BUB1B cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	BUB1B
Entrez Gene ID	102896980
Full Name	BUB1 mitotic checkpoint serine/threonine kinase B
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006920498.2	XP_006920560.1	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
XM_006920498.3	XP_006920560.1	mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Pathways from BioSystems
Gene Ontology

The following BUB1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPt24876	XM_006920498.3 Latest version!	Pteropus alecto BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
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OPt24876	XM_006920498.2	Pteropus alecto BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUB1B ( XM_006920498.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006920560.1 Pteropus alecto BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

Price & Availability↑

CloneID	OPt24876
Clone ID Related Accession (Same CDS sequence)	XM_006920498.2 , XM_006920498.3
Accession Version	XM_006920498.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006442109.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 10, 2016 this sequence version replaced XM_006920498.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAA TGAAGAAGGA AGAGGGTGGA CCGAGTGAAG CCATGTCTCT GGAGGGAGAT 
GAATGGGAGC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AGGGGCGGAG CATGTCCACA 
CTTCAGGGAA TATTGGCACA GCAAGAATCT GCCCGTAACA CTACTCTACA GCAGCAGAAA 
CGGGCATTTG AATCTGAAAT TCGATTTTAC ACTGGAAATG ACCCTCTGGA TGTTTGGGAT 
AGGTATATTA ACTGGACAGA GCAGAACTAT CCTCAGGGGG GCAAGGAGAG TAATATGTCA 
ACATTATTAG AAAGAGCTGT AGAAGCACTA CAAGGAGAAA AAAGATATTA TAATGATCCT 
CGATTTCTCA GTCTCTGGCT TAAATTGGGA CATTTATGCA ATGAGCCTTT GGATATGTAC 
AGTTATTTAC ACAACCAAGG GATTGGCATT TCACTTGCTC AGTTCTATAT TTCATGGGCT 
GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCAGACA TGATATTTCA GGATGGAATT 
CAACAGAAGG CTGAACCACT GGAAAGACTG CAATCCCAGC ACCGACAATT CCAAGCTCGA 
GTGACTCGGC AAACTCTGAT GGCACTTGAG AAAGAAGAAG AGGAAGTTTT TGGAGCCTCT 
GTACCACAGC GAAGCACACT GGCTGAACTA AAAAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCAGCC GTATAGGAGG TGCTCTCAAG GCTCCAAACC AGAACCGAGG ACTCCAAAAT 
CCATTTCCTC AACCGATGCA AAGTAATTCT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA TAGCAGAGCT GTCTACGCCT ACAGTCCAGC CATGGATTGC ACCCCCTGTG 
TCCAGGGCCA AAGAAAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GCCTTTGGAA 
