Ldhb cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ldhb
Entrez Gene ID	24534
Full Name	lactate dehydrogenase B
Synonyms	Ldh2
General protein information	Preferred Names lactate dehydrogenase B Names L-lactate dehydrogenase B chain LDH heart subunit LDH-B LDH-H Lactate dehydrogenease B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 4 Map Location: 4q44
Summary	This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have also been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 9. [provided by RefSeq, Feb 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012595.2	NP_036727.1	L-lactate dehydrogenase B chain isoform Ldhb
NM_001316333.1	NP_001303262.1	L-lactate dehydrogenase B chain isoform Ldhbx
NM_001316334.1	NP_001303263.1	L-lactate dehydrogenase B chain isoform Ldhb

Pathways from BioSystems

Homologies

Homo sapiens (human)	LDHB	NP_002291.1
Bos taurus (cattle)	LDHB	NP_776525.1
Danio rerio (zebrafish)	ldhbb	NP_001002474.1
Danio rerio (zebrafish)	ldhba	NP_571322.1
Xenopus tropicalis (tropical clawed frog)	ldhb	NP_988882.1
Pan troglodytes (chimpanzee)	LDHB	NP_001092031.1
	LDHB	XP_001094453.2
Canis lupus familiaris (dog)	LDHB	NP_001239093.1
Rattus norvegicus (Norway rat)	Ldhb	NP_036727.1
Gallus gallus (chicken)	LDHB	NP_989508.1
Mus musculus (house mouse)	Ldhb	NP_032518.1

Gene Ontology
Bibliography

Related articles in PubMed

Ribosomal readthrough at a short UGA stop codon context triggers dual localization of metabolic enzymes in Fungi and animals.
Stiebler AC, Freitag J, Schink KO, Stehlik T, Tillmann BA, Ast J, B?lker M
PLoS genetics10(10)e1004685(2014 Oct)

Lactate dehydrogenase regulation in aged skeletal muscle: Regulation by anabolic steroids and functional overload.
Washington TA, Healey JM, Thompson RW, Lowe LL, Carson JA
Experimental gerontology5766-74(2014 Sep)

Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals.
Schueren F, Lingner T, George R, Hofhuis J, Dickel C, G?rtner J, Thoms S
eLife3e03640(2014 Sep)

Flotillin-1 facilitates toll-like receptor 3 signaling in human endothelial cells.
Fork C, Hitzel J, Nichols BJ, Tikkanen R, Brandes RP
Basic research in cardiology109(6)439(2014)

Circadian rhythm of metabolic oscillation in suprachiasmatic nucleus depends on the mitochondrial oxidation state, reflected by cytochrome C oxidase and lactate dehydrogenase.
Isobe Y, Hida H, Nishino H
Journal of neuroscience research89(6)929-35(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

aging alters aspects of skeletal muscle isoform shift induced by overload and anabolic steroid administration
Title: Lactate dehydrogenase regulation in aged skeletal muscle: Regulation by anabolic steroids and functional overload.

The readthrough-extended lactate dehydrogenase subunit LDHBx can also co-import LDHA, the other LDH subunit, into peroxisomes.
Title: Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals.

LDH and its coding mRNA were higher during the dark period.
Title: Circadian rhythm of metabolic oscillation in suprachiasmatic nucleus depends on the mitochondrial oxidation state, reflected by cytochrome C oxidase and lactate dehydrogenase.

