Ltc4s cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ltc4s
Entrez Gene ID	114097
Full Name	leukotriene C4 synthase
General protein information	Preferred Names leukotriene C4 synthase Names leukotriene C4 synthase LTC4 synthase leukotriene-C(4) synthase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q22
Summary	conjugates leukotriene LTA4 with reduced glutathione (GSH) to form LTC4, mediates inflammatory responses in mast cells, eosinophils, basophils, monocytes-macrophages, and platelets [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008767655.2	XP_008765877.1	leukotriene C4 synthase isoform X1
NM_053639.2	NP_446091.2	leukotriene C4 synthase
XM_006246219.2	XP_006246281.1	leukotriene C4 synthase isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	mgst2	NP_001106565.1
Pan troglodytes (chimpanzee)	LTC4S	XP_001153488.2
	LTC4S	XP_001101985.1
Canis lupus familiaris (dog)	LTC4S	XP_849032.2
Bos taurus (cattle)	LTC4S	NP_001039563.2
Gallus gallus (chicken)	LOC770619	XP_001233958.3
Mus musculus (house mouse)	Ltc4s	NP_032547.1
Homo sapiens (human)	LTC4S	NP_665874.1
Rattus norvegicus (Norway rat)	Ltc4s	NP_446091.2

Gene Ontology
Bibliography

Related articles in PubMed

Leukotriene-C4 synthase, a critical enzyme in the activation of store-independent Orai1/Orai3 channels, is required for neointimal hyperplasia.
Zhang W, Zhang X, Gonz?lez-Cobos JC, Stolwijk JA, Matrougui K, Trebak M
The Journal of biological chemistry290(8)5015-27(2015 Feb)

[Expression of 5-lipoxygenase in hippocampal CA1 neuronal damage following global cerebral ischemia in rats].
Chen W, Li C, Zhao J, Zhang X, Han H, Wei E, Zhang L
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences42(1)61-6(2013 Jan)

Leukotriene synthases and the receptors induced by peripheral nerve injury in the spinal cord contribute to the generation of neuropathic pain.
Okubo M, Yamanaka H, Kobayashi K, Noguchi K
Glia58(5)599-610(2010 Apr)

Distinct parts of leukotriene C(4) synthase interact with 5-lipoxygenase and 5-lipoxygenase activating protein.
Strid T, Svartz J, Franck N, Hallin E, Ingelsson B, S?derstr?m M, Hammarstr?m S
Biochemical and biophysical research communications381(4)518-22(2009 Apr)

Enhanced expressions and activations of leukotriene C4 synthesis enzymes in D-galactosamine/lipopolysaccharide-induced rat fulminant hepatic failure model.
Ma KF, Yang HY, Chen Z, Qi LY, Zhu DY, Lou YJ
World journal of gastroenterology14(17)2748-56(2008 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LTC4S and Orai3 altered Akt signaling to promote VSMC migration and neointima formation.
Title: Leukotriene-C4 synthase, a critical enzyme in the activation of store-independent Orai1/Orai3 channels, is required for neointimal hyperplasia.

5-LOX is involved in global cerebral ischemic damage in rats
Title: [Expression of 5-lipoxygenase in hippocampal CA1 neuronal damage following global cerebral ischemia in rats].

Liver LTC4S was up-regulated in rat model of fulminant hepatic failure.
Title: Enhanced expressions and activations of leukotriene C4 synthesis enzymes in D-galactosamine/lipopolysaccharide-induced rat fulminant hepatic failure model.

In the myeloid cells, but not in the epithelial cells, we observed that the leukotriene C(4) synthase promoter activity was stimulated by 12-O-tetradecanoylphorbol-13-acetate and all-trans-retinoic acid.
Title: Leukotriene C4 synthase promoter driven expression of GFP reveals cell specificity.

hepatic ischemia reperfusion injury up-regulated the mRNA and protein expressions of LTC4S in hepatocytes and sinusoidal endothelial cells and enhanced the activities of the LTC4 synthesis enzymes
Title: Increased leukotriene c4 synthesis accompanied enhanced leukotriene c4 synthase expression and activities of ischemia-reperfusion-injured liver in rats.

The following Ltc4s gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ltc4s cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10159	NM_053639.2 Latest version!	Rattus norvegicus leukotriene C4 synthase (Ltc4s), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa33011	XM_008767655.2 Latest version!	Rattus norvegicus leukotriene C4 synthase (Ltc4s), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa33012	XM_006246219.2 Latest version!	Rattus norvegicus leukotriene C4 synthase (Ltc4s), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ltc4s ( NM_053639.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_446091.2 Rattus norvegicus leukotriene C4 synthase (Ltc4s), mRNA.

