TNFRSF1A cDNA ORF clone,

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gRNAs

The following TNFRSF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNFRSF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPu173679	XM_025930934.1 Latest version!	Puma concolor TNF receptor superfamily member 1A (TNFRSF1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNFRSF1A ( XM_025930934.1 ) cDNA ORF clone, -> XP_025786719.1 Puma concolor TNF receptor superfamily member 1A (TNFRSF1A), mRNA.

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CloneID	OPu173679
Clone ID Related Accession (Same CDS sequence)	XM_025930934.1
Accession Version	XM_025930934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1518bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-25
Organism	Puma concolor(puma)
Product	tumor necrosis factor receptor superfamily member 1A
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_020339853.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Puma concolor Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 13% of CDS bases ##RefSeq-Attributes-END##

ATGGGAACCT TGGGGAAAGA GGGGGAGATG GTGAAGGGAG TGGAGAGCCC AGCCTGGGCC 
TTGCCCCTCC CCAGGCCCAG CCCCTCTCCT CTGGCAGACT TCATTATGGA TGATGGCAAT 
CAGTTCCTCT ACCCCCACCA CCCACCCCTA CTAACCATGC TGGAGTTTCC TTATCTCCAT 
GCCCTATCTG ATCTCCTCTG TCTGATTCTT CCCCAGGTAC TTCTGGCTCT GTTGGTGGAA 
ATATACCCAT TAAGGGTTAT TGGACTGGTC CCTCACCTCA GGGACCGGGA GAAGAGAGCT 
ATTCCATGTC CCCAAGGAAA ATATATTCAC CCTCAAGATA ATTCCATTTG CTGTACGAAG 
TGCCACAAAG GGACCTACCT GTACAATGAC TGTGCGGGCC CAGGGCTGGA CACGGACTGC 
AGAGAATGTG AAAATGGCAC ATTTACTGCT TCAGAGAACT ACCTCAGACA GTGCCTTAGC 
TGCTCCAAAT GCCGAAAAGA AATGTACCAG GTGGAGATAT CTCCTTGCAC AGTGTACCGG 
GACACCGTGT GTGGCTGCAG GAAGAACCAG TACCGGTATT ACTGGAGTGA AACCCATTTC 
CAGTGCCTAA ACTGCAGCCT CTGCCTCAAC GGCACGGTGC AGATCTCCTG CAAGGAGACA 
CAGAATACTG TATGCACCTG CCACGCGGGG TTCTTTCTAA GAGGGAATGA ATGCGTCTCT 
TGTGTTAACT GTAAGAAAAA CACAGAGTGT ACGAAGTTGT GCGTACCCAT CGTGGAAACT 
GTTAAGGACC CTCAGGACCC AGGCACCACA GTGCTGCTGC CTCTCGTGAT CTTCTTCGGC 
ATTTGCGTTT TATCCTTCTC CATTGGTTTA ATGTGCCGCT ACCAACGGCG GAAGTCCAAG 
CTCTTCTCCA TTGTTTGTGG GAAATCAACA CCTACAAAAG AGGGAGAGCC TCAGCCCCTG 
GCCACAGGTC CAGGCTTCAG TCCCATCCCC AGCCCCACCT TTACCCCCAG CCCCACCTTC 
ACTCCCAGCC CCACCTTCAC TCCTAGCGAC TGGGCTAATT TGAGGGCTGC ATCCGTCTCC 
AGAGAGATGG CTCCACCCTA CCAGGGGGCT GGCCCCATCC TCTCCGCGGC TCCAGCCTCC 
AGCCCCATCT CCACCCCAGT TCAGAAATGG GAGGACAGCA CCCACACTCA ACGCCCAGAA 
GTGGATCCCG CCGACCCGGC GACGCTGTAC GCGGTGGTGG ACGGCGTGCC CCCGTCGCGC 
TGGAAGGAGT TCGTGCGGCG GCTGGGGCTG AGCGAGCACG AGATCGAGCG GCTGGAGCTG 
CAGAACGGGC GCTGCCTGCG CGAGGCGCAC TACAGCATGC TGGCGGCCTG GCGGCGACGC 
ACGCCACGAC GCGAAGCCAC GCTGGAGCTG CTGGGCCGCG TGCTCCGCGA CATGGACCTG 
CTCGGCTGCC TGGAGGACAT CGAGGAGGCG CTGTGCGCCC CGGCCTCCCT GTCGCCCCCG 
CCCCGCCTCC TCCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025786719.1
CDS	1..1518
Translation	MGTLGKEGEMVKGVESPAWALPLPRPSPSPLADFIMDDGNQFLYPHHPPLLTMLEFPYLHALSDLLCLILPQVLLALLVEIYPLRVIGLVPHLRDREKRAIPCPQGKYIHPQDNSICCTKCHKGTYLYNDCAGPGLDTDCRECENGTFTASENYLRQCLSCSKCRKEMYQVEISPCTVYRDTVCGCRKNQYRYYWSETHFQCLNCSLCLNGTVQISCKETQNTVCTCHAGFFLRGNECVSCVNCKKNTECTKLCVPIVETVKDPQDPGTTVLLPLVIFFGICVLSFSIGLMCRYQRRKSKLFSIVCGKSTPTKEGEPQPLATGPGFSPIPSPTFTPSPTFTPSPTFTPSDWANLRAASVSREMAPPYQGAGPILSAAPASSPISTPVQKWEDSTHTQRPEVDPADPATLYAVVDGVPPSRWKEFVRRLGLSEHEIERLELQNGRCLREAHYSMLAAWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCAPASLSPPPRLLR

