HK2 cDNA ORF clone, Pteropus alecto(black flying fox)

Gene
Clones
gRNAs

Summary

Gene Symbol	HK2
Entrez Gene ID	102892478
Full Name	hexokinase 2
Gene Type	protein-coding
Organism	Pteropus alecto(black flying fox)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025049464.1	XP_024905232.1	LOW QUALITY PROTEIN: hexokinase-2
XM_006905226.2	XP_006905288.1	hexokinase-2

Pathways from BioSystems
Gene Ontology

The following HK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OPt06635	XM_006905226.2 Latest version!	Pteropus alecto hexokinase 2 (HK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OPt80496	XM_025049464.1 Latest version!	Pteropus alecto hexokinase 2 (HK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HK2 ( XM_006905226.2 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_006905288.1 Pteropus alecto hexokinase 2 (HK2), mRNA.

Price & Availability↑

CloneID	OPt06635
Clone ID Related Accession (Same CDS sequence)	XM_006905226.2
Accession Version	XM_006905226.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2754bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-02-09
Organism	Pteropus alecto(black flying fox)
Product	hexokinase-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006430723.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Feb 10, 2016 this sequence version replaced XM_006905226.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pteropus alecto Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCGCCT CGCATCTGCT CGCCTACTTC TTCACGGAGC TCAACCATGA CCAAGTGCAG 
AAGGTTGACC AGTATCTCTA CCATATGCGT CTTTCTGATG AGACCCTTCT GGAGATCTCT 
AAGCGGTTCC GCAAGGAGAT GGAAAAAGGG CTTGGAGCCA CCACCCACCC CACTGCTTCC 
GTGAAAATGC TGCCCACCTT CGTGAGGTCT ACTCCAGACG GGACAGAACA CGGAGAGTTC 
CTGGCTCTAG ACCTTGGAGG GACTAATTTC CGCGTCCTTC GGGTGAGAGT AACGGACAAT 
GGGCTCCAGA AGGTCGAGAT GGAGAACCAG ATCTACGCCA TCCCCGAGGA CCTCATGCGA 
GGCAGCGGCA CCCAGCTGTT CGACCACATT GCTGAATGCC TGGCTAACTT CATGGATACG 
TTACAAATCA AAGATAAGAA GCTCCCCTTG GGCTTTACCT TTTCGTTCCC CTGCATCCAG 
ACAAAACTAG ATGAGAGCTT CCTGGTCTCG TGGACTAAGG GGTTCAAGTC CAGCGGTGTG 
GAAGGCAAAG ATGTGGTTAC TCTAATACGG AAGGCCATCC AGAGGAGAGG GGACTTTGAT 
ATTGATATCG TGGCTGTGGT GAATGACACG GTTGGGACCA TGATGACCTG TGGTTATGAT 
GACCAGAACT GCGAGATTGG TCTCATTGTG GGCACGGGCA GCAACGCCTG CTACATGGAG 
GAGATGCGCC ACATTGACAC AGTGGAAGGT GACGAGGGCC GGATGTGCAT CAACATGGAG 
TGGGGCGCCT TCGGGGACGA CGGCGTGCTT GATGACATCC GTACCGAGTT TGACCACGAG 
ATCGACATGG GCTCCTTGAA CCCCGGGAAG CAACTATTTG AGAAGATGAT CAGTGGGATG 
TACATGGGGG AACTGGTGAG GCTAATCCTG GTGAAGATGG CCAAGGAGGA GCTGCTTTTC 
AGAGGGAAGC TCAGCCCCGA ACTCCTTGCC ACAGGCCGCT TTGAGACAAA AGATGTTTCC 
GACATAGAAG GGGAGAAGGA TGGCATCCGG AAGGCTCGCG AGATCCTGGT GCGGCTGGGC 
TTGGACCCGA CACAGGAGGA CTGCGTGGCC ACCCACCGGA TCTGCCAGAT TGTGTCCACG 
CGCTCAGCCA ACCTGTGCGC GGCCACCCTG GCAGCCGTGC TGCGGCGCAT CAAGGAGAAC 
