Slc5a5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc5a5
Entrez Gene ID	114613
Full Name	solute carrier family 5 member 5
Synonyms	Nis
General protein information	Preferred Names solute carrier family 5 member 5 Names sodium/iodide cotransporter Na(+)/I(-) cotransporter Na(+)/I(-) symporter Na+/I- symporter sodium/iodide symporter solute carrier family 5 (sodium iodide symporter), member 5 solute carrier family 5 (sodium/iodide cotransporter), member 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p14
Summary	catalyzes Na+/I- symporter activity; plays a role in iodide transport and thyroid hormone generation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_052983.2	NP_443215.2	sodium/iodide cotransporter

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SLC5A5	XP_541946.3
Xenopus tropicalis (tropical clawed frog)	slc5a5	NP_001116937.1
Homo sapiens (human)	SLC5A5	NP_000444.1
Bos taurus (cattle)	SLC5A5	XP_002688618.2
Mus musculus (house mouse)	Slc5a5	NP_444478.2
Rattus norvegicus (Norway rat)	Slc5a5	NP_443215.2
Danio rerio (zebrafish)	slc5a5	NP_001082860.1
Pan troglodytes (chimpanzee)	SLC5A5	XP_524154.2
Gallus gallus (chicken)	SLC5A5	XP_429095.4

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of thyroid sodium-iodide symporter in different stages of goiter: Possible involvement of reactive oxygen species.
Matos LPL, Penha RCC, Cardoso-Weide LC, Freitas ML, Silva DLSG, Ferreira ACF
Clinical and experimental pharmacology & physiology(2017 Nov)

Excess iodide downregulates Na(+)/I(-) symporter gene transcription through activation of PI3K/Akt pathway.
Serrano-Nascimento C, Nicola JP, Teixeira Sda S, Poyares LL, Lellis-Santos C, Bordin S, Masini-Repiso AM, Nunes MT
Molecular and cellular endocrinology42673-90(2016 May)

Selenium Increases Thyroid-Stimulating Hormone-Induced Sodium/Iodide Symporter Expression Through Thioredoxin/Apurinic/Apyrimidinic Endonuclease 1-Dependent Regulation of Paired Box 8 Binding Activity.
Leoni SG, Sastre-Perona A, De la Vieja A, Santisteban P
Antioxidants & redox signaling24(15)855-66(2016 May)

Beyond non-integer Hill coefficients: A novel approach to analyzing binding data, applied to Na+-driven transporters.
Ravera S, Quick M, Nicola JP, Carrasco N, Amzel LM
The Journal of general physiology145(6)555-63(2015 Jun)

Feasibility of lentiviral?mediated sodium iodide symporter gene delivery for the efficient monitoring of bone marrow?derived mesenchymal stem cell transplantation and survival.
Shi S, Zhang M, Guo R, Miao Y, Zhang M, Hu J, Xi Y, Li B
International journal of molecular medicine34(6)1547-54(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

excess iodide transcriptionally represses NIS gene expression through the impairment of Pax8 and p65 transcriptional activity.
Title: Excess iodide downregulates Na(+)/I(-) symporter gene transcription through activation of PI3K/Akt pathway.

Nis expression is controlled by Txn/Ape1 through a TSH/selenium-dependent mechanism.
Title: Selenium Increases Thyroid-Stimulating Hormone-Induced Sodium/Iodide Symporter Expression Through Thioredoxin/Apurinic/Apyrimidinic Endonuclease 1-Dependent Regulation of Paired Box 8 Binding Activity.

Report sodium binding date for NIS, where transporter activity is electrogenic, with a 2:1 Na(+)/I(-) transport stoichiometry.
Title: Beyond non-integer Hill coefficients: A novel approach to analyzing binding data, applied to Na+-driven transporters.

It is feasible to use lentiviral vectors to deliver sodium iodide symporter (NIS) gene for the non-invasive monitoring of bone marrowderived mesenchymal stem cell transplantation and survival in the infarcted myocardium in vivo.
Title: Feasibility of lentiviral?mediated sodium iodide symporter gene delivery for the efficient monitoring of bone marrow?derived mesenchymal stem cell transplantation and survival.

Although the affinity of NIS for I- is low, it increases when Na+ is bound. NIS takes advantage of the extracellular Na+ concentration and the pronounced increase in its own affinity for I- and for the second Na+ elicited by binding of the first.
Title: Physiological sodium concentrations enhance the iodide affinity of the Na+/I- symporter.