CACAGACCTC GTGGCAATAC AGCTTCTGTG ACAGCTGTAC CCTCTATGCT TCCCAGTTTT 
ACTCCGTATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCC 
AGTATAAACC ACGTTCTAAG CACAAGAAAG CCTGGAAAGG AAGAAGGGGA TCTCCTGCAA 
AGGGTTCAAA GCCATCAGCA AGATTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAAATTT ATGCAGGAGT TGGGGAGTTC TCCTTTGAAG AGATCCGGGC TGAAGTTTTC 
CGAAAGAAAT TAAAAGAGCA AAGGGAAGCT GAGCTACTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAAATTGA AGAGATGGAG AAGAAGCTGA AAGAAATTCA AACCACTCAG 
CATGAAAGAA CGGGTGATCA GCAAGAAGAG ATGTCTACAA AGGAGACAGT TGAACTGCAA 
ATTGCTTCAG TGTCTCAGGA AATCCCAGGA ATGACTCTAT CCAATTCTGT TTGTCCAATA 
AACTCTTGTG CCAGGGAAAC TTCACTTGCA GAAAACATTT GGCAAGAACA ACCTCATTCT 
AAAGATCCCA GTATACCTTT CTCCATTTTT GATGAGTTTC TTCTTTCAGA AAAAAGGAAT 
ATCAGTCCCT CTGCAGATCC CCCACGGGTT TTAGTCCAAC GAAGACCCCT TGCAGTTCTC 
AAAACTTCAG AAAACATCAC CTCAAATGAG GATGTGTCTC CAGATGTTTG TGATGAATTC 
ACAGGAATTG AGCCCTTGAG TGAAGATGCT ATTATCACAG GTTTCAGGAA TATAACTATT 
TGTCCTAACC CAGAAGACAC TTGTGACTTT GCTAGCGCAG CTCGTTTTGT ATCCACTCCT 
TTTCATGAGA TAATATCCTT GAAGGATCTC CCTTCTGACT CTGAGAGACT TTTGCAGGAA 
GAGGATCTAG ATGTGAAGAC CTCTGAGGAC CAACAGACTA CTTGTGGCAC TATCTACAAT 
CCAACTCTCA GCATCAAGAA GCTGAGCCCA ATTATTGAAG ATAGCCGCGA GGCCACACAC 
TCCTCTGGGT TCTCTGGATC TTCTGCCTCA GTTATAAGCA CCTGCTCCCT CAAATGCCTT 
CAAATTCCTG AGAAACTGGA GCTTACTAAT GAAACTGCAG AAAATCCTGT TCAGTCACCC 
TGGTGTTCAC AGTATCGTAG ACAACTACTA AAATCCCTAC CAGAGCTGAG TGCCTCTGTA 
GAGATTTTTG TAGAAGATAG ACCAATGCCT AAGTTGGAAA CAGAGAAGGA AATTGAATTA 
GGTAATGAGG ATTATTGCAT TAAACAAGAA TACCTAGTAT GTGAAGATTA CAAATTATTC 
TGGGTGACAC CAAGAAACTC TACAGAATTA ACCATAATAA AGGTATCTTC TCAAACTGTC 
CCATGGGACT TTTATATCAA CCTCAAGTTA AAGGAACGTT TAAATGAGGA GTGTGATCAT 
TTGTGCAGCT ATTATCAATA CCAAGATGGT TGTATTGTTT GGCACCAGTA TATAAACTGC 
TTCACCCTTC AGGATCTTTT TCAGCACAGT GAATTTATTA CCCATGAAAT AACAGTGTTG 
ATAGTTTATA ACCTTTTGAC AATAGTGGAG AAACTACACA AAGCGGAAAT AGTCCATGGT 
GACTTGAGTC CAAGGAGTCT GATTCTTAGA AACAGAATCC ACGATCCCTA TGATTGTAAT 
AAGAACAATC AAGCTTTGAA CATAGTGGAC TTTTCCTACA GTGTCGACCT CAGGGCTCAG 
CTAGACATTT TTGCCCTCAG TGGCTTTCGG ACTGTACAGA TCCTGGAAGG ACAAAAGATC 
CTGGCTAACT GTTCTTCTCC CTACCAGGTA GATCTGTTTG GTATAGCAGA TTTAGCACAT 
TTACTATTGT TCAAGGAACA TCTACAGGTC TTCTGGGATG GGTCCCTCTG GAAACTTAGC 
CAAAATATTT CTGAGCTAAA AGATGGTGAA TTGTGGAATA AATTCTTTGT GCGGATTCTG 
AATGCCAGTG ATGAGTCCAC AGTGTCTGTT CTGAGGGAAC TTGCAGCAGA AATCAATTGG 
