spast cDNA ORF clone, Pundamilia nyererei

Gene
Clones
gRNAs

Summary

Gene Symbol	spast
Entrez Gene ID	102200509
Full Name	spastin
Gene Type	protein-coding
Organism	Pundamilia nyererei

mRNA and Protein(s)

mRNA	Protein	Name
XM_005742464.1	XP_005742521.1	spastin isoform X1
XM_005742465.1	XP_005742522.1	spastin isoform X2

Pathways from BioSystems
Gene Ontology

The following spast gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spast cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPu72178	XM_005742464.1 Latest version!	Pundamilia nyererei spastin (spast), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OPu72179	XM_005742465.1 Latest version!	Pundamilia nyererei spastin (spast), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

spast ( XM_005742464.1 ) cDNA ORF clone, Pundamilia nyererei -> XP_005742521.1 Pundamilia nyererei spastin (spast), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPu72178
Clone ID Related Accession (Same CDS sequence)	XM_005742464.1
Accession Version	XM_005742464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1434bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-30
Organism	Pundamilia nyererei
Product	spastin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005187521.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pundamilia nyererei Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCA AAACAAACGC AAGTAAGCCC AAAGACAGCG CTGAAGTTAT AAAAAACATC 
CACAAGCAGG CGTTTGAGTA TATATCCAAG GCACTGAAGA TCGACGAGGA CGACACAGGT 
GAGAAGAAGG AGGCTGTGCA GTGGTACAAG AAAGGGATTT CTGAGCTTGA AAGGGGTATT 
GCAATAGAGC TCACTGGGCA AGCAGGAGAG CAGTATGACC GAGCAAAGAG ACTCCAGGAT 
AAAATGGTCA CCAACCTCAC CATGGCAAAA GACAGGCTTA CCCTTTTAGA GACGACACTA 
GCCTCTAAAA GGAGAAATGA CCCCCAAGGG ACATCACGTC ATGCTCTTCC ACAGCCAAAG 
TCAGTTCCTA AAAGTCAACC CGCAGGTGTT TCCACCACCA TCAGACCCTC CACTCCTGTA 
AGACCTTCAT TAAGACTAAC TGATGCAAAG ACAACTGCTC GGGTGGGAAA AACTCAGAAT 
GGAAGACCGG CAGCTGTGAA ACAACCCCCG AAGAGGGATA TGAAAAACTT CAAGAATGTG 
GACAGTAAAC TGGCCAACTT AATTATGAAT GAAATTGTAG ACAGTGGAGC GACTGTGTCA 
TTTGATGACA TCGCAGGGCA GGATCTGGCA AAGCAAGCAC TTCAAGAAAT TGTCATCCTT 
CCTGCCTTAA GACCTGAGCT CTTTACTGGT CTGAGAGCTC CTGCACGTGG CTTGCTTTTA 
TTTGGCCCAC CAGGAAATGG GAAGACCATG CTCGCCAAAG CAGTTGCTGC AGAGTCTAAT 
GCAACATTCT TCAACATCAG TGCTGCTAGT TTGACCTCCA AATATGTGGG AGAAGGCGAG 
AAGCTTGTAC GAGCACTCTT TGCGGTTGCA AGAGAGTTAC AGCCCTCAGT AATCTTCATC 
GATGAAGTGG ACAGCTTGCT CTGTGAAAGG AGGGAGGGGG AGCACGACGC CTCTCGCCGT 
TTAAAAACTG AGTTCCTCAT TGAGTTTGAC GGGGTGCAGT CAGGAAAGGA TGACAGGGTA 
CTTGTAATGG GAGCAACTAA CAGGCCCCAG GAGCTCGATG AAGCAATATT AAGGCGCTTT 
GCAAAAAGAG TATTTGTGAC CTTACCCGAT GAGAAGACAA GATTTACGCT GCTGAAAAAT 
CTTTTGGGAA AGCACGGGAG TCCACTGAGC CAAAACGAAC TGTCCTGTCT TGCAAAAGTG 
ACTGCAGGAT ATTCAGGGAG CGATTTGACA GCATTAGCCA GAGATGCTGC ACTTGGCCCA 
ATACGAGAGT TGGGACCAGA CCAAGTCCGT AATATGGCTG CTGCTGAGGT GCGCAACATT 
AAGAAGAAAG ACTTTGAGGA TTCTCTGAAG CGAATCAAAC CAACTGTTAG TCCAGCAACT 
CTTACTATGT ACACAAAATG GAACAAAGAT TTTGGTGATA CATCAGCAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005742521.1
CDS	284..1717
Translation	MSTKTNASKPKDSAEVIKNIHKQAFEYISKALKIDEDDTGEKKEAVQWYKKGISELERGIAIELTGQAGEQYDRAKRLQDKMVTNLTMAKDRLTLLETTLASKRRNDPQGTSRHALPQPKSVPKSQPAGVSTTIRPSTPVRPSLRLTDAKTTARVGKTQNGRPAAVKQPPKRDMKNFKNVDSKLANLIMNEIVDSGATVSFDDIAGQDLAKQALQEIVILPALRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSVIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSGKDDRVLVMGATNRPQELDEAILRRFAKRVFVTLPDEKTRFTLLKNLLGKHGSPLSQNELSCLAKVTAGYSGSDLTALARDAALGPIRELGPDQVRNMAAAEVRNIKKKDFEDSLKRIKPTVSPATLTMYTKWNKDFGDTSAI