AAGGGCGAGG AGCGGCTGCG GTCCACCATC GGGGTGGACG GCTCCGTCTA CAAGAAGCAC 
CCCCATTTCG CCAAGCGTCT TCACAAGACT GTGCGGCGCC TGGTGCCCGA CTGCGATGTC 
CGCTTCCTCC GCTCTGAGGA TGGCAGTGGC AAGGGGGCAG CCATGGTGAC GGCAGTGGCC 
CACCGGCTGG CCGACCAACA CCGAGCCCGC CAGAAGACCC TGGAGCCTCT GAAGCTGAGC 
CGTGAGCAGT TGCTGGAGGT CAAGAGGAGG ATGAAGGTAG AAATGGAGCG AGGTCTGAGC 
AAGGAGACTC ACGCCATTGC CCCCGTCAAG ATGCTGCCCA CCTACGTGTG TGCCACCCCT 
GATGGCACAG AGAAAGGTGA CTTCCTGGCC TTGGACCTTG GGGGCACCAA TTTCCGGGTC 
CTGCTGGTGC GTGTGCGGAA TGGGAAGCGG CGCGGAGTGG AGATGCATAA CAAGATCTAC 
ACCATCCCGC AGGAGGTCAT GCACGGCACA GGGGACGAGC TCTTCGACCA CATTGTCCAG 
TGCATCGCCG ACTTCCTTGA GTACATGGGC ATGAAGGGCG TGTCTCTGCC GCTGGGCTTC 
ACCTTCTCCT TCCCGTGCAA ACAGAACAGC CTGGACGAGA GCATCCTCCT CAAGTGGACT 
AAAGGCTTCA AGGCATCTGG CTGTGAGGGC GAGGATGTGG TCACCCTGCT GAAGGAAGCC 
ATCCACCGGC GAGAGGAGTT CGACCTGGAT GTGGTAGCCG TGGTGAATGA CACAGTCGGG 
ACTATGATGA CCTGTGGCTA TGAAGACCCT CACTGTGAAG TCGGCCTCAT CATCGGCACG 
GGCTGCAACG CCTGCTACAT GGAGGAGATG AGGAATGTGG AGCTGGTGGA AGGGGACGAG 
GGGCGGATGT GTGTCAACAT GGAGTGGGGG GCCTTTGGGG ACAATGGATG CCTAGAGGAC 
CTCCGCACGG AATTTGATGT GGCTGTGGAT GAGCTGTCCC TCAACTTTGG CAAACAGAGG 
TTTGAGAAAA TGATCAGTGG CATGTACTTG GGCGAGATTG TCCGTAATAT CCTTATCGAT 
TTCACCAAGC GTGGGCTGCT CTTCCGTGGC CGCATCTCAG AGCGGCTCAA GACCAGAGGA 
ATCTTTGAAA CCAAGTTCCT GTCTCAGATT GAGAGTGACT GCCTGGCCCT GCTACAAGTT 
CGTGCCATCC TGCGCCACTT AGGGCTCGAG AGCACCTGTG ACGACAGCAT CATTGTCAAG 
GAGGTGTGCA CGGTGGTGGC GCGGCGGGCA GCCCAGCTCT GTGGTGCAGG CCTGGCTGCT 
GTGGTGGACA AAATACGAGA GAACCGTGGA CTGGACACTC TCAAAGTGAC GGTGGGCGTG 
GATGGGACCC TCTATAAGCT ACATCCTCAC TTTGCCAAAG TCATGCATGA GACAGTGAAG 
GACCTGGCTC CGAAATGTGA CGTGTCTTTC CTGGCTTCAG AGGACGGCAG TGGGAAGGGG 
GCGGCTCTCA TCACTGCTGT GGCCTGTCGC ATCCGTGAGG CTGGACAGCG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006905288.1
CDS	465..3218
Translation	MIASHLLAYFFTELNHDQVQKVDQYLYHMRLSDETLLEISKRFRKEMEKGLGATTHPTASVKMLPTFVRSTPDGTEHGEFLALDLGGTNFRVLRVRVTDNGLQKVEMENQIYAIPEDLMRGSGTQLFDHIAECLANFMDTLQIKDKKLPLGFTFSFPCIQTKLDESFLVSWTKGFKSSGVEGKDVVTLIRKAIQRRGDFDIDIVAVVNDTVGTMMTCGYDDQNCEIGLIVGTGSNACYMEEMRHIDTVEGDEGRMCINMEWGAFGDDGVLDDIRTEFDHEIDMGSLNPGKQLFEKMISGMYMGELVRLILVKMAKEELLFRGKLSPELLATGRFETKDVSDIEGEKDGIRKAREILVRLGLDPTQEDCVATHRICQIVSTRSANLCAATLAAVLRRIKENKGEERLRSTIGVDGSVYKKHPHFAKRLHKTVRRLVPDCDVRFLRSEDGSGKGAAMVTAVAHRLADQHRARQKTLEPLKLSREQLLEVKRRMKVEMERGLSKETHAIAPVKMLPTYVCATPDGTEKGDFLALDLGGTNFRVLLVRVRNGKRRGVEMHNKIYTIPQEVMHGTGDELFDHIVQCIADFLEYMGMKGVSLPLGFTFSFPCKQNSLDESILLKWTKGFKASGCEGEDVVTLLKEAIHRREEFDLDVVAVVNDTVGTMMTCGYEDPHCEVGLIIGTGCNACYMEEMRNVELVEGDEGRMCVNMEWGAFGDNGCLEDLRTEFDVAVDELSLNFGKQRFEKMISGMYLGEIVRNILIDFTKRGLLFRGRISERLKTRGIFETKFLSQIESDCLALLQVRAILRHLGLESTCDDSIIVKEVCTVVARRAAQLCGAGLAAVVDKIRENRGLDTLKVTVGVDGTLYKLHPHFAKVMHETVKDLAPKCDVSFLASEDGSGKGAALITAVACRIREAGQR