GTGTTTGATA CCACATTTCA AAGTCACCTG AACAAAGCTT TATGGAAGGT AGGGAAGTTA 
ATCGGTCCTG GGGCTTTGCT CTTCCAGCAA GATAGGCAGT CAAGTCTCTC ACAGAATCCT 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006920560.1
CDS	16..3201
Translation	MAAMKKEEGGPSEAMSLEGDEWELSKENVQPLRQGRSMSTLQGILAQQESARNTTLQQQKRAFESEIRFYTGNDPLDVWDRYINWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYNDPRFLSLWLKLGHLCNEPLDMYSYLHNQGIGISLAQFYISWAEEYEARENFKKADMIFQDGIQQKAEPLERLQSQHRQFQARVTRQTLMALEKEEEEVFGASVPQRSTLAELKSKGKKTARAPISRIGGALKAPNQNRGLQNPFPQPMQSNSRITVFDENADEASIAELSTPTVQPWIAPPVSRAKENELQAGPWNTGRPLEHRPRGNTASVTAVPSMLPSFTPYVEETAQQPVMTPCKIEPSINHVLSTRKPGKEEGDLLQRVQSHQQDSEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKEQREAELLTSAEKRAEMQKQIEEMEKKLKEIQTTQHERTGDQQEEMSTKETVELQIASVSQEIPGMTLSNSVCPINSCARETSLAENIWQEQPHSKDPSIPFSIFDEFLLSEKRNISPSADPPRVLVQRRPLAVLKTSENITSNEDVSPDVCDEFTGIEPLSEDAIITGFRNITICPNPEDTCDFASAARFVSTPFHEIISLKDLPSDSERLLQEEDLDVKTSEDQQTTCGTIYNPTLSIKKLSPIIEDSREATHSSGFSGSSASVISTCSLKCLQIPEKLELTNETAENPVQSPWCSQYRRQLLKSLPELSASVEIFVEDRPMPKLETEKEIELGNEDYCIKQEYLVCEDYKLFWVTPRNSTELTIIKVSSQTVPWDFYINLKLKERLNEECDHLCSYYQYQDGCIVWHQYINCFTLQDLFQHSEFITHEITVLIVYNLLTIVEKLHKAEIVHGDLSPRSLILRNRIHDPYDCNKNNQALNIVDFSYSVDLRAQLDIFALSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSLWKLSQNISELKDGELWNKFFVRILNASDESTVSVLRELAAEINWVFDTTFQSHLNKALWKVGKLIGPGALLFQQDRQSSLSQNPA

Target ORF information:

RefSeq Version	XM_006920498.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006920498.2

ORF Insert Sequence:

ATGGCAGCAA TGAAGAAGGA AGAGGGTGGA CCGAGTGAAG CCATGTCTCT GGAGGGAGAT 
GAATGGGAGC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AGGGGCGGAG CATGTCCACA 
CTTCAGGGAA TATTGGCACA GCAAGAATCT GCCCGTAACA CTACTCTACA GCAGCAGAAA 
CGGGCATTTG AATCTGAAAT TCGATTTTAC ACTGGAAATG ACCCTCTGGA TGTTTGGGAT 
AGGTATATTA ACTGGACAGA GCAGAACTAT CCTCAGGGGG GCAAGGAGAG TAATATGTCA 
ACATTATTAG AAAGAGCTGT AGAAGCACTA CAAGGAGAAA AAAGATATTA TAATGATCCT 
CGATTTCTCA GTCTCTGGCT TAAATTGGGA CATTTATGCA ATGAGCCTTT GGATATGTAC 
AGTTATTTAC ACAACCAAGG GATTGGCATT TCACTTGCTC AGTTCTATAT TTCATGGGCT 
GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCAGACA TGATATTTCA GGATGGAATT 
CAACAGAAGG CTGAACCACT GGAAAGACTG CAATCCCAGC ACCGACAATT CCAAGCTCGA 
GTGACTCGGC AAACTCTGAT GGCACTTGAG AAAGAAGAAG AGGAAGTTTT TGGAGCCTCT 
GTACCACAGC GAAGCACACT GGCTGAACTA AAAAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCAGCC GTATAGGAGG TGCTCTCAAG GCTCCAAACC AGAACCGAGG ACTCCAAAAT 