Target ORF information:

RefSeq Version	XM_005742464.1
Organism	Pundamilia nyererei
Definition	Pundamilia nyererei spastin (spast), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005742464.1

ORF Insert Sequence:

ATGTCGACCA AAACAAACGC AAGTAAGCCC AAAGACAGCG CTGAAGTTAT AAAAAACATC 
CACAAGCAGG CGTTTGAGTA TATATCCAAG GCACTGAAGA TCGACGAGGA CGACACAGGT 
GAGAAGAAGG AGGCTGTGCA GTGGTACAAG AAAGGGATTT CTGAGCTTGA AAGGGGTATT 
GCAATAGAGC TCACTGGGCA AGCAGGAGAG CAGTATGACC GAGCAAAGAG ACTCCAGGAT 
AAAATGGTCA CCAACCTCAC CATGGCAAAA GACAGGCTTA CCCTTTTAGA GACGACACTA 
GCCTCTAAAA GGAGAAATGA CCCCCAAGGG ACATCACGTC ATGCTCTTCC ACAGCCAAAG 
TCAGTTCCTA AAAGTCAACC CGCAGGTGTT TCCACCACCA TCAGACCCTC CACTCCTGTA 
AGACCTTCAT TAAGACTAAC TGATGCAAAG ACAACTGCTC GGGTGGGAAA AACTCAGAAT 
GGAAGACCGG CAGCTGTGAA ACAACCCCCG AAGAGGGATA TGAAAAACTT CAAGAATGTG 
GACAGTAAAC TGGCCAACTT AATTATGAAT GAAATTGTAG ACAGTGGAGC GACTGTGTCA 
TTTGATGACA TCGCAGGGCA GGATCTGGCA AAGCAAGCAC TTCAAGAAAT TGTCATCCTT 
CCTGCCTTAA GACCTGAGCT CTTTACTGGT CTGAGAGCTC CTGCACGTGG CTTGCTTTTA 
TTTGGCCCAC CAGGAAATGG GAAGACCATG CTCGCCAAAG CAGTTGCTGC AGAGTCTAAT 
GCAACATTCT TCAACATCAG TGCTGCTAGT TTGACCTCCA AATATGTGGG AGAAGGCGAG 
AAGCTTGTAC GAGCACTCTT TGCGGTTGCA AGAGAGTTAC AGCCCTCAGT AATCTTCATC 
GATGAAGTGG ACAGCTTGCT CTGTGAAAGG AGGGAGGGGG AGCACGACGC CTCTCGCCGT 
TTAAAAACTG AGTTCCTCAT TGAGTTTGAC GGGGTGCAGT CAGGAAAGGA TGACAGGGTA 
CTTGTAATGG GAGCAACTAA CAGGCCCCAG GAGCTCGATG AAGCAATATT AAGGCGCTTT 
GCAAAAAGAG TATTTGTGAC CTTACCCGAT GAGAAGACAA GATTTACGCT GCTGAAAAAT 
CTTTTGGGAA AGCACGGGAG TCCACTGAGC CAAAACGAAC TGTCCTGTCT TGCAAAAGTG 
ACTGCAGGAT ATTCAGGGAG CGATTTGACA GCATTAGCCA GAGATGCTGC ACTTGGCCCA 
ATACGAGAGT TGGGACCAGA CCAAGTCCGT AATATGGCTG CTGCTGAGGT GCGCAACATT 
AAGAAGAAAG ACTTTGAGGA TTCTCTGAAG CGAATCAAAC CAACTGTTAG TCCAGCAACT 
CTTACTATGT ACACAAAATG GAACAAAGAT TTTGGTGATA CATCAGCAAT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