Target ORF information:

RefSeq Version	XM_006905226.2
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto hexokinase 2 (HK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006905226.2

ORF Insert Sequence:

ATGATCGCCT CGCATCTGCT CGCCTACTTC TTCACGGAGC TCAACCATGA CCAAGTGCAG 
AAGGTTGACC AGTATCTCTA CCATATGCGT CTTTCTGATG AGACCCTTCT GGAGATCTCT 
AAGCGGTTCC GCAAGGAGAT GGAAAAAGGG CTTGGAGCCA CCACCCACCC CACTGCTTCC 
GTGAAAATGC TGCCCACCTT CGTGAGGTCT ACTCCAGACG GGACAGAACA CGGAGAGTTC 
CTGGCTCTAG ACCTTGGAGG GACTAATTTC CGCGTCCTTC GGGTGAGAGT AACGGACAAT 
GGGCTCCAGA AGGTCGAGAT GGAGAACCAG ATCTACGCCA TCCCCGAGGA CCTCATGCGA 
GGCAGCGGCA CCCAGCTGTT CGACCACATT GCTGAATGCC TGGCTAACTT CATGGATACG 
TTACAAATCA AAGATAAGAA GCTCCCCTTG GGCTTTACCT TTTCGTTCCC CTGCATCCAG 
ACAAAACTAG ATGAGAGCTT CCTGGTCTCG TGGACTAAGG GGTTCAAGTC CAGCGGTGTG 
GAAGGCAAAG ATGTGGTTAC TCTAATACGG AAGGCCATCC AGAGGAGAGG GGACTTTGAT 
ATTGATATCG TGGCTGTGGT GAATGACACG GTTGGGACCA TGATGACCTG TGGTTATGAT 
GACCAGAACT GCGAGATTGG TCTCATTGTG GGCACGGGCA GCAACGCCTG CTACATGGAG 
GAGATGCGCC ACATTGACAC AGTGGAAGGT GACGAGGGCC GGATGTGCAT CAACATGGAG 
TGGGGCGCCT TCGGGGACGA CGGCGTGCTT GATGACATCC GTACCGAGTT TGACCACGAG 
ATCGACATGG GCTCCTTGAA CCCCGGGAAG CAACTATTTG AGAAGATGAT CAGTGGGATG 
TACATGGGGG AACTGGTGAG GCTAATCCTG GTGAAGATGG CCAAGGAGGA GCTGCTTTTC 
AGAGGGAAGC TCAGCCCCGA ACTCCTTGCC ACAGGCCGCT TTGAGACAAA AGATGTTTCC 
GACATAGAAG GGGAGAAGGA TGGCATCCGG AAGGCTCGCG AGATCCTGGT GCGGCTGGGC 
TTGGACCCGA CACAGGAGGA CTGCGTGGCC ACCCACCGGA TCTGCCAGAT TGTGTCCACG 
CGCTCAGCCA ACCTGTGCGC GGCCACCCTG GCAGCCGTGC TGCGGCGCAT CAAGGAGAAC 
AAGGGCGAGG AGCGGCTGCG GTCCACCATC GGGGTGGACG GCTCCGTCTA CAAGAAGCAC 
CCCCATTTCG CCAAGCGTCT TCACAAGACT GTGCGGCGCC TGGTGCCCGA CTGCGATGTC 
CGCTTCCTCC GCTCTGAGGA TGGCAGTGGC AAGGGGGCAG CCATGGTGAC GGCAGTGGCC 