CCATTTCCTC AACCGATGCA AAGTAATTCT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA TAGCAGAGCT GTCTACGCCT ACAGTCCAGC CATGGATTGC ACCCCCTGTG 
TCCAGGGCCA AAGAAAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GCCTTTGGAA 
CACAGACCTC GTGGCAATAC AGCTTCTGTG ACAGCTGTAC CCTCTATGCT TCCCAGTTTT 
ACTCCGTATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCC 
AGTATAAACC ACGTTCTAAG CACAAGAAAG CCTGGAAAGG AAGAAGGGGA TCTCCTGCAA 
AGGGTTCAAA GCCATCAGCA AGATTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAAATTT ATGCAGGAGT TGGGGAGTTC TCCTTTGAAG AGATCCGGGC TGAAGTTTTC 
CGAAAGAAAT TAAAAGAGCA AAGGGAAGCT GAGCTACTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAAATTGA AGAGATGGAG AAGAAGCTGA AAGAAATTCA AACCACTCAG 
CATGAAAGAA CGGGTGATCA GCAAGAAGAG ATGTCTACAA AGGAGACAGT TGAACTGCAA 
ATTGCTTCAG TGTCTCAGGA AATCCCAGGA ATGACTCTAT CCAATTCTGT TTGTCCAATA 
AACTCTTGTG CCAGGGAAAC TTCACTTGCA GAAAACATTT GGCAAGAACA ACCTCATTCT 
AAAGATCCCA GTATACCTTT CTCCATTTTT GATGAGTTTC TTCTTTCAGA AAAAAGGAAT 
ATCAGTCCCT CTGCAGATCC CCCACGGGTT TTAGTCCAAC GAAGACCCCT TGCAGTTCTC 
AAAACTTCAG AAAACATCAC CTCAAATGAG GATGTGTCTC CAGATGTTTG TGATGAATTC 
ACAGGAATTG AGCCCTTGAG TGAAGATGCT ATTATCACAG GTTTCAGGAA TATAACTATT 
TGTCCTAACC CAGAAGACAC TTGTGACTTT GCTAGCGCAG CTCGTTTTGT ATCCACTCCT 
TTTCATGAGA TAATATCCTT GAAGGATCTC CCTTCTGACT CTGAGAGACT TTTGCAGGAA 
GAGGATCTAG ATGTGAAGAC CTCTGAGGAC CAACAGACTA CTTGTGGCAC TATCTACAAT 
CCAACTCTCA GCATCAAGAA GCTGAGCCCA ATTATTGAAG ATAGCCGCGA GGCCACACAC 
TCCTCTGGGT TCTCTGGATC TTCTGCCTCA GTTATAAGCA CCTGCTCCCT CAAATGCCTT 
CAAATTCCTG AGAAACTGGA GCTTACTAAT GAAACTGCAG AAAATCCTGT TCAGTCACCC 
TGGTGTTCAC AGTATCGTAG ACAACTACTA AAATCCCTAC CAGAGCTGAG TGCCTCTGTA 
GAGATTTTTG TAGAAGATAG ACCAATGCCT AAGTTGGAAA CAGAGAAGGA AATTGAATTA 
GGTAATGAGG ATTATTGCAT TAAACAAGAA TACCTAGTAT GTGAAGATTA CAAATTATTC 
TGGGTGACAC CAAGAAACTC TACAGAATTA ACCATAATAA AGGTATCTTC TCAAACTGTC 
CCATGGGACT TTTATATCAA CCTCAAGTTA AAGGAACGTT TAAATGAGGA GTGTGATCAT 
TTGTGCAGCT ATTATCAATA CCAAGATGGT TGTATTGTTT GGCACCAGTA TATAAACTGC 
TTCACCCTTC AGGATCTTTT TCAGCACAGT GAATTTATTA CCCATGAAAT AACAGTGTTG 
ATAGTTTATA ACCTTTTGAC AATAGTGGAG AAACTACACA AAGCGGAAAT AGTCCATGGT 
GACTTGAGTC CAAGGAGTCT GATTCTTAGA AACAGAATCC ACGATCCCTA TGATTGTAAT 
AAGAACAATC AAGCTTTGAA CATAGTGGAC TTTTCCTACA GTGTCGACCT CAGGGCTCAG 
CTAGACATTT TTGCCCTCAG TGGCTTTCGG ACTGTACAGA TCCTGGAAGG ACAAAAGATC 
CTGGCTAACT GTTCTTCTCC CTACCAGGTA GATCTGTTTG GTATAGCAGA TTTAGCACAT 
