Gpha2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Gpha2
Entrez Gene ID	171158
Full Name	glycoprotein hormone alpha 2
Synonyms	Gpa2,Zsig51
General protein information	Preferred Names glycoprotein hormone alpha 2 Names glycoprotein hormone alpha-2 cysteine knot protein glycoprotein-alpha 2 putative secreted protein ZSIG51 thyrostimulin subunit alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q43
Summary	expressed in pancreatic acinar cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008760070.2	XP_008758292.1	glycoprotein hormone alpha-2 isoform X1
NM_133619.2	NP_598303.1	glycoprotein hormone alpha-2 precursor
XM_008760069.2	XP_008758291.1	glycoprotein hormone alpha-2 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GPHA2	NP_570125.1
Pan troglodytes (chimpanzee)	GPHA2	XP_001167465.1
Bos taurus (cattle)	GPHA2	NP_001180095.1
Rattus norvegicus (Norway rat)	LOC100909513	XP_006231018.1
Danio rerio (zebrafish)	gpha2	NP_001160156.1
Rattus norvegicus (Norway rat)	Gpha2	NP_598303.1
Mus musculus (house mouse)	Gpha2	NP_569720.1
Xenopus tropicalis (tropical clawed frog)	gpha2	XP_002937557.1
	GPHA2	XP_001114717.2

Gene Ontology
Bibliography

Related articles in PubMed

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Hasson SA, Kane LA, Yamano K, Huang CH, Sliter DA, Buehler E, Wang C, Heman-Ackah SM, Hessa T, Guha R, Martin SE, Youle RJ
Nature504(7479)291-5(2013 Dec)

Thyrostimulin, but not thyroid-stimulating hormone (TSH), acts as a paracrine regulator to activate the TSH receptor in mammalian ovary.
Sun SC, Hsu PJ, Wu FJ, Li SH, Lu CH, Luo CW
The Journal of biological chemistry285(6)3758-65(2010 Feb)

Differential expression of the thyrostimulin subunits, glycoprotein alpha2 and beta5 in the rat pituitary.
Nagasaki H, Wang Z, Jackson VR, Lin S, Nothacker HP, Civelli O
Journal of molecular endocrinology37(1)39-50(2006 Aug)

Distribution of thyrostimulin in the rat: an immunohistochemical study.
Li C, Hirooka Y, Habu S, Takagi J, Gotoh M, Nogimori T
Endocrine regulations38(4)131-42(2004 Dec)

Evolution of glycoprotein hormone subunit genes in bilateral metazoa: identification of two novel human glycoprotein hormone subunit family genes, GPA2 and GPB5.
Hsu SY, Nakabayashi K, Bhalla A
Molecular endocrinology (Baltimore, Md.)16(7)1538-51(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that the presence of thyrostimulin in the ovary suggests that it may have a primitive function in reproduction when it activates ovarian TSHR.
Title: Thyrostimulin, but not thyroid-stimulating hormone (TSH), acts as a paracrine regulator to activate the TSH receptor in mammalian ovary.

Results describe the differential expression of the thyrostimulin subunits, glycoprotein alpha2 and beta5 in the rat pituitary, and suggest that the thyrostimulin system is functionally different from the thyroid-stimulating hormone system.
Title: Differential expression of the thyrostimulin subunits, glycoprotein alpha2 and beta5 in the rat pituitary.

Thyrostimulin immunoreactivity was visualized in the anterior pituitary, central nervous system, adrenal gland, stomach, duodenum, pancreas and testis; findings suggest that thyrostimulin is widely distributed [thyrostimulin]
Title: Distribution of thyrostimulin in the rat: an immunohistochemical study.

The following Gpha2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Gpha2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa23861	NM_133619.2 Latest version!	Rattus norvegicus glycoprotein hormone subunit alpha 2 (Gpha2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa23861	XM_008760069.2 Latest version!	Rattus norvegicus glycoprotein hormone alpha 2 (Gpha2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa23861	XM_008760070.2 Latest version!	Rattus norvegicus glycoprotein hormone alpha 2 (Gpha2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Gpha2 ( NM_133619.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_598303.1 Rattus norvegicus glycoprotein hormone subunit alpha 2 (Gpha2), mRNA.