The following Slc5a5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc5a5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13257	NM_052983.2 Latest version!	Rattus norvegicus solute carrier family 5 member 5 (Slc5a5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$363.30 $519.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc5a5 ( NM_052983.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_443215.2 Rattus norvegicus solute carrier family 5 member 5 (Slc5a5), mRNA.

Price & Availability↑

CloneID	ORa13257
Clone ID Related Accession (Same CDS sequence)	NM_052983.2
Accession Version	NM_052983.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Rattus norvegicus(Norway rat)
Product	sodium/iodide cotransporter
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from U60282.1, AABR07024873.1 and BF392663.1. On Oct 11, 2007 this sequence version replaced NM_052983.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: KP213324.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00632252, SAMN00632253 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGTG CGGAGGCCGG GGCCCGGGCC ACCTTCGGCG CCTGGGACTA CGGCGTGTTC 
GCGACCATGC TGCTGGTGTC CACGGGCATC GGGCTATGGG TCGGCCTGGC CCGCGGTGGC 
CAACGCAGTG CCGACGACTT CTTTACCGGG GGCCGGCAGT TGGCAGCCGT TCCTGTGGGG 
CTGTCGCTGG CCGCCAGTTT CATGTCGGCT GTGCAGGTGC TCGGGGTCCC CGCCGAGGCA 
GCGCGCTACG GGCTCAAGTT CCTGTGGATG TGCGCGGGTC AGTTGCTCAA CTCGCTGCTC 
ACAGCGTTTC TCTTCTTGCC GATCTTCTAC CGCCTGGGCC TTACCAGCAC CTACCAGTAC 
CTAGAGCTGC GCTTCAGCCG AGCGGTCCGG CTCTGCGGGA CGCTGCAGTA CTTGGTGGCC 
ACGATGCTGT ATACAGGCAT CGTGATCTAC GCGCCTGCGC TCATCCTGAA CCAAGTGACC 
GGGTTGGACA TCTGGGCATC GCTCCTGTCC ACAGGAATCA TCTGCACCTT GTACACTACC 
GTGGGTGGTA TGAAGGCCGT GGTCTGGACA GATGTGTTCC AGGTTGTGGT AATGCTCGTT 
GGCTTCTGGG TGATCCTGGC CCGAGGCGTC ATTCTCCTGG GGGGTCCCCG GAACATGCTC 
AGCCTCGCTC AGAACCATTC CCGGATCAAC CTGATGGACT TTGACCCTGA TCCTCGGAGC 
CGGTACACCT TCTGGACTTT CATAGTGGGT GGCACACTGG TGTGGCTCTC CATGTACGGT 