TTACTATTGT TCAAGGAACA TCTACAGGTC TTCTGGGATG GGTCCCTCTG GAAACTTAGC 
CAAAATATTT CTGAGCTAAA AGATGGTGAA TTGTGGAATA AATTCTTTGT GCGGATTCTG 
AATGCCAGTG ATGAGTCCAC AGTGTCTGTT CTGAGGGAAC TTGCAGCAGA AATCAATTGG 
GTGTTTGATA CCACATTTCA AAGTCACCTG AACAAAGCTT TATGGAAGGT AGGGAAGTTA 
ATCGGTCCTG GGGCTTTGCT CTTCCAGCAA GATAGGCAGT CAAGTCTCTC ACAGAATCCT 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1B ( XM_006920498.3 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006920560.1 Pteropus alecto BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

Price & Availability↑

CloneID	OPt24876
Clone ID Related Accession (Same CDS sequence)	XM_006920498.2 , XM_006920498.3
Accession Version	XM_006920498.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3186bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006442109.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_006920498.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCAA TGAAGAAGGA AGAGGGTGGA CCGAGTGAAG CCATGTCTCT GGAGGGAGAT 
GAATGGGAGC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AGGGGCGGAG CATGTCCACA 
CTTCAGGGAA TATTGGCACA GCAAGAATCT GCCCGTAACA CTACTCTACA GCAGCAGAAA 
CGGGCATTTG AATCTGAAAT TCGATTTTAC ACTGGAAATG ACCCTCTGGA TGTTTGGGAT 
AGGTATATTA ACTGGACAGA GCAGAACTAT CCTCAGGGGG GCAAGGAGAG TAATATGTCA 
ACATTATTAG AAAGAGCTGT AGAAGCACTA CAAGGAGAAA AAAGATATTA TAATGATCCT 
CGATTTCTCA GTCTCTGGCT TAAATTGGGA CATTTATGCA ATGAGCCTTT GGATATGTAC 
AGTTATTTAC ACAACCAAGG GATTGGCATT TCACTTGCTC AGTTCTATAT TTCATGGGCT 
GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCAGACA TGATATTTCA GGATGGAATT 
CAACAGAAGG CTGAACCACT GGAAAGACTG CAATCCCAGC ACCGACAATT CCAAGCTCGA 
GTGACTCGGC AAACTCTGAT GGCACTTGAG AAAGAAGAAG AGGAAGTTTT TGGAGCCTCT 
GTACCACAGC GAAGCACACT GGCTGAACTA AAAAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCAGCC GTATAGGAGG TGCTCTCAAG GCTCCAAACC AGAACCGAGG ACTCCAAAAT 
CCATTTCCTC AACCGATGCA AAGTAATTCT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA TAGCAGAGCT GTCTACGCCT ACAGTCCAGC CATGGATTGC ACCCCCTGTG 
TCCAGGGCCA AAGAAAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GCCTTTGGAA 
CACAGACCTC GTGGCAATAC AGCTTCTGTG ACAGCTGTAC CCTCTATGCT TCCCAGTTTT 
ACTCCGTATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCC 
AGTATAAACC ACGTTCTAAG CACAAGAAAG CCTGGAAAGG AAGAAGGGGA TCTCCTGCAA 
AGGGTTCAAA GCCATCAGCA AGATTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAAATTT ATGCAGGAGT TGGGGAGTTC TCCTTTGAAG AGATCCGGGC TGAAGTTTTC 