spast ( XM_005742465.1 ) cDNA ORF clone, Pundamilia nyererei -> XP_005742522.1 Pundamilia nyererei spastin (spast), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OPu72179
Clone ID Related Accession (Same CDS sequence)	XM_005742465.1
Accession Version	XM_005742465.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1431bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-30
Organism	Pundamilia nyererei
Product	spastin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005187521.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pundamilia nyererei Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCA AAACAAACGC AAGTAAGCCC AAAGACAGCG CTGAAGTTAT AAAAAACATC 
CACAAGCAGG CGTTTGAGTA TATATCCAAG GCACTGAAGA TCGACGAGGA CGACACAGGT 
GAGAAGAAGG AGGCTGTGCA GTGGTACAAG AAAGGGATTT CTGAGCTTGA AAGGGGTATT 
GCAATAGAGC TCACTGGGCA AGGAGAGCAG TATGACCGAG CAAAGAGACT CCAGGATAAA 
ATGGTCACCA ACCTCACCAT GGCAAAAGAC AGGCTTACCC TTTTAGAGAC GACACTAGCC 
TCTAAAAGGA GAAATGACCC CCAAGGGACA TCACGTCATG CTCTTCCACA GCCAAAGTCA 
GTTCCTAAAA GTCAACCCGC AGGTGTTTCC ACCACCATCA GACCCTCCAC TCCTGTAAGA 
CCTTCATTAA GACTAACTGA TGCAAAGACA ACTGCTCGGG TGGGAAAAAC TCAGAATGGA 
AGACCGGCAG CTGTGAAACA ACCCCCGAAG AGGGATATGA AAAACTTCAA GAATGTGGAC 
AGTAAACTGG CCAACTTAAT TATGAATGAA ATTGTAGACA GTGGAGCGAC TGTGTCATTT 
GATGACATCG CAGGGCAGGA TCTGGCAAAG CAAGCACTTC AAGAAATTGT CATCCTTCCT 
GCCTTAAGAC CTGAGCTCTT TACTGGTCTG AGAGCTCCTG CACGTGGCTT GCTTTTATTT 
GGCCCACCAG GAAATGGGAA GACCATGCTC GCCAAAGCAG TTGCTGCAGA GTCTAATGCA 
ACATTCTTCA ACATCAGTGC TGCTAGTTTG ACCTCCAAAT ATGTGGGAGA AGGCGAGAAG 
CTTGTACGAG CACTCTTTGC GGTTGCAAGA GAGTTACAGC CCTCAGTAAT CTTCATCGAT 
GAAGTGGACA GCTTGCTCTG TGAAAGGAGG GAGGGGGAGC ACGACGCCTC TCGCCGTTTA 
AAAACTGAGT TCCTCATTGA GTTTGACGGG GTGCAGTCAG GAAAGGATGA CAGGGTACTT 
GTAATGGGAG CAACTAACAG GCCCCAGGAG CTCGATGAAG CAATATTAAG GCGCTTTGCA 
AAAAGAGTAT TTGTGACCTT ACCCGATGAG AAGACAAGAT TTACGCTGCT GAAAAATCTT 
TTGGGAAAGC ACGGGAGTCC ACTGAGCCAA AACGAACTGT CCTGTCTTGC AAAAGTGACT 
GCAGGATATT CAGGGAGCGA TTTGACAGCA TTAGCCAGAG ATGCTGCACT TGGCCCAATA 
CGAGAGTTGG GACCAGACCA AGTCCGTAAT ATGGCTGCTG CTGAGGTGCG CAACATTAAG 
AAGAAAGACT TTGAGGATTC TCTGAAGCGA ATCAAACCAA CTGTTAGTCC AGCAACTCTT 
ACTATGTACA CAAAATGGAA CAAAGATTTT GGTGATACAT CAGCAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005742522.1
CDS	284..1714
Translation	MSTKTNASKPKDSAEVIKNIHKQAFEYISKALKIDEDDTGEKKEAVQWYKKGISELERGIAIELTGQGEQYDRAKRLQDKMVTNLTMAKDRLTLLETTLASKRRNDPQGTSRHALPQPKSVPKSQPAGVSTTIRPSTPVRPSLRLTDAKTTARVGKTQNGRPAAVKQPPKRDMKNFKNVDSKLANLIMNEIVDSGATVSFDDIAGQDLAKQALQEIVILPALRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSVIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSGKDDRVLVMGATNRPQELDEAILRRFAKRVFVTLPDEKTRFTLLKNLLGKHGSPLSQNELSCLAKVTAGYSGSDLTALARDAALGPIRELGPDQVRNMAAAEVRNIKKKDFEDSLKRIKPTVSPATLTMYTKWNKDFGDTSAI