The following Ldhb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ldhb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00415	NM_001316334.1 Latest version!	Rattus norvegicus lactate dehydrogenase B (Ldhb), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa00415	NM_012595.2 Latest version!	Rattus norvegicus lactate dehydrogenase B (Ldhb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00
ORa63208	NM_001316333.1 Latest version!	Rattus norvegicus lactate dehydrogenase B (Ldhb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ldhb ( NM_012595.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_036727.1 Rattus norvegicus lactate dehydrogenase B (Ldhb), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa00415
Clone ID Related Accession (Same CDS sequence)	NM_012595.2 , NM_001316334.1
Accession Version	NM_012595.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Rattus norvegicus(Norway rat)
Product	L-lactate dehydrogenase B chain isoform Ldhb
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FM054345.1, U07181.1 and BU759234.1. On Oct 14, 2015 this sequence version replaced NM_012595.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (Ldhb), which is localized in the cytosol. Variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ212899.1, BC059149.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAACCC TTAAGGAAAA GCTCATTGCG CCAGTCGCAG ACGACGAGAC TGCCGTCCCG 
AACAACAAGA TTACTGTAGT AGGCGTTGGA CAAGTTGGAA TGGCTTGTGC TATCAGCATT 
CTGGGGAAGT CTCTGGCTGA TGAGCTTGCC CTGGTGGATG TCTTGGAAGA CAAGCTCAAA 
GGAGAAATGA TGGATCTGCA GCACGGGAGC TTATTTCTCC AGACTCCGAA AATCGTGGCT 
GATAAAGATT ACTCCGTGAC AGCCAATTCT AAGATTGTGG TGGTGACCGC GGGAGTCCGC 
CAGCAGGAGG GGGAGAGTCG GCTCAACCTG GTGCAGAGAA ACGTCAATGT ATTCAAGTTC 
ATTATTCCTC AGATCGTCAA GTACAGCCCC GACTGCACCA TCATCGTGGT TTCCAACCCA 
GTGGATATTC TTACCTATGT CACCTGGAAG CTGAGCGGGC TACCTAAGCA CCGCGTGATT 
GGAAGTGGAT GCAATCTGGA TTCTGCTCGG TTTCGTTACC TCATGGCCGA AAAGCTTGGT 
ATTCATCCCA GCAGCTGCCA CGGCTGGATC CTGGGCGAGC ACGGGGACTC CAGTGTGGCA 
GTGTGGAGCG GGGTGAATGT GGCAGGAGTC TCCCTCCAGG AACTGAACCC AGAGATGGGA 
ACGGACAATG ACAGCGAGAA CTGGAAGGAG GTGCATAAGA TGGTGGTGGA CAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG CTACACCAAC TGGGCCATCG GCCTAAGTGT GGCTGACCTC 
ATCGAATCCA TGCTGAAAAA CCTCTCTCGG ATTCACCCCG TGTCTACAAT GGTGAAGGGA 
ATGTACGGCA TCGAGAACGA AGTCTTCCTC AGTCTCCCGT GCATCCTTAA TGCTCGGGGA 
CTGACCAGCG TCATCAACCA GAAGCTGAAG GACGATGAGG TCGCTCAGCT CAGGAAGAGT 
GCGGACACCC TGTGGGATAT CCAGAAAGAC CTCAAGGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036727.1
CDS	134..1138
Translation	MATLKEKLIAPVADDETAVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCTIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVDSAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLRKSADTLWDIQKDLKDL

Target ORF information:

RefSeq Version	NM_012595.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lactate dehydrogenase B (Ldhb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012595.2

ORF Insert Sequence:

ATGGCAACCC TTAAGGAAAA GCTCATTGCG CCAGTCGCAG ACGACGAGAC TGCCGTCCCG 
AACAACAAGA TTACTGTAGT AGGCGTTGGA CAAGTTGGAA TGGCTTGTGC TATCAGCATT 
CTGGGGAAGT CTCTGGCTGA TGAGCTTGCC CTGGTGGATG TCTTGGAAGA CAAGCTCAAA 
GGAGAAATGA TGGATCTGCA GCACGGGAGC TTATTTCTCC AGACTCCGAA AATCGTGGCT 
GATAAAGATT ACTCCGTGAC AGCCAATTCT AAGATTGTGG TGGTGACCGC GGGAGTCCGC 
CAGCAGGAGG GGGAGAGTCG GCTCAACCTG GTGCAGAGAA ACGTCAATGT ATTCAAGTTC 
ATTATTCCTC AGATCGTCAA GTACAGCCCC GACTGCACCA TCATCGTGGT TTCCAACCCA 
GTGGATATTC TTACCTATGT CACCTGGAAG CTGAGCGGGC TACCTAAGCA CCGCGTGATT 
GGAAGTGGAT GCAATCTGGA TTCTGCTCGG TTTCGTTACC TCATGGCCGA AAAGCTTGGT 
ATTCATCCCA GCAGCTGCCA CGGCTGGATC CTGGGCGAGC ACGGGGACTC CAGTGTGGCA 
GTGTGGAGCG GGGTGAATGT GGCAGGAGTC TCCCTCCAGG AACTGAACCC AGAGATGGGA 
ACGGACAATG ACAGCGAGAA CTGGAAGGAG GTGCATAAGA TGGTGGTGGA CAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG CTACACCAAC TGGGCCATCG GCCTAAGTGT GGCTGACCTC 
ATCGAATCCA TGCTGAAAAA CCTCTCTCGG ATTCACCCCG TGTCTACAAT GGTGAAGGGA 
ATGTACGGCA TCGAGAACGA AGTCTTCCTC AGTCTCCCGT GCATCCTTAA TGCTCGGGGA 
CTGACCAGCG TCATCAACCA GAAGCTGAAG GACGATGAGG TCGCTCAGCT CAGGAAGAGT 
GCGGACACCC TGTGGGATAT CCAGAAAGAC CTCAAGGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ldhb ( NM_001316334.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001303263.1 Rattus norvegicus lactate dehydrogenase B (Ldhb), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ORa00415
Clone ID Related Accession (Same CDS sequence)	NM_012595.2 , NM_001316334.1
Accession Version	NM_001316334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Rattus norvegicus(Norway rat)
Product	L-lactate dehydrogenase B chain isoform Ldhb
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FM054345.1, CK355620.1, U07181.1 and BU759234.1. On Oct 14, 2015 this sequence version replaced XM_006237615.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (Ldhb). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: FQ212899.1, FQ215687.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAACCC TTAAGGAAAA GCTCATTGCG CCAGTCGCAG ACGACGAGAC TGCCGTCCCG 
AACAACAAGA TTACTGTAGT AGGCGTTGGA CAAGTTGGAA TGGCTTGTGC TATCAGCATT 
CTGGGGAAGT CTCTGGCTGA TGAGCTTGCC CTGGTGGATG TCTTGGAAGA CAAGCTCAAA 
GGAGAAATGA TGGATCTGCA GCACGGGAGC TTATTTCTCC AGACTCCGAA AATCGTGGCT 
GATAAAGATT ACTCCGTGAC AGCCAATTCT AAGATTGTGG TGGTGACCGC GGGAGTCCGC 
CAGCAGGAGG GGGAGAGTCG GCTCAACCTG GTGCAGAGAA ACGTCAATGT ATTCAAGTTC 
ATTATTCCTC AGATCGTCAA GTACAGCCCC GACTGCACCA TCATCGTGGT TTCCAACCCA 
GTGGATATTC TTACCTATGT CACCTGGAAG CTGAGCGGGC TACCTAAGCA CCGCGTGATT 
GGAAGTGGAT GCAATCTGGA TTCTGCTCGG TTTCGTTACC TCATGGCCGA AAAGCTTGGT 
ATTCATCCCA GCAGCTGCCA CGGCTGGATC CTGGGCGAGC ACGGGGACTC CAGTGTGGCA 
GTGTGGAGCG GGGTGAATGT GGCAGGAGTC TCCCTCCAGG AACTGAACCC AGAGATGGGA 
ACGGACAATG ACAGCGAGAA CTGGAAGGAG GTGCATAAGA TGGTGGTGGA CAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG CTACACCAAC TGGGCCATCG GCCTAAGTGT GGCTGACCTC 
ATCGAATCCA TGCTGAAAAA CCTCTCTCGG ATTCACCCCG TGTCTACAAT GGTGAAGGGA 
ATGTACGGCA TCGAGAACGA AGTCTTCCTC AGTCTCCCGT GCATCCTTAA TGCTCGGGGA 
CTGACCAGCG TCATCAACCA GAAGCTGAAG GACGATGAGG TCGCTCAGCT CAGGAAGAGT 
GCGGACACCC TGTGGGATAT CCAGAAAGAC CTCAAGGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303263.1
CDS	222..1226
Translation	MATLKEKLIAPVADDETAVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCTIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVDSAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLRKSADTLWDIQKDLKDL

Target ORF information:

RefSeq Version	NM_001316334.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lactate dehydrogenase B (Ldhb), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316334.1

ORF Insert Sequence:

ATGGCAACCC TTAAGGAAAA GCTCATTGCG CCAGTCGCAG ACGACGAGAC TGCCGTCCCG 
AACAACAAGA TTACTGTAGT AGGCGTTGGA CAAGTTGGAA TGGCTTGTGC TATCAGCATT 
CTGGGGAAGT CTCTGGCTGA TGAGCTTGCC CTGGTGGATG TCTTGGAAGA CAAGCTCAAA 
GGAGAAATGA TGGATCTGCA GCACGGGAGC TTATTTCTCC AGACTCCGAA AATCGTGGCT 
GATAAAGATT ACTCCGTGAC AGCCAATTCT AAGATTGTGG TGGTGACCGC GGGAGTCCGC 
CAGCAGGAGG GGGAGAGTCG GCTCAACCTG GTGCAGAGAA ACGTCAATGT ATTCAAGTTC 
ATTATTCCTC AGATCGTCAA GTACAGCCCC GACTGCACCA TCATCGTGGT TTCCAACCCA 
GTGGATATTC TTACCTATGT CACCTGGAAG CTGAGCGGGC TACCTAAGCA CCGCGTGATT 
GGAAGTGGAT GCAATCTGGA TTCTGCTCGG TTTCGTTACC TCATGGCCGA AAAGCTTGGT 
ATTCATCCCA GCAGCTGCCA CGGCTGGATC CTGGGCGAGC ACGGGGACTC CAGTGTGGCA 
GTGTGGAGCG GGGTGAATGT GGCAGGAGTC TCCCTCCAGG AACTGAACCC AGAGATGGGA 
ACGGACAATG ACAGCGAGAA CTGGAAGGAG GTGCATAAGA TGGTGGTGGA CAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG CTACACCAAC TGGGCCATCG GCCTAAGTGT GGCTGACCTC 
ATCGAATCCA TGCTGAAAAA CCTCTCTCGG ATTCACCCCG TGTCTACAAT GGTGAAGGGA 
ATGTACGGCA TCGAGAACGA AGTCTTCCTC AGTCTCCCGT GCATCCTTAA TGCTCGGGGA 
CTGACCAGCG TCATCAACCA GAAGCTGAAG GACGATGAGG TCGCTCAGCT CAGGAAGAGT 
GCGGACACCC TGTGGGATAT CCAGAAAGAC CTCAAGGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ldhb ( NM_001316333.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001303262.1 Rattus norvegicus lactate dehydrogenase B (Ldhb), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa63208
Clone ID Related Accession (Same CDS sequence)	NM_001316333.1
Accession Version	NM_001316333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Rattus norvegicus(Norway rat)
Product	L-lactate dehydrogenase B chain isoform Ldhbx
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FM054345.1, U07181.1 and BU759234.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (Ldhbx), which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FQ212899.1, BC059149.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAACCC TTAAGGAAAA GCTCATTGCG CCAGTCGCAG ACGACGAGAC TGCCGTCCCG 
AACAACAAGA TTACTGTAGT AGGCGTTGGA CAAGTTGGAA TGGCTTGTGC TATCAGCATT 
CTGGGGAAGT CTCTGGCTGA TGAGCTTGCC CTGGTGGATG TCTTGGAAGA CAAGCTCAAA 
GGAGAAATGA TGGATCTGCA GCACGGGAGC TTATTTCTCC AGACTCCGAA AATCGTGGCT 
GATAAAGATT ACTCCGTGAC AGCCAATTCT AAGATTGTGG TGGTGACCGC GGGAGTCCGC 
CAGCAGGAGG GGGAGAGTCG GCTCAACCTG GTGCAGAGAA ACGTCAATGT ATTCAAGTTC 
ATTATTCCTC AGATCGTCAA GTACAGCCCC GACTGCACCA TCATCGTGGT TTCCAACCCA 
GTGGATATTC TTACCTATGT CACCTGGAAG CTGAGCGGGC TACCTAAGCA CCGCGTGATT 
GGAAGTGGAT GCAATCTGGA TTCTGCTCGG TTTCGTTACC TCATGGCCGA AAAGCTTGGT 
ATTCATCCCA GCAGCTGCCA CGGCTGGATC CTGGGCGAGC ACGGGGACTC CAGTGTGGCA 
GTGTGGAGCG GGGTGAATGT GGCAGGAGTC TCCCTCCAGG AACTGAACCC AGAGATGGGA 
ACGGACAATG ACAGCGAGAA CTGGAAGGAG GTGCATAAGA TGGTGGTGGA CAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG CTACACCAAC TGGGCCATCG GCCTAAGTGT GGCTGACCTC 
ATCGAATCCA TGCTGAAAAA CCTCTCTCGG ATTCACCCCG TGTCTACAAT GGTGAAGGGA 
ATGTACGGCA TCGAGAACGA AGTCTTCCTC AGTCTCCCGT GCATCCTTAA TGCTCGGGGA 
CTGACCAGCG TCATCAACCA GAAGCTGAAG GACGATGAGG TCGCTCAGCT CAGGAAGAGT 
GCGGACACCC TGTGGGATAT CCAGAAAGAC CTCAAGGACC TGTGACTGCC AGGCGCCAGG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303262.1
CDS	134..1159
Translation	MATLKEKLIAPVADDETAVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCTIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVDSAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLRKSADTLWDIQKDLKDL*LPGARL