CGAAAGAAAT TAAAAGAGCA AAGGGAAGCT GAGCTACTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAAATTGA AGAGATGGAG AAGAAGCTGA AAGAAATTCA AACCACTCAG 
CATGAAAGAA CGGGTGATCA GCAAGAAGAG ATGTCTACAA AGGAGACAGT TGAACTGCAA 
ATTGCTTCAG TGTCTCAGGA AATCCCAGGA ATGACTCTAT CCAATTCTGT TTGTCCAATA 
AACTCTTGTG CCAGGGAAAC TTCACTTGCA GAAAACATTT GGCAAGAACA ACCTCATTCT 
AAAGATCCCA GTATACCTTT CTCCATTTTT GATGAGTTTC TTCTTTCAGA AAAAAGGAAT 
ATCAGTCCCT CTGCAGATCC CCCACGGGTT TTAGTCCAAC GAAGACCCCT TGCAGTTCTC 
AAAACTTCAG AAAACATCAC CTCAAATGAG GATGTGTCTC CAGATGTTTG TGATGAATTC 
ACAGGAATTG AGCCCTTGAG TGAAGATGCT ATTATCACAG GTTTCAGGAA TATAACTATT 
TGTCCTAACC CAGAAGACAC TTGTGACTTT GCTAGCGCAG CTCGTTTTGT ATCCACTCCT 
TTTCATGAGA TAATATCCTT GAAGGATCTC CCTTCTGACT CTGAGAGACT TTTGCAGGAA 
GAGGATCTAG ATGTGAAGAC CTCTGAGGAC CAACAGACTA CTTGTGGCAC TATCTACAAT 
CCAACTCTCA GCATCAAGAA GCTGAGCCCA ATTATTGAAG ATAGCCGCGA GGCCACACAC 
TCCTCTGGGT TCTCTGGATC TTCTGCCTCA GTTATAAGCA CCTGCTCCCT CAAATGCCTT 
CAAATTCCTG AGAAACTGGA GCTTACTAAT GAAACTGCAG AAAATCCTGT TCAGTCACCC 
TGGTGTTCAC AGTATCGTAG ACAACTACTA AAATCCCTAC CAGAGCTGAG TGCCTCTGTA 
GAGATTTTTG TAGAAGATAG ACCAATGCCT AAGTTGGAAA CAGAGAAGGA AATTGAATTA 
GGTAATGAGG ATTATTGCAT TAAACAAGAA TACCTAGTAT GTGAAGATTA CAAATTATTC 
TGGGTGACAC CAAGAAACTC TACAGAATTA ACCATAATAA AGGTATCTTC TCAAACTGTC 
CCATGGGACT TTTATATCAA CCTCAAGTTA AAGGAACGTT TAAATGAGGA GTGTGATCAT 
TTGTGCAGCT ATTATCAATA CCAAGATGGT TGTATTGTTT GGCACCAGTA TATAAACTGC 
TTCACCCTTC AGGATCTTTT TCAGCACAGT GAATTTATTA CCCATGAAAT AACAGTGTTG 
ATAGTTTATA ACCTTTTGAC AATAGTGGAG AAACTACACA AAGCGGAAAT AGTCCATGGT 
GACTTGAGTC CAAGGAGTCT GATTCTTAGA AACAGAATCC ACGATCCCTA TGATTGTAAT 
AAGAACAATC AAGCTTTGAA CATAGTGGAC TTTTCCTACA GTGTCGACCT CAGGGCTCAG 
CTAGACATTT TTGCCCTCAG TGGCTTTCGG ACTGTACAGA TCCTGGAAGG ACAAAAGATC 
CTGGCTAACT GTTCTTCTCC CTACCAGGTA GATCTGTTTG GTATAGCAGA TTTAGCACAT 
TTACTATTGT TCAAGGAACA TCTACAGGTC TTCTGGGATG GGTCCCTCTG GAAACTTAGC 
CAAAATATTT CTGAGCTAAA AGATGGTGAA TTGTGGAATA AATTCTTTGT GCGGATTCTG 
AATGCCAGTG ATGAGTCCAC AGTGTCTGTT CTGAGGGAAC TTGCAGCAGA AATCAATTGG 
GTGTTTGATA CCACATTTCA AAGTCACCTG AACAAAGCTT TATGGAAGGT AGGGAAGTTA 
ATCGGTCCTG GGGCTTTGCT CTTCCAGCAA GATAGGCAGT CAAGTCTCTC ACAGAATCCT 
GCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006920560.1
CDS	220..3405