CACCGGCTGG CCGACCAACA CCGAGCCCGC CAGAAGACCC TGGAGCCTCT GAAGCTGAGC 
CGTGAGCAGT TGCTGGAGGT CAAGAGGAGG ATGAAGGTAG AAATGGAGCG AGGTCTGAGC 
AAGGAGACTC ACGCCATTGC CCCCGTCAAG ATGCTGCCCA CCTACGTGTG TGCCACCCCT 
GATGGCACAG AGAAAGGTGA CTTCCTGGCC TTGGACCTTG GGGGCACCAA TTTCCGGGTC 
CTGCTGGTGC GTGTGCGGAA TGGGAAGCGG CGCGGAGTGG AGATGCATAA CAAGATCTAC 
ACCATCCCGC AGGAGGTCAT GCACGGCACA GGGGACGAGC TCTTCGACCA CATTGTCCAG 
TGCATCGCCG ACTTCCTTGA GTACATGGGC ATGAAGGGCG TGTCTCTGCC GCTGGGCTTC 
ACCTTCTCCT TCCCGTGCAA ACAGAACAGC CTGGACGAGA GCATCCTCCT CAAGTGGACT 
AAAGGCTTCA AGGCATCTGG CTGTGAGGGC GAGGATGTGG TCACCCTGCT GAAGGAAGCC 
ATCCACCGGC GAGAGGAGTT CGACCTGGAT GTGGTAGCCG TGGTGAATGA CACAGTCGGG 
ACTATGATGA CCTGTGGCTA TGAAGACCCT CACTGTGAAG TCGGCCTCAT CATCGGCACG 
GGCTGCAACG CCTGCTACAT GGAGGAGATG AGGAATGTGG AGCTGGTGGA AGGGGACGAG 
GGGCGGATGT GTGTCAACAT GGAGTGGGGG GCCTTTGGGG ACAATGGATG CCTAGAGGAC 
CTCCGCACGG AATTTGATGT GGCTGTGGAT GAGCTGTCCC TCAACTTTGG CAAACAGAGG 
TTTGAGAAAA TGATCAGTGG CATGTACTTG GGCGAGATTG TCCGTAATAT CCTTATCGAT 
TTCACCAAGC GTGGGCTGCT CTTCCGTGGC CGCATCTCAG AGCGGCTCAA GACCAGAGGA 
ATCTTTGAAA CCAAGTTCCT GTCTCAGATT GAGAGTGACT GCCTGGCCCT GCTACAAGTT 
CGTGCCATCC TGCGCCACTT AGGGCTCGAG AGCACCTGTG ACGACAGCAT CATTGTCAAG 
GAGGTGTGCA CGGTGGTGGC GCGGCGGGCA GCCCAGCTCT GTGGTGCAGG CCTGGCTGCT 
GTGGTGGACA AAATACGAGA GAACCGTGGA CTGGACACTC TCAAAGTGAC GGTGGGCGTG 
GATGGGACCC TCTATAAGCT ACATCCTCAC TTTGCCAAAG TCATGCATGA GACAGTGAAG 
GACCTGGCTC CGAAATGTGA CGTGTCTTTC CTGGCTTCAG AGGACGGCAG TGGGAAGGGG 
GCGGCTCTCA TCACTGCTGT GGCCTGTCGC ATCCGTGAGG CTGGACAGCG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HK2 ( XM_025049464.1 ) cDNA ORF clone, Pteropus alecto(black flying fox) -> XP_024905232.1 Pteropus alecto hexokinase 2 (HK2), mRNA.