Target ORF information:

RefSeq Version	XM_025930934.1
Organism	Puma concolor(puma)
Definition	Puma concolor TNF receptor superfamily member 1A (TNFRSF1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025930934.1

ORF Insert Sequence:

ATGGGAACCT TGGGGAAAGA GGGGGAGATG GTGAAGGGAG TGGAGAGCCC AGCCTGGGCC 
TTGCCCCTCC CCAGGCCCAG CCCCTCTCCT CTGGCAGACT TCATTATGGA TGATGGCAAT 
CAGTTCCTCT ACCCCCACCA CCCACCCCTA CTAACCATGC TGGAGTTTCC TTATCTCCAT 
GCCCTATCTG ATCTCCTCTG TCTGATTCTT CCCCAGGTAC TTCTGGCTCT GTTGGTGGAA 
ATATACCCAT TAAGGGTTAT TGGACTGGTC CCTCACCTCA GGGACCGGGA GAAGAGAGCT 
ATTCCATGTC CCCAAGGAAA ATATATTCAC CCTCAAGATA ATTCCATTTG CTGTACGAAG 
TGCCACAAAG GGACCTACCT GTACAATGAC TGTGCGGGCC CAGGGCTGGA CACGGACTGC 
AGAGAATGTG AAAATGGCAC ATTTACTGCT TCAGAGAACT ACCTCAGACA GTGCCTTAGC 
TGCTCCAAAT GCCGAAAAGA AATGTACCAG GTGGAGATAT CTCCTTGCAC AGTGTACCGG 
GACACCGTGT GTGGCTGCAG GAAGAACCAG TACCGGTATT ACTGGAGTGA AACCCATTTC 
CAGTGCCTAA ACTGCAGCCT CTGCCTCAAC GGCACGGTGC AGATCTCCTG CAAGGAGACA 
CAGAATACTG TATGCACCTG CCACGCGGGG TTCTTTCTAA GAGGGAATGA ATGCGTCTCT 
TGTGTTAACT GTAAGAAAAA CACAGAGTGT ACGAAGTTGT GCGTACCCAT CGTGGAAACT 
GTTAAGGACC CTCAGGACCC AGGCACCACA GTGCTGCTGC CTCTCGTGAT CTTCTTCGGC 
ATTTGCGTTT TATCCTTCTC CATTGGTTTA ATGTGCCGCT ACCAACGGCG GAAGTCCAAG 
CTCTTCTCCA TTGTTTGTGG GAAATCAACA CCTACAAAAG AGGGAGAGCC TCAGCCCCTG 
GCCACAGGTC CAGGCTTCAG TCCCATCCCC AGCCCCACCT TTACCCCCAG CCCCACCTTC 
ACTCCCAGCC CCACCTTCAC TCCTAGCGAC TGGGCTAATT TGAGGGCTGC ATCCGTCTCC 
AGAGAGATGG CTCCACCCTA CCAGGGGGCT GGCCCCATCC TCTCCGCGGC TCCAGCCTCC 
AGCCCCATCT CCACCCCAGT TCAGAAATGG GAGGACAGCA CCCACACTCA ACGCCCAGAA 
GTGGATCCCG CCGACCCGGC GACGCTGTAC GCGGTGGTGG ACGGCGTGCC CCCGTCGCGC 
TGGAAGGAGT TCGTGCGGCG GCTGGGGCTG AGCGAGCACG AGATCGAGCG GCTGGAGCTG 
CAGAACGGGC GCTGCCTGCG CGAGGCGCAC TACAGCATGC TGGCGGCCTG GCGGCGACGC 
ACGCCACGAC GCGAAGCCAC GCTGGAGCTG CTGGGCCGCG TGCTCCGCGA CATGGACCTG 
CTCGGCTGCC TGGAGGACAT CGAGGAGGCG CTGTGCGCCC CGGCCTCCCT GTCGCCCCCG 
CCCCGCCTCC TCCGGTGA