GTGAACCAAG CCCAGGTACA GCGCTATGTG GCCTGCCACA CAGAGGGAAA GGCCAAACTG 
GCCCTGCTTG TCAACCAGCT GGGCCTCTTC CTGATTGTGG CCAGTGCAGC TTGCTGTGGC 
ATTGTCATGT TCGTCTACTA CAAGGACTGT GACCCCCTCC TCACAGGCCG TATCTCAGCC 
CCCGACCAGT ACATGCCGCT GCTTGTGTTG GACATTTTTG AGGATCTGCC CGGAGTCCCC 
GGGCTCTTCC TGGCCTGTGC CTACAGTGGC ACCCTCAGCA CTGCATCCAC CAGCATCAAC 
GCCATGGCAG CTGTGACTGT GGAAGACCTC ATCAAGCCGA GGATGCCTGG CCTGGCACCT 
CGGAAGTTGG TTTTCATCTC TAAAGGGCTC TCATTCATCT ACGGCTCTGC CTGCCTCACT 
GTGGCTGCTC TGTCCTCACT GCTGGGAGGT GGTGTCCTCC AGGGTTCCTT CACTGTGATG 
GGTGTCATCA GTGGGCCTCT ACTAGGCGCC TTCACGCTTG GGATGCTGCT CCCAGCCTGC 
AACACGCCAG GCGTTCTCTC CGGGTTGGCA GCAGGCTTGG CTGTATCCCT GTGGGTGGCC 
GTAGGGGCCA CACTGTATCC CCCTGGAGAG CAGACCATGG GGGTGCTGCC CACCTCGGCT 
GCAGGCTGCA CCAACGATTC GGTCCTCCTG GGCCCACCTG GAGCCACCAA CGCTTCCAAC 
GGGATCCCCA GTTCTGGAAT GGACACGGGC CGCCCTGCCC TCGCTGATAC CTTTTACGCC 
ATCTCCTATC TCTATTACGG GGCTCTGGGC ACGCTGACCA CCATGCTTTG CGGTGCTCTC 
ATCAGCTACC TTACTGGTCC CACCAAGCGC AGCTCCCTGG GTCCCGGATT GCTGTGGTGG 
GACCTTGCTC GACAGACAGC GTCTGTGGCC CCAAAGGAAG ACACTGCCAC CCTGGAGGAG 
AGCCTGGTGA AGGGACCGGA AGACATCCCT GCTGTGACCA AGAAGCCCCC TGGCCTCAAG 
CCAGGCGCCG AGACCCACCC CCTGTATCTG GGGCACGATG TGGAGACCAA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_443215.2
CDS	85..1941
Translation	MEGAEAGARATFGAWDYGVFATMLLVSTGIGLWVGLARGGQRSADDFFTGGRQLAAVPVGLSLAASFMSAVQVLGVPAEAARYGLKFLWMCAGQLLNSLLTAFLFLPIFYRLGLTSTYQYLELRFSRAVRLCGTLQYLVATMLYTGIVIYAPALILNQVTGLDIWASLLSTGIICTLYTTVGGMKAVVWTDVFQVVVMLVGFWVILARGVILLGGPRNMLSLAQNHSRINLMDFDPDPRSRYTFWTFIVGGTLVWLSMYGVNQAQVQRYVACHTEGKAKLALLVNQLGLFLIVASAACCGIVMFVYYKDCDPLLTGRISAPDQYMPLLVLDIFEDLPGVPGLFLACAYSGTLSTASTSINAMAAVTVEDLIKPRMPGLAPRKLVFISKGLSFIYGSACLTVAALSSLLGGGVLQGSFTVMGVISGPLLGAFTLGMLLPACNTPGVLSGLAAGLAVSLWVAVGATLYPPGEQTMGVLPTSAAGCTNDSVLLGPPGATNASNGIPSSGMDTGRPALADTFYAISYLYYGALGTLTTMLCGALISYLTGPTKRSSLGPGLLWWDLARQTASVAPKEDTATLEESLVKGPEDIPAVTKKPPGLKPGAETHPLYLGHDVETNL