Price & Availability↑

CloneID	OPt80496
Clone ID Related Accession (Same CDS sequence)	XM_025049464.1
Accession Version	XM_025049464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Pteropus alecto(black flying fox)
Product	LOW QUALITY PROTEIN: hexokinase-2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006430723.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pteropus alecto Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGATCGCCT CGCATCTGCT CGCCTACTTC TTCACGGAGC TCAACCATGA CCAAGTGCAG 
AAGGTTGACC AGTATCTCTA CCATATGCGT CTTTCTGATG AGACCCTTCT GGAGATCTCT 
AAGCGGTTCC GCAAGGAGAT GGAAAAAGGG CTTGGAGCCA CCACCCACCC CACTGCTTCC 
GTGAAAATGC TGCCCACCTT CGTGAGGTCT ACTCCAGACG GGACAGAACA CGGAGAGTTC 
CTGGCTCTAG ACCTTGGAGG GACTAATTTC CGCGTCCTTC GGGTGAGAGT AACGGACAAT 
GGGCTCCAGA AGGTCGAGAT GGAGAACCAG ATCTACGCCA TCCCCGAGGA CCTCATGCGA 
GGCAGCGGCA CCCAGCTGTT CGACCACATT GCTGAATGCC TGGCTAACTT CATGGATACG 
TTACAAATCA AAGATAAGAA GCTCCCCTTG GGCTTTACCT TTTCGTTCCC CTGCATCCAG 
ACAAAACTAG ATGAGAGCTT CCTGGTCTCG TGGACTAAGG GGTTCAAGTC CAGCGGTGTG 
GAAGGCAAAG ATGTGGTTAC TCTAATACGG AAGGCCATCC AGAGGAGAGG GGACTTTGAT 
ATTGATATCG TGGCTGTGGT GAATGACACG GTTGGGACCA TGATGACCTG TGGTTATGAT 
GACCAGAACT GCGAGATTGG TCTCATTGTG GGCACGGGCA GCAACGCCTG CTACATGGAG 
GAGATGCGCC ACATTGACAC AGTGGAAGGT GACGAGGGCC GGATGTGCAT CAACATGGAG 
TGGGGCGCCT TCGGGGACGA CGGCGTGCTT GATGACATCC GTACCGAGTT TGACCACGAG 
ATCGACATGG GCTCCTTGAA CCCCGGGAAG CAACTATTTG AGAAGATGAT CAGTGGGATG 
TACATGGGGG AACTGGTGAG GCTAATCCTG GTGAAGATGG CCAAGGAGGA GCTGCTTTTC 
AGAGGGAAGC TCAGCCCCGA ACTCCTTGCC ACAGGCCGCT TTGAGACAAA AGATGTTTCC 
GACATAGAAG GGGAGAAGGA TGGCATCCGG AAGGCTCGCG AGATCCTGGT GCGGCTGGGC 
TTGGACCCGA CACAGGAGGA CTGCGTGGCC ACCCACCGGA TCTGCCAGAT TGTGTCCACG 
CGCTCAGCCA ACCTGTGCGC GGCCACCCTG GCAGCCGTGC TGCGGCGCAT CAAGGAGAAC 
AAGGGCGAGG AGCGGCTGCG GTCCACCATC GGGGTGGACG GCTCCGTCTA CAAGAAGCAC 
CCCCATTTCG CCAAGCGTCT TCACAAGACT GTGCGGCGCC TGGTGCCCGA CTGCGATGTC 
CGCTTCCTCC GCTCTGAGGA TGGCAGTGGC AAGGGGGCAG CCATGGTGAC GGCAGTGGCC 
CACCGGCTGG CCGACCAACA CCGAGCCCGC CAGAAGACCC TGGAGCCTCT GAAGCTGAGC 
CGTGAGCAGT TGCTGGAGGT CAAGAGGAGG ATGAAGGTAG AAATGGAGCG AGGTCTGAGC 
AAGGAGACTC ACGCCATTGC CCCCGTCAAG ATGCTGCCCA CCTACGTGTG TGCCACCCCT 
GATGGCACAG AGAAAGGTGA CTTCCTGGCC TTGGACCTTG GGGGCACCAA TTTCCGGGTC 