Price & Availability↑

CloneID	ORa23861
Clone ID Related Accession (Same CDS sequence)	XM_008760070.2 , NM_133619.2 , XM_008760069.2
Accession Version	NM_133619.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-27
Organism	Rattus norvegicus(Norway rat)
Product	glycoprotein hormone alpha-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF260741.1. On Nov 2, 2002 this sequence version replaced NM_133619.1. ##Evidence-Data-START## Transcript exon combination :: AF260741.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMN02642676 [ECO:0000348] ##Evidence-Data-END##

ATGCCCATGG CACCTCGAGT CTTGCTCTTC TGCCTGCTGG GTCTGGCAGT CACTGAAGGG 
CATGGCCTGG AGGCAGCCGT CCCAATCCCA GGCTGCCACT TGCACCCCTT TAACGTGACA 
GTGCGAAGTG ATCGCCATGG CACCTGCCAG GGCTCCCATG TGGCACAGGC GTGTGTAGGA 
CACTGTGAGT CTAGTGCTTT CCCTTCTCGG TACTCTGTGC TGGTTGCCAG TGGCTATCGA 
CACAACATCA CCTCTGTCTC TCAGTGCTGT ACCATCAGCA GCCTTAAAAA GGTGAGGGTG 
TGGCTGCACT GCGTGGGGAA CCAGCGTGGG GAGCTCGAGA TCTTCACGGC TAGGGCCTGC 
CAGTGTGATA TGTGCCGTCT CTCCCGCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598303.1
CDS	203..595
Translation	MPMAPRVLLFCLLGLAVTEGHGLEAAVPIPGCHLHPFNVTVRSDRHGTCQGSHVAQACVGHCESSAFPSRYSVLVASGYRHNITSVSQCCTISSLKKVRVWLHCVGNQRGELEIFTARACQCDMCRLSRY

Target ORF information:

RefSeq Version	NM_133619.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glycoprotein hormone subunit alpha 2 (Gpha2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133619.2

ORF Insert Sequence:

ATGCCCATGG CACCTCGAGT CTTGCTCTTC TGCCTGCTGG GTCTGGCAGT CACTGAAGGG 
CATGGCCTGG AGGCAGCCGT CCCAATCCCA GGCTGCCACT TGCACCCCTT TAACGTGACA 
GTGCGAAGTG ATCGCCATGG CACCTGCCAG GGCTCCCATG TGGCACAGGC GTGTGTAGGA 
CACTGTGAGT CTAGTGCTTT CCCTTCTCGG TACTCTGTGC TGGTTGCCAG TGGCTATCGA 
CACAACATCA CCTCTGTCTC TCAGTGCTGT ACCATCAGCA GCCTTAAAAA GGTGAGGGTG 
TGGCTGCACT GCGTGGGGAA CCAGCGTGGG GAGCTCGAGA TCTTCACGGC TAGGGCCTGC 
CAGTGTGATA TGTGCCGTCT CTCCCGCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpha2 ( XM_008760070.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758292.1 Rattus norvegicus glycoprotein hormone alpha 2 (Gpha2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa23861
Clone ID Related Accession (Same CDS sequence)	XM_008760070.2 , NM_133619.2 , XM_008760069.2
Accession Version	XM_008760070.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glycoprotein hormone alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760070.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATGG CACCTCGAGT CTTGCTCTTC TGCCTGCTGG GTCTGGCAGT CACTGAAGGG 
CATGGCCTGG AGGCAGCCGT CCCAATCCCA GGCTGCCACT TGCACCCCTT TAACGTGACA 
GTGCGAAGTG ATCGCCATGG CACCTGCCAG GGCTCCCATG TGGCACAGGC GTGTGTAGGA 
CACTGTGAGT CTAGTGCTTT CCCTTCTCGG TACTCTGTGC TGGTTGCCAG TGGCTATCGA 
CACAACATCA CCTCTGTCTC TCAGTGCTGT ACCATCAGCA GCCTTAAAAA GGTGAGGGTG 
TGGCTGCACT GCGTGGGGAA CCAGCGTGGG GAGCTCGAGA TCTTCACGGC TAGGGCCTGC 
CAGTGTGATA TGTGCCGTCT CTCCCGCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758292.1
CDS	2077..2469
Translation	MPMAPRVLLFCLLGLAVTEGHGLEAAVPIPGCHLHPFNVTVRSDRHGTCQGSHVAQACVGHCESSAFPSRYSVLVASGYRHNITSVSQCCTISSLKKVRVWLHCVGNQRGELEIFTARACQCDMCRLSRY

Target ORF information:

RefSeq Version	XM_008760070.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glycoprotein hormone alpha 2 (Gpha2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760070.2

ORF Insert Sequence:

ATGCCCATGG CACCTCGAGT CTTGCTCTTC TGCCTGCTGG GTCTGGCAGT CACTGAAGGG 
CATGGCCTGG AGGCAGCCGT CCCAATCCCA GGCTGCCACT TGCACCCCTT TAACGTGACA 
GTGCGAAGTG ATCGCCATGG CACCTGCCAG GGCTCCCATG TGGCACAGGC GTGTGTAGGA 
CACTGTGAGT CTAGTGCTTT CCCTTCTCGG TACTCTGTGC TGGTTGCCAG TGGCTATCGA 
CACAACATCA CCTCTGTCTC TCAGTGCTGT ACCATCAGCA GCCTTAAAAA GGTGAGGGTG 
TGGCTGCACT GCGTGGGGAA CCAGCGTGGG GAGCTCGAGA TCTTCACGGC TAGGGCCTGC 
CAGTGTGATA TGTGCCGTCT CTCCCGCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gpha2 ( XM_008760069.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758291.1 Rattus norvegicus glycoprotein hormone alpha 2 (Gpha2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23861
Clone ID Related Accession (Same CDS sequence)	XM_008760070.2 , NM_133619.2 , XM_008760069.2
Accession Version	XM_008760069.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	glycoprotein hormone alpha-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760069.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCATGG CACCTCGAGT CTTGCTCTTC TGCCTGCTGG GTCTGGCAGT CACTGAAGGG 
CATGGCCTGG AGGCAGCCGT CCCAATCCCA GGCTGCCACT TGCACCCCTT TAACGTGACA 
GTGCGAAGTG ATCGCCATGG CACCTGCCAG GGCTCCCATG TGGCACAGGC GTGTGTAGGA 
CACTGTGAGT CTAGTGCTTT CCCTTCTCGG TACTCTGTGC TGGTTGCCAG TGGCTATCGA 
CACAACATCA CCTCTGTCTC TCAGTGCTGT ACCATCAGCA GCCTTAAAAA GGTGAGGGTG 
TGGCTGCACT GCGTGGGGAA CCAGCGTGGG GAGCTCGAGA TCTTCACGGC TAGGGCCTGC 
CAGTGTGATA TGTGCCGTCT CTCCCGCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758291.1
CDS	2235..2627
Translation	MPMAPRVLLFCLLGLAVTEGHGLEAAVPIPGCHLHPFNVTVRSDRHGTCQGSHVAQACVGHCESSAFPSRYSVLVASGYRHNITSVSQCCTISSLKKVRVWLHCVGNQRGELEIFTARACQCDMCRLSRY

Target ORF information:

RefSeq Version	XM_008760069.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glycoprotein hormone alpha 2 (Gpha2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760069.2

ORF Insert Sequence:

ATGCCCATGG CACCTCGAGT CTTGCTCTTC TGCCTGCTGG GTCTGGCAGT CACTGAAGGG 
CATGGCCTGG AGGCAGCCGT CCCAATCCCA GGCTGCCACT TGCACCCCTT TAACGTGACA 
GTGCGAAGTG ATCGCCATGG CACCTGCCAG GGCTCCCATG TGGCACAGGC GTGTGTAGGA 
CACTGTGAGT CTAGTGCTTT CCCTTCTCGG TACTCTGTGC TGGTTGCCAG TGGCTATCGA 
CACAACATCA CCTCTGTCTC TCAGTGCTGT ACCATCAGCA GCCTTAAAAA GGTGAGGGTG 
TGGCTGCACT GCGTGGGGAA CCAGCGTGGG GAGCTCGAGA TCTTCACGGC TAGGGCCTGC 
CAGTGTGATA TGTGCCGTCT CTCCCGCTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-content genome-wide RNAi screens identify regulators of parkin upstream of mitophagy.
Nature504(7479)291-5(2013 Dec)
Hasson SA,Kane LA,Yamano K,Huang CH,Sliter DA,Buehler E,Wang C,Heman-Ackah SM,Hessa T,Guha R,Martin SE,Youle RJ

Thyrostimulin, but not thyroid-stimulating hormone (TSH), acts as a paracrine regulator to activate the TSH receptor in mammalian ovary.
The Journal of biological chemistry285(6)3758-65(2010 Feb)
Sun SC,Hsu PJ,Wu FJ,Li SH,Lu CH,Luo CW

Differential expression of the thyrostimulin subunits, glycoprotein alpha2 and beta5 in the rat pituitary.
Journal of molecular endocrinology37(1)39-50(2006 Aug)
Nagasaki H,Wang Z,Jackson VR,Lin S,Nothacker HP,Civelli O

Distribution of thyrostimulin in the rat: an immunohistochemical study.
Endocrine regulations38(4)131-42(2004 Dec)
Li C,Hirooka Y,Habu S,Takagi J,Gotoh M,Nogimori T

Evolution of glycoprotein hormone subunit genes in bilateral metazoa: identification of two novel human glycoprotein hormone subunit family genes, GPA2 and GPB5.
Molecular endocrinology (Baltimore, Md.)16(7)1538-51(2002 Jul)
Hsu SY,Nakabayashi K,Bhalla A