Target ORF information:

RefSeq Version	NM_052983.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 5 member 5 (Slc5a5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052983.2

ORF Insert Sequence:

ATGGAGGGTG CGGAGGCCGG GGCCCGGGCC ACCTTCGGCG CCTGGGACTA CGGCGTGTTC 
GCGACCATGC TGCTGGTGTC CACGGGCATC GGGCTATGGG TCGGCCTGGC CCGCGGTGGC 
CAACGCAGTG CCGACGACTT CTTTACCGGG GGCCGGCAGT TGGCAGCCGT TCCTGTGGGG 
CTGTCGCTGG CCGCCAGTTT CATGTCGGCT GTGCAGGTGC TCGGGGTCCC CGCCGAGGCA 
GCGCGCTACG GGCTCAAGTT CCTGTGGATG TGCGCGGGTC AGTTGCTCAA CTCGCTGCTC 
ACAGCGTTTC TCTTCTTGCC GATCTTCTAC CGCCTGGGCC TTACCAGCAC CTACCAGTAC 
CTAGAGCTGC GCTTCAGCCG AGCGGTCCGG CTCTGCGGGA CGCTGCAGTA CTTGGTGGCC 
ACGATGCTGT ATACAGGCAT CGTGATCTAC GCGCCTGCGC TCATCCTGAA CCAAGTGACC 
GGGTTGGACA TCTGGGCATC GCTCCTGTCC ACAGGAATCA TCTGCACCTT GTACACTACC 
GTGGGTGGTA TGAAGGCCGT GGTCTGGACA GATGTGTTCC AGGTTGTGGT AATGCTCGTT 
GGCTTCTGGG TGATCCTGGC CCGAGGCGTC ATTCTCCTGG GGGGTCCCCG GAACATGCTC 
AGCCTCGCTC AGAACCATTC CCGGATCAAC CTGATGGACT TTGACCCTGA TCCTCGGAGC 
CGGTACACCT TCTGGACTTT CATAGTGGGT GGCACACTGG TGTGGCTCTC CATGTACGGT 
GTGAACCAAG CCCAGGTACA GCGCTATGTG GCCTGCCACA CAGAGGGAAA GGCCAAACTG 
GCCCTGCTTG TCAACCAGCT GGGCCTCTTC CTGATTGTGG CCAGTGCAGC TTGCTGTGGC 
ATTGTCATGT TCGTCTACTA CAAGGACTGT GACCCCCTCC TCACAGGCCG TATCTCAGCC 
CCCGACCAGT ACATGCCGCT GCTTGTGTTG GACATTTTTG AGGATCTGCC CGGAGTCCCC 
GGGCTCTTCC TGGCCTGTGC CTACAGTGGC ACCCTCAGCA CTGCATCCAC CAGCATCAAC 
GCCATGGCAG CTGTGACTGT GGAAGACCTC ATCAAGCCGA GGATGCCTGG CCTGGCACCT 
CGGAAGTTGG TTTTCATCTC TAAAGGGCTC TCATTCATCT ACGGCTCTGC CTGCCTCACT 
GTGGCTGCTC TGTCCTCACT GCTGGGAGGT GGTGTCCTCC AGGGTTCCTT CACTGTGATG 
GGTGTCATCA GTGGGCCTCT ACTAGGCGCC TTCACGCTTG GGATGCTGCT CCCAGCCTGC 
AACACGCCAG GCGTTCTCTC CGGGTTGGCA GCAGGCTTGG CTGTATCCCT GTGGGTGGCC 
GTAGGGGCCA CACTGTATCC CCCTGGAGAG CAGACCATGG GGGTGCTGCC CACCTCGGCT 
GCAGGCTGCA CCAACGATTC GGTCCTCCTG GGCCCACCTG GAGCCACCAA CGCTTCCAAC 
GGGATCCCCA GTTCTGGAAT GGACACGGGC CGCCCTGCCC TCGCTGATAC CTTTTACGCC 
ATCTCCTATC TCTATTACGG GGCTCTGGGC ACGCTGACCA CCATGCTTTG CGGTGCTCTC 
ATCAGCTACC TTACTGGTCC CACCAAGCGC AGCTCCCTGG GTCCCGGATT GCTGTGGTGG 
GACCTTGCTC GACAGACAGC GTCTGTGGCC CCAAAGGAAG ACACTGCCAC CCTGGAGGAG 
AGCCTGGTGA AGGGACCGGA AGACATCCCT GCTGTGACCA AGAAGCCCCC TGGCCTCAAG 
CCAGGCGCCG AGACCCACCC CCTGTATCTG GGGCACGATG TGGAGACCAA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of thyroid sodium-iodide symporter in different stages of goiter: Possible involvement of reactive oxygen species.
Clinical and experimental pharmacology & physiology(2017 Nov)
Matos LPL,Penha RCC,Cardoso-Weide LC,Freitas ML,Silva DLSG,Ferreira ACF

Excess iodide downregulates Na(+)/I(-) symporter gene transcription through activation of PI3K/Akt pathway.
Molecular and cellular endocrinology42673-90(2016 May)
Serrano-Nascimento C,Nicola JP,Teixeira Sda S,Poyares LL,Lellis-Santos C,Bordin S,Masini-Repiso AM,Nunes MT

Selenium Increases Thyroid-Stimulating Hormone-Induced Sodium/Iodide Symporter Expression Through Thioredoxin/Apurinic/Apyrimidinic Endonuclease 1-Dependent Regulation of Paired Box 8 Binding Activity.
Antioxidants & redox signaling24(15)855-66(2016 May)
Leoni SG,Sastre-Perona A,De la Vieja A,Santisteban P

Beyond non-integer Hill coefficients: A novel approach to analyzing binding data, applied to Na+-driven transporters.
The Journal of general physiology145(6)555-63(2015 Jun)
Ravera S,Quick M,Nicola JP,Carrasco N,Amzel LM

Feasibility of lentiviral?mediated sodium iodide symporter gene delivery for the efficient monitoring of bone marrow?derived mesenchymal stem cell transplantation and survival.
International journal of molecular medicine34(6)1547-54(2014 Dec)
Shi S,Zhang M,Guo R,Miao Y,Zhang M,Hu J,Xi Y,Li B