CTGCTGGTGC GTGTGCGGAA TGGGAAGCGG CGCGGAGTGG AGATGCATAA CAAGATCTAC 
ACCATCCCGC AGGAGGTCAT GCACGGCACA GGGGACGAGC TCTTCGACCA CATTGTCCAG 
TGCATCGCCG ACTTCCTTGA GTACATGGGC ATGAAGGGCG TGTCTCTGCC GCTGGGCTTC 
ACCTTCTCCT TCCCGTGCAA ACAGAACAGC CTGGACGAGA GCATCCTCCT CAAGTGGACT 
AAAGGCTTCA AGGCATCTGG CTGTGAGGGC GAGGATGTGG TCACCCTGCT GAAGGAAGCC 
ATCCACCGGC GAGAGGAGTT CGACCTGGAT GTGGTAGCCG TGGTGAATGA CACAGTCGGG 
ACTATGATGA CCTGTGGCTA TGAAGACCCT CACTGTGAAG TCGGCCTCAT CATCGGCACG 
GGCTGCAACG CCTGCTACAT GGAGGAGATG AGGAATGTGG AGCTGGTGGA AGGGGACGAG 
GGGCGGATGT GTGTCAACAT GGAGTGGGGG GCCTTTGGGG ACAATGGATG CCTAGAGGAC 
CTCCGCACGG AATTTGATGT GGCTGTGGAT GAGCTGTCCC TCAACTTTGG CAAACAGAGG 
TGGTCCACTG TATCTTAGCA AATGATCAGT GGCATGTACT TGGGCGAGAT TGTCCGTAAT 
ATCCTTATCG ATTTCACCAA GCGTGGGCTG CTCTTCCGTG GCCGCATCTC AGAGCGGCTC 
AAGACCAGAG GAATCTTTGA AACCAAGTTC CTGTCTCAGA TTGAGAGTGA CTGCCTGGCC 
CTGCTACAAG TTCGTGCCAT CCTGCGCCAC TTAGGGCTCG AGAGCACCTG TGACGACAGC 
ATCATTGTCA AGGAGGTGTG CACGGTGGTG GCGCGGCGGG CAGCCCAGCT CTGTGGTGCA 
GGCCTGGCTG CTGTGGTGGA CAAAATACGA GAGAACCGTG GACTGGACAC TCTCAAAGTG 
ACGGTGGGCG TGGATGGGAC CCTCTATAAG CTACATCCTC ACTTTGCCAA AGTCATGCAT 
GAGACAGTGA AGGACCTGGC TCCGAAATGT GACGTGTCTT TCCTGGCTTC AGAGGACGGC 
AGTGGGAAGG GGGCGGCTCT CATCACTGCT GTGGCCTGTC GCATCCGTGA GGCTGGACAG 
CGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024905232.1
CDS	472..3237
Translation	MIASHLLAYFFTELNHDQVQKVDQYLYHMRLSDETLLEISKRFRKEMEKGLGATTHPTASVKMLPTFVRSTPDGTEHGEFLALDLGGTNFRVLRVRVTDNGLQKVEMENQIYAIPEDLMRGSGTQLFDHIAECLANFMDTLQIKDKKLPLGFTFSFPCIQTKLDESFLVSWTKGFKSSGVEGKDVVTLIRKAIQRRGDFDIDIVAVVNDTVGTMMTCGYDDQNCEIGLIVGTGSNACYMEEMRHIDTVEGDEGRMCINMEWGAFGDDGVLDDIRTEFDHEIDMGSLNPGKQLFEKMISGMYMGELVRLILVKMAKEELLFRGKLSPELLATGRFETKDVSDIEGEKDGIRKAREILVRLGLDPTQEDCVATHRICQIVSTRSANLCAATLAAVLRRIKENKGEERLRSTIGVDGSVYKKHPHFAKRLHKTVRRLVPDCDVRFLRSEDGSGKGAAMVTAVAHRLADQHRARQKTLEPLKLSREQLLEVKRRMKVEMERGLSKETHAIAPVKMLPTYVCATPDGTEKGDFLALDLGGTNFRVLLVRVRNGKRRGVEMHNKIYTIPQEVMHGTGDELFDHIVQCIADFLEYMGMKGVSLPLGFTFSFPCKQNSLDESILLKWTKGFKASGCEGEDVVTLLKEAIHRREEFDLDVVAVVNDTVGTMMTCGYEDPHCEVGLIIGTGCNACYMEEMRNVELVEGDEGRMCVNMEWGAFGDNGCLEDLRTEFDVAVDELSLNFGKQRWSTVS*QMISGMYLGEIVRNILIDFTKRGLLFRGRISERLKTRGIFETKFLSQIESDCLALLQVRAILRHLGLESTCDDSIIVKEVCTVVARRAAQLCGAGLAAVVDKIRENRGLDTLKVTVGVDGTLYKLHPHFAKVMHETVKDLAPKCDVSFLASEDGSGKGAALITAVACRIREAGQR