Translation	MAAMKKEEGGPSEAMSLEGDEWELSKENVQPLRQGRSMSTLQGILAQQESARNTTLQQQKRAFESEIRFYTGNDPLDVWDRYINWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYNDPRFLSLWLKLGHLCNEPLDMYSYLHNQGIGISLAQFYISWAEEYEARENFKKADMIFQDGIQQKAEPLERLQSQHRQFQARVTRQTLMALEKEEEEVFGASVPQRSTLAELKSKGKKTARAPISRIGGALKAPNQNRGLQNPFPQPMQSNSRITVFDENADEASIAELSTPTVQPWIAPPVSRAKENELQAGPWNTGRPLEHRPRGNTASVTAVPSMLPSFTPYVEETAQQPVMTPCKIEPSINHVLSTRKPGKEEGDLLQRVQSHQQDSEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKEQREAELLTSAEKRAEMQKQIEEMEKKLKEIQTTQHERTGDQQEEMSTKETVELQIASVSQEIPGMTLSNSVCPINSCARETSLAENIWQEQPHSKDPSIPFSIFDEFLLSEKRNISPSADPPRVLVQRRPLAVLKTSENITSNEDVSPDVCDEFTGIEPLSEDAIITGFRNITICPNPEDTCDFASAARFVSTPFHEIISLKDLPSDSERLLQEEDLDVKTSEDQQTTCGTIYNPTLSIKKLSPIIEDSREATHSSGFSGSSASVISTCSLKCLQIPEKLELTNETAENPVQSPWCSQYRRQLLKSLPELSASVEIFVEDRPMPKLETEKEIELGNEDYCIKQEYLVCEDYKLFWVTPRNSTELTIIKVSSQTVPWDFYINLKLKERLNEECDHLCSYYQYQDGCIVWHQYINCFTLQDLFQHSEFITHEITVLIVYNLLTIVEKLHKAEIVHGDLSPRSLILRNRIHDPYDCNKNNQALNIVDFSYSVDLRAQLDIFALSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSLWKLSQNISELKDGELWNKFFVRILNASDESTVSVLRELAAEINWVFDTTFQSHLNKALWKVGKLIGPGALLFQQDRQSSLSQNPA

Target ORF information:

RefSeq Version	XM_006920498.3
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006920498.3

ORF Insert Sequence:

ATGGCAGCAA TGAAGAAGGA AGAGGGTGGA CCGAGTGAAG CCATGTCTCT GGAGGGAGAT 
GAATGGGAGC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AGGGGCGGAG CATGTCCACA 
CTTCAGGGAA TATTGGCACA GCAAGAATCT GCCCGTAACA CTACTCTACA GCAGCAGAAA 
CGGGCATTTG AATCTGAAAT TCGATTTTAC ACTGGAAATG ACCCTCTGGA TGTTTGGGAT 
AGGTATATTA ACTGGACAGA GCAGAACTAT CCTCAGGGGG GCAAGGAGAG TAATATGTCA 
ACATTATTAG AAAGAGCTGT AGAAGCACTA CAAGGAGAAA AAAGATATTA TAATGATCCT 
CGATTTCTCA GTCTCTGGCT TAAATTGGGA CATTTATGCA ATGAGCCTTT GGATATGTAC 
AGTTATTTAC ACAACCAAGG GATTGGCATT TCACTTGCTC AGTTCTATAT TTCATGGGCT 
GAAGAATATG AAGCTAGAGA AAACTTTAAG AAAGCAGACA TGATATTTCA GGATGGAATT 
CAACAGAAGG CTGAACCACT GGAAAGACTG CAATCCCAGC ACCGACAATT CCAAGCTCGA 
GTGACTCGGC AAACTCTGAT GGCACTTGAG AAAGAAGAAG AGGAAGTTTT TGGAGCCTCT 
GTACCACAGC GAAGCACACT GGCTGAACTA AAAAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCAGCC GTATAGGAGG TGCTCTCAAG GCTCCAAACC AGAACCGAGG ACTCCAAAAT 
CCATTTCCTC AACCGATGCA AAGTAATTCT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA TAGCAGAGCT GTCTACGCCT ACAGTCCAGC CATGGATTGC ACCCCCTGTG 
TCCAGGGCCA AAGAAAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GCCTTTGGAA 
CACAGACCTC GTGGCAATAC AGCTTCTGTG ACAGCTGTAC CCTCTATGCT TCCCAGTTTT 
ACTCCGTATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCC 
