TGAAAACATG GGCTGTGGCA 
GACTTTGCAG CATGGATCAC CCGGAGCAAG TGGCAGCGGA ATGGTGCTGC AGAGAGAAGC 
AGGGCCTATC TGGAGGGCAC GTGTGTGGAG TGGCTCCTCA GATACCTGGA GCGCGGGAAG 
GAGACGCTGC TGCGCTCAGA TCCCCCAGAG GCACATGTGA CCCTTCACCC CAGACCTGAA 
GGTGATGTGA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGCTGACAT CACCCTGACC 
TGGCAGTTGA ATGGGGAGGA CCTGACCCAG GACATGGAGC TTGTGGAGAC CAGGCCTGCA 
GGGGATGGAA CCTTCCAGAA GTGGGCATCT GTGGTGGTGC CTCTTGGGAA GGAGCAGAAT 
TACACATGCC GTGTGGAGCA TGAGGGGCTG CCTGAGCCCC TTTCCCAGAG ATGGGAGCCT 
TCTCCATCCA CCGACTCCAA CTTACTTCTG TTGTTCTTGG AGCTGTGGCA GTTTTTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036777.1
CDS	10..969
Translation	MGAMAPRTLLLLLAAVLAPTQTWAGSHSLRYFHTAVSRPGLGEPRFISVGYVDDTQFVRYDSDAENPRYEPRARWMEREGPEYWEEQTLVAKGQELDYRVSLRNLLSYYNQSEGGSHTIQRMYGCDVGSDGSLLRGYEQHAYDGRDYIALNEDLKTWAVADFAAWITRSKWQRNGAAERSRAYLEGTCVEWLLRYLERGKETLLRSDPPEAHVTLHPRPEGDVTLRCWALGFYPADITLTWQLNGEDLTQDMELVETRPAGDGTFQKWASVVVPLGKEQNYTCRVEHEGLPEPLSQRWEPSPSTDSNLLLLFLELWQFL

Target ORF information:

RefSeq Version	NM_012645.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RT1 class Ib, locus EC2 (RT1-EC2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012645.1

ORF Insert Sequence:

ATGGGGGCGA TGGCACCGCG CACGCTGCTC CTGCTGCTGG CGGCGGTCCT GGCCCCCACC 
CAGACCTGGG CGGGCTCACA CTCGCTGCGG TATTTCCACA CCGCCGTGTC CCGGCCCGGC 
CTCGGGGAGC CCCGGTTCAT TTCTGTCGGC TACGTGGACG ACACGCAGTT CGTGCGCTAC 
GACAGCGACG CGGAGAATCC GAGATACGAG CCGCGGGCGC GGTGGATGGA GCGGGAGGGG 
CCGGAGTATT GGGAGGAGCA GACACTGGTC GCCAAGGGAC AGGAGCTGGA TTACCGAGTG 
AGCCTGAGGA ACCTGCTAAG CTACTACAAC CAGAGCGAGG GCGGCTCTCA CACCATCCAG 
AGGATGTATG GCTGTGACGT GGGGTCCGAC GGGAGCCTCC TCCGCGGGTA TGAGCAGCAC 
GCCTACGACG GCCGCGATTA CATCGCCCTG AACGAAGACC TGAAAACATG GGCTGTGGCA 
GACTTTGCAG CATGGATCAC CCGGAGCAAG TGGCAGCGGA ATGGTGCTGC AGAGAGAAGC 
AGGGCCTATC TGGAGGGCAC GTGTGTGGAG TGGCTCCTCA GATACCTGGA GCGCGGGAAG 
GAGACGCTGC TGCGCTCAGA TCCCCCAGAG GCACATGTGA CCCTTCACCC CAGACCTGAA 
GGTGATGTGA CCCTGAGGTG CTGGGCCCTG GGCTTCTACC CTGCTGACAT CACCCTGACC 
TGGCAGTTGA ATGGGGAGGA CCTGACCCAG GACATGGAGC TTGTGGAGAC CAGGCCTGCA 
GGGGATGGAA CCTTCCAGAA GTGGGCATCT GTGGTGGTGC CTCTTGGGAA GGAGCAGAAT 
TACACATGCC GTGTGGAGCA TGAGGGGCTG CCTGAGCCCC TTTCCCAGAG ATGGGAGCCT 
TCTCCATCCA CCGACTCCAA CTTACTTCTG TTGTTCTTGG AGCTGTGGCA GTTTTTGTAG