Target ORF information:

RefSeq Version	XM_025049464.1
Organism	Pteropus alecto(black flying fox)
Definition	Pteropus alecto hexokinase 2 (HK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025049464.1

ORF Insert Sequence:

ATGATCGCCT CGCATCTGCT CGCCTACTTC TTCACGGAGC TCAACCATGA CCAAGTGCAG 
AAGGTTGACC AGTATCTCTA CCATATGCGT CTTTCTGATG AGACCCTTCT GGAGATCTCT 
AAGCGGTTCC GCAAGGAGAT GGAAAAAGGG CTTGGAGCCA CCACCCACCC CACTGCTTCC 
GTGAAAATGC TGCCCACCTT CGTGAGGTCT ACTCCAGACG GGACAGAACA CGGAGAGTTC 
CTGGCTCTAG ACCTTGGAGG GACTAATTTC CGCGTCCTTC GGGTGAGAGT AACGGACAAT 
GGGCTCCAGA AGGTCGAGAT GGAGAACCAG ATCTACGCCA TCCCCGAGGA CCTCATGCGA 
GGCAGCGGCA CCCAGCTGTT CGACCACATT GCTGAATGCC TGGCTAACTT CATGGATACG 
TTACAAATCA AAGATAAGAA GCTCCCCTTG GGCTTTACCT TTTCGTTCCC CTGCATCCAG 
ACAAAACTAG ATGAGAGCTT CCTGGTCTCG TGGACTAAGG GGTTCAAGTC CAGCGGTGTG 
GAAGGCAAAG ATGTGGTTAC TCTAATACGG AAGGCCATCC AGAGGAGAGG GGACTTTGAT 
ATTGATATCG TGGCTGTGGT GAATGACACG GTTGGGACCA TGATGACCTG TGGTTATGAT 
GACCAGAACT GCGAGATTGG TCTCATTGTG GGCACGGGCA GCAACGCCTG CTACATGGAG 
GAGATGCGCC ACATTGACAC AGTGGAAGGT GACGAGGGCC GGATGTGCAT CAACATGGAG 
TGGGGCGCCT TCGGGGACGA CGGCGTGCTT GATGACATCC GTACCGAGTT TGACCACGAG 
ATCGACATGG GCTCCTTGAA CCCCGGGAAG CAACTATTTG AGAAGATGAT CAGTGGGATG 
TACATGGGGG AACTGGTGAG GCTAATCCTG GTGAAGATGG CCAAGGAGGA GCTGCTTTTC 
AGAGGGAAGC TCAGCCCCGA ACTCCTTGCC ACAGGCCGCT TTGAGACAAA AGATGTTTCC 
GACATAGAAG GGGAGAAGGA TGGCATCCGG AAGGCTCGCG AGATCCTGGT GCGGCTGGGC 
TTGGACCCGA CACAGGAGGA CTGCGTGGCC ACCCACCGGA TCTGCCAGAT TGTGTCCACG 
CGCTCAGCCA ACCTGTGCGC GGCCACCCTG GCAGCCGTGC TGCGGCGCAT CAAGGAGAAC 
AAGGGCGAGG AGCGGCTGCG GTCCACCATC GGGGTGGACG GCTCCGTCTA CAAGAAGCAC 