Target ORF information:

RefSeq Version	XM_005742465.1
Organism	Pundamilia nyererei
Definition	Pundamilia nyererei spastin (spast), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005742465.1

ORF Insert Sequence:

ATGTCGACCA AAACAAACGC AAGTAAGCCC AAAGACAGCG CTGAAGTTAT AAAAAACATC 
CACAAGCAGG CGTTTGAGTA TATATCCAAG GCACTGAAGA TCGACGAGGA CGACACAGGT 
GAGAAGAAGG AGGCTGTGCA GTGGTACAAG AAAGGGATTT CTGAGCTTGA AAGGGGTATT 
GCAATAGAGC TCACTGGGCA AGGAGAGCAG TATGACCGAG CAAAGAGACT CCAGGATAAA 
ATGGTCACCA ACCTCACCAT GGCAAAAGAC AGGCTTACCC TTTTAGAGAC GACACTAGCC 
TCTAAAAGGA GAAATGACCC CCAAGGGACA TCACGTCATG CTCTTCCACA GCCAAAGTCA 
GTTCCTAAAA GTCAACCCGC AGGTGTTTCC ACCACCATCA GACCCTCCAC TCCTGTAAGA 
CCTTCATTAA GACTAACTGA TGCAAAGACA ACTGCTCGGG TGGGAAAAAC TCAGAATGGA 
AGACCGGCAG CTGTGAAACA ACCCCCGAAG AGGGATATGA AAAACTTCAA GAATGTGGAC 
AGTAAACTGG CCAACTTAAT TATGAATGAA ATTGTAGACA GTGGAGCGAC TGTGTCATTT 
GATGACATCG CAGGGCAGGA TCTGGCAAAG CAAGCACTTC AAGAAATTGT CATCCTTCCT 
GCCTTAAGAC CTGAGCTCTT TACTGGTCTG AGAGCTCCTG CACGTGGCTT GCTTTTATTT 
GGCCCACCAG GAAATGGGAA GACCATGCTC GCCAAAGCAG TTGCTGCAGA GTCTAATGCA 
ACATTCTTCA ACATCAGTGC TGCTAGTTTG ACCTCCAAAT ATGTGGGAGA AGGCGAGAAG 
CTTGTACGAG CACTCTTTGC GGTTGCAAGA GAGTTACAGC CCTCAGTAAT CTTCATCGAT 
GAAGTGGACA GCTTGCTCTG TGAAAGGAGG GAGGGGGAGC ACGACGCCTC TCGCCGTTTA 
AAAACTGAGT TCCTCATTGA GTTTGACGGG GTGCAGTCAG GAAAGGATGA CAGGGTACTT 
GTAATGGGAG CAACTAACAG GCCCCAGGAG CTCGATGAAG CAATATTAAG GCGCTTTGCA 
AAAAGAGTAT TTGTGACCTT ACCCGATGAG AAGACAAGAT TTACGCTGCT GAAAAATCTT 
TTGGGAAAGC ACGGGAGTCC ACTGAGCCAA AACGAACTGT CCTGTCTTGC AAAAGTGACT 
GCAGGATATT CAGGGAGCGA TTTGACAGCA TTAGCCAGAG ATGCTGCACT TGGCCCAATA 
CGAGAGTTGG GACCAGACCA AGTCCGTAAT ATGGCTGCTG CTGAGGTGCG CAACATTAAG 
AAGAAAGACT TTGAGGATTC TCTGAAGCGA ATCAAACCAA CTGTTAGTCC AGCAACTCTT 
ACTATGTACA CAAAATGGAA CAAAGATTTT GGTGATACAT CAGCAATTTA A