AGTATAAACC ACGTTCTAAG CACAAGAAAG CCTGGAAAGG AAGAAGGGGA TCTCCTGCAA 
AGGGTTCAAA GCCATCAGCA AGATTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAAATTT ATGCAGGAGT TGGGGAGTTC TCCTTTGAAG AGATCCGGGC TGAAGTTTTC 
CGAAAGAAAT TAAAAGAGCA AAGGGAAGCT GAGCTACTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAAATTGA AGAGATGGAG AAGAAGCTGA AAGAAATTCA AACCACTCAG 
CATGAAAGAA CGGGTGATCA GCAAGAAGAG ATGTCTACAA AGGAGACAGT TGAACTGCAA 
ATTGCTTCAG TGTCTCAGGA AATCCCAGGA ATGACTCTAT CCAATTCTGT TTGTCCAATA 
AACTCTTGTG CCAGGGAAAC TTCACTTGCA GAAAACATTT GGCAAGAACA ACCTCATTCT 
AAAGATCCCA GTATACCTTT CTCCATTTTT GATGAGTTTC TTCTTTCAGA AAAAAGGAAT 
ATCAGTCCCT CTGCAGATCC CCCACGGGTT TTAGTCCAAC GAAGACCCCT TGCAGTTCTC 
AAAACTTCAG AAAACATCAC CTCAAATGAG GATGTGTCTC CAGATGTTTG TGATGAATTC 
ACAGGAATTG AGCCCTTGAG TGAAGATGCT ATTATCACAG GTTTCAGGAA TATAACTATT 
TGTCCTAACC CAGAAGACAC TTGTGACTTT GCTAGCGCAG CTCGTTTTGT ATCCACTCCT 
TTTCATGAGA TAATATCCTT GAAGGATCTC CCTTCTGACT CTGAGAGACT TTTGCAGGAA 
GAGGATCTAG ATGTGAAGAC CTCTGAGGAC CAACAGACTA CTTGTGGCAC TATCTACAAT 
CCAACTCTCA GCATCAAGAA GCTGAGCCCA ATTATTGAAG ATAGCCGCGA GGCCACACAC 
TCCTCTGGGT TCTCTGGATC TTCTGCCTCA GTTATAAGCA CCTGCTCCCT CAAATGCCTT 
CAAATTCCTG AGAAACTGGA GCTTACTAAT GAAACTGCAG AAAATCCTGT TCAGTCACCC 
TGGTGTTCAC AGTATCGTAG ACAACTACTA AAATCCCTAC CAGAGCTGAG TGCCTCTGTA 
GAGATTTTTG TAGAAGATAG ACCAATGCCT AAGTTGGAAA CAGAGAAGGA AATTGAATTA 
GGTAATGAGG ATTATTGCAT TAAACAAGAA TACCTAGTAT GTGAAGATTA CAAATTATTC 
TGGGTGACAC CAAGAAACTC TACAGAATTA ACCATAATAA AGGTATCTTC TCAAACTGTC 
CCATGGGACT TTTATATCAA CCTCAAGTTA AAGGAACGTT TAAATGAGGA GTGTGATCAT 
TTGTGCAGCT ATTATCAATA CCAAGATGGT TGTATTGTTT GGCACCAGTA TATAAACTGC 
TTCACCCTTC AGGATCTTTT TCAGCACAGT GAATTTATTA CCCATGAAAT AACAGTGTTG 
ATAGTTTATA ACCTTTTGAC AATAGTGGAG AAACTACACA AAGCGGAAAT AGTCCATGGT 
GACTTGAGTC CAAGGAGTCT GATTCTTAGA AACAGAATCC ACGATCCCTA TGATTGTAAT 
AAGAACAATC AAGCTTTGAA CATAGTGGAC TTTTCCTACA GTGTCGACCT CAGGGCTCAG 
CTAGACATTT TTGCCCTCAG TGGCTTTCGG ACTGTACAGA TCCTGGAAGG ACAAAAGATC 
CTGGCTAACT GTTCTTCTCC CTACCAGGTA GATCTGTTTG GTATAGCAGA TTTAGCACAT 
TTACTATTGT TCAAGGAACA TCTACAGGTC TTCTGGGATG GGTCCCTCTG GAAACTTAGC 
CAAAATATTT CTGAGCTAAA AGATGGTGAA TTGTGGAATA AATTCTTTGT GCGGATTCTG 
AATGCCAGTG ATGAGTCCAC AGTGTCTGTT CTGAGGGAAC TTGCAGCAGA AATCAATTGG 
GTGTTTGATA CCACATTTCA AAGTCACCTG AACAAAGCTT TATGGAAGGT AGGGAAGTTA 
ATCGGTCCTG GGGCTTTGCT CTTCCAGCAA GATAGGCAGT CAAGTCTCTC ACAGAATCCT 
GCCTAA