Target ORF information:

RefSeq Version	NM_001316333.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lactate dehydrogenase B (Ldhb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316333.1

ORF Insert Sequence:

ATGGCAACCC TTAAGGAAAA GCTCATTGCG CCAGTCGCAG ACGACGAGAC TGCCGTCCCG 
AACAACAAGA TTACTGTAGT AGGCGTTGGA CAAGTTGGAA TGGCTTGTGC TATCAGCATT 
CTGGGGAAGT CTCTGGCTGA TGAGCTTGCC CTGGTGGATG TCTTGGAAGA CAAGCTCAAA 
GGAGAAATGA TGGATCTGCA GCACGGGAGC TTATTTCTCC AGACTCCGAA AATCGTGGCT 
GATAAAGATT ACTCCGTGAC AGCCAATTCT AAGATTGTGG TGGTGACCGC GGGAGTCCGC 
CAGCAGGAGG GGGAGAGTCG GCTCAACCTG GTGCAGAGAA ACGTCAATGT ATTCAAGTTC 
ATTATTCCTC AGATCGTCAA GTACAGCCCC GACTGCACCA TCATCGTGGT TTCCAACCCA 
GTGGATATTC TTACCTATGT CACCTGGAAG CTGAGCGGGC TACCTAAGCA CCGCGTGATT 
GGAAGTGGAT GCAATCTGGA TTCTGCTCGG TTTCGTTACC TCATGGCCGA AAAGCTTGGT 
ATTCATCCCA GCAGCTGCCA CGGCTGGATC CTGGGCGAGC ACGGGGACTC CAGTGTGGCA 
GTGTGGAGCG GGGTGAATGT GGCAGGAGTC TCCCTCCAGG AACTGAACCC AGAGATGGGA 
ACGGACAATG ACAGCGAGAA CTGGAAGGAG GTGCATAAGA TGGTGGTGGA CAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG CTACACCAAC TGGGCCATCG GCCTAAGTGT GGCTGACCTC 
ATCGAATCCA TGCTGAAAAA CCTCTCTCGG ATTCACCCCG TGTCTACAAT GGTGAAGGGA 
ATGTACGGCA TCGAGAACGA AGTCTTCCTC AGTCTCCCGT GCATCCTTAA TGCTCGGGGA 
CTGACCAGCG TCATCAACCA GAAGCTGAAG GACGATGAGG TCGCTCAGCT CAGGAAGAGT 
GCGGACACCC TGTGGGATAT CCAGAAAGAC CTCAAGGACC TGTGACTGCC AGGCGCCAGG 
CTGTAG