CCCCATTTCG CCAAGCGTCT TCACAAGACT GTGCGGCGCC TGGTGCCCGA CTGCGATGTC 
CGCTTCCTCC GCTCTGAGGA TGGCAGTGGC AAGGGGGCAG CCATGGTGAC GGCAGTGGCC 
CACCGGCTGG CCGACCAACA CCGAGCCCGC CAGAAGACCC TGGAGCCTCT GAAGCTGAGC 
CGTGAGCAGT TGCTGGAGGT CAAGAGGAGG ATGAAGGTAG AAATGGAGCG AGGTCTGAGC 
AAGGAGACTC ACGCCATTGC CCCCGTCAAG ATGCTGCCCA CCTACGTGTG TGCCACCCCT 
GATGGCACAG AGAAAGGTGA CTTCCTGGCC TTGGACCTTG GGGGCACCAA TTTCCGGGTC 
CTGCTGGTGC GTGTGCGGAA TGGGAAGCGG CGCGGAGTGG AGATGCATAA CAAGATCTAC 
ACCATCCCGC AGGAGGTCAT GCACGGCACA GGGGACGAGC TCTTCGACCA CATTGTCCAG 
TGCATCGCCG ACTTCCTTGA GTACATGGGC ATGAAGGGCG TGTCTCTGCC GCTGGGCTTC 
ACCTTCTCCT TCCCGTGCAA ACAGAACAGC CTGGACGAGA GCATCCTCCT CAAGTGGACT 
AAAGGCTTCA AGGCATCTGG CTGTGAGGGC GAGGATGTGG TCACCCTGCT GAAGGAAGCC 
ATCCACCGGC GAGAGGAGTT CGACCTGGAT GTGGTAGCCG TGGTGAATGA CACAGTCGGG 
ACTATGATGA CCTGTGGCTA TGAAGACCCT CACTGTGAAG TCGGCCTCAT CATCGGCACG 
GGCTGCAACG CCTGCTACAT GGAGGAGATG AGGAATGTGG AGCTGGTGGA AGGGGACGAG 
GGGCGGATGT GTGTCAACAT GGAGTGGGGG GCCTTTGGGG ACAATGGATG CCTAGAGGAC 
CTCCGCACGG AATTTGATGT GGCTGTGGAT GAGCTGTCCC TCAACTTTGG CAAACAGAGG 
TGGTCCACTG TATCTTAGCA AATGATCAGT GGCATGTACT TGGGCGAGAT TGTCCGTAAT 
ATCCTTATCG ATTTCACCAA GCGTGGGCTG CTCTTCCGTG GCCGCATCTC AGAGCGGCTC 
AAGACCAGAG GAATCTTTGA AACCAAGTTC CTGTCTCAGA TTGAGAGTGA CTGCCTGGCC 
CTGCTACAAG TTCGTGCCAT CCTGCGCCAC TTAGGGCTCG AGAGCACCTG TGACGACAGC 
ATCATTGTCA AGGAGGTGTG CACGGTGGTG GCGCGGCGGG CAGCCCAGCT CTGTGGTGCA 
GGCCTGGCTG CTGTGGTGGA CAAAATACGA GAGAACCGTG GACTGGACAC TCTCAAAGTG 
ACGGTGGGCG TGGATGGGAC CCTCTATAAG CTACATCCTC ACTTTGCCAA AGTCATGCAT 
GAGACAGTGA AGGACCTGGC TCCGAAATGT GACGTGTCTT TCCTGGCTTC AGAGGACGGC 
AGTGGGAAGG GGGCGGCTCT CATCACTGCT GTGGCCTGTC GCATCCGTGA GGCTGGACAG 
CGATAG