Price & Availability↑

CloneID	ORa10159
Clone ID Related Accession (Same CDS sequence)	NM_053639.2
Accession Version	NM_053639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	leukotriene C4 synthase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB048790.2. On Feb 20, 2002 this sequence version replaced NM_053639.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB048790.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906351 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGAAG AAACAGCTCT TCTGGCTACC GTCACCCTCG TGGGAGTTCT GCTGCAAGCC 
TACTTCTCCC TACAGGTGAT CTCTGCGCGA AGAACTTTCC ACGTGTCGCC GCCGCTCACC 
TCTGGCCCTC CCGAGTTCGA GCGCGTCTTC CGAGCCCAGG TAAATTGCAG CGAGTACTTT 
CCGCTGTTCC TCGCCACGCT CTGGGTCGCC GGCATCTTCT TCCACGAAGG CGCCGCAGCC 
CTGTGCGGGC TGTTCTACCT GTTCGCGCGG CTCCGCTACT TCCAGGGATA CGCGCGCTCC 
GCACAGCACA GGCTGGATCC CCTGTACGCG AGCGCGCGCG CGCTCTGGCT GCTAGTGGCA 
ATGGCTGCAC TGGGCTTGCT AGTCCACTTC CTCCCTGGCA CACTACGGGC TGCGCTCTTC 
AGATGGCTCC AGGTGCTCCT GCCGATGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_446091.2
CDS	26..478
Translation	MKEETALLATVTLVGVLLQAYFSLQVISARRTFHVSPPLTSGPPEFERVFRAQVNCSEYFPLFLATLWVAGIFFHEGAAALCGLFYLFARLRYFQGYARSAQHRLDPLYASARALWLLVAMAALGLLVHFLPGTLRAALFRWLQVLLPMA

Target ORF information:

RefSeq Version	NM_053639.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leukotriene C4 synthase (Ltc4s), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053639.2

ORF Insert Sequence:

ATGAAGGAAG AAACAGCTCT TCTGGCTACC GTCACCCTCG TGGGAGTTCT GCTGCAAGCC 
TACTTCTCCC TACAGGTGAT CTCTGCGCGA AGAACTTTCC ACGTGTCGCC GCCGCTCACC 
TCTGGCCCTC CCGAGTTCGA GCGCGTCTTC CGAGCCCAGG TAAATTGCAG CGAGTACTTT 
CCGCTGTTCC TCGCCACGCT CTGGGTCGCC GGCATCTTCT TCCACGAAGG CGCCGCAGCC 
CTGTGCGGGC TGTTCTACCT GTTCGCGCGG CTCCGCTACT TCCAGGGATA CGCGCGCTCC 
GCACAGCACA GGCTGGATCC CCTGTACGCG AGCGCGCGCG CGCTCTGGCT GCTAGTGGCA 
ATGGCTGCAC TGGGCTTGCT AGTCCACTTC CTCCCTGGCA CACTACGGGC TGCGCTCTTC 
AGATGGCTCC AGGTGCTCCT GCCGATGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ltc4s ( XM_008767655.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765877.1 Rattus norvegicus leukotriene C4 synthase (Ltc4s), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33011
Clone ID Related Accession (Same CDS sequence)	XM_008767655.2
Accession Version	XM_008767655.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	leukotriene C4 synthase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAAG AAACAGCTCT CCTGGCGACC GTCACCCTCC TGGGAGTTCT GCTGCAAGCC 
TACTTCTCCC TACAGGTGAT CTCTGCGCGA AGAACTTTCC ACGTGTCGCC GCCGCTCACC 
TCTGGCCCTC CCGAGTTCGA GCGCGTCTTC CGAGCCCAGG TAAATTGCAG CGAGTACTTT 
CCGCTGTTCC TCGCCACGCT CTGGGTCGCC GGCATCTTCT TCCACGAAGG CGCCGCAGCC 
CTGTGCGGAC TGTTCTACCT GTTCGCGCGG CTCCGCTACT TCCAGGGATA CGCGCGCTCC 
GCACAGCACA GGCTGGATCC CCTGTACGCG AGCGCGCGCG CGCTCTGGCT GCTAGTGGCA 
ATGGCTGCAC TGGGCTTGCT AGTCCACTTC CTCCCTGGCA CACTACGGGC TGCGCTCTTC 
AGATGGCTCC AGGTGCTCCT GCCGATGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765877.1
CDS	1520..1972
Translation	MKEETALLATVTLLGVLLQAYFSLQVISARRTFHVSPPLTSGPPEFERVFRAQVNCSEYFPLFLATLWVAGIFFHEGAAALCGLFYLFARLRYFQGYARSAQHRLDPLYASARALWLLVAMAALGLLVHFLPGTLRAALFRWLQVLLPMA

Target ORF information:

RefSeq Version	XM_008767655.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leukotriene C4 synthase (Ltc4s), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767655.2

ORF Insert Sequence:

ATGAAGGAAG AAACAGCTCT CCTGGCGACC GTCACCCTCC TGGGAGTTCT GCTGCAAGCC 
TACTTCTCCC TACAGGTGAT CTCTGCGCGA AGAACTTTCC ACGTGTCGCC GCCGCTCACC 
TCTGGCCCTC CCGAGTTCGA GCGCGTCTTC CGAGCCCAGG TAAATTGCAG CGAGTACTTT 
CCGCTGTTCC TCGCCACGCT CTGGGTCGCC GGCATCTTCT TCCACGAAGG CGCCGCAGCC 
CTGTGCGGAC TGTTCTACCT GTTCGCGCGG CTCCGCTACT TCCAGGGATA CGCGCGCTCC 
GCACAGCACA GGCTGGATCC CCTGTACGCG AGCGCGCGCG CGCTCTGGCT GCTAGTGGCA 
ATGGCTGCAC TGGGCTTGCT AGTCCACTTC CTCCCTGGCA CACTACGGGC TGCGCTCTTC 
AGATGGCTCC AGGTGCTCCT GCCGATGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ltc4s ( XM_006246219.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246281.1 Rattus norvegicus leukotriene C4 synthase (Ltc4s), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa33012
Clone ID Related Accession (Same CDS sequence)	XM_006246219.2
Accession Version	XM_006246219.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	leukotriene C4 synthase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006246219.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCCC CAAGGAGTGT GTACAGGGGA GCAGAGGAAA CTGTGCATGG GTCAACCGAG 
GAGGACCAGG TGATCTCTGC GCGAAGAACT TTCCACGTGT CGCCGCCGCT CACCTCTGGC 
CCTCCCGAGT TCGAGCGCGT CTTCCGAGCC CAGGTAAATT GCAGCGAGTA CTTTCCGCTG 
TTCCTCGCCA CGCTCTGGGT CGCCGGCATC TTCTTCCACG AAGGCGCCGC AGCCCTGTGC 
GGACTGTTCT ACCTGTTCGC GCGGCTCCGC TACTTCCAGG GATACGCGCG CTCCGCACAG 
CACAGGCTGG ATCCCCTGTA CGCGAGCGCG CGCGCGCTCT GGCTGCTAGT GGCAATGGCT 
GCACTGGGCT TGCTAGTCCA CTTCCTCCCT GGCACACTAC GGGCTGCGCT CTTCAGATGG 
CTCCAGGTGC TCCTGCCGAT GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246281.1
CDS	225..671
Translation	MKPPRSVYRGAEETVHGSTEEDQVISARRTFHVSPPLTSGPPEFERVFRAQVNCSEYFPLFLATLWVAGIFFHEGAAALCGLFYLFARLRYFQGYARSAQHRLDPLYASARALWLLVAMAALGLLVHFLPGTLRAALFRWLQVLLPMA

Target ORF information:

RefSeq Version	XM_006246219.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leukotriene C4 synthase (Ltc4s), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246219.2

ORF Insert Sequence:

ATGAAGCCCC CAAGGAGTGT GTACAGGGGA GCAGAGGAAA CTGTGCATGG GTCAACCGAG 
GAGGACCAGG TGATCTCTGC GCGAAGAACT TTCCACGTGT CGCCGCCGCT CACCTCTGGC 
CCTCCCGAGT TCGAGCGCGT CTTCCGAGCC CAGGTAAATT GCAGCGAGTA CTTTCCGCTG 
TTCCTCGCCA CGCTCTGGGT CGCCGGCATC TTCTTCCACG AAGGCGCCGC AGCCCTGTGC 
GGACTGTTCT ACCTGTTCGC GCGGCTCCGC TACTTCCAGG GATACGCGCG CTCCGCACAG 
CACAGGCTGG ATCCCCTGTA CGCGAGCGCG CGCGCGCTCT GGCTGCTAGT GGCAATGGCT 
GCACTGGGCT TGCTAGTCCA CTTCCTCCCT GGCACACTAC GGGCTGCGCT CTTCAGATGG 
CTCCAGGTGC TCCTGCCGAT GGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Leukotriene-C4 synthase, a critical enzyme in the activation of store-independent Orai1/Orai3 channels, is required for neointimal hyperplasia.
The Journal of biological chemistry290(8)5015-27(2015 Feb)
Zhang W,Zhang X,Gonz?lez-Cobos JC,Stolwijk JA,Matrougui K,Trebak M

[Expression of 5-lipoxygenase in hippocampal CA1 neuronal damage following global cerebral ischemia in rats].
Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences42(1)61-6(2013 Jan)
Chen W,Li C,Zhao J,Zhang X,Han H,Wei E,Zhang L

Leukotriene synthases and the receptors induced by peripheral nerve injury in the spinal cord contribute to the generation of neuropathic pain.
Glia58(5)599-610(2010 Apr)
Okubo M,Yamanaka H,Kobayashi K,Noguchi K

Distinct parts of leukotriene C(4) synthase interact with 5-lipoxygenase and 5-lipoxygenase activating protein.
Biochemical and biophysical research communications381(4)518-22(2009 Apr)
Strid T,Svartz J,Franck N,Hallin E,Ingelsson B,S?derstr?m M,Hammarstr?m S

Enhanced expressions and activations of leukotriene C4 synthesis enzymes in D-galactosamine/lipopolysaccharide-induced rat fulminant hepatic failure model.
World journal of gastroenterology14(17)2748-56(2008 May)
Ma KF,Yang HY,Chen Z,Qi LY,Zhu DY,Lou YJ